Lag0031508 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0031508 vs. NCBI nr
Match: XP_042990668.1 (uncharacterized protein LOC122317666 [Carya illinoinensis])

HSP 1 Score: 227.6 bits (579), Expect = 2.8e-55
Identity = 207/781 (26.50%), Postives = 317/781 (40.59%), Query Frame = 0

Query: 17  ELCVIGKVFSSKWVNVEAFRSVMRAAWRVHGATHIESAVENLFAIQFHSRGEKGRVMSTG 76
           ELC++ K+F+ +  N EAFR+ M   W   G    +    N++ I+F    +K +V+   
Sbjct: 37  ELCLLAKIFNDRTANREAFRTTMSKIWNTEGWLTFKDLETNVYLIEFQLVSDKEKVLLGR 96

Query: 77  PWAFDRSLVVLKEPRSAGSVLDVEFNYCAFWVQIHNVPMRWQTREMARQLGDKIGTVEEV 136
           PW+FDR LV +KE     S+ +V F+   FW+Q+HN+P    ++EM   +G  IG V EV
Sbjct: 97  PWSFDRHLVCMKEFEGLLSLSEVRFDSEPFWIQVHNLPFAGMSKEMGILVGSVIGRVLEV 156

Query: 137 GGSQGGDWTGPILRIRDE---------------EGKEFRCPILYERLPDFCYDCGCLGNS 196
             +  G   G  LRI+ E                 K+      YERLP FC+ CG   + 
Sbjct: 157 ETNTEGYGWGGYLRIKAEVNVTKALVRGRFLKSGSKQSWLSFKYERLPMFCFKCGRFVHE 216

Query: 197 RRECGAVREGALEQQGEQYGDWLRAG------------AGL------GETGRRIQEGEVD 256
           +  C     GA     +QYG WLRA              GL      G T    QE   D
Sbjct: 217 QGSC--QERGADNHTFDQYGQWLRATHLHSKFQHNRRYGGLKEKEPSGSTWSGSQEEGDD 276

Query: 257 -----NAASRKPETPKPS--------------TEG---------QVTHDSVPKKAGKEIA 316
                  ++ KP +  P+              TEG          VT +    K  K   
Sbjct: 277 YSGFCGVSTSKPGSEPPNLVESTEALEVNLGHTEGNLPKEDQGTSVTDNPAHDKGHKTFP 336

Query: 317 N----------------------------ASGEGVEVQG----GGLEVMGKRGG------ 376
                                        AS   V +      G   +  + GG      
Sbjct: 337 THERPASWQERLSQDPTTLHPQVLFPHSLASSYDVVMLATEAPGPTSIRKENGGVPFTET 396

Query: 377 --------------GRNWKRRARDV---LSDVTN-------------------------- 436
                         G+ WKR+AR +   LSDVTN                          
Sbjct: 397 CFENLENSSNSPVKGKTWKRKARALPTPLSDVTNIIQQPSSYNNSKRSLRQRFSTRADGK 456

Query: 437 --RDVSLGPLMTPDQAG---SGETPQVLFLSETKVSASRMSSAKRLLGFDNCFCVDCHGR 496
             +  +  P  +  +       + P ++FL+ETK +  R+   K  LG++NCF V+  G+
Sbjct: 457 CKKQKTQAPFESVQELHLLVKAKQPHLVFLTETKCNNVRLDRIKLALGYENCFSVNSKGK 516

Query: 497 SGGLALMWVSSISSGPISFSKNHIDGWIL--WGGRRWRLTGFYGFPSADMHPQSWSLLSR 556
           SG LAL+W  S+    I+++  HI   I       +W+LTGFYG P++   P+SW LL  
Sbjct: 517 SGELALLWKDSVKVEVINYTTWHISALITSPIDNTQWQLTGFYGHPNSAKRPESWHLLRG 576

Query: 557 LRGCAETPWLIGGDFNTILCQDEKDGGRDKPL---------------------------- 614
           L+     PWL  GDFN I  Q EK G  ++P                             
Sbjct: 577 LKPTGNLPWLCLGDFNEITHQSEKVGAANRPYRQMVQFRNSLSFCKLYDLGFHGDKFTWS 636

BLAST of Lag0031508 vs. NCBI nr
Match: MCH80348.1 (hypothetical protein [Trifolium medium])

HSP 1 Score: 224.6 bits (571), Expect = 2.4e-54
Identity = 170/646 (26.32%), Postives = 273/646 (42.26%), Query Frame = 0

Query: 15  SIELCVIGKVFSSKWVNVEAFRSVMRAAWRVHGATHIESAVENLFAIQFHSRGEKGRVMS 74
           S    ++GK+++    N+ AF+  M  AWR      I+   +NL+  +F ++ E   V  
Sbjct: 30  SFSRTLVGKIWTESPYNIRAFKQTMTQAWRSRNPIEIQDLNKNLYLFKFATKREADLVYR 89

