Lag0031534 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0031534
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionF-box protein CPR30-like
Locationchr11: 9687015 .. 9688130 (-)
RNA-Seq ExpressionLag0031534
SyntenyLag0031534
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGAATCTGGAAGATCTGCCGGAAGAAGTGATGATGATGATTCTGTTGCGGGTGCCGCCGGAGTCTCTGGCTCGATTCAAGTTGGTTGCGAAATCCTGGTACGCCCTCATTAATGACCCTAAATTCCTCGATTCTCTTCCGAACCCAAACGTTTTACTGAAGCAACTTGTGAAGAACAAGGAAACTGGGAAGAAGGCAATTAGATTCTCCTTCTTGAAATATCGCTTCGTAGTTGATGATTTTGTCGTTGAAGACGTCCATATTCCATTTCCTGGTAAGGAATTTCATTACCACACCCTCGTCCACGGCCATTCTCACGGCTTAGTTTGCCTCTCTGGTGGCCACGACGGTATTTGCCTCTATAAACCCGCCACCAGAGAATTCCGCATCCTCCCGCCCTCGATTATTCACATCAATCGACCCTACAATGTAAACGATGTCAATTCGTGGACCAACGCAGTGGGATTCGGGTACGATCCAAAATCTAAGGATTTCAAGGTGGTTAGGGTTGTTGATTTTACAGGACCGTTTTCGAAATTTTTGCGAGCTGAAGTGTTGGATTTGGAGAAAAATGAATGGAGAGAAATCGAAACCCCTATCCCTGGGTGTCGTGACTATCAGCCTTCGTTTGAGATGTATTACCAAGGATTCTATTATTGGTGGGCGCTAGATAGAATAGAATCTGGATTTATAATGTCATTCGACATGAGCGAAGAGGTTTTCAGGATGATTCCAGCGCCGGAGAGTTTCCAAATGTACCATTACAAAAGCATGGGGGTTTTGAATGGATCCATCGTTCTCTTTTATTATCCCTGGGGTGCGGATAAAAAGAGTTTCGAGGTTTGGAAGATGGTGAAGGATGATGAATTGGTTTCATGGTCAAAGATGTTCACATTTAGCCCAGATTCTGGAGTTGAGTTCCCTGTAGCCTTTGTGGGCTCTCATCAACTACTAATGGAGACCAACGAGGGACAGCTCATTTTGTACAACGTTAACACCCAACGCTTCAAACATCTCCCACTACATGGAGGCCTTCCAAGTAGATCCCAAGCTACAGTTTTCATCCAGACTTTGTCCTCTGTCAATCTCAATAGAGGACATAACCATAATTAA

mRNA sequence

ATGGCGAATCTGGAAGATCTGCCGGAAGAAGTGATGATGATGATTCTGTTGCGGGTGCCGCCGGAGTCTCTGGCTCGATTCAAGTTGGTTGCGAAATCCTGGTACGCCCTCATTAATGACCCTAAATTCCTCGATTCTCTTCCGAACCCAAACGTTTTACTGAAGCAACTTGTGAAGAACAAGGAAACTGGGAAGAAGGCAATTAGATTCTCCTTCTTGAAATATCGCTTCGTAGTTGATGATTTTGTCGTTGAAGACGTCCATATTCCATTTCCTGGTAAGGAATTTCATTACCACACCCTCGTCCACGGCCATTCTCACGGCTTAGTTTGCCTCTCTGGTGGCCACGACGGTATTTGCCTCTATAAACCCGCCACCAGAGAATTCCGCATCCTCCCGCCCTCGATTATTCACATCAATCGACCCTACAATGTAAACGATGTCAATTCGTGGACCAACGCAGTGGGATTCGGGTACGATCCAAAATCTAAGGATTTCAAGGTGGTTAGGGTTGTTGATTTTACAGGACCGTTTTCGAAATTTTTGCGAGCTGAAGTGTTGGATTTGGAGAAAAATGAATGGAGAGAAATCGAAACCCCTATCCCTGGGTGTCGTGACTATCAGCCTTCGTTTGAGATGTATTACCAAGGATTCTATTATTGGTGGGCGCTAGATAGAATAGAATCTGGATTTATAATGTCATTCGACATGAGCGAAGAGGTTTTCAGGATGATTCCAGCGCCGGAGAGTTTCCAAATGTACCATTACAAAAGCATGGGGGTTTTGAATGGATCCATCGTTCTCTTTTATTATCCCTGGGGTGCGGATAAAAAGAGTTTCGAGGTTTGGAAGATGGTGAAGGATGATGAATTGGTTTCATGGTCAAAGATGTTCACATTTAGCCCAGATTCTGGAGTTGAGTTCCCTGTAGCCTTTGTGGGCTCTCATCAACTACTAATGGAGACCAACGAGGGACAGCTCATTTTGTACAACGTTAACACCCAACGCTTCAAACATCTCCCACTACATGGAGGCCTTCCAAGTAGATCCCAAGCTACAGTTTTCATCCAGACTTTGTCCTCTGTCAATCTCAATAGAGGACATAACCATAATTAA

Coding sequence (CDS)

ATGGCGAATCTGGAAGATCTGCCGGAAGAAGTGATGATGATGATTCTGTTGCGGGTGCCGCCGGAGTCTCTGGCTCGATTCAAGTTGGTTGCGAAATCCTGGTACGCCCTCATTAATGACCCTAAATTCCTCGATTCTCTTCCGAACCCAAACGTTTTACTGAAGCAACTTGTGAAGAACAAGGAAACTGGGAAGAAGGCAATTAGATTCTCCTTCTTGAAATATCGCTTCGTAGTTGATGATTTTGTCGTTGAAGACGTCCATATTCCATTTCCTGGTAAGGAATTTCATTACCACACCCTCGTCCACGGCCATTCTCACGGCTTAGTTTGCCTCTCTGGTGGCCACGACGGTATTTGCCTCTATAAACCCGCCACCAGAGAATTCCGCATCCTCCCGCCCTCGATTATTCACATCAATCGACCCTACAATGTAAACGATGTCAATTCGTGGACCAACGCAGTGGGATTCGGGTACGATCCAAAATCTAAGGATTTCAAGGTGGTTAGGGTTGTTGATTTTACAGGACCGTTTTCGAAATTTTTGCGAGCTGAAGTGTTGGATTTGGAGAAAAATGAATGGAGAGAAATCGAAACCCCTATCCCTGGGTGTCGTGACTATCAGCCTTCGTTTGAGATGTATTACCAAGGATTCTATTATTGGTGGGCGCTAGATAGAATAGAATCTGGATTTATAATGTCATTCGACATGAGCGAAGAGGTTTTCAGGATGATTCCAGCGCCGGAGAGTTTCCAAATGTACCATTACAAAAGCATGGGGGTTTTGAATGGATCCATCGTTCTCTTTTATTATCCCTGGGGTGCGGATAAAAAGAGTTTCGAGGTTTGGAAGATGGTGAAGGATGATGAATTGGTTTCATGGTCAAAGATGTTCACATTTAGCCCAGATTCTGGAGTTGAGTTCCCTGTAGCCTTTGTGGGCTCTCATCAACTACTAATGGAGACCAACGAGGGACAGCTCATTTTGTACAACGTTAACACCCAACGCTTCAAACATCTCCCACTACATGGAGGCCTTCCAAGTAGATCCCAAGCTACAGTTTTCATCCAGACTTTGTCCTCTGTCAATCTCAATAGAGGACATAACCATAATTAA

Protein sequence

MANLEDLPEEVMMMILLRVPPESLARFKLVAKSWYALINDPKFLDSLPNPNVLLKQLVKNKETGKKAIRFSFLKYRFVVDDFVVEDVHIPFPGKEFHYHTLVHGHSHGLVCLSGGHDGICLYKPATREFRILPPSIIHINRPYNVNDVNSWTNAVGFGYDPKSKDFKVVRVVDFTGPFSKFLRAEVLDLEKNEWREIETPIPGCRDYQPSFEMYYQGFYYWWALDRIESGFIMSFDMSEEVFRMIPAPESFQMYHYKSMGVLNGSIVLFYYPWGADKKSFEVWKMVKDDELVSWSKMFTFSPDSGVEFPVAFVGSHQLLMETNEGQLILYNVNTQRFKHLPLHGGLPSRSQATVFIQTLSSVNLNRGHNHN
Homology
BLAST of Lag0031534 vs. NCBI nr
Match: XP_023526117.1 (F-box protein CPR1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 358.2 bits (918), Expect = 8.4e-95
Identity = 206/382 (53.93%), Postives = 263/382 (68.85%), Query Frame = 0

Query: 1   MANLEDLPEEVMMMILLRVPPESLARFKLVAKSWYALINDPKFL-----DSLPNPNVLLK 60
           MA L  LPEEVM+ IL R+PPESL RFK V KSWYALI++PKF      +SL   +VLLK
Sbjct: 1   MAALGHLPEEVMIEILSRLPPESLLRFKTVRKSWYALIDNPKFATKHFSNSLKQKHVLLK 60

