Lag0031294 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0031294
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	Exonuclease 1
Location	chr11: 6739408 .. 6741173 (-)
RNA-Seq Expression	Lag0031294
Synteny	Lag0031294

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCCTCATGTAAATAATCTCACTTTTCACCACAGGACAAGAAAAGAAAATTTCGCACGTGCAAGAGAACATGAAACAAATGGCAATTCTGTTGCTACCTACGAGTGCTACCAGAAGGTCGTTGATATAACACCTTTGATCGCCCATTAACTAATTCAAGTAACAACTTTTTCATATTTGTTATGTTACTATTGTTAGAGGGTTGTGTGAATTATATTTTTGAGTGCTCAAGGATATTACTTTAAACTTGACATGAGTTCATTTCAGTGCTCAAGGATATTACTTTAAACTTGACATGAGTTCATTTTGAAGATGTTAAAGCGAGAGAATATATCATATGTTGTGGCACCTTATGAGGCAGATGCTAAAAATGACCTTCTTGGTTATCAGCAATCAGGTTGATGTCGTTCTTACTGAGGACTCTGATCTAATACCGTTTGGTTGTCCAAGGGTAAGCTATATAGAAAAACATTCTTGGACTCTTTTCATTTGTCAATCCACTTTGATATTCTTAGATGTAAACTGCTTCTACTCTATACTTCATGGAGAATTCTTAATGATTTTTGTTGCTTTGCTCCTGTACAAATAAGATTATTTTTAAAATGGACAAATTTGGACAAGGATTTTGAGTTTCCATATGACAACAATTCATAAATCAGAGGAGTCTGTATTTTAAATGTTATTCAGTTAATAAGAGCAATAGCTTTGATGAAATTAGTTAGTTAATCAATAATAGGTGTCCTGTTTTTGGCATTTTTATCCATGACATTCAGATGCTAGACAAATTTAAAGCAAATTGTAATTATCTAGTTAAATGGATTCACTTGTCCAAACAACTTACAATGTGGTCTATGATATAAATAGTGCAATGAAAATAGTCAAGTAGGAATGGAAATAATGTTTTTGCTTTAAGTGAGAGTTATATCTTCTGAATCCAATATTATGTAGCTTAAAAGCCCCACCTTCCTCTTATGGGGATGAGGTGGGGACACTGATGAATAAGATTCGTTTCTTAAGTTTGTTATGTTGTAATACTTATAGTTCTCTGATTTCTTTACATGAACAGGAGCAGAATATCAATTATGGAGTCGTGCATGAAAGGTACATACTTTCAGTTTTCATTTTTTATGTTTCTTTTGGGGTGAAATAATTGGATGTTTTATTTTTCATGAAATGGATCCTGAAAATGTAGGTACACCGGAAAGAATGGAGTTTTCTAAGTAGCTTTAAAAACCAAAGTTAGTTATTGATCACCTACTCCAAGTGCCGCAACGACCTCTTCACTTCTCCGATCTCTCAAACCCTAATTTTTTTATTTGAATTGATGAGTGTTTCATTAGGACGAAGGAGCTGTTCGATCAGTACCAGAAGACTTTAGGGCTGATCTGTGGATTACTCACTACAAGGTCTCTGATTTCCCCTTCTTTCTCCATATTTTTTAATTTTATTTTTGTAAAAGTGATTGATCTGTGATTTCCATTTTCTCTACTTTTACCTATCATGTAATACTGAGATACGTTCATAGGTGCTAATTTTTAAATTTTGTTTGGCTTTCTCTGGGAATATGAATGAACTACTAACTTAACTCTCTGGTTGTTGTATAGAATTATTGTCATTCTAGCCATCAGGCTATGTTGATAGTTGGATGGTTCAACAATTTCAGTAGCTAAAACTATGATTTAGTGAATGTTTTTTTGCAGGATGTTCAAGAGCTTGTAACTTGGGTGCTTGCTGAAGGTTTTATGCCTTCTTGGGTTTTTAATTAG

mRNA sequence

ATGCCTCATGTAAATAATCTCACTTTTCACCACAGGACAAGAAAAGAAAATTTCGCACGTGCAAGAGAACATGAAACAAATGGCAATTCTGTTGCTACCTACGAGTGCTACCAGAAGATGCTAAAAATGACCTTCTTGGTTATCAGCAATCAGGTTGATGTCGTTCTTACTGAGGACTCTGATCTAATACCGTTTGGTTGTCCAAGGGACGAAGGAGCTGTTCGATCAGTACCAGAAGACTTTAGGGCTGATCTGTGGATTACTCACTACAAGGATGTTCAAGAGCTTGTAACTTGGGTGCTTGCTGAAGGTTTTATGCCTTCTTGGGTTTTTAATTAG

