Lag0031355 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0031355
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	2-oxoglutarate-dependent dioxygenase AOP3-like
Location	chr11: 7447273 .. 7450187 (+)
RNA-Seq Expression	Lag0031355
Synteny	Lag0031355

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGGTTTCAATCTTTGGCAGAGCCACAGGTTCCTGTTATCAACTTCTCAACTGAGAATCTTCATCCTCAAAGCACCAAATGGATGGAAACTTGCAGCCATGTGAGGGCAGCTCTTGAAGAATATGGCTGTTTTTTGGCTGAGTATGAGAGTTTCTCACAAGAGCTTGATGCTGCCATTTTCAAGGGTTTGGAAGTGCTTTTTGATTTGCCCTTAGAAACAAAAATGAAGAATGTCTCTGATAAGCCTTACCATGGATTTTTGGACCATAGGGCTCCATTTGTTCTTCCTCTACATGAAAGCCTTGGCATTGAAAATGCAGCAAGCTTTGAAGCTGTTGAATCTTTTGTCAACCTCTTGTGGCCTTCTGGAAATGGTCTTTTCTGGTATATATTTATATATATATGTATATGTATTTTGTTTGATTGATTGGTTTTTCTTTTTCTAATCTTTTGTTTTTGAATGTTCTTCATCTCATTGAATCATTGATCTCATATCTAAGTTATGCTTGATATAACAACTTTTGTATTTACTTTTCTTTACAATTTTGGTTGTTTTACTCATAATTTTTTTTTTTTTTAAAAAATTAAATTATGTTGGACAGTGAAAATGTATTTTCATACTCAAAGATGGTATCAGAGATGGAGCAATTAGTAAAGAGGATGGTGTTTGAAAGCTATGGAGTAGAAAAATACTATGATTCTCACATTGCTTCCACCACTTACCTCCTAAGAACCATGAAATATAGAATCCCTCAAATGGGTGAAGAGAATATTGGAGCTGCAGCTCACACAGACAAGAGTTTCTTCACCATTCTTCATCAAAATGGAGTGAAGGGCTTGGAAATCAAAGCAAAAATAGTGATCATTGGCTTGGAGTTCAATTCTCTCCAAATTCCTTCTTGGTCATGGCTGGAGATGCTTGTTTGGTATTACCTCTTATATGTTTTATATATATTGTTTACCTAGACATGAAATCCTATTATTTATGATCTCCATAAATCAATCATGTTTTGAATGTTAAAATTTGAGGATTTCTTTTTATAGAAACATGAAGCGTGTTTGATAACTATTTAGGTCTCGTTTGACAACCATTTGATTTTTGGTTTTTGAAAATTATGCTTGTTTTCTCCCAATTTCTTTAGAATGATTTTCATCTTTGTGAAAGAGACATTTGAATTTTTAGCCAAATTCTAAAAACAAAAACAAGTTTTTGAAAACTACTTTTTTTAGTTTTCATAAATTTGTTTGGGTTTTGAAAATATGGGAAGAAGGTGTGAGTAACATAACATAAAGAAACCTATAGGTAAAAGTTGTGTTTAATAAGCTTAATTTTAAAAAACCAAAAATAAAAAACCTAATGGTTATCAAACGGGACCTTAGTTTTTGGTTTTTTTGGTTTTTGAAAATTAAGCTTGTGAACACTATACTTCCACTCATAAATTTCTTTGTTTTGTTATCTAACTTCTTCCCATGTTTTCAAAAACCAAATCAAGTTTTGAAAACTAGAAAAAGTCGTAGTTTTCAAAAACTTGTTTCTGTTTTGGAATTTGGCTACGAAATCAAGTGTGTCCTAAACTAAGATGAAAACCATTGTGAGAAAACTGGAAGTAAACAATAATTTTCAAATAAAAAAGCAAATGGTTATCGAACAGGGTCCAAGATTCTTGTTTCTGAACTAGTTTTAGACATAAAACTAAGATGGGGAAAAATCATTGCCAAACAATTGTATAGTTTTTCTTTAACATTGTAACTTCTTTCTTTCTTAACAAGATTTAATTCTATAAATATTTTCTCAATTGTCTTATCCATATCATGCACAATTAATCAACAGTCTTTATCATGTTATTTAGATTTTAATGGTAAGGATTAATCAAATATGGCACTCATCCAAATTTGACAATGGGAGTATTATATTCTTTTATGGATAGGCATGGAGCAATGGGAGAATCCACTCAGCCACCCATAGAGTTATCATAGAGGGAACCAATGAAAGATATTCAACTGGCCTATTTTCATACCACAAAGGAATTATTGAGATTCCACATGAGTTGGTGGATGAACAACATCCCTTGAGATTTAACCCATTTAACCATTATGGATTACTTGGTTACTTTTCCACAGATGAGTGTGAGAAAATGGCTTCCACTGCCAAAGCTTATTGTGGAGTTTGAGAGAACTTTATATATGTTTATTGTAAAGAGTATGGTTGTTGGTGGAATAATAATGAAAATTTATCTTTAAACTTATATAACATATATGTTTATATTGTGCCAAATTTATTCTCAAGTTGAGTTGTAATGATTTATTTTTTTTTTTCATGTGTTGGATTTGTTGATTGGCCACCTTTGATCATTATATTGACATCTAAAAACTAGCTAATTTAATGAATTCTATTACCATTGTTTATAAAACATGAAGAGAAACACAAAGCAAACGAATTAATAATTCTCACGTTGATGCCAAAATCCAAGCAGGGAGAACTCACCTTAACCTTCACGTGACACCAAGGTAAATTTGTTTGAAGAGGGAGAAAACGTGGTCTGCAAGACAAGAAAATTTGTACACTGGTGTGGTGCTTGCCACACCGCCTCCGATACTTAAGTCAACAAGGAGATAGATGAGAAGCAAGTAAAAGAGCAAAGGAAGAGGAGGGAATTCAGAGAATGAGTTTCGGGATCCGGTTTATATACCTTTTTTCGCCCTCTTTTTAGGGTTCCTAAAGCGTCTGAAGACAGAAGCGACCTAAACCAGATCGAACTGGGTTCTCCAAGGACACTAGAAGCAAAACAGAGGGGAAAGGGACTCAACCCATCGAAGTGGCCCGAGACCGAGGAGCCCAATCCCTTTGATCCTCGGCTCTTTCGAGGTCTTTCCTTGGATTCGTGTCGTGCTATCCTCGTCTCTACTCCTTAG