Query: 61  QLVKNKETGKKAIRFSFLKYRFVVDDFV-VEDVHIPFPGKEFHYHTLVHGHSHGLVCLSG 120
           +LV  K +GKK   FSFLK+   +D  V V DV +PF  ++F Y   + GHSHGL+CL+ 
Sbjct: 61  RLV-TKNSGKKENIFSFLKFPLPLDGSVPVYDVDLPF-DEDFRYFE-IRGHSHGLLCLTD 120

Query: 121 GHDGICLYKPATREFRILPPSIIHINRPYNVND-VNSWTNAVGFGYDPKSKDFKVVRVVD 180
               I L  PATREFR LPPSI+ + +P    D  +S TNAVGFGYD KS+DFKVVRVV+
Sbjct: 121 LRRDIFLCNPATREFRKLPPSILLLTKPPTEPDGYDSSTNAVGFGYDSKSRDFKVVRVVN 180

Query: 181 FT-GP-FSKFLRAEVLDLEKNEWREIETPIPGCRDYQPSFEMYYQGFYYWWALDRIESGF 240
           F  GP +    R E+ DL K+ WREIETPI G   + PSFEMY++G YYWWA        
Sbjct: 181 FVEGPGYFHLPRVEIYDLSKDRWREIETPICGHVFWAPSFEMYHEGTYYWWADTDEGVEI 240

Query: 241 IMSFDMSEEVFRMIPAPESFQ--MYHYKSMGVLNGSIVLFYYPWGADKKSFEVWKMVKDD 300
           I SFDM+EEVFR I  PESF+     Y+SMGVLNGSIVLF+YP   D++ F++W++VKD+
Sbjct: 241 IQSFDMTEEVFRRISVPESFEGKRERYRSMGVLNGSIVLFHYPSRGDEREFDIWEIVKDE 300

Query: 301 -ELVSWSKMFTFSPDSGVEFPVAFVGSHQLLMETNEGQLILYNVNTQRFKHLPLHGGLPS 360
              VSWSK+ T  P  G+E P+ FV S +LLME NEG+++LYN  TQ++K LP++G  P+
Sbjct: 301 FGGVSWSKLLTIGPLGGIEKPLVFVSSDELLMEANEGRMVLYNARTQQWKELPINGH-PA 360

Query: 361 RSQATVFIQTLSSVNLNRGHNH 371
           R QAT+F+++L SV   +G N+
Sbjct: 361 RFQATLFVKSLVSV---KGRNN 375

BLAST of Lag0031534 vs. NCBI nr
Match: XP_022983805.1 (F-box protein CPR1-like [Cucurbita maxima])

HSP 1 Score: 357.5 bits (916), Expect = 1.4e-94
Identity = 207/375 (55.20%), Postives = 256/375 (68.27%), Query Frame = 0

Query: 1   MANLEDLPEEVMMMILLRVPPESLARFKLVAKSWYALINDPKFL-----DSLPNPNVLLK 60
           MA L  LPEEVM+ IL R+PPESL RFK V KSWYALI++PKF      +SL + +VLLK
Sbjct: 1   MAALGHLPEEVMIEILSRLPPESLLRFKTVRKSWYALIDNPKFATKHFSNSLKHKHVLLK 60

Query: 61  QLVKNKETGKKAIRFSFLKYRFVVDDFV-VEDVHIPFPGKEFHYHTLVHGHSHGLVCLSG 120
           +LV  K +GKK   FSFLK+   +D  V V DV  PF  ++F Y   + GHSHGL+CL+ 
Sbjct: 61  RLV-TKNSGKKENIFSFLKFPLPLDGSVPVFDVDWPF-DEDFRYFE-IRGHSHGLLCLTD 120

Query: 121 GHDGICLYKPATREFRILPPSIIHINRPYNVND-VNSWTNAVGFGYDPKSKDFKVVRVVD 180
               I L  PATREFR LP SI+ +  P    D  +S TNAVGFGYD KSKDFKVVRVV+
Sbjct: 121 LRRDIFLCNPATREFRKLPQSILLLTEPPTEPDGYDSSTNAVGFGYDSKSKDFKVVRVVN 180

Query: 181 FT-GP-FSKFLRAEVLDLEKNEWREIETPIPGCRDYQPSFEMYYQGFYYWWALDRIESGF 240
           F  GP +    RAE+ DL K+ WREIETPI G   + PSFEMY++G YYWWA    +   
Sbjct: 181 FVEGPGYFHPPRAEIYDLSKDRWREIETPICGHVFWAPSFEMYHEGTYYWWADTDDKEEI 240

Query: 241 IMSFDMSEEVFRMIPAPESF--QMYHYKSMGVLNGSIVLFYYPWGADKKSFEVWKMVKDD 300
           I SFDMSEEVFR IP PESF  +   Y+SMG+LNGSIVLF+YP   D++ F++W M KD+
Sbjct: 241 IQSFDMSEEVFRRIPVPESFEGERERYRSMGILNGSIVLFHYPSRGDEREFDIWGMEKDE 300

Query: 301 -ELVSWSKMFTFSPDSGVEFPVAFVGSHQLLMETNEGQLILYNVNTQRFKHLPLHGGLPS 360
              VSWSK+ T  P  G+E P+ FV S +LLME NEGQ++LYN   Q+ K LP+ G  P+
Sbjct: 301 FGGVSWSKLLTIGPLRGIEKPLVFVSSDELLMEANEGQIVLYNARIQQLKELPIKGH-PA 360

Query: 361 RSQATVFIQTLSSVN 364
           R QAT+F+++L SVN
Sbjct: 361 RFQATLFVKSLVSVN 371

BLAST of Lag0031534 vs. NCBI nr
Match: XP_038906036.1 (F-box protein CPR1-like [Benincasa hispida])

HSP 1 Score: 354.8 bits (909), Expect = 9.3e-94
Identity = 202/374 (54.01%), Postives = 256/374 (68.45%), Query Frame = 0

Query: 1   MANLEDLPEEVMMMILLRVPPESLARFKLVAKSWYALINDPK-----FLDSLPNPNVLLK 60
           MA+L +LPEEVM+ IL R+PPESL RFK V KSWYALINDPK     F +SL +  VLLK
Sbjct: 1   MASLGNLPEEVMIEILSRLPPESLLRFKCVRKSWYALINDPKFKAKHFSNSLQHKQVLLK 60

Query: 61  QLVKNKETGKKAIRFSFLKYRFVVDDFV-VEDVHIPFPGKEFHYHTLVHGHSHGLVCLSG 120
           +LV  K +G K   FSFLK    +D  V V D+ +PF  ++F +   +HGHSHGL+CL+ 
Sbjct: 61  RLV-TKNSGNKENIFSFLKLPLSIDRSVSVSDLDLPF-DEDFRFFE-IHGHSHGLLCLTD 120

Query: 121 GHDGICLYKPATREFRILPPSIIHINRPYNVND-VNSWTNAVGFGYDPKSKDFKVVRVVD 180
               I L  P+TREF  LPPSI+ +  P    D  +S TNAVGFGYD KS+DFKVVRVVD
Sbjct: 121 LRKDIFLCNPSTREFHKLPPSILLLTEPPAEPDGYDSSTNAVGFGYDSKSRDFKVVRVVD 180

Query: 181 FT-GP-FSKFLRAEVLDLEKNEWREIETPIPGCRDYQPSFEMYYQGFYYWWALDRIESGF 240
           F  GP +    R E+ DL K+ WREIETP+ G   + P FEMY++G YYWWA     +  
Sbjct: 181 FVEGPGYFHPPRVEIYDLSKDRWREIETPVCGHVFWAPCFEMYHEGTYYWWAETEGGTEI 240

Query: 241 IMSFDMSEEVFRMIPAPESFQMY--HYKSMGVLNGSIVLFYYPWGADKKSFEVWKMVKDD 300
           I +FDMSEEVF  I  PESFQ     Y+S+GVLNGSIVLF+YP   D++SF++W+M KD+
Sbjct: 241 IQTFDMSEEVFGRILVPESFQGTGDRYRSLGVLNGSIVLFHYPSRGDERSFDIWEMEKDE 300

Query: 301 E-LVSWSKMFTFSPDSGVEFPVAFVGSHQLLMETNEGQLILYNVNTQRFKHLPLHGGLPS 360
              VSWSK+ T  P SG+E P+ FV S +LLME +EGQ+ILYN+ TQ+ K +P+ G  P+
Sbjct: 301 SGGVSWSKLLTIGPVSGIEKPLLFVSSDELLMEASEGQVILYNIRTQQLKEVPVKGH-PA 360

Query: 361 RSQATVFIQTLSSV 363
           R Q TVF+++L SV
Sbjct: 361 RFQGTVFVKSLMSV 370

BLAST of Lag0031534 vs. NCBI nr
Match: XP_022935692.1 (F-box protein CPR30-like [Cucurbita moschata])