Coding sequence (CDS)

ATGCCTCATGTAAATAATCTCACTTTTCACCACAGGACAAGAAAAGAAAATTTCGCACGTGCAAGAGAACATGAAACAAATGGCAATTCTGTTGCTACCTACGAGTGCTACCAGAAGATGCTAAAAATGACCTTCTTGGTTATCAGCAATCAGGTTGATGTCGTTCTTACTGAGGACTCTGATCTAATACCGTTTGGTTGTCCAAGGGACGAAGGAGCTGTTCGATCAGTACCAGAAGACTTTAGGGCTGATCTGTGGATTACTCACTACAAGGATGTTCAAGAGCTTGTAACTTGGGTGCTTGCTGAAGGTTTTATGCCTTCTTGGGTTTTTAATTAG

Protein sequence

MPHVNNLTFHHRTRKENFARAREHETNGNSVATYECYQKMLKMTFLVISNQVDVVLTEDSDLIPFGCPRDEGAVRSVPEDFRADLWITHYKDVQELVTWVLAEGFMPSWVFN

Homology

[Close]

BLAST of Lag0031294 vs. NCBI nr
Match: KAF2531092.1 (hypothetical protein F2Q70_00030550 [Brassica cretica])

HSP 1 Score: 89.4 bits (220), Expect = 2.2e-14
Identity = 43/58 (74.14%), Postives = 47/58 (81.03%), Query Frame = 0


Query: 12  RTRKENFARAREHETNGNSVATYECYQKMLKMTFLVISNQVDVVLTEDSDLIPFGCPR 70
           R+RKEN ARA EHE NGNS A YECYQK   MTFL I+ QVD ++TEDSDLIPFGCPR
Sbjct: 94  RSRKENLARALEHEANGNSSAAYECYQK--AMTFLAITKQVDAIITEDSDLIPFGCPR 149

[Close]

BLAST of Lag0031294 vs. NCBI nr
Match: XP_022954763.1 (exonuclease 1 isoform X3 [Cucurbita moschata])

HSP 1 Score: 88.6 bits (218), Expect = 3.8e-14
Identity = 49/88 (55.68%), Postives = 51/88 (57.95%), Query Frame = 0


Query: 12  RTRKENFARAREHETNGNSVATYECYQKML------------------------------ 70
           RTRKEN ARA EHETNGNS A YECYQK +                              
Sbjct: 94  RTRKENLARAMEHETNGNSAAAYECYQKAVDITPMIAYELIQVLKRENISYVVAPYEADA 153

[Close]

BLAST of Lag0031294 vs. NCBI nr
Match: XP_022994762.1 (exonuclease 1 isoform X1 [Cucurbita maxima] >XP_022994763.1 exonuclease 1 isoform X1 [Cucurbita maxima])

HSP 1 Score: 88.6 bits (218), Expect = 3.8e-14
Identity = 49/88 (55.68%), Postives = 51/88 (57.95%), Query Frame = 0


Query: 12  RTRKENFARAREHETNGNSVATYECYQKML------------------------------ 70
           RTRKEN ARA EHETNGNS A YECYQK +                              
Sbjct: 94  RTRKENLARAMEHETNGNSAAAYECYQKAVDITPMIAYELIQVLKRENISYVVAPYEADA 153

[Close]

BLAST of Lag0031294 vs. NCBI nr
Match: XP_022954760.1 (exonuclease 1 isoform X1 [Cucurbita moschata] >XP_022954761.1 exonuclease 1 isoform X1 [Cucurbita moschata])

HSP 1 Score: 88.6 bits (218), Expect = 3.8e-14
Identity = 49/88 (55.68%), Postives = 51/88 (57.95%), Query Frame = 0


Query: 12  RTRKENFARAREHETNGNSVATYECYQKML------------------------------ 70
           RTRKEN ARA EHETNGNS A YECYQK +                              
Sbjct: 94  RTRKENLARAMEHETNGNSAAAYECYQKAVDITPMIAYELIQVLKRENISYVVAPYEADA 153

[Close]

BLAST of Lag0031294 vs. NCBI nr
Match: XP_022994764.1 (exonuclease 1 isoform X2 [Cucurbita maxima])

HSP 1 Score: 88.6 bits (218), Expect = 3.8e-14
Identity = 49/88 (55.68%), Postives = 51/88 (57.95%), Query Frame = 0


Query: 12  RTRKENFARAREHETNGNSVATYECYQKML------------------------------ 70
           RTRKEN ARA EHETNGNS A YECYQK +                              
Sbjct: 94  RTRKENLARAMEHETNGNSAAAYECYQKAVDITPMIAYELIQVLKRENISYVVAPYEADA 153

[Close]

