Lag0031521 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0031521
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	ethylene-responsive transcription factor RAP2-3-like
Location	chr11: 9559818 .. 9560992 (+)
RNA-Seq Expression	Lag0031521
Synteny	Lag0031521

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTGCGGTGGTGCCATCATCTCTGGTTTTATTGAAAAGAAGCGCAGTCGGAAGCTTACTAATGGATCTCTCTGGTCCGACATCGACCCTTTTTCCGACCTCCTCGGCCTCGATTATTCCAATGGTATTTATCTCTTCGTTTTTTTTTCAACTTTTTATTTTTTAAAATCATTATTACCTGAGTATCGAATCATGGAGTAGAGTTAGAAATTTTTTAGAAGAGGGGTAGAATTTTTAATTTTTTTTTAATGTGTAATGGGACCAGCAGATTAGAAAAAAAAATTACAAATTTCTTTAATTTTTCATTGAGTTAGGCAATTGCTTTGATTTCTTTTCTTCTTCTTTTCATTAATGAATAAGTATACGTGTTTTATCTACTTTATAGGATCAAAATCAATTTGTTCATATTTTATTCCAATGGTTTGAAAAAAAAATGATTCCTATTTTAAAATGGTTATTTTACAGAGAGAATAAAAAATTAGTGTACTGGATTTGATTTTTTAAAAAAAAAAATCCAGAAGGGAATGGAAATTTCGATCGTGATCGGTATTGAACTGTGATTGGTTTACTGGTTTGTAGTGAGAAGTGAGAAACCTCAGAAAAGAGAACCAGCTACGGCGGAGACGGCGGCGGCCCCGCCTCTGCCGCCGGAGAAGGCTCGAAAAGCTCGGAAAAATATGTACAGAGGAATCCGGCAGCGGCCGTGGGGAAAATGGGCGGCGGAGATTCGGGACCCACATAAAGGCGTCCGAGTTTGGCTCGGCACTTTCAACACCGCCGAGGAAGCCGCCAGAGCCTACGATGAGGCGGCGAAAAGGATCCGAGGAGAAAAGGCAAAACTCAACTTCGCTCCTCCGCCGCCGTCGCCGCCGTTACTGGAGGCTCAGCCTCAAATCCCGGCTAAAAAACGCTGCGTCCTTCCCGAACAGACTGGGGATTGTTTCAGTTCCACCGGCCCACCGCCGCCTGGCACACGTGCGAACAACTCGTGTGAGAACGACGGGTTCTACTACGGCGAGGAATGGGCGAGTTTGAGATCCTTCCTGGGTCTGGATTGTGAAGAGCAACAACCGAGTCAACTCGGTGGAAGTGACGAGTTTGACCGAGTTGCTGAGGACCTGTGGATGCTGGACGACATGTCGTTGAATCAGCAGAAATTCAGTCAAATGGTGTAG

mRNA sequence

ATGTGCGGTGGTGCCATCATCTCTGGTTTTATTGAAAAGAAGCGCAGTCGGAAGCTTACTAATGGATCTCTCTGGTCCGACATCGACCCTTTTTCCGACCTCCTCGGCCTCGATTATTCCAATGTGAGAAGTGAGAAACCTCAGAAAAGAGAACCAGCTACGGCGGAGACGGCGGCGGCCCCGCCTCTGCCGCCGGAGAAGGCTCGAAAAGCTCGGAAAAATATGTACAGAGGAATCCGGCAGCGGCCGTGGGGAAAATGGGCGGCGGAGATTCGGGACCCACATAAAGGCGTCCGAGTTTGGCTCGGCACTTTCAACACCGCCGAGGAAGCCGCCAGAGCCTACGATGAGGCGGCGAAAAGGATCCGAGGAGAAAAGGCAAAACTCAACTTCGCTCCTCCGCCGCCGTCGCCGCCGTTACTGGAGGCTCAGCCTCAAATCCCGGCTAAAAAACGCTGCGTCCTTCCCGAACAGACTGGGGATTGTTTCAGTTCCACCGGCCCACCGCCGCCTGGCACACGTGCGAACAACTCGTGTGAGAACGACGGGTTCTACTACGGCGAGGAATGGGCGAGTTTGAGATCCTTCCTGGGTCTGGATTGTGAAGAGCAACAACCGAGTCAACTCGGTGGAAGTGACGAGTTTGACCGAGTTGCTGAGGACCTGTGGATGCTGGACGACATGTCGTTGAATCAGCAGAAATTCAGTCAAATGGTGTAG

Coding sequence (CDS)

ATGTGCGGTGGTGCCATCATCTCTGGTTTTATTGAAAAGAAGCGCAGTCGGAAGCTTACTAATGGATCTCTCTGGTCCGACATCGACCCTTTTTCCGACCTCCTCGGCCTCGATTATTCCAATGTGAGAAGTGAGAAACCTCAGAAAAGAGAACCAGCTACGGCGGAGACGGCGGCGGCCCCGCCTCTGCCGCCGGAGAAGGCTCGAAAAGCTCGGAAAAATATGTACAGAGGAATCCGGCAGCGGCCGTGGGGAAAATGGGCGGCGGAGATTCGGGACCCACATAAAGGCGTCCGAGTTTGGCTCGGCACTTTCAACACCGCCGAGGAAGCCGCCAGAGCCTACGATGAGGCGGCGAAAAGGATCCGAGGAGAAAAGGCAAAACTCAACTTCGCTCCTCCGCCGCCGTCGCCGCCGTTACTGGAGGCTCAGCCTCAAATCCCGGCTAAAAAACGCTGCGTCCTTCCCGAACAGACTGGGGATTGTTTCAGTTCCACCGGCCCACCGCCGCCTGGCACACGTGCGAACAACTCGTGTGAGAACGACGGGTTCTACTACGGCGAGGAATGGGCGAGTTTGAGATCCTTCCTGGGTCTGGATTGTGAAGAGCAACAACCGAGTCAACTCGGTGGAAGTGACGAGTTTGACCGAGTTGCTGAGGACCTGTGGATGCTGGACGACATGTCGTTGAATCAGCAGAAATTCAGTCAAATGGTGTAG

