Lag0031442 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0031442 vs. NCBI nr
Match: KAA0034843.1 (retrotransposon protein [Cucumis melo var. makuwa])

HSP 1 Score: 359.4 bits (921), Expect = 3.0e-95
Identity = 196/354 (55.37%), Postives = 224/354 (63.28%), Query Frame = 0

Query: 1   MDRRCFAILCTLLKTVAGLSSTEVVDVEEMVAMFLHIVAHDVKNRVVRTQFARSGESVSR 60
           MDRRCFAILC LL+T+AGL+STEVVDVEEMVAMFLHI+AHDVKNRV++ +F RSGE++SR
Sbjct: 67  MDRRCFAILCHLLRTIAGLTSTEVVDVEEMVAMFLHILAHDVKNRVIQREFMRSGETISR 126

Query: 61  HFNTVLHVVLRLHDVLLKKPEPVTASCTDPRWKWFQNCLGALDGTYIKVNVVLS------ 120
           HFN VL  V+RLHD LLKKP+PV   CTD RW+WF+NCLGALDGTYIKVNV  S      
Sbjct: 127 HFNMVLLAVIRLHDELLKKPQPVPNECTDQRWRWFENCLGALDGTYIKVNVPASDRARYR 186

Query: 121 ----------------------IARGWEGSAADSRVLRDAISRPNGLTVPKGYYYLCDAG 180
                                 +  GWEGSAADSR+LRDA+SRPN L VPKGYYYL DAG
Sbjct: 187 TRKGEVATNVLGVYDTKGDFVYVLTGWEGSAADSRILRDALSRPNRLKVPKGYYYLVDAG 246

Query: 181 YPNAEGFLAPYRGERYHLSEWR--------------------------------GRWAIL 240
           YPNAEGFLAPYRG+RYHL EWR                                GRWAIL
Sbjct: 247 YPNAEGFLAPYRGQRYHLQEWRGPKNAPSTSKEFFNMKHSSARNVIERAFGVLKGRWAIL 306

Query: 241 RGKSYYPVRIQGGPSQHAAYFTIL---LIERWVTVKFLMSWMRWILLLLQLMNASSSRAP 292
           RGKSY+PV +Q     H      L   LI R +T   +   +        +   SSSR P
Sbjct: 307 RGKSYHPVEVQ----CHTILACCLLHNLINREMTNFDIEDNI--------VSMTSSSRLP 366

BLAST of Lag0031442 vs. NCBI nr
Match: KAA0036474.1 (retrotransposon protein [Cucumis melo var. makuwa])

HSP 1 Score: 345.5 bits (885), Expect = 4.5e-91
Identity = 183/352 (51.99%), Postives = 221/352 (62.78%), Query Frame = 0

Query: 1   MDRRCFAILCTLLKTVAGLSSTEVVDVEEMVAMFLHIVAHDVKNRVVRTQFARSGESVSR 60
           MDRR FAILC LL+ VAGLSSTE+VDVEEMVAMFLHI AHDVKNRV++ +F RSGE+VSR
Sbjct: 40  MDRRTFAILCHLLRNVAGLSSTEIVDVEEMVAMFLHIFAHDVKNRVIQREFVRSGETVSR 99

Query: 61  HFNTVLHVVLRLHDVLLKKPEPVTASCTDPRWKWFQNCLGALDGTYIKVNV--------- 120
           HFN VL  VLRL++ L+K+P PVT++C D RWK F+NCLGALDGTYIKVNV         
Sbjct: 100 HFNIVLLAVLRLYEELIKRPVPVTSNCNDQRWKCFENCLGALDGTYIKVNVPAGDRPTFR 159

Query: 121 -------------------VLSIARGWEGSAADSRVLRDAISRPNGLTVPKGYYYLCDAG 180
                               + +  GW+GSAADSR+LRDAISR NGL VPKGYYYLCDAG
Sbjct: 160 TRKGEIATNVLGVCDTKGDFVYVLAGWKGSAADSRILRDAISRENGLQVPKGYYYLCDAG 219

Query: 181 YPNAEGFLAPYRGERYHLSEWR--------------------------------GRWAIL 240
           YPNAEGFLAPYRG+RYHL EWR                                GRWAIL
Sbjct: 220 YPNAEGFLAPYRGQRYHLQEWRGAANAPTNAKEYFNMKHSSARNVIERAFGVLKGRWAIL 279