HSP 1 Score: 348.6 bits (893), Expect = 6.7e-92
Identity = 203/374 (54.28%), Postives = 251/374 (67.11%), Query Frame = 0

Query: 1   MANLEDLPEEVMMMILLRVPPESLARFKLVAKSWYALINDPKFL-----DSLPNPNVLLK 60
           MA L  LPEEVM+ IL R+PPESL RFK V KSWYALIN+PKF      +SL   +VLLK
Sbjct: 1   MAALGHLPEEVMIEILSRLPPESLLRFKTVRKSWYALINNPKFATKHFSNSLKQKHVLLK 60

Query: 61  QLVKNKETGKKAIRFSFLKYRFVVDDFV-VEDVHIPFPGKEFHYHTLVHGHSHGLVCLSG 120
           +LV  K +GKK   FSFLK+   +D  V V DV +PF  ++F Y   + GHSHGL+CL+ 
Sbjct: 61  RLV-TKNSGKKENIFSFLKFPLPLDGSVPVYDVDLPF-DEDFRYFE-IRGHSHGLLCLTD 120

Query: 121 GHDGICLYKPATREFRILPPSIIHINRPYNVND-VNSWTNAVGFGYDPKSKDFKVVRVVD 180
               I L  PATREFR LPPSI+ +  P    D  +S TNAVGFGYD KS+DFKVVRVV+
Sbjct: 121 LRRDIFLCNPATREFRKLPPSILLLTEPPTEPDGYDSSTNAVGFGYDSKSRDFKVVRVVN 180

Query: 181 FT-GP-FSKFLRAEVLDLEKNEWREIETPIPGCRDYQPSFEMYYQGFYYWWALDRIESGF 240
           F  GP +    R E+ DL K+ WREIETPI G   + PSFEMY++G YYWWA        
Sbjct: 181 FVEGPGYFHPPRVEIYDLSKDRWREIETPICGHVFWAPSFEMYHEGTYYWWADTNEGVEI 240

Query: 241 IMSFDMSEEVFRMIPAPESFQ--MYHYKSMGVLNGSIVLFYYPWGADKKSFEVWKMVKDD 300
           I SFDMSEEVF  I  PESF+     Y+S+GVLNGSIVLF YP   D++ F++W M KD+
Sbjct: 241 IQSFDMSEEVFGQISMPESFEEKREKYRSLGVLNGSIVLFRYPSRGDEREFDIWGMEKDE 300

Query: 301 -ELVSWSKMFTFSPDSGVEFPVAFVGSHQLLMETNEGQLILYNVNTQRFKHLPLHGGLPS 360
              VSWSK+    P  G+E P+ FV   +LLME NEGQ++LYN  TQ+ K LP++G  P+
Sbjct: 301 FGGVSWSKLLRIGPLRGIEKPLVFVSFDELLMEANEGQIVLYNARTQQLKELPINGH-PA 360

Query: 361 RSQATVFIQTLSSV 363
           R QAT+F+++L SV
Sbjct: 361 RFQATLFVKSLVSV 370

BLAST of Lag0031534 vs. NCBI nr
Match: XP_022159408.1 (F-box/kelch-repeat protein At3g23880-like [Momordica charantia])

HSP 1 Score: 340.5 bits (872), Expect = 1.8e-89
Identity = 203/376 (53.99%), Postives = 247/376 (65.69%), Query Frame = 0

Query: 2   ANLEDLPEEVMMMILLRVPPESLARFKLVAKSWYALINDPKFLDS------LPNPNVLLK 61
           A+L +LPEEVM+ IL R+PPESL RFK V KSWYALIN+P F           +  VLLK
Sbjct: 3   ASLGNLPEEVMIEILSRLPPESLVRFKCVRKSWYALINNPNFATKHFANFPQSSTTVLLK 62

Query: 62  QLVKNKETGKKAIRFSFLKYRFVVDDF---VVEDVHIPFPGKEFHYHTLVHGHSHGLVCL 121
           +LV  +E+G K   F+FLK  F + D     V D  +PF  + F Y     GHSHGLVCL
Sbjct: 63  RLV-TEESGNKQNMFTFLK--FPLHDHQSASVLDFDLPF-DENFQYFEF-RGHSHGLVCL 122

Query: 122 SGGHDGICLYKPATREFRILPPSIIHINRPYNVND-VNSWTNAVGFGYDPKSKDFKVVRV 181
           S     I L  PATREFR LPPSI+ +  P    D  +S TNAVGFGYDP+S+DFKVVRV
Sbjct: 123 SDLRSDIILCNPATREFRKLPPSILLLTEPPEEPDGYDSSTNAVGFGYDPRSRDFKVVRV 182

Query: 182 VDFT-GP-FSKFLRAEVLDLEKNEWREIETPIPGCRDYQPSFEMYYQGFYYWWALDRIES 241
           V+F  GP F    R EV DL K+ WREI+TP+ G   + PSF+MY++G YYWWA     S
Sbjct: 183 VNFVEGPGFFHPPRVEVYDLGKDRWREIKTPVCGHVFWAPSFDMYHEGMYYWWADAEGGS 242

Query: 242 GFIMSFDMSEEVFRMIPAPESFQMYH--YKSMGVLNGSIVLFYYPWGADKKSFEVWKMVK 301
             I SFDMSEEVFR IP PESF+     Y+SMGV  GSIVLF+Y  GAD+K+F++W M K
Sbjct: 243 EIIQSFDMSEEVFRPIPVPESFEELREKYRSMGVWKGSIVLFHYLPGADEKTFDIWAMEK 302

Query: 302 DDE-LVSWSKMFTFSPDSGVEFPVAFVGSHQLLMETNEGQLILYNVNTQRFKHLPLHGGL 361
           D+   VSWSK  T  P SG+E P+ FV S +LLME  +GQ+ILYNVNT + K LP+ G  
Sbjct: 303 DNSGGVSWSKALTIGPVSGIEKPLVFVSSDELLMEAKDGQVILYNVNTGQVKELPVKGH- 362

Query: 362 PSRSQATVFIQTLSSV 363
           P R  ATV +++L SV
Sbjct: 363 PGRFHATVLLKSLVSV 372

BLAST of Lag0031534 vs. ExPASy Swiss-Prot
Match: Q9SU30 (F-box protein CPR1 OS=Arabidopsis thaliana OX=3702 GN=CPR1 PE=1 SV=2)

HSP 1 Score: 106.7 bits (265), Expect = 5.8e-22
Identity = 104/386 (26.94%), Postives = 175/386 (45.34%), Query Frame = 0

Query: 7   LPEEVMMMILLRVPPESLARFKLVAKSWYALINDPKFLDSLPNPNVLLKQLVKNKETGKK 66
           +P +++  I LR+P ++L R + ++K  Y LINDP F++S       L ++++  +    
Sbjct: 4   IPMDIVNDIFLRLPAKTLVRCRALSKPCYHLINDPDFIES------HLHRVLQTGDHLMI 63

Query: 67  AIRFSFLKYRFVVDDF-VVEDVHIPFPGKEFHYHTLVHGHSHGLVCLSGGHDGICLYKPA 126
            +R +   Y   +D    V DV  P    +    T V G S+GL+ LS     + ++ P+
Sbjct: 64  LLRGALRLYSVDLDSLDSVSDVEHPM---KRGGPTEVFGSSNGLIGLSNSPTDLAVFNPS 123

Query: 127 TREFRILPPSIIHINRPYNVNDVNSWTNAV--GFGYDPKSKDFKVVRVVDFTGPFSKFL- 186
           TR+   LPPS I      ++ D +S    V  G GYD  S D+KVVR+V F       L 
Sbjct: 124 TRQIHRLPPSSI------DLPDGSSTRGYVFYGLGYDSVSDDYKVVRMVQFKIDSEDELG 183

Query: 187 -----RAEVLDLEKNEWREIETPIPGCRDYQPSFEMYYQGFY-----------YWWALDR 246
                  +V  L+KN W+ IE+        Q  F  YY   Y             W L R
Sbjct: 184 CSFPYEVKVFSLKKNSWKRIESV---ASSIQLLFYFYYHLLYRRGYGVLAGNSLHWVLPR 243

Query: 247 ----IESGFIMSFDMSEEVFRMIPAPESF---QMYHYKSMGVLNGSIVLFYYPWGADKKS 306
               I    I+ FD++ E F ++  PE+     +     +GVL+G + L       D+  
Sbjct: 244 RPGLIAFNLIVRFDLALEEFEIVRFPEAVANGNVDIQMDIGVLDGCLCLM---CNYDQSY 303

Query: 307 FEVWKMVKDDELVSWSKMFTFSPDSGVEF-----PVAF-VGSHQLLMETNEGQLILYNVN 360
            +VW M + +   SW+K+FT      V+      P+ +     ++L+E N  +L+ +++ 
Sbjct: 304 VDVWMMKEYNVRDSWTKVFTVQKPKSVKSFSYMRPLVYSKDKKKVLLELNNTKLVWFDLE 363

