Lag0031293 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0031293
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	Delta 9 desaturase
Location	chr11: 6721248 .. 6723467 (+)
RNA-Seq Expression	Lag0031293
Synteny	Lag0031293

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAAGCAACAAGGAAACAACTAGAAGATCATAAAGATCCTAAGAAGGTGGCATTTTCAAATGGGATTGTGATGATGAAGCCCAAACAAGCTTTCTCAAAGAGAAGATGGACAATATTGGACAAACGTTCTGCTGGTGGTCTTTTGTTCTGGCATTTGCTTTGTCTCTTGGCACCCTTTCATTGCAACTGGGCCGCGTTTTGGGTTGCCTTTGCATTGTATTTTATTACCGGTCTGTTTGGGATAACACTCTCGTACCATAGAAATCTCGCACACAGAAGCTTCAAGCTTCCCAAATGGCTCGAGTATTCGTTTGCGTATCTGGGACTGCATGCTTTTCAGGTTTGGTGAATGTTTAGATTAAATTTCTTACGATATCTTTTTGTAGTGATTTTGATTGAGTTATTCTTCTTTTTGTAACTGAAATGTTTTATTCCAACATCATAGGGAGATCCTATTGACTGGGTGAGTACACATAGGTATCATCATCAGTTTGTTGATACGGAAAGAGATCCACATAGTCCTTTGGAAGGGTTTTGGTTTAGTCATATAACTTGGATGTTCGATGCCTATACTTTGACTAAAAAAGTTTGCCCGAAAAATATCGAAGGTTTCGAGGAAAAAGATGCTAAACGAAAGCGTTTTAGTAATATTTTTGTGGCACAAGTCAAGTATGGAAGACCCAACAATGTTGAGGATTTACAAAAGCAAGCCTTTTATAGGTTTCTTCGAAGAACATACATTCTTCATCCCACTGCTCTTGCACTTGTCCTCTATGCCATAGGAGGACTTCCCTTTGTTGTTTGGGGAATGGTAACTACTATCATAGATATCTTTCATCTACTCTCTCTCTTTTTTTTTTTTTAAAGACATTAGTTTTATTTAGTAGAAGTTTCTATTGATTTTAACCTTGTGGTGTATCGATCTATTTATATTATGTTTACAATATGAATATTATTTTTTTAAGACCATGTTTTCGGACATATTTAAAACCACTTTTATCATATTCAAAATCACTATCAAACATGTTTTTAATCATTTAAAATCATGTTTTATTTAGCAAAAGTTTCTACTGATTTTAACGTGGTTGTCTATCGAACTATTTATATTATGTTTACAATATGAATATTACTTTTTAAGACTATGTTTTCCGGCATGTTTAAAACCACTTTTATCATATTCAAAATCACTATCAAACATGCTTTTAATCATTTAATAAAATCAATTTTCTTTTAGTTCATTTAAAATCAATTTTTATTATATGAAAATTGGGTTTAAAAGTATTAAACTAAACACTAAAATGATTATATGAAAGTCACTTTAAAGTCCATTTGGAATGTCTTGTCAAATGCATAAAAATATTTTTTTTCACTTAAAAATACTTCTAAAATCACTTAAACACATCACTTATAAACAAATCATTTTCACTAAAATCAATATTTCTAAAATCACTCCTCCTTAGTTTGAGTGTGATCATGATCCCAACACGGAACAACATATTTATTTAAACTTTTATTTTTTAAAAACTCTTAATACTTGGCTGCAGATTCGTACACACTCCATTAAAATCTTGACAACTAAAAGAGAAAGGTAAAAAGTGAGAAACTTATTTTACCTCTCTTAATTGTAGACATTTTAATGGTGGTATGCAGAAACACCAGTAGGGATATTAGAAATAAAATATTATATCATTAAATGACGTGTTAAATGTTGAATGGACTATGTATTACGGTACTACTAGAAATTTTCCGAGATATAGTCCCAATGAAGGTTTGATAGTGATGGCTTGCATGACAGGGGGTGAGGCTTGTTTGGTTTTTGCACATTACTTTCTTCGTAAATTCGGCATGCCACATATGGGGATACCGACTATGGAACACGGCCGATCTGTCCAAGAATAATTGGTAGTGCTGTCTCTTTCTCATGTTTCTGTTTCAGATTTCTTTTTCTTTTTTTTAAGGTTGATGTTTTTCTTAAACAACATATTGATTAAATGATATTGCTGTTCGCAGGCTGTTCGGTTTACTTGCGTTTGGGGAGGGTTGGCACAACAATCACCATGCATTTGAATATTCAGCTCGCCATGGTCTTCAATGGTGGGAGTTTGACTTGACATGGTGGGTTGTCTTGTTCCTTCAAGCTGTTGGATTGGCGACCGACGTCAAATTACCTTCTAAACTTCACAAGCAAAAGTTGGCATTGGACCATGAAAAAGTTTGA