Protein sequence

MCGGAIISGFIEKKRSRKLTNGSLWSDIDPFSDLLGLDYSNVRSEKPQKREPATAETAAAPPLPPEKARKARKNMYRGIRQRPWGKWAAEIRDPHKGVRVWLGTFNTAEEAARAYDEAAKRIRGEKAKLNFAPPPPSPPLLEAQPQIPAKKRCVLPEQTGDCFSSTGPPPPGTRANNSCENDGFYYGEEWASLRSFLGLDCEEQQPSQLGGSDEFDRVAEDLWMLDDMSLNQQKFSQMV

Homology

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BLAST of Lag0031521 vs. NCBI nr
Match: XP_008458405.1 (PREDICTED: ethylene-responsive transcription factor RAP2-3-like [Cucumis melo])

HSP 1 Score: 339.0 bits (868), Expect = 3.4e-89
Identity = 175/239 (73.22%), Postives = 200/239 (83.68%), Query Frame = 0


Query: 1   MCGGAIISGFIEKKRSRKLTNGSLWSDIDPFSDLLGLDYSNVRSEKPQKREPATAETAAA 60
           MCGGAIIS FIEK+R+ KL N SLWS IDPFSDLLGL+YS+V++EKP+KR+ +TA  A A
Sbjct: 20  MCGGAIISDFIEKRRTCKLNNESLWSHIDPFSDLLGLNYSSVKTEKPKKRDRSTAAEAVA 79

Query: 61  PPLPPEKARKARKNMYRGIRQRPWGKWAAEIRDPHKGVRVWLGTFNTAEEAARAYDEAAK 120
             +  EK RK RKN+YRGIRQRPWGKWAAEIRDP KGVRVWLGTFNTAEEAARAYDEAAK
Sbjct: 80  --VAVEKTRKVRKNLYRGIRQRPWGKWAAEIRDPRKGVRVWLGTFNTAEEAARAYDEAAK 139

Query: 121 RIRGEKAKLNFAPPPPSPPLLEAQPQIPAKKRCVLPEQTGDCFSSTGPPPPGTRANNSCE 180
           RIRG KAKLNFAP PPSP    AQPQ P KK+CVLPE T   F++T   PP TRA++SC+
Sbjct: 140 RIRGVKAKLNFAPAPPSP---TAQPQTPVKKQCVLPELTPGGFNTT-DSPPCTRADSSCD 199

Query: 181 NDGFYYGEEWASLRSFLGLDCEEQQPSQLGGSDEFDRVAEDLWMLDDMSLNQQKFSQMV 240
           ++  YYGEE ASLRSFLGLD EEQQPSQLGG+ EF+RV  DLWM+DDMSLN++KFSQ+V
Sbjct: 200 SEELYYGEELASLRSFLGLDGEEQQPSQLGGNGEFERVDLDLWMMDDMSLNEEKFSQLV 252

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BLAST of Lag0031521 vs. NCBI nr
Match: XP_023000151.1 (ethylene-responsive transcription factor RAP2-3 [Cucurbita maxima])

HSP 1 Score: 337.4 bits (864), Expect = 9.9e-89
Identity = 180/240 (75.00%), Postives = 194/240 (80.83%), Query Frame = 0


Query: 1   MCGGAIISGFIEKKRSRKLTNGSLWSDIDPFSDLLGLDYSNVRSEKPQKREPATAETAAA 60
           MCGGA+ISG IEKKR RKL+NGSLW D D FSDLLGLDY  +++E PQK+  ATAETAA 
Sbjct: 1   MCGGALISGIIEKKRGRKLSNGSLWPDTDHFSDLLGLDYCALKTETPQKKCRATAETAAV 60

Query: 61  PPLPPEKARKARKNMYRGIRQRPWGKWAAEIRDPHKGVRVWLGTFNTAEEAARAYDEAAK 120
           P   PEK RKARKN+YRGIRQRPWGKWAAEIRDP KGVRVWLGTFNTAEEAARAYDEAAK
Sbjct: 61  P--EPEKTRKARKNIYRGIRQRPWGKWAAEIRDPQKGVRVWLGTFNTAEEAARAYDEAAK 120

Query: 121 RIRGEKAKLNFAPPPPSPPLLEAQPQIPAKKRCVLPEQTGDCFSSTGPPPPGTRANNSCE 180
           RIRGEKAKLNFAP P   PL  +QP  PAKKRCV  E T DCFSST   P  TR NNSCE
Sbjct: 121 RIRGEKAKLNFAPRP--QPLPVSQP--PAKKRCVAHELTPDCFSSTSSAPASTRVNNSCE 180

Query: 181 NDGFYYGEEWASLRSFLGLDC-EEQQPSQLGGSDEFDRVAEDLWMLDDMSLNQQKFSQMV 240
            DG YYGEE ASLRSFL LD  EEQQPSQ+GGSDEF +  E LWM+DD SLN++KFS +V
Sbjct: 181 IDGLYYGEELASLRSFLDLDFEEEQQPSQVGGSDEFGQAVE-LWMMDDPSLNEEKFSSVV 233

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BLAST of Lag0031521 vs. NCBI nr
Match: KGN47418.1 (hypothetical protein Csa_023005 [Cucumis sativus])

HSP 1 Score: 331.3 bits (848), Expect = 7.1e-87
Identity = 173/239 (72.38%), Postives = 196/239 (82.01%), Query Frame = 0


Query: 1   MCGGAIISGFIEKKRSRKLTNGSLWSDIDPFSDLLGLDYSNVRSEKPQKREPATAETAAA 60
           MCGGAIIS FIEKKR+ KL N SLWS IDPFSDLLGL+YS+V++E P+KR  +TA  A A
Sbjct: 1   MCGGAIISDFIEKKRTCKLNNESLWSHIDPFSDLLGLNYSSVKTETPKKRNRSTAAEAVA 60

Query: 61  PPLPPEKARKARKNMYRGIRQRPWGKWAAEIRDPHKGVRVWLGTFNTAEEAARAYDEAAK 120
                EK RKARKN+YRGIRQRPWGKWAAEIRDP KGVRVWLGTFNTAEEAARAYDEAAK
Sbjct: 61  ----VEKTRKARKNIYRGIRQRPWGKWAAEIRDPRKGVRVWLGTFNTAEEAARAYDEAAK 120