mRNA sequence

ATGGGGTTTCAATCTTTGGCAGAGCCACAGGTTCCTGTTATCAACTTCTCAACTGAGAATCTTCATCCTCAAAGCACCAAATGGATGGAAACTTGCAGCCATGTGAGGGCAGCTCTTGAAGAATATGGCTGTTTTTTGGCTGAGTATGAGAGTTTCTCACAAGAGCTTGATGCTGCCATTTTCAAGGGTTTGGAAGTGCTTTTTGATTTGCCCTTAGAAACAAAAATGAAGAATGTCTCTGATAAGCCTTACCATGGATTTTTGGACCATAGGGCTCCATTTGTTCTTCCTCTACATGAAAGCCTTGGCATTGAAAATGCAGCAAGCTTTGAAGCTGTTGAATCTTTTGTCAACCTCTTGTGGCCTTCTGGAAATGGTCTTTTCTGTGAAAATGTATTTTCATACTCAAAGATGGTATCAGAGATGGAGCAATTAGTAAAGAGGATGGTGTTTGAAAGCTATGGAGTAGAAAAATACTATGATTCTCACATTGCTTCCACCACTTACCTCCTAAGAACCATGAAATATAGAATCCCTCAAATGGGTGAAGAGAATATTGGAGCTGCAGCTCACACAGACAAGAGTTTCTTCACCATTCTTCATCAAAATGGAGTGAAGGGCTTGGAAATCAAAGCAAAAATAGTGATCATTGGCTTGGAGTTCAATTCTCTCCAAATTCCTTCTTGGTCATGGCTGGAGATGCTTGTTTGGGTTCCTAAAGCGTCTGAAGACAGAAGCGACCTAAACCAGATCGAACTGGGTTCTCCAAGGACACTAGAAGCAAAACAGAGGGGAAAGGGACTCAACCCATCGAAGTGGCCCGAGACCGAGGAGCCCAATCCCTTTGATCCTCGGCTCTTTCGAGGTCTTTCCTTGGATTCGTGTCGTGCTATCCTCGTCTCTACTCCTTAG

Coding sequence (CDS)

ATGGGGTTTCAATCTTTGGCAGAGCCACAGGTTCCTGTTATCAACTTCTCAACTGAGAATCTTCATCCTCAAAGCACCAAATGGATGGAAACTTGCAGCCATGTGAGGGCAGCTCTTGAAGAATATGGCTGTTTTTTGGCTGAGTATGAGAGTTTCTCACAAGAGCTTGATGCTGCCATTTTCAAGGGTTTGGAAGTGCTTTTTGATTTGCCCTTAGAAACAAAAATGAAGAATGTCTCTGATAAGCCTTACCATGGATTTTTGGACCATAGGGCTCCATTTGTTCTTCCTCTACATGAAAGCCTTGGCATTGAAAATGCAGCAAGCTTTGAAGCTGTTGAATCTTTTGTCAACCTCTTGTGGCCTTCTGGAAATGGTCTTTTCTGTGAAAATGTATTTTCATACTCAAAGATGGTATCAGAGATGGAGCAATTAGTAAAGAGGATGGTGTTTGAAAGCTATGGAGTAGAAAAATACTATGATTCTCACATTGCTTCCACCACTTACCTCCTAAGAACCATGAAATATAGAATCCCTCAAATGGGTGAAGAGAATATTGGAGCTGCAGCTCACACAGACAAGAGTTTCTTCACCATTCTTCATCAAAATGGAGTGAAGGGCTTGGAAATCAAAGCAAAAATAGTGATCATTGGCTTGGAGTTCAATTCTCTCCAAATTCCTTCTTGGTCATGGCTGGAGATGCTTGTTTGGGTTCCTAAAGCGTCTGAAGACAGAAGCGACCTAAACCAGATCGAACTGGGTTCTCCAAGGACACTAGAAGCAAAACAGAGGGGAAAGGGACTCAACCCATCGAAGTGGCCCGAGACCGAGGAGCCCAATCCCTTTGATCCTCGGCTCTTTCGAGGTCTTTCCTTGGATTCGTGTCGTGCTATCCTCGTCTCTACTCCTTAG

Protein sequence

MGFQSLAEPQVPVINFSTENLHPQSTKWMETCSHVRAALEEYGCFLAEYESFSQELDAAIFKGLEVLFDLPLETKMKNVSDKPYHGFLDHRAPFVLPLHESLGIENAASFEAVESFVNLLWPSGNGLFCENVFSYSKMVSEMEQLVKRMVFESYGVEKYYDSHIASTTYLLRTMKYRIPQMGEENIGAAAHTDKSFFTILHQNGVKGLEIKAKIVIIGLEFNSLQIPSWSWLEMLVWVPKASEDRSDLNQIELGSPRTLEAKQRGKGLNPSKWPETEEPNPFDPRLFRGLSLDSCRAILVSTP

Homology

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BLAST of Lag0031355 vs. NCBI nr
Match: XP_023525673.1 (2-oxoglutarate-dependent dioxygenase AOP3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 374.4 bits (960), Expect = 9.3e-100
Identity = 185/227 (81.50%), Postives = 201/227 (88.55%), Query Frame = 0


Query: 1   MGFQSLAEPQVPVINFSTENLHPQSTKWMETCSHVRAALEEYGCFLAEYESFSQ---ELD 60
           MGF+S   P+VPVI+FS ENL P+STKW+ETCSHVRAALEEYGCFLAEYE+  +   ELD
Sbjct: 1   MGFRSSTNPEVPVISFSIENLRPKSTKWVETCSHVRAALEEYGCFLAEYENDVEQEVELD 60

Query: 61  AAIFKGLEVLFDLPLETKMKNVSDKPYHGFLDHRAPFVLPLHESLGIENAASFEAVESFV 120
            AIF GLE LFDLPLETKMKNVSDKPYHGFLDHRAPFVLPLHES GIENAASFE VESFV
Sbjct: 61  DAIFNGLEKLFDLPLETKMKNVSDKPYHGFLDHRAPFVLPLHESFGIENAASFEGVESFV 120

Query: 121 NLLWPSGNGLFCENVFSYSKMVSEMEQLVKRMVFESYGVEKYYDSHIASTTYLLRTMKYR 180
            L+WPSGN  FCE++F+Y+KMVSEME+LVKRMVFESYGVEKYYDSHIASTTYLLR MKYR
Sbjct: 121 KLMWPSGNHHFCEDLFAYTKMVSEMEKLVKRMVFESYGVEKYYDSHIASTTYLLRGMKYR 180

Query: 181 IPQMGEENIGAAAHTDKSFFTILHQNGVKGLEIKAK--IVIIGLEFN 223
           IP+MGE+NIGA AHTDKSFFTILHQNGV GLEIKAK     IGL+FN
Sbjct: 181 IPEMGEQNIGAEAHTDKSFFTILHQNGVNGLEIKAKKDDHWIGLQFN 227

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BLAST of Lag0031355 vs. NCBI nr
Match: XP_022948698.1 (probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucurbita moschata])

HSP 1 Score: 374.0 bits (959), Expect = 1.2e-99
Identity = 184/228 (80.70%), Postives = 203/228 (89.04%), Query Frame = 0