mRNA sequence

ATGGAAGCAACAAGGAAACAACTAGAAGATCATAAAGATCCTAAGAAGGTGGCATTTTCAAATGGGATTGTGATGATGAAGCCCAAACAAGCTTTCTCAAAGAGAAGATGGACAATATTGGACAAACGTTCTGCTGGTGGTCTTTTGTTCTGGCATTTGCTTTGTCTCTTGGCACCCTTTCATTGCAACTGGGCCGCGTTTTGGGTTGCCTTTGCATTGTATTTTATTACCGGTCTGTTTGGGATAACACTCTCGTACCATAGAAATCTCGCACACAGAAGCTTCAAGCTTCCCAAATGGCTCGAGTATTCGTTTGCGTATCTGGGACTGCATGCTTTTCAGGGAGATCCTATTGACTGGGTGAGTACACATAGGTATCATCATCAGTTTGTTGATACGGAAAGAGATCCACATAGTCCTTTGGAAGGGTTTTGGTTTAGTCATATAACTTGGATGTTCGATGCCTATACTTTGACTAAAAAAGTTTGCCCGAAAAATATCGAAGGTTTCGAGGAAAAAGATGCTAAACGAAAGCGTTTTAGTAATATTTTTGTGGCACAAGTCAAGTATGGAAGACCCAACAATGTTGAGGATTTACAAAAGCAAGCCTTTTATAGGTTTCTTCGAAGAACATACATTCTTCATCCCACTGCTCTTGCACTTGTCCTCTATGCCATAGGAGGACTTCCCTTTGTTGTTTGGGGAATGGGGGTGAGGCTTGTTTGGTTTTTGCACATTACTTTCTTCGTAAATTCGGCATGCCACATATGGGGATACCGACTATGGAACACGGCCGATCTGTCCAAGAATAATTGGCTGTTCGGTTTACTTGCGTTTGGGGAGGGTTGGCACAACAATCACCATGCATTTGAATATTCAGCTCGCCATGGTCTTCAATGGTGGGAGTTTGACTTGACATGGTGGGTTGTCTTGTTCCTTCAAGCTGTTGGATTGGCGACCGACGTCAAATTACCTTCTAAACTTCACAAGCAAAAGTTGGCATTGGACCATGAAAAAGTTTGA

Coding sequence (CDS)

ATGGAAGCAACAAGGAAACAACTAGAAGATCATAAAGATCCTAAGAAGGTGGCATTTTCAAATGGGATTGTGATGATGAAGCCCAAACAAGCTTTCTCAAAGAGAAGATGGACAATATTGGACAAACGTTCTGCTGGTGGTCTTTTGTTCTGGCATTTGCTTTGTCTCTTGGCACCCTTTCATTGCAACTGGGCCGCGTTTTGGGTTGCCTTTGCATTGTATTTTATTACCGGTCTGTTTGGGATAACACTCTCGTACCATAGAAATCTCGCACACAGAAGCTTCAAGCTTCCCAAATGGCTCGAGTATTCGTTTGCGTATCTGGGACTGCATGCTTTTCAGGGAGATCCTATTGACTGGGTGAGTACACATAGGTATCATCATCAGTTTGTTGATACGGAAAGAGATCCACATAGTCCTTTGGAAGGGTTTTGGTTTAGTCATATAACTTGGATGTTCGATGCCTATACTTTGACTAAAAAAGTTTGCCCGAAAAATATCGAAGGTTTCGAGGAAAAAGATGCTAAACGAAAGCGTTTTAGTAATATTTTTGTGGCACAAGTCAAGTATGGAAGACCCAACAATGTTGAGGATTTACAAAAGCAAGCCTTTTATAGGTTTCTTCGAAGAACATACATTCTTCATCCCACTGCTCTTGCACTTGTCCTCTATGCCATAGGAGGACTTCCCTTTGTTGTTTGGGGAATGGGGGTGAGGCTTGTTTGGTTTTTGCACATTACTTTCTTCGTAAATTCGGCATGCCACATATGGGGATACCGACTATGGAACACGGCCGATCTGTCCAAGAATAATTGGCTGTTCGGTTTACTTGCGTTTGGGGAGGGTTGGCACAACAATCACCATGCATTTGAATATTCAGCTCGCCATGGTCTTCAATGGTGGGAGTTTGACTTGACATGGTGGGTTGTCTTGTTCCTTCAAGCTGTTGGATTGGCGACCGACGTCAAATTACCTTCTAAACTTCACAAGCAAAAGTTGGCATTGGACCATGAAAAAGTTTGA

Protein sequence

MEATRKQLEDHKDPKKVAFSNGIVMMKPKQAFSKRRWTILDKRSAGGLLFWHLLCLLAPFHCNWAAFWVAFALYFITGLFGITLSYHRNLAHRSFKLPKWLEYSFAYLGLHAFQGDPIDWVSTHRYHHQFVDTERDPHSPLEGFWFSHITWMFDAYTLTKKVCPKNIEGFEEKDAKRKRFSNIFVAQVKYGRPNNVEDLQKQAFYRFLRRTYILHPTALALVLYAIGGLPFVVWGMGVRLVWFLHITFFVNSACHIWGYRLWNTADLSKNNWLFGLLAFGEGWHNNHHAFEYSARHGLQWWEFDLTWWVVLFLQAVGLATDVKLPSKLHKQKLALDHEKV