Query: 75  TGPWAFDRSLVVLKEPRSAGSVLDVEFNYCAFWVQIHNVPMRWQTREMARQLGDKIGTVE 134
            GPW+FDR+L++L          ++  ++ +FWV+++++P++ ++  +A++LG+ +GT E
Sbjct: 90  NGPWSFDRNLLILNIISGNEQPSELAMDHVSFWVRVYDLPLKLRSEGIAKKLGNTVGTFE 149

Query: 135 EVGGSQGGDWTGPILRIR---------------DEEGKEFRCPILYERLPDFCYDCGCLG 194
           E+   +  +  G  LR+R               + +GKE      YERLP+FC+ CG +G
Sbjct: 150 EMDFKE-INRMGKFLRMRVSVDLKKPLKRGTKLNFQGKEIWVDYKYERLPNFCFACGRIG 209

Query: 195 NSRREC------GAVREGALEQQGEQYGDWLRA--------------------------- 254
           +  R+C        V+   LE++ + +G WLRA                           
Sbjct: 210 HQMRDCEDIEDHDEVQYSELEEKQQAFGPWLRASPLPKMSYEVKKESSSSNCSKSLFPST 269

Query: 255 ----GAGLGETGRRIQEGEVDNAASRKPETPKPSTEGQVTHDSVPKKAGKEIANASGEGV 314
               G   G T + + E       S    + +  T+G ++ D+    + ++      E V
Sbjct: 270 SNSKGQNSG-TAKEMDEEVEQQRVSDDQHSQQKITKGNMSKDAEGDTSKQQNVQKEVEVV 329

Query: 315 EVQGGGLEVMG-----------KRGGGRNWKRR-----------------ARDVLSDVTN 374
               G + +             K G GR W R+                  +  L DV  
Sbjct: 330 AESLGTVTISAQTKIGEGTQTVKEGKGRRWVRQKVTKPKPQKNAKTVGKIGKRSLVDVMV 389

Query: 375 RDVSL-----------GPLMTPDQAGSG---------------ETPQVLFLSETKVSASR 434
            D ++           G ++  D   S                E PQ++FL ET++  + 
Sbjct: 390 TDGTMEAMKGGEKKIRGDVVMKDCVASTGSPRAVRALLRLNRVENPQLVFLMETRLKVTE 449

Query: 435 MSSAKRLLGFDNCFCVDCHG----RSGGLALMWVSSISSGPISFSKNHIDGWI--LWGGR 494
           + + +  LGF NC  VDC+G    R+GGLALMW+  +S    SFS NHI G       G 
Sbjct: 450 IENIRSKLGFKNCLAVDCNGFGRERAGGLALMWMEHLSVNISSFSLNHIHGRCEDEESGE 509

Query: 495 RWRLTGFYGFPSADMHPQSWSLLSRLRGCAETPWLIGGDFNTILCQDEKDGGR------- 519
            W LTG YG+P      ++W L+  L    +  WL  GDFN IL   EK GG        
Sbjct: 510 SWGLTGIYGYPEEHNKRKTWHLIRSLARQNQGRWLCCGDFNDILDSHEKQGGNVRSQTQF 569

BLAST of Lag0031508 vs. NCBI nr
Match: KAF5446505.1 (hypothetical protein F2P56_032128 [Juglans regia])

HSP 1 Score: 222.2 bits (565), Expect = 1.2e-53
Identity = 150/524 (28.63%), Postives = 234/524 (44.66%), Query Frame = 0

Query: 17  ELCVIGKVFSSKWVNVEAFRSVMRAAWRVHGATHIESAVENLFAIQFHSRGEKGRVMSTG 76
           EL ++GK+++ + +N     S M   WR+      +   +N++ + F +  +K R+M   
Sbjct: 40  ELSLVGKIWTGRSINQAVVESTMAKVWRISKRAKFKGVGDNVYVMVFVNHADKQRIMEGR 99

Query: 77  PWAFDRSLVVLKEPRSAGSVLDVEFNYCAFWVQIHNVPMRWQTREMARQLGDKIGTVEEV 136
           PW FD  L V+K+         + F    FW+Q+H +P+    +     +G  +G V+EV
Sbjct: 100 PWLFDNQLFVIKQYDGLTPPSKINFKSECFWIQMHELPLVCMNKVRGEIIGGTVGRVKEV 159

Query: 137 GGSQGGDWTGPILRIRDE---------------EGKEFRCPILYERLPDFCYDCGCLGNS 196
              + G   G  LR+  E                GK F     YE+LP  C+ CGC+ + 
Sbjct: 160 DAQEDGCGWGEYLRVLIEVDLEKPLARGRSITVRGKNFWTQFKYEKLPHICFRCGCILHG 219

Query: 197 RRECGAVREGALEQQGEQYGDWLRAGAGLGETGRRIQEGEVDNAASRKPETPKPSTEGQV 256
            + C    +G  E++  QYG W+RAG      G++ +EG   +   +  E      EG+ 
Sbjct: 220 DQGC----KGNGEREA-QYGVWMRAGF----KGKK-REGGFHSFVFQGKEMENTDGEGRE 279

