Lag0031552 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0031552
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionAMSH-like ubiquitin thioesterase 3
Locationchr11: 9892162 .. 9897584 (-)
RNA-Seq ExpressionLag0031552
SyntenyLag0031552
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAGATCAACATCGATAGAGAGGCGCGGAAAGTTGCGGTTGATAATCGAATTCCCCTCCGGAATTACTATCGGATCGCCGATAATCTCTTAAAACAGGTGATTTTTTCGGAACCGTTCTTATCGATTATTTTGATTGATTTATCGATGCGGAATTGTTAGTGGTTAATGTTTAATTTGAGGTTTTGTTAATTTCCATTTCTTCCTCTACATGGTTGTTGAAGTGATTTTTTATTGCAGTTTTCTTCATTAGTGGTTTAAGTATTAGAATTGCTCACACGTTGCCATGGATCCTTTTCTACGTGCCTCTCATTGTATTTTTGATGTTATTGAGTTGTTCTCTTAAAATGGCAGGCTAATATTTATCGGGAGGAGAATAATGTTGTGGATTTGTTCATTATACTTCTTAGGTTTTCAAGGTACGGCGGGTCTAGTTAATGCTTGTATTTTCAATTGCCAAGGGATGTTTATTCTTGGTGTAGGAAACTCAATTCTCAACTTGTTCTTATTTTCTCAGCTTGGTTTCTGAAACTATACCACGGCATCGTGATTACCAGGTTTTTTTCCCAAAGGAGAAGATATTTTATAAGAAGGTGAGTAAAAACTCCACATTTCCTCACTTCTTTGCAAAGAATGGTGTATGAATGTTGTGTCATTTTATTTCATGTTGTTGTACCAGAAACTGTTGAGTGTAGTAGATGAACTTGAGTCCTTGAAGCCCGAATTTGATCGCCGAGTAAATGAACTGGAGAAAGTGTATGGACGGCCTCGTCTTCCTCCACCTGATGAGCAGGAAAGAGTTTCATACAGTCTGAGGCCGTCTTCCATTGAACGGCCCTATGTGAATGGAAATTATTCATTGGTTTTGGATCAAAAGCAGGTATCAACTCAAAATGTTTGAATGATATTTTCTGCTCTTGCAACTGTATCAGTCGTAAATGGAAGTTCCACTTATGGTTTAGAGTTTTTATTGATTTCTTGTCTTTTGCAGTTTCTGTTTTTTTATTACTTAAAAATTTTCTCCATGAACATGTGATTTTTGTTTTCTTGTGATCGTGCTTCAGTAATTCACATCCTCCCCTCTCCTCTTGATTCTTAGCATGCCAATTCACAACCACAATCTTCATGGAGGTATAATGATGACCGAGGTCAAGTTTCACAATCAAGTTCCTCAAGGATTGAGAATAAGTTCTCAAATCTGTGAGTATACTTTTTGCCCATATATTTCGTACATACTAGTACGTACTCCTCTAGATAATGTAGGGAGATAAATCATTCCAATAGTTATACTTTAGTGTAAGCCAGAAGAAGGTGATTTTGTAGTTCAAGTAGCTAGGTAGAGGGCAGAAGACAGAAATTGTTATGGCTACAACTTGCATCCTGAACTTAGTTGGCTGAAAAAAAGAAAGTATATTGATGATGTCAGAGTTCTTCTGCAACTGGTCTCAAGATTGAATAGAAAGACAGTGTCTTTAGTACAAGAGCTATGTATTGTCGTATCTTGAATGTCTGTGTCTTCTTATCCTATGCTATGGAGGCTGTAAGAATACAATTCTAGGCATACTCGTCTAGTCACTAGTTATAAACATGCACAAATGGATCTGATAATTAGTGCTGGGAATACGCAGGTCTCTTAATGTACCACTTCCCAAACCTGAAACTTTGTCTAGACATTCTATTTTGGGACCTAGTGGTCTTCAGGGCCAATGGAAGGGACCAAGTACAGATAAGGTGAGTTTTTGCACATTCTTTTTTCTTTATGTTATTTCTTTTGTTTCTGCTTACTGTGAAATCCATTAACAGATTAATCCTTTCGTTCTGTACAGATTCACTATCCAAGAAATCAGGACTTTACTCCTGAAGATTCAAGGTTAGAGGCGTGTATAACTTGTATTAGTATATGATACTAATAGTATCTGAAAATTTACACATTTTGTCTTCTGTGTTTTGAATGTTTAGCTTCCTGATGACGAACACTTAATGAGAATGAGATAAATATTTTGATTTTGTTATTTTACTTATTCTCTGATTATGTTTTCTTGAAATATGTATCTGGAACCTTCAAATTCTTGCAGACTGAATCAAATTGAACAATGTGATCTAGTGGCACATAAAGATAGTGGTCCAGGAGGTGTTATGTCGATGATGGAGTCAGTGCTCTCACTAGATGATGGTAGATGGCCTCATCCTGCGGACATGTCTACGCCTCCATTGATAACAGAAGTAAGGGAGGATCCTTTCCCATTGGTCAAACAACCTTCTCCTCCTCCTGTTCTTGCTAAGGTTCAGCAGGAATATGCGACAATTCCCCCATCTAAAGTTGCGGATCCAAGGCCTGGAGTGGCAAAACAATCGCAGGATGGACCTGACTCGTTCCAACGTCTACACATTGTAGGCCTTTTCTCTCGCTCTCTCTCTTTTTTTTTTTTTTTTTTTTTTTTGCTATATAACTTCAGAAAGCAGTTAGTTATTCACTATTTCTTTTGCTATGTTGTGATTCCTTTTGTCATGTCTATGCTGCAAATGTGTATACCTTATAATATGATATTGGTTACTTTTACTCGTTCCTGCAGCCAGTAAAAATGCTGGATGATTTCTTGAGATTGGCTCGAGAAAATACAAATAAGAATCTGGAGACTTGTGGTGTTTTAGCTGGCTCTCTGGTAAGAGCAAATTAATGATTTAATGTATATTCTTAAAGATTTTTTCTAGTATCGTTCTCATACTTCATCATATATTTGCAGAAAAACAGGGTTTTTTATATATCTACGCTCATAATCCCAAAGCAAGAATCAACCTCAGATTCCGTAAGAATTTAATTACTTTTCACTGCACTTAAATGTTTATTTTCCATTTGACTGGTGATTATACTAGCTTTGAACATGTAAGGTGGAAGTTCGCCGTGCATATGAGTTCTAATTGTTTTATTTTCTGCGTATTTTCTTTTACTATGACTTACGTTGGTATGCTATGGAATTGGCTTGAGGGTTACTTGATTGAGCTCTAGACAGTTACCACTGTTGCCTTTAATCAGTACTTAGAAATCATTTTTCGTAATGTTCAGTGCCAAACGTTGAATGAAGAGGAAATATTTGAAGTTCAAGACAGATTATCACTTTTCCCTCTTGGTTGGATCCATGTAAGCTTTCTGGCTCTCTTTGCAATAGATAGTTTATGATTATAGAATTTGGGAACACAAGATTAGAGATATTGTGATGATTATCTGACTTCTATTGGTGCTTCATAAATAATCTGCTTTTGTATTTTCTGTTTATTGCACTTTCTTCATTGTCCCACTATGGGACGCTGATAGAATAAAAGATGAATTGCAGTATTATTATTGCACATTAGAGAAGATTACAATCTAGATGGACATTCGAGAGGTCTGACATTCTAACCTCACACCAAAGATTCTTTTTGTCCAAGAACAGCCACATACAGGCAAACATAACTACCGAGGAGTAAACATTATACAATAGAACTACATATAACCCGTCCATAACTGCCCATAACCACTCATAACAACCCAAACTACCCAAATGACGAACTGTTCTCCCCTTTCTAGTCTTCTTTACCATCCTTGTGTCTTGGCAACGGTGTGACGGACTTGAGGTCTAACAGATGCAGTGCTTTTCTTCTCCTTTCATATCGTTTTAGTAATCTGATTACATTCAGTCTAAAGATAAAAACAAAACTTTTACTTGATAGGATGGTATGCTAAGTTATTATGTCATTTTGTGTTTTATAAATCACTCTTTCAGTAGTCGTCTCATAGATCATCTGATTGAGCGACCTAGTACCTAGAGGCCCCTTAAGAAATGTTTTCCAACTTCTCGCAAGTTTATGTGCAAAACTTCAAGGAATTTGTAAATAGAGGGACTTCAGTTTGTATTAAAATTTACACTATATATTATTGCTTATTGGACTTTATATGGATGGTCAATGCTTTCTGAATAACCATATTAATAAGCTTTGCATTTTTTCACTTAAGTTGACTATGTAGCGATGTCTTGAGATACATGCTCTTGATAAAACTGGAAAGTAAAATTGTGATATATTACTTTCAACATATATGTTCTTGAGAATAGAGGGACTTTAACATATGTGTCAGAGTAAGTATACGTTAATAAGCATATATCCTCGACCAAGAGGTCAGATCTTCGAATCCTTCCACCCTCAATGTTGTTGAACTCAAAAAAAAAAAAAAAAAAAAAAAAAAATTCTTGAGAATATAGAAATCTGTGGCTTACGTTTCCATATAGCTCGTATTATTTACGCCATACAAGAAACTTCCACTTTGCCAGTTTCTGATGCTTGTTTGGTTCTCAGACACACCCATCACAAACCTGTTTCATGTCATCTGTGGATTTGCACACGCATTACTCGTACCAGGTAAGATAAAAATTTTACTCATTTTTGTTTCTTCACAACGATTTTTTTTTTTTTTTTCCTTCCTTCCTGAACACTCACAGAACGGGTTTAGTTATTTTCATAACATAGAAAGTTGCATCATGAAATAAAAGTGCTAACTTGTAAAGACATTAACCTATACATTCATCCGAAACACTTTCGTGGTTTTGGCTGTTTGCATTATTTATTCTTCCTGTTCTAGTTTATTTTGCCTCTTCTAAGGAGATTTTGGCTTCTTCAGATTATGCTACCGGAAGCGATTGCAATCGTCATGGCTCCTACCGATGCATCAAGGTGTTTTCCCCCCTCCATTCATTCAACTTATTAGTAGTTTCTTCACAATTCCCAATTCTCCAATCTTGTATCACTTGTTAACATGTCCCAATGCTGTTACAGTCCATATGGAATATTTCATCTGTCCGATCCGGGCGGCGTGTCAGTCATCCGAAACTGCCAACAGCGTGGATTTCATCCCCATGAGGAGCCTGACGATGGGAGTCCGCTCTATGAGCACTGCTCTCATGTTTTGATGAATCCCAACGCAAAGTTTGACGTTATGGATCTGAGGTAAGATTTTGATTGCAGACATTACTGAAGGGAACTTGTATAGCACCGTGGAAATAGGGTAAAAACTTCACTCCGACGAAAGCTGTGTAAATTTTGTAATTTGCAAATCCAGTTGAGTTACTGCCATTGTTGCGAGACTTCAGGCGGTGGCCATGATGCCTTTGTTGTGCCGAAGATCTCTCTCTCTCTGATTAAGGCTTCTATTTGTTGAAAGAATAGCCTTAAGTAATATAAATGTAGATGATAACTTCATTTCCTTCAATGCTGGGCAGATTTTTCTTTTTCTTTTTTTTTTTTTTTTTTTTTTTGAGAATAGATTTTTCCTTCTTTACATTGGAGGGTTAACATATTTTCAAGTGTGCCTGTATAGCTTGTACGGTGGATGTCATTTGTGATTATGATATTATTGTAGGACTAATGGATGGATCTCTACTCTCTCGGCCTGA