Query: 121 RIRGEKAKLNFAPPPPSPPLLEAQPQIPAKKRCVLPEQTGDCFSSTGPPPPGTRANNSCE 180
           RIRGEKAKLNFAP PPS   L AQ Q P K +CV+PE T    ++TG  PP TRA++SC+
Sbjct: 121 RIRGEKAKLNFAPAPPS---LAAQRQTPVKMQCVVPEFTPSGLNTTG-SPPCTRADSSCD 180

Query: 181 NDGFYYGEEWASLRSFLGLDCEEQQPSQLGGSDEFDRVAEDLWMLDDMSLNQQKFSQMV 240
           ++  YYGEE ASLRSFLGLD EEQQ S+LGG+ EF+RV  D+WM+DDMSLN+QKFSQMV
Sbjct: 181 SEELYYGEELASLRSFLGLDSEEQQASELGGNGEFERVDLDVWMMDDMSLNEQKFSQMV 231

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BLAST of Lag0031521 vs. NCBI nr
Match: XP_004153075.3 (ethylene-responsive transcription factor RAP2-3 [Cucumis sativus])

HSP 1 Score: 331.3 bits (848), Expect = 7.1e-87
Identity = 173/239 (72.38%), Postives = 196/239 (82.01%), Query Frame = 0


Query: 1   MCGGAIISGFIEKKRSRKLTNGSLWSDIDPFSDLLGLDYSNVRSEKPQKREPATAETAAA 60
           MCGGAIIS FIEKKR+ KL N SLWS IDPFSDLLGL+YS+V++E P+KR  +TA  A A
Sbjct: 20  MCGGAIISDFIEKKRTCKLNNESLWSHIDPFSDLLGLNYSSVKTETPKKRNRSTAAEAVA 79

Query: 61  PPLPPEKARKARKNMYRGIRQRPWGKWAAEIRDPHKGVRVWLGTFNTAEEAARAYDEAAK 120
                EK RKARKN+YRGIRQRPWGKWAAEIRDP KGVRVWLGTFNTAEEAARAYDEAAK
Sbjct: 80  ----VEKTRKARKNIYRGIRQRPWGKWAAEIRDPRKGVRVWLGTFNTAEEAARAYDEAAK 139

Query: 121 RIRGEKAKLNFAPPPPSPPLLEAQPQIPAKKRCVLPEQTGDCFSSTGPPPPGTRANNSCE 180
           RIRGEKAKLNFAP PPS   L AQ Q P K +CV+PE T    ++TG  PP TRA++SC+
Sbjct: 140 RIRGEKAKLNFAPAPPS---LAAQRQTPVKMQCVVPEFTPSGLNTTG-SPPCTRADSSCD 199

Query: 181 NDGFYYGEEWASLRSFLGLDCEEQQPSQLGGSDEFDRVAEDLWMLDDMSLNQQKFSQMV 240
           ++  YYGEE ASLRSFLGLD EEQQ S+LGG+ EF+RV  D+WM+DDMSLN+QKFSQMV
Sbjct: 200 SEELYYGEELASLRSFLGLDSEEQQASELGGNGEFERVDLDVWMMDDMSLNEQKFSQMV 250

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BLAST of Lag0031521 vs. NCBI nr
Match: XP_038876065.1 (ethylene-responsive transcription factor RAP2-3-like [Benincasa hispida])

HSP 1 Score: 328.6 bits (841), Expect = 4.6e-86
Identity = 171/240 (71.25%), Postives = 192/240 (80.00%), Query Frame = 0


Query: 1   MCGGAIISGFIEKKRSRKLTNGSLWSDIDPFSDLLGLDYSNVRSEKPQKREPATAETAAA 60
           MCGGAIIS FIEKKR+RKLTN SLWS IDPFSDLL L+YS V++E+P+KR+ ATA  A A
Sbjct: 45  MCGGAIISDFIEKKRTRKLTNESLWSHIDPFSDLLALNYSPVKTEEPKKRDRATA--AEA 104

Query: 61  PPLPPEKARKARKNMYRGIRQRPWGKWAAEIRDPHKGVRVWLGTFNTAEEAARAYDEAAK 120
             +  EK  K RKN+YRGIRQRPWGKWAAEIRDP KGVRVWLGTFNTAEEAARAYDEAAK
Sbjct: 105 VGVAVEKTPKTRKNLYRGIRQRPWGKWAAEIRDPQKGVRVWLGTFNTAEEAARAYDEAAK 164

Query: 121 RIRGEKAKLNFAPPPPSP-PLLEAQPQIPAKKRCVLPEQTGDCFSSTGPPPPGTRANNSC 180
           RIRGEKAKLNFAP PPS  PL  AQ Q P KKRCV+PE T   F++TGPPP        C
Sbjct: 165 RIRGEKAKLNFAPAPPSSRPLPAAQLQSPVKKRCVVPESTRGGFNTTGPPP--------C 224

Query: 181 ENDGFYYGEEWASLRSFLGLDCEEQQPSQLGGSDEFDRVAEDLWMLDDMSLNQQKFSQMV 240
           ++D FYYG+E ASLRSFLGLDCEEQQP QL G+ E + V    WM+DDMS+N+QKFSQ+V
Sbjct: 225 DSDEFYYGQELASLRSFLGLDCEEQQPCQLSGNGELEPVDVGFWMMDDMSMNEQKFSQLV 274

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BLAST of Lag0031521 vs. ExPASy Swiss-Prot
Match: P42736 (Ethylene-responsive transcription factor RAP2-3 OS=Arabidopsis thaliana OX=3702 GN=RAP2-3 PE=1 SV=2)

HSP 1 Score: 167.2 bits (422), Expect = 2.3e-40
Identity = 118/254 (46.46%), Postives = 146/254 (57.48%), Query Frame = 0


Query: 1   MCGGAIISGF---IEKKRSRKLTNGSLWSDIDPFS--DLLGLDYSNVR---SEKPQKREP 60
           MCGGAIIS +   + K + RKLT   LWS++D  +  D  G  YS  +   + +   +E 
Sbjct: 1   MCGGAIISDYAPLVTKAKGRKLTAEELWSELDASAADDFWGF-YSTSKLHPTNQVNVKEE 60