BLAST of Lag0031534 vs. ExPASy Swiss-Prot
Match: Q8GXC7 (F-box/kelch-repeat protein At3g06240 OS=Arabidopsis thaliana OX=3702 GN=At3g06240 PE=2 SV=1)

HSP 1 Score: 98.2 bits (243), Expect = 2.1e-19
Identity = 101/397 (25.44%), Postives = 179/397 (45.09%), Query Frame = 0

Query: 7   LPEEVMMMILLRVPPESLARFKLVAKSWYALINDPKF----LD-SLPNPNV--LLKQLVK 66
           LP E++  ILLR+P +S+ RF+ V+K +  L +DP F    LD  L N +V  L ++L+ 
Sbjct: 36  LPPEIITEILLRLPAKSIGRFRCVSKLFCTLSSDPGFAKIHLDLILRNESVRSLHRKLIV 95

Query: 67  NKET--------------GKKAIRFSF-LK---------YRFVVDDFVVED--VHIPFPG 126
           +                    A+  ++ LK          R  V D + +D  V +    
Sbjct: 96  SSHNLYSLDFNSIGDGIRDLAAVEHNYPLKDDPSIFSEMIRNYVGDHLYDDRRVMLKLNA 155

Query: 127 KEFHYHTL-VHGHSHGLVCLSGGHDGICLYKPATREFRILPPSIIHINRPYNVNDVNSWT 186
           K +  + + + G S+GLVC+S G   + LY P T + + LP +     RP +V       
Sbjct: 156 KSYRRNWVEIVGSSNGLVCISPGEGAVFLYNPTTGDSKRLPENF----RPKSVEYERDNF 215

Query: 187 NAVGFGYDPKSKDFKVVRVVDFTGPFSKFLRAEVLDLEKNEWREIETPIPGCRDYQPSFE 246
              GFG+D  + D+K+V++V         L A V  L+ + WR I        D   +  
Sbjct: 216 QTYGFGFDGLTDDYKLVKLV---ATSEDILDASVYSLKADSWRRICNLNYEHNDGSYTSG 275

Query: 247 MYYQGFYYW-WALDRIESGFIMSFDMSEEVFRMIPAPESFQMYHYK----SMGVLNGSIV 306
           +++ G  +W +   R     +++FD+  E FR +P P+  +   ++     +G LNG + 
Sbjct: 276 VHFNGAIHWVFTESRHNQRVVVAFDIQTEEFREMPVPDEAEDCSHRFSNFVVGSLNGRLC 335

Query: 307 LFYYPWGADKKSFEVWKMVKDDELVSWSKMFTFSPDSGVEFPVAFVGSHQLLMETNEGQL 364
           +    +       ++W M +  E  SWS++        ++   +     ++L+E  +G L
Sbjct: 336 VVNSCYDVHD---DIWVMSEYGEAKSWSRIRINLLYRSMKPLCSTKNDEEVLLEL-DGDL 395

BLAST of Lag0031534 vs. ExPASy Swiss-Prot
Match: Q9LIR8 (F-box/kelch-repeat protein At3g23880 OS=Arabidopsis thaliana OX=3702 GN=At3g23880 PE=2 SV=1)

HSP 1 Score: 97.4 bits (241), Expect = 3.5e-19
Identity = 99/376 (26.33%), Postives = 163/376 (43.35%), Query Frame = 0

Query: 1   MANLEDLPEEVMMMILLRVPPESLARFKLVAKSWYALINDPKFLDSLPNPNVL--LKQLV 60
           M +  +LP E+M  ILLR+P +SL RFK V  SW +LI++  F  +L +  +L   K   
Sbjct: 8   MFSPHNLPLEMMEEILLRLPVKSLTRFKCVCSSWRSLISETLF--ALKHALILETSKATT 67

Query: 61  KNKE------TGKKAIRFSFLKYRFVVDDFVVEDVHIPFPGKEFHYHTLVHGHSHGLVCL 120
             K       T +  ++   +   +      V +      G++++    V G  HGLVC 
Sbjct: 68  STKSPYGVITTSRYHLKSCCIHSLYNASTVYVSEHDGELLGRDYYQ---VVGTCHGLVCF 127

Query: 121 SGGHD-GICLYKPATREFRILPPSIIHINRPYNVNDVNSWTNAVGFGYDPKSKDFKVVRV 180
              +D  + L+ P  +  + L  S +  +        +      GFGYD    D+KVV +
Sbjct: 128 HVDYDKSLYLWNPTIKLQQRLSSSDLETSD-------DECVVTYGFGYDESEDDYKVVAL 187

Query: 181 VDFTGPFSKFLRAEVLDLEKNEWREIETPIPG---CRDYQPSFEMYYQGFYYWWALDRIE 240
           +         +  ++    +  WR   T  P      D   S  +Y  G   W A     
Sbjct: 188 LQQRHQVK--IETKIYSTRQKLWRS-NTSFPSGVVVADKSRS-GIYINGTLNWAATSSSS 247

Query: 241 SGFIMSFDMSEEVFRMIPAPESFQMYHY-KSMGVLNGSIVLFYYPWGADKKSFEVWKMVK 300
           S  I+S+DMS + F+ +P P       +  ++G L G + +  Y  GA+    +VW M +
Sbjct: 248 SWTIISYDMSRDEFKELPGPVCCGRGCFTMTLGDLRGCLSMVCYCKGANA---DVWVMKE 307

Query: 301 DDELVSWSKMFTFSPDSGVEFPVAFVGSHQLLMETNEGQLILYNVNTQRFKHLPLHGGLP 360
             E+ SWSK+ +    +    P+       +L+E   G L LYN +  RF H P+   + 
Sbjct: 308 FGEVYSWSKLLSIPGLTDFVRPLWISDGLVVLLEFRSG-LALYNCSNGRF-HYPVSNSIS 362

Query: 361 SRSQATVFIQTLSSVN 364
               A V+++T+ S N
Sbjct: 368 GCRDAKVYLKTMVSPN 362

BLAST of Lag0031534 vs. ExPASy Swiss-Prot
Match: Q9LU24 (Putative F-box protein At3g16210 OS=Arabidopsis thaliana OX=3702 GN=At3g16210 PE=4 SV=1)

HSP 1 Score: 85.9 bits (211), Expect = 1.1e-15
Identity = 81/307 (26.38%), Postives = 130/307 (42.35%), Query Frame = 0

Query: 7   LPEEVMMMILLRVPPESLARFKLVAKSWYALINDPKFLDSLPNPNVLLKQLVKNKETGKK 66
           LPEE+ + IL+R+  + LARF+ V K+W  LINDP F ++  + +               
Sbjct: 5   LPEELAIEILVRLSMKDLARFRCVCKTWRDLINDPGFTETYRDMS--------------P 64

Query: 67  AIRFSFLKYRFVVDD------FVVEDVHIPFPGKEFHYHTLVHGHSHGLVCLSGGHDGIC 126
           A   SF    F + D       +   +  P         T V  H  G +C++  +  + 
Sbjct: 65  AKFVSFYDKNFYMLDVEGKHPVITNKLDFPLDQSMIDESTCVL-HCDGTLCVTLKNHTLM 124

Query: 127 LYKPATREFRILPPSIIHINRPYNVNDVNSWTNAVGFGYDPKSKDFKVVRVVDFTGPFSK 186
           ++ P +++F+I+P   I+ +           +N +GFGYDP   D+KVV  +D     + 
Sbjct: 125 VWNPFSKQFKIVPNPGIYQD-----------SNILGFGYDPVHDDYKVVTFIDRLDVST- 184

Query: 187 FLRAEVLDLEKNEWRE---IETPIPGCRDYQPSFEMYYQGFYYWWALDRIESGFIMSFDM 246
              A V +     W E   I  P    RD + +F      + YW A       FI+ F++
Sbjct: 185 ---AHVFEFRTGSWGESLRISYPDWHYRDRRGTF---LDQYLYWIAYRSSADRFILCFNL 244

Query: 247 SEEVFRMIPAPESFQMYHYKSMGVLNGSIVLFYYPWGADKKSFEVWKMVKDDELVSWSKM 305
           S   +R +P P   Q      +GV +  + +  Y     KK   +  M K     SWSK+
Sbjct: 245 STHEYRKLPLPVYNQGVTSSWLGVTSQKLCITEYE--MCKKEIRISVMEKTG---SWSKI 273

BLAST of Lag0031534 vs. ExPASy Swiss-Prot
Match: Q9FGY4 (F-box protein At5g49610 OS=Arabidopsis thaliana OX=3702 GN=At5g49610 PE=1 SV=1)

HSP 1 Score: 72.4 bits (176), Expect = 1.2e-11
Identity = 92/358 (25.70%), Postives = 154/358 (43.02%), Query Frame = 0