Homology

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BLAST of Lag0031293 vs. NCBI nr
Match: XP_022153442.1 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [Momordica charantia])

HSP 1 Score: 662.5 bits (1708), Expect = 1.9e-186
Identity = 304/340 (89.41%), Postives = 325/340 (95.59%), Query Frame = 0


Query: 1   MEATRKQLEDHKDPKKVAFSNGIVMMKPKQAFSKRRWTILDKRSAGGLLFWHLLCLLAPF 60
           MEATRK+LED  + KKV FS+GIV +KP++ F KR+WTILD+RSAGG+LF HLLCLLAPF
Sbjct: 1   MEATRKELEDKNEXKKVGFSDGIV-LKPRRGFLKRKWTILDRRSAGGILFLHLLCLLAPF 60

Query: 61  HCNWAAFWVAFALYFITGLFGITLSYHRNLAHRSFKLPKWLEYSFAYLGLHAFQGDPIDW 120
           HCNWAAFWVAFALYFITGLFGITLSYHRNLAH+SFKLPKWLEY FAYLGLHAFQGDPIDW
Sbjct: 61  HCNWAAFWVAFALYFITGLFGITLSYHRNLAHKSFKLPKWLEYLFAYLGLHAFQGDPIDW 120

Query: 121 VSTHRYHHQFVDTERDPHSPLEGFWFSHITWMFDAYTLTKKVCPKNIEGFEEKDAKRKRF 180
           VSTHRYHHQFVDTERDPHSPLEGFWFSHITWMFDAYTLTKKVCPKN+E FEEKDAKRKRF
Sbjct: 121 VSTHRYHHQFVDTERDPHSPLEGFWFSHITWMFDAYTLTKKVCPKNVEDFEEKDAKRKRF 180

Query: 181 SNIFVAQVKYGRPNNVEDLQKQAFYRFLRRTYILHPTALALVLYAIGGLPFVVWGMGVRL 240
           SNIFVAQVKYGRPNNV+DLQKQAFYRFLRRTYILHPTALALVLYA+GGLPFVVWGMGVRL
Sbjct: 181 SNIFVAQVKYGRPNNVDDLQKQAFYRFLRRTYILHPTALALVLYAMGGLPFVVWGMGVRL 240

Query: 241 VWFLHITFFVNSACHIWGYRLWNTADLSKNNWLFGLLAFGEGWHNNHHAFEYSARHGLQW 300
           VWFLHITFF+NSACHIWGYR WNT DLSKNNW+FGLLAFGEGWHNNHHAFEYSARHGLQW
Sbjct: 241 VWFLHITFFINSACHIWGYRRWNTTDLSKNNWVFGLLAFGEGWHNNHHAFEYSARHGLQW 300

Query: 301 WEFDLTWWVVLFLQAVGLATDVKLPSKLHKQKLALDHEKV 341
           WEFDLTWWVVLFLQ +GLATDVKLPS+  KQKLA+DH+++
Sbjct: 301 WEFDLTWWVVLFLQTIGLATDVKLPSQGQKQKLAMDHKRI 339

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BLAST of Lag0031293 vs. NCBI nr
Match: XP_022153467.1 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [Momordica charantia])

HSP 1 Score: 610.9 bits (1574), Expect = 6.6e-171
Identity = 285/335 (85.07%), Postives = 310/335 (92.54%), Query Frame = 0


Query: 1   MEATRKQLEDHKDPKKVAFSNGIVMMKPKQAFSKRRWTILDKRSAGGLLFWHLLCLLAPF 60
           MEATRK++ED KD KKVAFS    ++KP++   KR+WTILD+RSAG +LF HLL LLAPF
Sbjct: 1   MEATRKEVEDQKD-KKVAFS----VVKPRRPLLKRKWTILDRRSAGAILFLHLLSLLAPF 60

Query: 61  HCNWAAFWVAFALYFITGLFGITLSYHRNLAHRSFKLPKWLEYSFAYLGLHAFQGDPIDW 120
           HCNWAAFWVAFALYFITGLFGITLSYHRNLAH+SFKLPKWLEY+FAYLG+HAFQGDPIDW
Sbjct: 61  HCNWAAFWVAFALYFITGLFGITLSYHRNLAHKSFKLPKWLEYAFAYLGVHAFQGDPIDW 120

Query: 121 VSTHRYHHQFVDTERDPHSPLEGFWFSHITWMFDAYTLTKKVCPKNIEGFEEKDAKRKRF 180
           VSTHRYHH+FVDTERDPHSPLEGFWFSHI WMFDAY LT+KVCPKN++ F+E+DAKRKRF
Sbjct: 121 VSTHRYHHRFVDTERDPHSPLEGFWFSHIIWMFDAYALTEKVCPKNVQDFKERDAKRKRF 180