Query: 257 THDSVPKKAGKEIANASGEGVEVQGGGLEVMGKRGGGRNWKRRARDVLSDV--TNRDVSL 316
               +  +          E    +G   E + + G G   +R   +++ +V    +  ++
Sbjct: 280 NSLKIITRQEIREEQRDPEITVQEGENSEKVVESGRGGEDEREKEEMVKEVEGNKKGENI 339

Query: 317 GPL-----MTPDQAGSGETPQVLFLSETKVSASRMSSAKRLLGFDNCFCVDCHGRSGGLA 376
           G +     +  ++ G  + P V+FL ETK+ + +    KR L  D CF V+  GR GGL 
Sbjct: 340 GKVKVLEKVDSERGGEDKRPNVIFLIETKMRSGKSERIKRRLNCDGCFVVEPVGRKGGLM 399

Query: 377 LMWVSSISSGPISFSKNHIDGWIL---WGGRRWRLTGFYGFPSADMHPQSWSLLSRLRGC 436
           L+W  S+    +++S+ HI  WI        RW LTGFYG P   M  ++W LL  L+  
Sbjct: 400 LLWDYSMEVEILNYSQRHISAWITSEEGKNERWLLTGFYGNPETSMREEAWQLLGFLKPL 459

Query: 437 AETPWLIGGDFNTILCQDEKDGGRDKP----------LRPGG------------------ 486
           A   W + GDFN IL  DEK GGR +P          L  GG                  
Sbjct: 460 ASCGWCVIGDFNEILTHDEKWGGRSRPEKQMSRFREVLEEGGLFDLGWRGDKYTWSNKHE 519

BLAST of Lag0031508 vs. NCBI nr
Match: XP_035544642.1 (uncharacterized protein LOC109020982 [Juglans regia])

HSP 1 Score: 220.3 bits (560), Expect = 4.5e-53
Identity = 201/771 (26.07%), Postives = 300/771 (38.91%), Query Frame = 0

Query: 1   MVRPGEGTPVLSMGSIELCVIGKVFSSKWVNVEAFRSVMRAAWRVHGATHIESAVENLFA 60
           MV P + T  ++M   + C++G + + K +N EAFR+ M   WR  G        EN F 
Sbjct: 22  MVLPSD-TMEMAMHQGKFCLLGMIIAEKPINKEAFRNTMIKVWRSEGWVQFTEVGENRFL 81

Query: 61  IQFHSRGEKGRVMSTGPWAFDRSLVVLKEPRSAGSVLDVEFNYCAFWVQIHNVPMRWQTR 120
           I+F+   ++ RV+   PW+FDR L+ L        V D++F    FW+Q+HN+P+   T 
Sbjct: 82  IEFNKDEDRQRVIKGRPWSFDRWLLCLHAFEGNMPVNDIQFTREEFWLQVHNMPLGTMTE 141

Query: 121 EMARQLGDKIGTVEEVGGSQGGDWTGPILRIRDE---------------EGKEFRCPILY 180
           E+ +Q+G  +G V +V     G   G  +RIR E               +G++      Y
Sbjct: 142 EVGKQIGKNVGKVLKVHSDDRGIGWGKFMRIRVEMYITKALMRGMFLTFDGRKTWVQFKY 201

Query: 181 ERLPDFCYDCGCLGNSRRECGAVREGALEQQGEQYGDWLRAGA----------------- 240
           ERLP FC  CG + ++ + C +  + + +    QYG WLRA A                 
Sbjct: 202 ERLPTFCLKCGVIRHNGKSCQSSPKASPQ---NQYGVWLRAPAAKEGDINQKRYGHDSSQ 261

Query: 241 -------------------GLGE------------TGRRIQEG----------------- 300
                               LG+            T   IQE                  
Sbjct: 262 QTSGADHSWRNDGKVGDEVNLGKAVSKEGFKDPINTSDPIQENCVFLQPTKETSADLVES 321

Query: 301 ------------------EVDNAASRKPETPKPSTEGQVTHDSVPKKAGKEIANASGEGV 360
                             E D       E P P+ E Q      P K G    ++S +  
Sbjct: 322 LMATDTQLPKVNVTLMDLEGDTGLPTLKEDPTPTREVQ---QKSPPKPGPSKRDSSLKNY 381

Query: 361 EVQGG-----GLEVMGKRGGGRNWKRRARDVLSDVTNRDVSLGPLMTPDQAGSGETPQVL 420
           E          L     R   R W+   R+ L      +     ++    +   + P  +
Sbjct: 382 EPNSDLRPRLILLTNSSRIKPRFWQLPKREPLG---KEEPERSAMLICFLSLKSKLPSFV 441

Query: 421 FLSETKVSASRMSSAKRLLGFDNCFCVDCHGRSGGLALMWVSSISSGPISFSKNHIDGWI 480
           FL ETK    ++   KRL+ FDN F +DC G SGGLA +W + + +   S+S+NHI   +
Sbjct: 442 FLMETKCKRHKVECVKRLIKFDNSFVIDCKGFSGGLAFLWKNDVEAEVHSYSQNHISLMV 501