Query: 8   PEEVMMMILLRVPPESLARFKLVAKSWYALINDPKFLDSLPNPNVLLKQLVKNKETGKKA 67
           P+EV++ IL R+P +SL RFK V KSWY L +D K+  SL N   + +QL+  + +   +
Sbjct: 10  PDEVILQILARLPVKSLFRFKSVCKSWYRLPSD-KYFTSLFNQLSVKEQLLVAEVSDSSS 69

Query: 68  IRFSFLKYRFVVDDF-VVEDVHIPFPGKEFHYHTLVHGHSHGLVCLSGGHDGICLY--KP 127
           +          VD+   V ++ + F          +   S+GL+C S   +    Y   P
Sbjct: 70  L--------ICVDNLRGVSELSLDF----VRDRVRIRVSSNGLLCCSSIPEKGVYYVCNP 129

Query: 128 ATREFRILPPSIIHINRPYNVNDVNSWTNAVGFGYDPKSKDFKVVRVVDFTG----PFSK 187
           +TRE+R LP S     RP      +     VG   D     F VV           P   
Sbjct: 130 STREYRKLPKS---RERPVTRFYPDGEATLVGLACDLSKNKFNVVLAGYHRSFGQRPDGS 189

Query: 188 FLRAEVLDLEKNEWREIETPIPGCRDYQPSFE--MYYQGFYYWWALDRIESG--FIMSFD 247
           F+   V D E N+WR+  + +  C     S    ++  G  +W     + SG  +I++ D
Sbjct: 190 FI-CLVFDSESNKWRKFVSVLEECSFTHMSKNQVVFVNGMLHW-----LMSGLCYILALD 249

Query: 248 MSEEVFRMIPAPESF--------QMYHYKSMGVLNGSIVLFYYPWGADKKSFEVWKMVKD 307
           +  +V+R I  P+          ++Y  +S G L  S++     W       ++WKM  +
Sbjct: 250 VEHDVWRKISLPDEIRIGNGGGNRVYLLESDGFL--SVIQLSDVW------MKIWKM-SE 309

Query: 308 DELVSWSKMFTFSPD------SGVEFPVAFVGSHQLLMETNEGQLILYNVNTQRFKHL 341
            E  +WS + + S         G+ FP+   G +  L      Q+++Y   ++ +K +
Sbjct: 310 YETETWSVVDSISLRCIKGLVPGI-FPICQTGEYVFL--ATHKQVLVYQRRSKLWKEM 333

BLAST of Lag0031534 vs. ExPASy TrEMBL
Match: A0A6J1J6Y5 (F-box protein CPR1-like OS=Cucurbita maxima OX=3661 GN=LOC111482312 PE=4 SV=1)

HSP 1 Score: 357.5 bits (916), Expect = 7.0e-95
Identity = 207/375 (55.20%), Postives = 256/375 (68.27%), Query Frame = 0

Query: 1   MANLEDLPEEVMMMILLRVPPESLARFKLVAKSWYALINDPKFL-----DSLPNPNVLLK 60
           MA L  LPEEVM+ IL R+PPESL RFK V KSWYALI++PKF      +SL + +VLLK
Sbjct: 1   MAALGHLPEEVMIEILSRLPPESLLRFKTVRKSWYALIDNPKFATKHFSNSLKHKHVLLK 60

Query: 61  QLVKNKETGKKAIRFSFLKYRFVVDDFV-VEDVHIPFPGKEFHYHTLVHGHSHGLVCLSG 120
           +LV  K +GKK   FSFLK+   +D  V V DV  PF  ++F Y   + GHSHGL+CL+ 
Sbjct: 61  RLV-TKNSGKKENIFSFLKFPLPLDGSVPVFDVDWPF-DEDFRYFE-IRGHSHGLLCLTD 120

Query: 121 GHDGICLYKPATREFRILPPSIIHINRPYNVND-VNSWTNAVGFGYDPKSKDFKVVRVVD 180
               I L  PATREFR LP SI+ +  P    D  +S TNAVGFGYD KSKDFKVVRVV+
Sbjct: 121 LRRDIFLCNPATREFRKLPQSILLLTEPPTEPDGYDSSTNAVGFGYDSKSKDFKVVRVVN 180

Query: 181 FT-GP-FSKFLRAEVLDLEKNEWREIETPIPGCRDYQPSFEMYYQGFYYWWALDRIESGF 240
           F  GP +    RAE+ DL K+ WREIETPI G   + PSFEMY++G YYWWA    +   
Sbjct: 181 FVEGPGYFHPPRAEIYDLSKDRWREIETPICGHVFWAPSFEMYHEGTYYWWADTDDKEEI 240

Query: 241 IMSFDMSEEVFRMIPAPESF--QMYHYKSMGVLNGSIVLFYYPWGADKKSFEVWKMVKDD 300
           I SFDMSEEVFR IP PESF  +   Y+SMG+LNGSIVLF+YP   D++ F++W M KD+
Sbjct: 241 IQSFDMSEEVFRRIPVPESFEGERERYRSMGILNGSIVLFHYPSRGDEREFDIWGMEKDE 300

Query: 301 -ELVSWSKMFTFSPDSGVEFPVAFVGSHQLLMETNEGQLILYNVNTQRFKHLPLHGGLPS 360
              VSWSK+ T  P  G+E P+ FV S +LLME NEGQ++LYN   Q+ K LP+ G  P+
Sbjct: 301 FGGVSWSKLLTIGPLRGIEKPLVFVSSDELLMEANEGQIVLYNARIQQLKELPIKGH-PA 360

Query: 361 RSQATVFIQTLSSVN 364
           R QAT+F+++L SVN
Sbjct: 361 RFQATLFVKSLVSVN 371

BLAST of Lag0031534 vs. ExPASy TrEMBL
Match: A0A6J1F6B1 (F-box protein CPR30-like OS=Cucurbita moschata OX=3662 GN=LOC111442474 PE=4 SV=1)

HSP 1 Score: 348.6 bits (893), Expect = 3.2e-92
Identity = 203/374 (54.28%), Postives = 251/374 (67.11%), Query Frame = 0

Query: 1   MANLEDLPEEVMMMILLRVPPESLARFKLVAKSWYALINDPKFL-----DSLPNPNVLLK 60
           MA L  LPEEVM+ IL R+PPESL RFK V KSWYALIN+PKF      +SL   +VLLK
Sbjct: 1   MAALGHLPEEVMIEILSRLPPESLLRFKTVRKSWYALINNPKFATKHFSNSLKQKHVLLK 60

Query: 61  QLVKNKETGKKAIRFSFLKYRFVVDDFV-VEDVHIPFPGKEFHYHTLVHGHSHGLVCLSG 120
           +LV  K +GKK   FSFLK+   +D  V V DV +PF  ++F Y   + GHSHGL+CL+ 
Sbjct: 61  RLV-TKNSGKKENIFSFLKFPLPLDGSVPVYDVDLPF-DEDFRYFE-IRGHSHGLLCLTD 120

Query: 121 GHDGICLYKPATREFRILPPSIIHINRPYNVND-VNSWTNAVGFGYDPKSKDFKVVRVVD 180
               I L  PATREFR LPPSI+ +  P    D  +S TNAVGFGYD KS+DFKVVRVV+
Sbjct: 121 LRRDIFLCNPATREFRKLPPSILLLTEPPTEPDGYDSSTNAVGFGYDSKSRDFKVVRVVN 180

Query: 181 FT-GP-FSKFLRAEVLDLEKNEWREIETPIPGCRDYQPSFEMYYQGFYYWWALDRIESGF 240
           F  GP +    R E+ DL K+ WREIETPI G   + PSFEMY++G YYWWA        
Sbjct: 181 FVEGPGYFHPPRVEIYDLSKDRWREIETPICGHVFWAPSFEMYHEGTYYWWADTNEGVEI 240

Query: 241 IMSFDMSEEVFRMIPAPESFQ--MYHYKSMGVLNGSIVLFYYPWGADKKSFEVWKMVKDD 300
           I SFDMSEEVF  I  PESF+     Y+S+GVLNGSIVLF YP   D++ F++W M KD+
Sbjct: 241 IQSFDMSEEVFGQISMPESFEEKREKYRSLGVLNGSIVLFRYPSRGDEREFDIWGMEKDE 300

Query: 301 -ELVSWSKMFTFSPDSGVEFPVAFVGSHQLLMETNEGQLILYNVNTQRFKHLPLHGGLPS 360
              VSWSK+    P  G+E P+ FV   +LLME NEGQ++LYN  TQ+ K LP++G  P+
Sbjct: 301 FGGVSWSKLLRIGPLRGIEKPLVFVSFDELLMEANEGQIVLYNARTQQLKELPINGH-PA 360

Query: 361 RSQATVFIQTLSSV 363
           R QAT+F+++L SV
Sbjct: 361 RFQATLFVKSLVSV 370

BLAST of Lag0031534 vs. ExPASy TrEMBL
Match: A0A6J1DYQ4 (F-box/kelch-repeat protein At3g23880-like OS=Momordica charantia OX=3673 GN=LOC111025827 PE=4 SV=1)