Query: 1   MGFQSLAEPQVPVINFSTENLHPQSTKWMETCSHVRAALEEYGCFLAEYESFSQ---ELD 60
           MGF+S  +P+VPVINFS ENL P+STKW+ETC+HVRAALEEYGCFLAEYE+  +   ELD
Sbjct: 1   MGFRSSTKPEVPVINFSIENLGPKSTKWVETCTHVRAALEEYGCFLAEYENDVEQEVELD 60

Query: 61  AAIFKGLEVLFDLPLETKMKNVSDKPYHGFLDHRAPFVLPLHESLGIENAASFEAVESFV 120
            AIF GLE LFDLPL+TKMKNVSDKPYHGFLDHRAPFVLPLHES GIENAASFE VESFV
Sbjct: 61  DAIFNGLEKLFDLPLQTKMKNVSDKPYHGFLDHRAPFVLPLHESFGIENAASFEGVESFV 120

Query: 121 NLLWPSGNGLFCENVFSYSKMVSEMEQLVKRMVFESYGVEKYYDSHIASTTYLLRTMKYR 180
            L+WPSGN  FC+++F+Y+KMVSEME+LVKRMVFESYGVEKYYDSHIASTTYLLR MKYR
Sbjct: 121 KLMWPSGNHHFCQDLFAYTKMVSEMEKLVKRMVFESYGVEKYYDSHIASTTYLLRGMKYR 180

Query: 181 IPQMGEENIGAAAHTDKSFFTILHQNGVKGLEIKAK--IVIIGLEFNS 224
           IP+MGE+NIGA AHTDKSFFTILHQNGV GLEIKAK     IGL+FNS
Sbjct: 181 IPEMGEQNIGAEAHTDKSFFTILHQNGVNGLEIKAKKDDHWIGLQFNS 228

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BLAST of Lag0031355 vs. NCBI nr
Match: XP_022997882.1 (2-oxoglutarate-dependent dioxygenase AOP3-like [Cucurbita maxima])

HSP 1 Score: 372.9 bits (956), Expect = 2.7e-99
Identity = 184/224 (82.14%), Postives = 200/224 (89.29%), Query Frame = 0


Query: 1   MGFQSLAEPQVPVINFSTENLHPQSTKWMETCSHVRAALEEYGCFLAEYESFSQELDAAI 60
           MGF+S  +P+VP INFS ENL P+STKW+ETCSHVRAALEEYGCFLAE E+ + ELD AI
Sbjct: 1   MGFRSSKKPEVPAINFSIENLRPKSTKWVETCSHVRAALEEYGCFLAECEN-NVELDDAI 60

Query: 61  FKGLEVLFDLPLETKMKNVSDKPYHGFLDHRAPFVLPLHESLGIENAASFEAVESFVNLL 120
           F GLE LFDLPLETKMKNVSDKPYHGFLDHRAPFVLPLHES GIENAASFE VESFV L+
Sbjct: 61  FNGLEKLFDLPLETKMKNVSDKPYHGFLDHRAPFVLPLHESFGIENAASFEGVESFVKLM 120

Query: 121 WPSGNGLFCENVFSYSKMVSEMEQLVKRMVFESYGVEKYYDSHIASTTYLLRTMKYRIPQ 180
           WPSGN  FCE++F+Y+KMVSEME+LVKRMVFESYGVEKYYDSHIASTTYLLR MKYRIP+
Sbjct: 121 WPSGNHHFCEDLFAYTKMVSEMEKLVKRMVFESYGVEKYYDSHIASTTYLLRGMKYRIPE 180

Query: 181 MGEENIGAAAHTDKSFFTILHQNGVKGLEIKAK--IVIIGLEFN 223
           MGE+NIGA AHTDKSFFTILHQNGV GLEIKAK     IGL+FN
Sbjct: 181 MGEQNIGAEAHTDKSFFTILHQNGVNGLEIKAKKDDHWIGLQFN 223

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BLAST of Lag0031355 vs. NCBI nr
Match: KAG6606775.1 (putative 2-oxoglutarate-dependent dioxygenase AOP1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 368.6 bits (945), Expect = 5.1e-98
Identity = 177/216 (81.94%), Postives = 195/216 (90.28%), Query Frame = 0


Query: 1   MGFQSLAEPQVPVINFSTENLHPQSTKWMETCSHVRAALEEYGCFLAEYESFSQ---ELD 60
           MGF+S  +P+VPVINFS ENL P+STKW+ETC+HVRAALEEYGCFLAEYE+  +   ELD
Sbjct: 1   MGFRSSTKPEVPVINFSIENLRPKSTKWVETCTHVRAALEEYGCFLAEYENDVEQEVELD 60

Query: 61  AAIFKGLEVLFDLPLETKMKNVSDKPYHGFLDHRAPFVLPLHESLGIENAASFEAVESFV 120
            AIF GLE LFDLPL+TKMKNVSDKPYHGFLDHRAPFVLPLHES GIENAASFE VESFV
Sbjct: 61  DAIFNGLEKLFDLPLQTKMKNVSDKPYHGFLDHRAPFVLPLHESFGIENAASFEGVESFV 120

Query: 121 NLLWPSGNGLFCENVFSYSKMVSEMEQLVKRMVFESYGVEKYYDSHIASTTYLLRTMKYR 180
            L+WPSGN  FC+++F+Y+KMVSEME+LVKRMVFESYGV KYYDSHIASTTYLLR MKYR
Sbjct: 121 KLMWPSGNHHFCQDLFAYTKMVSEMEKLVKRMVFESYGVGKYYDSHIASTTYLLRGMKYR 180

Query: 181 IPQMGEENIGAAAHTDKSFFTILHQNGVKGLEIKAK 214
           IP+MGE+NIGA AHTDKSFFTILHQNGV GLEIKAK
Sbjct: 181 IPEMGEQNIGAEAHTDKSFFTILHQNGVNGLEIKAK 216

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BLAST of Lag0031355 vs. NCBI nr
Match: XP_038878200.1 (2-oxoglutarate-dependent dioxygenase AOP3-like [Benincasa hispida])

HSP 1 Score: 363.6 bits (932), Expect = 1.6e-96
Identity = 180/217 (82.95%), Postives = 192/217 (88.48%), Query Frame = 0


Query: 1   MGFQSLAEPQVPVINFSTEN---LHPQSTKWMETCSHVRAALEEYGCFLAEYESFSQELD 60
           MG QS A+ +VPVI FS EN   LHPQSTKW+ETCS VRAALEEYGCFLAEYES S +L 
Sbjct: 1   MGIQSSAKSEVPVIKFSIENDEDLHPQSTKWLETCSQVRAALEEYGCFLAEYESASSKLY 60