mRNA sequence

ATGAAGATCAACATCGATAGAGAGGCGCGGAAAGTTGCGGTTGATAATCGAATTCCCCTCCGGAATTACTATCGGATCGCCGATAATCTCTTAAAACAGGCTAATATTTATCGGGAGGAGAATAATGTTGTGGATTTGTTCATTATACTTCTTAGGTTTTCAAGCTTGGTTTCTGAAACTATACCACGGCATCGTGATTACCAGGTTTTTTTCCCAAAGGAGAAGATATTTTATAAGAAGAAACTGTTGAGTGTAGTAGATGAACTTGAGTCCTTGAAGCCCGAATTTGATCGCCGAGTAAATGAACTGGAGAAAGTGTATGGACGGCCTCGTCTTCCTCCACCTGATGAGCAGGAAAGAGTTTCATACAGTCTGAGGCCGTCTTCCATTGAACGGCCCTATGTGAATGGAAATTATTCATTGGTTTTGGATCAAAAGCAGCATGCCAATTCACAACCACAATCTTCATGGAGGTATAATGATGACCGAGGTCAAGTTTCACAATCAAGTTCCTCAAGGATTGAGAATAAGTTCTCAAATCTGTCTCTTAATGTACCACTTCCCAAACCTGAAACTTTGTCTAGACATTCTATTTTGGGACCTAGTGGTCTTCAGGGCCAATGGAAGGGACCAAGTACAGATAAGATTCACTATCCAAGAAATCAGGACTTTACTCCTGAAGATTCAAGACTGAATCAAATTGAACAATGTGATCTAGTGGCACATAAAGATAGTGGTCCAGGAGGTGTTATGTCGATGATGGAGTCAGTGCTCTCACTAGATGATGGTAGATGGCCTCATCCTGCGGACATGTCTACGCCTCCATTGATAACAGAAGTAAGGGAGGATCCTTTCCCATTGGTCAAACAACCTTCTCCTCCTCCTGTTCTTGCTAAGGTTCAGCAGGAATATGCGACAATTCCCCCATCTAAAGTTGCGGATCCAAGGCCTGGAGTGGCAAAACAATCGCAGGATGGACCTGACTCGTTCCAACGTCTACACATTCCAGTAAAAATGCTGGATGATTTCTTGAGATTGGCTCGAGAAAATACAAATAAGAATCTGGAGACTTGTGGTGTTTTAGCTGGCTCTCTGAAAAACAGGGTTTTTTATATATCTACGCTCATAATCCCAAAGCAAGAATCAACCTCAGATTCCTGCCAAACGTTGAATGAAGAGGAAATATTTGAAGTTCAAGACAGATTATCACTTTTCCCTCTTGGTTGGATCCATACACACCCATCACAAACCTGTTTCATGTCATCTGTGGATTTGCACACGCATTACTCGTACCAGATTATGCTACCGGAAGCGATTGCAATCGTCATGGCTCCTACCGATGCATCAAGTCCATATGGAATATTTCATCTGTCCGATCCGGGCGGCGTGTCAGTCATCCGAAACTGCCAACAGCGTGGATTTCATCCCCATGAGGAGCCTGACGATGGGAGTCCGCTCTATGAGCACTGCTCTCATGTTTTGATGAATCCCAACGCAAAGTTTGACGTTATGGATCTGAGGACTAATGGATGGATCTCTACTCTCTCGGCCTGA

Coding sequence (CDS)

ATGAAGATCAACATCGATAGAGAGGCGCGGAAAGTTGCGGTTGATAATCGAATTCCCCTCCGGAATTACTATCGGATCGCCGATAATCTCTTAAAACAGGCTAATATTTATCGGGAGGAGAATAATGTTGTGGATTTGTTCATTATACTTCTTAGGTTTTCAAGCTTGGTTTCTGAAACTATACCACGGCATCGTGATTACCAGGTTTTTTTCCCAAAGGAGAAGATATTTTATAAGAAGAAACTGTTGAGTGTAGTAGATGAACTTGAGTCCTTGAAGCCCGAATTTGATCGCCGAGTAAATGAACTGGAGAAAGTGTATGGACGGCCTCGTCTTCCTCCACCTGATGAGCAGGAAAGAGTTTCATACAGTCTGAGGCCGTCTTCCATTGAACGGCCCTATGTGAATGGAAATTATTCATTGGTTTTGGATCAAAAGCAGCATGCCAATTCACAACCACAATCTTCATGGAGGTATAATGATGACCGAGGTCAAGTTTCACAATCAAGTTCCTCAAGGATTGAGAATAAGTTCTCAAATCTGTCTCTTAATGTACCACTTCCCAAACCTGAAACTTTGTCTAGACATTCTATTTTGGGACCTAGTGGTCTTCAGGGCCAATGGAAGGGACCAAGTACAGATAAGATTCACTATCCAAGAAATCAGGACTTTACTCCTGAAGATTCAAGACTGAATCAAATTGAACAATGTGATCTAGTGGCACATAAAGATAGTGGTCCAGGAGGTGTTATGTCGATGATGGAGTCAGTGCTCTCACTAGATGATGGTAGATGGCCTCATCCTGCGGACATGTCTACGCCTCCATTGATAACAGAAGTAAGGGAGGATCCTTTCCCATTGGTCAAACAACCTTCTCCTCCTCCTGTTCTTGCTAAGGTTCAGCAGGAATATGCGACAATTCCCCCATCTAAAGTTGCGGATCCAAGGCCTGGAGTGGCAAAACAATCGCAGGATGGACCTGACTCGTTCCAACGTCTACACATTCCAGTAAAAATGCTGGATGATTTCTTGAGATTGGCTCGAGAAAATACAAATAAGAATCTGGAGACTTGTGGTGTTTTAGCTGGCTCTCTGAAAAACAGGGTTTTTTATATATCTACGCTCATAATCCCAAAGCAAGAATCAACCTCAGATTCCTGCCAAACGTTGAATGAAGAGGAAATATTTGAAGTTCAAGACAGATTATCACTTTTCCCTCTTGGTTGGATCCATACACACCCATCACAAACCTGTTTCATGTCATCTGTGGATTTGCACACGCATTACTCGTACCAGATTATGCTACCGGAAGCGATTGCAATCGTCATGGCTCCTACCGATGCATCAAGTCCATATGGAATATTTCATCTGTCCGATCCGGGCGGCGTGTCAGTCATCCGAAACTGCCAACAGCGTGGATTTCATCCCCATGAGGAGCCTGACGATGGGAGTCCGCTCTATGAGCACTGCTCTCATGTTTTGATGAATCCCAACGCAAAGTTTGACGTTATGGATCTGAGGACTAATGGATGGATCTCTACTCTCTCGGCCTGA

Protein sequence

MKINIDREARKVAVDNRIPLRNYYRIADNLLKQANIYREENNVVDLFIILLRFSSLVSETIPRHRDYQVFFPKEKIFYKKKLLSVVDELESLKPEFDRRVNELEKVYGRPRLPPPDEQERVSYSLRPSSIERPYVNGNYSLVLDQKQHANSQPQSSWRYNDDRGQVSQSSSSRIENKFSNLSLNVPLPKPETLSRHSILGPSGLQGQWKGPSTDKIHYPRNQDFTPEDSRLNQIEQCDLVAHKDSGPGGVMSMMESVLSLDDGRWPHPADMSTPPLITEVREDPFPLVKQPSPPPVLAKVQQEYATIPPSKVADPRPGVAKQSQDGPDSFQRLHIPVKMLDDFLRLARENTNKNLETCGVLAGSLKNRVFYISTLIIPKQESTSDSCQTLNEEEIFEVQDRLSLFPLGWIHTHPSQTCFMSSVDLHTHYSYQIMLPEAIAIVMAPTDASSPYGIFHLSDPGGVSVIRNCQQRGFHPHEEPDDGSPLYEHCSHVLMNPNAKFDVMDLRTNGWISTLSA
Homology
BLAST of Lag0031552 vs. NCBI nr
Match: XP_038875747.1 (AMSH-like ubiquitin thioesterase 3 [Benincasa hispida])

HSP 1 Score: 979.2 bits (2530), Expect = 1.4e-281
Identity = 483/507 (95.27%), Postives = 494/507 (97.44%), Query Frame = 0

Query: 1   MKINIDREARKVAVDNRIPLRNYYRIADNLLKQANIYREENNVVDLFIILLRFSSLVSET 60
           MKINIDREARKVAVDNRIPLRNY+RIADNLLKQA+IYREENNVVDLFIILLRFSSLVSET
Sbjct: 1   MKINIDREARKVAVDNRIPLRNYFRIADNLLKQADIYREENNVVDLFIILLRFSSLVSET 60

Query: 61  IPRHRDYQVFFPKEKIFYKKKLLSVVDELESLKPEFDRRVNELEKVYGRPRLPPPDEQER 120
           IPRHRDYQ FFPKEKIFYKKKLLSVVDELESLKPEFDRRVNELEKVYGRPRLPPPDEQ  
Sbjct: 61  IPRHRDYQAFFPKEKIFYKKKLLSVVDELESLKPEFDRRVNELEKVYGRPRLPPPDEQGS 120

Query: 121 VSYSLRPSSIERPYVNGNYSLVLDQKQHANSQPQSSWRYNDDRGQVSQSSSSRIENKFSN 180
           VS SLRPSS+E+PY NGNYSLVLDQKQH+NS+PQSSWRY DDRG VSQS+S RIENKFS+
Sbjct: 121 VSNSLRPSSMEQPYANGNYSLVLDQKQHSNSRPQSSWRYGDDRGHVSQSNSLRIENKFSS 180

Query: 181 LSLNVPLPKPETLSRHSILGPSGLQGQWKGPSTDKIHYPRNQDFTPEDSRLNQIEQCDLV 240
           LSLNVPLPKPETLSRHSILGPSGLQGQWKGPSTDKI YPRNQDFTPEDSRLNQIEQCDLV
Sbjct: 181 LSLNVPLPKPETLSRHSILGPSGLQGQWKGPSTDKIQYPRNQDFTPEDSRLNQIEQCDLV 240

Query: 241 AHKDSGPGGVMSMMESVLSLDDGRWPHPADMSTPPLITEVREDPFPLVKQPSPPPVLAKV 300
           AHKDSGPGGVMSMMESVLSLDDGRWPHPADMSTPPLITE REDPFPLVKQPSPPPVLAKV
Sbjct: 241 AHKDSGPGGVMSMMESVLSLDDGRWPHPADMSTPPLITEAREDPFPLVKQPSPPPVLAKV 300

Query: 301 QQEYATIPPSKVADPRPGVAKQSQDGPDSFQRLHIPVKMLDDFLRLARENTNKNLETCGV 360
           QQEYATIPPSKVADPRPGVAKQS D  +SFQRLHIPVKMLDDFLRLARENTNKNLETCGV
Sbjct: 301 QQEYATIPPSKVADPRPGVAKQSLDESESFQRLHIPVKMLDDFLRLARENTNKNLETCGV 360

Query: 361 LAGSLKNRVFYISTLIIPKQESTSDSCQTLNEEEIFEVQDRLSLFPLGWIHTHPSQTCFM 420
           LAGSLKNRVFYISTLIIPKQESTSDSCQTLNEEEIFEVQDRLSLFPLGWIHTHPSQTCFM
Sbjct: 361 LAGSLKNRVFYISTLIIPKQESTSDSCQTLNEEEIFEVQDRLSLFPLGWIHTHPSQTCFM 420

Query: 421 SSVDLHTHYSYQIMLPEAIAIVMAPTDASSPYGIFHLSDPGGVSVIRNCQQRGFHPHEEP 480
           SSVDLHTHYSYQIMLPEAIAIVMAPTDASSPYGIFHLSDPGGVSVIRNCQQRGFHPHEEP
Sbjct: 421 SSVDLHTHYSYQIMLPEAIAIVMAPTDASSPYGIFHLSDPGGVSVIRNCQQRGFHPHEEP 480

Query: 481 DDGSPLYEHCSHVLMNPNAKFDVMDLR 508
           +DGSPLYEHCSHVLMNPNAKFDV+DLR
Sbjct: 481 EDGSPLYEHCSHVLMNPNAKFDVIDLR 507

BLAST of Lag0031552 vs. NCBI nr
Match: XP_023006405.1 (AMSH-like ubiquitin thioesterase 3 isoform X1 [Cucurbita maxima])

HSP 1 Score: 964.1 bits (2491), Expect = 4.7e-277
Identity = 479/507 (94.48%), Postives = 489/507 (96.45%), Query Frame = 0

Query: 1   MKINIDREARKVAVDNRIPLRNYYRIADNLLKQANIYREENNVVDLFIILLRFSSLVSET 60
           MKINIDREARKVAVDNRIPLRNY+RIADNLLKQA+IYREENNVVDLFIILLRFSSLVSET
Sbjct: 1   MKINIDREARKVAVDNRIPLRNYFRIADNLLKQADIYREENNVVDLFIILLRFSSLVSET 60

