Lag0031568 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0031568
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	TENA_THI-4 domain-containing protein
Location	chr11: 10131550 .. 10132441 (-)
RNA-Seq Expression	Lag0031568
Synteny	Lag0031568

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGGACCCGAAAACCAGAGCTCAACTCTGCGGAGGATTGACCACCACCGACTCATGGATCAGAAAGCACCGTCTCATCTACACCGGAGCCACTCACCATTCGCGACGGCACCGTCGATCTCTCTGCCTTTAGAACTTGGGTGGTAAGTTTACAACAACTAACTCAAAAATTAGGTCTTCGAAAACGACCAATTTCTCAAAGTAATTAGAAGAATTCGAGTTCAAATTCTTCAGGAACAAGAATGCGAATTCCTGAGATCCTTCGCGGCGTTCGTCGGAAGCGTGTTGGTGAAGGCATGGAAAGAATCGGACGACAGAGCGGACGAGGAGGTGATTCTTGGAAGTTTGGCTTCTCTTAACGAAGAATTTGGGTGGTTCAAGAAAGAAGCCCTAAAACGAGACATCAATTTGACTGAAATCCTTCCTCAGAAGGCAACCGCCGACTATTCCAGGTAAGGGTGTTCGTCAGTCGATTTTAAGAGCTCTTATGAACAACCCTAATTTCAAGTATGTCACTAAAATTGCTTGAATTTTTATTGAGAGAATTTGTTGTGAATGAATGATTTAGGTTTCTGGAGAGTGTGATGCGGCCAGAAGTGGAATACACGGTGGCGATGACGGCTCTTTGGGCGATCGAAGCTGTGTACCATGAGAGCTTTGCGTACTGCTTGGAAGATGGATCGAAAACACCATTGGAACTGAGAGAAGCGTGTGAAAGATGGGGGAATGAAGGATTTGGAAAGTACTGCAACACTTTGAAGAAGATTGTGGATAGGCGATTGGAGATGGCGACGGGAGAAGTGGGTAAAAAAGCAGAGGTGGCTTTGTTGAGTGTTCTTGAATACGAAGTTGGGTTTTGGAATATGAATCGTGCTGATCGAAAAACATAA

mRNA sequence

ATGGCGGACCCGAAAACCAGAGCTCAACTCTGCGGAGGATTGACCACCACCGACTCATGGATCAGAAAGCACCGTCTCATCTACACCGGAGCCACTCACCATTCGCGACGGCACCTAATTAGAAGAATTCGAGTTCAAATTCTTCAGGAACAAGAATGCGAATTCCTGAGATCCTTCGCGGCGTTCGTCGGAAGCGTGTTGGTGAAGGCATGGAAAGAATCGGACGACAGAGCGGACGAGGAGGTGATTCTTGGAAGTTTGGCTTCTCTTAACGAAGAATTTGGGTGGTTCAAGAAAGAAGCCCTAAAACGAGACATCAATTTGACTGAAATCCTTCCTCAGAAGGCAACCGCCGACTATTCCAGGTTTCTGGAGAGTGTGATGCGGCCAGAAGTGGAATACACGGTGGCGATGACGGCTCTTTGGGCGATCGAAGCTGTGTACCATGAGAGCTTTGCGTACTGCTTGGAAGATGGATCGAAAACACCATTGGAACTGAGAGAAGCGTGTGAAAGATGGGGGAATGAAGGATTTGGAAAGTACTGCAACACTTTGAAGAAGATTGTGGATAGGCGATTGGAGATGGCGACGGGAGAAGTGGGTAAAAAAGCAGAGGTGGCTTTGTTGAGTGTTCTTGAATACGAAGTTGGGTTTTGGAATATGAATCGTGCTGATCGAAAAACATAA

Coding sequence (CDS)

ATGGCGGACCCGAAAACCAGAGCTCAACTCTGCGGAGGATTGACCACCACCGACTCATGGATCAGAAAGCACCGTCTCATCTACACCGGAGCCACTCACCATTCGCGACGGCACCTAATTAGAAGAATTCGAGTTCAAATTCTTCAGGAACAAGAATGCGAATTCCTGAGATCCTTCGCGGCGTTCGTCGGAAGCGTGTTGGTGAAGGCATGGAAAGAATCGGACGACAGAGCGGACGAGGAGGTGATTCTTGGAAGTTTGGCTTCTCTTAACGAAGAATTTGGGTGGTTCAAGAAAGAAGCCCTAAAACGAGACATCAATTTGACTGAAATCCTTCCTCAGAAGGCAACCGCCGACTATTCCAGGTTTCTGGAGAGTGTGATGCGGCCAGAAGTGGAATACACGGTGGCGATGACGGCTCTTTGGGCGATCGAAGCTGTGTACCATGAGAGCTTTGCGTACTGCTTGGAAGATGGATCGAAAACACCATTGGAACTGAGAGAAGCGTGTGAAAGATGGGGGAATGAAGGATTTGGAAAGTACTGCAACACTTTGAAGAAGATTGTGGATAGGCGATTGGAGATGGCGACGGGAGAAGTGGGTAAAAAAGCAGAGGTGGCTTTGTTGAGTGTTCTTGAATACGAAGTTGGGTTTTGGAATATGAATCGTGCTGATCGAAAAACATAA

