Lag0031335 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0031335
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	lys-63-specific deubiquitinase BRCC36-like
Location	chr11: 7164316 .. 7165487 (-)
RNA-Seq Expression	Lag0031335
Synteny	Lag0031335

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTCATGGATCGGTATCAACGGTACGCTGAACCGTACTGTATTGCTGCTCGAGAAGCCTTGCGATCCCCTGCTCTGCACAAGGTCCTTCGAAACACCGGCCAGTGAGTCTCGCTTCAGAGTTGAAAGCTCGAAGATCCTTTCGAATCTCTCTCTTTCTTTCTCCCTTTTCCTTCATTCCTTTTCTGCACAGAGAATCGAAGTTTCCGATTTCGGAGAAAAAAAACGGCGATCCAAAGATGTCTCTGACGGGCGTGAAGATGTCCGAAGACGTTTGGTTTACTTGTCTCACTCATGCATTGTCCACCGAGACTGAGGAAATCATGGGGCTTCTTCTTGGCGATATCGAGGTCACTCTCTAGATTCCCACTCTGCAATTTGCTACCCCTCCTCTTTTAAGGGTTTTTTTTTTTCGAGTTTGTTTCAGCTCATTCTCGCCCATTTTGCGTAATCTGCTGTGGGTTTTGCTTTAATTTTGGTTATTGGGGGTGTATTCGTTGGCTTGGTTGCTTGTATTGGACGTCTGTTTGGAAGAAGGGTCAATTTGGTTGTTTTCGGTTGTTTTGGGAAATGGGGTTGTTTTTGGTTTGGATTCTCTTTATTCTAGAGGTATGAGTTTTGTGCTCCCTTGGCTTGGTTAGTTGGTTCCTTGGTCATTTTGTAAAATCTACAGGGAGGAGTGGTGTTTATGGTACTCTGTAGAATCTTTGACTCCAAATGATGTCTGTTAGAATTTAGAAATGGACTTGGTTTGTTTTTCCACAAGAGTTCGTCTGAGTTTGTTACATTAAGCACTGTTCTGATCCTCTGTTATAGCTCTTGTTAGATGGTCTATCAGAATTGTGGTCAATTGGTTCATGGCATAGAATAGAGGATGTTAATTTTAAAATTTATCTCTACAGTTTTGAGTAGTTGTTTTCATGGCTGATTCTCTAAGCTTTAAGCTCTCTCATGCCCTTTCTATTGGGTGCAGTTATGTGTTCTGTTCTCACAATGAAATTTTGCAGCATTCAAAAAATGGTAGTGTAACTGCACTAATTTGGGGAGCATCGCCTCAGACACGGTCTGACAGAAGGAAGGACCGTGTGGAGACTCATCCTGAACAACTGGCTGCTGCATCTGCCCAGGCTGAGATATCCTTTGCTTCTTTTATCATTATGAAATTCTTGTAG

mRNA sequence

ATGGTCATGGATCGGTATCAACGGTACGCTGAACCGTACTGTATTGCTGCTCGAGAAGCCTTGCGATCCCCTGCTCTGCACAAGGTCCTTCGAAACACCGGCCAAGAATCGAAGTTTCCGATTTCGGAGAAAAAAAACGGCGATCCAAAGATGTCTCTGACGGGCGTGAAGATGTCCGAAGACGTTTGGTTTACTTGTCTCACTCATGCATTGTCCACCGAGACTGAGGAAATCATGGGGCTTCTTCTTGGCGATATCGAGCATTCAAAAAATGGTAGTGTAACTGCACTAATTTGGGGAGCATCGCCTCAGACACGGTCTGACAGAAGGAAGGACCGTGTGGAGACTCATCCTGAACAACTGGCTGCTGCATCTGCCCAGGCTGAGATATCCTTTGCTTCTTTTATCATTATGAAATTCTTGTAG

Coding sequence (CDS)

ATGGTCATGGATCGGTATCAACGGTACGCTGAACCGTACTGTATTGCTGCTCGAGAAGCCTTGCGATCCCCTGCTCTGCACAAGGTCCTTCGAAACACCGGCCAAGAATCGAAGTTTCCGATTTCGGAGAAAAAAAACGGCGATCCAAAGATGTCTCTGACGGGCGTGAAGATGTCCGAAGACGTTTGGTTTACTTGTCTCACTCATGCATTGTCCACCGAGACTGAGGAAATCATGGGGCTTCTTCTTGGCGATATCGAGCATTCAAAAAATGGTAGTGTAACTGCACTAATTTGGGGAGCATCGCCTCAGACACGGTCTGACAGAAGGAAGGACCGTGTGGAGACTCATCCTGAACAACTGGCTGCTGCATCTGCCCAGGCTGAGATATCCTTTGCTTCTTTTATCATTATGAAATTCTTGTAG