Query: 241 RGKS--YYPVRIQGGPSQHAAYFTILLIERWVTVKFLMSWMRW-----ILLLLQLMNASS 286
           RGKS   Y   ++      + Y T    E    ++    W +W       + +    ++S
Sbjct: 280 RGKSEMTYCDDVEDEDEGDSTYATTTASEDIQYIETTNEWSQWRDDLAASMFIDWHMSTS 339

BLAST of Lag0031442 vs. NCBI nr
Match: ADN34114.1 (retrotransposon protein [Cucumis melo subsp. melo])

HSP 1 Score: 343.6 bits (880), Expect = 1.7e-90
Identity = 186/362 (51.38%), Postives = 217/362 (59.94%), Query Frame = 0

Query: 15  TVAGLSSTEVVDVEEMVAMFLHIVAHDVKNRVVRTQFARSGESVSRHFNTVLHVVLRLHD 74
           T+AGL+STEVVDVEEMVAMFLHI+AHDVK+RV++ +F RSGE++SRHFN VL  V+RLH+
Sbjct: 57  TIAGLTSTEVVDVEEMVAMFLHILAHDVKSRVIKREFMRSGETISRHFNMVLLAVIRLHE 116

Query: 75  VLLKKPEPVTASCTDPRWKWFQNCLGALDGTYIKVNVVLS-------------------- 134
            LLKKP+PV   CTD RW+WF+NCLGALDGTYIKVNV  S                    
Sbjct: 117 ELLKKPQPVPNECTDQRWRWFENCLGALDGTYIKVNVPASDRARYRTRKGEVATNVLGVC 176

Query: 135 --------IARGWEGSAADSRVLRDAISRPNGLTVPKGYYYLCDAGYPNAEGFLAPYRGE 194
                   +  GWEGSAADSR+LRDA+SRPN L VPKGYYYL D GYPNAEGFLAPYRG+
Sbjct: 177 DTKGDFVYVLAGWEGSAADSRILRDALSRPNRLKVPKGYYYLVDVGYPNAEGFLAPYRGQ 236

Query: 195 RYHLSEWR--------------------------------GRWAILRGKSYYPVRIQGGP 254
           RYHL EWR                                GRWAILRGKSYYPV +Q   
Sbjct: 237 RYHLQEWRGPENAPSTSKEFFNMKHYSARNVIERAFGVLKGRWAILRGKSYYPVEVQ-CR 296

Query: 255 SQHAAYFTILLIERWVT-------------------------VKFLMSWMRWILLLLQLM 292
           +  A      LI R +T                         ++    W +W   L + +
Sbjct: 297 TILACCLLHNLINREMTNFDIEDNIDEVDSTHATTAADDIHYIETSNEWSQWRDNLAEEI 356

BLAST of Lag0031442 vs. NCBI nr
Match: TYK02751.1 (putative nuclease HARBI1 [Cucumis melo var. makuwa])

HSP 1 Score: 325.9 bits (834), Expect = 3.7e-85
Identity = 173/313 (55.27%), Postives = 196/313 (62.62%), Query Frame = 0

Query: 1   MDRRCFAILCTLLKTVAGLSSTEVVDVEEMVAMFLHIVAHDVKNRVVRTQFARSGESVSR 60
           MDRRCF ILC LL+TVAGL+S EVVDVEEMVAMFLHIVAHDVKNRV++ +F RSGE++SR
Sbjct: 1   MDRRCFTILCHLLRTVAGLTSIEVVDVEEMVAMFLHIVAHDVKNRVIQREFMRSGETISR 60

Query: 61  HFNTVLHVVLRLHDVLLKKPEPVTASCTDPRWKWFQNCLGALDGTYIKVNVVLS------ 120
           HFN VL VV+RLHD LLKKP+PV   CTD RW+WF+NCLGALDGTYIKVNV  S      
Sbjct: 61  HFNMVLLVVIRLHDKLLKKPQPVNNDCTDQRWRWFENCLGALDGTYIKVNVPASDRARYR 120