Query: 61  ATAETAAAPPLPPEKARKARKNMYRGIRQRPWGKWAAEIRDPHKGVRVWLGTFNTAEEAA 120
           A  +  A  P      R+ RKN+YRGIR+RPWGKWAAEIRDP KGVRVWLGTFNTAEEAA
Sbjct: 61  AVKKEQATEP----GKRRKRKNVYRGIRKRPWGKWAAEIRDPRKGVRVWLGTFNTAEEAA 120

Query: 121 RAYDEAAKRIRGEKAKLNFA----PPPPS----PPLLEAQPQIPAKKRCVLPEQTGDCFS 180
            AYD AAK+IRG+KAKLNF     PPPP+    P    +  Q PAKK CV+ +   +  S
Sbjct: 121 MAYDVAAKQIRGDKAKLNFPDLHHPPPPNYTPPPSSPRSTDQPPAKKVCVVSQSESE-LS 180

Query: 181 STGPPPPGTRANNSCENDGFYYG--------EEWASLRSFLGLDCE-EQQPSQLGGSDEF 229
               P       N  E     YG        ++ +SL SFL LD    +QPSQL  S   
Sbjct: 181 QPSFPVECIGFGNGDEFQNLSYGFEPDYDLKQQISSLESFLELDGNTAEQPSQLDES--- 240

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BLAST of Lag0031521 vs. ExPASy Swiss-Prot
Match: O22259 (Ethylene-responsive transcription factor ERF071 OS=Arabidopsis thaliana OX=3702 GN=ERF071 PE=1 SV=1)

HSP 1 Score: 122.1 bits (305), Expect = 8.6e-27
Identity = 72/131 (54.96%), Postives = 81/131 (61.83%), Query Frame = 0


Query: 1   MCGGAIISGFIEKKRSRKLTNGSLWSDIDPFSDLLGLDYSNVRSEKPQKREPATAETAAA 60
           MCGGAIIS FI             WS  +     LG    +V S K  KR+P +      
Sbjct: 1   MCGGAIISDFI-------------WSKSESEPSQLG----SVSSRK--KRKPVSVS---- 60

Query: 61  PPLPPEKARKARKNMYRGIRQRPWGKWAAEIRDPHKGVRVWLGTFNTAEEAARAYDEAAK 120
                   ++ RKN+YRGIRQRPWGKWAAEIRDP KGVRVWLGTF TA+EAARAYD AA 
Sbjct: 61  ---EERDGKRERKNLYRGIRQRPWGKWAAEIRDPSKGVRVWLGTFKTADEAARAYDVAAI 105

Query: 121 RIRGEKAKLNF 132
           +IRG KAKLNF
Sbjct: 121 KIRGRKAKLNF 105

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BLAST of Lag0031521 vs. ExPASy Swiss-Prot
Match: Q9SSA8 (Ethylene-responsive transcription factor RAP2-12 OS=Arabidopsis thaliana OX=3702 GN=RAP2-12 PE=1 SV=1)

HSP 1 Score: 121.3 bits (303), Expect = 1.5e-26
Identity = 75/180 (41.67%), Postives = 90/180 (50.00%), Query Frame = 0


Query: 1   MCGGAIISGFIEKKRSRKLTNGSLW----------------------------SDIDPFS 60
           MCGGAIIS FI   RSR++T+  +W                            +D   F 
Sbjct: 1   MCGGAIISDFIPPPRSRRVTSEFIWPDLKKNLKGSKKSSKNRSNFFDFDAEFEADFQGFK 60

Query: 61  DLLGLDYSN--------------VRSEKPQKREPATAE-------TAAAPPLPPEKARKA 120
           D   +D  +              V +  P+    A AE                + A + 
Sbjct: 61  DDSSIDCDDDFDVGDVFADVKPFVFTSTPKPAVSAAAEGSVFGKKVTGLDGDAEKSANRK 120

Query: 121 RKNMYRGIRQRPWGKWAAEIRDPHKGVRVWLGTFNTAEEAARAYDEAAKRIRGEKAKLNF 132
           RKN YRGIRQRPWGKWAAEIRDP +G R+WLGTF TAEEAARAYD AA+RIRG KAK+NF
Sbjct: 121 RKNQYRGIRQRPWGKWAAEIRDPREGARIWLGTFKTAEEAARAYDAAARRIRGSKAKVNF 180

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BLAST of Lag0031521 vs. ExPASy Swiss-Prot
Match: Q8H0T5 (Ethylene-responsive transcription factor ERF073 OS=Arabidopsis thaliana OX=3702 GN=ERF073 PE=1 SV=2)

HSP 1 Score: 118.2 bits (295), Expect = 1.2e-25
Identity = 67/135 (49.63%), Postives = 85/135 (62.96%), Query Frame = 0


Query: 1   MCGGAIISGFIEKKRSRKLTNGSLWSDIDPFS----DLLGLDYSNVRSEKPQKREPATAE 60
           MCGGA+IS +I  ++  + +  S W     F     D  G ++  + S++P         
Sbjct: 1   MCGGAVISDYIAPEKIARSSGKSSWRSNGVFDCSIYDFDG-NFDELESDEPFVFSSTHKH 60

Query: 61  TAAAPPLPPEKARKARKNMYRGIRQRPWGKWAAEIRDPHKGVRVWLGTFNTAEEAARAYD 120
            A+      +K + +R   Y+GIR+RPWG+WAAEIRDP KGVRVWLGTFNTAEEAARAYD
Sbjct: 61  HASGSASDGKKKQSSR---YKGIRRRPWGRWAAEIRDPIKGVRVWLGTFNTAEEAARAYD 120

Query: 121 EAAKRIRGEKAKLNF 132
             AKRIRG KAKLNF
Sbjct: 121 LEAKRIRGAKAKLNF 131

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BLAST of Lag0031521 vs. ExPASy Swiss-Prot
Match: Q9LUM4 (Ethylene-responsive transcription factor RAP2-2 OS=Arabidopsis thaliana OX=3702 GN=RAP2-2 PE=1 SV=2)

HSP 1 Score: 118.2 bits (295), Expect = 1.2e-25
Identity = 81/202 (40.10%), Postives = 101/202 (50.00%), Query Frame = 0