Query: 61  AAIFKGLEVLFDLPLETKMKNVSDKPYHGFLDHRAPFVLPLHESLGIENAASFEAVESFV 120
             IFKGL+ LFDLPLETKMKNVSDKPYHGFLDH APFVLPLH+SLGIENA SF+AVESF+
Sbjct: 61  DEIFKGLKELFDLPLETKMKNVSDKPYHGFLDHTAPFVLPLHQSLGIENATSFQAVESFL 120

Query: 121 NLLWPSGNGLFCENVFSYSKMVSEMEQLVKRMVFESYGVEKYYDSHIASTTYLLRTMKYR 180
            LLWPSGN  FCEN+FSYS+M+SEMEQLVK+MVFESYGV KYYDSHIASTTYLLRTMKYR
Sbjct: 121 KLLWPSGNHHFCENLFSYSEMISEMEQLVKKMVFESYGVGKYYDSHIASTTYLLRTMKYR 180

Query: 181 IPQMGEENIGAAAHTDKSFFTILHQ-NGVKGLEIKAK 214
           IPQMGEENIGA AHTDKSFFTILHQ NGV GLEIK K
Sbjct: 181 IPQMGEENIGAEAHTDKSFFTILHQINGVNGLEIKPK 217

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BLAST of Lag0031355 vs. ExPASy Swiss-Prot
Match: Q9ZTA3 (Probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Arabidopsis thaliana OX=3702 GN=AOP1 PE=2 SV=1)

HSP 1 Score: 191.4 bits (485), Expect = 1.5e-47
Identity = 95/211 (45.02%), Postives = 134/211 (63.51%), Query Frame = 0


Query: 10  QVPVINFSTENLHPQSTKWMETCSHVRAALEEYGCFLAEYESFSQELDAAIFKGLEVLFD 69
           Q+PVI+FS +NL P S+KW E  + V  ALE+YGCF A ++  S EL+ ++F+ +E LF+
Sbjct: 13  QLPVIDFSDQNLKPGSSKWDEVTADVLKALEDYGCFEASFDKLSVELNRSVFEAMEDLFE 72

Query: 70  LPLETKMKNVSDKPYHGFLDHRAPFVLPLHESLGIENAASFEAVESFVNLLWPS-GNGLF 129
           LP+ TK +NVS KP+HG+L H       L+ESLGI +A   E V  F   LWP  GN   
Sbjct: 73  LPIPTKQRNVSSKPFHGYLCHN------LYESLGINDANVLEKVNDFTQQLWPDHGNKSI 132

Query: 130 CENVFSYSKMVSEMEQLVKRMVFESYGVEKYYDSHIASTTYLLRTMKYRIPQMGEEN--- 189
            E +  +S+ + E++ +V+RM+ ES+G+E Y D H+ ST YL R MKY  P   +++   
Sbjct: 133 SETIHLFSEQLVELDLMVRRMIMESFGIENYIDEHLNSTYYLTRLMKYTSPPDDDDDDDE 192

Query: 190 ---IGAAAHTDKSFFTILHQNGVKGLEIKAK 214
              +G  +HTDK+  TILHQ  V GLE+K K
Sbjct: 193 ETKLGLRSHTDKNIITILHQYQVDGLEVKTK 217

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BLAST of Lag0031355 vs. ExPASy Swiss-Prot
Match: Q945B6 (Probable 2-oxoglutarate-dependent dioxygenase AOP1.2 OS=Arabidopsis thaliana OX=3702 GN=AOP1.2 PE=2 SV=1)

HSP 1 Score: 190.7 bits (483), Expect = 2.5e-47
Identity = 95/210 (45.24%), Postives = 135/210 (64.29%), Query Frame = 0


Query: 10  QVPVINFSTENLHPQSTKWMETCSHVRAALEEYGCFLAEYESFSQELDAAIFKGLEVLFD 69
           Q+PVI+FS +NL P S+KW E  + V  ALE+YGCF A ++  S EL+ ++F+ +E LF+
Sbjct: 13  QLPVIDFSDQNLKPGSSKWDEVKADVLKALEDYGCFEAFFDKLSVELNRSVFEAMEDLFE 72

Query: 70  LPLETKMKNVSDKPYHGFLDHRAPFVLPLHESLGIENAASFEAVESFVNLLWPS-GNGLF 129
           LP+ TK +NVS KP+HG+L H       L+ESLGI++A   E V  F   LWP  GN   
Sbjct: 73  LPIPTKQRNVSSKPFHGYLCHN------LYESLGIDDANVLEKVNDFTQQLWPDHGNKSI 132

Query: 130 CENVFSYSKMVSEMEQLVKRMVFESYGVEKYYDSHIASTTYLLRTMKYRIPQMGEEN--- 189
            E +  +S+ + E++ +V+RM+ ES+G+E Y D H+ ST YL R MKY  P   +++   
Sbjct: 133 SETIHLFSEQLVELDLMVRRMIMESFGIENYIDEHLNSTYYLTRLMKYTSPPDDDDDDEE 192

Query: 190 --IGAAAHTDKSFFTILHQNGVKGLEIKAK 214
             +G  +HTDK+  TILHQ  V GLE+K K
Sbjct: 193 TKLGLRSHTDKNIITILHQYQVDGLEVKTK 216

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BLAST of Lag0031355 vs. ExPASy Swiss-Prot
Match: Q944Z5 (Probable 2-oxoglutarate-dependent dioxygenase AOP1 (Fragment) OS=Arabidopsis thaliana OX=3702 GN=AOP1 PE=2 SV=1)

HSP 1 Score: 190.3 bits (482), Expect = 3.3e-47
Identity = 95/209 (45.45%), Postives = 134/209 (64.11%), Query Frame = 0


Query: 10  QVPVINFSTENLHPQSTKWMETCSHVRAALEEYGCFLAEYESFSQELDAAIFKGLEVLFD 69
           Q+PVI+FS +NL P S+KW E  + V  ALE+YGCF A ++  S EL+ ++F+ +E LF+
Sbjct: 6   QLPVIDFSDQNLKPGSSKWDEVTADVLKALEDYGCFEASFDKLSVELNRSVFEAMEDLFE 65

Query: 70  LPLETKMKNVSDKPYHGFLDHRAPFVLPLHESLGIENAASFEAVESFVNLLWPS-GNGLF 129
           LP+ TK +NVS K +HG+L H       L+ESLGI +A   E V  F   LWP  GN   
Sbjct: 66  LPIPTKQRNVSSKLFHGYLCHN------LYESLGINDANVLEKVNDFTQQLWPDHGNKSI 125

Query: 130 CENVFSYSKMVSEMEQLVKRMVFESYGVEKYYDSHIASTTYLLRTMKYRIPQMGEEN--- 189
            E +  +S+ + E++ +V+RM+ ES+G+EKY D H+ ST YL R MKY  P   +++   
Sbjct: 126 SETIHLFSEQLVELDLMVRRMIMESFGIEKYIDEHLNSTYYLTRLMKYTSPPDDDDDEET 185