Query: 121 ----------------------IARGWEGSAADSRVLRDAISRPNGLTVPKGYYYLCDAG 180
                                 +  GWEGSAADSR+L DAISRPNGL VPKGYYYL DAG
Sbjct: 121 THKGEVATNVLGLCDTKGDFVYVLAGWEGSAADSRILHDAISRPNGLKVPKGYYYLVDAG 180

Query: 181 YPNAEGFLAPYRGERYHLSEWRGRWAILRGKSYYPVRIQGGPSQHAAYFTILLIERWVTV 240
           YPN +GFLA YRG+RYHL EWRG              ++  PS    +F           
Sbjct: 181 YPNVDGFLAAYRGQRYHLQEWRG--------------VENAPSTSKEFFN---------- 240

Query: 241 KFLMSWMRWILLLLQLMNASSSRAPKHIWTKNEDAKLVESLVSLVHAGGWRSDNGTFKAG 286
                           M  SS+R      TK E+A LVE LV LV+ GGWRSDNGTF  G
Sbjct: 241 ----------------MKHSSARN-----TKEEEAGLVECLVELVNGGGWRSDNGTFHPG 268

BLAST of Lag0031442 vs. NCBI nr
Match: KAA0065306.1 (retrotransposon protein [Cucumis melo var. makuwa])

HSP 1 Score: 312.0 bits (798), Expect = 5.5e-81
Identity = 177/366 (48.36%), Postives = 208/366 (56.83%), Query Frame = 0

Query: 1   MDRRCFAILCTLLKTVAGLSSTEVVDVEEMVAMFLHIVAHDVKNRVVRTQFARSGESVSR 60
           MDRRCF ILCT+L+T  GL +T+ VDVEEM+A+FLHIVAHDVKNRV R  FARSGE+VSR
Sbjct: 1   MDRRCFTILCTMLRTKGGLEATQYVDVEEMIAIFLHIVAHDVKNRVTRRHFARSGETVSR 60

Query: 61  HFNTVLHVVLRLHDVLLKKPEPVTASCTDPRWKWFQNCLGALDGTYIKVNVVLS------ 120
           HFN     VLRLH++LLK+P+PVT SC+  +W+WFQ CLGALDGT+IKVNV +S      
Sbjct: 61  HFN----AVLRLHEILLKQPDPVTYSCSHEKWRWFQKCLGALDGTHIKVNVSMSDRPRYR 120

Query: 121 ----------------------IARGWEGSAADSRVLRDAISRPNGLTVPKGYYYLCDAG 180
                                 +  GWEGSA+DSRVLRDA+SR  GL VPKGYYYLCDAG
Sbjct: 121 SRKGDITTNVLGVCLQNGEFIFVMPGWEGSASDSRVLRDAVSRLTGLKVPKGYYYLCDAG 180

Query: 181 YPNAEGFLAPYRGERYHLSEWRG-------------------------------RWAILR 240
           YPNAEGFLAPYRG+RYHL+EWRG                               RW IL+
Sbjct: 181 YPNAEGFLAPYRGQRYHLTEWRGGNPPKCPKELFNMRHSFARNVIERAFGSLNCRWTILQ 240

Query: 241 GKSYYPVRIQ------------------------GGPSQHAAYFTILLIERWVTVKFLMS 277
           G+SYY V IQ                          P       + + IE    V+    
Sbjct: 241 GRSYYLVDIQCKIITACCLLHNLIHREMGSEATFKEPHLGEGDSSEMNIENINFVETTNV 300

BLAST of Lag0031442 vs. ExPASy TrEMBL
Match: A0A5A7SWD8 (Retrotransposon protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold515G00010 PE=3 SV=1)

HSP 1 Score: 359.4 bits (921), Expect = 1.5e-95
Identity = 196/354 (55.37%), Postives = 224/354 (63.28%), Query Frame = 0

Query: 1   MDRRCFAILCTLLKTVAGLSSTEVVDVEEMVAMFLHIVAHDVKNRVVRTQFARSGESVSR 60
           MDRRCFAILC LL+T+AGL+STEVVDVEEMVAMFLHI+AHDVKNRV++ +F RSGE++SR
Sbjct: 67  MDRRCFAILCHLLRTIAGLTSTEVVDVEEMVAMFLHILAHDVKNRVIQREFMRSGETISR 126

