Lag0031495 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0031495
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	Pectinesterase
Location	chr11: 9235100 .. 9236913 (-)
RNA-Seq Expression	Lag0031495
Synteny	Lag0031495

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCGAACGCCGAAGGTGACAAAAAGAAGAAGAATATCGCCATAGTAAGCGTCTCTTCTCTGATCCTCGTTGCAATGGTGGTCGCCGTTACTGTCGGCGTCAACAGCTCACCCTACGGTGACGATCACGGTGAGGGTCATGGTGACGGTCACGGTGACGGAGGTGCCGAGATTAAGACATCGACGAAAGCAATTCAATCCATTTGTCGACCAACGGATTACAAAGAAACCTGCGAGCAGAGCCTGATGGCCGAAGCCAGTAACACGACGGACCCGAAAGAGCTCGCGAAAGCCGGCTTCAAAGTCGCCGTCGAATATATAAACACGGCGATTAGAAATTCCACCACACTCAAAGAGCTGGCCAAGGAACCAAGAACCAACCAAGCCCTTCAGAACTGCCGCGAGCTTCTGGAATACGCCATCGACGATTTAACCCAATCTTTCGAAAGAATCGGCACCTTCGAAATGTCGAAACTCGACCAGTTCGTCAACGATTTGAAGATCTGGCTTAGTGGGGCCCTCACGTACGAGCAGACTTGTTTGGACGGATTCGAAAACACGACCGGTGATGCCGGTCAGAAAATGCAGCAGTTTTTGAAGTCCGCTCAGGAAATGACGAGTAACGGTCTTGCGATCGTAACTGAATTGTCCACTATTATGGGATCACTTAATCAAATGCCGGGAGCGACGCCGGGAGCGACGGGACGACGGCTGTTGGACGAGGAAATGCCGTCGTGGGTGAGCGAAGGAAAGAGGCGGCTGATGCAGGCGGGACCGGGGGGGCTGAAGCCGGACGTGGTGGTGGCTCAAGATGGAAGTGGAAAATACAAGACGATCAGCGCGGCTTTGGTTGATGTGCCGAAGAAGAGTAATAAGACATTTGTGATTTATGTGAAGGCTGGAATTTATAAGGAAATCGTTGTTGTTCCTAAGGATGTGACTCATCTTACGATGTACGGCGATGGCCCAACCAAGACTGTCGTCACTGGTAGTCTCAACTTTATCGATGGCATTCAAACATTCAAGACCGCAACTTTCTGTAAGTCCAATAATATATAAATATATTTTTACTCTTTTATATTGCTAAAGTAGTCTAATGAATTGGATTTGATATTTAAACAGCTACGATCGGAACTGATTTCTTCGCTAAAGACATGGGATTCGTGAACAGTGCTGGAGCTGCAAAGCACCAAGCCGTGGCGTTACGAGTCCAATCCGATAGAGCGATCTTTTACAACTGCCAAATGGATGGGTATCAAGATACTCTCTACGCCCACGCCCATCGCCAGTTCTACCGTGACTGTTCGATCAGTGGCACCATCGACTTCGTCTTCGGCAACGCGGCCGCCGTCTTCCAGAACTGCAAACTAATCGTCCGAAAGCCAATGGATAACCAACAATGCATCGTCACGGCTCAAGGCCGGCTCAACCGCCGCGAACCGACCGCCCTAGTGTTCCAAGGGTGCCACTTCATGGGCGATCCGGCTTACTTGCCATTCAAGGCCGTCAACAAGGCTTACTTGGGGCGACCCTGGAAGGAATATTCACGAACCCTCATTATCGGGTCTCAAATCGACGATTTGATCCAGCCCGAGGGATGGTTGCCTTGGGGTGGCAATTTTGGGCTGAACACGCTGTTCTACGCGGAGGTTCAGAACAAGGGACCAGGGGCCGATCTTAGCAAGAGGGTGAAGTGGCGAGGAATTAAAACTATTACGCCAGATCACGCCGCTAAATTCACTCCCAGGGTATTTATCGACGGCGATGCTTGGATTAAGCCCAAGGGAATTCCGTATGCTTCTGGAATGATGTAA

mRNA sequence

ATGTCGAACGCCGAAGGTGACAAAAAGAAGAAGAATATCGCCATAGTAAGCGTCTCTTCTCTGATCCTCGTTGCAATGGTGGTCGCCGTTACTGTCGGCGTCAACAGCTCACCCTACGGTGACGATCACGGTGAGGGTCATGGTGACGGTCACGGTGACGGAGGTGCCGAGATTAAGACATCGACGAAAGCAATTCAATCCATTTGTCGACCAACGGATTACAAAGAAACCTGCGAGCAGAGCCTGATGGCCGAAGCCAGTAACACGACGGACCCGAAAGAGCTCGCGAAAGCCGGCTTCAAAGTCGCCGTCGAATATATAAACACGGCGATTAGAAATTCCACCACACTCAAAGAGCTGGCCAAGGAACCAAGAACCAACCAAGCCCTTCAGAACTGCCGCGAGCTTCTGGAATACGCCATCGACGATTTAACCCAATCTTTCGAAAGAATCGGCACCTTCGAAATGTCGAAACTCGACCAGTTCGTCAACGATTTGAAGATCTGGCTTAGTGGGGCCCTCACGTACGAGCAGACTTGTTTGGACGGATTCGAAAACACGACCGGTGATGCCGGTCAGAAAATGCAGCAGTTTTTGAAGTCCGCTCAGGAAATGACGAGTAACGGTCTTGCGATCGTAACTGAATTGTCCACTATTATGGGATCACTTAATCAAATGCCGGGAGCGACGCCGGGAGCGACGGGACGACGGCTGTTGGACGAGGAAATGCCGTCGTGGGTGAGCGAAGGAAAGAGGCGGCTGATGCAGGCGGGACCGGGGGGGCTGAAGCCGGACGTGGTGGTGGCTCAAGATGGAAGTGGAAAATACAAGACGATCAGCGCGGCTTTGGTTGATGTGCCGAAGAAGAGTAATAAGACATTTGTGATTTATGTGAAGGCTGGAATTTATAAGGAAATCGTTGTTGTTCCTAAGGATGTGACTCATCTTACGATGTACGGCGATGGCCCAACCAAGACTGTCGTCACTGGTAGTCTCAACTTTATCGATGGCATTCAAACATTCAAGACCGCAACTTTCTCTACGATCGGAACTGATTTCTTCGCTAAAGACATGGGATTCGTGAACAGTGCTGGAGCTGCAAAGCACCAAGCCGTGGCGTTACGAGTCCAATCCGATAGAGCGATCTTTTACAACTGCCAAATGGATGGGTATCAAGATACTCTCTACGCCCACGCCCATCGCCAGTTCTACCGTGACTGTTCGATCAGTGGCACCATCGACTTCGTCTTCGGCAACGCGGCCGCCGTCTTCCAGAACTGCAAACTAATCGTCCGAAAGCCAATGGATAACCAACAATGCATCGTCACGGCTCAAGGCCGGCTCAACCGCCGCGAACCGACCGCCCTAGTGTTCCAAGGGTGCCACTTCATGGGCGATCCGGCTTACTTGCCATTCAAGGCCGTCAACAAGGCTTACTTGGGGCGACCCTGGAAGGAATATTCACGAACCCTCATTATCGGGTCTCAAATCGACGATTTGATCCAGCCCGAGGGATGGTTGCCTTGGGGTGGCAATTTTGGGCTGAACACGCTGTTCTACGCGGAGGTTCAGAACAAGGGACCAGGGGCCGATCTTAGCAAGAGGGTGAAGTGGCGAGGAATTAAAACTATTACGCCAGATCACGCCGCTAAATTCACTCCCAGGGTATTTATCGACGGCGATGCTTGGATTAAGCCCAAGGGAATTCCGTATGCTTCTGGAATGATGTAA

Coding sequence (CDS)

ATGTCGAACGCCGAAGGTGACAAAAAGAAGAAGAATATCGCCATAGTAAGCGTCTCTTCTCTGATCCTCGTTGCAATGGTGGTCGCCGTTACTGTCGGCGTCAACAGCTCACCCTACGGTGACGATCACGGTGAGGGTCATGGTGACGGTCACGGTGACGGAGGTGCCGAGATTAAGACATCGACGAAAGCAATTCAATCCATTTGTCGACCAACGGATTACAAAGAAACCTGCGAGCAGAGCCTGATGGCCGAAGCCAGTAACACGACGGACCCGAAAGAGCTCGCGAAAGCCGGCTTCAAAGTCGCCGTCGAATATATAAACACGGCGATTAGAAATTCCACCACACTCAAAGAGCTGGCCAAGGAACCAAGAACCAACCAAGCCCTTCAGAACTGCCGCGAGCTTCTGGAATACGCCATCGACGATTTAACCCAATCTTTCGAAAGAATCGGCACCTTCGAAATGTCGAAACTCGACCAGTTCGTCAACGATTTGAAGATCTGGCTTAGTGGGGCCCTCACGTACGAGCAGACTTGTTTGGACGGATTCGAAAACACGACCGGTGATGCCGGTCAGAAAATGCAGCAGTTTTTGAAGTCCGCTCAGGAAATGACGAGTAACGGTCTTGCGATCGTAACTGAATTGTCCACTATTATGGGATCACTTAATCAAATGCCGGGAGCGACGCCGGGAGCGACGGGACGACGGCTGTTGGACGAGGAAATGCCGTCGTGGGTGAGCGAAGGAAAGAGGCGGCTGATGCAGGCGGGACCGGGGGGGCTGAAGCCGGACGTGGTGGTGGCTCAAGATGGAAGTGGAAAATACAAGACGATCAGCGCGGCTTTGGTTGATGTGCCGAAGAAGAGTAATAAGACATTTGTGATTTATGTGAAGGCTGGAATTTATAAGGAAATCGTTGTTGTTCCTAAGGATGTGACTCATCTTACGATGTACGGCGATGGCCCAACCAAGACTGTCGTCACTGGTAGTCTCAACTTTATCGATGGCATTCAAACATTCAAGACCGCAACTTTCTCTACGATCGGAACTGATTTCTTCGCTAAAGACATGGGATTCGTGAACAGTGCTGGAGCTGCAAAGCACCAAGCCGTGGCGTTACGAGTCCAATCCGATAGAGCGATCTTTTACAACTGCCAAATGGATGGGTATCAAGATACTCTCTACGCCCACGCCCATCGCCAGTTCTACCGTGACTGTTCGATCAGTGGCACCATCGACTTCGTCTTCGGCAACGCGGCCGCCGTCTTCCAGAACTGCAAACTAATCGTCCGAAAGCCAATGGATAACCAACAATGCATCGTCACGGCTCAAGGCCGGCTCAACCGCCGCGAACCGACCGCCCTAGTGTTCCAAGGGTGCCACTTCATGGGCGATCCGGCTTACTTGCCATTCAAGGCCGTCAACAAGGCTTACTTGGGGCGACCCTGGAAGGAATATTCACGAACCCTCATTATCGGGTCTCAAATCGACGATTTGATCCAGCCCGAGGGATGGTTGCCTTGGGGTGGCAATTTTGGGCTGAACACGCTGTTCTACGCGGAGGTTCAGAACAAGGGACCAGGGGCCGATCTTAGCAAGAGGGTGAAGTGGCGAGGAATTAAAACTATTACGCCAGATCACGCCGCTAAATTCACTCCCAGGGTATTTATCGACGGCGATGCTTGGATTAAGCCCAAGGGAATTCCGTATGCTTCTGGAATGATGTAA

Protein sequence

MSNAEGDKKKKNIAIVSVSSLILVAMVVAVTVGVNSSPYGDDHGEGHGDGHGDGGAEIKTSTKAIQSICRPTDYKETCEQSLMAEASNTTDPKELAKAGFKVAVEYINTAIRNSTTLKELAKEPRTNQALQNCRELLEYAIDDLTQSFERIGTFEMSKLDQFVNDLKIWLSGALTYEQTCLDGFENTTGDAGQKMQQFLKSAQEMTSNGLAIVTELSTIMGSLNQMPGATPGATGRRLLDEEMPSWVSEGKRRLMQAGPGGLKPDVVVAQDGSGKYKTISAALVDVPKKSNKTFVIYVKAGIYKEIVVVPKDVTHLTMYGDGPTKTVVTGSLNFIDGIQTFKTATFSTIGTDFFAKDMGFVNSAGAAKHQAVALRVQSDRAIFYNCQMDGYQDTLYAHAHRQFYRDCSISGTIDFVFGNAAAVFQNCKLIVRKPMDNQQCIVTAQGRLNRREPTALVFQGCHFMGDPAYLPFKAVNKAYLGRPWKEYSRTLIIGSQIDDLIQPEGWLPWGGNFGLNTLFYAEVQNKGPGADLSKRVKWRGIKTITPDHAAKFTPRVFIDGDAWIKPKGIPYASGMM

Homology

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BLAST of Lag0031495 vs. NCBI nr
Match: XP_008458409.1 (PREDICTED: probable pectinesterase/pectinesterase inhibitor 21 [Cucumis melo])

HSP 1 Score: 959.5 bits (2479), Expect = 1.3e-275
Identity = 471/578 (81.49%), Postives = 522/578 (90.31%), Query Frame = 0


Query: 2   SNAEGDKKKKNIAIVSVSSLILVAMVVAVTVGVNSSPYGDDHGEGHGDGHGDGGAEIKTS 61
           +NAEGDKKKK IAI+SVSSLILVAMVVAVTVG+N++PY ++HGE  GD       EI +S
Sbjct: 6   ANAEGDKKKKKIAIISVSSLILVAMVVAVTVGINNAPY-EEHGE--GDQGSKTTPEISSS 65