Query: 181 SNIFVAQVKYGRPNNVEDLQKQAFYRFLRRTYILHPTALALVLYAIGGLPFVVWGMGVRL 240
           SNIFV  VKYGRPNNVEDLQKQAFYRFLR+TYILHPTALA+VLYA GGLPF+VWGMGVRL
Sbjct: 181 SNIFVGFVKYGRPNNVEDLQKQAFYRFLRKTYILHPTALAIVLYATGGLPFLVWGMGVRL 240

Query: 241 VWFLHITFFVNSACHIWGYRLWNTADLSKNNWLFGLLAFGEGWHNNHHAFEYSARHGLQW 300
           VW LHITFFVNSACHIWGYR W+T DLSKNNW  GLLAFGEGWHNNHHAFEYSARHGLQW
Sbjct: 241 VWVLHITFFVNSACHIWGYRRWDTNDLSKNNWWIGLLAFGEGWHNNHHAFEYSARHGLQW 300

Query: 301 WEFDLTWWVVLFLQAVGLATDVKLPSKLHKQKLAL 336
           WEFDLTW VVL LQA+GLATDVKLPS++HKQKLAL
Sbjct: 301 WEFDLTWCVVLVLQALGLATDVKLPSQIHKQKLAL 330

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BLAST of Lag0031293 vs. NCBI nr
Match: KAG6606803.1 (Palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 583.2 bits (1502), Expect = 1.5e-162
Identity = 266/325 (81.85%), Postives = 291/325 (89.54%), Query Frame = 0


Query: 12  KDPKKVAFSNGIVMMKPKQAFSKRRWTILDKRSAGGLLFWHLLCLLAPFHCNWAAFWVAF 71
           ++ KKVA S    M+K K A   R+WTILD+RSAGG+LF H LCLLAPFHCNW AFWVAF
Sbjct: 2   RNTKKVALS----MVKSKPALLNRKWTILDRRSAGGVLFLHFLCLLAPFHCNWPAFWVAF 61

Query: 72  ALYFITGLFGITLSYHRNLAHRSFKLPKWLEYSFAYLGLHAFQGDPIDWVSTHRYHHQFV 131
           ALYFITGLFG+TLSYHRNLAH+SFKLPKWLEY FAYLG+ AFQGDPIDWVSTHRYHHQFV
Sbjct: 62  ALYFITGLFGVTLSYHRNLAHKSFKLPKWLEYLFAYLGVQAFQGDPIDWVSTHRYHHQFV 121

Query: 132 DTERDPHSPLEGFWFSHITWMFDAYTLTKKVCPKNIEGFEEKDAKRKRFSNIFVAQVKYG 191
           DT+RDPHSPL+GFWFSH+ WMFDAY LTKKVCPKN++ FEE+  KRKRF N+ V  VKYG
Sbjct: 122 DTDRDPHSPLDGFWFSHLAWMFDAYALTKKVCPKNVQDFEERSTKRKRFRNVLVGYVKYG 181

Query: 192 RPNNVEDLQKQAFYRFLRRTYILHPTALALVLYAIGGLPFVVWGMGVRLVWFLHITFFVN 251
           RPNNVEDLQKQ FYRFLR+TYILHPTALA+VLYA GGLPFVVWGMGVRLVW LHITFFVN
Sbjct: 182 RPNNVEDLQKQGFYRFLRKTYILHPTALAIVLYATGGLPFVVWGMGVRLVWVLHITFFVN 241

Query: 252 SACHIWGYRLWNTADLSKNNWLFGLLAFGEGWHNNHHAFEYSARHGLQWWEFDLTWWVVL 311
           SACHIWGYRLW+T DLSKNNW+ GLLAFGEGWHNNHHAFEYSARHG +WWEFDLTW V+L
Sbjct: 242 SACHIWGYRLWDTNDLSKNNWVIGLLAFGEGWHNNHHAFEYSARHGYEWWEFDLTWCVIL 301

Query: 312 FLQAVGLATDVKLPSKLHKQKLALD 337
           FLQA+GLATDVKLPS  H+QKLAL+
Sbjct: 302 FLQALGLATDVKLPSARHQQKLALN 322

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BLAST of Lag0031293 vs. NCBI nr
Match: XP_023525371.1 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 578.9 bits (1491), Expect = 2.8e-161
Identity = 264/325 (81.23%), Postives = 291/325 (89.54%), Query Frame = 0


Query: 12  KDPKKVAFSNGIVMMKPKQAFSKRRWTILDKRSAGGLLFWHLLCLLAPFHCNWAAFWVAF 71
           ++ KKVAFS    M+K K +   R+WTILD+RSAGG+LF H L LLAPFHCNW AFWVAF
Sbjct: 2   RNTKKVAFS----MVKSKPSLLNRKWTILDRRSAGGVLFLHFLSLLAPFHCNWPAFWVAF 61