Query: 61  IPRHRDYQVFFPKEKIFYKKKLLSVVDELESLKPEFDRRVNELEKVYGRPRLPPPDEQER 120
           IPRHRDYQ FFPKEKIFYKKKLLSVVDELESLKPEFDRRVNELEKV GRPRLPP DEQ  
Sbjct: 61  IPRHRDYQAFFPKEKIFYKKKLLSVVDELESLKPEFDRRVNELEKVSGRPRLPPSDEQGG 120

Query: 121 VSYSLRPSSIERPYVNGNYSLVLDQKQHANSQPQSSWRYNDDRGQVSQSSSSRIENKFSN 180
           VS SLRPSS+ +PY NGNYSLVLDQKQHANSQPQSSWRY+D RGQVSQSS SRIEN+ SN
Sbjct: 121 VSNSLRPSSMIQPYANGNYSLVLDQKQHANSQPQSSWRYHDGRGQVSQSSYSRIENRLSN 180

Query: 181 LSLNVPLPKPETLSRHSILGPSGLQGQWKGPSTDKIHYPRNQDFTPEDSRLNQIEQCDLV 240
           LSLNVPLPKPETLSRHSILGPSG QGQWKGPSTDKIHYPRNQDFTP DSRLNQIEQCDLV
Sbjct: 181 LSLNVPLPKPETLSRHSILGPSGFQGQWKGPSTDKIHYPRNQDFTPLDSRLNQIEQCDLV 240

Query: 241 AHKDSGPGGVMSMMESVLSLDDGRWPHPADMSTPPLITEVREDPFPLVKQPSPPPVLAKV 300
           AHKDSGPGGVMSMMESVLSLDDGRWPHPAD+S  PLITEVREDPFPLVKQPSPPPVLAKV
Sbjct: 241 AHKDSGPGGVMSMMESVLSLDDGRWPHPADISITPLITEVREDPFPLVKQPSPPPVLAKV 300

Query: 301 QQEYATIPPSKVADPRPGVAKQSQDGPDSFQRLHIPVKMLDDFLRLARENTNKNLETCGV 360
           QQEYATIPPSKVADPRPGVAKQS D  +SFQRLHIPVKMLDDFLRLARENTNKNLETCGV
Sbjct: 301 QQEYATIPPSKVADPRPGVAKQSLDESESFQRLHIPVKMLDDFLRLARENTNKNLETCGV 360

Query: 361 LAGSLKNRVFYISTLIIPKQESTSDSCQTLNEEEIFEVQDRLSLFPLGWIHTHPSQTCFM 420
           LAGSLKNRVFYISTLIIPKQESTSDSCQTLNEEEIFEVQDRLSLFPLGWIHTHPSQTCFM
Sbjct: 361 LAGSLKNRVFYISTLIIPKQESTSDSCQTLNEEEIFEVQDRLSLFPLGWIHTHPSQTCFM 420

Query: 421 SSVDLHTHYSYQIMLPEAIAIVMAPTDASSPYGIFHLSDPGGVSVIRNCQQRGFHPHEEP 480
           SSVDLHTHYSYQIMLPEAIAIVMAPTDASSPYGIFHLSDPGGVSVIRNCQQRGFHPHEEP
Sbjct: 421 SSVDLHTHYSYQIMLPEAIAIVMAPTDASSPYGIFHLSDPGGVSVIRNCQQRGFHPHEEP 480

Query: 481 DDGSPLYEHCSHVLMNPNAKFDVMDLR 508
           +DGSPLYEHCSHVLMNPNAKFDV+DLR
Sbjct: 481 EDGSPLYEHCSHVLMNPNAKFDVIDLR 507

BLAST of Lag0031552 vs. NCBI nr
Match: TYK02941.1 (AMSH-like ubiquitin thioesterase 3 [Cucumis melo var. makuwa])

HSP 1 Score: 960.7 bits (2482), Expect = 5.2e-276
Identity = 474/510 (92.94%), Postives = 489/510 (95.88%), Query Frame = 0

Query: 1   MKINIDREARKVAVDNRIPLRNYYRIADNLLKQANIYREENNVVDLFIILLRFSSLVSET 60
           MKINIDREARKVAVDNRIPLRNY+RIADNLLKQANIYREENNVVDLFIILLRFSSLVSET
Sbjct: 54  MKINIDREARKVAVDNRIPLRNYFRIADNLLKQANIYREENNVVDLFIILLRFSSLVSET 113

Query: 61  IPRHRDYQVFFPKEKIFYKKKLLSVVDELESLKPEFDRRVNELEKVYGRPRLPPPDEQER 120
           IPRHRDYQ FFPKEKI+YKKKLL+VVDELESLKPEFDRRVNELEKVYGRPRLP PDEQ  
Sbjct: 114 IPRHRDYQAFFPKEKIYYKKKLLNVVDELESLKPEFDRRVNELEKVYGRPRLPLPDEQGS 173

Query: 121 VSYSLRPSSIERPYVNGNYSLVLDQKQHANSQPQSSWRYNDDRGQVSQSSSSRIENKFSN 180
           VS SLRPS  E+PY NGNYSL+LDQKQH NS+PQSSWRY+DDRGQVSQSSS R+ENKFS 
Sbjct: 174 VSNSLRPSPKEQPYANGNYSLMLDQKQHVNSRPQSSWRYSDDRGQVSQSSSLRLENKFST 233

Query: 181 LSLNVPLPKPETLSRHSILGPSGLQGQWKGPSTDKIHYPRNQDFTPEDSRLNQIEQCDLV 240
           +SLNVPLPKPETLSRHSILGPSGLQ QW GPSTDKI YPRNQDFTPEDSRLNQ E CDLV
Sbjct: 234 MSLNVPLPKPETLSRHSILGPSGLQCQWMGPSTDKIQYPRNQDFTPEDSRLNQSEHCDLV 293

Query: 241 AHKDSGPGGVMSMMESVLSLDDGRWPHPADMSTPPLITEVREDPFPLVKQPSPPPVLAKV 300
           AHKDSG GGVMSMMESVLSLDDGRWP+PADMSTPPLITEVREDPFPLVKQPSPPPVLAKV
Sbjct: 294 AHKDSGSGGVMSMMESVLSLDDGRWPNPADMSTPPLITEVREDPFPLVKQPSPPPVLAKV 353

Query: 301 QQEYATIPPSKVADPRPGVAKQSQDGPDSFQRLHIPVKMLDDFLRLARENTNKNLETCGV 360
           QQEYATIPPSKVADPRPGVA+QSQD  +SFQRLHIPVKMLDDFLRLARENTNKNLETCGV
Sbjct: 354 QQEYATIPPSKVADPRPGVARQSQDESESFQRLHIPVKMLDDFLRLARENTNKNLETCGV 413

Query: 361 LAGSLKNRVFYISTLIIPKQESTSDSCQTLNEEEIFEVQDRLSLFPLGWIHTHPSQTCFM 420
           LAGSLKNR+FYISTLIIPKQESTSDSCQTLNEEEIFEVQDRLSLFPLGWIHTHPSQTCFM
Sbjct: 414 LAGSLKNRIFYISTLIIPKQESTSDSCQTLNEEEIFEVQDRLSLFPLGWIHTHPSQTCFM 473

Query: 421 SSVDLHTHYSYQIMLPEAIAIVMAPTDASSPYGIFHLSDPGGVSVIRNCQQRGFHPHEEP 480
           SSVDLHTHYSYQIMLPEAIAIVMAPTD SSPYGIFHLSDPGGVSVIRNCQQRGFHPHEEP
Sbjct: 474 SSVDLHTHYSYQIMLPEAIAIVMAPTDTSSPYGIFHLSDPGGVSVIRNCQQRGFHPHEEP 533

Query: 481 DDGSPLYEHCSHVLMNPNAKFDVMDLRTNG 511
           DDGSPLYEHCSHV+MNPNAKFDV+DLRT G
Sbjct: 534 DDGSPLYEHCSHVMMNPNAKFDVIDLRTIG 563

BLAST of Lag0031552 vs. NCBI nr
Match: XP_008458380.1 (PREDICTED: LOW QUALITY PROTEIN: AMSH-like ubiquitin thioesterase 3 [Cucumis melo])

HSP 1 Score: 957.6 bits (2474), Expect = 4.4e-275
Identity = 472/507 (93.10%), Postives = 487/507 (96.06%), Query Frame = 0

Query: 1   MKINIDREARKVAVDNRIPLRNYYRIADNLLKQANIYREENNVVDLFIILLRFSSLVSET 60
           MKINIDREARKVAVDNRIPLRNY+RIADNLLKQANIYREENNVVDLFIILLRFSSLVSET
Sbjct: 1   MKINIDREARKVAVDNRIPLRNYFRIADNLLKQANIYREENNVVDLFIILLRFSSLVSET 60

Query: 61  IPRHRDYQVFFPKEKIFYKKKLLSVVDELESLKPEFDRRVNELEKVYGRPRLPPPDEQER 120
           IPRHRDYQ FFPKEKI+YKKKLL+VVDELESLKPEFDRRVNELEKVYGRPRLP PDEQ  
Sbjct: 61  IPRHRDYQAFFPKEKIYYKKKLLNVVDELESLKPEFDRRVNELEKVYGRPRLPLPDEQGS 120

Query: 121 VSYSLRPSSIERPYVNGNYSLVLDQKQHANSQPQSSWRYNDDRGQVSQSSSSRIENKFSN 180
           VS SLRPS  E+PY NGNYSL+LDQKQH NS+PQSSWRY+DDRGQVSQSSS R+ENKFS 
Sbjct: 121 VSNSLRPSPKEQPYANGNYSLMLDQKQHVNSRPQSSWRYSDDRGQVSQSSSLRLENKFST 180

Query: 181 LSLNVPLPKPETLSRHSILGPSGLQGQWKGPSTDKIHYPRNQDFTPEDSRLNQIEQCDLV 240
           +SLNVPLPKPETLSRHSILGPSGLQ QW GPSTDKI YPRNQDFTPEDSRLNQ E CDLV
Sbjct: 181 MSLNVPLPKPETLSRHSILGPSGLQCQWMGPSTDKIQYPRNQDFTPEDSRLNQSEHCDLV 240

Query: 241 AHKDSGPGGVMSMMESVLSLDDGRWPHPADMSTPPLITEVREDPFPLVKQPSPPPVLAKV 300
           AHKDSG GGVMSMMESVLSLDDGRWP+PADMSTPPLITEVREDPFPLVKQPSPPPVLAKV
Sbjct: 241 AHKDSGSGGVMSMMESVLSLDDGRWPNPADMSTPPLITEVREDPFPLVKQPSPPPVLAKV 300

Query: 301 QQEYATIPPSKVADPRPGVAKQSQDGPDSFQRLHIPVKMLDDFLRLARENTNKNLETCGV 360
           QQEYATIPPSKVADPRPGVA+QSQD  +SFQRLHIPVKMLDDFLRLARENTNKNLETCGV
Sbjct: 301 QQEYATIPPSKVADPRPGVARQSQDESESFQRLHIPVKMLDDFLRLARENTNKNLETCGV 360

Query: 361 LAGSLKNRVFYISTLIIPKQESTSDSCQTLNEEEIFEVQDRLSLFPLGWIHTHPSQTCFM 420
           LAGSLKNR+FYISTLIIPKQESTSDSCQTLNEEEIFEVQDRLSLFPLGWIHTHPSQTCFM
Sbjct: 361 LAGSLKNRIFYISTLIIPKQESTSDSCQTLNEEEIFEVQDRLSLFPLGWIHTHPSQTCFM 420

Query: 421 SSVDLHTHYSYQIMLPEAIAIVMAPTDASSPYGIFHLSDPGGVSVIRNCQQRGFHPHEEP 480
           SSVDLHTHYSYQIMLPEAIAIVMAPTD SSPYGIFHLSDPGGVSVIRNCQQRGFHPHEEP
Sbjct: 421 SSVDLHTHYSYQIMLPEAIAIVMAPTDTSSPYGIFHLSDPGGVSVIRNCQQRGFHPHEEP 480

Query: 481 DDGSPLYEHCSHVLMNPNAKFDVMDLR 508
           DDGSPLYEHCSHV+MNPNAKFDV+DLR
Sbjct: 481 DDGSPLYEHCSHVMMNPNAKFDVIDLR 507