Query: 1   MCGGAIISGFIEKKRSRKLTNGSLWSDI-----------DPFSDLLGLD----------- 60
           MCGGAIIS FI   RS ++TN  +W D+           +  SD   LD           
Sbjct: 1   MCGGAIISDFIPPPRSLRVTNEFIWPDLKNKVKASKKRSNKRSDFFDLDDDFEADFQGFK 60

Query: 61  -------------YSNVR------SEKPQKR-----------EPATAETAAAPPLPPEKA 120
                        + NV+      + KP                   +T  +     + +
Sbjct: 61  DDSAFDCEDDDDVFVNVKPFVFTATTKPVASAFVSTGIYLVGSAYAKKTVESAEQAEKSS 120

Query: 121 RKARKNMYRGIRQRPWGKWAAEIRDPHKGVRVWLGTFNTAEEAARAYDEAAKRIRGEKAK 151
           ++ RKN YRGIRQRPWGKWAAEIRDP KG R WLGTF+TAEEAARAYD AA+RIRG KAK
Sbjct: 121 KRKRKNQYRGIRQRPWGKWAAEIRDPRKGSREWLGTFDTAEEAARAYDAAARRIRGTKAK 180

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BLAST of Lag0031521 vs. ExPASy TrEMBL
Match: A0A1S3C7W9 (ethylene-responsive transcription factor RAP2-3-like OS=Cucumis melo OX=3656 GN=LOC103497827 PE=4 SV=1)

HSP 1 Score: 339.0 bits (868), Expect = 1.6e-89
Identity = 175/239 (73.22%), Postives = 200/239 (83.68%), Query Frame = 0


Query: 1   MCGGAIISGFIEKKRSRKLTNGSLWSDIDPFSDLLGLDYSNVRSEKPQKREPATAETAAA 60
           MCGGAIIS FIEK+R+ KL N SLWS IDPFSDLLGL+YS+V++EKP+KR+ +TA  A A
Sbjct: 20  MCGGAIISDFIEKRRTCKLNNESLWSHIDPFSDLLGLNYSSVKTEKPKKRDRSTAAEAVA 79

Query: 61  PPLPPEKARKARKNMYRGIRQRPWGKWAAEIRDPHKGVRVWLGTFNTAEEAARAYDEAAK 120
             +  EK RK RKN+YRGIRQRPWGKWAAEIRDP KGVRVWLGTFNTAEEAARAYDEAAK
Sbjct: 80  --VAVEKTRKVRKNLYRGIRQRPWGKWAAEIRDPRKGVRVWLGTFNTAEEAARAYDEAAK 139

Query: 121 RIRGEKAKLNFAPPPPSPPLLEAQPQIPAKKRCVLPEQTGDCFSSTGPPPPGTRANNSCE 180
           RIRG KAKLNFAP PPSP    AQPQ P KK+CVLPE T   F++T   PP TRA++SC+
Sbjct: 140 RIRGVKAKLNFAPAPPSP---TAQPQTPVKKQCVLPELTPGGFNTT-DSPPCTRADSSCD 199

Query: 181 NDGFYYGEEWASLRSFLGLDCEEQQPSQLGGSDEFDRVAEDLWMLDDMSLNQQKFSQMV 240
           ++  YYGEE ASLRSFLGLD EEQQPSQLGG+ EF+RV  DLWM+DDMSLN++KFSQ+V
Sbjct: 200 SEELYYGEELASLRSFLGLDGEEQQPSQLGGNGEFERVDLDLWMMDDMSLNEEKFSQLV 252

[Close]

BLAST of Lag0031521 vs. ExPASy TrEMBL
Match: A0A6J1KF31 (ethylene-responsive transcription factor RAP2-3 OS=Cucurbita maxima OX=3661 GN=LOC111494435 PE=4 SV=1)

HSP 1 Score: 337.4 bits (864), Expect = 4.8e-89
Identity = 180/240 (75.00%), Postives = 194/240 (80.83%), Query Frame = 0


Query: 1   MCGGAIISGFIEKKRSRKLTNGSLWSDIDPFSDLLGLDYSNVRSEKPQKREPATAETAAA 60
           MCGGA+ISG IEKKR RKL+NGSLW D D FSDLLGLDY  +++E PQK+  ATAETAA 
Sbjct: 1   MCGGALISGIIEKKRGRKLSNGSLWPDTDHFSDLLGLDYCALKTETPQKKCRATAETAAV 60

Query: 61  PPLPPEKARKARKNMYRGIRQRPWGKWAAEIRDPHKGVRVWLGTFNTAEEAARAYDEAAK 120
           P   PEK RKARKN+YRGIRQRPWGKWAAEIRDP KGVRVWLGTFNTAEEAARAYDEAAK
Sbjct: 61  P--EPEKTRKARKNIYRGIRQRPWGKWAAEIRDPQKGVRVWLGTFNTAEEAARAYDEAAK 120

Query: 121 RIRGEKAKLNFAPPPPSPPLLEAQPQIPAKKRCVLPEQTGDCFSSTGPPPPGTRANNSCE 180
           RIRGEKAKLNFAP P   PL  +QP  PAKKRCV  E T DCFSST   P  TR NNSCE
Sbjct: 121 RIRGEKAKLNFAPRP--QPLPVSQP--PAKKRCVAHELTPDCFSSTSSAPASTRVNNSCE 180

Query: 181 NDGFYYGEEWASLRSFLGLDC-EEQQPSQLGGSDEFDRVAEDLWMLDDMSLNQQKFSQMV 240
            DG YYGEE ASLRSFL LD  EEQQPSQ+GGSDEF +  E LWM+DD SLN++KFS +V
Sbjct: 181 IDGLYYGEELASLRSFLDLDFEEEQQPSQVGGSDEFGQAVE-LWMMDDPSLNEEKFSSVV 233

[Close]

BLAST of Lag0031521 vs. ExPASy TrEMBL
Match: A0A0A0KD18 (AP2/ERF domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G318160 PE=4 SV=1)

HSP 1 Score: 331.3 bits (848), Expect = 3.4e-87
Identity = 173/239 (72.38%), Postives = 196/239 (82.01%), Query Frame = 0