Query: 72  ALYFITGLFGITLSYHRNLAHRSFKLPKWLEYSFAYLGLHAFQGDPIDWVSTHRYHHQFV 131
           ALYFITGLFG+TLSYHRNLAH+SFKLPKW EY FAYLG+ AFQGDPIDWVSTHRYHHQFV
Sbjct: 62  ALYFITGLFGVTLSYHRNLAHKSFKLPKWFEYLFAYLGVQAFQGDPIDWVSTHRYHHQFV 121

Query: 132 DTERDPHSPLEGFWFSHITWMFDAYTLTKKVCPKNIEGFEEKDAKRKRFSNIFVAQVKYG 191
           DT+RDPHSPL+GFWFSH+ WMFDAYTLTKKVCPKN++ FEE+  KRKRF N+ V  VKYG
Sbjct: 122 DTDRDPHSPLDGFWFSHLAWMFDAYTLTKKVCPKNVQDFEERSTKRKRFRNVLVGYVKYG 181

Query: 192 RPNNVEDLQKQAFYRFLRRTYILHPTALALVLYAIGGLPFVVWGMGVRLVWFLHITFFVN 251
           RPNNVEDLQKQ FYRFLR+TYILHPTALA+VLYA GGLPFVVWGMGVRLVW LHITFFVN
Sbjct: 182 RPNNVEDLQKQGFYRFLRKTYILHPTALAIVLYATGGLPFVVWGMGVRLVWVLHITFFVN 241

Query: 252 SACHIWGYRLWNTADLSKNNWLFGLLAFGEGWHNNHHAFEYSARHGLQWWEFDLTWWVVL 311
           SACHIWGYRLW+T DLSKNNW+ GLLAFGEGWHNNHHAFEYSARHG +WWEFDLTW V+L
Sbjct: 242 SACHIWGYRLWDTNDLSKNNWVIGLLAFGEGWHNNHHAFEYSARHGYEWWEFDLTWCVIL 301

Query: 312 FLQAVGLATDVKLPSKLHKQKLALD 337
           FLQA+GLAT+VKLPS  H+QKLAL+
Sbjct: 302 FLQALGLATNVKLPSARHQQKLALN 322

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BLAST of Lag0031293 vs. NCBI nr
Match: XP_022949581.1 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 578.2 bits (1489), Expect = 4.7e-161
Identity = 265/325 (81.54%), Postives = 290/325 (89.23%), Query Frame = 0


Query: 12  KDPKKVAFSNGIVMMKPKQAFSKRRWTILDKRSAGGLLFWHLLCLLAPFHCNWAAFWVAF 71
           ++ KKVA S    M+K K A   R+WTILD+RSAGG+LF H LCLLAPFHCNW AFWVAF
Sbjct: 2   RNTKKVALS----MVKSKPALLNRKWTILDRRSAGGVLFLHFLCLLAPFHCNWPAFWVAF 61

Query: 72  ALYFITGLFGITLSYHRNLAHRSFKLPKWLEYSFAYLGLHAFQGDPIDWVSTHRYHHQFV 131
           ALYFITGLFG+TLSYHRNLAH+SFKLPKWLEY FAYLG+ AFQGDPIDWVSTHRYHHQFV
Sbjct: 62  ALYFITGLFGVTLSYHRNLAHKSFKLPKWLEYLFAYLGVQAFQGDPIDWVSTHRYHHQFV 121

Query: 132 DTERDPHSPLEGFWFSHITWMFDAYTLTKKVCPKNIEGFEEKDAKRKRFSNIFVAQVKYG 191
           DT+RDPHSPL+GFWFSH+ WMFDAYTLTKKV PKN++ FEE+  KRKRF N+ V  VKYG
Sbjct: 122 DTDRDPHSPLDGFWFSHLAWMFDAYTLTKKVYPKNVQDFEERSTKRKRFRNVLVGYVKYG 181

Query: 192 RPNNVEDLQKQAFYRFLRRTYILHPTALALVLYAIGGLPFVVWGMGVRLVWFLHITFFVN 251
           RPNNVEDLQKQ FYRFLR+TYILHPTA A+VLYA GGLPFVVWGMGVRLVW LHITFFVN
Sbjct: 182 RPNNVEDLQKQGFYRFLRKTYILHPTAPAIVLYATGGLPFVVWGMGVRLVWVLHITFFVN 241

Query: 252 SACHIWGYRLWNTADLSKNNWLFGLLAFGEGWHNNHHAFEYSARHGLQWWEFDLTWWVVL 311
           SACHIWGYRLW+T DLSKNNW+ GLLAFGEGWHNNHHAFEYSARHG +WWEFDLTW V+L
Sbjct: 242 SACHIWGYRLWDTNDLSKNNWVIGLLAFGEGWHNNHHAFEYSARHGYEWWEFDLTWCVIL 301