Protein sequence

MVMDRYQRYAEPYCIAAREALRSPALHKVLRNTGQESKFPISEKKNGDPKMSLTGVKMSEDVWFTCLTHALSTETEEIMGLLLGDIEHSKNGSVTALIWGASPQTRSDRRKDRVETHPEQLAAASAQAEISFASFIIMKFL

Homology

[Close]

BLAST of Lag0031335 vs. NCBI nr
Match: KAG6606786.1 (Lys-63-specific deubiquitinase BRCC36, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 173.3 bits (438), Expect = 1.5e-39
Identity = 87/90 (96.67%), Postives = 88/90 (97.78%), Query Frame = 0


Query: 51  MSLTGVKMSEDVWFTCLTHALSTETEEIMGLLLGDIEHSKNGSVTALIWGASPQTRSDRR 110
           MSLT VKMSEDVWFTCLTHALSTETEEIMGLLLGDIEHSKNGSVTALIWGASPQTRSDRR
Sbjct: 1   MSLTAVKMSEDVWFTCLTHALSTETEEIMGLLLGDIEHSKNGSVTALIWGASPQTRSDRR 60

Query: 111 KDRVETHPEQLAAASAQAEISFASFIIMKF 141
           KDRVETHPEQLAAASAQA+ISFA FIIMKF
Sbjct: 61  KDRVETHPEQLAAASAQADISFAPFIIMKF 90

[Close]

BLAST of Lag0031335 vs. NCBI nr
Match: XP_022153609.1 (lys-63-specific deubiquitinase BRCC36 isoform X2 [Momordica charantia])

HSP 1 Score: 166.4 bits (420), Expect = 1.8e-37
Identity = 81/87 (93.10%), Postives = 86/87 (98.85%), Query Frame = 0


Query: 43  EKKNGDPKMSLTGVKMSEDVWFTCLTHALSTETEEIMGLLLGDIEHSKNGSVTALIWGAS 102
           +++NGD KMSLTGVKMSEDVWFTCLTHALSTETEEIMGLLLGDIEHS+NG+VTALIWGAS
Sbjct: 56  KEQNGDQKMSLTGVKMSEDVWFTCLTHALSTETEEIMGLLLGDIEHSRNGNVTALIWGAS 115

Query: 103 PQTRSDRRKDRVETHPEQLAAASAQAE 130
           PQTRSDRRKDRVETHPEQLAAASAQAE
Sbjct: 116 PQTRSDRRKDRVETHPEQLAAASAQAE 142

[Close]

BLAST of Lag0031335 vs. NCBI nr
Match: XP_022153607.1 (lys-63-specific deubiquitinase BRCC36 isoform X1 [Momordica charantia])

HSP 1 Score: 166.4 bits (420), Expect = 1.8e-37
Identity = 81/87 (93.10%), Postives = 86/87 (98.85%), Query Frame = 0


Query: 43  EKKNGDPKMSLTGVKMSEDVWFTCLTHALSTETEEIMGLLLGDIEHSKNGSVTALIWGAS 102
           +++NGD KMSLTGVKMSEDVWFTCLTHALSTETEEIMGLLLGDIEHS+NG+VTALIWGAS
Sbjct: 56  KEQNGDQKMSLTGVKMSEDVWFTCLTHALSTETEEIMGLLLGDIEHSRNGNVTALIWGAS 115

Query: 103 PQTRSDRRKDRVETHPEQLAAASAQAE 130
           PQTRSDRRKDRVETHPEQLAAASAQAE
Sbjct: 116 PQTRSDRRKDRVETHPEQLAAASAQAE 142

[Close]

BLAST of Lag0031335 vs. NCBI nr
Match: XP_023528524.1 (lys-63-specific deubiquitinase BRCC36-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 164.5 bits (415), Expect = 6.8e-37
Identity = 81/85 (95.29%), Postives = 83/85 (97.65%), Query Frame = 0


Query: 45  KNGDPKMSLTGVKMSEDVWFTCLTHALSTETEEIMGLLLGDIEHSKNGSVTALIWGASPQ 104
           +NGD KMSLT VKMSEDVWFTCLTHALSTETEEIMGLLLGDIEHS+NGSVTALIWGASPQ
Sbjct: 47  RNGDRKMSLTSVKMSEDVWFTCLTHALSTETEEIMGLLLGDIEHSENGSVTALIWGASPQ 106

Query: 105 TRSDRRKDRVETHPEQLAAASAQAE 130
           TRSDRRKDRVETHPEQLAAASAQAE
Sbjct: 107 TRSDRRKDRVETHPEQLAAASAQAE 131

[Close]