Protein sequence

MADPKTRAQLCGGLTTTDSWIRKHRLIYTGATHHSRRHLIRRIRVQILQEQECEFLRSFAAFVGSVLVKAWKESDDRADEEVILGSLASLNEEFGWFKKEALKRDINLTEILPQKATADYSRFLESVMRPEVEYTVAMTALWAIEAVYHESFAYCLEDGSKTPLELREACERWGNEGFGKYCNTLKKIVDRRLEMATGEVGKKAEVALLSVLEYEVGFWNMNRADRKT

Homology

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BLAST of Lag0031568 vs. NCBI nr
Match: XP_023514083.1 (bifunctional TENA-E protein isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 368.2 bits (944), Expect = 5.0e-98
Identity = 184/228 (80.70%), Postives = 196/228 (85.96%), Query Frame = 0


Query: 1   MADPKTRAQLCGGLTTTDSWIRKHRLIYTGATHHSRRHLIRRIRVQI-----LQEQECEF 60
           MADPKTRAQL GG+T TDSWIRKHRLIYT AT H     IR   V +       EQECEF
Sbjct: 1   MADPKTRAQLAGGMTATDSWIRKHRLIYTDATRHPLVLSIRDGTVDLNAFRTWVEQECEF 60

Query: 61  LRSFAAFVGSVLVKAWKESDDRADEEVILGSLASLNEEFGWFKKEALKRDINLTEILPQK 120
           LRSF AFV SVLVKAWKESDDRADEEVILGSLASLN+EF WFKKEALKRDI+LT+I+PQ 
Sbjct: 61  LRSFTAFVASVLVKAWKESDDRADEEVILGSLASLNDEFAWFKKEALKRDIDLTKIVPQN 120

Query: 121 ATADYSRFLESVMRPEVEYTVAMTALWAIEAVYHESFAYCLEDGSKTPLELREACERWGN 180
           ATA YSRFLES+MRPE+EYTVA+TALWAIEAVYHESFAYC+EDGSKTPLELREACERWGN
Sbjct: 121 ATAGYSRFLESLMRPEMEYTVAITALWAIEAVYHESFAYCMEDGSKTPLELREACERWGN 180

Query: 181 EGFGKYCNTLKKIVDRRLEMATGEVGKKAEVALLSVLEYEVGFWNMNR 224
           EGFG YCNTLKKIVDRRLEMA GE+ KK EVALL VLE EV FWNM+R
Sbjct: 181 EGFGNYCNTLKKIVDRRLEMAAGEISKKTEVALLRVLECEVAFWNMSR 228

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BLAST of Lag0031568 vs. NCBI nr
Match: XP_008458358.1 (PREDICTED: probable bifunctional TENA-E protein isoform X1 [Cucumis melo] >TYK02948.1 putative bifunctional TENA-E protein isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 366.3 bits (939), Expect = 1.9e-97
Identity = 183/228 (80.26%), Postives = 197/228 (86.40%), Query Frame = 0


Query: 1   MADPKTRAQLCGGLTTTDSWIRKHRLIYTGATHHSRRHLIRRIRVQILQ-----EQECEF 60
           MAD K RAQL G +T TDSW+RKHRLIYTGAT H     IR   V +       EQECEF
Sbjct: 1   MADTKARAQLAGAMTATDSWLRKHRLIYTGATRHPFILTIRDGTVDLSAFKTWLEQECEF 60

Query: 61  LRSFAAFVGSVLVKAWKESDDRADEEVILGSLASLNEEFGWFKKEALKRDINLTEILPQK 120
           LRSFAAFVGSVLVKAWKESDDRADEEVILGSLA+LN+EF WFKKEALKRDINL+EI+PQK
Sbjct: 61  LRSFAAFVGSVLVKAWKESDDRADEEVILGSLAALNDEFAWFKKEALKRDINLSEIVPQK 120

Query: 121 ATADYSRFLESVMRPEVEYTVAMTALWAIEAVYHESFAYCLEDGSKTPLELREACERWGN 180
           ATA YSRFLES+MRPEVEYTVA+TALWAIEAVYHESFAYCLE+G+KTPLELREACERWG+
Sbjct: 121 ATAGYSRFLESLMRPEVEYTVAITALWAIEAVYHESFAYCLEEGTKTPLELREACERWGS 180