Query: 312 FLQAVGLATDVKLPSKLHKQKLALD 337
           FLQA+GLATDVKLPS  H+QKLAL+
Sbjct: 302 FLQALGLATDVKLPSARHQQKLALN 322

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BLAST of Lag0031293 vs. ExPASy Swiss-Prot
Match: Q949X0 (Palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ADS3 PE=1 SV=2)

HSP 1 Score: 421.4 bits (1082), Expect = 9.7e-117
Identity = 194/322 (60.25%), Postives = 234/322 (72.67%), Query Frame = 0


Query: 13  DPKKVAFSNGIVMMKPKQAFSKRRWTILDKRSAGGLLFWHLLCLLAPFHCNWAAFWVAFA 72
           D +++  S+ +V  K K  + +R W  +D  +   +L  HLL LLAPF  NW A  VAF 
Sbjct: 74  DYRRIMLSDVLVKKKEKVVWWEREWKAMDFGAVAVVLSMHLLSLLAPFQFNWRAVSVAFG 133

Query: 73  LYFITGLFGITLSYHRNLAHRSFKLPKWLEYSFAYLGLHAFQGDPIDWVSTHRYHHQFVD 132
           LY +TGL GITLS+HRNL+H++FKLPKWLEY FAY G  A QG+PIDWVSTHRYHHQF D
Sbjct: 134 LYIVTGLLGITLSFHRNLSHKAFKLPKWLEYLFAYCGAQALQGNPIDWVSTHRYHHQFCD 193

Query: 133 TERDPHSPLEGFWFSHITWMFDAYTLTKKVCPKNIEGFEEKDAKRKRFSNIFVAQVKYGR 192
           ++RDPHSPL+GFWFSH+ WMFD  T+T++                             G 
Sbjct: 194 SDRDPHSPLDGFWFSHMNWMFDTNTITQRC----------------------------GE 253

Query: 193 PNNVEDLQKQAFYRFLRRTYILHPTALALVLYAIGGLPFVVWGMGVRLVWFLHITFFVNS 252
           PNNV DL+KQ FYRFLR TYILHP ALA+ LYA+GG PF+VWGMGVR+VW  HIT+ VNS
Sbjct: 254 PNNVGDLEKQPFYRFLRTTYILHPLALAVALYAMGGFPFIVWGMGVRIVWVYHITWLVNS 313

Query: 253 ACHIWGYRLWNTADLSKNNWLFGLLAFGEGWHNNHHAFEYSARHGLQWWEFDLTWWVVLF 312
           ACH+WG + WNT DLSKNNW    LAFGEGWHNNHHAFE+SARHGL+WW+ D+TW+VV F
Sbjct: 314 ACHVWGKQAWNTGDLSKNNWWVAALAFGEGWHNNHHAFEFSARHGLEWWQLDMTWYVVKF 367

Query: 313 LQAVGLATDVKLPSKLHKQKLA 335
           LQA+GLATDVKLPS+  KQ++A
Sbjct: 374 LQAIGLATDVKLPSEAQKQRMA 367

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BLAST of Lag0031293 vs. ExPASy Swiss-Prot
Match: P0DOW3 (Acyl-CoA 5-desaturase AL21 OS=Anemone leveillei OX=212809 GN=AL21 PE=1 SV=1)

HSP 1 Score: 354.0 bits (907), Expect = 1.9e-96
Identity = 170/337 (50.45%), Postives = 216/337 (64.09%), Query Frame = 0


Query: 1   MEATRKQLEDHKDPKKVAFSNGIVMMKPKQAFSKRRWTILDKRS---AGGLLFWHLLCLL 60
           ME     L+      K   S+  V  K K+    R W   D       GGL   H+LCL 
Sbjct: 1   MELPAMALQSELKQDKFPSSDVPVKDKTKKITWVRVWNSTDIFHIILVGGL---HVLCLS 60

Query: 61  APFHCNWAAFWVAFALYFITGLFGITLSYHRNLAHRSFKLPKWLEYSFAYLGLHAFQGDP 120
           APF  +W+AFW++  LY + G+FG TLSYHRNL HRSFKLPK+LEY FAY+GLHA QGDP
Sbjct: 61  APFTFSWSAFWLSLTLYAVCGVFGTTLSYHRNLTHRSFKLPKYLEYFFAYVGLHALQGDP 120

Query: 121 IDWVSTHRYHHQFVDTERDPHSPLEGFWFSHITWMFDAYTLTKKVCPKNIEGFEEKDAKR 180
           + WVSTHRYHH+F DT  DPHSP+EGFWFSHI W+FD+  + ++                
Sbjct: 121 VWWVSTHRYHHKFTDTYLDPHSPIEGFWFSHIFWLFDSKYILEEC--------------- 180

