Lag0031456 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0031456
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	Pectinesterase
Location	chr11: 8734455 .. 8735027 (+)
RNA-Seq Expression	Lag0031456
Synteny	Lag0031456

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCGCTTCCGCTGCGTGGGGACTTTTATTCCCTTCACCTCCTTCCTCTCCCTCTTCTCTTCCAAACCCATATCTTTGAATCCCCAATTTCACCAAACAACTCTCTCCCTCTGCAATGGCACTCTGTACCCACACATTTGTTTCTCAAAACTCACCTCTTTCCCCGAATTGGCCTCCCTCTCGCCGGAAAAGCTCGCCAGCAGCGCCCTCAACTTCACCGACGTCGAGGTCCACGACGCCTACGCCAATTGCACCAAGCTCAAGACCCTCCTCTATGGCAGCCTCAATCCCACAGACCGCCGCGCTCTCGATGACTGTCTCGAGCTTCTCGACGACAGCATTGTTGAGCTCGAAACTTCGATTTTTGATCTAGCACCGTCGCAATGCCCCACCAGCCACTCCCACGATCTCTTGAGTATTGATTTGGAAATGTTGAAGAAAATCTCTGGAGTTAATCGGGCTACTGCGGCGGAGGAGGTGTTGCCGGAGTATGGGGCGATGAAGGGTGGGTTTCTGTTACGGATCAGGAATTTCACTATCGTTGGTGAAGCGGTCGCCGCCACTCCGAATTAA

mRNA sequence

ATGCGCTTCCGCTGCGTGGGGACTTTTATTCCCTTCACCTCCTTCCTCTCCCTCTTCTCTTCCAAACCCATATCTTTGAATCCCCAATTTCACCAAACAACTCTCTCCCTCTGCAATGGCACTCTGTACCCACACATTTGTTTCTCAAAACTCACCTCTTTCCCCGAATTGGCCTCCCTCTCGCCGGAAAAGCTCGCCAGCAGCGCCCTCAACTTCACCGACGTCGAGGTCCACGACGCCTACGCCAATTGCACCAAGCTCAAGACCCTCCTCTATGGCAGCCTCAATCCCACAGACCGCCGCGCTCTCGATGACTGTCTCGAGCTTCTCGACGACAGCATTGTTGAGCTCGAAACTTCGATTTTTGATCTAGCACCGTCGCAATGCCCCACCAGCCACTCCCACGATCTCTTGAGTATTGATTTGGAAATGTTGAAGAAAATCTCTGGAGTTAATCGGGCTACTGCGGCGGAGGAGGTGTTGCCGGAGTATGGGGCGATGAAGGGTGGGTTTCTGTTACGGATCAGGAATTTCACTATCGTTGGTGAAGCGGTCGCCGCCACTCCGAATTAA

Coding sequence (CDS)

ATGCGCTTCCGCTGCGTGGGGACTTTTATTCCCTTCACCTCCTTCCTCTCCCTCTTCTCTTCCAAACCCATATCTTTGAATCCCCAATTTCACCAAACAACTCTCTCCCTCTGCAATGGCACTCTGTACCCACACATTTGTTTCTCAAAACTCACCTCTTTCCCCGAATTGGCCTCCCTCTCGCCGGAAAAGCTCGCCAGCAGCGCCCTCAACTTCACCGACGTCGAGGTCCACGACGCCTACGCCAATTGCACCAAGCTCAAGACCCTCCTCTATGGCAGCCTCAATCCCACAGACCGCCGCGCTCTCGATGACTGTCTCGAGCTTCTCGACGACAGCATTGTTGAGCTCGAAACTTCGATTTTTGATCTAGCACCGTCGCAATGCCCCACCAGCCACTCCCACGATCTCTTGAGTATTGATTTGGAAATGTTGAAGAAAATCTCTGGAGTTAATCGGGCTACTGCGGCGGAGGAGGTGTTGCCGGAGTATGGGGCGATGAAGGGTGGGTTTCTGTTACGGATCAGGAATTTCACTATCGTTGGTGAAGCGGTCGCCGCCACTCCGAATTAA

Protein sequence

MRFRCVGTFIPFTSFLSLFSSKPISLNPQFHQTTLSLCNGTLYPHICFSKLTSFPELASLSPEKLASSALNFTDVEVHDAYANCTKLKTLLYGSLNPTDRRALDDCLELLDDSIVELETSIFDLAPSQCPTSHSHDLLSIDLEMLKKISGVNRATAAEEVLPEYGAMKGGFLLRIRNFTIVGEAVAATPN

Homology

[Close]