Query: 181 EGFGKYCNTLKKIVDRRLEMATGEVGKKAEVALLSVLEYEVGFWNMNR 224
           EGF KYC+TLKKI DRRLEM +GEV KKAEV LL VLEYEVGFWNM R
Sbjct: 181 EGFDKYCSTLKKIADRRLEMGSGEVNKKAEVGLLRVLEYEVGFWNMIR 228

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BLAST of Lag0031568 vs. NCBI nr
Match: XP_038875310.1 (probable bifunctional TENA-E protein [Benincasa hispida])

HSP 1 Score: 366.3 bits (939), Expect = 1.9e-97
Identity = 184/228 (80.70%), Postives = 195/228 (85.53%), Query Frame = 0


Query: 1   MADPKTRAQLCGGLTTTDSWIRKHRLIYTGATHHSRRHLIRRIRV-----QILQEQECEF 60
           MADPKTRAQL GG+T TDSW+RKHRLIYT AT H     IR   V     +I  EQECEF
Sbjct: 1   MADPKTRAQLAGGMTATDSWLRKHRLIYTEATRHPFVLTIRDGTVDLSAFRIWLEQECEF 60

Query: 61  LRSFAAFVGSVLVKAWKESDDRADEEVILGSLASLNEEFGWFKKEALKRDINLTEILPQK 120
           LRSFAAFV SVLVKAWKESDDRADEEVILGSLASLN+EF WFKKEALKRDINLTEI+PQK
Sbjct: 61  LRSFAAFVASVLVKAWKESDDRADEEVILGSLASLNDEFAWFKKEALKRDINLTEIVPQK 120

Query: 121 ATADYSRFLESVMRPEVEYTVAMTALWAIEAVYHESFAYCLEDGSKTPLELREACERWGN 180
           AT  YSRFLES+MRPEVEYTVA+TALW IEAVYHESFAYC E+G+KTPLELREAC RWGN
Sbjct: 121 ATVGYSRFLESLMRPEVEYTVAITALWGIEAVYHESFAYCSEEGTKTPLELREACGRWGN 180

Query: 181 EGFGKYCNTLKKIVDRRLEMATGEVGKKAEVALLSVLEYEVGFWNMNR 224
           EGFGKYCN LK+I DRRLEM +GEV KKAEV LL VLEYEVGFWNM R
Sbjct: 181 EGFGKYCNRLKEIADRRLEMGSGEVSKKAEVGLLRVLEYEVGFWNMIR 228

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BLAST of Lag0031568 vs. NCBI nr
Match: KAG6594010.1 (putative bifunctional TENA-E protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 365.2 bits (936), Expect = 4.2e-97
Identity = 182/228 (79.82%), Postives = 196/228 (85.96%), Query Frame = 0


Query: 1   MADPKTRAQLCGGLTTTDSWIRKHRLIYTGATHHSRRHLIRRIRVQILQ-----EQECEF 60
           MADPKTRAQL GG+T TDSWIRKHRLIYT AT H     IR   V +       EQ+CEF
Sbjct: 1   MADPKTRAQLAGGMTATDSWIRKHRLIYTDATRHPFVLSIRDGTVDLSAFRTWVEQQCEF 60

Query: 61  LRSFAAFVGSVLVKAWKESDDRADEEVILGSLASLNEEFGWFKKEALKRDINLTEILPQK 120
           LRSF AFV SVLVKAWKESDDRADEEVILGSLA+LN+EF WFKKEALKRDI+LT+I+PQ 
Sbjct: 61  LRSFTAFVASVLVKAWKESDDRADEEVILGSLATLNDEFAWFKKEALKRDIDLTKIVPQN 120

Query: 121 ATADYSRFLESVMRPEVEYTVAMTALWAIEAVYHESFAYCLEDGSKTPLELREACERWGN 180
           ATA YSRFLES+MRPE+EYTVA+TALWAIEAVYHESFAYC+EDGSKTPLELREACERWGN
Sbjct: 121 ATAGYSRFLESLMRPEMEYTVAITALWAIEAVYHESFAYCMEDGSKTPLELREACERWGN 180

Query: 181 EGFGKYCNTLKKIVDRRLEMATGEVGKKAEVALLSVLEYEVGFWNMNR 224
           EGFG YCNTLKKIVDRRLEMA GE+ KK EVALL VLE EV FWNM+R
Sbjct: 181 EGFGNYCNTLKKIVDRRLEMAAGEISKKTEVALLRVLECEVEFWNMSR 228

[Close]

BLAST of Lag0031568 vs. NCBI nr
Match: XP_022138361.1 (probable bifunctional TENA-E protein [Momordica charantia])

HSP 1 Score: 363.2 bits (931), Expect = 1.6e-96
Identity = 182/230 (79.13%), Postives = 194/230 (84.35%), Query Frame = 0