Query: 181 KRFSNIFVAQVKYGRPNNVEDLQKQAFYRFLRRTYILHPTALALVLYAIGGLPFVVWGMG 240
                        GR  N  DL KQ++YRFL RT++ H    A +LY  GG PF+VWGM 
Sbjct: 181 -------------GRYENAGDLLKQSYYRFLERTFVFHVYLQAALLYMFGGFPFIVWGMA 240

Query: 241 VRLVWFLHITFFVNSACHIWGYRLWNTADLSKNNWLFGLLAFGEGWHNNHHAFEYSARHG 300
           VR VW  H ++ VNS CH +G++ W+T DLS NNW   +L  GEGWHNNHHAFEYSARHG
Sbjct: 241 VRTVWGFHASWLVNSVCHRYGHQAWDTGDLSTNNWFIAMLTSGEGWHNNHHAFEYSARHG 300

Query: 301 LQWWEFDLTWWVVLFLQAVGLATDVKLPSKLHKQKLA 335
           ++WW+ D TW+V+  L+ +GLATDVK+PS++HK+K++
Sbjct: 301 IEWWQIDTTWYVIKLLEYLGLATDVKVPSEVHKRKMS 306

[Close]

BLAST of Lag0031293 vs. ExPASy Swiss-Prot
Match: P0DOW2 (Acyl-CoA C20 Delta5-desaturase OS=Anemone leveillei OX=212809 GN=AL10 PE=1 SV=1)

HSP 1 Score: 352.4 bits (903), Expect = 5.6e-96
Identity = 164/314 (52.23%), Postives = 207/314 (65.92%), Query Frame = 0


Query: 24  VMMKPKQAFSKRRWTILDK---RSAGGLLFWHLLCLLAPFHCNWAAFWVAFALYFITGLF 83
           V  K K+    R+W   D       GGL   H+LCL AP   +W +FWV FALY I G+F
Sbjct: 27  VKEKTKKITWVRKWNSTDVFHILFIGGL---HVLCLFAPSTFSWKSFWVCFALYAICGVF 86

Query: 84  GITLSYHRNLAHRSFKLPKWLEYSFAYLGLHAFQGDPIDWVSTHRYHHQFVDTERDPHSP 143
           G TLS+HRNL HRSFKLPK+LEY FAY+GLHA QGDP+ WVSTHRYHH++ DT  DPHSP
Sbjct: 87  GTTLSFHRNLTHRSFKLPKYLEYFFAYVGLHALQGDPVWWVSTHRYHHKYTDTYLDPHSP 146

Query: 144 LEGFWFSHITWMFDAYTLTKKVCPKNIEGFEEKDAKRKRFSNIFVAQVKYGRPNNVEDLQ 203
           +EGFWF HI W+FD+  + +K                             GR  N  DL 
Sbjct: 147 IEGFWFCHIFWLFDSKYIIEKC----------------------------GRYENAGDLM 206

Query: 204 KQAFYRFLRRTYILHPTALALVLYAIGGLPFVVWGMGVRLVWFLHITFFVNSACHIWGYR 263
           KQ++YRFL RT++ H    A +LY  GG PF+VWGM VR +   H+++ VNS CH WG R
Sbjct: 207 KQSYYRFLERTFVYHVYLQAALLYLFGGFPFIVWGMAVRTILGFHLSWLVNSVCHRWGNR 266

Query: 264 LWNTADLSKNNWLFGLLAFGEGWHNNHHAFEYSARHGLQWWEFDLTWWVVLFLQAVGLAT 323
            WNT DLS NNW   +L  GEGWHNNHHAFEYSARHG++WW+ D TW+++  L+ +GLAT
Sbjct: 267 PWNTGDLSTNNWFIAMLTSGEGWHNNHHAFEYSARHGIEWWQIDTTWYIIKLLEYLGLAT 309

Query: 324 DVKLPSKLHKQKLA 335
           D+K+PS++HK+K++
Sbjct: 327 DIKVPSEIHKRKMS 309

[Close]

BLAST of Lag0031293 vs. ExPASy Swiss-Prot
Match: Q9FV68 (Icosanoyl-CoA 5-desaturase (Fragment) OS=Limnanthes douglasii OX=28973 PE=1 SV=1)

HSP 1 Score: 349.4 bits (895), Expect = 4.7e-95
Identity = 170/323 (52.63%), Postives = 216/323 (66.87%), Query Frame = 0


Query: 12  KDPKKVAFSNGIVMMKPKQAFSKRRWTILDKRSAGGLLFWHLLCLLAPFHCNWAAFWVAF 71
           KD  +   ++ +V+ +  + F +R+W   D ++A  LL  H L  +APF+ +W AFW++F
Sbjct: 56  KDDLERFRTSEVVLERKAKGFWRRKWNPRDIQNAVTLLVLHALAAMAPFYFSWDAFWISF 115

Query: 72  ALY-FITGLFGITLSYHRNLAHRSFKLPKWLEYSFAYLGLHAFQGDPIDWVSTHRYHHQF 131
            L  F +G+ GITL +HR L H  FKLPK +EY FAY G  A QGDP++WVS HRYHHQF
Sbjct: 116 ILLGFASGVLGITLCFHRCLTHGGFKLPKLVEYFFAYCGSLALQGDPMEWVSNHRYHHQF 175