BLAST of Lag0031294 vs. ExPASy Swiss-Prot
Match: Q8L6Z7 (Exonuclease 1 OS=Arabidopsis thaliana OX=3702 GN=EXO1 PE=2 SV=2)

HSP 1 Score: 71.6 bits (174), Expect = 6.2e-12
Identity = 40/88 (45.45%), Postives = 46/88 (52.27%), Query Frame = 0


Query: 12  RTRKENFARAREHETNGNSVATYECYQKML------------------------------ 70
           R+RKEN ARA EHE NGNS A YECY K +                              
Sbjct: 94  RSRKENLARALEHEANGNSSAAYECYSKAVDISPSIAHELIQVLRQENVDYVVAPYEADA 153

[Close]

BLAST of Lag0031294 vs. ExPASy Swiss-Prot
Match: Q60GC1 (Exonuclease 1 OS=Oryza sativa subsp. japonica OX=39947 GN=EXO1 PE=2 SV=1)

HSP 1 Score: 67.8 bits (164), Expect = 9.0e-11
Identity = 39/92 (42.39%), Postives = 48/92 (52.17%), Query Frame = 0


Query: 8   TFHHRTRKENFARAREHETNGNSVATYECYQKML-------------------------- 67
           T   R+RKEN  RA+EHE+ GNS A +ECYQK +                          
Sbjct: 90  TKRERSRKENLERAKEHESAGNSRAAFECYQKAVDITPRIAFELIQVLKQEKVDYIVAPY 149

Query: 68  ----KMTFLVISNQVDVVLTEDSDLIPFGCPR 70
               +MTFL ++  VD V+TEDSDLIPFGC R
Sbjct: 150 EADAQMTFLSVNKLVDAVITEDSDLIPFGCSR 181

[Close]

BLAST of Lag0031294 vs. ExPASy Swiss-Prot
Match: Q8L7M4 (Small RNA degrading nuclease 5 OS=Arabidopsis thaliana OX=3702 GN=SDN5 PE=2 SV=2)

HSP 1 Score: 48.5 bits (114), Expect = 5.7e-05
Identity = 21/32 (65.62%), Postives = 25/32 (78.12%), Query Frame = 0


Query: 80  DFRADLWITHYKDVQELVTWVLAEGFMPSWVF 112
           DF++     + +DVQ LVTWVLAEGFMPSWVF
Sbjct: 43  DFKSPETTLNLQDVQGLVTWVLAEGFMPSWVF 74

The following BLAST results are available for this feature:

BLAST of Lag0031294 vs. NCBI nr


Match Name	E-value	Identity	Description
KAF2531092.1	2.2e-14	74.14	hypothetical protein F2Q70_00030550 [Brassica cretica]	[more]
XP_022954763.1	3.8e-14	55.68	exonuclease 1 isoform X3 [Cucurbita moschata]	[more]
XP_022994762.1	3.8e-14	55.68	exonuclease 1 isoform X1 [Cucurbita maxima] >XP_022994763.1 exonuclease 1 isofor...	[more]
XP_022954760.1	3.8e-14	55.68	exonuclease 1 isoform X1 [Cucurbita moschata] >XP_022954761.1 exonuclease 1 isof...	[more]
XP_022994764.1	3.8e-14	55.68	exonuclease 1 isoform X2 [Cucurbita maxima]	[more]

BLAST of Lag0031294 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8L6Z7	6.2e-12	45.45	Exonuclease 1 OS=Arabidopsis thaliana OX=3702 GN=EXO1 PE=2 SV=2	[more]
Q60GC1	9.0e-11	42.39	Exonuclease 1 OS=Oryza sativa subsp. japonica OX=39947 GN=EXO1 PE=2 SV=1	[more]
Q8L7M4	5.7e-05	65.63	Small RNA degrading nuclease 5 OS=Arabidopsis thaliana OX=3702 GN=SDN5 PE=2 SV=2	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.40.50.1010		coord: 8..84 e-value: 2.7E-5 score: 25.7
IPR006086	XPG-I domain	PFAM	PF00867	XPG_I	coord: 43..69 e-value: 9.0E-6 score: 26.0
IPR006084	XPG/Rad2 endonuclease	PANTHER	PTHR11081	FLAP ENDONUCLEASE FAMILY MEMBER	coord: 43..69
IPR032641	Exonuclease 1	PANTHER	PTHR11081:SF8	EXONUCLEASE 1	coord: 43..69
IPR029060	PIN-like domain superfamily	SUPERFAMILY	88723	PIN domain-like	coord: 10..69

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0031294.1	Lag0031294.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006281	DNA repair
molecular_function	GO:0035312	5'-3' exodeoxyribonuclease activity
molecular_function	GO:0004518	nuclease activity