Query: 1   MADPKTRAQLCGGLTTTDSWIRKHRLIYTGATHHSRRHLIRRIRVQILQ-----EQECEF 60
           MADPKTR QL G +  TD+W+RKHRLIY  AT H     IR   +         EQECEF
Sbjct: 1   MADPKTRPQLGGVMAATDAWLRKHRLIYIEATRHPFVLTIRDGTIDFSAFSSWVEQECEF 60

Query: 61  LRSFAAFVGSVLVKAWKESDDRADEEVILGSLASLNEEFGWFKKEALKRDINLTEILPQK 120
           LRSFAAFVGSVLVKAWKESDDRADEEVILGSLASLN+E GWFKKEALKR +NL+EI+PQK
Sbjct: 61  LRSFAAFVGSVLVKAWKESDDRADEEVILGSLASLNDEIGWFKKEALKRGMNLSEIVPQK 120

Query: 121 ATADYSRFLESVMRPEVEYTVAMTALWAIEAVYHESFAYCLEDGSKTPLELREACERWGN 180
           ATA YSRFLES+MRPEVEYTVA+TALWA+EAVYHESFAYCL DGSKTP ELREACERWGN
Sbjct: 121 ATAGYSRFLESLMRPEVEYTVAITALWAMEAVYHESFAYCLGDGSKTPPELREACERWGN 180

Query: 181 EGFGKYCNTLKKIVDRRLEMATGEVGKKAEVALLSVLEYEVGFWNMNRAD 226
           EGFGKYCNTLK I DRR+EMA GEV KKAEVALL VLEYEVGFWNMNR D
Sbjct: 181 EGFGKYCNTLKNIADRRMEMAAGEVAKKAEVALLRVLEYEVGFWNMNRRD 230

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BLAST of Lag0031568 vs. ExPASy Swiss-Prot
Match: Q9SWB6 (Probable bifunctional TENA-E protein OS=Glycine max OX=3847 GN=TENA_E PE=2 SV=1)

HSP 1 Score: 240.7 bits (613), Expect = 1.6e-62
Identity = 118/214 (55.14%), Postives = 152/214 (71.03%), Query Frame = 0


Query: 17  TDSWIRKHRLIYTGATHHS-----RRHLIRRIRVQILQEQECEFLRSFAAFVGSVLVKAW 76
           T++W++KHRL+Y GAT H      R   I     +    Q+  F+R+F  FV SVL+KAW
Sbjct: 15  TETWLKKHRLLYNGATRHPLIISIRDGTINTASFKTWLAQDYLFVRAFVPFVASVLIKAW 74

Query: 77  KESDDRADEEVILGSLASLNEEFGWFKKEALKRDINLTEILPQKATADYSRFLESVMRPE 136
           KESD   D EVILG +ASL +E  WFK EA K  I+L++++PQ+A  +Y   LES+M P+
Sbjct: 75  KESDCSGDMEVILGGMASLEDEISWFKTEANKWGISLSDVVPQQANKNYCGLLESLMSPD 134

Query: 137 VEYTVAMTALWAIEAVYHESFAYCLEDGSKTPLELREACERWGNEGFGKYCNTLKKIVDR 196
            EYTVA+TA WAIE VY ESFA+C+E+GSKTP EL+E C RWGNE FGKYC +L+ I +R
Sbjct: 135 AEYTVAITAFWAIETVYQESFAHCIEEGSKTPPELKETCVRWGNEAFGKYCQSLQNIANR 194

Query: 197 RLEMATGEVGKKAEVALLSVLEYEVGFWNMNRAD 226
            L+ A+ E  KKAEV LLSVLE+EV FWNM+R +
Sbjct: 195 CLQKASDEELKKAEVMLLSVLEHEVEFWNMSRGN 228

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BLAST of Lag0031568 vs. ExPASy Swiss-Prot
Match: Q9ASY9 (Bifunctional TENA-E protein OS=Arabidopsis thaliana OX=3702 GN=TENA_E PE=1 SV=3)

HSP 1 Score: 223.4 bits (568), Expect = 2.6e-57
Identity = 111/211 (52.61%), Postives = 147/211 (69.67%), Query Frame = 0


Query: 18  DSWIRKHRLIYTGATHHSRRHLIRRIRVQILQ-----EQECEFLRSFAAFVGSVLVKAWK 77
           D+WI KHR IYT AT H+    IR   V +        Q+  F+R F  FV SVL++A K
Sbjct: 8   DTWIDKHRSIYTAATRHAFVVSIRDGSVDLSSFRTWLGQDYLFVRRFVPFVASVLIRACK 67