BLAST of Lag0031456 vs. NCBI nr
Match: KAG7014347.1 (putative pectinesterase/pectinesterase inhibitor 40 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 239.2 bits (609), Expect = 2.9e-59
Identity = 136/250 (54.40%), Postives = 151/250 (60.40%), Query Frame = 0


Query: 6   VGTFIPFTSFLSLFSSKPISLNPQFHQTTLSLCNGTLYPHICFSKLTSFPELASLSPEKL 65
           +     F S L L S KPIS NPQ HQT  SLCNGTL+P++CFSKL+SFP+LASLSPEKL
Sbjct: 18  ISAIFTFFSVLFLCSFKPISSNPQTHQTARSLCNGTLFPNLCFSKLSSFPQLASLSPEKL 77

Query: 66  ASSALNFTDVEVHDAYANCTKLKTLLYGSLNPTDRRALDDCLELLDDSIVELETSIFDLA 125
           A SALNFT+ EVH AYANC+KLKTL YG LNPTDRRALDDCLELLDDSI EL+TSI+DL+
Sbjct: 78  AGSALNFTNDEVHGAYANCSKLKTLFYGGLNPTDRRALDDCLELLDDSIAELQTSIYDLS 137

Query: 126 PSQCPTSHSHDL---------------------------------------LSIDLEMLK 185
           PSQ    HSHDL                                       LSIDL MLK
Sbjct: 138 PSQSAARHSHDLLTLVSAAMTNHRTCLDGFYNSSGNVRKRVELYLGKIEQHLSIDLAMLK 197

Query: 186 KISGVNRATAAEEVLPEYGAMKGGFLLRIR--------------------------NFTI 191
           KI GV RA  AEEVLPEYGA++GGF + +                           NFT 
Sbjct: 198 KIPGVKRAATAEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNETKFNLVVAKDGSGNFTT 257

[Close]

BLAST of Lag0031456 vs. NCBI nr
Match: XP_022991224.1 (pectinesterase-like [Cucurbita maxima])

HSP 1 Score: 237.3 bits (604), Expect = 1.1e-58
Identity = 135/242 (55.79%), Postives = 149/242 (61.57%), Query Frame = 0


Query: 14  SFLSLFSSKPISLNPQFHQTTLSLCNGTLYPHICFSKLTSFPELASLSPEKLASSALNFT 73
           S L LFS KPIS NPQ HQT  SLCNGTL+P++CFSKL+SFP+LASLSPEKLA SALNFT
Sbjct: 26  SVLFLFSFKPISSNPQTHQTARSLCNGTLFPNLCFSKLSSFPQLASLSPEKLAGSALNFT 85

Query: 74  DVEVHDAYANCTKLKTLLYGSLNPTDRRALDDCLELLDDSIVELETSIFDLAPSQCPTSH 133
           + EVH AYANC+KLKTL YG LNPTDRRALDDCLELLD SI EL+TSI+DL+PSQ    H
Sbjct: 86  NDEVHGAYANCSKLKTLFYGGLNPTDRRALDDCLELLDGSIAELQTSIYDLSPSQSAARH 145

Query: 134 SHDL---------------------------------------LSIDLEMLKKISGVNRA 191
           SHDL                                       LSIDL MLKKI GV RA
Sbjct: 146 SHDLLTLVSAAMTNHRTCLDGFYNSSGTVRKRVELYLGKIEQHLSIDLAMLKKIPGVKRA 205

[Close]

BLAST of Lag0031456 vs. NCBI nr
Match: KAG6575805.1 (putative pectinesterase/pectinesterase inhibitor 40, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 235.7 bits (600), Expect = 3.2e-58
Identity = 134/250 (53.60%), Postives = 150/250 (60.00%), Query Frame = 0


Query: 6   VGTFIPFTSFLSLFSSKPISLNPQFHQTTLSLCNGTLYPHICFSKLTSFPELASLSPEKL 65
           +     F S + L S KPIS NPQ HQT  SLCNGTL+P++CFSKL+SFP+LASLSPEKL
Sbjct: 18  ISAIFTFFSVIFLCSFKPISSNPQTHQTARSLCNGTLFPNLCFSKLSSFPQLASLSPEKL 77

Query: 66  ASSALNFTDVEVHDAYANCTKLKTLLYGSLNPTDRRALDDCLELLDDSIVELETSIFDLA 125
           A SALNFT+ EVH AYANC+KLKTL YG LNPTDRRALDDCLELLD SI EL+TSI+DL+
Sbjct: 78  AGSALNFTNNEVHGAYANCSKLKTLFYGGLNPTDRRALDDCLELLDGSIAELQTSIYDLS 137