Query: 62  TKAIQSICRPTDYKETCEQSLMAEASNTTDPKELAKAGFKVAVEYINTAIRNSTTLKELA 121
           TKAIQ+IC+PTDYKETCE+SLMAEA NTTDPKEL K GFK+A   +N AIRNSTTL+EL+
Sbjct: 66  TKAIQAICQPTDYKETCEKSLMAEAGNTTDPKELVKVGFKIATRSLNEAIRNSTTLQELS 125

Query: 122 KEPRTNQALQNCRELLEYAIDDLTQSFERIGTFEMSKLDQFVNDLKIWLSGALTYEQTCL 181
           K+PRTNQALQNCRELLEYAIDDL +SFERIGTF+MSKLD+FV DLKIWLSGALTYEQTCL
Sbjct: 126 KDPRTNQALQNCRELLEYAIDDLNESFERIGTFQMSKLDEFVADLKIWLSGALTYEQTCL 185

Query: 182 DGFENTTGDAGQKMQQFLKSAQEMTSNGLAIVTELSTIMGSLNQMPGATPGATGRRLL-- 241
           DGFENTTGDAG +MQ+FLKSAQEMT+NGL IV ELSTI+GSL       PG TGRRLL  
Sbjct: 186 DGFENTTGDAGTRMQEFLKSAQEMTTNGLGIVNELSTILGSLQ-----IPGMTGRRLLED 245

Query: 242 -DEEMPSWVSEGKRRLMQAGPGGLKPDVVVAQDGSGKYKTISAALVDVPKKSNKTFVIYV 301
            D EMPSW+S+GKRRLMQAG  G+KPD++VAQDGSGKYKTI+AAL DVP KSNKTFVIYV
Sbjct: 246 DDHEMPSWISDGKRRLMQAGAAGMKPDLIVAQDGSGKYKTINAALADVPLKSNKTFVIYV 305

Query: 302 KAGIYKEIVVVPKDVTHLTMYGDGPTKTVVTGSLNFIDGIQTFKTATFSTIGTDFFAKDM 361
           KAGIYKEIVV+PK +THLTMYGDGPTKTVVTGSLNFIDGIQTFKTATFS IG +F+A+DM
Sbjct: 306 KAGIYKEIVVIPKHMTHLTMYGDGPTKTVVTGSLNFIDGIQTFKTATFSAIGANFYARDM 365

Query: 362 GFVNSAGAAKHQAVALRVQSDRAIFYNCQMDGYQDTLYAHAHRQFYRDCSISGTIDFVFG 421
           GF N+AG+AKHQAVALRVQSDR+IFYNCQ+DGYQDTLYAHAHRQFYRDC+ISGTIDFVFG
Sbjct: 366 GFENTAGSAKHQAVALRVQSDRSIFYNCQIDGYQDTLYAHAHRQFYRDCTISGTIDFVFG 425

Query: 422 NAAAVFQNCKLIVRKPMDNQQCIVTAQGRLNRREPTALVFQGCHFMGDPAYLPFKAVNKA 481
           NAA  FQNCKL+VRKPMDNQQCIVTA GR+NR+EPTAL+FQGCHFMGDPAYLPFK++NKA
Sbjct: 426 NAATNFQNCKLVVRKPMDNQQCIVTAHGRMNRKEPTALIFQGCHFMGDPAYLPFKSINKA 485

Query: 482 YLGRPWKEYSRTLIIGSQIDDLIQPEGWLPWGGNFGLNTLFYAEVQNKGPGADLSKRVKW 541
           YLGRPWK+YSRT+IIGSQIDD+IQPEGWLPW G+FGLNTLFYAEVQNKGPGA  +KRVKW
Sbjct: 486 YLGRPWKQYSRTIIIGSQIDDIIQPEGWLPWMGDFGLNTLFYAEVQNKGPGAVETKRVKW 545

Query: 542 RGIKTITPDHAAKFTPRVFIDGDAWIKPKGIPYASGMM 577
           RGIK ITP HAA FTPR FIDGDAWI  KGIPY+SGMM
Sbjct: 546 RGIKHITPQHAADFTPRRFIDGDAWIPAKGIPYSSGMM 575

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BLAST of Lag0031495 vs. NCBI nr
Match: XP_004150503.1 (probable pectinesterase/pectinesterase inhibitor 21 [Cucumis sativus] >KGN47423.1 hypothetical protein Csa_021732 [Cucumis sativus])

HSP 1 Score: 955.3 bits (2468), Expect = 2.4e-274
Identity = 472/579 (81.52%), Postives = 520/579 (89.81%), Query Frame = 0


Query: 2   SNAEGDKKKKNIAIVSVSSLILVAMVVAVTVGVNSSPYGDDHGEGHGDGHG-DGGAEIKT 61
           +NAEGDKKKK IAI+SVSSLILVAMVVAVTVGVN++P+G++HG+   D HG +   EI +
Sbjct: 6   ANAEGDKKKKKIAIISVSSLILVAMVVAVTVGVNNAPHGEEHGD---DNHGSETTPEITS 65

Query: 62  STKAIQSICRPTDYKETCEQSLMAEASNTTDPKELAKAGFKVAVEYINTAIRNSTTLKEL 121
           STKAIQ+IC+PTDYKETCE+SL AEA NTTDPKEL K GFK+A   +N AI+NSTTLKEL
Sbjct: 66  STKAIQAICQPTDYKETCEKSLEAEAGNTTDPKELVKVGFKIATRSLNEAIKNSTTLKEL 125

Query: 122 AKEPRTNQALQNCRELLEYAIDDLTQSFERIGTFEMSKLDQFVNDLKIWLSGALTYEQTC 181
           AK+PRTNQALQNCRELLEYAIDDL QSFERIGTF+MSKLD FV DLKIWLSGALTYEQTC
Sbjct: 126 AKDPRTNQALQNCRELLEYAIDDLNQSFERIGTFQMSKLDDFVADLKIWLSGALTYEQTC 185

Query: 182 LDGFENTTGDAGQKMQQFLKSAQEMTSNGLAIVTELSTIMGSLNQMPGATPGATGRRLL- 241
           LDGFENTTGDAG +MQ+FLKSAQ+MT+NGL IV ELSTI+GSL       PG +GRRLL 
Sbjct: 186 LDGFENTTGDAGVRMQEFLKSAQQMTTNGLGIVNELSTILGSLQ-----LPGMSGRRLLE 245

Query: 242 --DEEMPSWVSEGKRRLMQAGPGGLKPDVVVAQDGSGKYKTISAALVDVPKKSNKTFVIY 301
             D EMPSWVS+GKRRLMQAG   +KPD+VVAQDGSGKYKTI+AAL DVP KSNKTFVI+
Sbjct: 246 DDDREMPSWVSDGKRRLMQAGAAAMKPDLVVAQDGSGKYKTINAALADVPLKSNKTFVIH 305

Query: 302 VKAGIYKEIVVVPKDVTHLTMYGDGPTKTVVTGSLNFIDGIQTFKTATFSTIGTDFFAKD 361
           VKAGIYKEIVV+PK +THLTMYGDGPTKTVVTGSLNFIDGIQTFKTATFS IG +F+A+D
Sbjct: 306 VKAGIYKEIVVIPKHMTHLTMYGDGPTKTVVTGSLNFIDGIQTFKTATFSAIGANFYARD 365

Query: 362 MGFVNSAGAAKHQAVALRVQSDRAIFYNCQMDGYQDTLYAHAHRQFYRDCSISGTIDFVF 421
           MGF N+AGAAKHQAVALRVQSDR+IF+NCQ+DGYQDTLYAHAHRQFYRDC+ISGTIDFVF
Sbjct: 366 MGFENTAGAAKHQAVALRVQSDRSIFFNCQIDGYQDTLYAHAHRQFYRDCTISGTIDFVF 425

Query: 422 GNAAAVFQNCKLIVRKPMDNQQCIVTAQGRLNRREPTALVFQGCHFMGDPAYLPFKAVNK 481
           GNAA  FQNCKL+VRKP+DNQQCIVTA GRLNR+EPTAL+FQ CHFMGDPAYLPFKA+NK
Sbjct: 426 GNAATNFQNCKLVVRKPLDNQQCIVTAHGRLNRKEPTALIFQSCHFMGDPAYLPFKAINK 485

Query: 482 AYLGRPWKEYSRTLIIGSQIDDLIQPEGWLPWGGNFGLNTLFYAEVQNKGPGADLSKRVK 541
           AYLGRPWKEYSRT+IIGS IDD+IQPEGWLPW G+FGLNTLFYAEVQNKG GAD SKRVK
Sbjct: 486 AYLGRPWKEYSRTIIIGSTIDDIIQPEGWLPWMGDFGLNTLFYAEVQNKGAGADESKRVK 545

Query: 542 WRGIKTITPDHAAKFTPRVFIDGDAWIKPKGIPYASGMM 577
           WRGIK ITP HAA FTPR FIDGDAWI  KGIPY+SGMM
Sbjct: 546 WRGIKHITPQHAADFTPRRFIDGDAWIPAKGIPYSSGMM 576

[Close]

BLAST of Lag0031495 vs. NCBI nr
Match: XP_038875843.1 (probable pectinesterase/pectinesterase inhibitor 21 [Benincasa hispida])

HSP 1 Score: 946.0 bits (2444), Expect = 1.5e-271
Identity = 463/578 (80.10%), Postives = 518/578 (89.62%), Query Frame = 0


Query: 2   SNAEGDKKKKNIAIVSVSSLILVAMVVAVTVGVNSSPYGDDHGEGHGDGHGDGGAEIKTS 61
           +NAEGDKKKK +AI+SVSSLILVAMVVAVTVGVN++P      E HGDGHG    EI ++
Sbjct: 6   ANAEGDKKKKKMAIISVSSLILVAMVVAVTVGVNNAP-----KEEHGDGHGGETHEISST 65

Query: 62  TKAIQSICRPTDYKETCEQSLMAEASNTTDPKELAKAGFKVAVEYINTAIRNSTTLKELA 121
           TKAIQ+IC+PTDYK+ CE++LMAEA NTTDPKELAK G ++A   +N AIRNSTTL+ELA
Sbjct: 66  TKAIQAICQPTDYKDVCEKNLMAEAGNTTDPKELAKVGLRIATRNLNEAIRNSTTLQELA 125

Query: 122 KEPRTNQALQNCRELLEYAIDDLTQSFERIGTFEMSKLDQFVNDLKIWLSGALTYEQTCL 181
           K+PRTNQALQNCRELLEYAIDDL +SFERIGTF+MSKLD+FV DLKIWLSGALTYEQTCL
Sbjct: 126 KDPRTNQALQNCRELLEYAIDDLNESFERIGTFQMSKLDEFVADLKIWLSGALTYEQTCL 185

Query: 182 DGFENTTGDAGQKMQQFLKSAQEMTSNGLAIVTELSTIMGSLNQMPGATPGATGRRLLD- 241
           DGFENTTGDAG +MQ+FLKS+Q+MT+NGLAIV ELS ++GSL       PG TGRRLL+ 
Sbjct: 186 DGFENTTGDAGVRMQEFLKSSQQMTTNGLAIVNELSGMLGSLQ-----IPGMTGRRLLED 245

Query: 242 --EEMPSWVSEGKRRLMQAGPGGLKPDVVVAQDGSGKYKTISAALVDVPKKSNKTFVIYV 301
             +EMPSWVSEGKRRLMQA    +KPD+VVAQDGSGKYKTI+AAL DVP KSNKTFVIYV
Sbjct: 246 DADEMPSWVSEGKRRLMQAAAASMKPDLVVAQDGSGKYKTINAALADVPLKSNKTFVIYV 305

Query: 302 KAGIYKEIVVVPKDVTHLTMYGDGPTKTVVTGSLNFIDGIQTFKTATFSTIGTDFFAKDM 361
           KAGIYKEIVV+PK +THLTMYGDGPTKT+VTGSLNFIDGIQTFKTATF+ IG +F+A+DM
Sbjct: 306 KAGIYKEIVVIPKAMTHLTMYGDGPTKTIVTGSLNFIDGIQTFKTATFAAIGANFYARDM 365

Query: 362 GFVNSAGAAKHQAVALRVQSDRAIFYNCQMDGYQDTLYAHAHRQFYRDCSISGTIDFVFG 421
           GF N+AGAAKHQAVALRVQSDR+IFYNC++DGYQDTLYAHAHRQFYRDC ISGTIDFVFG
Sbjct: 366 GFENTAGAAKHQAVALRVQSDRSIFYNCKIDGYQDTLYAHAHRQFYRDCIISGTIDFVFG 425

Query: 422 NAAAVFQNCKLIVRKPMDNQQCIVTAQGRLNRREPTALVFQGCHFMGDPAYLPFKAVNKA 481
           NAA +FQNCKL+VRKPMDNQQCIVTA GRLNR+EPTAL+FQ CHFMGDPAYLPFK +NKA
Sbjct: 426 NAATIFQNCKLVVRKPMDNQQCIVTAHGRLNRKEPTALIFQSCHFMGDPAYLPFKNINKA 485

Query: 482 YLGRPWKEYSRTLIIGSQIDDLIQPEGWLPWGGNFGLNTLFYAEVQNKGPGADLSKRVKW 541
           YLGRPWK+YSRT+IIGSQIDD+IQPEGWLPW G+FGLNTLFYAEVQNKGPGAD SKRVKW
Sbjct: 486 YLGRPWKQYSRTIIIGSQIDDIIQPEGWLPWMGDFGLNTLFYAEVQNKGPGADESKRVKW 545

Query: 542 RGIKTITPDHAAKFTPRVFIDGDAWIKPKGIPYASGMM 577
           RGI+ ITP HAA +TPR FIDGDAWI  KGIPY+SGMM
Sbjct: 546 RGIRHITPQHAADYTPRRFIDGDAWIPAKGIPYSSGMM 573

[Close]