Query: 190 -IGAAAHTDKSFFTILHQNGVKGLEIKAK 214
            +G  +HTDK+  TILHQ  V GLE+K K
Sbjct: 186 KLGLRSHTDKNIITILHQYQVDGLEVKTK 208

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BLAST of Lag0031355 vs. ExPASy Swiss-Prot
Match: A0A0N9HQ36 (Deoxypodophyllotoxin synthase OS=Sinopodophyllum hexandrum OX=93608 GN=2-ODD PE=1 SV=1)

HSP 1 Score: 180.3 bits (456), Expect = 3.4e-44
Identity = 92/209 (44.02%), Postives = 126/209 (60.29%), Query Frame = 0


Query: 5   SLAEPQVPVINFSTENLHPQSTKWMETCSHVRAALEEYGCFLAEYESFSQELDAAIFKGL 64
           S A  ++PVI+ S +NL P +T W    + VR ALEEYGCF A  ++ S EL  A+    
Sbjct: 3   STAPLRLPVIDLSMKNLKPGTTSWNSVRTQVREALEEYGCFEAVIDAVSPELQKAVCNKG 62

Query: 65  EVLFDLPLETKMKNVSDKPYHGFLDHRAPFVLPLHESLGIENAASFEAVESFVNLLWPSG 124
             L +LPLETKM N +   Y GF       +  L+E +G+      E VE F NL+WP G
Sbjct: 63  HELLNLPLETKMLNGNKPEYDGFTS-----IPNLNEGMGVGRITDLEKVERFTNLMWPEG 122

Query: 125 NGLFCENVFSYSKMVSEMEQLVKRMVFESYGVEKYYDSHIASTTYLLRTMKYRIPQMGEE 184
           N  FCE V+SY K ++E++ ++K MVFES+G+EK++DS   ST YLL  M+Y+ P     
Sbjct: 123 NKDFCETVYSYGKRMAEVDHILKMMVFESFGMEKHFDSFCESTNYLLHFMRYQQPGKDGR 182

Query: 185 NIGAAAHTDKSFFTILHQNGVKGLEIKAK 214
           +   + H DKS  TI++QN VKGLE + K
Sbjct: 183 SPALSLHKDKSILTIVNQNDVKGLEFETK 206

[Close]

BLAST of Lag0031355 vs. ExPASy Swiss-Prot
Match: Q945B5 (2-oxoglutarate-dependent dioxygenase AOP2 OS=Arabidopsis thaliana OX=3702 GN=AOP2 PE=2 SV=1)

HSP 1 Score: 162.2 bits (409), Expect = 9.5e-39
Identity = 88/210 (41.90%), Postives = 125/210 (59.52%), Query Frame = 0


Query: 10  QVPVINFSTENLHPQSTKWMETCSHVRAALEEYGCFLAEYESFSQELDAAIFKGLEVLFD 69
           Q+P+IN + + L P S+KW E  S VR ALE++GCF A Y+  S EL  +I K +E LF 
Sbjct: 7   QLPLINLADKTLEPGSSKWAEVRSDVRKALEDFGCFEASYDKVSLELQESIMKTMEELFA 66

Query: 70  LPLETKMKNVSDKPYHGFLDHRAPFVLPLHESLGIENAASFEAVESFVNLLWPSGNG--L 129
           LP+ETK +NV  KPY G+L+H       L ESLGI NA   E V  F   LWP G+G   
Sbjct: 67  LPVETKQRNVCPKPYVGYLNHN-----NLSESLGISNANILENVNEFTQQLWPDGDGNEN 126

Query: 130 FCENVFSYSKMVSEMEQLVKRMVFESYGVEKYYDSHIASTTYLLRTMKYRIPQMGEENIG 189
             + +  +++ + E++ +V+RMV ES+G+EKY D H+ ST Y +R MKY  P  G+ N  
Sbjct: 127 ISKTIQLFAEKLVEIDVMVRRMVMESFGIEKYIDDHLKSTEYRMRLMKYIAPPEGDANTT 186

Query: 190 AAAHTDKSFFTILHQNGVK---GLEIKAKI 215
              + D      L+ +GV+   G+++ A I
Sbjct: 187 VDDYAD--LLAKLNIDGVEPNVGVKVNADI 209

[Close]

BLAST of Lag0031355 vs. ExPASy TrEMBL
Match: A0A6J1GA17 (probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucurbita moschata OX=3662 GN=LOC111452291 PE=3 SV=1)

HSP 1 Score: 374.0 bits (959), Expect = 5.9e-100
Identity = 184/228 (80.70%), Postives = 203/228 (89.04%), Query Frame = 0


Query: 1   MGFQSLAEPQVPVINFSTENLHPQSTKWMETCSHVRAALEEYGCFLAEYESFSQ---ELD 60
           MGF+S  +P+VPVINFS ENL P+STKW+ETC+HVRAALEEYGCFLAEYE+  +   ELD
Sbjct: 1   MGFRSSTKPEVPVINFSIENLGPKSTKWVETCTHVRAALEEYGCFLAEYENDVEQEVELD 60

Query: 61  AAIFKGLEVLFDLPLETKMKNVSDKPYHGFLDHRAPFVLPLHESLGIENAASFEAVESFV 120
            AIF GLE LFDLPL+TKMKNVSDKPYHGFLDHRAPFVLPLHES GIENAASFE VESFV
Sbjct: 61  DAIFNGLEKLFDLPLQTKMKNVSDKPYHGFLDHRAPFVLPLHESFGIENAASFEGVESFV 120

Query: 121 NLLWPSGNGLFCENVFSYSKMVSEMEQLVKRMVFESYGVEKYYDSHIASTTYLLRTMKYR 180
            L+WPSGN  FC+++F+Y+KMVSEME+LVKRMVFESYGVEKYYDSHIASTTYLLR MKYR
Sbjct: 121 KLMWPSGNHHFCQDLFAYTKMVSEMEKLVKRMVFESYGVEKYYDSHIASTTYLLRGMKYR 180

Query: 181 IPQMGEENIGAAAHTDKSFFTILHQNGVKGLEIKAK--IVIIGLEFNS 224
           IP+MGE+NIGA AHTDKSFFTILHQNGV GLEIKAK     IGL+FNS
Sbjct: 181 IPEMGEQNIGAEAHTDKSFFTILHQNGVNGLEIKAKKDDHWIGLQFNS 228

[Close]

BLAST of Lag0031355 vs. ExPASy TrEMBL
Match: A0A6J1K6A3 (2-oxoglutarate-dependent dioxygenase AOP3-like OS=Cucurbita maxima OX=3661 GN=LOC111492707 PE=3 SV=1)

HSP 1 Score: 372.9 bits (956), Expect = 1.3e-99
Identity = 184/224 (82.14%), Postives = 200/224 (89.29%), Query Frame = 0