Query: 481 LWGGRR--WRLTGFYGFPSADMHPQSWSLLSRLRGCAETPWLIGGDFNTILCQDEKDGGR 540
                R  W LTGFYG P       SW LL  +       WL  GDFN IL   +K GG 
Sbjct: 502 KGAKEREDWLLTGFYGSPVTARRQSSWKLLQAINPVHPLGWLCIGDFNEILFIGDKSGGA 561

Query: 541 DKPL------------------------------RPGGETIYERLDRAFGNTPWTDLYPN 600
            +                                R G     E LDRAFGN+ W DL+P+
Sbjct: 562 VRAFNQIESFRDTVELCGLSDLGFVGNKFTWSNGRHGASFTKEMLDRAFGNSSWMDLFPS 621

Query: 601 YVVNHLDYSRSDHRPV----ELILTPSPQC---------WSQSGQRIARFEETWLRQPDL 609
           + V  L    SDH P+    E  +T S +C         WS +G+     EE W +    
Sbjct: 622 HKVYTLPALSSDHCPIFVTMEKQVTDSLKCDKPFRFEAGWSLNGECHNILEEVWTKPRMA 681

BLAST of Lag0031508 vs. NCBI nr
Match: XP_022149484.1 (uncharacterized protein LOC111017902 [Momordica charantia])

HSP 1 Score: 216.1 bits (549), Expect = 8.5e-52
Identity = 96/216 (44.44%), Postives = 135/216 (62.50%), Query Frame = 0

Query: 7   GTPVLSMGSIELCVIGKVFSSKWVNVEAFRSVMRAAWRVHGATHIESAVENLFAIQFHSR 66
           G P+L+  +++LCV+ K+ +SK ++ EA RSVM++ WRVH +T  E    N++ I F S 
Sbjct: 26  GKPILTADNVKLCVVAKLHTSKRISAEAIRSVMKSVWRVHNSTRFEPLGMNIYVILFKSL 85

Query: 67  GEKGRVMSTGPWAFDRSLVVLKEPRSAGSVLDVEFNYCAFWVQIHNVPMRWQTREMARQL 126
            EK RV+S+GPW F++SL+VL  P +    LD+ FN+CAFW+QIHN+P    + EMA  L
Sbjct: 86  SEKSRVLSSGPWTFNKSLLVLTSPTATNQPLDMNFNFCAFWIQIHNIPFECISTEMANIL 145

Query: 127 GDKIGTVEEVGGSQGGDWTGPILRIR----------------DEEGKEFRCPILYERLPD 186
           G K+G VEE+ G     W GP +R+R                + +GK+  CP+ YE+LPD
Sbjct: 146 GAKLGDVEEIEGDGADGWAGPFIRVRVKIDVSKPLRRGIKLKNSDGKDIWCPLRYEKLPD 205

Query: 187 FCYDCGCLGNSRRECGAVREGALEQQGEQYGDWLRA 207
           FCY+CG +G+S REC    +       EQYGDWLRA
Sbjct: 206 FCYECGKIGHSGRECEQRSKVVTTNSPEQYGDWLRA 241

BLAST of Lag0031508 vs. ExPASy TrEMBL
Match: A0A2N9GJ35 (Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS27322 PE=3 SV=1)

HSP 1 Score: 249.6 bits (636), Expect = 3.4e-62
Identity = 174/577 (30.16%), Postives = 265/577 (45.93%), Query Frame = 0

Query: 20  VIGKVFSSKWVNVEAFRSVMRAAWRVHGATHIESAVENLFAIQFHSRGEKGRVMSTGPWA 79
           ++ K+ ++K  + EAF+  +RA W   G   I S   NLF   F  R +  R+    PW 
Sbjct: 40  ILFKLLTTKPFHSEAFKGSIRALWSGLGGVTIRSIEGNLFMAVFTRRDDMERIFVRSPWT 99

Query: 80  FDRSLVVLKEPRSAGSVLDVEFNYCAFWVQIHNVPMRWQTREMARQLGDKIGTVEEVGGS 139
           FD+ L+ +          +V F++ AFW+++ N+P++   RE+   +G +IG + EV   
Sbjct: 100 FDKKLIPIVRFEGDLQPTEVRFSHTAFWIRVFNLPIKSMIREVGEDIGQEIGRLLEVDVP 159

Query: 140 QGGDWTGPILRIR-------------------DEEGKEFRCPILYERLPDFCYDCGCLGN 199
           + G   G  LRIR                    + G  F     YE LP FCY CG LG+
Sbjct: 160 ENGFGWGEYLRIRVEIDIAQPLLRGCILQSDESDGGGLFWVDFKYEHLPIFCYRCGRLGH 219