BLAST of Lag0031552 vs. NCBI nr
Match: XP_023549197.1 (AMSH-like ubiquitin thioesterase 3 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 954.1 bits (2465), Expect = 4.8e-274
Identity = 474/507 (93.49%), Postives = 485/507 (95.66%), Query Frame = 0

Query: 1   MKINIDREARKVAVDNRIPLRNYYRIADNLLKQANIYREENNVVDLFIILLRFSSLVSET 60
           MKINIDREARKVAVDNRIPLRNY+RIADNLLKQA+IYREENNVVDLFIILLRFSSLVSET
Sbjct: 1   MKINIDREARKVAVDNRIPLRNYFRIADNLLKQADIYREENNVVDLFIILLRFSSLVSET 60

Query: 61  IPRHRDYQVFFPKEKIFYKKKLLSVVDELESLKPEFDRRVNELEKVYGRPRLPPPDEQER 120
           IPRHRDYQ FFPKEKIFYKKKLLSVVDELESLKPEFDRRVNELEKV GRPRLP  DEQ  
Sbjct: 61  IPRHRDYQAFFPKEKIFYKKKLLSVVDELESLKPEFDRRVNELEKVSGRPRLPLSDEQGG 120

Query: 121 VSYSLRPSSIERPYVNGNYSLVLDQKQHANSQPQSSWRYNDDRGQVSQSSSSRIENKFSN 180
           VS SLRPSS+ +PY NGNYSL LDQKQHANSQPQSSWRY+D RGQVSQSS SRIEN+ SN
Sbjct: 121 VSNSLRPSSMIQPYANGNYSLALDQKQHANSQPQSSWRYHDGRGQVSQSSYSRIENRLSN 180

Query: 181 LSLNVPLPKPETLSRHSILGPSGLQGQWKGPSTDKIHYPRNQDFTPEDSRLNQIEQCDLV 240
           LSLNVPLPKPETLSRHSILGPSG QGQWKGPSTDKIHYPRNQDFTP DSRLNQIEQCDLV
Sbjct: 181 LSLNVPLPKPETLSRHSILGPSGFQGQWKGPSTDKIHYPRNQDFTPLDSRLNQIEQCDLV 240

Query: 241 AHKDSGPGGVMSMMESVLSLDDGRWPHPADMSTPPLITEVREDPFPLVKQPSPPPVLAKV 300
           AHKDSGPGGV+SMMESVLSLDDGRWPHPAD+S  PLITEVREDPFPLVKQP PPPVLAKV
Sbjct: 241 AHKDSGPGGVVSMMESVLSLDDGRWPHPADISITPLITEVREDPFPLVKQPPPPPVLAKV 300

Query: 301 QQEYATIPPSKVADPRPGVAKQSQDGPDSFQRLHIPVKMLDDFLRLARENTNKNLETCGV 360
           QQEYA IPPSKVADPRPGVAKQS D  +SFQRLHIPVKMLDDFLRLARENTNKNLETCGV
Sbjct: 301 QQEYAAIPPSKVADPRPGVAKQSMDESESFQRLHIPVKMLDDFLRLARENTNKNLETCGV 360

Query: 361 LAGSLKNRVFYISTLIIPKQESTSDSCQTLNEEEIFEVQDRLSLFPLGWIHTHPSQTCFM 420
           LAGSLKNRVFYISTLIIPKQESTSDSCQTLNEEEIFEVQDRLSLFPLGWIHTHPSQTCFM
Sbjct: 361 LAGSLKNRVFYISTLIIPKQESTSDSCQTLNEEEIFEVQDRLSLFPLGWIHTHPSQTCFM 420

Query: 421 SSVDLHTHYSYQIMLPEAIAIVMAPTDASSPYGIFHLSDPGGVSVIRNCQQRGFHPHEEP 480
           SSVDLHTHYSYQIMLPEAIAIVMAPTDASSPYGIFHLSDPGGVSVIRNCQQRGFHPHEEP
Sbjct: 421 SSVDLHTHYSYQIMLPEAIAIVMAPTDASSPYGIFHLSDPGGVSVIRNCQQRGFHPHEEP 480

Query: 481 DDGSPLYEHCSHVLMNPNAKFDVMDLR 508
           +DGSPLYEHCSHVLMNPNAKFDV+DLR
Sbjct: 481 EDGSPLYEHCSHVLMNPNAKFDVIDLR 507

BLAST of Lag0031552 vs. ExPASy Swiss-Prot
Match: Q5PNU3 (AMSH-like ubiquitin thioesterase 3 OS=Arabidopsis thaliana OX=3702 GN=AMSH3 PE=1 SV=2)

HSP 1 Score: 579.3 bits (1492), Expect = 4.2e-164
Identity = 310/515 (60.19%), Postives = 388/515 (75.34%), Query Frame = 0

Query: 1   MKINIDREARKVAVDNRIPLRNYYRIADNLLKQANIYREENNVVDLFIILLRFSSLVSET 60
           MKI++++ AR++ VDNRIPLRNYYRIADNLL+QA+IYREE NVVDL+I+LLR+SSL+SET
Sbjct: 1   MKIDLNKVAREIEVDNRIPLRNYYRIADNLLRQASIYREEKNVVDLYIMLLRYSSLISET 60

Query: 61  IPRHRDYQVFFPKEKIFYKKKLLSVVDELESLKPEFDRRVNELEKVYGRPRLPPPDEQER 120
           IP HRDYQ   P+E++  +K+L +V++ELESLKPEF++ V++L +V    R    D    
Sbjct: 61  IPFHRDYQASLPQERLGSRKRLRAVINELESLKPEFNQLVDKLNRVEDESRQDGSD-LPV 120

Query: 121 VSYSLRPSSIERPYVN-GNYSLVLDQKQHANSQPQSSWRYNDDRGQVSQSSSSRIENKFS 180
           VSYS    ++E P  +  +YS     K    SQP  SW YN++    S S+ ++I+ +F 
Sbjct: 121 VSYS--SDAVEWPPAHKASYSRPDINKPLPTSQP--SWTYNNN--LTSSSNRTQIDQQFQ 180

Query: 181 NLSLNVPLPKPETLSRHSILGPSGLQGQWKGPSTD-KIHYPRNQDF-TPEDSRLNQIEQC 240
            LS +   P   TLSRHS LGP+GL+ Q   P ++ K+ YP N D+ + ++S L +    
Sbjct: 181 KLSFDFLPPNQATLSRHSFLGPNGLKRQMVAPKSEIKVQYPSNTDWGSADNSGLIEAGPS 240

Query: 241 DLVAHKDSGPGGVMSMMESVLSLDDGRWPHPADMSTPPLITEVREDPFPLV--KQPSPPP 300
              A   +G    +S + SVLSLDDGRW   ++      I++  EDPF  V  KQPSPPP
Sbjct: 241 SSSASL-NGDSQEVSTLNSVLSLDDGRWQRHSEAVNSQFISDATEDPFQFVGMKQPSPPP 300

Query: 301 VLAKVQQEYATIPPSKVADPRPGVA---KQSQDGPDSFQRLHIPVKMLDDFLRLARENTN 360
           VLA+V QE A I PSKVADPRPG A    + ++G +S+Q LH+PV+++DDFLRLAR NT 
Sbjct: 301 VLAQVHQELAQICPSKVADPRPGPAIPSLEGKEGSNSYQHLHVPVRIMDDFLRLARSNTE 360

Query: 361 KNLETCGVLAGSLKNRVFYISTLIIPKQESTSDSCQTLNEEEIFEVQDRLSLFPLGWIHT 420
           +NLETCGVLAGSLKNRVF+I+TLIIPKQESTSDSCQTLNEEEIFEVQDRLSLFPLGWIHT
Sbjct: 361 RNLETCGVLAGSLKNRVFHITTLIIPKQESTSDSCQTLNEEEIFEVQDRLSLFPLGWIHT 420

Query: 421 HPSQTCFMSSVDLHTHYSYQIMLPEAIAIVMAPTDASSPYGIFHLSDPGGVSVIRNCQQR 480
           HP+QTCFMSSVDLHTHYSYQIMLPEA+AIVMAPTD S+P+GIFHLSDP GVSVIRNCQQR
Sbjct: 421 HPTQTCFMSSVDLHTHYSYQIMLPEAVAIVMAPTDESTPHGIFHLSDPSGVSVIRNCQQR 480

Query: 481 GFHPHEEPDDGSPLYEHCSHVLMNPNAKFDVMDLR 508
           GFHPHEE +DG+P+YEHCSHV +N   K++V+DLR
Sbjct: 481 GFHPHEESEDGNPIYEHCSHVFLNAKLKYEVLDLR 507

BLAST of Lag0031552 vs. ExPASy Swiss-Prot
Match: Q8VYB5 (AMSH-like ubiquitin thioesterase 1 OS=Arabidopsis thaliana OX=3702 GN=AMSH1 PE=2 SV=1)

HSP 1 Score: 435.6 bits (1119), Expect = 7.6e-121
Identity = 249/514 (48.44%), Postives = 327/514 (63.62%), Query Frame = 0

Query: 3   INIDREARKVAVDNRIPLRNYYRIADNLLKQANIYREENNVVDLFIILLRFSSLVSETIP 62
           I+I   AR++ VDNRI L+ Y+RIADN+LKQANI+R E NV+DL+++LLRFSSL  ETIP
Sbjct: 8   IDIATSARRIGVDNRISLKFYFRIADNILKQANIFRAEKNVIDLYVMLLRFSSLALETIP 67

Query: 63  RHRDYQVFFPKEKIFYKKKLLSVVDELESLKPEFDRRVNELEKVYGRPRLPP--PDEQER 122
            HRDY+      K + + +LL V+ ELE LKP   +R++EL      P+L P    +   
Sbjct: 68  SHRDYRTSLKSNKEYLRMRLLDVLTELEKLKPVVQQRIDEL-----YPKLKPRYNVQAHP 127

Query: 123 VSYSLRPSSIERPYVNGNYSLVLDQKQHANSQPQSSWRYNDDRGQVSQSSSSRIENKFSN 182
            + SL  SS  +P  N              + P  +  Y   RGQ    +++ +E +F  
Sbjct: 128 ANGSLGWSSAVKPSFNSY------DHAKVRNPPGHNSGYMGSRGQ-QFLNAAPLEERFRK 187

Query: 183 LSLNVPLPKPETLSRHSILGPSGLQGQWKGPSTD-KIHYPRNQDFTPE--DSRLNQIEQC 242
           +S+N   P  ETLS+HSILGP GL  QW+ P  D K+ YP N DF+P    S    ++  
Sbjct: 188 MSVNF-RPNEETLSKHSILGPGGLSAQWQPPKYDTKVQYPSNIDFSPVVIPSFQQLVDSK 247

Query: 243 DLVAHKDSGPGGVMSMMESVLSLDDGRWPHPADMSTPPLITEVRE-DPFPLVKQPSPPPV 302
            ++ +  +       +  SV S +  +  +  ++S+     E    +   L++QPSPPPV
Sbjct: 248 PMITNGSNDEPEKPIVEPSVASNEKIQKNYTEELSSMISFEEPESVNENNLIRQPSPPPV 307

Query: 303 LAKVQQEYATIPPSKVADPRPGVAKQSQD---GPDSFQRLHIPVKMLDDFLRLARENTNK 362
           LA+VQ     + P +V +P   +     D     +S   LHI   M+D F+RLA+ NT K
Sbjct: 308 LAEVQDLVPALCP-EVREPECMIENSLPDESLRSESPLELHIATSMMDTFMRLAKSNTKK 367

Query: 363 NLETCGVLAGSLKNRVFYISTLIIPKQESTSDSCQTLNEEEIFEVQDRLSLFPLGWIHTH 422
           NLETCG+LAGSLKNR FYI+ LIIPKQESTSDSCQ  NEEEIFEVQD+ SLFPLGWIHTH
Sbjct: 368 NLETCGILAGSLKNRKFYITALIIPKQESTSDSCQATNEEEIFEVQDKQSLFPLGWIHTH 427

Query: 423 PSQTCFMSSVDLHTHYSYQIMLPEAIAIVMAPTDASSPYGIFHLSDPGGVSVIRNCQQRG 482
           P+Q+CFMSS+D+HTHYSYQIMLPEA+AIVMAP D+S  +GIF L+ PGG++VIRNC +RG
Sbjct: 428 PTQSCFMSSIDVHTHYSYQIMLPEAVAIVMAPQDSSRNHGIFRLTTPGGMTVIRNCDRRG 487