BLAST of Lag0031495 vs. NCBI nr
Match: XP_023549205.1 (probable pectinesterase/pectinesterase inhibitor 21 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 933.3 bits (2411), Expect = 9.8e-268
Identity = 453/579 (78.24%), Postives = 515/579 (88.95%), Query Frame = 0


Query: 3   NAEGDKKKKNIAIVSVSSLILVAMVVAVTVGVNSSPYGD-DHGEGHGDG----HGDGGAE 62
           +AEGDKKKK IA++SVSSLILVAMVVAVTVGVN++P  D D G G G G    HGD G +
Sbjct: 6   DAEGDKKKKKIAVISVSSLILVAMVVAVTVGVNNAPQEDLDDGGGGGGGDAHDHGDDGEK 65

Query: 63  IKTSTKAIQSICRPTDYKETCEQSLMAEASNTTDPKELAKAGFKVAVEYINTAIRNSTTL 122
           + +STKAI++IC+PTDYKETCE+SLM EA NTTDPKEL KAGFK+AVE +N AIRNSTTL
Sbjct: 66  VSSSTKAIKTICQPTDYKETCEESLMVEAGNTTDPKELVKAGFKIAVEKLNAAIRNSTTL 125

Query: 123 KELAKEPRTNQALQNCRELLEYAIDDLTQSFERIGTFEMSKLDQFVNDLKIWLSGALTYE 182
           +ELA++PR NQALQNCRELLEYAIDDL  S+ RIGTF +SK ++FVNDLKIWLS ALTYE
Sbjct: 126 QELAQDPRANQALQNCRELLEYAIDDLHDSYHRIGTFHVSKFEEFVNDLKIWLSAALTYE 185

Query: 183 QTCLDGFENTTGDAGQKMQQFLKSAQEMTSNGLAIVTELSTIMGSLNQMPGATPGATGRR 242
           QTCLDGFENTTGD+G++M++FLKSAQEMT+NGL IV+ELS I+G+L+      P ATGRR
Sbjct: 186 QTCLDGFENTTGDSGERMKEFLKSAQEMTTNGLGIVSELSNILGTLH-----APAATGRR 245

Query: 243 LLDEEMPSWVSEGKRRLMQAGPGGLKPDVVVAQDGSGKYKTISAALVDVPKKSNKTFVIY 302
           LLD +MP WVS+GKRRLMQ G G +KPD+VVAQDGSGKYKTI+ ALVDVP KSNKTFVIY
Sbjct: 246 LLDTDMPLWVSDGKRRLMQMGTGAIKPDLVVAQDGSGKYKTINEALVDVPMKSNKTFVIY 305

Query: 303 VKAGIYKEIVVVPKDVTHLTMYGDGPTKTVVTGSLNFIDGIQTFKTATFSTIGTDFFAKD 362
           VKAGIYKEIVV+PK +THLTMYGDGPTK+VVTG+LNF+DGIQTFKTATF+ IG +FF +D
Sbjct: 306 VKAGIYKEIVVIPKHMTHLTMYGDGPTKSVVTGNLNFVDGIQTFKTATFAAIGANFFVRD 365

Query: 363 MGFVNSAGAAKHQAVALRVQSDRAIFYNCQMDGYQDTLYAHAHRQFYRDCSISGTIDFVF 422
           MGFVN+AG  KHQAVALRVQSDRAI YNCQ+DGYQDTLYAHA+RQFYRDC ISGTIDFVF
Sbjct: 366 MGFVNTAGPTKHQAVALRVQSDRAIIYNCQIDGYQDTLYAHANRQFYRDCIISGTIDFVF 425

Query: 423 GNAAAVFQNCKLIVRKPMDNQQCIVTAQGRLNRREPTALVFQGCHFMGDPAYLPFKAVNK 482
           GN+AA+FQNCKL+VRKP+DNQQCIVTA GRLNRREPTAL+FQGCHFMGDPAYLPFK VNK
Sbjct: 426 GNSAAIFQNCKLVVRKPLDNQQCIVTAHGRLNRREPTALIFQGCHFMGDPAYLPFKKVNK 485

Query: 483 AYLGRPWKEYSRTLIIGSQIDDLIQPEGWLPWGGNFGLNTLFYAEVQNKGPGADLSKRVK 542
           AYLGRPWK+YSRT+I+GSQIDDLIQPEGWLPW G+F LNTLFYAEVQN+GPGAD S RVK
Sbjct: 486 AYLGRPWKQYSRTIIMGSQIDDLIQPEGWLPWMGDFALNTLFYAEVQNRGPGADQSHRVK 545

Query: 543 WRGIKTITPDHAAKFTPRVFIDGDAWIKPKGIPYASGMM 577
           WRGI+ ITP HAA +T R FIDGDAWI  +G+PY+SGMM
Sbjct: 546 WRGIRHITPQHAAAYTARRFIDGDAWIPVRGVPYSSGMM 579

[Close]

BLAST of Lag0031495 vs. NCBI nr
Match: XP_023006255.1 (probable pectinesterase/pectinesterase inhibitor 21 [Cucurbita maxima])

HSP 1 Score: 927.9 bits (2397), Expect = 4.1e-266
Identity = 453/600 (75.50%), Postives = 518/600 (86.33%), Query Frame = 0


Query: 3   NAEGDKKKKNIAIVSVSSLILVAMVVAVTVGVNSSPYGD-----------DHGEGHGD-- 62
           +AEGDKKKK IA++SVSSLILVAMVVAVTVGVN++P+ D           DHG+  GD  
Sbjct: 6   DAEGDKKKKKIAVISVSSLILVAMVVAVTVGVNNAPHEDLDDGGGGGDLHDHGDDGGDVH 65

Query: 63  -------------GHGDGGAEIKTSTKAIQSICRPTDYKETCEQSLMAEASNTTDPKELA 122
                         HGD G +I +STKAI++IC+PTDYKETCE+SLM EA NTTD KEL 
Sbjct: 66  DHGDYGGGGGDAHDHGDDGEKISSSTKAIKTICQPTDYKETCEESLMVEAGNTTDLKELV 125

Query: 123 KAGFKVAVEYINTAIRNSTTLKELAKEPRTNQALQNCRELLEYAIDDLTQSFERIGTFEM 182
           KAGFK+AVE +N AIRNSTTL+ELAK+PR NQALQNCRELLEYAIDDL  S+ RIGTF +
Sbjct: 126 KAGFKIAVEKLNAAIRNSTTLQELAKDPRANQALQNCRELLEYAIDDLHDSYHRIGTFHV 185

Query: 183 SKLDQFVNDLKIWLSGALTYEQTCLDGFENTTGDAGQKMQQFLKSAQEMTSNGLAIVTEL 242
           SK ++FVNDLKIWLS ALTYEQTCLDGFENTTGD+G++M++FLKSAQEMT+NGL IV+EL
Sbjct: 186 SKFEEFVNDLKIWLSAALTYEQTCLDGFENTTGDSGERMKEFLKSAQEMTTNGLGIVSEL 245

Query: 243 STIMGSLNQMPGATPGATGRRLLDEEMPSWVSEGKRRLMQAGPGGLKPDVVVAQDGSGKY 302
           S I+G+L++     P ATGRRLLD +MP WVS+GKRRLMQ   G +KPD+VVAQDGSGKY
Sbjct: 246 SNILGTLHE-----PAATGRRLLDTDMPLWVSDGKRRLMQLETGAIKPDLVVAQDGSGKY 305

Query: 303 KTISAALVDVPKKSNKTFVIYVKAGIYKEIVVVPKDVTHLTMYGDGPTKTVVTGSLNFID 362
           KTI+ ALVDVP KSNKTFVIYVKAGIYKEIVV+PK +THLTMYGDGPTK+VVTG+LNF+D
Sbjct: 306 KTINEALVDVPMKSNKTFVIYVKAGIYKEIVVIPKHMTHLTMYGDGPTKSVVTGNLNFVD 365

Query: 363 GIQTFKTATFSTIGTDFFAKDMGFVNSAGAAKHQAVALRVQSDRAIFYNCQMDGYQDTLY 422
           GIQTFKTATF+ IG +FF +DMGFVNSAG  KHQAVALR+QSDRAI YNCQ+DG+QDTLY
Sbjct: 366 GIQTFKTATFAAIGANFFVRDMGFVNSAGPTKHQAVALRIQSDRAIIYNCQIDGFQDTLY 425

Query: 423 AHAHRQFYRDCSISGTIDFVFGNAAAVFQNCKLIVRKPMDNQQCIVTAQGRLNRREPTAL 482
           AHA+RQFYRDC+ISGTIDFVFGN+AA+FQNCKL+VRKP+DNQQCIVTA GRLNRREPTAL
Sbjct: 426 AHANRQFYRDCTISGTIDFVFGNSAAIFQNCKLVVRKPLDNQQCIVTAHGRLNRREPTAL 485

Query: 483 VFQGCHFMGDPAYLPFKAVNKAYLGRPWKEYSRTLIIGSQIDDLIQPEGWLPWGGNFGLN 542
           +FQGCHFMGDPAYLPFK VNKAYLGRPWK+YSRT+I+GSQIDDLIQPEGWLPW G+FGLN
Sbjct: 486 IFQGCHFMGDPAYLPFKKVNKAYLGRPWKQYSRTIIMGSQIDDLIQPEGWLPWMGDFGLN 545

Query: 543 TLFYAEVQNKGPGADLSKRVKWRGIKTITPDHAAKFTPRVFIDGDAWIKPKGIPYASGMM 577
           TLFYAEVQN+GPGAD S RVKWRGI+ ITP HAA +T R FIDGDAWI  +G+PY+SGMM
Sbjct: 546 TLFYAEVQNRGPGADQSHRVKWRGIRHITPQHAASYTARRFIDGDAWIPVRGVPYSSGMM 600

[Close]

BLAST of Lag0031495 vs. ExPASy Swiss-Prot
Match: Q8GX86 (Probable pectinesterase/pectinesterase inhibitor 21 OS=Arabidopsis thaliana OX=3702 GN=PME21 PE=2 SV=2)

HSP 1 Score: 648.3 bits (1671), Expect = 8.3e-185
Identity = 306/571 (53.59%), Postives = 417/571 (73.03%), Query Frame = 0


Query: 8   KKKKNIAIVSVSSLILVAMVVAVTVGVNSSPYGDDHGEGHGDGHGDGGAEIKTSTKAIQS 67
           K+K+   ++++SS++L++MVVAVTVGV+ + +         DG   G AE+  S KA++ 
Sbjct: 10  KRKRRYIVITISSVLLISMVVAVTVGVSLNKH---------DGDSKGKAEVNASVKAVKD 69

Query: 68  ICRPTDYKETCEQSLMAEASNTTDPKELAKAGFKVAVEYINTAIRNSTTLKELAKEPRTN 127
           +C PTDY++TCE +L+    NTTDP EL K  F V ++ I  A + S T+ EL K+ RT 
Sbjct: 70  VCAPTDYRKTCEDTLIKNGKNTTDPMELVKTAFNVTMKQITDAAKKSQTIMELQKDSRTR 129

Query: 128 QALQNCRELLEYAIDDLTQSFERIGTFEMSKLDQFVNDLKIWLSGALTYEQTCLDGFENT 187
            AL  C+EL++YA+D+L+ SFE +G FE   LD+ + +L+IWLS A+++E+TCL+GF+ T
Sbjct: 130 MALDQCKELMDYALDELSNSFEELGKFEFHLLDEALINLRIWLSAAISHEETCLEGFQGT 189

Query: 188 TGDAGQKMQQFLKSAQEMTSNGLAIVTELSTIMGSLNQMPGATPGATGRRLLDEEMPSWV 247
            G+AG+ M++ LK+A E+T NGLAI++E+S  +G +       PG   RRLL E  PSWV
Sbjct: 190 QGNAGETMKKALKTAIELTHNGLAIISEMSNFVGQMQ-----IPGLNSRRLLAEGFPSWV 249

Query: 248 SEGKRRLMQAGP--GGLKPDVVVAQDGSGKYKTISAALVDVPKKSNKTFVIYVKAGIYKE 307
            +  R+L+QA      +KPD+VVAQDGSG+YKTI+ AL  VPKK N TFV+++KAG+YKE
Sbjct: 250 DQRGRKLLQAAAAYSDVKPDIVVAQDGSGQYKTINEALQFVPKKRNTTFVVHIKAGLYKE 309

Query: 308 IVVVPKDVTHLTMYGDGPTKTVVTGSLNFIDGIQTFKTATFSTIGTDFFAKDMGFVNSAG 367
            V V K ++HL   GDGP KT+++G+ N+ DGI T++TAT + +G  F AK++GF N+AG
Sbjct: 310 YVQVNKTMSHLVFIGDGPDKTIISGNKNYKDGITTYRTATVAIVGNYFIAKNIGFENTAG 369

Query: 368 AAKHQAVALRVQSDRAIFYNCQMDGYQDTLYAHAHRQFYRDCSISGTIDFVFGNAAAVFQ 427
           A KHQAVA+RVQSD +IF+NC+ DGYQDTLY H+HRQF+RDC+ISGTIDF+FG+AAAVFQ
Sbjct: 370 AIKHQAVAVRVQSDESIFFNCRFDGYQDTLYTHSHRQFFRDCTISGTIDFLFGDAAAVFQ 429

Query: 428 NCKLIVRKPMDNQQCIVTAQGRLNRREPTALVFQGCHFMGDPAYLPFKAVNKAYLGRPWK 487
           NC L+VRKP+ NQ C +TA GR + RE T  VFQGC   G+P YL  K  +KAYLGRPWK
Sbjct: 430 NCTLLVRKPLPNQACPITAHGRKDPRESTGFVFQGCTIAGEPDYLAVKETSKAYLGRPWK 489

Query: 488 EYSRTLIIGSQIDDLIQPEGWLPWGGNFGLNTLFYAEVQNKGPGADLSKRVKWRGIKTIT 547
           EYSRT+I+ + I D +QP+GW PW G+FGL TLFY+EVQN GPG+ L+ RV W GIKT++
Sbjct: 490 EYSRTIIMNTFIPDFVQPQGWQPWLGDFGLKTLFYSEVQNTGPGSALANRVTWAGIKTLS 549