Query: 78  ESDDRADEEVILGSLASLNEEFGWFKKEALKRDINLTEILPQKATADYSRFLESVMRPEV 137
           +S + +D EV+LG +ASLN+E  WFK+E  K D++ + ++PQ+A  +Y RFLE +M  EV
Sbjct: 68  DSGESSDMEVVLGGIASLNDEIEWFKREGSKWDVDFSTVVPQRANQEYGRFLEDLMSSEV 127

Query: 138 EYTVAMTALWAIEAVYHESFAYCLEDGSKTPLELREACERWGNEGFGKYCNTLKKIVDRR 197
           +Y V MTA WAIEAVY ESFA+CLEDG+KTP+EL  AC RWGN+GF +YC+++K I +R 
Sbjct: 128 KYPVIMTAFWAIEAVYQESFAHCLEDGNKTPVELTGACHRWGNDGFKQYCSSVKNIAERC 187

Query: 198 LEMATGEVGKKAEVALLSVLEYEVGFWNMNR 224
           LE A+GEV  +AE  L+ VLE EV FW M+R
Sbjct: 188 LENASGEVLGEAEDVLVRVLELEVAFWEMSR 218

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BLAST of Lag0031568 vs. ExPASy Swiss-Prot
Match: B6TPF2 (Bifunctional TENA2 protein OS=Zea mays OX=4577 GN=TENA2 PE=1 SV=1)

HSP 1 Score: 189.5 bits (480), Expect = 4.2e-47
Identity = 94/212 (44.34%), Postives = 132/212 (62.26%), Query Frame = 0


Query: 16  TTDSWIRKHRLIYTGATHHSRRHLIRRIRVQILQ-----EQECEFLRSFAAFVGSVLVKA 75
           TT +W+ KHR +Y  AT H     IR   V +        Q+  F+R F AF+ SVL+K 
Sbjct: 10  TTAAWMEKHRHMYERATRHPFTVSIRDGTVDMSAFKRWLSQDYLFVREFVAFIASVLLKC 69

Query: 76  WKESDDRADEEVILGSLASLNEEFGWFKKEALKRDINLTEILPQKATADYSRFLESVMRP 135
            K+ +D +D E+ILG +AS+++E  WFK EA    ++L  + P KA  +Y RFL S   P
Sbjct: 70  CKQ-EDSSDMEIILGGVASISDEISWFKNEATVWGVDLASVSPLKANLEYHRFLRSFTEP 129

Query: 136 EVEYTVAMTALWAIEAVYHESFAYCLEDGSKTPLELREACERWGNEGFGKYCNTLKKIVD 195
           E+ Y VA+T  W IE VY +SF +C++DG+KTP EL   C+RWG+ GF +YC +L+ IVD
Sbjct: 130 EISYAVAVTTFWTIETVYQDSFGFCIQDGNKTPPELLGTCQRWGSAGFRQYCQSLQSIVD 189

Query: 196 RRLEMATGEVGKKAEVALLSVLEYEVGFWNMN 223
           R L  A  +  + AE A + VLE E+GFW+M+
Sbjct: 190 RCLANAPADAVQSAEEAFVRVLELEIGFWDMS 220

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BLAST of Lag0031568 vs. ExPASy TrEMBL
Match: A0A5D3BVD1 (Putative bifunctional TENA-E protein isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold46G00300 PE=4 SV=1)

HSP 1 Score: 366.3 bits (939), Expect = 9.2e-98
Identity = 183/228 (80.26%), Postives = 197/228 (86.40%), Query Frame = 0


Query: 1   MADPKTRAQLCGGLTTTDSWIRKHRLIYTGATHHSRRHLIRRIRVQILQ-----EQECEF 60
           MAD K RAQL G +T TDSW+RKHRLIYTGAT H     IR   V +       EQECEF
Sbjct: 1   MADTKARAQLAGAMTATDSWLRKHRLIYTGATRHPFILTIRDGTVDLSAFKTWLEQECEF 60

Query: 61  LRSFAAFVGSVLVKAWKESDDRADEEVILGSLASLNEEFGWFKKEALKRDINLTEILPQK 120
           LRSFAAFVGSVLVKAWKESDDRADEEVILGSLA+LN+EF WFKKEALKRDINL+EI+PQK
Sbjct: 61  LRSFAAFVGSVLVKAWKESDDRADEEVILGSLAALNDEFAWFKKEALKRDINLSEIVPQK 120

Query: 121 ATADYSRFLESVMRPEVEYTVAMTALWAIEAVYHESFAYCLEDGSKTPLELREACERWGN 180
           ATA YSRFLES+MRPEVEYTVA+TALWAIEAVYHESFAYCLE+G+KTPLELREACERWG+
Sbjct: 121 ATAGYSRFLESLMRPEVEYTVAITALWAIEAVYHESFAYCLEEGTKTPLELREACERWGS 180