Query: 126 PSQCPTSHSHDL---------------------------------------LSIDLEMLK 185
           PSQ    HSHDL                                       LSIDL MLK
Sbjct: 138 PSQSAARHSHDLLTLVSAAMTNHRTCLDGFYNSSGTVRKRVELYLGKIEQHLSIDLAMLK 197

Query: 186 KISGVNRATAAEEVLPEYGAMKGGFLLRIR--------------------------NFTI 191
           KI GV RA  AEEVLPEYGA++GGF + +                           NFT 
Sbjct: 198 KIPGVKRAATAEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNETKFNLVVAKDGSGNFTT 257

[Close]

BLAST of Lag0031456 vs. NCBI nr
Match: XP_023548978.1 (pectinesterase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 234.6 bits (597), Expect = 7.2e-58
Identity = 134/250 (53.60%), Postives = 150/250 (60.00%), Query Frame = 0


Query: 6   VGTFIPFTSFLSLFSSKPISLNPQFHQTTLSLCNGTLYPHICFSKLTSFPELASLSPEKL 65
           +     F S L L S KPIS NPQ HQT  SLCNGTL+P++CFSKL+SFP+LASLSPEKL
Sbjct: 18  ISAIFTFFSVLILCSFKPISSNPQTHQTARSLCNGTLFPNLCFSKLSSFPQLASLSPEKL 77

Query: 66  ASSALNFTDVEVHDAYANCTKLKTLLYGSLNPTDRRALDDCLELLDDSIVELETSIFDLA 125
           A SALNFT+ EVH AYANC+KLKTL YG L+PTDRRALDDCLELLD SI EL+TSI+DL+
Sbjct: 78  AGSALNFTNDEVHGAYANCSKLKTLFYGGLSPTDRRALDDCLELLDGSIAELQTSIYDLS 137

Query: 126 PSQCPTSHSHDL---------------------------------------LSIDLEMLK 185
           PSQ    HSHDL                                       LSIDL MLK
Sbjct: 138 PSQSAARHSHDLLTLVSAAMTNHRTCLDGFYNSSGTVRKRVELYLGKIEQHLSIDLAMLK 197

Query: 186 KISGVNRATAAEEVLPEYGAMKGGFLLRIR--------------------------NFTI 191
           KI GV RA  AEEVLPEYGA++GGF + +                           NFT 
Sbjct: 198 KIPGVKRAATAEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNETKFNLVVAKDGSGNFTT 257

[Close]

BLAST of Lag0031456 vs. NCBI nr
Match: XP_022953706.1 (pectinesterase-like [Cucurbita moschata])

HSP 1 Score: 234.2 bits (596), Expect = 9.4e-58
Identity = 134/250 (53.60%), Postives = 149/250 (59.60%), Query Frame = 0


Query: 6   VGTFIPFTSFLSLFSSKPISLNPQFHQTTLSLCNGTLYPHICFSKLTSFPELASLSPEKL 65
           +     F S L L S KPIS NPQ HQT  SLCNGTL+P++CFSKL+SFP+LASLSPEKL
Sbjct: 18  ISAIFTFFSVLFLCSFKPISSNPQTHQTARSLCNGTLFPNLCFSKLSSFPQLASLSPEKL 77

Query: 66  ASSALNFTDVEVHDAYANCTKLKTLLYGSLNPTDRRALDDCLELLDDSIVELETSIFDLA 125
           A SALNFT+ EVH AYANC+KLKTL YG LNPTDRRALDDCLELLD SI EL+TSI+DL+
Sbjct: 78  AGSALNFTNDEVHGAYANCSKLKTLFYGGLNPTDRRALDDCLELLDGSIAELQTSIYDLS 137

Query: 126 PSQCPTSHSHDL---------------------------------------LSIDLEMLK 185
           PSQ    HSHDL                                       LSIDL MLK
Sbjct: 138 PSQSAARHSHDLLTLVSAAMTNHRTCLDGFYNSSGTVRKRVELYLGKIEQHLSIDLAMLK 197

Query: 186 KISGVNRATAAEEVLPEYGAMKGGFLLRIR--------------------------NFTI 191
           KI GV RA  AEEVLPEYGA++GGF + +                           NFT 
Sbjct: 198 KIPGVKRAATAEEVLPEYGAVRGGFPMWVSVKDRRLLQASVNETKFNLVVAKDGSGNFTT 257

[Close]