Query: 548 PDHAAKFTPRVFIDGDAWIKPKGIPYASGMM 577
            +   KFTP  +I GD WI  KG+PY +G++
Sbjct: 550 EEDILKFTPAQYIQGDDWIPGKGVPYTTGLL 566

[Close]

BLAST of Lag0031495 vs. ExPASy Swiss-Prot
Match: Q3E8Z8 (Putative pectinesterase/pectinesterase inhibitor 28 OS=Arabidopsis thaliana OX=3702 GN=PME28 PE=2 SV=1)

HSP 1 Score: 639.8 bits (1649), Expect = 3.0e-182
Identity = 310/571 (54.29%), Postives = 409/571 (71.63%), Query Frame = 0


Query: 5   EGDKKKKNIAIVSVSSLILVAMVVAVTVGVNSSPYGDDHGEGHGDGHGDGGAEIKTSTKA 64
           E  K+KK   I+S+SS++L++MVVAVT+GV+ +           D  GD   EI TS KA
Sbjct: 8   EDAKRKKRYVIISISSVLLISMVVAVTIGVSVN---------KSDNAGD--EEITTSVKA 67

Query: 65  IQSICRPTDYKETCEQSLMAEASNTTDPKELAKAGFKVAVEYINTAIRNSTTLKELAKEP 124
           I+ +C PTDYKETCE +L  +A +T+DP EL K  F   ++ I+   + S T+ EL K+P
Sbjct: 68  IKDVCAPTDYKETCEDTLRKDAKDTSDPLELVKTAFNATMKQISDVAKKSQTMIELQKDP 127

Query: 125 RTNQALQNCRELLEYAIDDLTQSFERIGTFEMSKLDQFVNDLKIWLSGALTYEQTCLDGF 184
           R   AL  C+EL++YAI +L++SFE +G FE  K+D+ +  L+IWLS  +++EQTCLDGF
Sbjct: 128 RAKMALDQCKELMDYAIGELSKSFEELGKFEFHKVDEALVKLRIWLSATISHEQTCLDGF 187

Query: 185 ENTTGDAGQKMQQFLKSAQEMTSNGLAIVTELSTIMGSLNQMPGATPGATGRRLLDEEMP 244
           + T G+AG+ +++ LK+A ++T NGLA+VTE+S  +G +       P    RRLL +E P
Sbjct: 188 QGTQGNAGETIKKALKTAVQLTHNGLAMVTEMSNYLGQMQ-----IPEMNSRRLLSQEFP 247

Query: 245 SWVSEGKRRLMQAGPGGLKPDVVVAQDGSGKYKTISAALVDVPKKSNKTFVIYVKAGIYK 304
           SW+    RRL+ A    +KPD+VVAQDGSG+YKTI+ AL  VPKK N TFV+++K GIYK
Sbjct: 248 SWMDARARRLLNAPMSEVKPDIVVAQDGSGQYKTINEALNFVPKKKNTTFVVHIKEGIYK 307

Query: 305 EIVVVPKDVTHLTMYGDGPTKTVVTGSLNFIDGIQTFKTATFSTIGTDFFAKDMGFVNSA 364
           E V V + +THL   GDGP KTV++GS ++ DGI T+KTAT + +G  F AK++ F N+A
Sbjct: 308 EYVQVNRSMTHLVFIGDGPDKTVISGSKSYKDGITTYKTATVAIVGDHFIAKNIAFENTA 367

Query: 365 GAAKHQAVALRVQSDRAIFYNCQMDGYQDTLYAHAHRQFYRDCSISGTIDFVFGNAAAVF 424
           GA KHQAVA+RV +D +IFYNC+ DGYQDTLYAH+HRQFYRDC+ISGTIDF+FG+AAAVF
Sbjct: 368 GAIKHQAVAIRVLADESIFYNCKFDGYQDTLYAHSHRQFYRDCTISGTIDFLFGDAAAVF 427

Query: 425 QNCKLIVRKPMDNQQCIVTAQGRLNRREPTALVFQGCHFMGDPAYLPFKAVNKAYLGRPW 484
           QNC L+VRKP+ NQ C +TA GR + RE T  V QGC  +G+P YL  K  +K YLGRPW
Sbjct: 428 QNCTLLVRKPLLNQACPITAHGRKDPRESTGFVLQGCTIVGEPDYLAVKEQSKTYLGRPW 487

Query: 485 KEYSRTLIIGSQIDDLIQPEGWLPWGGNFGLNTLFYAEVQNKGPGADLSKRVKWRGIKTI 544
           KEYSRT+I+ + I D + PEGW PW G FGLNTLFY+EVQN GPGA ++KRV W GIK +
Sbjct: 488 KEYSRTIIMNTFIPDFVPPEGWQPWLGEFGLNTLFYSEVQNTGPGAAITKRVTWPGIKKL 547

Query: 545 TPDHAAKFTPRVFIDGDAWIKPKGIPYASGM 576
           + +   KFTP  +I GDAWI  KG+PY  G+
Sbjct: 548 SDEEILKFTPAQYIQGDAWIPGKGVPYILGL 562

[Close]

BLAST of Lag0031495 vs. ExPASy Swiss-Prot
Match: Q9FJ21 (Probable pectinesterase/pectinesterase inhibitor 58 OS=Arabidopsis thaliana OX=3702 GN=PME58 PE=2 SV=1)

HSP 1 Score: 582.0 bits (1499), Expect = 7.3e-165
Identity = 287/576 (49.83%), Postives = 397/576 (68.92%), Query Frame = 0


Query: 5   EGDKKKKNIAIVSVSSLILVAMVVAVTVGVNSSPYGDDHGEGHGDGHGDG--GAEIKTST 64
           +G+ KKK   I  V + +LV MVVA  VG+ +S             H +     +IKT+T
Sbjct: 4   DGELKKKKCIIAGVITALLVLMVVA--VGITTS---------RNTSHSEKIVPVQIKTAT 63

Query: 65  KAIQSICRPTDYKETCEQSLMAEASNTTDPKELAKAGFKVAVEYINTAIRNSTT--LKEL 124
            A++++C PTDYKETC  SLM  + ++T P +L K GF V +  I  +I+ ++     + 
Sbjct: 64  TAVEAVCAPTDYKETCVNSLMKASPDSTQPLDLIKLGFNVTIRSIEDSIKKASVELTAKA 123

Query: 125 AKEPRTNQALQNCRELLEYAIDDLTQSFERIGTFEMSKLDQFVNDLKIWLSGALTYEQTC 184
           A +  T  AL+ C +L+  A DDL +  +    F + +++ FV DL++WLSG++ Y+QTC
Sbjct: 124 ANDKDTKGALELCEKLMNDATDDLKKCLDNFDGFSIPQIEDFVEDLRVWLSGSIAYQQTC 183

Query: 185 LDGFENTTGDAGQKMQQFLKSAQEMTSNGLAIVTELSTIMGSLNQMPGATP--GATGRRL 244
           +D FE T     Q MQ+  K+++E+TSNGLA++T +S ++G  N + G T   G   R+L
Sbjct: 184 MDTFEETNSKLSQDMQKIFKTSRELTSNGLAMITNISNLLGEFN-VTGVTGDLGKYARKL 243

Query: 245 LDEE--MPSWVSEGKRRLMQAGPGGLKPDVVVAQDGSGKYKTISAALVDVPKKSNKTFVI 304
           L  E  +PSWV    RRLM A  GG+K +VVVA DGSG+YKTI+ AL  VPK + K FVI
Sbjct: 244 LSAEDGIPSWVGPNTRRLM-ATKGGVKANVVVAHDGSGQYKTINEALNAVPKANQKPFVI 303

Query: 305 YVKAGIYKEIVVVPKDVTHLTMYGDGPTKTVVTGSLNFIDG-IQTFKTATFSTIGTDFFA 364
           Y+K G+Y E V V K +TH+T  GDGPTKT +TGSLN+  G ++T+ TAT +  G +F A
Sbjct: 304 YIKQGVYNEKVDVTKKMTHVTFIGDGPTKTKITGSLNYYIGKVKTYLTATVAINGDNFTA 363

Query: 365 KDMGFVNSAGAAKHQAVALRVQSDRAIFYNCQMDGYQDTLYAHAHRQFYRDCSISGTIDF 424
           K++GF N+AG   HQAVALRV +D A+FYNCQ+DGYQDTLY H+HRQF+RDC++SGT+DF
Sbjct: 364 KNIGFENTAGPEGHQAVALRVSADLAVFYNCQIDGYQDTLYVHSHRQFFRDCTVSGTVDF 423

Query: 425 VFGNAAAVFQNCKLIVRKPMDNQQCIVTAQGRLNRREPTALVFQGCHFMGDPAYLPFKAV 484
           +FG+   V QNC ++VRKPM +Q C++TAQGR ++RE T LV Q CH  G+PAY+P K++
Sbjct: 424 IFGDGIVVLQNCNIVVRKPMKSQSCMITAQGRSDKRESTGLVLQNCHITGEPAYIPVKSI 483

Query: 485 NKAYLGRPWKEYSRTLIIGSQIDDLIQPEGWLPWGGNFGLNTLFYAEVQNKGPGADLSKR 544
           NKAYLGRPWKE+SRT+I+G+ IDD+I P GWLPW G+F LNTL+YAE +N GPG++ ++R
Sbjct: 484 NKAYLGRPWKEFSRTIIMGTTIDDVIDPAGWLPWNGDFALNTLYYAEYENNGPGSNQAQR 543

Query: 545 VKWRGIKTITPDHAAKFTPRVFIDGDAWIKPKGIPY 572
           VKW GIK ++P  A +FTP  F+ G+ WI P  +PY
Sbjct: 544 VKWPGIKKLSPKQALRFTPARFLRGNLWIPPNRVPY 566

[Close]

BLAST of Lag0031495 vs. ExPASy Swiss-Prot
Match: Q8GXA1 (Probable pectinesterase/pectinesterase inhibitor 23 OS=Arabidopsis thaliana OX=3702 GN=PME23 PE=2 SV=3)

HSP 1 Score: 541.6 bits (1394), Expect = 1.1e-152
Identity = 275/580 (47.41%), Postives = 388/580 (66.90%), Query Frame = 0


Query: 4   AEGDKKKKNIAIVSVSSLILVAMVVAVTVGVNSSPYGDDHGEGHGDGHGDGGAEIKTSTK 63
           ++GDKKKK I   SVS  +++ +V    V    SP  D++              I+ +TK
Sbjct: 3   SDGDKKKKFIVAGSVSGFLVIMVVSVAVVTSKHSPKDDEN-------------HIRKTTK 62

Query: 64  AIQSICRPTDYKETCEQSLMAEASNTTDPKELAKAGFKVAVEYINTAI-RNSTTLKELA- 123
           A+Q++C PTD+K+TC  SLM  + ++ DP +L K GFKV ++ IN ++ + S  +K  A 
Sbjct: 63  AVQAVCAPTDFKDTCVNSLMGASPDSDDPVDLIKLGFKVTIKSINESLEKASGDIKAKAD 122

Query: 124 KEPRTNQALQNCRELLEYAIDDLTQSFERIGTFEMSKLDQFVNDLKIWLSGALTYEQTCL 183
           K P    A + C +L+  AIDDL +  +    F + +++ FV DL++WLSG++ ++QTC+
Sbjct: 123 KNPEAKGAFELCEKLMIDAIDDLKKCMDH--GFSVDQIEVFVEDLRVWLSGSIAFQQTCM 182

Query: 184 DGFENTTGDAGQKMQQFLKSAQEMTSNGLAIVTELSTIMGSLNQMPGATPGAT--GRRLL 243
           D F     +  Q M +  K+++E++SN LA+VT +ST++ + N + G T       R+LL
Sbjct: 183 DSFGEIKSNLMQDMLKIFKTSRELSSNSLAMVTRISTLIPNSN-LTGLTGALAKYARKLL 242

Query: 244 --DEEMPSWVSEGKRRLMQA---GPGGLKPDVVVAQDGSGKYKTISAALVDVPKKSNKTF 303
             ++ +P+WV    RRLM A   GPG +K + VVAQDG+G++KTI+ AL  VPK +   F
Sbjct: 243 STEDSIPTWVGPEARRLMAAQGGGPGPVKANAVVAQDGTGQFKTITDALNAVPKGNKVPF 302

Query: 304 VIYVKAGIYKEIVVVPKDVTHLTMYGDGPTKTVVTGSLNF-IDGIQTFKTATFSTIGTDF 363
           +I++K GIYKE V V K + H+T  GDGP KT++TGSLNF I  ++TF TAT +  G  F
Sbjct: 303 IIHIKEGIYKEKVTVTKKMPHVTFIGDGPNKTLITGSLNFGIGKVKTFLTATITIEGDHF 362

Query: 364 FAKDMGFVNSAGAAKHQAVALRVQSDRAIFYNCQMDGYQDTLYAHAHRQFYRDCSISGTI 423
            AK++G  N+AG    QAVALRV +D A+F++CQ+DG+QDTLY H+HRQFYRDC++SGT+
Sbjct: 363 TAKNIGIENTAGPEGGQAVALRVSADYAVFHSCQIDGHQDTLYVHSHRQFYRDCTVSGTV 422

Query: 424 DFVFGNAAAVFQNCKLIVRKPMDNQQCIVTAQGRLNRREPTALVFQGCHFMGDPAYLPFK 483
           DF+FG+A  + QNCK++VRKP   Q C+VTAQGR N RE T LV  GCH  GDPAY+P K
Sbjct: 423 DFIFGDAKCILQNCKIVVRKPNKGQTCMVTAQGRSNVRESTGLVLHGCHITGDPAYIPMK 482