Query: 200 SRRECGAVREGALEQ--QGEQYGDWLRAGAGLGETGRRIQEGEVDNAASRKPETPKPSTE 259
              EC   R G + +   GE++G WLRA A      RR +EG             +P  E
Sbjct: 220 GSHECVVGRGGRISEGVSGEKWGAWLRALAARPAQPRRSREGVF-----------QPDEE 279

Query: 260 GQVTHDSVPKKAGKEIANASGEGVEVQGGG------------------LEVMGKRGGGRN 319
           G+    ++P    +E A  +     V GGG                  LE+  +R    +
Sbjct: 280 GE---SNMP--FDREAATENDPSPPVSGGGCKLWDGHWLHELLEEIMQLEMHVERPACSS 339

Query: 320 WKRRARDVLSDVTNRDVSLGPLMTPDQAGS---GETPQVLFLSETKVSASRMSSAKRLLG 379
            K      +  ++     LG   T ++  +    E P ++FL ET+++   +   +  LG
Sbjct: 340 GKDAPPVTMRALSLNCRGLGNPQTVNELHNLVKKEGPNIVFLMETRLNVRNLEWLRVRLG 399

Query: 380 FDNCFCVDCHGRSGGLALMWVSSISSGPISFSKNHIDGWILWG-GRRWRLTGFYGFPSAD 439
              C  V+ HG+ GGLAL+W SS+     S+S++HIDG ++   G RWRLTGFYG+P A 
Sbjct: 400 MKGCLGVERHGQGGGLALLWDSSVMINIQSYSEHHIDGEVVQNDGLRWRLTGFYGYPEAH 459

Query: 440 MHPQSWSLLSRLRGCAETPWLIGGDFNTILCQDEKDGGRDKPL----------------- 499
           +  +SWSLL  LR  ++ PW+I GDFN I   +EK G  D+                   
Sbjct: 460 LRHRSWSLLRHLRSISDVPWMIFGDFNEITRLEEKAGREDRNANQMAAFREALLDCSLQD 519

Query: 500 -------------RPGGETIYERLDRAFGNTPWTDLYPNYVVNHLDYSRSDHRPVELIL- 520
                        R  G+ +  RLDR   +  W  L+P+  +NHL  + SDH  V L+L 
Sbjct: 520 MGFTGTEFTWSNNRENGDLVRVRLDRGVADAAWVQLFPHASINHLIVASSDH--VGLLLD 579

BLAST of Lag0031508 vs. ExPASy TrEMBL
Match: A0A2N9FI47 (CCHC-type domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS18368 PE=4 SV=1)

HSP 1 Score: 240.4 bits (612), Expect = 2.0e-59
Identity = 188/712 (26.40%), Postives = 295/712 (41.43%), Query Frame = 0

Query: 23  KVFSSKWVNVEAFRSVMRAAWRVHGATHIESAVENLFAIQFHSRGEKGRVMSTGPWAFDR 82
           K+ +S+  N EAF++ + A W VHG   I    +NLF   F +     R+ +  PW FD+
Sbjct: 43  KLQTSRQFNGEAFKNSVLAIWTVHGDLTISEFDDNLFLAAFSTEAALNRIFALSPWTFDK 102

Query: 83  SLVVLKEPRSAGSVLDVEFNYCAFWVQIHNVPMRWQTREMARQLGDKIGTVEEVG-GSQG 142
            L++L           V+F +  FW+++ N+P++  TRE+   +G ++G + +V   ++ 
Sbjct: 103 KLILLARLEGDLQPSAVKFTHTIFWIKVVNLPIKCMTREVGEDIGMQVGKLLDVDVPNEN 162

Query: 143 GDWTGPILRIR----------------DEEGKEFRCPILYERLPDFCYDCGCLGNSRREC 202
           G   G  LRIR                 E+ K       YE LP FCY CG LG+S  +C
Sbjct: 163 GIAWGRFLRIRVEVELAKPLMRGCIIQVEDDKPVWVDFRYEHLPIFCYKCGFLGHSSSDC 222

Query: 203 GAVREGALEQ--QGEQYGDWLRA---------------GAGLG--ETGRRIQEGEVDNAA 262
            A R  A       +QYG WLRA               G GLG        +E   D   
Sbjct: 223 TANRSSARVSVFDRDQYGSWLRALPAQHHQLARRKSEVGDGLGNQSNSNSHEENGSDGGG 282

Query: 263 SRKPETPKPSTEGQVTHDSVPKKAGKEIANAS--GEGVEVQGGGLEV-------MGKRGG 322
             KPET +         D+   ++  E   A    E ++ +   L V       +     
Sbjct: 283 GGKPETLREDRIEVTLVDNQALRSRSEHVRAPDLAESMDTEKEVLHVPSFMDTQLAPPNT 342

Query: 323 GRNWKRRARDVLSDVTNRDVSLGPLMTPDQAGSGETPQVLFLSETKVSASRMSSAKRLLG 382
            + +    R V S  T R++ +            E P ++FL ET +    +   +  LG
Sbjct: 343 MKTFSLNCRGVGSLETVRELHM--------LVKKEDPNIVFLMETHLEVRCLEFVRVRLG 402