BLAST of Lag0031456 vs. ExPASy Swiss-Prot
Match: O04886 (Pectinesterase 1 OS=Citrus sinensis OX=2711 GN=PECS-1.1 PE=2 SV=1)

HSP 1 Score: 58.5 bits (140), Expect = 9.3e-08
Identity = 40/134 (29.85%), Postives = 63/134 (47.01%), Query Frame = 0


Query: 27  NPQFHQTTLSLCNGTLYPHICFSKLTSFPELAS--LSPEKLASSALNFTDVEVHDAYANC 86
           N   H    S C+ T YP +CFS + + PE +    S + +   +LN T   V   Y   
Sbjct: 62  NEPHHAILKSSCSSTRYPDLCFSAIAAVPEASKKVTSQKDVIEMSLNITTTAVEHNYFGI 121

Query: 87  TKLKTLLYGSLNPTDRRALDDCLELLDDSIVELETSIFDLAPSQCPTSHSHDLLSIDLEM 146
            KL  L   +L   ++ AL DCLE +D+++ EL  ++ DL   + P   S    + DL+ 
Sbjct: 122 QKL--LKRTNLTKREKVALHDCLETIDETLDELHKAVEDL--EEYPNKKSLSQHADDLKT 181

Query: 147 LKKISGVNRATAAE 159
           L   +  N+ T  +
Sbjct: 182 LMSAAMTNQGTCLD 191

[Close]

BLAST of Lag0031456 vs. ExPASy Swiss-Prot
Match: Q42534 (Pectinesterase 2 OS=Arabidopsis thaliana OX=3702 GN=PME2 PE=2 SV=2)

HSP 1 Score: 58.5 bits (140), Expect = 9.3e-08
Identity = 42/139 (30.22%), Postives = 67/139 (48.20%), Query Frame = 0


Query: 20  SSKPISLNPQFHQTTLSLCNGTLYPHICFSKLTSFPELASLSPEKLASSALNFTDVEVHD 79
           + K  +L+   H    S+C+ TLYP +CFS + +       S +++  ++LN T   V  
Sbjct: 48  NQKITTLSSTSHAILKSVCSSTLYPELCFSAVAATGGKELTSQKEVIEASLNLTTKAVKH 107

Query: 80  AYANCTKLKTLLYGSLNPTDRRALDDCLELLDDSIVELETSIFDLAPSQCPTSHSHDLLS 139
            Y    KL     G L P +  AL DCLE +D+++ EL  ++ DL   Q P   S    +
Sbjct: 108 NYFAVKKLIAKRKG-LTPREVTALHDCLETIDETLDELHVAVEDL--HQYPKQKSLRKHA 167

Query: 140 IDLEMLKKISGVNRATAAE 159
            DL+ L   +  N+ T  +
Sbjct: 168 DDLKTLISSAITNQGTCLD 183

[Close]

BLAST of Lag0031456 vs. ExPASy Swiss-Prot
Match: P83948 (Pectinesterase 3 OS=Citrus sinensis OX=2711 PE=1 SV=1)

HSP 1 Score: 58.5 bits (140), Expect = 9.3e-08
Identity = 40/134 (29.85%), Postives = 63/134 (47.01%), Query Frame = 0


Query: 27  NPQFHQTTLSLCNGTLYPHICFSKLTSFPELAS--LSPEKLASSALNFTDVEVHDAYANC 86
           N   H    S C+ T YP +CFS + + PE +    S + +   +LN T   V   Y   
Sbjct: 62  NEPHHAILKSSCSSTRYPDLCFSAIAAVPEASKKVTSQKDVIEMSLNITTTAVEHNYFGI 121

Query: 87  TKLKTLLYGSLNPTDRRALDDCLELLDDSIVELETSIFDLAPSQCPTSHSHDLLSIDLEM 146
            KL  L   +L   ++ AL DCLE +D+++ EL  ++ DL   + P   S    + DL+ 
Sbjct: 122 QKL--LKRTNLTKREKVALHDCLETIDETLDELHKAVEDL--EEYPNKKSLSQHADDLKT 181

Query: 147 LKKISGVNRATAAE 159
           L   +  N+ T  +
Sbjct: 182 LMSAAMTNQGTCLD 191

[Close]

BLAST of Lag0031456 vs. ExPASy Swiss-Prot
Match: O49006 (Pectinesterase/pectinesterase inhibitor 3 OS=Arabidopsis thaliana OX=3702 GN=PME3 PE=1 SV=2)

HSP 1 Score: 54.7 bits (130), Expect = 1.3e-06
Identity = 31/100 (31.00%), Postives = 51/100 (51.00%), Query Frame = 0