Query: 483 FHPHEEPDDGSPLYEHCSHVLMNPNAKFDVMDLR 508
           FH H  P+DG P+Y  C  V MNPN KFDV+DLR
Sbjct: 488 FHAHSSPEDGGPIYNTCKEVYMNPNLKFDVIDLR 507

BLAST of Lag0031552 vs. ExPASy Swiss-Prot
Match: Q6NKP9 (AMSH-like ubiquitin thioesterase 2 OS=Arabidopsis thaliana OX=3702 GN=AMSH2 PE=2 SV=1)

HSP 1 Score: 265.4 bits (677), Expect = 1.4e-69
Identity = 113/175 (64.57%), Postives = 145/175 (82.86%), Query Frame = 0

Query: 333 LHIPVKMLDDFLRLARENTNKNLETCGVLAGSLKNRVFYISTLIIPKQESTSDSCQTLNE 392
           +HI  ++L+DF  LARENT K+LETCG LA  L+  +FY++TLIIPKQESTS+SCQ +NE
Sbjct: 49  VHISERLLEDFTELARENTEKDLETCGTLAAFLERGIFYVTTLIIPKQESTSNSCQAMNE 108

Query: 393 EEIFEVQDRLSLFPLGWIHTHPSQTCFMSSVDLHTHYSYQIMLPEAIAIVMAPTDASSPY 452
            E+F +Q+   L+P+GWIHTHPSQ CFMSSVDLHTHYSYQ+M+PEA AIV+APTD+S  Y
Sbjct: 109 VEVFSIQNERELYPVGWIHTHPSQGCFMSSVDLHTHYSYQVMVPEAFAIVVAPTDSSKSY 168

Query: 453 GIFHLSDPGGVSVIRNCQQRGFHPHEEPDDGSPLYEHCSHVLMNPNAKFDVMDLR 508
           GIF L+DPGG+ V+R C + GFHPH+EP+DG+P+YEHCS+V  N N +F++ DLR
Sbjct: 169 GIFKLTDPGGMEVLRGCSETGFHPHKEPEDGNPVYEHCSNVYKNSNLRFEIFDLR 223

BLAST of Lag0031552 vs. ExPASy Swiss-Prot
Match: O95630 (STAM-binding protein OS=Homo sapiens OX=9606 GN=STAMBP PE=1 SV=1)

HSP 1 Score: 190.3 bits (482), Expect = 5.6e-47
Identity = 156/503 (31.01%), Postives = 227/503 (45.13%), Query Frame = 0

Query: 12  VAVDNRIPLRNYYRIADNLLKQANIYREENNVVDLFIILLRFSSLVSETIPRHRDYQ-VF 71
           V V+  IP R Y+R    +++ A+IY EE N+   FI+  ++ +L  E +P+HRDY+   
Sbjct: 25  VEVNEDIPPRRYFRSGVEIIRMASIYSEEGNIEHAFILYNKYITLFIEKLPKHRDYKSAV 84

Query: 72  FPKEKIFYKKKLLSVVDELESLKPEFDRRVNELEKVYGRPRLPPPDEQERVSYSLRPSSI 131
            P++K   KK       + E LK E  +R  +    Y        +E+++ +  L  +  
Sbjct: 85  IPEKKDTVKKLKEIAFPKAEELKAELLKRYTKEYTEYN-------EEKKKEAEELARNMA 144

Query: 132 ERPYVNGNYSLVLDQKQHANSQPQSSWRYNDDRGQVSQSSSSRIENKFSNLSLNVPLPKP 191
            +  +      V  QKQ                 Q+ Q      E               
Sbjct: 145 IQQELEKEKQRVAQQKQQ----------------QLEQEQFHAFEEMI------------ 204

Query: 192 ETLSRHSILGPSGLQGQWKGPSTDKIHYPRNQDFTPEDSRLNQIEQCDLVAHKDSGPGGV 251
                                        RNQ+   E  RL  +++   V   D G GG 
Sbjct: 205 -----------------------------RNQEL--EKERLKIVQEFGKV---DPGLGG- 264

Query: 252 MSMMESVLSLDDGRWPHPADMSTPPLITEVREDPFPLVKQPSPPPVLAKVQQEYATIPPS 311
                          P   D+  P L      D FP +   S  P        + T+ P+
Sbjct: 265 ---------------PLVPDLEKPSL------DVFPTLTVSSIQP-----SDCHTTVRPA 324

Query: 312 K--VADP--RPGVAKQSQDGP--DSFQRLHIPVKMLDDFLRLARENTNKNLETCGVLAGS 371
           K  V D   +PG    S+  P  D  + + +P ++   FL+LA  NT + +ETCG+L G 
Sbjct: 325 KPPVVDRSLKPGALSNSESIPTIDGLRHVVVPGRLCPQFLQLASANTARGVETCGILCGK 384

Query: 372 LKNRVFYISTLIIPKQESTSDSCQTLNEEEIFEVQDRLSLFPLGWIHTHPSQTCFMSSVD 431
           L    F I+ ++IPKQ + SD C T NEEE+F +QD+  L  LGWIHTHP+QT F+SSVD
Sbjct: 385 LMRNEFTITHVLIPKQSAGSDYCNTENEEELFLIQDQQGLITLGWIHTHPTQTAFLSSVD 424

Query: 432 LHTHYSYQIMLPEAIAIVMAPTDASSPYGIFHLSDPGGVSVIRNCQQRGFHPHEEPDDGS 491
           LHTH SYQ+MLPE++AIV +P    +  G F L+D  G+  I +C+Q+GFHPH +     
Sbjct: 445 LHTHCSYQMMLPESVAIVCSPKFQET--GFFKLTD-HGLEEISSCRQKGFHPHSK---DP 424

Query: 492 PLYEHCSHVLMNPNAKFDVMDLR 508
           PL+  CSHV +   A   + DLR
Sbjct: 505 PLFCSCSHVTVVDRA-VTITDLR 424

BLAST of Lag0031552 vs. ExPASy Swiss-Prot
Match: Q9CQ26 (STAM-binding protein OS=Mus musculus OX=10090 GN=Stambp PE=1 SV=1)

HSP 1 Score: 184.9 bits (468), Expect = 2.3e-45
Identity = 151/499 (30.26%), Postives = 222/499 (44.49%), Query Frame = 0

Query: 12  VAVDNRIPLRNYYRIADNLLKQANIYREENNVVDLFIILLRFSSLVSETIPRHRDYQVFF 71
           V ++  IP R YYR    +++ A++Y EE N+   FI+  ++ +L  E +P+HRDY+   
Sbjct: 25  VELNEDIPPRRYYRSGVEIIRMASVYSEEGNIEHAFILYNKYITLFIEKLPKHRDYKSAI 84

Query: 72  PKEKIFYKKKLLSVV-DELESLKPEFDRRVNELEKVYGRPRLPPPDEQERVSYSLRPSSI 131
             EK    KKL SV   + E LK E  RR  +  + Y   +    +E  R          
Sbjct: 85  IPEKKDAVKKLKSVAFPKAEELKTELLRRYTKEYEQYKERKKKEEEELAR---------- 144

Query: 132 ERPYVNGNYSLVLDQKQHANSQPQSSWRYNDDRGQVSQSSSSRIENKFSNLSLNVPLPKP 191
                    ++ + Q+     Q            +V+Q    ++E +  +          
Sbjct: 145 ---------NIAIQQELEKEKQ------------RVAQQKQKQLEQEQFHAF-------E 204

Query: 192 ETLSRHSILGPSGLQGQWKGPSTDKIHYPRNQDFTPEDSRLNQIEQCDLVAHKDSGPGGV 251
           E + R  +                            E  RL  +++   V   D GP G 
Sbjct: 205 EMIQRQEL----------------------------EKERLKIVQEFGKV---DPGPCGP 264

Query: 252 MSMMESVLSLDDGRWPHPADMSTPPLITEVREDPFPLVKQPSPPPVLAKVQQEYATIPPS 311
           +        +D       +   TP   T +R         P+ PPV+ +           
Sbjct: 265 LLPDLEKPCVDVAPSSPFSPTQTPDCNTGMR---------PAKPPVVDR----------- 324

Query: 312 KVADPRPGVAKQSQDGP--DSFQRLHIPVKMLDDFLRLARENTNKNLETCGVLAGSLKNR 371
                +PG     ++ P  +  + + +P  +  +FL+LA  NT K +ETCGVL G L   
Sbjct: 325 ---SLKPGALSVIENVPTIEGLRHIVVPRNLCSEFLQLASANTAKGIETCGVLCGKLMRN 384

Query: 372 VFYISTLIIPKQESTSDSCQTLNEEEIFEVQDRLSLFPLGWIHTHPSQTCFMSSVDLHTH 431
            F I+ ++IP+Q    D C T NEEEIF +QD L L  LGWIHTHP+QT F+SSVDLHTH
Sbjct: 385 EFTITHVLIPRQNGGPDYCHTENEEEIFFMQDDLGLLTLGWIHTHPTQTAFLSSVDLHTH 424

Query: 432 YSYQIMLPEAIAIVMAPTDASSPYGIFHLSDPGGVSVIRNCQQRGFHPHEEPDDGSPLYE 491
            SYQ+MLPE+IAIV +P    +  G F L+D  G+  I  C+Q+GFHPH       PL+ 
Sbjct: 445 CSYQMMLPESIAIVCSPKFQET--GFFKLTD-YGLQEISTCRQKGFHPHGR---DPPLFC 424

Query: 492 HCSHVLMNPNAKFDVMDLR 508
            CSHV +       + DLR
Sbjct: 505 DCSHVTVKDRI-VTITDLR 424

BLAST of Lag0031552 vs. ExPASy TrEMBL
Match: A0A6J1L013 (AMSH-like ubiquitin thioesterase 3 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111499142 PE=3 SV=1)

HSP 1 Score: 964.1 bits (2491), Expect = 2.3e-277
Identity = 479/507 (94.48%), Postives = 489/507 (96.45%), Query Frame = 0

Query: 1   MKINIDREARKVAVDNRIPLRNYYRIADNLLKQANIYREENNVVDLFIILLRFSSLVSET 60
           MKINIDREARKVAVDNRIPLRNY+RIADNLLKQA+IYREENNVVDLFIILLRFSSLVSET
Sbjct: 1   MKINIDREARKVAVDNRIPLRNYFRIADNLLKQADIYREENNVVDLFIILLRFSSLVSET 60

Query: 61  IPRHRDYQVFFPKEKIFYKKKLLSVVDELESLKPEFDRRVNELEKVYGRPRLPPPDEQER 120
           IPRHRDYQ FFPKEKIFYKKKLLSVVDELESLKPEFDRRVNELEKV GRPRLPP DEQ  
Sbjct: 61  IPRHRDYQAFFPKEKIFYKKKLLSVVDELESLKPEFDRRVNELEKVSGRPRLPPSDEQGG 120

Query: 121 VSYSLRPSSIERPYVNGNYSLVLDQKQHANSQPQSSWRYNDDRGQVSQSSSSRIENKFSN 180
           VS SLRPSS+ +PY NGNYSLVLDQKQHANSQPQSSWRY+D RGQVSQSS SRIEN+ SN
Sbjct: 121 VSNSLRPSSMIQPYANGNYSLVLDQKQHANSQPQSSWRYHDGRGQVSQSSYSRIENRLSN 180

Query: 181 LSLNVPLPKPETLSRHSILGPSGLQGQWKGPSTDKIHYPRNQDFTPEDSRLNQIEQCDLV 240
           LSLNVPLPKPETLSRHSILGPSG QGQWKGPSTDKIHYPRNQDFTP DSRLNQIEQCDLV
Sbjct: 181 LSLNVPLPKPETLSRHSILGPSGFQGQWKGPSTDKIHYPRNQDFTPLDSRLNQIEQCDLV 240

Query: 241 AHKDSGPGGVMSMMESVLSLDDGRWPHPADMSTPPLITEVREDPFPLVKQPSPPPVLAKV 300
           AHKDSGPGGVMSMMESVLSLDDGRWPHPAD+S  PLITEVREDPFPLVKQPSPPPVLAKV
Sbjct: 241 AHKDSGPGGVMSMMESVLSLDDGRWPHPADISITPLITEVREDPFPLVKQPSPPPVLAKV 300