Query: 383 FDNCFCVDCHGRSGGLALMWVSSISSGPISFSKNHIDGWILWGGR-RWRLTGFYGFPSAD 442
              CF VD HG  GGLAL+W SS+S    S+S  HID  ++     +WR+TGFYG P   
Sbjct: 403 MCGCFGVDRHGFGGGLALLWRSSVSVHIQSYSIFHIDADVVMEDELKWRITGFYGHPERG 462

Query: 443 MHPQSWSLLSRLRGCAETPWLIGGDFNTILCQDEKDGGRDKPL----------------- 502
           +   SW+LL +L      PWL+ GDFN ++  +E+ G  ++ L                 
Sbjct: 463 LRVSSWALLRQLHDARSLPWLVMGDFNEVMSLEEQWGRINRSLPQMAAFRDALLDCSLQD 522

Query: 503 -------------RPGGETIYERLDRAFGNTPWTDLYPNYVVNHLDYSRSDHRPVELILT 562
                        R  G  +  +LDR   N  W  L+P+Y V+H+ ++ SDH  + +++ 
Sbjct: 523 LGYQGPDFSWSNRREDGALVRAQLDRGVANNAWLSLFPSYQVHHVVFAASDHMGLLILMN 582

Query: 563 PSPQCWSQSGQRIARFEETWLRQ------------------------------------- 604
           P     S + +++ RF+ +W+R+                                     
Sbjct: 583 PPQASPSINRKKLFRFDHSWVRELGCEETIKMAWSCPVSGTPMFRVAQKIKNCRMQLLQW 642

BLAST of Lag0031508 vs. ExPASy TrEMBL
Match: A0A2N9I611 (Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS47331 PE=4 SV=1)

HSP 1 Score: 231.9 bits (590), Expect = 7.3e-57
Identity = 169/539 (31.35%), Postives = 247/539 (45.83%), Query Frame = 0

Query: 3   RPGEGTPVLSMGSIELCVIGKVFSSKWVNVEAFRSVMRAAWRVHGATHIESAVENLFAIQ 62
           +  E T V+  G   L  IGK+  SK  N EA   VM   W+   +  ++   ENLF  +
Sbjct: 25  KESEKTLVIQKGKKSL--IGKLAGSKTANREALCRVMNMLWKPRKSLKVQEIGENLFIFE 84

Query: 63  FHSRGEKGRVMSTGPWAFDRSLVVLKEPRSAGSVLDVEFNYCAFWVQIHNVPMRWQTREM 122
           F    +K RV+   PW FDR +++L E  S     +  F     WVQ+ ++P+   T E+
Sbjct: 85  FTEERDKARVLGGCPWLFDRHMLLLHEFDSLTPPREFIFKSSPLWVQVFDLPLYHMTEEV 144

Query: 123 ARQLGDKIGTVEEVGGSQGGDWTGPILRIRDEEGKEFRCPILYERLPDFCYDCGCLGNSR 182
              +G+ +G   E   ++ G   G  LR              YERLP FC+ CG +G+  
Sbjct: 145 GELIGNAMGGHIETEVTEDGVGWGKYLR--------------YERLPLFCHACGIIGHGE 204

Query: 183 RECGAVREGALEQQGE----QYGDWLRAGAGLGETGRRIQEGEVDNAASRKPETPKPSTE 242
           +EC  V     ++QG     QYG WLRA   +  +G+        N  + + E  +   E
Sbjct: 205 KEC--VTRRNQQEQGRVGTLQYGAWLRADFHIKRSGQSFG----PNPTAAEEEEDEVVAE 264

Query: 243 GQVTHDSVPKKAGKEIAN----ASGEGVEVQGGGLEVMGKRGGGRNWKRRARDVLSDVTN 302
              T + + K+AG E +N      GE    Q   L   G RG G     RA   L D+  
Sbjct: 265 ---TKEPMQKEAG-ECSNLRRVGKGEEHREQERCLTEGGSRGLG---NPRAVRTLRDLQK 324

Query: 303 RDVSLGPLMTPDQAGSGETPQVLFLSETKVSASRMSSAKRLLGFDNCFCVDCHGRSGGLA 362
                            + P+VLFLSETK++   M   +  L +D+ F V   GRSGGLA
Sbjct: 325 M----------------KAPKVLFLSETKLNVEEMEWIRVGLKYDSAFVVPSKGRSGGLA 384

Query: 363 LMWVSSISSGPISFSKNHIDGWILWGGRRWRLTGFYGFPSADMHPQSWSLLSRLRGCAET 422
           L+W   +     S+++ HID  I      WR TGFYG P       SW+LL  L+  +  
Sbjct: 385 LLWDEDVDLSITSYTRFHIDAQIKSQEGLWRFTGFYGHPETAKRKGSWALLKYLKEISPL 444