HSP 1 Score: 340.5 bits (872), Expect = 8.8e-90
Identity = 203/376 (53.99%), Postives = 247/376 (65.69%), Query Frame = 0

Query: 2   ANLEDLPEEVMMMILLRVPPESLARFKLVAKSWYALINDPKFLDS------LPNPNVLLK 61
           A+L +LPEEVM+ IL R+PPESL RFK V KSWYALIN+P F           +  VLLK
Sbjct: 3   ASLGNLPEEVMIEILSRLPPESLVRFKCVRKSWYALINNPNFATKHFANFPQSSTTVLLK 62

Query: 62  QLVKNKETGKKAIRFSFLKYRFVVDDF---VVEDVHIPFPGKEFHYHTLVHGHSHGLVCL 121
           +LV  +E+G K   F+FLK  F + D     V D  +PF  + F Y     GHSHGLVCL
Sbjct: 63  RLV-TEESGNKQNMFTFLK--FPLHDHQSASVLDFDLPF-DENFQYFEF-RGHSHGLVCL 122

Query: 122 SGGHDGICLYKPATREFRILPPSIIHINRPYNVND-VNSWTNAVGFGYDPKSKDFKVVRV 181
           S     I L  PATREFR LPPSI+ +  P    D  +S TNAVGFGYDP+S+DFKVVRV
Sbjct: 123 SDLRSDIILCNPATREFRKLPPSILLLTEPPEEPDGYDSSTNAVGFGYDPRSRDFKVVRV 182

Query: 182 VDFT-GP-FSKFLRAEVLDLEKNEWREIETPIPGCRDYQPSFEMYYQGFYYWWALDRIES 241
           V+F  GP F    R EV DL K+ WREI+TP+ G   + PSF+MY++G YYWWA     S
Sbjct: 183 VNFVEGPGFFHPPRVEVYDLGKDRWREIKTPVCGHVFWAPSFDMYHEGMYYWWADAEGGS 242

Query: 242 GFIMSFDMSEEVFRMIPAPESFQMYH--YKSMGVLNGSIVLFYYPWGADKKSFEVWKMVK 301
             I SFDMSEEVFR IP PESF+     Y+SMGV  GSIVLF+Y  GAD+K+F++W M K
Sbjct: 243 EIIQSFDMSEEVFRPIPVPESFEELREKYRSMGVWKGSIVLFHYLPGADEKTFDIWAMEK 302

Query: 302 DDE-LVSWSKMFTFSPDSGVEFPVAFVGSHQLLMETNEGQLILYNVNTQRFKHLPLHGGL 361
           D+   VSWSK  T  P SG+E P+ FV S +LLME  +GQ+ILYNVNT + K LP+ G  
Sbjct: 303 DNSGGVSWSKALTIGPVSGIEKPLVFVSSDELLMEAKDGQVILYNVNTGQVKELPVKGH- 362

Query: 362 PSRSQATVFIQTLSSV 363
           P R  ATV +++L SV
Sbjct: 363 PGRFHATVLLKSLVSV 372

BLAST of Lag0031534 vs. ExPASy TrEMBL
Match: A0A5A7UHP3 (F-box protein CPR30-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold135G001510 PE=4 SV=1)

HSP 1 Score: 330.5 bits (846), Expect = 9.1e-87
Identity = 184/374 (49.20%), Postives = 247/374 (66.04%), Query Frame = 0

Query: 1   MANLEDLPEEVMMMILLRVPPESLARFKLVAKSWYALINDPK-----FLDSLPNPNVLLK 60
           MA+L +LP+ V++ IL R+PPESL RFK V KSWYAL NDPK     F  SL + ++LLK
Sbjct: 1   MASLGNLPDGVIIEILSRLPPESLLRFKCVRKSWYALFNDPKFKAKHFSTSLQHKHLLLK 60

Query: 61  QLVKNKETGKKAIRFSFLKYRFVV-DDFVVEDVHIPFPGKEFHYHTLVHGHSHGLVCLSG 120
           +LV  K++GKK   FS LK    +     + D+ +PF  ++F +   +HGHSHGL+CL+ 
Sbjct: 61  RLV-TKDSGKKENIFSILKLPLSIHSSLSISDIDLPF-HEDFRFFE-IHGHSHGLLCLTD 120

Query: 121 GHDGICLYKPATREFRILPPSIIHINRP-YNVNDVNSWTNAVGFGYDPKSKDFKVVRVVD 180
               I L  P+TREF  LPPSI+ +  P    +D +S TNAVGFGYD KS+DFKVVRVVD
Sbjct: 121 LRKDIFLCNPSTREFHQLPPSILLLPEPSAEPDDYDSSTNAVGFGYDSKSRDFKVVRVVD 180

Query: 181 FTGPFSKFL--RAEVLDLEKNEWREIETPIPGCRDYQPSFEMYYQGFYYWWALDRIESGF 240
           F      F   + EV DL K  WREI+TP+ G   + P FE++++G YYWWA     +  
Sbjct: 181 FVEGPGYFYPPKVEVYDLSKERWREIQTPVSGHVFWAPCFEVFHEGTYYWWADADGNTEI 240

Query: 241 IMSFDMSEEVFRMIPAPESFQMY--HYKSMGVLNGSIVLFYYPWGADKKSFEVWKMVKDD 300
           I++FDMSEEVF  IP PESFQ     Y+S+GVL+G IVLF+YP   D++ F++W+M KD+
Sbjct: 241 ILTFDMSEEVFGQIPVPESFQGTGDWYRSLGVLDGRIVLFHYPSRGDERRFDMWEMGKDE 300

Query: 301 E-LVSWSKMFTFSPDSGVEFPVAFVGSHQLLMETNEGQLILYNVNTQRFKHLPLHGGLPS 360
              VSWSK+ T  P SG+E P+ F+ S +LLME N GQLI+YN+ ++  K +P+ G  P 
Sbjct: 301 SGGVSWSKVLTIGPVSGIEKPLLFISSEELLMEGNGGQLIVYNIRSEEVKEVPIKGS-PG 360

Query: 361 RSQATVFIQTLSSV 363
           R Q T F+++L SV
Sbjct: 361 RFQGTAFVKSLLSV 370

BLAST of Lag0031534 vs. ExPASy TrEMBL
Match: A0A5D3DPN6 (F-box protein CPR30-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G007960 PE=4 SV=1)

HSP 1 Score: 330.5 bits (846), Expect = 9.1e-87
Identity = 184/374 (49.20%), Postives = 247/374 (66.04%), Query Frame = 0

Query: 1   MANLEDLPEEVMMMILLRVPPESLARFKLVAKSWYALINDPK-----FLDSLPNPNVLLK 60
           MA+L +LP+ V++ IL R+PPESL RFK V KSWYAL NDPK     F  SL + ++LLK
Sbjct: 1   MASLGNLPDGVIIEILSRLPPESLLRFKCVRKSWYALFNDPKFKAKHFSTSLQHKHLLLK 60

Query: 61  QLVKNKETGKKAIRFSFLKYRFVV-DDFVVEDVHIPFPGKEFHYHTLVHGHSHGLVCLSG 120
           +LV  K++GKK   FS LK    +     + D+ +PF  ++F +   +HGHSHGL+CL+ 
Sbjct: 61  RLV-TKDSGKKENIFSILKLPLSIHSSLSISDIDLPF-HEDFRFFE-IHGHSHGLLCLTD 120

Query: 121 GHDGICLYKPATREFRILPPSIIHINRP-YNVNDVNSWTNAVGFGYDPKSKDFKVVRVVD 180
               I L  P+TREF  LPPSI+ +  P    +D +S TNAVGFGYD KS+DFKVVRVVD
Sbjct: 121 LRKDIFLCNPSTREFHQLPPSILLLPEPSAEPDDYDSSTNAVGFGYDSKSRDFKVVRVVD 180

Query: 181 FTGPFSKFL--RAEVLDLEKNEWREIETPIPGCRDYQPSFEMYYQGFYYWWALDRIESGF 240
           F      F   + EV DL K  WREI+TP+ G   + P FE++++G YYWWA     +  
Sbjct: 181 FVEGPGYFYPPKVEVYDLSKERWREIQTPVSGHVFWAPCFEVFHEGTYYWWADADGNTEI 240

Query: 241 IMSFDMSEEVFRMIPAPESFQMY--HYKSMGVLNGSIVLFYYPWGADKKSFEVWKMVKDD 300
           I++FDMSEEVF  IP PESFQ     Y+S+GVL+G IVLF+YP   D++ F++W+M KD+
Sbjct: 241 ILTFDMSEEVFGQIPVPESFQGTGDWYRSLGVLDGCIVLFHYPSRGDERRFDMWEMGKDE 300