Query: 301 QQEYATIPPSKVADPRPGVAKQSQDGPDSFQRLHIPVKMLDDFLRLARENTNKNLETCGV 360
           QQEYATIPPSKVADPRPGVAKQS D  +SFQRLHIPVKMLDDFLRLARENTNKNLETCGV
Sbjct: 301 QQEYATIPPSKVADPRPGVAKQSLDESESFQRLHIPVKMLDDFLRLARENTNKNLETCGV 360

Query: 361 LAGSLKNRVFYISTLIIPKQESTSDSCQTLNEEEIFEVQDRLSLFPLGWIHTHPSQTCFM 420
           LAGSLKNRVFYISTLIIPKQESTSDSCQTLNEEEIFEVQDRLSLFPLGWIHTHPSQTCFM
Sbjct: 361 LAGSLKNRVFYISTLIIPKQESTSDSCQTLNEEEIFEVQDRLSLFPLGWIHTHPSQTCFM 420

Query: 421 SSVDLHTHYSYQIMLPEAIAIVMAPTDASSPYGIFHLSDPGGVSVIRNCQQRGFHPHEEP 480
           SSVDLHTHYSYQIMLPEAIAIVMAPTDASSPYGIFHLSDPGGVSVIRNCQQRGFHPHEEP
Sbjct: 421 SSVDLHTHYSYQIMLPEAIAIVMAPTDASSPYGIFHLSDPGGVSVIRNCQQRGFHPHEEP 480

Query: 481 DDGSPLYEHCSHVLMNPNAKFDVMDLR 508
           +DGSPLYEHCSHVLMNPNAKFDV+DLR
Sbjct: 481 EDGSPLYEHCSHVLMNPNAKFDVIDLR 507

BLAST of Lag0031552 vs. ExPASy TrEMBL
Match: A0A5D3BT78 (AMSH-like ubiquitin thioesterase 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold46G00210 PE=3 SV=1)

HSP 1 Score: 960.7 bits (2482), Expect = 2.5e-276
Identity = 474/510 (92.94%), Postives = 489/510 (95.88%), Query Frame = 0

Query: 1   MKINIDREARKVAVDNRIPLRNYYRIADNLLKQANIYREENNVVDLFIILLRFSSLVSET 60
           MKINIDREARKVAVDNRIPLRNY+RIADNLLKQANIYREENNVVDLFIILLRFSSLVSET
Sbjct: 54  MKINIDREARKVAVDNRIPLRNYFRIADNLLKQANIYREENNVVDLFIILLRFSSLVSET 113

Query: 61  IPRHRDYQVFFPKEKIFYKKKLLSVVDELESLKPEFDRRVNELEKVYGRPRLPPPDEQER 120
           IPRHRDYQ FFPKEKI+YKKKLL+VVDELESLKPEFDRRVNELEKVYGRPRLP PDEQ  
Sbjct: 114 IPRHRDYQAFFPKEKIYYKKKLLNVVDELESLKPEFDRRVNELEKVYGRPRLPLPDEQGS 173

Query: 121 VSYSLRPSSIERPYVNGNYSLVLDQKQHANSQPQSSWRYNDDRGQVSQSSSSRIENKFSN 180
           VS SLRPS  E+PY NGNYSL+LDQKQH NS+PQSSWRY+DDRGQVSQSSS R+ENKFS 
Sbjct: 174 VSNSLRPSPKEQPYANGNYSLMLDQKQHVNSRPQSSWRYSDDRGQVSQSSSLRLENKFST 233

Query: 181 LSLNVPLPKPETLSRHSILGPSGLQGQWKGPSTDKIHYPRNQDFTPEDSRLNQIEQCDLV 240
           +SLNVPLPKPETLSRHSILGPSGLQ QW GPSTDKI YPRNQDFTPEDSRLNQ E CDLV
Sbjct: 234 MSLNVPLPKPETLSRHSILGPSGLQCQWMGPSTDKIQYPRNQDFTPEDSRLNQSEHCDLV 293

Query: 241 AHKDSGPGGVMSMMESVLSLDDGRWPHPADMSTPPLITEVREDPFPLVKQPSPPPVLAKV 300
           AHKDSG GGVMSMMESVLSLDDGRWP+PADMSTPPLITEVREDPFPLVKQPSPPPVLAKV
Sbjct: 294 AHKDSGSGGVMSMMESVLSLDDGRWPNPADMSTPPLITEVREDPFPLVKQPSPPPVLAKV 353

Query: 301 QQEYATIPPSKVADPRPGVAKQSQDGPDSFQRLHIPVKMLDDFLRLARENTNKNLETCGV 360
           QQEYATIPPSKVADPRPGVA+QSQD  +SFQRLHIPVKMLDDFLRLARENTNKNLETCGV
Sbjct: 354 QQEYATIPPSKVADPRPGVARQSQDESESFQRLHIPVKMLDDFLRLARENTNKNLETCGV 413

Query: 361 LAGSLKNRVFYISTLIIPKQESTSDSCQTLNEEEIFEVQDRLSLFPLGWIHTHPSQTCFM 420
           LAGSLKNR+FYISTLIIPKQESTSDSCQTLNEEEIFEVQDRLSLFPLGWIHTHPSQTCFM
Sbjct: 414 LAGSLKNRIFYISTLIIPKQESTSDSCQTLNEEEIFEVQDRLSLFPLGWIHTHPSQTCFM 473

Query: 421 SSVDLHTHYSYQIMLPEAIAIVMAPTDASSPYGIFHLSDPGGVSVIRNCQQRGFHPHEEP 480
           SSVDLHTHYSYQIMLPEAIAIVMAPTD SSPYGIFHLSDPGGVSVIRNCQQRGFHPHEEP
Sbjct: 474 SSVDLHTHYSYQIMLPEAIAIVMAPTDTSSPYGIFHLSDPGGVSVIRNCQQRGFHPHEEP 533

Query: 481 DDGSPLYEHCSHVLMNPNAKFDVMDLRTNG 511
           DDGSPLYEHCSHV+MNPNAKFDV+DLRT G
Sbjct: 534 DDGSPLYEHCSHVMMNPNAKFDVIDLRTIG 563

BLAST of Lag0031552 vs. ExPASy TrEMBL
Match: A0A1S3C8Z8 (LOW QUALITY PROTEIN: AMSH-like ubiquitin thioesterase 3 OS=Cucumis melo OX=3656 GN=LOC103497805 PE=3 SV=1)

HSP 1 Score: 957.6 bits (2474), Expect = 2.1e-275
Identity = 472/507 (93.10%), Postives = 487/507 (96.06%), Query Frame = 0

Query: 1   MKINIDREARKVAVDNRIPLRNYYRIADNLLKQANIYREENNVVDLFIILLRFSSLVSET 60
           MKINIDREARKVAVDNRIPLRNY+RIADNLLKQANIYREENNVVDLFIILLRFSSLVSET
Sbjct: 1   MKINIDREARKVAVDNRIPLRNYFRIADNLLKQANIYREENNVVDLFIILLRFSSLVSET 60

Query: 61  IPRHRDYQVFFPKEKIFYKKKLLSVVDELESLKPEFDRRVNELEKVYGRPRLPPPDEQER 120
           IPRHRDYQ FFPKEKI+YKKKLL+VVDELESLKPEFDRRVNELEKVYGRPRLP PDEQ  
Sbjct: 61  IPRHRDYQAFFPKEKIYYKKKLLNVVDELESLKPEFDRRVNELEKVYGRPRLPLPDEQGS 120

Query: 121 VSYSLRPSSIERPYVNGNYSLVLDQKQHANSQPQSSWRYNDDRGQVSQSSSSRIENKFSN 180
           VS SLRPS  E+PY NGNYSL+LDQKQH NS+PQSSWRY+DDRGQVSQSSS R+ENKFS 
Sbjct: 121 VSNSLRPSPKEQPYANGNYSLMLDQKQHVNSRPQSSWRYSDDRGQVSQSSSLRLENKFST 180

Query: 181 LSLNVPLPKPETLSRHSILGPSGLQGQWKGPSTDKIHYPRNQDFTPEDSRLNQIEQCDLV 240
           +SLNVPLPKPETLSRHSILGPSGLQ QW GPSTDKI YPRNQDFTPEDSRLNQ E CDLV
Sbjct: 181 MSLNVPLPKPETLSRHSILGPSGLQCQWMGPSTDKIQYPRNQDFTPEDSRLNQSEHCDLV 240

Query: 241 AHKDSGPGGVMSMMESVLSLDDGRWPHPADMSTPPLITEVREDPFPLVKQPSPPPVLAKV 300
           AHKDSG GGVMSMMESVLSLDDGRWP+PADMSTPPLITEVREDPFPLVKQPSPPPVLAKV
Sbjct: 241 AHKDSGSGGVMSMMESVLSLDDGRWPNPADMSTPPLITEVREDPFPLVKQPSPPPVLAKV 300

Query: 301 QQEYATIPPSKVADPRPGVAKQSQDGPDSFQRLHIPVKMLDDFLRLARENTNKNLETCGV 360
           QQEYATIPPSKVADPRPGVA+QSQD  +SFQRLHIPVKMLDDFLRLARENTNKNLETCGV
Sbjct: 301 QQEYATIPPSKVADPRPGVARQSQDESESFQRLHIPVKMLDDFLRLARENTNKNLETCGV 360

Query: 361 LAGSLKNRVFYISTLIIPKQESTSDSCQTLNEEEIFEVQDRLSLFPLGWIHTHPSQTCFM 420
           LAGSLKNR+FYISTLIIPKQESTSDSCQTLNEEEIFEVQDRLSLFPLGWIHTHPSQTCFM
Sbjct: 361 LAGSLKNRIFYISTLIIPKQESTSDSCQTLNEEEIFEVQDRLSLFPLGWIHTHPSQTCFM 420

Query: 421 SSVDLHTHYSYQIMLPEAIAIVMAPTDASSPYGIFHLSDPGGVSVIRNCQQRGFHPHEEP 480
           SSVDLHTHYSYQIMLPEAIAIVMAPTD SSPYGIFHLSDPGGVSVIRNCQQRGFHPHEEP
Sbjct: 421 SSVDLHTHYSYQIMLPEAIAIVMAPTDTSSPYGIFHLSDPGGVSVIRNCQQRGFHPHEEP 480

Query: 481 DDGSPLYEHCSHVLMNPNAKFDVMDLR 508
           DDGSPLYEHCSHV+MNPNAKFDV+DLR
Sbjct: 481 DDGSPLYEHCSHVMMNPNAKFDVIDLR 507

BLAST of Lag0031552 vs. ExPASy TrEMBL
Match: A0A6J1H698 (AMSH-like ubiquitin thioesterase 3 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111460414 PE=3 SV=1)

HSP 1 Score: 953.7 bits (2464), Expect = 3.1e-274
Identity = 475/507 (93.69%), Postives = 485/507 (95.66%), Query Frame = 0

Query: 1   MKINIDREARKVAVDNRIPLRNYYRIADNLLKQANIYREENNVVDLFIILLRFSSLVSET 60
           MKINIDREARKVAVDNRIPLRNY+RIADNLLKQA+IYREENNVVDLFIILLRFSSLVSET
Sbjct: 1   MKINIDREARKVAVDNRIPLRNYFRIADNLLKQADIYREENNVVDLFIILLRFSSLVSET 60

Query: 61  IPRHRDYQVFFPKEKIFYKKKLLSVVDELESLKPEFDRRVNELEKVYGRPRLPPPDEQER 120
           IPRHRDYQ FFPKEKIFYKKKLLSVVDELESLKPEFDRRVNELEKV GRPRLPP DEQ  
Sbjct: 61  IPRHRDYQAFFPKEKIFYKKKLLSVVDELESLKPEFDRRVNELEKVSGRPRLPPSDEQRG 120

Query: 121 VSYSLRPSSIERPYVNGNYSLVLDQKQHANSQPQSSWRYNDDRGQVSQSSSSRIENKFSN 180
           VS SLRPSS+ +PY NGNYSLVLDQKQHANSQ QSSWRY+D RGQVSQSS SRIEN+ SN
Sbjct: 121 VSNSLRPSSMIQPYANGNYSLVLDQKQHANSQLQSSWRYHDGRGQVSQSSYSRIENRLSN 180