BLAST of Lag0031335 vs. NCBI nr
Match: XP_023528526.1 (COP9 signalosome complex subunit 5-like isoform X3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 164.5 bits (415), Expect = 6.8e-37
Identity = 81/85 (95.29%), Postives = 83/85 (97.65%), Query Frame = 0


Query: 45  KNGDPKMSLTGVKMSEDVWFTCLTHALSTETEEIMGLLLGDIEHSKNGSVTALIWGASPQ 104
           +NGD KMSLT VKMSEDVWFTCLTHALSTETEEIMGLLLGDIEHS+NGSVTALIWGASPQ
Sbjct: 47  RNGDRKMSLTSVKMSEDVWFTCLTHALSTETEEIMGLLLGDIEHSENGSVTALIWGASPQ 106

Query: 105 TRSDRRKDRVETHPEQLAAASAQAE 130
           TRSDRRKDRVETHPEQLAAASAQAE
Sbjct: 107 TRSDRRKDRVETHPEQLAAASAQAE 131

[Close]

BLAST of Lag0031335 vs. ExPASy Swiss-Prot
Match: B5X8M4 (Lys-63-specific deubiquitinase BRCC36 OS=Salmo salar OX=8030 GN=brcc3 PE=2 SV=1)

HSP 1 Score: 67.8 bits (164), Expect = 1.1e-10
Identity = 33/79 (41.77%), Postives = 54/79 (68.35%), Query Frame = 0


Query: 51  MSLTGVKMSEDVWFTCLTHALSTETEEIMGLLLGDIEHSKNGSVTALIWGASPQTRSDRR 110
           M+++ V +  D +  C+ HALSTE EE+MGL +G+++ ++   + ++I       RSD+R
Sbjct: 1   MAVSAVHLESDAFLVCMNHALSTEKEEVMGLCIGEVDTNRIVHIHSVI----ILRRSDKR 60

Query: 111 KDRVETHPEQLAAASAQAE 130
           KDRVE  PEQL++A+ +AE
Sbjct: 61  KDRVEISPEQLSSAATEAE 75

[Close]

BLAST of Lag0031335 vs. ExPASy Swiss-Prot
Match: Q4VA72 (Lys-63-specific deubiquitinase BRCC36 OS=Xenopus tropicalis OX=8364 GN=brcc3 PE=2 SV=1)

HSP 1 Score: 67.0 bits (162), Expect = 1.9e-10
Identity = 34/79 (43.04%), Postives = 53/79 (67.09%), Query Frame = 0


Query: 51  MSLTGVKMSEDVWFTCLTHALSTETEEIMGLLLGDIEHSKNGSVTALIWGASPQTRSDRR 110
           M++  V +  D +  C+TH+LSTE EE+MGL +G+++  K   + ++I       RSD+R
Sbjct: 1   MAVQAVHIQGDAFLVCVTHSLSTEREEVMGLCIGEVDTQKVVHIHSVI----ILRRSDKR 60

Query: 111 KDRVETHPEQLAAASAQAE 130
           KDRVE  PEQL+AA+ +A+
Sbjct: 61  KDRVEISPEQLSAATTEAD 75

[Close]

BLAST of Lag0031335 vs. ExPASy Swiss-Prot
Match: Q66GV6 (Lys-63-specific deubiquitinase BRCC36 OS=Xenopus laevis OX=8355 GN=brcc3 PE=2 SV=1)

HSP 1 Score: 66.6 bits (161), Expect = 2.5e-10
Identity = 34/79 (43.04%), Postives = 53/79 (67.09%), Query Frame = 0


Query: 51  MSLTGVKMSEDVWFTCLTHALSTETEEIMGLLLGDIEHSKNGSVTALIWGASPQTRSDRR 110
           M++  V +  D +  C+TH+LSTE EE+MGL +G+++  K   + ++I       RSD+R
Sbjct: 1   MAVQAVHIQGDAFLVCVTHSLSTEREEVMGLCIGEVDTQKLVHIHSVI----ILRRSDKR 60

Query: 111 KDRVETHPEQLAAASAQAE 130
           KDRVE  PEQL+AA+ +A+
Sbjct: 61  KDRVEISPEQLSAATIEAD 75

[Close]

BLAST of Lag0031335 vs. ExPASy Swiss-Prot
Match: B0KWU8 (Lys-63-specific deubiquitinase BRCC36 OS=Callithrix jacchus OX=9483 GN=BRCC3 PE=3 SV=2)

HSP 1 Score: 62.4 bits (150), Expect = 4.8e-09
Identity = 36/98 (36.73%), Postives = 48/98 (48.98%), Query Frame = 0