Query: 1   MGFQSLAEPQVPVINFSTENLHPQSTKWMETCSHVRAALEEYGCFLAEYESFSQELDAAI 60
           MGF+S  +P+VP INFS ENL P+STKW+ETCSHVRAALEEYGCFLAE E+ + ELD AI
Sbjct: 1   MGFRSSKKPEVPAINFSIENLRPKSTKWVETCSHVRAALEEYGCFLAECEN-NVELDDAI 60

Query: 61  FKGLEVLFDLPLETKMKNVSDKPYHGFLDHRAPFVLPLHESLGIENAASFEAVESFVNLL 120
           F GLE LFDLPLETKMKNVSDKPYHGFLDHRAPFVLPLHES GIENAASFE VESFV L+
Sbjct: 61  FNGLEKLFDLPLETKMKNVSDKPYHGFLDHRAPFVLPLHESFGIENAASFEGVESFVKLM 120

Query: 121 WPSGNGLFCENVFSYSKMVSEMEQLVKRMVFESYGVEKYYDSHIASTTYLLRTMKYRIPQ 180
           WPSGN  FCE++F+Y+KMVSEME+LVKRMVFESYGVEKYYDSHIASTTYLLR MKYRIP+
Sbjct: 121 WPSGNHHFCEDLFAYTKMVSEMEKLVKRMVFESYGVEKYYDSHIASTTYLLRGMKYRIPE 180

Query: 181 MGEENIGAAAHTDKSFFTILHQNGVKGLEIKAK--IVIIGLEFN 223
           MGE+NIGA AHTDKSFFTILHQNGV GLEIKAK     IGL+FN
Sbjct: 181 MGEQNIGAEAHTDKSFFTILHQNGVNGLEIKAKKDDHWIGLQFN 223

[Close]

BLAST of Lag0031355 vs. ExPASy TrEMBL
Match: A0A6J1DH70 (2-oxoglutarate-dependent dioxygenase AOP3-like OS=Momordica charantia OX=3673 GN=LOC111021051 PE=3 SV=1)

HSP 1 Score: 355.1 bits (910), Expect = 2.8e-94
Identity = 169/209 (80.86%), Postives = 186/209 (89.00%), Query Frame = 0


Query: 5   SLAEPQVPVINFSTENLHPQSTKWMETCSHVRAALEEYGCFLAEYESFSQELDAAIFKGL 64
           SLA+ ++PVIN S EN+ P+ST W+E CSH+RAALEEYGCFL E+ S + ELD  IFKGL
Sbjct: 6   SLAKAKLPVINLSIENVSPESTNWLEICSHLRAALEEYGCFLGEFGSATPELDDEIFKGL 65

Query: 65  EVLFDLPLETKMKNVSDKPYHGFLDHRAPFVLPLHESLGIENAASFEAVESFVNLLWPSG 124
           E LFDLPLETKMKNVSDKPYHGFLDHR PFVLPLH+S GIEN+ SFEAV+SFVNL+WPSG
Sbjct: 66  EELFDLPLETKMKNVSDKPYHGFLDHRTPFVLPLHQSFGIENSESFEAVQSFVNLMWPSG 125

Query: 125 NGLFCENVFSYSKMVSEMEQLVKRMVFESYGVEKYYDSHIASTTYLLRTMKYRIPQMGEE 184
           N  FCENVFSY+KMVSEME+LVK MVFESYGV+KYYDSHIASTTYLLRTMKYRIP+MGEE
Sbjct: 126 NRRFCENVFSYTKMVSEMEKLVKVMVFESYGVKKYYDSHIASTTYLLRTMKYRIPEMGEE 185

Query: 185 NIGAAAHTDKSFFTILHQNGVKGLEIKAK 214
           NIGA AHTDKSFFTILHQN V GLEIK K
Sbjct: 186 NIGADAHTDKSFFTILHQNEVNGLEIKTK 214

[Close]

BLAST of Lag0031355 vs. ExPASy TrEMBL
Match: A0A0A0LBU1 (Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G555690 PE=4 SV=1)

HSP 1 Score: 304.7 bits (779), Expect = 4.4e-79
Identity = 159/234 (67.95%), Postives = 182/234 (77.78%), Query Frame = 0


Query: 1   MGFQSLAEPQVPVINFST-----ENLHPQSTK-WMETCSHVRAALEEYGCFLAEYE---- 60
           MG   LA+ +VP+I  S      ++  P+S K W+E    VR ALEEYGCFL EY+    
Sbjct: 1   MGIDCLAKLEVPIIKLSNVDKKDDDSDPESRKLWLERSKQVRDALEEYGCFLGEYDDDES 60

Query: 61  ------SFSQELDA----AIFKGLEVLFDLPLETKMKNVSDKPYHGFLDHRAPFVLPLHE 120
                 S S +L++     IFKGL+ LFDLPLETK+KNVSDKPYHGFLD R PFVLPLH+
Sbjct: 61  SCSCNSSSSPKLNSNNYEEIFKGLKELFDLPLETKIKNVSDKPYHGFLDCRTPFVLPLHQ 120

Query: 121 SLGIENAASFEAVESFVNLLWPSGNGLFCENVFSYSKMVSEMEQLVKRMVFESYGVEKYY 180
           SLGIENA+S+EAV+ FVNLLWPSGN  FCE +F+YSK +SEMEQLVKRMVFESYGV KYY
Sbjct: 121 SLGIENASSYEAVQFFVNLLWPSGNHHFCEKLFAYSKKISEMEQLVKRMVFESYGVGKYY 180

Query: 181 DSHIASTTYLLRTMKYRIPQMGEENIGAAAHTDKSFFTILHQ-NGVKGLEIKAK 214
           DSHIASTTYLLRTMKYRIP+MGEENIGA AHTDKSFFTILHQ +GV GL+IK K
Sbjct: 181 DSHIASTTYLLRTMKYRIPKMGEENIGAEAHTDKSFFTILHQFDGVNGLQIKPK 234

[Close]

BLAST of Lag0031355 vs. ExPASy TrEMBL
Match: W9STP2 (Gibberellin 3-beta-dioxygenase 4 OS=Morus notabilis OX=981085 GN=L484_019467 PE=3 SV=1)

HSP 1 Score: 266.9 bits (681), Expect = 1.0e-67
Identity = 122/206 (59.22%), Postives = 159/206 (77.18%), Query Frame = 0


Query: 9   PQVPVINFSTENLHPQSTKWMETCSHVRAALEEYGCFLAEYESFSQELDAAIFKGLEVLF 68
           P++P I+FS  NL+P ++ W   C+ VR ALEEYGCF+A Y+  SQ+L  ++F  L+ LF
Sbjct: 7   PKIPTIDFSEGNLNPGTSSWFSVCNDVRRALEEYGCFVALYDKISQQLRTSMFSELKELF 66