Query: 423 PWLIGGDFNTILCQDEKDG-----GRDKPLRPGGETIYERLDRAFGNTPWTDLYPNYVVN 482
           PW+  GDFN IL  DEK G      RD     GG     RLDR FGN  W +++ N ++ 
Sbjct: 445 PWVCMGDFNEILSGDEKAGVTWNNSRDVEGFSGG-----RLDRGFGNMLWKEMFKNAMIV 504

Query: 483 HLDYSRSDHRPVELILTPSPQCWSQSGQRIARFEETWLRQPDLRQLVQSAIADLSTSDS 529
           ++  S SDH  + L+L  + Q   +   R+ RFE+ W +  +  +++     D  + ++
Sbjct: 505 NIPVSYSDHMAISLVLLCNDQL-RKRAPRLFRFEQAWTKHEECEKVIVDVWRDTESPET 512

BLAST of Lag0031508 vs. ExPASy TrEMBL
Match: A0A2N9FB16 (CCHC-type domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS12200 PE=4 SV=1)

HSP 1 Score: 226.1 bits (575), Expect = 4.0e-55
Identity = 164/548 (29.93%), Postives = 248/548 (45.26%), Query Frame = 0

Query: 20  VIGKVFSSKWVNVEAFRSVMRAAWRVHGATHIESAVENLFAIQFHSRGEKGRVMSTGPWA 79
           ++ K+ ++K  + EAF+  +RA W   G   I S   NLF   F  R +  R+    PW 
Sbjct: 40  ILFKLLTTKPFHSEAFKGSIRALWSGLGGVTIRSIEGNLFMAVFTRRDDMERIFVRSPWT 99

Query: 80  FDRSLVVLKEPRSAGSVLDVEFNYCAFWVQIHNVPMRWQTREMARQLGDKIGTVEEVGGS 139
           FD+ L+ +          +V F++ AFW+++ N+P++   RE+   +G +IG + EV   
Sbjct: 100 FDKKLIPIVRFEGDLQPTEVRFSHTAFWIRVFNLPIKSMIREVGEDIGQEIGRLLEVDVP 159

Query: 140 QGGDWTGPILRIR-------------------DEEGKEFRCPILYERLPDFCYDCGCLGN 199
           + G   G  LRIR                    + G  F     YE LP FCY CG LG+
Sbjct: 160 ENGFGWGEYLRIRVEIDITQPLLRGCILQSDESDGGGLFWVDFKYEHLPIFCYRCGRLGH 219

Query: 200 SRRECGAVREGALEQ--QGEQYGDWLRAGAGLGETGRRIQEG--------------EVDN 259
              EC   R G + +   GE++G WLRA A      RR +EG              + + 
Sbjct: 220 GSHECVVGRGGRISEGVSGEKWGAWLRALAARPAQPRRSREGVFQPDEEGESNMPFDREA 279

Query: 260 AASRKPETPKP---STEGQVTHDSVPKKAGKEIANASGEGVEVQGGGLEVMGKRGGGRNW 319
           A    P  P P   +  G  T  +       E+       +  +  G     K  G  + 
Sbjct: 280 ATENDPSPPVPVAVANSGTGTGSTFFNADNAEVVEPVMCKIRSEDQG---HAKISGEEDH 339

Query: 320 KRRARDVLS-DVTNRDVSLG--PLMTPDQAG------SGETPQVLFLSETKVSASRMSSA 379
               ++V S  VT R +SL    L  P            E P ++FL ET+++   +   
Sbjct: 340 VPNVKEVHSPPVTMRALSLNCRGLGNPQTVNELHNLVKKEGPNIVFLMETRLNVRNLEWL 399

Query: 380 KRLLGFDNCFCVDCHGRSGGLALMWVSSISSGPISFSKNHIDGWILWG-GRRWRLTGFYG 439
           +  LG   C  V+ HG+ GGLAL+W SS+     S+S++HIDG ++   G RWRLTGFYG
Sbjct: 400 RVRLGMKGCLGVERHGQGGGLALLWDSSVMINIQSYSEHHIDGEVVQNDGLRWRLTGFYG 459

Query: 440 FPSADMHPQSWSLLSRLRGCAETPWLIGGDFNTILCQDEKDGGRDKPLRPGGETIYERLD 499
           +P A +  +SWSLL  LR  ++ PW+I GDFN I   +EK G  D+            LD
Sbjct: 460 YPEAHLRHRSWSLLRHLRSISDVPWMIFGDFNEITRLEEKVGREDRNANQMAAFREALLD 519

Query: 500 RAFGNTPWTDLYPNYVVNHLDYSRSDHRPVELILTPSPQCWSQSGQRIARFEETWLRQPD 520
            +  +  +TD     + +  D  R +H P             Q  +R+ RFE++WL++  
Sbjct: 520 CSLQDMGFTDHVGLLLDSRTDQPR-NHVP-------------QRKRRMFRFEKSWLKESG 570

BLAST of Lag0031508 vs. ExPASy TrEMBL
Match: A0A392M033 (CCHC-type domain-containing protein (Fragment) OS=Trifolium medium OX=97028 GN=A2U01_0001115 PE=4 SV=1)