Query: 181 EGFGKYCNTLKKIVDRRLEMATGEVGKKAEVALLSVLEYEVGFWNMNR 224
           EGF KYC+TLKKI DRRLEM +GEV KKAEV LL VLEYEVGFWNM R
Sbjct: 181 EGFDKYCSTLKKIADRRLEMGSGEVNKKAEVGLLRVLEYEVGFWNMIR 228

[Close]

BLAST of Lag0031568 vs. ExPASy TrEMBL
Match: A0A1S3C775 (probable bifunctional TENA-E protein isoform X1 OS=Cucumis melo OX=3656 GN=LOC103497792 PE=4 SV=1)

HSP 1 Score: 366.3 bits (939), Expect = 9.2e-98
Identity = 183/228 (80.26%), Postives = 197/228 (86.40%), Query Frame = 0


Query: 1   MADPKTRAQLCGGLTTTDSWIRKHRLIYTGATHHSRRHLIRRIRVQILQ-----EQECEF 60
           MAD K RAQL G +T TDSW+RKHRLIYTGAT H     IR   V +       EQECEF
Sbjct: 1   MADTKARAQLAGAMTATDSWLRKHRLIYTGATRHPFILTIRDGTVDLSAFKTWLEQECEF 60

Query: 61  LRSFAAFVGSVLVKAWKESDDRADEEVILGSLASLNEEFGWFKKEALKRDINLTEILPQK 120
           LRSFAAFVGSVLVKAWKESDDRADEEVILGSLA+LN+EF WFKKEALKRDINL+EI+PQK
Sbjct: 61  LRSFAAFVGSVLVKAWKESDDRADEEVILGSLAALNDEFAWFKKEALKRDINLSEIVPQK 120

Query: 121 ATADYSRFLESVMRPEVEYTVAMTALWAIEAVYHESFAYCLEDGSKTPLELREACERWGN 180
           ATA YSRFLES+MRPEVEYTVA+TALWAIEAVYHESFAYCLE+G+KTPLELREACERWG+
Sbjct: 121 ATAGYSRFLESLMRPEVEYTVAITALWAIEAVYHESFAYCLEEGTKTPLELREACERWGS 180

Query: 181 EGFGKYCNTLKKIVDRRLEMATGEVGKKAEVALLSVLEYEVGFWNMNR 224
           EGF KYC+TLKKI DRRLEM +GEV KKAEV LL VLEYEVGFWNM R
Sbjct: 181 EGFDKYCSTLKKIADRRLEMGSGEVNKKAEVGLLRVLEYEVGFWNMIR 228

[Close]

BLAST of Lag0031568 vs. ExPASy TrEMBL
Match: A0A6J1C9H5 (probable bifunctional TENA-E protein OS=Momordica charantia OX=3673 GN=LOC111009554 PE=4 SV=1)

HSP 1 Score: 363.2 bits (931), Expect = 7.8e-97
Identity = 182/230 (79.13%), Postives = 194/230 (84.35%), Query Frame = 0


Query: 1   MADPKTRAQLCGGLTTTDSWIRKHRLIYTGATHHSRRHLIRRIRVQILQ-----EQECEF 60
           MADPKTR QL G +  TD+W+RKHRLIY  AT H     IR   +         EQECEF
Sbjct: 1   MADPKTRPQLGGVMAATDAWLRKHRLIYIEATRHPFVLTIRDGTIDFSAFSSWVEQECEF 60

Query: 61  LRSFAAFVGSVLVKAWKESDDRADEEVILGSLASLNEEFGWFKKEALKRDINLTEILPQK 120
           LRSFAAFVGSVLVKAWKESDDRADEEVILGSLASLN+E GWFKKEALKR +NL+EI+PQK
Sbjct: 61  LRSFAAFVGSVLVKAWKESDDRADEEVILGSLASLNDEIGWFKKEALKRGMNLSEIVPQK 120

Query: 121 ATADYSRFLESVMRPEVEYTVAMTALWAIEAVYHESFAYCLEDGSKTPLELREACERWGN 180
           ATA YSRFLES+MRPEVEYTVA+TALWA+EAVYHESFAYCL DGSKTP ELREACERWGN
Sbjct: 121 ATAGYSRFLESLMRPEVEYTVAITALWAMEAVYHESFAYCLGDGSKTPPELREACERWGN 180

Query: 181 EGFGKYCNTLKKIVDRRLEMATGEVGKKAEVALLSVLEYEVGFWNMNRAD 226
           EGFGKYCNTLK I DRR+EMA GEV KKAEVALL VLEYEVGFWNMNR D
Sbjct: 181 EGFGKYCNTLKNIADRRMEMAAGEVAKKAEVALLRVLEYEVGFWNMNRRD 230

[Close]