Query: 25  SLNPQFHQTTLSLCNGTLYPHICFSKLTSFPELASLSPEKLASSALNFTDVEVHDAYANC 84
           +L+P  H    S C+ T YP +C S + +   +   S + +  +++N T   V   Y   
Sbjct: 50  TLSPSSHAVLRSSCSSTRYPELCISAVVTAGGVELTSQKDVIEASVNLTITAVEHNYFTV 109

Query: 85  TKLKTLLYGSLNPTDRRALDDCLELLDDSIVELETSIFDL 125
            KL     G L P ++ AL DCLE +D+++ EL  ++ DL
Sbjct: 110 KKLIKKRKG-LTPREKTALHDCLETIDETLDELHETVEDL 148

[Close]

BLAST of Lag0031456 vs. ExPASy Swiss-Prot
Match: Q9SI74 (Pectinesterase inhibitor 10 OS=Arabidopsis thaliana OX=3702 GN=PMEI10 PE=2 SV=1)

HSP 1 Score: 50.8 bits (120), Expect = 1.9e-05
Identity = 41/136 (30.15%), Postives = 66/136 (48.53%), Query Frame = 0


Query: 20  SSKPI-SLNPQFHQTTLS----------LCNGTLYPHICFSKLTSFPELASLSPEKLASS 79
           SS P+ SL+P    +T S           CN TLY  IC++ L+ +      +P+KLA  
Sbjct: 123 SSSPLSSLSPSSSSSTYSNQTNLDYIKTSCNITLYKTICYNSLSPYASTIRSNPQKLAVI 182

Query: 80  ALNFTDVEVHDAYANCTKLKTLLY-GSLNPTDRRALDDCLELLDDSIVELETSIFDLAPS 139
           ALN T   +  A +    +K + + G L   +  A+ DC+E + DS+  L+ SI +L   
Sbjct: 183 ALNLT---LSSAKSASKFVKNISHGGGLTRLEVVAVADCVEEIGDSVTSLQDSIRELDSI 242

Query: 140 QCPTSHSHDLLSIDLE 144
               S   +++  D+E
Sbjct: 243 NYKDSAKFEMVMSDVE 255

The following BLAST results are available for this feature:

BLAST of Lag0031456 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7014347.1	2.9e-59	54.40	putative pectinesterase/pectinesterase inhibitor 40 [Cucurbita argyrosperma subs...	[more]
XP_022991224.1	1.1e-58	55.79	pectinesterase-like [Cucurbita maxima]	[more]
KAG6575805.1	3.2e-58	53.60	putative pectinesterase/pectinesterase inhibitor 40, partial [Cucurbita argyrosp...	[more]
XP_023548978.1	7.2e-58	53.60	pectinesterase-like [Cucurbita pepo subsp. pepo]	[more]
XP_022953706.1	9.4e-58	53.60	pectinesterase-like [Cucurbita moschata]	[more]

BLAST of Lag0031456 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O04886	9.3e-08	29.85	Pectinesterase 1 OS=Citrus sinensis OX=2711 GN=PECS-1.1 PE=2 SV=1	[more]
Q42534	9.3e-08	30.22	Pectinesterase 2 OS=Arabidopsis thaliana OX=3702 GN=PME2 PE=2 SV=2	[more]
P83948	9.3e-08	29.85	Pectinesterase 3 OS=Citrus sinensis OX=2711 PE=1 SV=1	[more]
O49006	1.3e-06	31.00	Pectinesterase/pectinesterase inhibitor 3 OS=Arabidopsis thaliana OX=3702 GN=PME...	[more]
Q9SI74	1.9e-05	30.15	Pectinesterase inhibitor 10 OS=Arabidopsis thaliana OX=3702 GN=PMEI10 PE=2 SV=1	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01

Position :

Zoom :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 36..125 e-value: 1.3E-12 score: 48.3
IPR006501	Pectinesterase inhibitor domain	TIGRFAM	TIGR01614	TIGR01614	coord: 8..125 e-value: 4.5E-14 score: 50.2
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	GENE3D	1.20.140.40	Invertase/pectin methylesterase inhibitor family protein	coord: 35..151 e-value: 1.8E-15 score: 59.2
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	SUPERFAMILY	101148	Plant invertase/pectin methylesterase inhibitor	coord: 36..127
None	No IPR available	PANTHER	PTHR31707	PECTINESTERASE	coord: 29..159
None	No IPR available	PANTHER	PTHR31707:SF257	PECTINESTERASE	coord: 29..159

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0031456.1	Lag0031456.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0004857	enzyme inhibitor activity