Query: 69  DLPLETKMKNVSDKPYHGFLDHRAPFVLPLHESLGIENAASFEAVESFVNLLWP-SGNGL 128
           DLP E KMKNVSDKPY G++ +  P + PLHES+GIENA + E  ESF N++WP  GN  
Sbjct: 67  DLPTEIKMKNVSDKPYFGYIGNH-PSIPPLHESMGIENATTLEGGESFTNIIWPKGGNNH 126

Query: 129 FCENVFSYSKMVSEMEQLVKRMVFESYGVEKYYDSHIASTTYLLRTMKYRIPQMGEENIG 188
           FCE V SYS++VSE+E++VKRMVF+SYGVEKYYDSH+ STT+LL+ +KYR+P+M E +IG
Sbjct: 127 FCETVVSYSRLVSELEKMVKRMVFQSYGVEKYYDSHLGSTTFLLKLIKYRVPEMNESSIG 186

Query: 189 AAAHTDKSFFTILHQNGVKGLEIKAK 214
            A HTDKSF T+LHQN V GLEI+ +
Sbjct: 187 CAPHTDKSFITVLHQNEVNGLEIETR 211

[Close]

BLAST of Lag0031355 vs. TAIR 10
Match: AT1G52800.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 214.9 bits (546), Expect = 8.8e-56
Identity = 105/230 (45.65%), Postives = 148/230 (64.35%), Query Frame = 0


Query: 6   LAEPQVPVINF-STENLHPQSTKWMETCSHVRAALEEYGCFLAEYESFSQELDAAIFKGL 65
           L   +VP+++  S + L P ++ W         ALEEYGCFLA Y+  +Q+LD +IF   
Sbjct: 5   LKTTKVPILDLTSQQELKPNTSTWRSVSREACEALEEYGCFLAVYDGVTQQLDDSIFAAA 64

Query: 66  EVLFDLPLETKMKNVSDKPYHGFLDHRAPFVLPLHESLGIENAASFEAVESFVNLLWPSG 125
           E LFDLP ETK KNV++KPYHG++      V+PLHE LG++   + E  + F +L+WP G
Sbjct: 65  EELFDLPTETKKKNVNEKPYHGYVGQMP--VIPLHEGLGVDYVTNKEIAQRFTHLMWPQG 124

Query: 126 NGLFCENVFSYSKMVSEMEQLVKRMVFESYGVEKYYDSHIASTTYLLRTMKYRIPQMGEE 185
           N  FC  V ++S  V+E+++LV RM+FE+YGVEK+Y+SH+ S TYLL+ +KY  P     
Sbjct: 125 NDRFCNTVHTFSNAVAELDRLVVRMIFENYGVEKHYESHVGSKTYLLKFLKYLAPPESIS 184

Query: 186 NIGAAAHTDKSFFTILHQNGVKGLEIKAKIVIIGLEFNSLQIPSWSWLEM 235
                 HTDK+F +ILHQN V GLE+K+K      E+ SLQ+P  S++ M
Sbjct: 185 MPAFPQHTDKTFLSILHQNDVNGLEVKSK----DGEWISLQLPPKSYVVM 228

[Close]

BLAST of Lag0031355 vs. TAIR 10
Match: AT1G52790.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 207.2 bits (526), Expect = 1.8e-53
Identity = 102/206 (49.51%), Postives = 139/206 (67.48%), Query Frame = 0


Query: 9   PQVPVINFSTENLHPQSTKWMETCSHVRAALEEYGCFLAEYESFSQ-ELDAAIFKGLEVL 68
           P++P ++FS E+L P +  W  T  ++R ALEEYGCF+ + +  +  +L   +F  L  L
Sbjct: 3   PKIPTLDFSREDLKPGTKYWESTRENIRQALEEYGCFIIDLKDKTPLDLLDRVFGSLVDL 62

Query: 69  FDLPLETKMKNVSDKPYHGFLDHRAPFVLPLHESLGIENAASFEAVESFVNLLWPSGNGL 128
           FDLP +TKMKN  DKP +G++  + P  LPLHESLGI+NA S EA  SF  L+WP GN  
Sbjct: 63  FDLPTQTKMKNKYDKPLNGYVG-QIP-ALPLHESLGIDNATSLEATRSFTGLMWPQGNEH 122

Query: 129 FCENVFSYSKMVSEMEQLVKRMVFESYGVEKYYDSHIASTTYLLRTMKYRIPQMGEENIG 188
           F E ++ Y++  +E++Q+V RMVF+SY VEKYYD +I STTYLLR +K R P      +G
Sbjct: 123 FSECLYKYAEFAAELDQMVTRMVFQSYNVEKYYDPYIESTTYLLRVLKNRAPNNENPTLG 182

Query: 189 AAAHTDKSFFTILHQNGVKGLEIKAK 214
              HTDKSF TILHQ+ V GLE++ +
Sbjct: 183 FVTHTDKSFTTILHQDQVNGLEMETR 206

[Close]

BLAST of Lag0031355 vs. TAIR 10
Match: AT1G52820.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 198.0 bits (502), Expect = 1.1e-50
Identity = 93/204 (45.59%), Postives = 140/204 (68.63%), Query Frame = 0


Query: 10  QVPVINFSTENLHPQSTKWMETCSHVRAALEEYGCFLAEYESFSQELDAAIFKGLEVLFD 69
           ++PVI+FS +NL P   +W  T + V+ AL++YG F A ++    EL  ++F  LE LFD
Sbjct: 11  RLPVIDFSNKNLKPGEPEWDLTRADVQKALQDYGYFEASFDRIPFELRKSVFGALEELFD 70

Query: 70  LPLETKMKNVSDKPYHGFLDHRAPFVLPLHESLGIENAASFEAVESFVNLLWPSGNGLFC 129
           LPL+TK++NVS KP+HG++      ++PL+ES+GI+++   E V++F   LWP GN  F 
Sbjct: 71  LPLQTKLRNVSKKPFHGYVGQYP--MVPLYESMGIDDSDIAEKVDAFTEKLWPQGNISFS 130

Query: 130 ENVFSYSKMVSEMEQLVKRMVFESYGVEKYYDSHIASTTYLLRTMKYRIPQMGEENIGAA 189
             + S+SK +SE++  ++RM+ ES+G++KY D H+ ST YLLR MKY+ P   E  +G  
Sbjct: 131 TTIQSFSKKLSELDITIRRMIMESFGLDKYIDEHLHSTNYLLRVMKYKGPDTEETKVGLN 190

Query: 190 AHTDKSFFTILHQNGVKGLEIKAK 214
           AHTDK+  TIL+QN V+GLE++ K
Sbjct: 191 AHTDKNIVTILYQNHVEGLEVQTK 212

[Close]

BLAST of Lag0031355 vs. TAIR 10
Match: AT4G03070.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 191.4 bits (485), Expect = 1.0e-48
Identity = 95/211 (45.02%), Postives = 134/211 (63.51%), Query Frame = 0