Query: 484 AVNKAYLGRPWKEYSRTLIIGSQIDDLIQPEGWLPWGGNFGLNTLFYAEVQNKGPGADLS 543
           +VNKAYLGRPWKE+SRT+I+ + IDD+I P GWLPW G+F L TL+YAE  N GPG++ +
Sbjct: 483 SVNKAYLGRPWKEFSRTIIMKTTIDDVIDPAGWLPWSGDFALKTLYYAEHMNTGPGSNQA 542

Query: 544 KRVKWRGIKTITPDHAAKFTPRVFIDGDAWIKPKGIPYAS 574
           +RVKW GIK +TP  A  +T   F+ GD WI    +PY +
Sbjct: 543 QRVKWPGIKKLTPQDALLYTGDRFLRGDTWIPQTQVPYTA 566

[Close]

BLAST of Lag0031495 vs. ExPASy Swiss-Prot
Match: Q42920 (Pectinesterase/pectinesterase inhibitor OS=Medicago sativa OX=3879 PE=2 SV=1)

HSP 1 Score: 466.5 bits (1199), Expect = 4.5e-130
Identity = 232/454 (51.10%), Postives = 314/454 (69.16%), Query Frame = 0


Query: 130 LQNCRELLEYAIDDLTQSFERIGTFEMSKLDQFVNDLKIWLSGALTYEQTCLDGFENTTG 189
           ++ C E+L+YA+D + +S   +  F+  KL ++  DLK+WL+G L+++QTCLDGF NTT 
Sbjct: 1   MEICNEVLDYAVDGIHKSVGTLDQFDFHKLSEYAFDLKVWLTGTLSHQQTCLDGFANTTT 60

Query: 190 DAGQKMQQFLKSAQEMTSNGLAIVTELSTIMGSLNQMPGATPGATGRRLL-DEEMPSWVS 249
            AG+ M + LK++ E++SN + ++  +S I+   +     +  +  RRLL D+ +PSWV+
Sbjct: 61  KAGETMTKVLKTSMELSSNAIDMMDAVSRILKGFD----TSQYSVSRRLLSDDGIPSWVN 120

Query: 250 EGKRRLMQAGPGGLKPDVVVAQDGSGKYKTISAALVDVPKKSNKTFVIYVKAGIYKEIVV 309
           +G RRL+ AG G ++P+ VVAQDGSG++KT++ AL  VP K+   FVI+VKAG+YKE V 
Sbjct: 121 DGHRRLL-AG-GNVQPNAVVAQDGSGQFKTLTDALKTVPPKNAVPFVIHVKAGVYKETVN 180

Query: 310 VPKDVTHLTMYGDGPTKTVVTGSLNFIDGIQTFKTATFSTIGTDFFAKDMGFVNSAGAAK 369
           V K++ ++T+ GDGPTKT  TGSLN+ DGI T+ TATF   G +F AKD+GF N+AG  K
Sbjct: 181 VAKEMNYVTVIGDGPTKTKFTGSLNYADGINTYNTATFGVNGANFMAKDIGFENTAGTGK 240

Query: 370 HQAVALRVQSDRAIFYNCQMDGYQDTLYAHAHRQFYRDCSISGTIDFVFGNAAAVFQNCK 429
           HQAVALRV +D+AIFYNCQMDG+QDTLY  + RQFYRDCSISGTIDFVFG    VFQNCK
Sbjct: 241 HQAVALRVTADQAIFYNCQMDGFQDTLYVQSQRQFYRDCSISGTIDFVFGERFGVFQNCK 300

Query: 430 LIVRKPMDNQQCIVTAQGRLNRREPTALVFQGCHFMGDPAYLPFKAVNKAYLGRPWKEYS 489
           L+ R P   QQC+VTA GR  +   +ALVFQ  HF G+PA L       +YLGRPWK+YS
Sbjct: 301 LVCRLPAKGQQCLVTAGGREKQNSASALVFQSSHFTGEPA-LTSVTPKVSYLGRPWKQYS 360

Query: 490 RTLIIGSQIDDLIQPEGWLPWGGNFGLNTLFYAEVQNKGPGADLSKRVKWRGIKTITPDH 549
           + +I+ S ID +  PEG++PW G+    T  Y E  NKGPGAD + RVKW G+K +T + 
Sbjct: 361 KVVIMDSTIDAIFVPEGYMPWMGSAFKETCTYYEYNNKGPGADTNLRVKWHGVKVLTSNV 420

Query: 550 AAKFTPRVFID------GDAWIKPKGIPYASGMM 577
           AA++ P  F +       D WI   G+PY+ G M
Sbjct: 421 AAEYYPGKFFEIVNATARDTWIVKSGVPYSLGPM 447

[Close]

BLAST of Lag0031495 vs. ExPASy TrEMBL
Match: A0A1S3C7B6 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103497833 PE=3 SV=1)

HSP 1 Score: 959.5 bits (2479), Expect = 6.2e-276
Identity = 471/578 (81.49%), Postives = 522/578 (90.31%), Query Frame = 0


Query: 2   SNAEGDKKKKNIAIVSVSSLILVAMVVAVTVGVNSSPYGDDHGEGHGDGHGDGGAEIKTS 61
           +NAEGDKKKK IAI+SVSSLILVAMVVAVTVG+N++PY ++HGE  GD       EI +S
Sbjct: 6   ANAEGDKKKKKIAIISVSSLILVAMVVAVTVGINNAPY-EEHGE--GDQGSKTTPEISSS 65

Query: 62  TKAIQSICRPTDYKETCEQSLMAEASNTTDPKELAKAGFKVAVEYINTAIRNSTTLKELA 121
           TKAIQ+IC+PTDYKETCE+SLMAEA NTTDPKEL K GFK+A   +N AIRNSTTL+EL+
Sbjct: 66  TKAIQAICQPTDYKETCEKSLMAEAGNTTDPKELVKVGFKIATRSLNEAIRNSTTLQELS 125

Query: 122 KEPRTNQALQNCRELLEYAIDDLTQSFERIGTFEMSKLDQFVNDLKIWLSGALTYEQTCL 181
           K+PRTNQALQNCRELLEYAIDDL +SFERIGTF+MSKLD+FV DLKIWLSGALTYEQTCL
Sbjct: 126 KDPRTNQALQNCRELLEYAIDDLNESFERIGTFQMSKLDEFVADLKIWLSGALTYEQTCL 185

Query: 182 DGFENTTGDAGQKMQQFLKSAQEMTSNGLAIVTELSTIMGSLNQMPGATPGATGRRLL-- 241
           DGFENTTGDAG +MQ+FLKSAQEMT+NGL IV ELSTI+GSL       PG TGRRLL  
Sbjct: 186 DGFENTTGDAGTRMQEFLKSAQEMTTNGLGIVNELSTILGSLQ-----IPGMTGRRLLED 245

Query: 242 -DEEMPSWVSEGKRRLMQAGPGGLKPDVVVAQDGSGKYKTISAALVDVPKKSNKTFVIYV 301
            D EMPSW+S+GKRRLMQAG  G+KPD++VAQDGSGKYKTI+AAL DVP KSNKTFVIYV
Sbjct: 246 DDHEMPSWISDGKRRLMQAGAAGMKPDLIVAQDGSGKYKTINAALADVPLKSNKTFVIYV 305

Query: 302 KAGIYKEIVVVPKDVTHLTMYGDGPTKTVVTGSLNFIDGIQTFKTATFSTIGTDFFAKDM 361
           KAGIYKEIVV+PK +THLTMYGDGPTKTVVTGSLNFIDGIQTFKTATFS IG +F+A+DM
Sbjct: 306 KAGIYKEIVVIPKHMTHLTMYGDGPTKTVVTGSLNFIDGIQTFKTATFSAIGANFYARDM 365

Query: 362 GFVNSAGAAKHQAVALRVQSDRAIFYNCQMDGYQDTLYAHAHRQFYRDCSISGTIDFVFG 421
           GF N+AG+AKHQAVALRVQSDR+IFYNCQ+DGYQDTLYAHAHRQFYRDC+ISGTIDFVFG
Sbjct: 366 GFENTAGSAKHQAVALRVQSDRSIFYNCQIDGYQDTLYAHAHRQFYRDCTISGTIDFVFG 425

Query: 422 NAAAVFQNCKLIVRKPMDNQQCIVTAQGRLNRREPTALVFQGCHFMGDPAYLPFKAVNKA 481
           NAA  FQNCKL+VRKPMDNQQCIVTA GR+NR+EPTAL+FQGCHFMGDPAYLPFK++NKA
Sbjct: 426 NAATNFQNCKLVVRKPMDNQQCIVTAHGRMNRKEPTALIFQGCHFMGDPAYLPFKSINKA 485

Query: 482 YLGRPWKEYSRTLIIGSQIDDLIQPEGWLPWGGNFGLNTLFYAEVQNKGPGADLSKRVKW 541
           YLGRPWK+YSRT+IIGSQIDD+IQPEGWLPW G+FGLNTLFYAEVQNKGPGA  +KRVKW
Sbjct: 486 YLGRPWKQYSRTIIIGSQIDDIIQPEGWLPWMGDFGLNTLFYAEVQNKGPGAVETKRVKW 545

Query: 542 RGIKTITPDHAAKFTPRVFIDGDAWIKPKGIPYASGMM 577
           RGIK ITP HAA FTPR FIDGDAWI  KGIPY+SGMM
Sbjct: 546 RGIKHITPQHAADFTPRRFIDGDAWIPAKGIPYSSGMM 575

[Close]

BLAST of Lag0031495 vs. ExPASy TrEMBL
Match: A0A0A0KD24 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_6G318700 PE=3 SV=1)

HSP 1 Score: 955.3 bits (2468), Expect = 1.2e-274
Identity = 472/579 (81.52%), Postives = 520/579 (89.81%), Query Frame = 0


Query: 2   SNAEGDKKKKNIAIVSVSSLILVAMVVAVTVGVNSSPYGDDHGEGHGDGHG-DGGAEIKT 61
           +NAEGDKKKK IAI+SVSSLILVAMVVAVTVGVN++P+G++HG+   D HG +   EI +
Sbjct: 6   ANAEGDKKKKKIAIISVSSLILVAMVVAVTVGVNNAPHGEEHGD---DNHGSETTPEITS 65

Query: 62  STKAIQSICRPTDYKETCEQSLMAEASNTTDPKELAKAGFKVAVEYINTAIRNSTTLKEL 121
           STKAIQ+IC+PTDYKETCE+SL AEA NTTDPKEL K GFK+A   +N AI+NSTTLKEL
Sbjct: 66  STKAIQAICQPTDYKETCEKSLEAEAGNTTDPKELVKVGFKIATRSLNEAIKNSTTLKEL 125

Query: 122 AKEPRTNQALQNCRELLEYAIDDLTQSFERIGTFEMSKLDQFVNDLKIWLSGALTYEQTC 181
           AK+PRTNQALQNCRELLEYAIDDL QSFERIGTF+MSKLD FV DLKIWLSGALTYEQTC
Sbjct: 126 AKDPRTNQALQNCRELLEYAIDDLNQSFERIGTFQMSKLDDFVADLKIWLSGALTYEQTC 185

Query: 182 LDGFENTTGDAGQKMQQFLKSAQEMTSNGLAIVTELSTIMGSLNQMPGATPGATGRRLL- 241
           LDGFENTTGDAG +MQ+FLKSAQ+MT+NGL IV ELSTI+GSL       PG +GRRLL 
Sbjct: 186 LDGFENTTGDAGVRMQEFLKSAQQMTTNGLGIVNELSTILGSLQ-----LPGMSGRRLLE 245

Query: 242 --DEEMPSWVSEGKRRLMQAGPGGLKPDVVVAQDGSGKYKTISAALVDVPKKSNKTFVIY 301
             D EMPSWVS+GKRRLMQAG   +KPD+VVAQDGSGKYKTI+AAL DVP KSNKTFVI+
Sbjct: 246 DDDREMPSWVSDGKRRLMQAGAAAMKPDLVVAQDGSGKYKTINAALADVPLKSNKTFVIH 305

Query: 302 VKAGIYKEIVVVPKDVTHLTMYGDGPTKTVVTGSLNFIDGIQTFKTATFSTIGTDFFAKD 361
           VKAGIYKEIVV+PK +THLTMYGDGPTKTVVTGSLNFIDGIQTFKTATFS IG +F+A+D
Sbjct: 306 VKAGIYKEIVVIPKHMTHLTMYGDGPTKTVVTGSLNFIDGIQTFKTATFSAIGANFYARD 365

Query: 362 MGFVNSAGAAKHQAVALRVQSDRAIFYNCQMDGYQDTLYAHAHRQFYRDCSISGTIDFVF 421
           MGF N+AGAAKHQAVALRVQSDR+IF+NCQ+DGYQDTLYAHAHRQFYRDC+ISGTIDFVF
Sbjct: 366 MGFENTAGAAKHQAVALRVQSDRSIFFNCQIDGYQDTLYAHAHRQFYRDCTISGTIDFVF 425

Query: 422 GNAAAVFQNCKLIVRKPMDNQQCIVTAQGRLNRREPTALVFQGCHFMGDPAYLPFKAVNK 481
           GNAA  FQNCKL+VRKP+DNQQCIVTA GRLNR+EPTAL+FQ CHFMGDPAYLPFKA+NK
Sbjct: 426 GNAATNFQNCKLVVRKPLDNQQCIVTAHGRLNRKEPTALIFQSCHFMGDPAYLPFKAINK 485

Query: 482 AYLGRPWKEYSRTLIIGSQIDDLIQPEGWLPWGGNFGLNTLFYAEVQNKGPGADLSKRVK 541
           AYLGRPWKEYSRT+IIGS IDD+IQPEGWLPW G+FGLNTLFYAEVQNKG GAD SKRVK
Sbjct: 486 AYLGRPWKEYSRTIIIGSTIDDIIQPEGWLPWMGDFGLNTLFYAEVQNKGAGADESKRVK 545