Query: 181 LSLNVPLPKPETLSRHSILGPSGLQGQWKGPSTDKIHYPRNQDFTPEDSRLNQIEQCDLV 240
           LSLNVPLPKPETLSRHSILGPSG QGQWKGPSTDKIHYPRNQDFTP DSRLNQIEQCDLV
Sbjct: 181 LSLNVPLPKPETLSRHSILGPSGFQGQWKGPSTDKIHYPRNQDFTPFDSRLNQIEQCDLV 240

Query: 241 AHKDSGPGGVMSMMESVLSLDDGRWPHPADMSTPPLITEVREDPFPLVKQPSPPPVLAKV 300
           AHKDSGPGGV+SMMESVLSLDDGRWPHPAD+S  PLITEVREDPFPLVKQPSPPPVLAKV
Sbjct: 241 AHKDSGPGGVVSMMESVLSLDDGRWPHPADISITPLITEVREDPFPLVKQPSPPPVLAKV 300

Query: 301 QQEYATIPPSKVADPRPGVAKQSQDGPDSFQRLHIPVKMLDDFLRLARENTNKNLETCGV 360
           QQEYA IP SKVADPRPGVAKQS D  +SFQRLHIPVKMLDDFLRLARENTNKNLETCGV
Sbjct: 301 QQEYAAIPSSKVADPRPGVAKQSLDESESFQRLHIPVKMLDDFLRLARENTNKNLETCGV 360

Query: 361 LAGSLKNRVFYISTLIIPKQESTSDSCQTLNEEEIFEVQDRLSLFPLGWIHTHPSQTCFM 420
           LAGSLKNRVFYISTLIIPKQESTSDSCQTLNEEEIFEVQDRLSLFPLGWIHTHPSQTCFM
Sbjct: 361 LAGSLKNRVFYISTLIIPKQESTSDSCQTLNEEEIFEVQDRLSLFPLGWIHTHPSQTCFM 420

Query: 421 SSVDLHTHYSYQIMLPEAIAIVMAPTDASSPYGIFHLSDPGGVSVIRNCQQRGFHPHEEP 480
           SSVDLHTHYSYQIMLPEAIAIVMAPTDASSPYGIFHLSDPGGVSVIRNCQQRGFHPHEEP
Sbjct: 421 SSVDLHTHYSYQIMLPEAIAIVMAPTDASSPYGIFHLSDPGGVSVIRNCQQRGFHPHEEP 480

Query: 481 DDGSPLYEHCSHVLMNPNAKFDVMDLR 508
           +DGSPLYEHCSHVLMNPNAKFDV DLR
Sbjct: 481 EDGSPLYEHCSHVLMNPNAKFDVTDLR 507

BLAST of Lag0031552 vs. ExPASy TrEMBL
Match: A0A0A0KG71 (MPN domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G311020 PE=3 SV=1)

HSP 1 Score: 951.8 bits (2459), Expect = 1.2e-273
Identity = 471/507 (92.90%), Postives = 485/507 (95.66%), Query Frame = 0

Query: 1   MKINIDREARKVAVDNRIPLRNYYRIADNLLKQANIYREENNVVDLFIILLRFSSLVSET 60
           MKINIDREARKVAVDNRIPLRNY+RIADNLLKQANIYREENNVVDLFIILLRFSSLVSET
Sbjct: 1   MKINIDREARKVAVDNRIPLRNYFRIADNLLKQANIYREENNVVDLFIILLRFSSLVSET 60

Query: 61  IPRHRDYQVFFPKEKIFYKKKLLSVVDELESLKPEFDRRVNELEKVYGRPRLPPPDEQER 120
           IPRHRDY  FFP EKI+YKKKLL+VVDELESLKPEFDRRVNELEKVYGRPRLPPPDEQ  
Sbjct: 61  IPRHRDYPAFFPNEKIYYKKKLLNVVDELESLKPEFDRRVNELEKVYGRPRLPPPDEQGS 120

Query: 121 VSYSLRPSSIERPYVNGNYSLVLDQKQHANSQPQSSWRYNDDRGQVSQSSSSRIENKFSN 180
           VS S RPS  E+PYVNGNYSL+LDQKQH +S+PQSS RY+DDRGQVS+SSS R+ENKFS 
Sbjct: 121 VSNSFRPSPKEQPYVNGNYSLMLDQKQHVHSRPQSSRRYSDDRGQVSESSSLRLENKFST 180

Query: 181 LSLNVPLPKPETLSRHSILGPSGLQGQWKGPSTDKIHYPRNQDFTPEDSRLNQIEQCDLV 240
           LSLNVPLPKPETLSRHSILGPSGLQGQWKGPSTDKI YP NQDFT EDSRLNQ E CDLV
Sbjct: 181 LSLNVPLPKPETLSRHSILGPSGLQGQWKGPSTDKIQYPINQDFTLEDSRLNQNEHCDLV 240

Query: 241 AHKDSGPGGVMSMMESVLSLDDGRWPHPADMSTPPLITEVREDPFPLVKQPSPPPVLAKV 300
           AHKDSGPGGVMS+MESVLSLDDGRWPHPADMS PPLITEVREDPFPLVKQPSPPPVLAKV
Sbjct: 241 AHKDSGPGGVMSVMESVLSLDDGRWPHPADMSIPPLITEVREDPFPLVKQPSPPPVLAKV 300

Query: 301 QQEYATIPPSKVADPRPGVAKQSQDGPDSFQRLHIPVKMLDDFLRLARENTNKNLETCGV 360
           QQEYATIPPSKVADPRPGVA+QSQD  +SFQRLHIPVKMLDDFLRLARENTNKNLETCGV
Sbjct: 301 QQEYATIPPSKVADPRPGVARQSQDESESFQRLHIPVKMLDDFLRLARENTNKNLETCGV 360

Query: 361 LAGSLKNRVFYISTLIIPKQESTSDSCQTLNEEEIFEVQDRLSLFPLGWIHTHPSQTCFM 420
           LAGSLKNRVFYISTLIIPKQESTSDSCQTLNEEEIFEVQDRLSLFPLGWIHTHPSQTCFM
Sbjct: 361 LAGSLKNRVFYISTLIIPKQESTSDSCQTLNEEEIFEVQDRLSLFPLGWIHTHPSQTCFM 420

Query: 421 SSVDLHTHYSYQIMLPEAIAIVMAPTDASSPYGIFHLSDPGGVSVIRNCQQRGFHPHEEP 480
           SSVDLHTHYSYQIMLPEAIAIVMAPTD SSPYGIFHLSDPGGVSVIRNCQQRGFHPHEEP
Sbjct: 421 SSVDLHTHYSYQIMLPEAIAIVMAPTDTSSPYGIFHLSDPGGVSVIRNCQQRGFHPHEEP 480

Query: 481 DDGSPLYEHCSHVLMNPNAKFDVMDLR 508
           DDGSPLYEHCSHV+MNPNAKFDVMDLR
Sbjct: 481 DDGSPLYEHCSHVMMNPNAKFDVMDLR 507

BLAST of Lag0031552 vs. TAIR 10
Match: AT4G16144.1 (associated molecule with the SH3 domain of STAM 3 )

HSP 1 Score: 579.3 bits (1492), Expect = 3.0e-165
Identity = 310/515 (60.19%), Postives = 388/515 (75.34%), Query Frame = 0

Query: 1   MKINIDREARKVAVDNRIPLRNYYRIADNLLKQANIYREENNVVDLFIILLRFSSLVSET 60
           MKI++++ AR++ VDNRIPLRNYYRIADNLL+QA+IYREE NVVDL+I+LLR+SSL+SET
Sbjct: 1   MKIDLNKVAREIEVDNRIPLRNYYRIADNLLRQASIYREEKNVVDLYIMLLRYSSLISET 60

Query: 61  IPRHRDYQVFFPKEKIFYKKKLLSVVDELESLKPEFDRRVNELEKVYGRPRLPPPDEQER 120
           IP HRDYQ   P+E++  +K+L +V++ELESLKPEF++ V++L +V    R    D    
Sbjct: 61  IPFHRDYQASLPQERLGSRKRLRAVINELESLKPEFNQLVDKLNRVEDESRQDGSD-LPV 120

Query: 121 VSYSLRPSSIERPYVN-GNYSLVLDQKQHANSQPQSSWRYNDDRGQVSQSSSSRIENKFS 180
           VSYS    ++E P  +  +YS     K    SQP  SW YN++    S S+ ++I+ +F 
Sbjct: 121 VSYS--SDAVEWPPAHKASYSRPDINKPLPTSQP--SWTYNNN--LTSSSNRTQIDQQFQ 180

Query: 181 NLSLNVPLPKPETLSRHSILGPSGLQGQWKGPSTD-KIHYPRNQDF-TPEDSRLNQIEQC 240
            LS +   P   TLSRHS LGP+GL+ Q   P ++ K+ YP N D+ + ++S L +    
Sbjct: 181 KLSFDFLPPNQATLSRHSFLGPNGLKRQMVAPKSEIKVQYPSNTDWGSADNSGLIEAGPS 240

Query: 241 DLVAHKDSGPGGVMSMMESVLSLDDGRWPHPADMSTPPLITEVREDPFPLV--KQPSPPP 300
              A   +G    +S + SVLSLDDGRW   ++      I++  EDPF  V  KQPSPPP
Sbjct: 241 SSSASL-NGDSQEVSTLNSVLSLDDGRWQRHSEAVNSQFISDATEDPFQFVGMKQPSPPP 300

Query: 301 VLAKVQQEYATIPPSKVADPRPGVA---KQSQDGPDSFQRLHIPVKMLDDFLRLARENTN 360
           VLA+V QE A I PSKVADPRPG A    + ++G +S+Q LH+PV+++DDFLRLAR NT 
Sbjct: 301 VLAQVHQELAQICPSKVADPRPGPAIPSLEGKEGSNSYQHLHVPVRIMDDFLRLARSNTE 360

Query: 361 KNLETCGVLAGSLKNRVFYISTLIIPKQESTSDSCQTLNEEEIFEVQDRLSLFPLGWIHT 420
           +NLETCGVLAGSLKNRVF+I+TLIIPKQESTSDSCQTLNEEEIFEVQDRLSLFPLGWIHT
Sbjct: 361 RNLETCGVLAGSLKNRVFHITTLIIPKQESTSDSCQTLNEEEIFEVQDRLSLFPLGWIHT 420

Query: 421 HPSQTCFMSSVDLHTHYSYQIMLPEAIAIVMAPTDASSPYGIFHLSDPGGVSVIRNCQQR 480
           HP+QTCFMSSVDLHTHYSYQIMLPEA+AIVMAPTD S+P+GIFHLSDP GVSVIRNCQQR
Sbjct: 421 HPTQTCFMSSVDLHTHYSYQIMLPEAVAIVMAPTDESTPHGIFHLSDPSGVSVIRNCQQR 480

Query: 481 GFHPHEEPDDGSPLYEHCSHVLMNPNAKFDVMDLR 508
           GFHPHEE +DG+P+YEHCSHV +N   K++V+DLR
Sbjct: 481 GFHPHEESEDGNPIYEHCSHVFLNAKLKYEVLDLR 507

BLAST of Lag0031552 vs. TAIR 10
Match: AT1G48790.1 (associated molecule with the SH3 domain of STAM 1 )

HSP 1 Score: 435.6 bits (1119), Expect = 5.4e-122
Identity = 249/514 (48.44%), Postives = 327/514 (63.62%), Query Frame = 0

Query: 3   INIDREARKVAVDNRIPLRNYYRIADNLLKQANIYREENNVVDLFIILLRFSSLVSETIP 62
           I+I   AR++ VDNRI L+ Y+RIADN+LKQANI+R E NV+DL+++LLRFSSL  ETIP
Sbjct: 8   IDIATSARRIGVDNRISLKFYFRIADNILKQANIFRAEKNVIDLYVMLLRFSSLALETIP 67

Query: 63  RHRDYQVFFPKEKIFYKKKLLSVVDELESLKPEFDRRVNELEKVYGRPRLPP--PDEQER 122
            HRDY+      K + + +LL V+ ELE LKP   +R++EL      P+L P    +   
Sbjct: 68  SHRDYRTSLKSNKEYLRMRLLDVLTELEKLKPVVQQRIDEL-----YPKLKPRYNVQAHP 127