BLAST of Lag0031568 vs. ExPASy TrEMBL
Match: A0A1S3C7T1 (probable bifunctional TENA-E protein isoform X2 OS=Cucumis melo OX=3656 GN=LOC103497792 PE=4 SV=1)

HSP 1 Score: 362.5 bits (929), Expect = 1.3e-96
Identity = 181/226 (80.09%), Postives = 196/226 (86.73%), Query Frame = 0


Query: 1   MADPKTRAQLCGGLTTTDSWIRKHRLIYTGATHHSRRHLIRRIRVQILQEQ---ECEFLR 60
           MAD K RAQL G +T TDSW+RKHRLIYTGAT H     IR   V +   +   ECEFLR
Sbjct: 1   MADTKARAQLAGAMTATDSWLRKHRLIYTGATRHPFILTIRDGTVDLSAFKTWLECEFLR 60

Query: 61  SFAAFVGSVLVKAWKESDDRADEEVILGSLASLNEEFGWFKKEALKRDINLTEILPQKAT 120
           SFAAFVGSVLVKAWKESDDRADEEVILGSLA+LN+EF WFKKEALKRDINL+EI+PQKAT
Sbjct: 61  SFAAFVGSVLVKAWKESDDRADEEVILGSLAALNDEFAWFKKEALKRDINLSEIVPQKAT 120

Query: 121 ADYSRFLESVMRPEVEYTVAMTALWAIEAVYHESFAYCLEDGSKTPLELREACERWGNEG 180
           A YSRFLES+MRPEVEYTVA+TALWAIEAVYHESFAYCLE+G+KTPLELREACERWG+EG
Sbjct: 121 AGYSRFLESLMRPEVEYTVAITALWAIEAVYHESFAYCLEEGTKTPLELREACERWGSEG 180

Query: 181 FGKYCNTLKKIVDRRLEMATGEVGKKAEVALLSVLEYEVGFWNMNR 224
           F KYC+TLKKI DRRLEM +GEV KKAEV LL VLEYEVGFWNM R
Sbjct: 181 FDKYCSTLKKIADRRLEMGSGEVNKKAEVGLLRVLEYEVGFWNMIR 226

[Close]

BLAST of Lag0031568 vs. ExPASy TrEMBL
Match: A0A6J1EWW3 (bifunctional TENA-E protein OS=Cucurbita moschata OX=3662 GN=LOC111436879 PE=4 SV=1)

HSP 1 Score: 362.1 bits (928), Expect = 1.7e-96
Identity = 180/228 (78.95%), Postives = 194/228 (85.09%), Query Frame = 0


Query: 1   MADPKTRAQLCGGLTTTDSWIRKHRLIYTGATHHSRRHLIRRIRVQILQ-----EQECEF 60
           MADPKTRAQL GG+T TDSWIRKHRLIYT AT H     IR   V +       EQ+CEF
Sbjct: 1   MADPKTRAQLAGGMTATDSWIRKHRLIYTDATRHPFVLSIRDGTVDLSAFRTWVEQQCEF 60

Query: 61  LRSFAAFVGSVLVKAWKESDDRADEEVILGSLASLNEEFGWFKKEALKRDINLTEILPQK 120
           LRSF AFV SVLVKAWKESDDRADEEVILGSLA+LN+EF WFKKEALKRDI+LT+I+PQ 
Sbjct: 61  LRSFTAFVASVLVKAWKESDDRADEEVILGSLATLNDEFAWFKKEALKRDIDLTKIVPQN 120

Query: 121 ATADYSRFLESVMRPEVEYTVAMTALWAIEAVYHESFAYCLEDGSKTPLELREACERWGN 180
           ATA YSRFLES+MRPE+EYTVA+TALWAIEAVYHESFAYC+EDGS TPLELREACERWGN
Sbjct: 121 ATAGYSRFLESLMRPEMEYTVAITALWAIEAVYHESFAYCMEDGSNTPLELREACERWGN 180

Query: 181 EGFGKYCNTLKKIVDRRLEMATGEVGKKAEVALLSVLEYEVGFWNMNR 224
           EGFG YCNTLKKIVDRRLEM TGE+ KK EV LL VLE EV FWNM+R
Sbjct: 181 EGFGNYCNTLKKIVDRRLEMTTGEISKKTEVVLLRVLECEVEFWNMSR 228

[Close]

BLAST of Lag0031568 vs. TAIR 10
Match: AT3G16990.1 (Haem oxygenase-like, multi-helical )

HSP 1 Score: 223.4 bits (568), Expect = 1.9e-58
Identity = 111/211 (52.61%), Postives = 147/211 (69.67%), Query Frame = 0