Query: 301 E-LVSWSKMFTFSPDSGVEFPVAFVGSHQLLMETNEGQLILYNVNTQRFKHLPLHGGLPS 360
              VSWSK+ T  P SG+E P+ F+ S +LLME N GQLI+YN+ ++  K +P+ G  P 
Sbjct: 301 SGGVSWSKVLTIGPVSGIEKPLLFISSEELLMEGNGGQLIVYNIRSEEVKEVPIKGS-PG 360

Query: 361 RSQATVFIQTLSSV 363
           R Q T F+++L SV
Sbjct: 361 RFQGTAFVKSLLSV 370

BLAST of Lag0031534 vs. TAIR 10
Match: AT4G12560.1 (F-box and associated interaction domains-containing protein )

HSP 1 Score: 106.7 bits (265), Expect = 4.1e-23
Identity = 104/386 (26.94%), Postives = 175/386 (45.34%), Query Frame = 0

Query: 7   LPEEVMMMILLRVPPESLARFKLVAKSWYALINDPKFLDSLPNPNVLLKQLVKNKETGKK 66
           +P +++  I LR+P ++L R + ++K  Y LINDP F++S       L ++++  +    
Sbjct: 4   IPMDIVNDIFLRLPAKTLVRCRALSKPCYHLINDPDFIES------HLHRVLQTGDHLMI 63

Query: 67  AIRFSFLKYRFVVDDF-VVEDVHIPFPGKEFHYHTLVHGHSHGLVCLSGGHDGICLYKPA 126
            +R +   Y   +D    V DV  P    +    T V G S+GL+ LS     + ++ P+
Sbjct: 64  LLRGALRLYSVDLDSLDSVSDVEHPM---KRGGPTEVFGSSNGLIGLSNSPTDLAVFNPS 123

Query: 127 TREFRILPPSIIHINRPYNVNDVNSWTNAV--GFGYDPKSKDFKVVRVVDFTGPFSKFL- 186
           TR+   LPPS I      ++ D +S    V  G GYD  S D+KVVR+V F       L 
Sbjct: 124 TRQIHRLPPSSI------DLPDGSSTRGYVFYGLGYDSVSDDYKVVRMVQFKIDSEDELG 183

Query: 187 -----RAEVLDLEKNEWREIETPIPGCRDYQPSFEMYYQGFY-----------YWWALDR 246
                  +V  L+KN W+ IE+        Q  F  YY   Y             W L R
Sbjct: 184 CSFPYEVKVFSLKKNSWKRIESV---ASSIQLLFYFYYHLLYRRGYGVLAGNSLHWVLPR 243

Query: 247 ----IESGFIMSFDMSEEVFRMIPAPESF---QMYHYKSMGVLNGSIVLFYYPWGADKKS 306
               I    I+ FD++ E F ++  PE+     +     +GVL+G + L       D+  
Sbjct: 244 RPGLIAFNLIVRFDLALEEFEIVRFPEAVANGNVDIQMDIGVLDGCLCLM---CNYDQSY 303

Query: 307 FEVWKMVKDDELVSWSKMFTFSPDSGVEF-----PVAF-VGSHQLLMETNEGQLILYNVN 360
            +VW M + +   SW+K+FT      V+      P+ +     ++L+E N  +L+ +++ 
Sbjct: 304 VDVWMMKEYNVRDSWTKVFTVQKPKSVKSFSYMRPLVYSKDKKKVLLELNNTKLVWFDLE 363

BLAST of Lag0031534 vs. TAIR 10
Match: AT4G12560.2 (F-box and associated interaction domains-containing protein )

HSP 1 Score: 106.7 bits (265), Expect = 4.1e-23
Identity = 104/386 (26.94%), Postives = 175/386 (45.34%), Query Frame = 0

Query: 7   LPEEVMMMILLRVPPESLARFKLVAKSWYALINDPKFLDSLPNPNVLLKQLVKNKETGKK 66
           +P +++  I LR+P ++L R + ++K  Y LINDP F++S       L ++++  +    
Sbjct: 4   IPMDIVNDIFLRLPAKTLVRCRALSKPCYHLINDPDFIES------HLHRVLQTGDHLMI 63

Query: 67  AIRFSFLKYRFVVDDF-VVEDVHIPFPGKEFHYHTLVHGHSHGLVCLSGGHDGICLYKPA 126
            +R +   Y   +D    V DV  P    +    T V G S+GL+ LS     + ++ P+
Sbjct: 64  LLRGALRLYSVDLDSLDSVSDVEHPM---KRGGPTEVFGSSNGLIGLSNSPTDLAVFNPS 123

Query: 127 TREFRILPPSIIHINRPYNVNDVNSWTNAV--GFGYDPKSKDFKVVRVVDFTGPFSKFL- 186
           TR+   LPPS I      ++ D +S    V  G GYD  S D+KVVR+V F       L 
Sbjct: 124 TRQIHRLPPSSI------DLPDGSSTRGYVFYGLGYDSVSDDYKVVRMVQFKIDSEDELG 183

Query: 187 -----RAEVLDLEKNEWREIETPIPGCRDYQPSFEMYYQGFY-----------YWWALDR 246
                  +V  L+KN W+ IE+        Q  F  YY   Y             W L R
Sbjct: 184 CSFPYEVKVFSLKKNSWKRIESV---ASSIQLLFYFYYHLLYRRGYGVLAGNSLHWVLPR 243

Query: 247 ----IESGFIMSFDMSEEVFRMIPAPESF---QMYHYKSMGVLNGSIVLFYYPWGADKKS 306
               I    I+ FD++ E F ++  PE+     +     +GVL+G + L       D+  
Sbjct: 244 RPGLIAFNLIVRFDLALEEFEIVRFPEAVANGNVDIQMDIGVLDGCLCLM---CNYDQSY 303

Query: 307 FEVWKMVKDDELVSWSKMFTFSPDSGVEF-----PVAF-VGSHQLLMETNEGQLILYNVN 360
            +VW M + +   SW+K+FT      V+      P+ +     ++L+E N  +L+ +++ 
Sbjct: 304 VDVWMMKEYNVRDSWTKVFTVQKPKSVKSFSYMRPLVYSKDKKKVLLELNNTKLVWFDLE 363

BLAST of Lag0031534 vs. TAIR 10
Match: AT3G06240.1 (F-box family protein )

HSP 1 Score: 98.2 bits (243), Expect = 1.5e-20
Identity = 101/397 (25.44%), Postives = 179/397 (45.09%), Query Frame = 0

Query: 7   LPEEVMMMILLRVPPESLARFKLVAKSWYALINDPKF----LD-SLPNPNV--LLKQLVK 66
           LP E++  ILLR+P +S+ RF+ V+K +  L +DP F    LD  L N +V  L ++L+ 
Sbjct: 36  LPPEIITEILLRLPAKSIGRFRCVSKLFCTLSSDPGFAKIHLDLILRNESVRSLHRKLIV 95

Query: 67  NKET--------------GKKAIRFSF-LK---------YRFVVDDFVVED--VHIPFPG 126
           +                    A+  ++ LK          R  V D + +D  V +    
Sbjct: 96  SSHNLYSLDFNSIGDGIRDLAAVEHNYPLKDDPSIFSEMIRNYVGDHLYDDRRVMLKLNA 155

Query: 127 KEFHYHTL-VHGHSHGLVCLSGGHDGICLYKPATREFRILPPSIIHINRPYNVNDVNSWT 186
           K +  + + + G S+GLVC+S G   + LY P T + + LP +     RP +V       
Sbjct: 156 KSYRRNWVEIVGSSNGLVCISPGEGAVFLYNPTTGDSKRLPENF----RPKSVEYERDNF 215

Query: 187 NAVGFGYDPKSKDFKVVRVVDFTGPFSKFLRAEVLDLEKNEWREIETPIPGCRDYQPSFE 246
              GFG+D  + D+K+V++V         L A V  L+ + WR I        D   +  
Sbjct: 216 QTYGFGFDGLTDDYKLVKLV---ATSEDILDASVYSLKADSWRRICNLNYEHNDGSYTSG 275

Query: 247 MYYQGFYYW-WALDRIESGFIMSFDMSEEVFRMIPAPESFQMYHYK----SMGVLNGSIV 306
           +++ G  +W +   R     +++FD+  E FR +P P+  +   ++     +G LNG + 
Sbjct: 276 VHFNGAIHWVFTESRHNQRVVVAFDIQTEEFREMPVPDEAEDCSHRFSNFVVGSLNGRLC 335

Query: 307 LFYYPWGADKKSFEVWKMVKDDELVSWSKMFTFSPDSGVEFPVAFVGSHQLLMETNEGQL 364
           +    +       ++W M +  E  SWS++        ++   +     ++L+E  +G L
Sbjct: 336 VVNSCYDVHD---DIWVMSEYGEAKSWSRIRINLLYRSMKPLCSTKNDEEVLLEL-DGDL 395

BLAST of Lag0031534 vs. TAIR 10
Match: AT3G23880.1 (F-box and associated interaction domains-containing protein )