Query: 61  HFNTVLHVVLRLHDVLLKKPEPVTASCTDPRWKWFQNCLGALDGTYIKVNVVLS------ 120
           HFN VL  V+RLHD LLKKP+PV   CTD RW+WF+NCLGALDGTYIKVNV  S      
Sbjct: 127 HFNMVLLAVIRLHDELLKKPQPVPNECTDQRWRWFENCLGALDGTYIKVNVPASDRARYR 186

Query: 121 ----------------------IARGWEGSAADSRVLRDAISRPNGLTVPKGYYYLCDAG 180
                                 +  GWEGSAADSR+LRDA+SRPN L VPKGYYYL DAG
Sbjct: 187 TRKGEVATNVLGVYDTKGDFVYVLTGWEGSAADSRILRDALSRPNRLKVPKGYYYLVDAG 246

Query: 181 YPNAEGFLAPYRGERYHLSEWR--------------------------------GRWAIL 240
           YPNAEGFLAPYRG+RYHL EWR                                GRWAIL
Sbjct: 247 YPNAEGFLAPYRGQRYHLQEWRGPKNAPSTSKEFFNMKHSSARNVIERAFGVLKGRWAIL 306

Query: 241 RGKSYYPVRIQGGPSQHAAYFTIL---LIERWVTVKFLMSWMRWILLLLQLMNASSSRAP 292
           RGKSY+PV +Q     H      L   LI R +T   +   +        +   SSSR P
Sbjct: 307 RGKSYHPVEVQ----CHTILACCLLHNLINREMTNFDIEDNI--------VSMTSSSRLP 366

BLAST of Lag0031442 vs. ExPASy TrEMBL
Match: A0A5A7SYW1 (Retrotransposon protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold147G00430 PE=3 SV=1)

HSP 1 Score: 345.5 bits (885), Expect = 2.2e-91
Identity = 183/352 (51.99%), Postives = 221/352 (62.78%), Query Frame = 0

Query: 1   MDRRCFAILCTLLKTVAGLSSTEVVDVEEMVAMFLHIVAHDVKNRVVRTQFARSGESVSR 60
           MDRR FAILC LL+ VAGLSSTE+VDVEEMVAMFLHI AHDVKNRV++ +F RSGE+VSR
Sbjct: 40  MDRRTFAILCHLLRNVAGLSSTEIVDVEEMVAMFLHIFAHDVKNRVIQREFVRSGETVSR 99

Query: 61  HFNTVLHVVLRLHDVLLKKPEPVTASCTDPRWKWFQNCLGALDGTYIKVNV--------- 120
           HFN VL  VLRL++ L+K+P PVT++C D RWK F+NCLGALDGTYIKVNV         
Sbjct: 100 HFNIVLLAVLRLYEELIKRPVPVTSNCNDQRWKCFENCLGALDGTYIKVNVPAGDRPTFR 159

Query: 121 -------------------VLSIARGWEGSAADSRVLRDAISRPNGLTVPKGYYYLCDAG 180
                               + +  GW+GSAADSR+LRDAISR NGL VPKGYYYLCDAG
Sbjct: 160 TRKGEIATNVLGVCDTKGDFVYVLAGWKGSAADSRILRDAISRENGLQVPKGYYYLCDAG 219

Query: 181 YPNAEGFLAPYRGERYHLSEWR--------------------------------GRWAIL 240
           YPNAEGFLAPYRG+RYHL EWR                                GRWAIL
Sbjct: 220 YPNAEGFLAPYRGQRYHLQEWRGAANAPTNAKEYFNMKHSSARNVIERAFGVLKGRWAIL 279

Query: 241 RGKS--YYPVRIQGGPSQHAAYFTILLIERWVTVKFLMSWMRW-----ILLLLQLMNASS 286
           RGKS   Y   ++      + Y T    E    ++    W +W       + +    ++S
Sbjct: 280 RGKSEMTYCDDVEDEDEGDSTYATTTASEDIQYIETTNEWSQWRDDLAASMFIDWHMSTS 339

BLAST of Lag0031442 vs. ExPASy TrEMBL
Match: E5GCB5 (Retrotransposon protein OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1)

HSP 1 Score: 343.6 bits (880), Expect = 8.3e-91
Identity = 186/362 (51.38%), Postives = 217/362 (59.94%), Query Frame = 0