Query: 542 WRGIKTITPDHAAKFTPRVFIDGDAWIKPKGIPYASGMM 577
           WRGIK ITP HAA FTPR FIDGDAWI  KGIPY+SGMM
Sbjct: 546 WRGIKHITPQHAADFTPRRFIDGDAWIPAKGIPYSSGMM 576

[Close]

BLAST of Lag0031495 vs. ExPASy TrEMBL
Match: A0A6J1KZN0 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111499042 PE=3 SV=1)

HSP 1 Score: 927.9 bits (2397), Expect = 2.0e-266
Identity = 453/600 (75.50%), Postives = 518/600 (86.33%), Query Frame = 0


Query: 3   NAEGDKKKKNIAIVSVSSLILVAMVVAVTVGVNSSPYGD-----------DHGEGHGD-- 62
           +AEGDKKKK IA++SVSSLILVAMVVAVTVGVN++P+ D           DHG+  GD  
Sbjct: 6   DAEGDKKKKKIAVISVSSLILVAMVVAVTVGVNNAPHEDLDDGGGGGDLHDHGDDGGDVH 65

Query: 63  -------------GHGDGGAEIKTSTKAIQSICRPTDYKETCEQSLMAEASNTTDPKELA 122
                         HGD G +I +STKAI++IC+PTDYKETCE+SLM EA NTTD KEL 
Sbjct: 66  DHGDYGGGGGDAHDHGDDGEKISSSTKAIKTICQPTDYKETCEESLMVEAGNTTDLKELV 125

Query: 123 KAGFKVAVEYINTAIRNSTTLKELAKEPRTNQALQNCRELLEYAIDDLTQSFERIGTFEM 182
           KAGFK+AVE +N AIRNSTTL+ELAK+PR NQALQNCRELLEYAIDDL  S+ RIGTF +
Sbjct: 126 KAGFKIAVEKLNAAIRNSTTLQELAKDPRANQALQNCRELLEYAIDDLHDSYHRIGTFHV 185

Query: 183 SKLDQFVNDLKIWLSGALTYEQTCLDGFENTTGDAGQKMQQFLKSAQEMTSNGLAIVTEL 242
           SK ++FVNDLKIWLS ALTYEQTCLDGFENTTGD+G++M++FLKSAQEMT+NGL IV+EL
Sbjct: 186 SKFEEFVNDLKIWLSAALTYEQTCLDGFENTTGDSGERMKEFLKSAQEMTTNGLGIVSEL 245

Query: 243 STIMGSLNQMPGATPGATGRRLLDEEMPSWVSEGKRRLMQAGPGGLKPDVVVAQDGSGKY 302
           S I+G+L++     P ATGRRLLD +MP WVS+GKRRLMQ   G +KPD+VVAQDGSGKY
Sbjct: 246 SNILGTLHE-----PAATGRRLLDTDMPLWVSDGKRRLMQLETGAIKPDLVVAQDGSGKY 305

Query: 303 KTISAALVDVPKKSNKTFVIYVKAGIYKEIVVVPKDVTHLTMYGDGPTKTVVTGSLNFID 362
           KTI+ ALVDVP KSNKTFVIYVKAGIYKEIVV+PK +THLTMYGDGPTK+VVTG+LNF+D
Sbjct: 306 KTINEALVDVPMKSNKTFVIYVKAGIYKEIVVIPKHMTHLTMYGDGPTKSVVTGNLNFVD 365

Query: 363 GIQTFKTATFSTIGTDFFAKDMGFVNSAGAAKHQAVALRVQSDRAIFYNCQMDGYQDTLY 422
           GIQTFKTATF+ IG +FF +DMGFVNSAG  KHQAVALR+QSDRAI YNCQ+DG+QDTLY
Sbjct: 366 GIQTFKTATFAAIGANFFVRDMGFVNSAGPTKHQAVALRIQSDRAIIYNCQIDGFQDTLY 425

Query: 423 AHAHRQFYRDCSISGTIDFVFGNAAAVFQNCKLIVRKPMDNQQCIVTAQGRLNRREPTAL 482
           AHA+RQFYRDC+ISGTIDFVFGN+AA+FQNCKL+VRKP+DNQQCIVTA GRLNRREPTAL
Sbjct: 426 AHANRQFYRDCTISGTIDFVFGNSAAIFQNCKLVVRKPLDNQQCIVTAHGRLNRREPTAL 485

Query: 483 VFQGCHFMGDPAYLPFKAVNKAYLGRPWKEYSRTLIIGSQIDDLIQPEGWLPWGGNFGLN 542
           +FQGCHFMGDPAYLPFK VNKAYLGRPWK+YSRT+I+GSQIDDLIQPEGWLPW G+FGLN
Sbjct: 486 IFQGCHFMGDPAYLPFKKVNKAYLGRPWKQYSRTIIMGSQIDDLIQPEGWLPWMGDFGLN 545

Query: 543 TLFYAEVQNKGPGADLSKRVKWRGIKTITPDHAAKFTPRVFIDGDAWIKPKGIPYASGMM 577
           TLFYAEVQN+GPGAD S RVKWRGI+ ITP HAA +T R FIDGDAWI  +G+PY+SGMM
Sbjct: 546 TLFYAEVQNRGPGADQSHRVKWRGIRHITPQHAASYTARRFIDGDAWIPVRGVPYSSGMM 600

[Close]

BLAST of Lag0031495 vs. ExPASy TrEMBL
Match: A0A6J1H4H8 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111460411 PE=3 SV=1)

HSP 1 Score: 923.7 bits (2386), Expect = 3.8e-265
Identity = 449/579 (77.55%), Postives = 512/579 (88.43%), Query Frame = 0


Query: 3   NAEGDKKKKNIAIVSVSSLILVAMVVAVTVGVNSSPYGD-DHGEGHGDG----HGDGGAE 62
           +AE DKKKK IA++SVSSLILVAMVVAVTVGVN++P  D D G G G G    HGD   +
Sbjct: 6   DAESDKKKKKIAVISVSSLILVAMVVAVTVGVNNAPQEDLDDGGGGGGGDAHDHGDDAEK 65

Query: 63  IKTSTKAIQSICRPTDYKETCEQSLMAEASNTTDPKELAKAGFKVAVEYINTAIRNSTTL 122
           I +STKAI++IC+PTDYKETCE+SLM EA NTTDPKEL KAGFK+AVE +N AIRNSTTL
Sbjct: 66  ISSSTKAIKTICQPTDYKETCEESLMVEAGNTTDPKELVKAGFKIAVEKLNAAIRNSTTL 125

Query: 123 KELAKEPRTNQALQNCRELLEYAIDDLTQSFERIGTFEMSKLDQFVNDLKIWLSGALTYE 182
           +ELA++PR NQALQNCRELLEYAIDDL  S+ RIGTF +SK ++FVNDLKIWLS ALTYE
Sbjct: 126 QELAQDPRANQALQNCRELLEYAIDDLHDSYHRIGTFHVSKFEEFVNDLKIWLSAALTYE 185

Query: 183 QTCLDGFENTTGDAGQKMQQFLKSAQEMTSNGLAIVTELSTIMGSLNQMPGATPGATGRR 242
           QTCLDGFENTTGD+G++M++FLKSAQEMT+NGL IV+ELS I+G+L+      P ATGRR
Sbjct: 186 QTCLDGFENTTGDSGERMKEFLKSAQEMTTNGLGIVSELSKILGTLD-----APAATGRR 245

Query: 243 LLDEEMPSWVSEGKRRLMQAGPGGLKPDVVVAQDGSGKYKTISAALVDVPKKSNKTFVIY 302
           LLD ++P WVS+GKRRLMQ G G +KPD+VVAQDGSGKYKTI+ ALV VP KSNKTFVIY
Sbjct: 246 LLDTDIPLWVSDGKRRLMQVGTGAIKPDLVVAQDGSGKYKTINEALVHVPMKSNKTFVIY 305

Query: 303 VKAGIYKEIVVVPKDVTHLTMYGDGPTKTVVTGSLNFIDGIQTFKTATFSTIGTDFFAKD 362
           VKAGIYKEIVV+PK +THLTMYGDGPTK+VVTG+LNF+DGIQTFKTATF+ IG +FF +D
Sbjct: 306 VKAGIYKEIVVIPKHMTHLTMYGDGPTKSVVTGNLNFVDGIQTFKTATFAAIGANFFVRD 365

Query: 363 MGFVNSAGAAKHQAVALRVQSDRAIFYNCQMDGYQDTLYAHAHRQFYRDCSISGTIDFVF 422
           MGFVN+AG  KHQAVALRVQSDRAI YNCQ+DGYQDTLYAHA+RQFYRDC ISGTIDFVF
Sbjct: 366 MGFVNTAGPTKHQAVALRVQSDRAIIYNCQIDGYQDTLYAHANRQFYRDCIISGTIDFVF 425

Query: 423 GNAAAVFQNCKLIVRKPMDNQQCIVTAQGRLNRREPTALVFQGCHFMGDPAYLPFKAVNK 482
           GN+AA+FQNCKL+VRKP+DNQQCIVTA GRLNRREPTAL+FQGCHFMGDP+YLPFK VNK
Sbjct: 426 GNSAAIFQNCKLVVRKPLDNQQCIVTAHGRLNRREPTALIFQGCHFMGDPSYLPFKKVNK 485

Query: 483 AYLGRPWKEYSRTLIIGSQIDDLIQPEGWLPWGGNFGLNTLFYAEVQNKGPGADLSKRVK 542
           AYLGRPWK+YSRT+I+GSQIDDLIQPEGWLPW G+F LNTLFYAEVQN+GPGAD S RVK
Sbjct: 486 AYLGRPWKQYSRTIIMGSQIDDLIQPEGWLPWMGDFALNTLFYAEVQNRGPGADQSHRVK 545

Query: 543 WRGIKTITPDHAAKFTPRVFIDGDAWIKPKGIPYASGMM 577
           WRGI+ ITP HAA +T R FIDGDAWI  +G+PY+SGMM
Sbjct: 546 WRGIRHITPQHAAAYTARRFIDGDAWIPVRGVPYSSGMM 579

[Close]

BLAST of Lag0031495 vs. ExPASy TrEMBL
Match: A0A6J1KJ31 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111494433 PE=3 SV=1)

HSP 1 Score: 896.0 bits (2314), Expect = 8.4e-257
Identity = 437/575 (76.00%), Postives = 497/575 (86.43%), Query Frame = 0


Query: 3   NAEGDKKKKNIAIVSVSSLILVAMVVAVTVGVNSSPYGDDHGEGHGDGHGDGGAEIKTST 62
           +AEGD KKK +A V VSSL LVAMVVAVTVGVN+S +    GEG GDGHGDG  EI +ST
Sbjct: 6   DAEGDNKKKKMAFVGVSSLFLVAMVVAVTVGVNNSSH---KGEGLGDGHGDGAGEISSST 65

Query: 63  KAIQSICRPTDYKETCEQSLMAEASNTTDPKELAKAGFKVAVEYINTAIRNSTTLKELAK 122
           KAIQ+ICRPTDYKETCE+SLM+E SNTTDP+EL K GFKV ++ +N+AIRNSTTL+ LAK
Sbjct: 66  KAIQTICRPTDYKETCEKSLMSEGSNTTDPRELVKIGFKVCMDQLNSAIRNSTTLQALAK 125

Query: 123 EPRTNQALQNCRELLEYAIDDLTQSFERIGTFEMSKLDQFVNDLKIWLSGALTYEQTCLD 182
           EPRTNQALQNCRELLEYAIDD+T SF RIGTF+M+KLD+FV DL IWLSGALTYEQTCLD
Sbjct: 126 EPRTNQALQNCRELLEYAIDDITDSFNRIGTFQMAKLDKFVEDLSIWLSGALTYEQTCLD 185

Query: 183 GFENTTGDAGQKMQQFLKSAQEMTSNGLAIVTELSTIMGSLNQMPGATPGATGRRLL-DE 242
           GFENTTGDAG++M+ FLKSAQEMTSNGLAIVTEL  ++GSL           GRRLL  +
Sbjct: 186 GFENTTGDAGERMKMFLKSAQEMTSNGLAIVTELKNMLGSLQ------GSKAGRRLLAGD 245

Query: 243 EMPSWVSEGKRRLMQAGPGGLKPDVVVAQDGSGKYKTISAALVDVPKKSNKTFVIYVKAG 302
           E+PSWVS  KRRLMQ     + P+VVVAQDGSGKYKTI+ ALVDVPKKSN TFVIYVKAG
Sbjct: 246 EVPSWVSSEKRRLMQLPRSAITPNVVVAQDGSGKYKTINEALVDVPKKSNITFVIYVKAG 305

Query: 303 IYKEIVVVPKDVTHLTMYGDGPTKTVVTGSLNFIDGIQTFKTATFSTIGTDFFAKDMGFV 362
           IYKE V++PK +TH+TMYGDGPTKT+V+G+LNF+DGIQTFKTATF+ +G +FF +DMGF 
Sbjct: 306 IYKETVLIPKALTHVTMYGDGPTKTIVSGALNFVDGIQTFKTATFAVVGANFFCRDMGFE 365

Query: 363 NSAGAAKHQAVALRVQSDRAIFYNCQMDGYQDTLYAHAHRQFYRDCSISGTIDFVFGNAA 422
           N+AG+AKHQAVALRVQSDR+IF+NCQMDGYQDTLYA  HRQFYRDC +SGTIDFVFGN+A
Sbjct: 366 NTAGSAKHQAVALRVQSDRSIFFNCQMDGYQDTLYAQTHRQFYRDCKVSGTIDFVFGNSA 425