Query: 18  DSWIRKHRLIYTGATHHSRRHLIRRIRVQILQ-----EQECEFLRSFAAFVGSVLVKAWK 77
           D+WI KHR IYT AT H+    IR   V +        Q+  F+R F  FV SVL++A K
Sbjct: 8   DTWIDKHRSIYTAATRHAFVVSIRDGSVDLSSFRTWLGQDYLFVRRFVPFVASVLIRACK 67

Query: 78  ESDDRADEEVILGSLASLNEEFGWFKKEALKRDINLTEILPQKATADYSRFLESVMRPEV 137
           +S + +D EV+LG +ASLN+E  WFK+E  K D++ + ++PQ+A  +Y RFLE +M  EV
Sbjct: 68  DSGESSDMEVVLGGIASLNDEIEWFKREGSKWDVDFSTVVPQRANQEYGRFLEDLMSSEV 127

Query: 138 EYTVAMTALWAIEAVYHESFAYCLEDGSKTPLELREACERWGNEGFGKYCNTLKKIVDRR 197
           +Y V MTA WAIEAVY ESFA+CLEDG+KTP+EL  AC RWGN+GF +YC+++K I +R 
Sbjct: 128 KYPVIMTAFWAIEAVYQESFAHCLEDGNKTPVELTGACHRWGNDGFKQYCSSVKNIAERC 187

Query: 198 LEMATGEVGKKAEVALLSVLEYEVGFWNMNR 224
           LE A+GEV  +AE  L+ VLE EV FW M+R
Sbjct: 188 LENASGEVLGEAEDVLVRVLELEVAFWEMSR 218

The following BLAST results are available for this feature:

BLAST of Lag0031568 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023514083.1	5.0e-98	80.70	bifunctional TENA-E protein isoform X2 [Cucurbita pepo subsp. pepo]	[more]
XP_008458358.1	1.9e-97	80.26	PREDICTED: probable bifunctional TENA-E protein isoform X1 [Cucumis melo] >TYK02...	[more]
XP_038875310.1	1.9e-97	80.70	probable bifunctional TENA-E protein [Benincasa hispida]	[more]
KAG6594010.1	4.2e-97	79.82	putative bifunctional TENA-E protein, partial [Cucurbita argyrosperma subsp. sor...	[more]
XP_022138361.1	1.6e-96	79.13	probable bifunctional TENA-E protein [Momordica charantia]	[more]

BLAST of Lag0031568 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SWB6	1.6e-62	55.14	Probable bifunctional TENA-E protein OS=Glycine max OX=3847 GN=TENA_E PE=2 SV=1	[more]
Q9ASY9	2.6e-57	52.61	Bifunctional TENA-E protein OS=Arabidopsis thaliana OX=3702 GN=TENA_E PE=1 SV=3	[more]
B6TPF2	4.2e-47	44.34	Bifunctional TENA2 protein OS=Zea mays OX=4577 GN=TENA2 PE=1 SV=1	[more]

BLAST of Lag0031568 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3BVD1	9.2e-98	80.26	Putative bifunctional TENA-E protein isoform X1 OS=Cucumis melo var. makuwa OX=1...	[more]
A0A1S3C775	9.2e-98	80.26	probable bifunctional TENA-E protein isoform X1 OS=Cucumis melo OX=3656 GN=LOC10...	[more]
A0A6J1C9H5	7.8e-97	79.13	probable bifunctional TENA-E protein OS=Momordica charantia OX=3673 GN=LOC111009...	[more]
A0A1S3C7T1	1.3e-96	80.09	probable bifunctional TENA-E protein isoform X2 OS=Cucumis melo OX=3656 GN=LOC10...	[more]
A0A6J1EWW3	1.7e-96	78.95	bifunctional TENA-E protein OS=Cucurbita moschata OX=3662 GN=LOC111436879 PE=4 S...	[more]

BLAST of Lag0031568 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G16990.1	1.9e-58	52.61	Haem oxygenase-like, multi-helical	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR004305	Thiaminase-2/PQQC	PFAM	PF03070	TENA_THI-4	coord: 51..222 e-value: 1.1E-21 score: 77.7
IPR016084	Haem oxygenase-like, multi-helical	GENE3D	1.20.910.10		coord: 14..223 e-value: 2.4E-39 score: 137.5
IPR016084	Haem oxygenase-like, multi-helical	SUPERFAMILY	48613	Heme oxygenase-like	coord: 51..221
None	No IPR available	PANTHER	PTHR43198	BIFUNCTIONAL TH2 PROTEIN	coord: 12..223
None	No IPR available	PANTHER	PTHR43198:SF5	BIFUNCTIONAL TENA-E PROTEIN	coord: 12..223
None	No IPR available	CDD	cd19357	TenA_E_At3g16990-like	coord: 17..221 e-value: 2.74455E-51 score: 164.028

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0031568.1	Lag0031568.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006772	thiamine metabolic process
cellular_component	GO:0005829	cytosol