Query: 15  TVAGLSSTEVVDVEEMVAMFLHIVAHDVKNRVVRTQFARSGESVSRHFNTVLHVVLRLHD 74
           T+AGL+STEVVDVEEMVAMFLHI+AHDVK+RV++ +F RSGE++SRHFN VL  V+RLH+
Sbjct: 57  TIAGLTSTEVVDVEEMVAMFLHILAHDVKSRVIKREFMRSGETISRHFNMVLLAVIRLHE 116

Query: 75  VLLKKPEPVTASCTDPRWKWFQNCLGALDGTYIKVNVVLS-------------------- 134
            LLKKP+PV   CTD RW+WF+NCLGALDGTYIKVNV  S                    
Sbjct: 117 ELLKKPQPVPNECTDQRWRWFENCLGALDGTYIKVNVPASDRARYRTRKGEVATNVLGVC 176

Query: 135 --------IARGWEGSAADSRVLRDAISRPNGLTVPKGYYYLCDAGYPNAEGFLAPYRGE 194
                   +  GWEGSAADSR+LRDA+SRPN L VPKGYYYL D GYPNAEGFLAPYRG+
Sbjct: 177 DTKGDFVYVLAGWEGSAADSRILRDALSRPNRLKVPKGYYYLVDVGYPNAEGFLAPYRGQ 236

Query: 195 RYHLSEWR--------------------------------GRWAILRGKSYYPVRIQGGP 254
           RYHL EWR                                GRWAILRGKSYYPV +Q   
Sbjct: 237 RYHLQEWRGPENAPSTSKEFFNMKHYSARNVIERAFGVLKGRWAILRGKSYYPVEVQ-CR 296

Query: 255 SQHAAYFTILLIERWVT-------------------------VKFLMSWMRWILLLLQLM 292
           +  A      LI R +T                         ++    W +W   L + +
Sbjct: 297 TILACCLLHNLINREMTNFDIEDNIDEVDSTHATTAADDIHYIETSNEWSQWRDNLAEEI 356

BLAST of Lag0031442 vs. ExPASy TrEMBL
Match: A0A5D3BSN2 (Putative nuclease HARBI1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold145G00050 PE=3 SV=1)

HSP 1 Score: 325.9 bits (834), Expect = 1.8e-85
Identity = 173/313 (55.27%), Postives = 196/313 (62.62%), Query Frame = 0

Query: 1   MDRRCFAILCTLLKTVAGLSSTEVVDVEEMVAMFLHIVAHDVKNRVVRTQFARSGESVSR 60
           MDRRCF ILC LL+TVAGL+S EVVDVEEMVAMFLHIVAHDVKNRV++ +F RSGE++SR
Sbjct: 1   MDRRCFTILCHLLRTVAGLTSIEVVDVEEMVAMFLHIVAHDVKNRVIQREFMRSGETISR 60

Query: 61  HFNTVLHVVLRLHDVLLKKPEPVTASCTDPRWKWFQNCLGALDGTYIKVNVVLS------ 120
           HFN VL VV+RLHD LLKKP+PV   CTD RW+WF+NCLGALDGTYIKVNV  S      
Sbjct: 61  HFNMVLLVVIRLHDKLLKKPQPVNNDCTDQRWRWFENCLGALDGTYIKVNVPASDRARYR 120

Query: 121 ----------------------IARGWEGSAADSRVLRDAISRPNGLTVPKGYYYLCDAG 180
                                 +  GWEGSAADSR+L DAISRPNGL VPKGYYYL DAG
Sbjct: 121 THKGEVATNVLGLCDTKGDFVYVLAGWEGSAADSRILHDAISRPNGLKVPKGYYYLVDAG 180

Query: 181 YPNAEGFLAPYRGERYHLSEWRGRWAILRGKSYYPVRIQGGPSQHAAYFTILLIERWVTV 240
           YPN +GFLA YRG+RYHL EWRG              ++  PS    +F           
Sbjct: 181 YPNVDGFLAAYRGQRYHLQEWRG--------------VENAPSTSKEFFN---------- 240