Query: 423 AVFQNCKLIVRKPMDNQQCIVTAQGRLNRREPTALVFQGCHFMGDPAYLPFKAVNKAYLG 482
           AVFQNC L+VRKP+ NQQCIVTAQGRL  REPT  VFQ CH MGDPAYLPFKAVNKAYLG
Sbjct: 426 AVFQNCNLVVRKPLANQQCIVTAQGRLEAREPTGFVFQKCHLMGDPAYLPFKAVNKAYLG 485

Query: 483 RPWKEYSRTLIIGSQIDDLIQPEGWLPWGGNFGLNTLFYAEVQNKGPGADLSKRVKWRGI 542
           RPWK+YSRT+I+GS+IDDLIQPEGWLPW G+FGLN+LFYAE QNKGPGADL+ RVKW+GI
Sbjct: 486 RPWKQYSRTIIMGSEIDDLIQPEGWLPWMGDFGLNSLFYAEFQNKGPGADLTNRVKWQGI 545

Query: 543 KTITPDHAAKFTPRVFIDGDAWIKPKGIPYASGMM 577
           KT+T   AA FTP+VFIDGDAWI   G+PY+ GMM
Sbjct: 546 KTLTAQQAADFTPKVFIDGDAWILNTGVPYSPGMM 571

[Close]

BLAST of Lag0031495 vs. TAIR 10
Match: AT3G05610.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 648.3 bits (1671), Expect = 5.9e-186
Identity = 306/571 (53.59%), Postives = 417/571 (73.03%), Query Frame = 0


Query: 8   KKKKNIAIVSVSSLILVAMVVAVTVGVNSSPYGDDHGEGHGDGHGDGGAEIKTSTKAIQS 67
           K+K+   ++++SS++L++MVVAVTVGV+ + +         DG   G AE+  S KA++ 
Sbjct: 10  KRKRRYIVITISSVLLISMVVAVTVGVSLNKH---------DGDSKGKAEVNASVKAVKD 69

Query: 68  ICRPTDYKETCEQSLMAEASNTTDPKELAKAGFKVAVEYINTAIRNSTTLKELAKEPRTN 127
           +C PTDY++TCE +L+    NTTDP EL K  F V ++ I  A + S T+ EL K+ RT 
Sbjct: 70  VCAPTDYRKTCEDTLIKNGKNTTDPMELVKTAFNVTMKQITDAAKKSQTIMELQKDSRTR 129

Query: 128 QALQNCRELLEYAIDDLTQSFERIGTFEMSKLDQFVNDLKIWLSGALTYEQTCLDGFENT 187
            AL  C+EL++YA+D+L+ SFE +G FE   LD+ + +L+IWLS A+++E+TCL+GF+ T
Sbjct: 130 MALDQCKELMDYALDELSNSFEELGKFEFHLLDEALINLRIWLSAAISHEETCLEGFQGT 189

Query: 188 TGDAGQKMQQFLKSAQEMTSNGLAIVTELSTIMGSLNQMPGATPGATGRRLLDEEMPSWV 247
            G+AG+ M++ LK+A E+T NGLAI++E+S  +G +       PG   RRLL E  PSWV
Sbjct: 190 QGNAGETMKKALKTAIELTHNGLAIISEMSNFVGQMQ-----IPGLNSRRLLAEGFPSWV 249

Query: 248 SEGKRRLMQAGP--GGLKPDVVVAQDGSGKYKTISAALVDVPKKSNKTFVIYVKAGIYKE 307
            +  R+L+QA      +KPD+VVAQDGSG+YKTI+ AL  VPKK N TFV+++KAG+YKE
Sbjct: 250 DQRGRKLLQAAAAYSDVKPDIVVAQDGSGQYKTINEALQFVPKKRNTTFVVHIKAGLYKE 309

Query: 308 IVVVPKDVTHLTMYGDGPTKTVVTGSLNFIDGIQTFKTATFSTIGTDFFAKDMGFVNSAG 367
            V V K ++HL   GDGP KT+++G+ N+ DGI T++TAT + +G  F AK++GF N+AG
Sbjct: 310 YVQVNKTMSHLVFIGDGPDKTIISGNKNYKDGITTYRTATVAIVGNYFIAKNIGFENTAG 369

Query: 368 AAKHQAVALRVQSDRAIFYNCQMDGYQDTLYAHAHRQFYRDCSISGTIDFVFGNAAAVFQ 427
           A KHQAVA+RVQSD +IF+NC+ DGYQDTLY H+HRQF+RDC+ISGTIDF+FG+AAAVFQ
Sbjct: 370 AIKHQAVAVRVQSDESIFFNCRFDGYQDTLYTHSHRQFFRDCTISGTIDFLFGDAAAVFQ 429

Query: 428 NCKLIVRKPMDNQQCIVTAQGRLNRREPTALVFQGCHFMGDPAYLPFKAVNKAYLGRPWK 487
           NC L+VRKP+ NQ C +TA GR + RE T  VFQGC   G+P YL  K  +KAYLGRPWK
Sbjct: 430 NCTLLVRKPLPNQACPITAHGRKDPRESTGFVFQGCTIAGEPDYLAVKETSKAYLGRPWK 489

Query: 488 EYSRTLIIGSQIDDLIQPEGWLPWGGNFGLNTLFYAEVQNKGPGADLSKRVKWRGIKTIT 547
           EYSRT+I+ + I D +QP+GW PW G+FGL TLFY+EVQN GPG+ L+ RV W GIKT++
Sbjct: 490 EYSRTIIMNTFIPDFVQPQGWQPWLGDFGLKTLFYSEVQNTGPGSALANRVTWAGIKTLS 549

Query: 548 PDHAAKFTPRVFIDGDAWIKPKGIPYASGMM 577
            +   KFTP  +I GD WI  KG+PY +G++
Sbjct: 550 EEDILKFTPAQYIQGDDWIPGKGVPYTTGLL 566

[Close]

BLAST of Lag0031495 vs. TAIR 10
Match: AT5G27870.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 639.8 bits (1649), Expect = 2.1e-183
Identity = 310/571 (54.29%), Postives = 409/571 (71.63%), Query Frame = 0


Query: 5   EGDKKKKNIAIVSVSSLILVAMVVAVTVGVNSSPYGDDHGEGHGDGHGDGGAEIKTSTKA 64
           E  K+KK   I+S+SS++L++MVVAVT+GV+ +           D  GD   EI TS KA
Sbjct: 8   EDAKRKKRYVIISISSVLLISMVVAVTIGVSVN---------KSDNAGD--EEITTSVKA 67

Query: 65  IQSICRPTDYKETCEQSLMAEASNTTDPKELAKAGFKVAVEYINTAIRNSTTLKELAKEP 124
           I+ +C PTDYKETCE +L  +A +T+DP EL K  F   ++ I+   + S T+ EL K+P
Sbjct: 68  IKDVCAPTDYKETCEDTLRKDAKDTSDPLELVKTAFNATMKQISDVAKKSQTMIELQKDP 127

Query: 125 RTNQALQNCRELLEYAIDDLTQSFERIGTFEMSKLDQFVNDLKIWLSGALTYEQTCLDGF 184
           R   AL  C+EL++YAI +L++SFE +G FE  K+D+ +  L+IWLS  +++EQTCLDGF
Sbjct: 128 RAKMALDQCKELMDYAIGELSKSFEELGKFEFHKVDEALVKLRIWLSATISHEQTCLDGF 187

Query: 185 ENTTGDAGQKMQQFLKSAQEMTSNGLAIVTELSTIMGSLNQMPGATPGATGRRLLDEEMP 244
           + T G+AG+ +++ LK+A ++T NGLA+VTE+S  +G +       P    RRLL +E P
Sbjct: 188 QGTQGNAGETIKKALKTAVQLTHNGLAMVTEMSNYLGQMQ-----IPEMNSRRLLSQEFP 247

Query: 245 SWVSEGKRRLMQAGPGGLKPDVVVAQDGSGKYKTISAALVDVPKKSNKTFVIYVKAGIYK 304
           SW+    RRL+ A    +KPD+VVAQDGSG+YKTI+ AL  VPKK N TFV+++K GIYK
Sbjct: 248 SWMDARARRLLNAPMSEVKPDIVVAQDGSGQYKTINEALNFVPKKKNTTFVVHIKEGIYK 307

Query: 305 EIVVVPKDVTHLTMYGDGPTKTVVTGSLNFIDGIQTFKTATFSTIGTDFFAKDMGFVNSA 364
           E V V + +THL   GDGP KTV++GS ++ DGI T+KTAT + +G  F AK++ F N+A
Sbjct: 308 EYVQVNRSMTHLVFIGDGPDKTVISGSKSYKDGITTYKTATVAIVGDHFIAKNIAFENTA 367

Query: 365 GAAKHQAVALRVQSDRAIFYNCQMDGYQDTLYAHAHRQFYRDCSISGTIDFVFGNAAAVF 424
           GA KHQAVA+RV +D +IFYNC+ DGYQDTLYAH+HRQFYRDC+ISGTIDF+FG+AAAVF
Sbjct: 368 GAIKHQAVAIRVLADESIFYNCKFDGYQDTLYAHSHRQFYRDCTISGTIDFLFGDAAAVF 427

Query: 425 QNCKLIVRKPMDNQQCIVTAQGRLNRREPTALVFQGCHFMGDPAYLPFKAVNKAYLGRPW 484
           QNC L+VRKP+ NQ C +TA GR + RE T  V QGC  +G+P YL  K  +K YLGRPW
Sbjct: 428 QNCTLLVRKPLLNQACPITAHGRKDPRESTGFVLQGCTIVGEPDYLAVKEQSKTYLGRPW 487

Query: 485 KEYSRTLIIGSQIDDLIQPEGWLPWGGNFGLNTLFYAEVQNKGPGADLSKRVKWRGIKTI 544
           KEYSRT+I+ + I D + PEGW PW G FGLNTLFY+EVQN GPGA ++KRV W GIK +
Sbjct: 488 KEYSRTIIMNTFIPDFVPPEGWQPWLGEFGLNTLFYSEVQNTGPGAAITKRVTWPGIKKL 547

Query: 545 TPDHAAKFTPRVFIDGDAWIKPKGIPYASGM 576
           + +   KFTP  +I GDAWI  KG+PY  G+
Sbjct: 548 SDEEILKFTPAQYIQGDAWIPGKGVPYILGL 562

[Close]

BLAST of Lag0031495 vs. TAIR 10
Match: AT5G49180.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 582.0 bits (1499), Expect = 5.2e-166
Identity = 287/576 (49.83%), Postives = 397/576 (68.92%), Query Frame = 0


Query: 5   EGDKKKKNIAIVSVSSLILVAMVVAVTVGVNSSPYGDDHGEGHGDGHGDG--GAEIKTST 64
           +G+ KKK   I  V + +LV MVVA  VG+ +S             H +     +IKT+T
Sbjct: 4   DGELKKKKCIIAGVITALLVLMVVA--VGITTS---------RNTSHSEKIVPVQIKTAT 63

Query: 65  KAIQSICRPTDYKETCEQSLMAEASNTTDPKELAKAGFKVAVEYINTAIRNSTT--LKEL 124
            A++++C PTDYKETC  SLM  + ++T P +L K GF V +  I  +I+ ++     + 
Sbjct: 64  TAVEAVCAPTDYKETCVNSLMKASPDSTQPLDLIKLGFNVTIRSIEDSIKKASVELTAKA 123

Query: 125 AKEPRTNQALQNCRELLEYAIDDLTQSFERIGTFEMSKLDQFVNDLKIWLSGALTYEQTC 184
           A +  T  AL+ C +L+  A DDL +  +    F + +++ FV DL++WLSG++ Y+QTC
Sbjct: 124 ANDKDTKGALELCEKLMNDATDDLKKCLDNFDGFSIPQIEDFVEDLRVWLSGSIAYQQTC 183

Query: 185 LDGFENTTGDAGQKMQQFLKSAQEMTSNGLAIVTELSTIMGSLNQMPGATP--GATGRRL 244
           +D FE T     Q MQ+  K+++E+TSNGLA++T +S ++G  N + G T   G   R+L
Sbjct: 184 MDTFEETNSKLSQDMQKIFKTSRELTSNGLAMITNISNLLGEFN-VTGVTGDLGKYARKL 243

Query: 245 LDEE--MPSWVSEGKRRLMQAGPGGLKPDVVVAQDGSGKYKTISAALVDVPKKSNKTFVI 304
           L  E  +PSWV    RRLM A  GG+K +VVVA DGSG+YKTI+ AL  VPK + K FVI
Sbjct: 244 LSAEDGIPSWVGPNTRRLM-ATKGGVKANVVVAHDGSGQYKTINEALNAVPKANQKPFVI 303

Query: 305 YVKAGIYKEIVVVPKDVTHLTMYGDGPTKTVVTGSLNFIDG-IQTFKTATFSTIGTDFFA 364
           Y+K G+Y E V V K +TH+T  GDGPTKT +TGSLN+  G ++T+ TAT +  G +F A
Sbjct: 304 YIKQGVYNEKVDVTKKMTHVTFIGDGPTKTKITGSLNYYIGKVKTYLTATVAINGDNFTA 363

Query: 365 KDMGFVNSAGAAKHQAVALRVQSDRAIFYNCQMDGYQDTLYAHAHRQFYRDCSISGTIDF 424
           K++GF N+AG   HQAVALRV +D A+FYNCQ+DGYQDTLY H+HRQF+RDC++SGT+DF
Sbjct: 364 KNIGFENTAGPEGHQAVALRVSADLAVFYNCQIDGYQDTLYVHSHRQFFRDCTVSGTVDF 423

Query: 425 VFGNAAAVFQNCKLIVRKPMDNQQCIVTAQGRLNRREPTALVFQGCHFMGDPAYLPFKAV 484
           +FG+   V QNC ++VRKPM +Q C++TAQGR ++RE T LV Q CH  G+PAY+P K++
Sbjct: 424 IFGDGIVVLQNCNIVVRKPMKSQSCMITAQGRSDKRESTGLVLQNCHITGEPAYIPVKSI 483