HSP 1 Score: 97.4 bits (241), Expect = 2.5e-20
Identity = 99/376 (26.33%), Postives = 163/376 (43.35%), Query Frame = 0

Query: 1   MANLEDLPEEVMMMILLRVPPESLARFKLVAKSWYALINDPKFLDSLPNPNVL--LKQLV 60
           M +  +LP E+M  ILLR+P +SL RFK V  SW +LI++  F  +L +  +L   K   
Sbjct: 8   MFSPHNLPLEMMEEILLRLPVKSLTRFKCVCSSWRSLISETLF--ALKHALILETSKATT 67

Query: 61  KNKE------TGKKAIRFSFLKYRFVVDDFVVEDVHIPFPGKEFHYHTLVHGHSHGLVCL 120
             K       T +  ++   +   +      V +      G++++    V G  HGLVC 
Sbjct: 68  STKSPYGVITTSRYHLKSCCIHSLYNASTVYVSEHDGELLGRDYYQ---VVGTCHGLVCF 127

Query: 121 SGGHD-GICLYKPATREFRILPPSIIHINRPYNVNDVNSWTNAVGFGYDPKSKDFKVVRV 180
              +D  + L+ P  +  + L  S +  +        +      GFGYD    D+KVV +
Sbjct: 128 HVDYDKSLYLWNPTIKLQQRLSSSDLETSD-------DECVVTYGFGYDESEDDYKVVAL 187

Query: 181 VDFTGPFSKFLRAEVLDLEKNEWREIETPIPG---CRDYQPSFEMYYQGFYYWWALDRIE 240
           +         +  ++    +  WR   T  P      D   S  +Y  G   W A     
Sbjct: 188 LQQRHQVK--IETKIYSTRQKLWRS-NTSFPSGVVVADKSRS-GIYINGTLNWAATSSSS 247

Query: 241 SGFIMSFDMSEEVFRMIPAPESFQMYHY-KSMGVLNGSIVLFYYPWGADKKSFEVWKMVK 300
           S  I+S+DMS + F+ +P P       +  ++G L G + +  Y  GA+    +VW M +
Sbjct: 248 SWTIISYDMSRDEFKELPGPVCCGRGCFTMTLGDLRGCLSMVCYCKGANA---DVWVMKE 307

Query: 301 DDELVSWSKMFTFSPDSGVEFPVAFVGSHQLLMETNEGQLILYNVNTQRFKHLPLHGGLP 360
             E+ SWSK+ +    +    P+       +L+E   G L LYN +  RF H P+   + 
Sbjct: 308 FGEVYSWSKLLSIPGLTDFVRPLWISDGLVVLLEFRSG-LALYNCSNGRF-HYPVSNSIS 362

Query: 361 SRSQATVFIQTLSSVN 364
               A V+++T+ S N
Sbjct: 368 GCRDAKVYLKTMVSPN 362

BLAST of Lag0031534 vs. TAIR 10
Match: AT3G16210.1 (F-box family protein )

HSP 1 Score: 85.9 bits (211), Expect = 7.5e-17
Identity = 81/307 (26.38%), Postives = 130/307 (42.35%), Query Frame = 0

Query: 7   LPEEVMMMILLRVPPESLARFKLVAKSWYALINDPKFLDSLPNPNVLLKQLVKNKETGKK 66
           LPEE+ + IL+R+  + LARF+ V K+W  LINDP F ++  + +               
Sbjct: 5   LPEELAIEILVRLSMKDLARFRCVCKTWRDLINDPGFTETYRDMS--------------P 64

Query: 67  AIRFSFLKYRFVVDD------FVVEDVHIPFPGKEFHYHTLVHGHSHGLVCLSGGHDGIC 126
           A   SF    F + D       +   +  P         T V  H  G +C++  +  + 
Sbjct: 65  AKFVSFYDKNFYMLDVEGKHPVITNKLDFPLDQSMIDESTCVL-HCDGTLCVTLKNHTLM 124

Query: 127 LYKPATREFRILPPSIIHINRPYNVNDVNSWTNAVGFGYDPKSKDFKVVRVVDFTGPFSK 186
           ++ P +++F+I+P   I+ +           +N +GFGYDP   D+KVV  +D     + 
Sbjct: 125 VWNPFSKQFKIVPNPGIYQD-----------SNILGFGYDPVHDDYKVVTFIDRLDVST- 184

Query: 187 FLRAEVLDLEKNEWRE---IETPIPGCRDYQPSFEMYYQGFYYWWALDRIESGFIMSFDM 246
              A V +     W E   I  P    RD + +F      + YW A       FI+ F++
Sbjct: 185 ---AHVFEFRTGSWGESLRISYPDWHYRDRRGTF---LDQYLYWIAYRSSADRFILCFNL 244

Query: 247 SEEVFRMIPAPESFQMYHYKSMGVLNGSIVLFYYPWGADKKSFEVWKMVKDDELVSWSKM 305
           S   +R +P P   Q      +GV +  + +  Y     KK   +  M K     SWSK+
Sbjct: 245 STHEYRKLPLPVYNQGVTSSWLGVTSQKLCITEYE--MCKKEIRISVMEKTG---SWSKI 273

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_023526117.18.4e-9553.93F-box protein CPR1-like [Cucurbita pepo subsp. pepo][more]
XP_022983805.11.4e-9455.20F-box protein CPR1-like [Cucurbita maxima][more]
XP_038906036.19.3e-9454.01F-box protein CPR1-like [Benincasa hispida][more]
XP_022935692.16.7e-9254.28F-box protein CPR30-like [Cucurbita moschata][more]
XP_022159408.11.8e-8953.99F-box/kelch-repeat protein At3g23880-like [Momordica charantia][more]
Match NameE-valueIdentityDescription
Q9SU305.8e-2226.94F-box protein CPR1 OS=Arabidopsis thaliana OX=3702 GN=CPR1 PE=1 SV=2[more]
Q8GXC72.1e-1925.44F-box/kelch-repeat protein At3g06240 OS=Arabidopsis thaliana OX=3702 GN=At3g0624... [more]
Q9LIR83.5e-1926.33F-box/kelch-repeat protein At3g23880 OS=Arabidopsis thaliana OX=3702 GN=At3g2388... [more]
Q9LU241.1e-1526.38Putative F-box protein At3g16210 OS=Arabidopsis thaliana OX=3702 GN=At3g16210 PE... [more]
Q9FGY41.2e-1125.70F-box protein At5g49610 OS=Arabidopsis thaliana OX=3702 GN=At5g49610 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1J6Y57.0e-9555.20F-box protein CPR1-like OS=Cucurbita maxima OX=3661 GN=LOC111482312 PE=4 SV=1[more]
A0A6J1F6B13.2e-9254.28F-box protein CPR30-like OS=Cucurbita moschata OX=3662 GN=LOC111442474 PE=4 SV=1[more]
A0A6J1DYQ48.8e-9053.99F-box/kelch-repeat protein At3g23880-like OS=Momordica charantia OX=3673 GN=LOC1... [more]
A0A5A7UHP39.1e-8749.20F-box protein CPR30-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffol... [more]
A0A5D3DPN69.1e-8749.20F-box protein CPR30-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffol... [more]
Match NameE-valueIdentityDescription
AT4G12560.14.1e-2326.94F-box and associated interaction domains-containing protein [more]
AT4G12560.24.1e-2326.94F-box and associated interaction domains-containing protein [more]
AT3G06240.11.5e-2025.44F-box family protein [more]
AT3G23880.12.5e-2026.33F-box and associated interaction domains-containing protein [more]
AT3G16210.17.5e-1726.38F-box family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001810F-box domainSMARTSM00256fbox_2coord: 7..47
e-value: 1.3E-5
score: 34.7
IPR001810F-box domainPFAMPF00646F-boxcoord: 4..43
e-value: 3.4E-8
score: 33.2
IPR001810F-box domainPROSITEPS50181FBOXcoord: 1..47
score: 12.358865
IPR006527F-box associated domain, type 1PFAMPF07734FBA_1coord: 216..322
e-value: 7.7E-9
score: 35.7
NoneNo IPR availableGENE3D1.20.1280.50coord: 1..50
e-value: 6.0E-8
score: 34.3
NoneNo IPR availablePANTHERPTHR47993OS09G0372900 PROTEIN-RELATEDcoord: 4..363
NoneNo IPR availablePANTHERPTHR47993:SF44F-BOX PROTEIN CPR30-LIKE ISOFORM X1coord: 4..363
IPR017451F-box associated interaction domainTIGRFAMTIGR01640TIGR01640coord: 105..332
e-value: 9.4E-28
score: 95.1
IPR011043Galactose oxidase/kelch, beta-propellerSUPERFAMILY50965Galactose oxidase, central domaincoord: 102..297
IPR036047F-box-like domain superfamilySUPERFAMILY81383F-box domaincoord: 4..48

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0031534.1Lag0031534.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005515 protein binding