Query: 241 KFLMSWMRWILLLLQLMNASSSRAPKHIWTKNEDAKLVESLVSLVHAGGWRSDNGTFKAG 286
                           M  SS+R      TK E+A LVE LV LV+ GGWRSDNGTF  G
Sbjct: 241 ----------------MKHSSARN-----TKEEEAGLVECLVELVNGGGWRSDNGTFHPG 268

BLAST of Lag0031442 vs. ExPASy TrEMBL
Match: A0A803QNC5 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 325.5 bits (833), Expect = 2.3e-85
Identity = 174/356 (48.88%), Postives = 210/356 (58.99%), Query Frame = 0

Query: 1   MDRRCFAILCTLLKTVAGLSSTEVVDVEEMVAMFLHIVAHDVKNRVVRTQFARSGESVSR 60
           MDRR F ILC  LKT  GL  ++ VDVEEMVA+FLHI+AHDVKNR+VR QFARSGE+VSR
Sbjct: 1   MDRRTFFILCHHLKTTGGLKGSKNVDVEEMVAIFLHIIAHDVKNRIVRRQFARSGETVSR 60

Query: 61  HFNTVLHVVLRLHDVLLKKPEPVTASCTDPRWKWFQNCLGALDGTYIKVNVVLS------ 120
           HFN VL+ +L LHD+LLKKP  +   C D RWKWF+NCLGALDGTYIKVNV+ S      
Sbjct: 61  HFNMVLNALLHLHDLLLKKPVAIRDDCIDERWKWFKNCLGALDGTYIKVNVLASNRPRYR 120

Query: 121 ----------------------IARGWEGSAADSRVLRDAISRPNGLTVPKGYYYLCDAG 180
                                 +  GWEGSAADSRVLRDAI R NG  VP+GYYYLCDAG
Sbjct: 121 TRKNEIATNVLGVVSQDMQFIYVLPGWEGSAADSRVLRDAIHR-NGFKVPQGYYYLCDAG 180

Query: 181 YPNAEGFLAPYRGERYHLSEW-----------------------------RGRWAILRGK 240
           YPN EGFL PYRG+RYHL++W                             +GRWAILR +
Sbjct: 181 YPNGEGFLTPYRGQRYHLNDWTHPPNSPREFFNMRHSSARNVVERAFGLLKGRWAILRSR 240

Query: 241 SYYPVRIQGGPSQHAAYFTILLIERWVTVKFLMSWMRWILLLLQLMNASSSRAP----KH 296
           SYYPV+IQ                               ++L  +M A+S   P    KH
Sbjct: 241 SYYPVKIQ-----------------------------CRIILGDIMEATSQSTPIGGRKH 300

BLAST of Lag0031442 vs. TAIR 10
Match: AT5G41980.1 (CONTAINS InterPro DOMAIN/s: Putative harbinger transposase-derived nuclease (InterPro:IPR006912); BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G43722.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink). )

HSP 1 Score: 119.8 bits (299), Expect = 3.8e-27
Identity = 70/194 (36.08%), Postives = 102/194 (52.58%), Query Frame = 0

Query: 1   MDRRCFAILCTLLKTVAGLSSTEVVDVEEMVAMFLHIVAHDVKNRVVRTQFARSGESVSR 60
           MD+  F  LC LL+T   L  T  + +E  +A+FL I+ H+++ R V+  F  SGE++SR
Sbjct: 48  MDKPVFYKLCDLLQTRGLLRHTNRIKIEAQLAIFLFIIGHNLRTRAVQELFCYSGETISR 107

Query: 61  HFNTVLHVVLRL-HDVLLKKPEPVTASCTDPRWKWFQNCLGALDGTYIKV---------- 120
           HFN VL+ V+ +  D         T    DP   +F++C+G +D  +I V          
Sbjct: 108 HFNNVLNAVIAISKDFFQPNSNSDTLENDDP---YFKDCVGVVDSFHIPVMVGVDEQGPF 167

Query: 121 ---------NVVLS---------IARGWEGSAADSRVLRDAISRPNGLTVPKGYYYLCDA 166
                    NV+ +         +  GWEGSA+D +VL  A++R N L VP+G YY+ D 
Sbjct: 168 RNGNGLLTQNVLAASSFDLRFNYVLAGWEGSASDQQVLNAALTRRNKLQVPQGKYYIVDN 227