Query: 1   MCGGAIISGFIEKKRSRKLTNGSLWSDIDPFSDLLGLDYSNVRSEKPQKREPATAETAAA 60
           MCGGAIIS FIEKKR+ KL N SLWS IDPFSDLLGL+YS+V++E P+KR  +TA  A A
Sbjct: 1   MCGGAIISDFIEKKRTCKLNNESLWSHIDPFSDLLGLNYSSVKTETPKKRNRSTAAEAVA 60

Query: 61  PPLPPEKARKARKNMYRGIRQRPWGKWAAEIRDPHKGVRVWLGTFNTAEEAARAYDEAAK 120
                EK RKARKN+YRGIRQRPWGKWAAEIRDP KGVRVWLGTFNTAEEAARAYDEAAK
Sbjct: 61  ----VEKTRKARKNIYRGIRQRPWGKWAAEIRDPRKGVRVWLGTFNTAEEAARAYDEAAK 120

Query: 121 RIRGEKAKLNFAPPPPSPPLLEAQPQIPAKKRCVLPEQTGDCFSSTGPPPPGTRANNSCE 180
           RIRGEKAKLNFAP PPS   L AQ Q P K +CV+PE T    ++TG  PP TRA++SC+
Sbjct: 121 RIRGEKAKLNFAPAPPS---LAAQRQTPVKMQCVVPEFTPSGLNTTG-SPPCTRADSSCD 180

Query: 181 NDGFYYGEEWASLRSFLGLDCEEQQPSQLGGSDEFDRVAEDLWMLDDMSLNQQKFSQMV 240
           ++  YYGEE ASLRSFLGLD EEQQ S+LGG+ EF+RV  D+WM+DDMSLN+QKFSQMV
Sbjct: 181 SEELYYGEELASLRSFLGLDSEEQQASELGGNGEFERVDLDVWMMDDMSLNEQKFSQMV 231

[Close]

BLAST of Lag0031521 vs. ExPASy TrEMBL
Match: A0A6J1ER95 (ethylene-responsive transcription factor RAP2-3 OS=Cucurbita moschata OX=3662 GN=LOC111436844 PE=4 SV=1)

HSP 1 Score: 322.0 bits (824), Expect = 2.1e-84
Identity = 172/240 (71.67%), Postives = 187/240 (77.92%), Query Frame = 0


Query: 1   MCGGAIISGFIEKKRSRKLTNGSLWSDIDPFSDLLGLDYSNVRSEKPQKREPATAETAAA 60
           MCGG++ISG IEKKR RKL NGSLW D D FSDLLGLDY  +++E  QK   ATA     
Sbjct: 1   MCGGSLISGIIEKKRGRKLRNGSLWPDTDHFSDLLGLDYCALKTETSQKECRATAVP--- 60

Query: 61  PPLPPEKARKARKNMYRGIRQRPWGKWAAEIRDPHKGVRVWLGTFNTAEEAARAYDEAAK 120
               PEK RKARKN+YRGIRQRPWGKWAAEIRDP KGVRVWLGTFNTAEEAARAYDEAAK
Sbjct: 61  ---EPEKTRKARKNIYRGIRQRPWGKWAAEIRDPQKGVRVWLGTFNTAEEAARAYDEAAK 120

Query: 121 RIRGEKAKLNFAPPPPSPPLLEAQPQIPAKKRCVLPEQTGDCFSSTGPPPPGTRANNSCE 180
           RIRGEKAKLNFAP P   PL  +QP  PAKKRCV  E T DCFSST P P  TR NNSCE
Sbjct: 121 RIRGEKAKLNFAPRP--QPLPVSQP--PAKKRCVAHELTSDCFSSTSPAPASTRVNNSCE 180

Query: 181 NDGFYYGEEWASLRSFLGLDC-EEQQPSQLGGSDEFDRVAEDLWMLDDMSLNQQKFSQMV 240
            DG YYGEE ASLRSFL LD  EEQ+PSQ+GGSDEF +  E LWM+DD SLN++KF ++V
Sbjct: 181 IDGLYYGEELASLRSFLDLDFEEEQEPSQVGGSDEFGQAVE-LWMMDDPSLNEEKFGEVV 229

[Close]

BLAST of Lag0031521 vs. ExPASy TrEMBL
Match: A0A6J1CA63 (ethylene-responsive transcription factor RAP2-3-like OS=Momordica charantia OX=3673 GN=LOC111009718 PE=4 SV=1)

HSP 1 Score: 320.1 bits (819), Expect = 7.9e-84
Identity = 173/249 (69.48%), Postives = 192/249 (77.11%), Query Frame = 0


Query: 1   MCGGAIISGFIEKKRSRKLTNGSLWSDIDPFSDLLGLDYSNVRSEKPQKREPATAETAAA 60
           MCGGAIIS FIEKKRSRK +NG LWS+IDPFSDLLGLD+S+V+SEKPQKRE + A  A  
Sbjct: 1   MCGGAIISDFIEKKRSRKFSNGDLWSEIDPFSDLLGLDFSSVKSEKPQKRERSPAVAAEG 60

Query: 61  PPLPPEKARKARKNMYRGIRQRPWGKWAAEIRDPHKGVRVWLGTFNTAEEAARAYDEAAK 120
             L P   RK RKN+YRGIRQRPWGKWAAEIRDP KGVRVWLGTF+TAEEAARAYDEAAK
Sbjct: 61  IELKP---RKTRKNLYRGIRQRPWGKWAAEIRDPQKGVRVWLGTFSTAEEAARAYDEAAK 120

Query: 121 RIRGEKAKLNFAPPPPSPPLLEAQPQIPAKKRCVLPEQTGDCFSSTGPPPPGTRANNSCE 180
           RIRGEKAKLNFAP         AQPQ PAKKRCVLPE T +  ++TG PP  +R +NS  
Sbjct: 121 RIRGEKAKLNFAP--------AAQPQAPAKKRCVLPELTREGLNTTG-PPTRSRTSNS-Y 180

Query: 181 NDGFYYGEEWAS----------LRSFLGLDCEEQQPSQLGGSDEFDRVAEDLWMLDDMSL 240
           NDG YYG+E AS          L SFLGLD E+QQ SQLGGSDEFDRV  DLWM+DD SL
Sbjct: 181 NDGIYYGDELASGFELKEQISNLTSFLGLDGEDQQLSQLGGSDEFDRVVVDLWMMDDRSL 236