Query: 123 VSYSLRPSSIERPYVNGNYSLVLDQKQHANSQPQSSWRYNDDRGQVSQSSSSRIENKFSN 182
            + SL  SS  +P  N              + P  +  Y   RGQ    +++ +E +F  
Sbjct: 128 ANGSLGWSSAVKPSFNSY------DHAKVRNPPGHNSGYMGSRGQ-QFLNAAPLEERFRK 187

Query: 183 LSLNVPLPKPETLSRHSILGPSGLQGQWKGPSTD-KIHYPRNQDFTPE--DSRLNQIEQC 242
           +S+N   P  ETLS+HSILGP GL  QW+ P  D K+ YP N DF+P    S    ++  
Sbjct: 188 MSVNF-RPNEETLSKHSILGPGGLSAQWQPPKYDTKVQYPSNIDFSPVVIPSFQQLVDSK 247

Query: 243 DLVAHKDSGPGGVMSMMESVLSLDDGRWPHPADMSTPPLITEVRE-DPFPLVKQPSPPPV 302
            ++ +  +       +  SV S +  +  +  ++S+     E    +   L++QPSPPPV
Sbjct: 248 PMITNGSNDEPEKPIVEPSVASNEKIQKNYTEELSSMISFEEPESVNENNLIRQPSPPPV 307

Query: 303 LAKVQQEYATIPPSKVADPRPGVAKQSQD---GPDSFQRLHIPVKMLDDFLRLARENTNK 362
           LA+VQ     + P +V +P   +     D     +S   LHI   M+D F+RLA+ NT K
Sbjct: 308 LAEVQDLVPALCP-EVREPECMIENSLPDESLRSESPLELHIATSMMDTFMRLAKSNTKK 367

Query: 363 NLETCGVLAGSLKNRVFYISTLIIPKQESTSDSCQTLNEEEIFEVQDRLSLFPLGWIHTH 422
           NLETCG+LAGSLKNR FYI+ LIIPKQESTSDSCQ  NEEEIFEVQD+ SLFPLGWIHTH
Sbjct: 368 NLETCGILAGSLKNRKFYITALIIPKQESTSDSCQATNEEEIFEVQDKQSLFPLGWIHTH 427

Query: 423 PSQTCFMSSVDLHTHYSYQIMLPEAIAIVMAPTDASSPYGIFHLSDPGGVSVIRNCQQRG 482
           P+Q+CFMSS+D+HTHYSYQIMLPEA+AIVMAP D+S  +GIF L+ PGG++VIRNC +RG
Sbjct: 428 PTQSCFMSSIDVHTHYSYQIMLPEAVAIVMAPQDSSRNHGIFRLTTPGGMTVIRNCDRRG 487

Query: 483 FHPHEEPDDGSPLYEHCSHVLMNPNAKFDVMDLR 508
           FH H  P+DG P+Y  C  V MNPN KFDV+DLR
Sbjct: 488 FHAHSSPEDGGPIYNTCKEVYMNPNLKFDVIDLR 507

BLAST of Lag0031552 vs. TAIR 10
Match: AT1G10600.1 (associated molecule with the SH3 domain of STAM 2 )

HSP 1 Score: 265.4 bits (677), Expect = 9.6e-71
Identity = 113/175 (64.57%), Postives = 145/175 (82.86%), Query Frame = 0

Query: 333 LHIPVKMLDDFLRLARENTNKNLETCGVLAGSLKNRVFYISTLIIPKQESTSDSCQTLNE 392
           +HI  ++L+DF  LARENT K+LETCG LA  L+  +FY++TLIIPKQESTS+SCQ +NE
Sbjct: 49  VHISERLLEDFTELARENTEKDLETCGTLAAFLERGIFYVTTLIIPKQESTSNSCQAMNE 108

Query: 393 EEIFEVQDRLSLFPLGWIHTHPSQTCFMSSVDLHTHYSYQIMLPEAIAIVMAPTDASSPY 452
            E+F +Q+   L+P+GWIHTHPSQ CFMSSVDLHTHYSYQ+M+PEA AIV+APTD+S  Y
Sbjct: 109 VEVFSIQNERELYPVGWIHTHPSQGCFMSSVDLHTHYSYQVMVPEAFAIVVAPTDSSKSY 168

Query: 453 GIFHLSDPGGVSVIRNCQQRGFHPHEEPDDGSPLYEHCSHVLMNPNAKFDVMDLR 508
           GIF L+DPGG+ V+R C + GFHPH+EP+DG+P+YEHCS+V  N N +F++ DLR
Sbjct: 169 GIFKLTDPGGMEVLRGCSETGFHPHKEPEDGNPVYEHCSNVYKNSNLRFEIFDLR 223

BLAST of Lag0031552 vs. TAIR 10
Match: AT1G10600.3 (associated molecule with the SH3 domain of STAM 2 )

HSP 1 Score: 261.5 bits (667), Expect = 1.4e-69
Identity = 114/175 (65.14%), Postives = 145/175 (82.86%), Query Frame = 0

Query: 333 LHIPVKMLDDFLRLARENTNKNLETCGVLAGSLKNRVFYISTLIIPKQESTSDSCQTLNE 392
           +HI  ++L+DF  LARENT K+LETCG LA  L+  +FY++TLIIPKQESTS+SCQ +NE
Sbjct: 49  VHISERLLEDFTELARENTEKDLETCGTLAAFLERGIFYVTTLIIPKQESTSNSCQAMNE 108

Query: 393 EEIFEVQDRLSLFPLGWIHTHPSQTCFMSSVDLHTHYSYQIMLPEAIAIVMAPTDASSPY 452
            E+F +Q+   L+P+GWIHTHPSQ CFMSSVDLHTHYSYQ+M+PEA AIV+APTD SS Y
Sbjct: 109 VEVFSIQNERELYPVGWIHTHPSQGCFMSSVDLHTHYSYQVMVPEAFAIVVAPTD-SSNY 168

Query: 453 GIFHLSDPGGVSVIRNCQQRGFHPHEEPDDGSPLYEHCSHVLMNPNAKFDVMDLR 508
           GIF L+DPGG+ V+R C + GFHPH+EP+DG+P+YEHCS+V  N N +F++ DLR
Sbjct: 169 GIFKLTDPGGMEVLRGCSETGFHPHKEPEDGNPVYEHCSNVYKNSNLRFEIFDLR 222

BLAST of Lag0031552 vs. TAIR 10
Match: AT1G10600.2 (associated molecule with the SH3 domain of STAM 2 )

HSP 1 Score: 194.1 bits (492), Expect = 2.7e-49
Identity = 79/121 (65.29%), Postives = 101/121 (83.47%), Query Frame = 0

Query: 387 CQTLNEEEIFEVQDRLSLFPLGWIHTHPSQTCFMSSVDLHTHYSYQIMLPEAIAIVMAPT 446
           CQ +NE E+F +Q+   L+P+GWIHTHPSQ CFMSSVDLHTHYSYQ+M+PEA AIV+APT
Sbjct: 46  CQAMNEVEVFSIQNERELYPVGWIHTHPSQGCFMSSVDLHTHYSYQVMVPEAFAIVVAPT 105

Query: 447 DASSPYGIFHLSDPGGVSVIRNCQQRGFHPHEEPDDGSPLYEHCSHVLMNPNAKFDVMDL 506
           D+S  YGIF L+DPGG+ V+R C + GFHPH+EP+DG+P+YEHCS+V  N N +F++ DL
Sbjct: 106 DSSKSYGIFKLTDPGGMEVLRGCSETGFHPHKEPEDGNPVYEHCSNVYKNSNLRFEIFDL 165

Query: 507 R 508
           R
Sbjct: 166 R 166

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038875747.11.4e-28195.27AMSH-like ubiquitin thioesterase 3 [Benincasa hispida][more]
XP_023006405.14.7e-27794.48AMSH-like ubiquitin thioesterase 3 isoform X1 [Cucurbita maxima][more]
TYK02941.15.2e-27692.94AMSH-like ubiquitin thioesterase 3 [Cucumis melo var. makuwa][more]
XP_008458380.14.4e-27593.10PREDICTED: LOW QUALITY PROTEIN: AMSH-like ubiquitin thioesterase 3 [Cucumis melo... [more]
XP_023549197.14.8e-27493.49AMSH-like ubiquitin thioesterase 3 isoform X1 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q5PNU34.2e-16460.19AMSH-like ubiquitin thioesterase 3 OS=Arabidopsis thaliana OX=3702 GN=AMSH3 PE=1... [more]
Q8VYB57.6e-12148.44AMSH-like ubiquitin thioesterase 1 OS=Arabidopsis thaliana OX=3702 GN=AMSH1 PE=2... [more]
Q6NKP91.4e-6964.57AMSH-like ubiquitin thioesterase 2 OS=Arabidopsis thaliana OX=3702 GN=AMSH2 PE=2... [more]
O956305.6e-4731.01STAM-binding protein OS=Homo sapiens OX=9606 GN=STAMBP PE=1 SV=1[more]
Q9CQ262.3e-4530.26STAM-binding protein OS=Mus musculus OX=10090 GN=Stambp PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1L0132.3e-27794.48AMSH-like ubiquitin thioesterase 3 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC... [more]
A0A5D3BT782.5e-27692.94AMSH-like ubiquitin thioesterase 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A1S3C8Z82.1e-27593.10LOW QUALITY PROTEIN: AMSH-like ubiquitin thioesterase 3 OS=Cucumis melo OX=3656 ... [more]
A0A6J1H6983.1e-27493.69AMSH-like ubiquitin thioesterase 3 isoform X1 OS=Cucurbita moschata OX=3662 GN=L... [more]
A0A0A0KG711.2e-27392.90MPN domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G311020 PE=3 SV... [more]
Match NameE-valueIdentityDescription
AT4G16144.13.0e-16560.19associated molecule with the SH3 domain of STAM 3 [more]
AT1G48790.15.4e-12248.44associated molecule with the SH3 domain of STAM 1 [more]
AT1G10600.19.6e-7164.57associated molecule with the SH3 domain of STAM 2 [more]
AT1G10600.31.4e-6965.14associated molecule with the SH3 domain of STAM 2 [more]
AT1G10600.22.7e-4965.29associated molecule with the SH3 domain of STAM 2 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 86..106
NoneNo IPR availableGENE3D1.20.58.80coord: 3..120
e-value: 7.5E-33
score: 115.1
NoneNo IPR availableGENE3D3.40.140.10Cytidine Deaminase, domain 2coord: 318..509
e-value: 2.0E-71
score: 241.3
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 146..174
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 309..330
NoneNo IPR availablePANTHERPTHR12947AMSH-LIKE PROTEASEcoord: 2..507
NoneNo IPR availablePANTHERPTHR12947:SF18AMSH-LIKE UBIQUITIN THIOESTERASE 3coord: 2..507
NoneNo IPR availableSUPERFAMILY102712JAB1/MPN domaincoord: 332..463
IPR000555JAB1/MPN/MOV34 metalloenzyme domainSMARTSM00232pad1_6coord: 332..460
e-value: 2.0E-13
score: 60.6
IPR000555JAB1/MPN/MOV34 metalloenzyme domainPFAMPF01398JABcoord: 329..436
e-value: 2.2E-16
score: 59.9
IPR015063USP8 dimerisation domainPFAMPF08969USP8_dimercoord: 8..99
e-value: 1.5E-11
score: 44.4
IPR037518MPN domainPROSITEPS50249MPNcoord: 333..463
score: 30.546385
IPR044098STAMBP/STALP-like, MPN domainCDDcd08066MPN_AMSH_likecoord: 333..507
e-value: 2.58146E-115
score: 336.104

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0031552.1Lag0031552.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006897 endocytosis
biological_process GO:0090316 positive regulation of intracellular protein transport
biological_process GO:0044090 positive regulation of vacuole organization
biological_process GO:0071108 protein K48-linked deubiquitination
biological_process GO:0070536 protein K63-linked deubiquitination
biological_process GO:0016579 protein deubiquitination
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005770 late endosome
molecular_function GO:0070122 isopeptidase activity
molecular_function GO:0061578 Lys63-specific deubiquitinase activity
molecular_function GO:0008237 metallopeptidase activity
molecular_function GO:0005515 protein binding
molecular_function GO:0004843 thiol-dependent deubiquitinase