BLAST of Lag0031442 vs. TAIR 10
Match: AT1G43722.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G28730.1); Has 924 Blast hits to 912 proteins in 109 species: Archae - 0; Bacteria - 0; Metazoa - 222; Fungi - 31; Plants - 661; Viruses - 0; Other Eukaryotes - 10 (source: NCBI BLink). )

HSP 1 Score: 92.8 bits (229), Expect = 4.9e-19
Identity = 67/214 (31.31%), Postives = 94/214 (43.93%), Query Frame = 0

Query: 1   MDRRCFAILCTLLKTVAGLSSTEVVDVEEMVAMFLHIVAHDVKNRVVRTQFARSGESVSR 60
           M   CF  LC +L+T   L  T  + +EE VAMFL I  H+   R V  +F R+ E+V R
Sbjct: 72  MSLPCFTTLCNMLQTNYDLQPTLNISIEESVAMFLRICGHNEVYRDVGLRFGRNQETVQR 131

Query: 61  HFNTVLHVVLRLHDVLLKKPE-------PVTASCTDPRWKWFQNCLGALDGTYIKVNV-- 120
            F  VL     L    ++ P        P         W +F   +GA+DGT++ V V  
Sbjct: 132 KFREVLTATELLACDYIRTPTRQELYRIPERLQVDQRYWPYFSGFVGAMDGTHVCVKVKP 191

Query: 121 ----------------VLSIA----------RGWEGSAADSRVLRDAISRPNGLTVPKG- 174
                           +++I            G  GS  D+ VL+ A    +   +P   
Sbjct: 192 DLQGMYWNRHDNASLNIMAICDLKMLFTYIWNGAPGSCYDTAVLQIAQQSDSEFPLPPSE 251

BLAST of Lag0031442 vs. TAIR 10
Match: AT5G28730.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G43722.1); Has 496 Blast hits to 496 proteins in 68 species: Archae - 0; Bacteria - 0; Metazoa - 3; Fungi - 23; Plants - 470; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 73.6 bits (179), Expect = 3.1e-13
Identity = 58/178 (32.58%), Postives = 83/178 (46.63%), Query Frame = 0

Query: 1   MDRRCFAILCTLLKTVAGLSSTEVVDVEEMVAMFLHIVAHDVKNRVVRTQFARSGESVSR 60
           M    F  LC +L    GL S+  + ++E VA+FL I A +   R +  +F  + E++ R
Sbjct: 30  MSSEAFTQLCEILHGKYGLQSSTNISLDESVAIFLIICASNDTQRDIALRFGHAQETIWR 89

Query: 61  HFNTVLHVVLRL--HDVLLKKPEPVTASCT----DPR-WKWFQNCLGALDGTYIKV---- 120
            F+ VL  + RL    +  +K E + A       D R W +  + LG      + +    
Sbjct: 90  KFHDVLKAMERLAVEYIRPRKVEELRAISNRLQDDTRYWPFLMDLLGIASFNVLAICDLD 149

Query: 121 NVVLSIARGWEGSAADSRVLRDAIS-RPNGLTVPKGYYYLCDAGYPNAEGFLAPYRGE 167
            +      G  GS  D+RVL  AIS  P     P   YYL D+GY N  G+LAPYR E
Sbjct: 150 MLFTYCFVGMAGSTHDARVLSAAISDDPLFHVPPDSKYYLVDSGYANKRGYLAPYRRE 207

BLAST of Lag0031442 vs. TAIR 10
Match: AT5G35695.1 (CONTAINS InterPro DOMAIN/s: Putative harbinger transposase-derived nuclease (InterPro:IPR006912); BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G41980.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 62.8 bits (151), Expect = 5.5e-10
Identity = 30/66 (45.45%), Postives = 40/66 (60.61%), Query Frame = 0

Query: 111 VVLSIARGWEGSAADSRVLRDAISRPNGLTVPKGYYYLCDAGYPNAEGFLAPYRGERYHL 170
           + + +  GWEGSA DSRVL DA+ +          +YL D G+ N   FLAP+RG RYHL
Sbjct: 24  IFIYVLSGWEGSAHDSRVLSDALRK----------FYLVDCGFANRLNFLAPFRGVRYHL 79

Query: 171 SEWRGR 177
            E+ G+
Sbjct: 84  QEFAGQ 79