[Close]

BLAST of Lag0031521 vs. TAIR 10
Match: AT3G16770.1 (ethylene-responsive element binding protein )

HSP 1 Score: 167.2 bits (422), Expect = 1.7e-41
Identity = 118/254 (46.46%), Postives = 146/254 (57.48%), Query Frame = 0


Query: 1   MCGGAIISGF---IEKKRSRKLTNGSLWSDIDPFS--DLLGLDYSNVR---SEKPQKREP 60
           MCGGAIIS +   + K + RKLT   LWS++D  +  D  G  YS  +   + +   +E 
Sbjct: 1   MCGGAIISDYAPLVTKAKGRKLTAEELWSELDASAADDFWGF-YSTSKLHPTNQVNVKEE 60

Query: 61  ATAETAAAPPLPPEKARKARKNMYRGIRQRPWGKWAAEIRDPHKGVRVWLGTFNTAEEAA 120
           A  +  A  P      R+ RKN+YRGIR+RPWGKWAAEIRDP KGVRVWLGTFNTAEEAA
Sbjct: 61  AVKKEQATEP----GKRRKRKNVYRGIRKRPWGKWAAEIRDPRKGVRVWLGTFNTAEEAA 120

Query: 121 RAYDEAAKRIRGEKAKLNFA----PPPPS----PPLLEAQPQIPAKKRCVLPEQTGDCFS 180
            AYD AAK+IRG+KAKLNF     PPPP+    P    +  Q PAKK CV+ +   +  S
Sbjct: 121 MAYDVAAKQIRGDKAKLNFPDLHHPPPPNYTPPPSSPRSTDQPPAKKVCVVSQSESE-LS 180

Query: 181 STGPPPPGTRANNSCENDGFYYG--------EEWASLRSFLGLDCE-EQQPSQLGGSDEF 229
               P       N  E     YG        ++ +SL SFL LD    +QPSQL  S   
Sbjct: 181 QPSFPVECIGFGNGDEFQNLSYGFEPDYDLKQQISSLESFLELDGNTAEQPSQLDES--- 240

[Close]

BLAST of Lag0031521 vs. TAIR 10
Match: AT1G53910.3 (related to AP2 12 )

HSP 1 Score: 122.5 bits (306), Expect = 4.7e-28
Identity = 75/178 (42.13%), Postives = 90/178 (50.56%), Query Frame = 0


Query: 1   MCGGAIISGFIEKKRSRKLTNGSLW----------------------------SDIDPFS 60
           MCGGAIIS FI   RSR++T+  +W                            +D   F 
Sbjct: 1   MCGGAIISDFIPPPRSRRVTSEFIWPDLKKNLKGSKKSSKNRSNFFDFDAEFEADFQGFK 60

Query: 61  DLLGLDYSN--------------VRSEKPQKREPATAETAAAPPL-----PPEKARKARK 120
           D   +D  +              V +  P+    A AE              + A + RK
Sbjct: 61  DDSSIDCDDDFDVGDVFADVKPFVFTSTPKPAVSAAAEVFGKKVTGLDGDAEKSANRKRK 120

Query: 121 NMYRGIRQRPWGKWAAEIRDPHKGVRVWLGTFNTAEEAARAYDEAAKRIRGEKAKLNF 132
           N YRGIRQRPWGKWAAEIRDP +G R+WLGTF TAEEAARAYD AA+RIRG KAK+NF
Sbjct: 121 NQYRGIRQRPWGKWAAEIRDPREGARIWLGTFKTAEEAARAYDAAARRIRGSKAKVNF 178

[Close]

BLAST of Lag0031521 vs. TAIR 10
Match: AT2G47520.1 (Integrase-type DNA-binding superfamily protein )

HSP 1 Score: 122.1 bits (305), Expect = 6.1e-28
Identity = 72/131 (54.96%), Postives = 81/131 (61.83%), Query Frame = 0


Query: 1   MCGGAIISGFIEKKRSRKLTNGSLWSDIDPFSDLLGLDYSNVRSEKPQKREPATAETAAA 60
           MCGGAIIS FI             WS  +     LG    +V S K  KR+P +      
Sbjct: 1   MCGGAIISDFI-------------WSKSESEPSQLG----SVSSRK--KRKPVSVS---- 60

Query: 61  PPLPPEKARKARKNMYRGIRQRPWGKWAAEIRDPHKGVRVWLGTFNTAEEAARAYDEAAK 120
                   ++ RKN+YRGIRQRPWGKWAAEIRDP KGVRVWLGTF TA+EAARAYD AA 
Sbjct: 61  ---EERDGKRERKNLYRGIRQRPWGKWAAEIRDPSKGVRVWLGTFKTADEAARAYDVAAI 105

Query: 121 RIRGEKAKLNF 132
           +IRG KAKLNF
Sbjct: 121 KIRGRKAKLNF 105

[Close]

BLAST of Lag0031521 vs. TAIR 10
Match: AT1G53910.1 (related to AP2 12 )

HSP 1 Score: 121.3 bits (303), Expect = 1.0e-27
Identity = 75/180 (41.67%), Postives = 90/180 (50.00%), Query Frame = 0


Query: 1   MCGGAIISGFIEKKRSRKLTNGSLW----------------------------SDIDPFS 60
           MCGGAIIS FI   RSR++T+  +W                            +D   F 
Sbjct: 1   MCGGAIISDFIPPPRSRRVTSEFIWPDLKKNLKGSKKSSKNRSNFFDFDAEFEADFQGFK 60

Query: 61  DLLGLDYSN--------------VRSEKPQKREPATAE-------TAAAPPLPPEKARKA 120
           D   +D  +              V +  P+    A AE                + A + 
Sbjct: 61  DDSSIDCDDDFDVGDVFADVKPFVFTSTPKPAVSAAAEGSVFGKKVTGLDGDAEKSANRK 120