Query: 485 NKAYLGRPWKEYSRTLIIGSQIDDLIQPEGWLPWGGNFGLNTLFYAEVQNKGPGADLSKR 544
           NKAYLGRPWKE+SRT+I+G+ IDD+I P GWLPW G+F LNTL+YAE +N GPG++ ++R
Sbjct: 484 NKAYLGRPWKEFSRTIIMGTTIDDVIDPAGWLPWNGDFALNTLYYAEYENNGPGSNQAQR 543

Query: 545 VKWRGIKTITPDHAAKFTPRVFIDGDAWIKPKGIPY 572
           VKW GIK ++P  A +FTP  F+ G+ WI P  +PY
Sbjct: 544 VKWPGIKKLSPKQALRFTPARFLRGNLWIPPNRVPY 566

[Close]

BLAST of Lag0031495 vs. TAIR 10
Match: AT3G06830.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 541.6 bits (1394), Expect = 7.8e-154
Identity = 275/580 (47.41%), Postives = 388/580 (66.90%), Query Frame = 0


Query: 4   AEGDKKKKNIAIVSVSSLILVAMVVAVTVGVNSSPYGDDHGEGHGDGHGDGGAEIKTSTK 63
           ++GDKKKK I   SVS  +++ +V    V    SP  D++              I+ +TK
Sbjct: 3   SDGDKKKKFIVAGSVSGFLVIMVVSVAVVTSKHSPKDDEN-------------HIRKTTK 62

Query: 64  AIQSICRPTDYKETCEQSLMAEASNTTDPKELAKAGFKVAVEYINTAI-RNSTTLKELA- 123
           A+Q++C PTD+K+TC  SLM  + ++ DP +L K GFKV ++ IN ++ + S  +K  A 
Sbjct: 63  AVQAVCAPTDFKDTCVNSLMGASPDSDDPVDLIKLGFKVTIKSINESLEKASGDIKAKAD 122

Query: 124 KEPRTNQALQNCRELLEYAIDDLTQSFERIGTFEMSKLDQFVNDLKIWLSGALTYEQTCL 183
           K P    A + C +L+  AIDDL +  +    F + +++ FV DL++WLSG++ ++QTC+
Sbjct: 123 KNPEAKGAFELCEKLMIDAIDDLKKCMDH--GFSVDQIEVFVEDLRVWLSGSIAFQQTCM 182

Query: 184 DGFENTTGDAGQKMQQFLKSAQEMTSNGLAIVTELSTIMGSLNQMPGATPGAT--GRRLL 243
           D F     +  Q M +  K+++E++SN LA+VT +ST++ + N + G T       R+LL
Sbjct: 183 DSFGEIKSNLMQDMLKIFKTSRELSSNSLAMVTRISTLIPNSN-LTGLTGALAKYARKLL 242

Query: 244 --DEEMPSWVSEGKRRLMQA---GPGGLKPDVVVAQDGSGKYKTISAALVDVPKKSNKTF 303
             ++ +P+WV    RRLM A   GPG +K + VVAQDG+G++KTI+ AL  VPK +   F
Sbjct: 243 STEDSIPTWVGPEARRLMAAQGGGPGPVKANAVVAQDGTGQFKTITDALNAVPKGNKVPF 302

Query: 304 VIYVKAGIYKEIVVVPKDVTHLTMYGDGPTKTVVTGSLNF-IDGIQTFKTATFSTIGTDF 363
           +I++K GIYKE V V K + H+T  GDGP KT++TGSLNF I  ++TF TAT +  G  F
Sbjct: 303 IIHIKEGIYKEKVTVTKKMPHVTFIGDGPNKTLITGSLNFGIGKVKTFLTATITIEGDHF 362

Query: 364 FAKDMGFVNSAGAAKHQAVALRVQSDRAIFYNCQMDGYQDTLYAHAHRQFYRDCSISGTI 423
            AK++G  N+AG    QAVALRV +D A+F++CQ+DG+QDTLY H+HRQFYRDC++SGT+
Sbjct: 363 TAKNIGIENTAGPEGGQAVALRVSADYAVFHSCQIDGHQDTLYVHSHRQFYRDCTVSGTV 422

Query: 424 DFVFGNAAAVFQNCKLIVRKPMDNQQCIVTAQGRLNRREPTALVFQGCHFMGDPAYLPFK 483
           DF+FG+A  + QNCK++VRKP   Q C+VTAQGR N RE T LV  GCH  GDPAY+P K
Sbjct: 423 DFIFGDAKCILQNCKIVVRKPNKGQTCMVTAQGRSNVRESTGLVLHGCHITGDPAYIPMK 482

Query: 484 AVNKAYLGRPWKEYSRTLIIGSQIDDLIQPEGWLPWGGNFGLNTLFYAEVQNKGPGADLS 543
           +VNKAYLGRPWKE+SRT+I+ + IDD+I P GWLPW G+F L TL+YAE  N GPG++ +
Sbjct: 483 SVNKAYLGRPWKEFSRTIIMKTTIDDVIDPAGWLPWSGDFALKTLYYAEHMNTGPGSNQA 542

Query: 544 KRVKWRGIKTITPDHAAKFTPRVFIDGDAWIKPKGIPYAS 574
           +RVKW GIK +TP  A  +T   F+ GD WI    +PY +
Sbjct: 543 QRVKWPGIKKLTPQDALLYTGDRFLRGDTWIPQTQVPYTA 566

[Close]

BLAST of Lag0031495 vs. TAIR 10
Match: AT4G15980.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 460.3 bits (1183), Expect = 2.3e-129
Identity = 275/703 (39.12%), Postives = 375/703 (53.34%), Query Frame = 0


Query: 15  IVSVSSLILVAMVVAVTVGVNSSPYGDDHGEGHGDGHGDGGAEIKTSTKAIQSICRPTDY 74
           ++ V++LI+ AMV+ V     +SP          + H +    I  +T +I  IC  TDY
Sbjct: 6   LLGVTALIM-AMVICVEANDGNSP-----SRKMEEVHRESKLMITKTTVSI--ICASTDY 65

Query: 75  KETCEQSLMAEASNTTDPKELAKAGFKVAVEYINTAI-RNSTTLKELA-KEPRTNQALQN 134
           K+ C  SL    S   DP+ L ++ F +A+  I + I R    LK  A  +  T +AL  
Sbjct: 66  KQDCTTSLATVRS--PDPRNLIRSAFDLAIISIRSGIDRGMIDLKSRADADMHTREALNT 125

Query: 135 CRELLEYAIDDLTQSFERIGTFEMSKLDQFVNDLKIWLSGALTYEQTCLDGFENTTGDAG 194
           CREL++ AIDDL ++ ++   F  ++L  FV DL +WLSG++TY+QTC+DGFE    +A 
Sbjct: 126 CRELMDDAIDDLRKTRDKFRGFLFTRLSDFVEDLCVWLSGSITYQQTCIDGFEGIDSEAA 185

Query: 195 QKMQQFLKSAQEMTSNGLAIVTEL------------------------------------ 254
             M++ ++  Q +TSNGLAI   L                                    
Sbjct: 186 VMMERVMRKGQHLTSNGLAIAANLDKLLKAFRIPFPFLRSRRGRLGILGSGSSRDESVGS 245

Query: 255 ---------------STIMGSLNQMPGA----------------------------TP-- 314
                          ST+  S NQ   +                            TP  
Sbjct: 246 SQDSPPNEDSSDDSPSTVDSSENQPVDSSSENQSSDSSNNRPLDSSKNQQMESSEDTPKK 305

Query: 315 -GATGRRLLDE------------------------------------------------- 374
              +G + LD+                                                 
Sbjct: 306 SAFSGNQPLDDSSDKLPQKSTSSENQPLDSSENPPQKSTSSENRPLDPLRKLNPLNKLDS 365

Query: 375 ----------EMPSWVSEGKRRLMQAGP--GGLKPDVVVAQDGSGKYKTISAALVDVPKK 434
                     E P WV+   RRL+   P   G+K +VVVA+DGSGK KTI+ AL  VP K
Sbjct: 366 LKDRHLSEEGEFPPWVTPHSRRLLARRPRNNGIKANVVVAKDGSGKCKTIAQALAMVPMK 425

Query: 435 SNKTFVIYVKAGIYKEIVVVPKDVTHLTMYGDGPTKTVVTGSLNFI-DGIQTFKTATFST 494
           + K FVI++K G+YKE V V K + H+   GDGPTKTV+TG + F+ D + T++TA+ + 
Sbjct: 426 NTKKFVIHIKEGVYKEKVEVTKKMLHVMFVGDGPTKTVITGDIAFLPDQVGTYRTASVAV 485

Query: 495 IGTDFFAKDMGFVNSAGAAKHQAVALRVQSDRAIFYNCQMDGYQDTLYAHAHRQFYRDCS 554
            G  F AKD+GF N+AGAA+HQAVALRV +D A+F+NC M+GYQDTLY H HRQFYR+C 
Sbjct: 486 NGDYFMAKDIGFENTAGAARHQAVALRVSADFAVFFNCHMNGYQDTLYVHTHRQFYRNCR 545

Query: 555 ISGTIDFVFGNAAAVFQNCKLIVRKPMDNQQCIVTAQGRLNRREPTALVFQGCHFMGDPA 572
           +SGTIDFVFG+A AVFQNC+ ++R+PM++QQCIVTAQGR +RRE T +V       GD +
Sbjct: 546 VSGTIDFVFGDAKAVFQNCEFVIRRPMEHQQCIVTAQGRKDRRETTGIVIHNSRITGDAS 605

The following BLAST results are available for this feature:

BLAST of Lag0031495 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008458409.1	1.3e-275	81.49	PREDICTED: probable pectinesterase/pectinesterase inhibitor 21 [Cucumis melo]	[more]
XP_004150503.1	2.4e-274	81.52	probable pectinesterase/pectinesterase inhibitor 21 [Cucumis sativus] >KGN47423....	[more]
XP_038875843.1	1.5e-271	80.10	probable pectinesterase/pectinesterase inhibitor 21 [Benincasa hispida]	[more]
XP_023549205.1	9.8e-268	78.24	probable pectinesterase/pectinesterase inhibitor 21 [Cucurbita pepo subsp. pepo]	[more]
XP_023006255.1	4.1e-266	75.50	probable pectinesterase/pectinesterase inhibitor 21 [Cucurbita maxima]	[more]

BLAST of Lag0031495 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8GX86	8.3e-185	53.59	Probable pectinesterase/pectinesterase inhibitor 21 OS=Arabidopsis thaliana OX=3...	[more]
Q3E8Z8	3.0e-182	54.29	Putative pectinesterase/pectinesterase inhibitor 28 OS=Arabidopsis thaliana OX=3...	[more]
Q9FJ21	7.3e-165	49.83	Probable pectinesterase/pectinesterase inhibitor 58 OS=Arabidopsis thaliana OX=3...	[more]
Q8GXA1	1.1e-152	47.41	Probable pectinesterase/pectinesterase inhibitor 23 OS=Arabidopsis thaliana OX=3...	[more]
Q42920	4.5e-130	51.10	Pectinesterase/pectinesterase inhibitor OS=Medicago sativa OX=3879 PE=2 SV=1	[more]

BLAST of Lag0031495 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3C7B6	6.2e-276	81.49	Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103497833 PE=3 SV=1	[more]
A0A0A0KD24	1.2e-274	81.52	Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_6G318700 PE=3 SV=1	[more]
A0A6J1KZN0	2.0e-266	75.50	Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111499042 PE=3 SV=1	[more]
A0A6J1H4H8	3.8e-265	77.55	Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111460411 PE=3 SV=1	[more]
A0A6J1KJ31	8.4e-257	76.00	Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111494433 PE=3 SV=1	[more]

BLAST of Lag0031495 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G05610.1	5.9e-186	53.59	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT5G27870.1	2.1e-183	54.29	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT5G49180.1	5.2e-166	49.83	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT3G06830.1	7.8e-154	47.41	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT4G15980.1	2.3e-129	39.12	Plant invertase/pectin methylesterase inhibitor superfamily	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 60..212 e-value: 2.7E-39 score: 146.5
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 64..212 e-value: 4.3E-29 score: 101.8
IPR006501	Pectinesterase inhibitor domain	TIGRFAM	TIGR01614	TIGR01614	coord: 60..216 e-value: 1.2E-25 score: 87.9
IPR000070	Pectinesterase, catalytic	PFAM	PF01095	Pectinesterase	coord: 265..561 e-value: 6.3E-137 score: 455.2
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 260..575 e-value: 1.4E-116 score: 391.3
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	GENE3D	1.20.140.40	Invertase/pectin methylesterase inhibitor family protein	coord: 59..217 e-value: 1.6E-35 score: 124.4
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	SUPERFAMILY	101148	Plant invertase/pectin methylesterase inhibitor	coord: 63..216
None	No IPR available	PANTHER	PTHR31707:SF337	PECTINESTERASE	coord: 13..575
None	No IPR available	PANTHER	PTHR31707	PECTINESTERASE	coord: 13..575
None	No IPR available	CDD	cd15798	PMEI-like_3	coord: 67..218 e-value: 2.19362E-48 score: 163.763
IPR033131	Pectinesterase, Asp active site	PROSITE	PS00503	PECTINESTERASE_2	coord: 409..418
IPR018040	Pectinesterase, Tyr active site	PROSITE	PS00800	PECTINESTERASE_1	coord: 290..309
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 263..573

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0031495.1	Lag0031495.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042545	cell wall modification
biological_process	GO:0043086	negative regulation of catalytic activity
biological_process	GO:0045490	pectin catabolic process
cellular_component	GO:0005576	extracellular region
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0045330	aspartyl esterase activity
molecular_function	GO:0030599	pectinesterase activity
molecular_function	GO:0046910	pectinesterase inhibitor activity
molecular_function	GO:0004857	enzyme inhibitor activity