Query: 10  QVPVINFSTENLHPQSTKWMETCSHVRAALEEYGCFLAEYESFSQELDAAIFKGLEVLFD 69
           Q+PVI+FS +NL P S+KW E  + V  ALE+YGCF A ++  S EL+ ++F+ +E LF+
Sbjct: 13  QLPVIDFSDQNLKPGSSKWDEVTADVLKALEDYGCFEASFDKLSVELNRSVFEAMEDLFE 72

Query: 70  LPLETKMKNVSDKPYHGFLDHRAPFVLPLHESLGIENAASFEAVESFVNLLWPS-GNGLF 129
           LP+ TK +NVS KP+HG+L H       L+ESLGI +A   E V  F   LWP  GN   
Sbjct: 73  LPIPTKQRNVSSKPFHGYLCHN------LYESLGINDANVLEKVNDFTQQLWPDHGNKSI 132

Query: 130 CENVFSYSKMVSEMEQLVKRMVFESYGVEKYYDSHIASTTYLLRTMKYRIPQMGEEN--- 189
            E +  +S+ + E++ +V+RM+ ES+G+E Y D H+ ST YL R MKY  P   +++   
Sbjct: 133 SETIHLFSEQLVELDLMVRRMIMESFGIENYIDEHLNSTYYLTRLMKYTSPPDDDDDDDE 192

Query: 190 ---IGAAAHTDKSFFTILHQNGVKGLEIKAK 214
              +G  +HTDK+  TILHQ  V GLE+K K
Sbjct: 193 ETKLGLRSHTDKNIITILHQYQVDGLEVKTK 217

[Close]

BLAST of Lag0031355 vs. TAIR 10
Match: AT1G15540.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 176.0 bits (445), Expect = 4.5e-44
Identity = 90/207 (43.48%), Postives = 126/207 (60.87%), Query Frame = 0


Query: 10  QVPVINFSTENLHPQSTKWMETCSHVRAALEEYGCFLAEYESFSQELDAAIFKGLEVLFD 69
           ++P+IN S +NL   +  W  T   VR A+E +G F+AEY +F   L  +I +  + L D
Sbjct: 8   ELPIINLSDKNLKQNTELWSFTRDSVREAMEHHGWFVAEYNNFPTGLHQSILEAAKELLD 67

Query: 70  LPLETKMKNVSDKPYHGFLDHRAPFVLPLHESLGIENAASFEAVESFVNLLWP---SGNG 129
           LP+E K+KN + K  HG++   +    P+HE LGI+     +    F  L+WP     N 
Sbjct: 68  LPVEIKLKNENHKAGHGYITMMSD-GQPVHEGLGIDQVNDVQQCRGFSRLMWPDDHDDND 127

Query: 130 LFCENVFSYSKMVSEMEQLVKRMVFESYGVEKYYDSHIASTTYLLRTMKYRIPQMGEENI 189
            FCE V +Y+KM +E+EQLV RM+FESY VEKY + +I  T YLLR +KYR    GE N 
Sbjct: 128 RFCETVHAYAKMQAELEQLVIRMLFESYNVEKYTEKYIGGTRYLLRLLKYRRLPNGEPNR 187

Query: 190 GAAAHTDKSFFTILHQNGVKGLEIKAK 214
              +HTDKSF +ILHQN + GL +K++
Sbjct: 188 KFISHTDKSFISILHQNHITGLMLKSE 213

The following BLAST results are available for this feature:

BLAST of Lag0031355 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023525673.1	9.3e-100	81.50	2-oxoglutarate-dependent dioxygenase AOP3-like [Cucurbita pepo subsp. pepo]	[more]
XP_022948698.1	1.2e-99	80.70	probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucurbita moschata]	[more]
XP_022997882.1	2.7e-99	82.14	2-oxoglutarate-dependent dioxygenase AOP3-like [Cucurbita maxima]	[more]
KAG6606775.1	5.1e-98	81.94	putative 2-oxoglutarate-dependent dioxygenase AOP1, partial [Cucurbita argyrospe...	[more]
XP_038878200.1	1.6e-96	82.95	2-oxoglutarate-dependent dioxygenase AOP3-like [Benincasa hispida]	[more]

BLAST of Lag0031355 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9ZTA3	1.5e-47	45.02	Probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Arabidopsis thaliana OX=37...	[more]
Q945B6	2.5e-47	45.24	Probable 2-oxoglutarate-dependent dioxygenase AOP1.2 OS=Arabidopsis thaliana OX=...	[more]
Q944Z5	3.3e-47	45.45	Probable 2-oxoglutarate-dependent dioxygenase AOP1 (Fragment) OS=Arabidopsis tha...	[more]
A0A0N9HQ36	3.4e-44	44.02	Deoxypodophyllotoxin synthase OS=Sinopodophyllum hexandrum OX=93608 GN=2-ODD PE=...	[more]
Q945B5	9.5e-39	41.90	2-oxoglutarate-dependent dioxygenase AOP2 OS=Arabidopsis thaliana OX=3702 GN=AOP...	[more]

BLAST of Lag0031355 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GA17	5.9e-100	80.70	probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucurbita moschata OX=3662...	[more]
A0A6J1K6A3	1.3e-99	82.14	2-oxoglutarate-dependent dioxygenase AOP3-like OS=Cucurbita maxima OX=3661 GN=LO...	[more]
A0A6J1DH70	2.8e-94	80.86	2-oxoglutarate-dependent dioxygenase AOP3-like OS=Momordica charantia OX=3673 GN...	[more]
A0A0A0LBU1	4.4e-79	67.95	Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G...	[more]
W9STP2	1.0e-67	59.22	Gibberellin 3-beta-dioxygenase 4 OS=Morus notabilis OX=981085 GN=L484_019467 PE=...	[more]

BLAST of Lag0031355 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G52800.1	8.8e-56	45.65	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT1G52790.1	1.8e-53	49.51	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT1G52820.1	1.1e-50	45.59	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT4G03070.1	1.0e-48	45.02	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT1G15540.1	4.5e-44	43.48	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR027443	Isopenicillin N synthase-like superfamily	GENE3D	2.60.120.330		coord: 3..231 e-value: 3.1E-30 score: 107.9
IPR026992	Non-haem dioxygenase N-terminal domain	PFAM	PF14226	DIOX_N	coord: 11..87 e-value: 3.4E-9 score: 37.5
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 253..281
None	No IPR available	PANTHER	PTHR47990	2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATED	coord: 5..214
None	No IPR available	PANTHER	PTHR47990:SF17	2-OXOGLUTARATE-DEPENDENT DIOXYGENASE AOP3-LIKE	coord: 5..214
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 8..211

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0031355.1	Lag0031355.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0051213	dioxygenase activity
molecular_function	GO:0046872	metal ion binding