Query: 121 RKNMYRGIRQRPWGKWAAEIRDPHKGVRVWLGTFNTAEEAARAYDEAAKRIRGEKAKLNF 132
           RKN YRGIRQRPWGKWAAEIRDP +G R+WLGTF TAEEAARAYD AA+RIRG KAK+NF
Sbjct: 121 RKNQYRGIRQRPWGKWAAEIRDPREGARIWLGTFKTAEEAARAYDAAARRIRGSKAKVNF 180

[Close]

BLAST of Lag0031521 vs. TAIR 10
Match: AT1G53910.2 (related to AP2 12 )

HSP 1 Score: 121.3 bits (303), Expect = 1.0e-27
Identity = 75/180 (41.67%), Postives = 90/180 (50.00%), Query Frame = 0


Query: 1   MCGGAIISGFIEKKRSRKLTNGSLW----------------------------SDIDPFS 60
           MCGGAIIS FI   RSR++T+  +W                            +D   F 
Sbjct: 1   MCGGAIISDFIPPPRSRRVTSEFIWPDLKKNLKGSKKSSKNRSNFFDFDAEFEADFQGFK 60

Query: 61  DLLGLDYSN--------------VRSEKPQKREPATAE-------TAAAPPLPPEKARKA 120
           D   +D  +              V +  P+    A AE                + A + 
Sbjct: 61  DDSSIDCDDDFDVGDVFADVKPFVFTSTPKPAVSAAAEGSVFGKKVTGLDGDAEKSANRK 120

Query: 121 RKNMYRGIRQRPWGKWAAEIRDPHKGVRVWLGTFNTAEEAARAYDEAAKRIRGEKAKLNF 132
           RKN YRGIRQRPWGKWAAEIRDP +G R+WLGTF TAEEAARAYD AA+RIRG KAK+NF
Sbjct: 121 RKNQYRGIRQRPWGKWAAEIRDPREGARIWLGTFKTAEEAARAYDAAARRIRGSKAKVNF 180

The following BLAST results are available for this feature:

BLAST of Lag0031521 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008458405.1	3.4e-89	73.22	PREDICTED: ethylene-responsive transcription factor RAP2-3-like [Cucumis melo]	[more]
XP_023000151.1	9.9e-89	75.00	ethylene-responsive transcription factor RAP2-3 [Cucurbita maxima]	[more]
KGN47418.1	7.1e-87	72.38	hypothetical protein Csa_023005 [Cucumis sativus]	[more]
XP_004153075.3	7.1e-87	72.38	ethylene-responsive transcription factor RAP2-3 [Cucumis sativus]	[more]
XP_038876065.1	4.6e-86	71.25	ethylene-responsive transcription factor RAP2-3-like [Benincasa hispida]	[more]

BLAST of Lag0031521 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P42736	2.3e-40	46.46	Ethylene-responsive transcription factor RAP2-3 OS=Arabidopsis thaliana OX=3702 ...	[more]
O22259	8.6e-27	54.96	Ethylene-responsive transcription factor ERF071 OS=Arabidopsis thaliana OX=3702 ...	[more]
Q9SSA8	1.5e-26	41.67	Ethylene-responsive transcription factor RAP2-12 OS=Arabidopsis thaliana OX=3702...	[more]
Q8H0T5	1.2e-25	49.63	Ethylene-responsive transcription factor ERF073 OS=Arabidopsis thaliana OX=3702 ...	[more]
Q9LUM4	1.2e-25	40.10	Ethylene-responsive transcription factor RAP2-2 OS=Arabidopsis thaliana OX=3702 ...	[more]

BLAST of Lag0031521 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3C7W9	1.6e-89	73.22	ethylene-responsive transcription factor RAP2-3-like OS=Cucumis melo OX=3656 GN=...	[more]
A0A6J1KF31	4.8e-89	75.00	ethylene-responsive transcription factor RAP2-3 OS=Cucurbita maxima OX=3661 GN=L...	[more]
A0A0A0KD18	3.4e-87	72.38	AP2/ERF domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G318160 PE=...	[more]
A0A6J1ER95	2.1e-84	71.67	ethylene-responsive transcription factor RAP2-3 OS=Cucurbita moschata OX=3662 GN...	[more]
A0A6J1CA63	7.9e-84	69.48	ethylene-responsive transcription factor RAP2-3-like OS=Momordica charantia OX=3...	[more]

BLAST of Lag0031521 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G16770.1	1.7e-41	46.46	ethylene-responsive element binding protein	[more]
AT1G53910.3	4.7e-28	42.13	related to AP2 12	[more]
AT2G47520.1	6.1e-28	54.96	Integrase-type DNA-binding superfamily protein	[more]
AT1G53910.1	1.0e-27	41.67	related to AP2 12	[more]
AT1G53910.2	1.0e-27	41.67	related to AP2 12	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 108..128
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 42..71
None	No IPR available	PANTHER	PTHR31190:SF210	EREBP TRANSCRIPTION FACTOR	coord: 1..137
IPR001471	AP2/ERF domain	PRINTS	PR00367	ETHRSPELEMNT	coord: 98..114 score: 73.69 coord: 76..87 score: 70.45
IPR001471	AP2/ERF domain	SMART	SM00380	rav1_2	coord: 75..138 e-value: 1.3E-38 score: 144.3
IPR001471	AP2/ERF domain	PFAM	PF00847	AP2	coord: 76..125 e-value: 3.9E-15 score: 55.8
IPR001471	AP2/ERF domain	PROSITE	PS51032	AP2_ERF	coord: 75..132 score: 23.828024
IPR001471	AP2/ERF domain	CDD	cd00018	AP2	coord: 76..132 e-value: 1.21301E-33 score: 113.906
IPR036955	AP2/ERF domain superfamily	GENE3D	3.30.730.10	AP2/ERF domain	coord: 75..133 e-value: 6.8E-27 score: 95.4
IPR044808	Ethylene-responsive transcription factor	PANTHER	PTHR31190	DNA-BINDING DOMAIN	coord: 1..137
IPR016177	DNA-binding domain superfamily	SUPERFAMILY	54171	DNA-binding domain	coord: 75..132

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0031521.1	Lag0031521.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009873	ethylene-activated signaling pathway
biological_process	GO:0006355	regulation of transcription, DNA-templated
cellular_component	GO:0005634	nucleus
molecular_function	GO:0003677	DNA binding
molecular_function	GO:0003700	DNA-binding transcription factor activity