HSP 1 Score: 224.6 bits (571), Expect = 1.2e-54
Identity = 170/646 (26.32%), Postives = 273/646 (42.26%), Query Frame = 0

Query: 15  SIELCVIGKVFSSKWVNVEAFRSVMRAAWRVHGATHIESAVENLFAIQFHSRGEKGRVMS 74
           S    ++GK+++    N+ AF+  M  AWR      I+   +NL+  +F ++ E   V  
Sbjct: 30  SFSRTLVGKIWTESPYNIRAFKQTMTQAWRSRNPIEIQDLNKNLYLFKFATKREADLVYR 89

Query: 75  TGPWAFDRSLVVLKEPRSAGSVLDVEFNYCAFWVQIHNVPMRWQTREMARQLGDKIGTVE 134
            GPW+FDR+L++L          ++  ++ +FWV+++++P++ ++  +A++LG+ +GT E
Sbjct: 90  NGPWSFDRNLLILNIISGNEQPSELAMDHVSFWVRVYDLPLKLRSEGIAKKLGNTVGTFE 149

Query: 135 EVGGSQGGDWTGPILRIR---------------DEEGKEFRCPILYERLPDFCYDCGCLG 194
           E+   +  +  G  LR+R               + +GKE      YERLP+FC+ CG +G
Sbjct: 150 EMDFKE-INRMGKFLRMRVSVDLKKPLKRGTKLNFQGKEIWVDYKYERLPNFCFACGRIG 209

Query: 195 NSRREC------GAVREGALEQQGEQYGDWLRA--------------------------- 254
           +  R+C        V+   LE++ + +G WLRA                           
Sbjct: 210 HQMRDCEDIEDHDEVQYSELEEKQQAFGPWLRASPLPKMSYEVKKESSSSNCSKSLFPST 269

Query: 255 ----GAGLGETGRRIQEGEVDNAASRKPETPKPSTEGQVTHDSVPKKAGKEIANASGEGV 314
               G   G T + + E       S    + +  T+G ++ D+    + ++      E V
Sbjct: 270 SNSKGQNSG-TAKEMDEEVEQQRVSDDQHSQQKITKGNMSKDAEGDTSKQQNVQKEVEVV 329

Query: 315 EVQGGGLEVMG-----------KRGGGRNWKRR-----------------ARDVLSDVTN 374
               G + +             K G GR W R+                  +  L DV  
Sbjct: 330 AESLGTVTISAQTKIGEGTQTVKEGKGRRWVRQKVTKPKPQKNAKTVGKIGKRSLVDVMV 389

Query: 375 RDVSL-----------GPLMTPDQAGSG---------------ETPQVLFLSETKVSASR 434
            D ++           G ++  D   S                E PQ++FL ET++  + 
Sbjct: 390 TDGTMEAMKGGEKKIRGDVVMKDCVASTGSPRAVRALLRLNRVENPQLVFLMETRLKVTE 449

Query: 435 MSSAKRLLGFDNCFCVDCHG----RSGGLALMWVSSISSGPISFSKNHIDGWI--LWGGR 494
           + + +  LGF NC  VDC+G    R+GGLALMW+  +S    SFS NHI G       G 
Sbjct: 450 IENIRSKLGFKNCLAVDCNGFGRERAGGLALMWMEHLSVNISSFSLNHIHGRCEDEESGE 509

Query: 495 RWRLTGFYGFPSADMHPQSWSLLSRLRGCAETPWLIGGDFNTILCQDEKDGGR------- 519
            W LTG YG+P      ++W L+  L    +  WL  GDFN IL   EK GG        
Sbjct: 510 SWGLTGIYGYPEEHNKRKTWHLIRSLARQNQGRWLCCGDFNDILDSHEKQGGNVRSQTQF 569

BLAST of Lag0031508 vs. TAIR 10
Match: AT3G42140.1 (zinc ion binding;nucleic acid binding )

HSP 1 Score: 61.2 bits (147), Expect = 3.3e-09
Identity = 34/123 (27.64%), Postives = 55/123 (44.72%), Query Frame = 0

Query: 63  FHSRGEKGRVMSTGPWAFDRSLVVLKEPRSAGSVLDVEFNYCAFWVQIHNVPMRWQTREM 122
           F S      ++  GPW+F+  + V++      S  D EF    FW+QI  +P+R+ T  +
Sbjct: 66  FQSEESMFSILRRGPWSFNDWMCVIQRWTKLHS--DAEFKRIPFWIQIRGIPLRFLTARI 125

Query: 123 ARQLGDKIGTVEEVGGSQGGDWTGPILRIRDEEGKEFRCPILYERLPDFCYDCGCLGNSR 182
              +G+++G   E                RD    +F+    YE+L +FC  CG L +  
Sbjct: 126 ITSIGERMGLFLETNLG------------RDVSVLKFQ----YEKLKNFCTTCGMLSHDA 170

Query: 183 REC 186
            EC
Sbjct: 186 SEC 170