Query: 52  SLTGVKMSEDVWFTCLTHALSTETEEIMGLLLGDIEHSKNGSVTALIWGASPQT------ 111
           ++  V +  D +  CL HALSTE EE+MGL +G++             G   +T      
Sbjct: 8   AVQAVHLESDAFLVCLNHALSTEKEEVMGLCIGELNDDTRSDSKFAYTGTEMRTVAEKVD 67

Query: 112 --------------RSDRRKDRVETHPEQLAAASAQAE 130
                         RSD+RKDRVE  PEQL+AAS +AE
Sbjct: 68  AVRIVHIHSVIILRRSDKRKDRVEISPEQLSAASTEAE 105

[Close]

BLAST of Lag0031335 vs. ExPASy Swiss-Prot
Match: P46736 (Lys-63-specific deubiquitinase BRCC36 OS=Homo sapiens OX=9606 GN=BRCC3 PE=1 SV=2)

HSP 1 Score: 62.4 bits (150), Expect = 4.8e-09
Identity = 36/98 (36.73%), Postives = 48/98 (48.98%), Query Frame = 0


Query: 52  SLTGVKMSEDVWFTCLTHALSTETEEIMGLLLGDIEHSKNGSVTALIWGASPQT------ 111
           ++  V +  D +  CL HALSTE EE+MGL +G++             G   +T      
Sbjct: 8   AVQAVHLESDAFLVCLNHALSTEKEEVMGLCIGELNDDTRSDSKFAYTGTEMRTVAEKVD 67

Query: 112 --------------RSDRRKDRVETHPEQLAAASAQAE 130
                         RSD+RKDRVE  PEQL+AAS +AE
Sbjct: 68  AVRIVHIHSVIILRRSDKRKDRVEISPEQLSAASTEAE 105

The following BLAST results are available for this feature:

BLAST of Lag0031335 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6606786.1	1.5e-39	96.67	Lys-63-specific deubiquitinase BRCC36, partial [Cucurbita argyrosperma subsp. so...	[more]
XP_022153609.1	1.8e-37	93.10	lys-63-specific deubiquitinase BRCC36 isoform X2 [Momordica charantia]	[more]
XP_022153607.1	1.8e-37	93.10	lys-63-specific deubiquitinase BRCC36 isoform X1 [Momordica charantia]	[more]
XP_023528524.1	6.8e-37	95.29	lys-63-specific deubiquitinase BRCC36-like isoform X1 [Cucurbita pepo subsp. pep...	[more]
XP_023528526.1	6.8e-37	95.29	COP9 signalosome complex subunit 5-like isoform X3 [Cucurbita pepo subsp. pepo]	[more]

BLAST of Lag0031335 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
B5X8M4	1.1e-10	41.77	Lys-63-specific deubiquitinase BRCC36 OS=Salmo salar OX=8030 GN=brcc3 PE=2 SV=1	[more]
Q4VA72	1.9e-10	43.04	Lys-63-specific deubiquitinase BRCC36 OS=Xenopus tropicalis OX=8364 GN=brcc3 PE=...	[more]
Q66GV6	2.5e-10	43.04	Lys-63-specific deubiquitinase BRCC36 OS=Xenopus laevis OX=8355 GN=brcc3 PE=2 SV...	[more]
B0KWU8	4.8e-09	36.73	Lys-63-specific deubiquitinase BRCC36 OS=Callithrix jacchus OX=9483 GN=BRCC3 PE=...	[more]
P46736	4.8e-09	36.73	Lys-63-specific deubiquitinase BRCC36 OS=Homo sapiens OX=9606 GN=BRCC3 PE=1 SV=2	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000555	JAB1/MPN/MOV34 metalloenzyme domain	PFAM	PF01398	JAB	coord: 54..128 e-value: 2.1E-7 score: 31.0
None	No IPR available	GENE3D	3.40.140.10	Cytidine Deaminase, domain 2	coord: 50..135 e-value: 3.7E-18 score: 67.5
None	No IPR available	PANTHER	PTHR10410	EUKARYOTIC TRANSLATION INITIATION FACTOR 3 -RELATED	coord: 51..131
None	No IPR available	PANTHER	PTHR10410:SF2	LYS-63-SPECIFIC DEUBIQUITINASE BRCC36-RELATED	coord: 51..131

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0031335.1	Lag0031335.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006302	double-strand break repair
biological_process	GO:0070537	histone H2A K63-linked deubiquitination
cellular_component	GO:0070531	BRCA1-A complex
cellular_component	GO:0070552	BRISC complex
molecular_function	GO:0070122	isopeptidase activity
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0008237	metallopeptidase activity
molecular_function	GO:0031593	polyubiquitin modification-dependent protein binding
molecular_function	GO:0004843	thiol-dependent deubiquitinase
molecular_function	GO:0005515	protein binding