Query: 132 VDTERDPHSPLEGFWFSHITWMFDAYTLTKKVCPKNIEGFEEKDAKRKRFSNIFVAQVKY 191
           VDTERD HSP +GFWF HI W+ D                           ++FV + + 
Sbjct: 176 VDTERDVHSPTQGFWFCHIGWVLD--------------------------KDLFV-EKRG 235

Query: 192 GRPNNVEDLQKQAFYRFLRRTYILHPTALALVLYAIGGLPFVVWGMGVRLVWFLHITFFV 251
           GR NNV DL+KQAFYRFL++TY+ H  AL  +LY +GG P++VWGMG RLV+  H TF +
Sbjct: 236 GRRNNVNDLKKQAFYRFLQKTYMYHQLALIALLYYVGGFPYIVWGMGFRLVFMFHSTFAI 295

Query: 252 NSACHIWGYRLWNTADLSKNNWLFGLLAFGEGWHNNHHAFEYSARHGLQWWEFDLTWWVV 311
           NS CH WG R WNT DLS NN    L AFGEGWHNNHHAFE SARHGL+WWE D+TW+V+
Sbjct: 296 NSVCHKWGGRPWNTGDLSTNNMFVALCAFGEGWHNNHHAFEQSARHGLEWWEIDVTWYVI 351

Query: 312 LFLQAVGLATDVKLPSKLHKQKL 334
             LQA+GLAT+VKLP++  KQKL
Sbjct: 356 RTLQAIGLATNVKLPTEAQKQKL 351

[Close]

BLAST of Lag0031293 vs. ExPASy Swiss-Prot
Match: Q9LVZ3 (Probable lipid desaturase ADS3.2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ADS3.2 PE=2 SV=3)

HSP 1 Score: 346.7 bits (888), Expect = 3.0e-94
Identity = 164/326 (50.31%), Postives = 215/326 (65.95%), Query Frame = 0


Query: 10  DHKDPKKVAFSNGIVMMKPKQAFSKRRWTILDKRSAGGLLFWHLLCLLAPFHCNWAAFWV 69
           DH +       + +V+++ K++F +R WT  D       +  HLL LLAPF+ +W AFWV
Sbjct: 64  DHVESSWRRLLSEVVVVRTKRSFWERSWTSWDVSKLVIFVGTHLLSLLAPFYFSWEAFWV 123

Query: 70  AFALYFITGLFGITLSYHRNLAHRSFKLPKWLEYSFAYLGLHAFQGDPIDWVSTHRYHHQ 129
              L FI G+  ITLSYHRNL+HRSF LPKWLEY FAY G+ AFQGDPI+WVS HRYHH+
Sbjct: 124 FPWLVFINGIC-ITLSYHRNLSHRSFDLPKWLEYLFAYGGVLAFQGDPIEWVSNHRYHHK 183

Query: 130 FVDTERDPHSPLEGFWFSHITWMFDAYTLTKKVCPKNIEGFEEKDAKRKRFSNIFVAQVK 189
             +T+RDPHSP +GFWFSH+ W+FD  ++ +                             
Sbjct: 184 HCETQRDPHSPTQGFWFSHMAWIFDTSSILENC--------------------------- 243

Query: 190 YGRPNNVEDLQKQAFYRFLRRTYILHPTALALVLYAIGGLPFVVWGMGVRLVWFLHITFF 249
            G   NV+DL +Q FYRFL+RT +LH  A + + Y  GG+P +VWG+G+ +   LH+TF 
Sbjct: 244 -GGEENVDDLVRQPFYRFLQRTVLLHMMAYSFLFYFCGGMPLLVWGIGITIAVRLHLTFL 303

Query: 250 VNSACHIWGYRLWNTADLSKNNWLFGLLAFGEGWHNNHHAFEYSARHGLQWWEFDLTWWV 309
           VNS CHIWG R WNT+D SKNNW   +L  GEGWHNNHHAFE+SARHGL+WW+ D+TW +
Sbjct: 304 VNSVCHIWGTRAWNTSDFSKNNWWVAILTLGEGWHNNHHAFEFSARHGLEWWQLDITWCL 360

Query: 310 VLFLQAVGLATDVKLPSKLHKQKLAL 336
           + FL+A+GLAT+VKLP++   +  AL
Sbjct: 364 IRFLEAIGLATNVKLPTETQMKGKAL 360

[Close]

BLAST of Lag0031293 vs. ExPASy TrEMBL
Match: A0A6J1DIX0 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS=Momordica charantia OX=3673 GN=LOC111020949 PE=3 SV=1)

HSP 1 Score: 662.5 bits (1708), Expect = 9.3e-187
Identity = 304/340 (89.41%), Postives = 325/340 (95.59%), Query Frame = 0


Query: 1   MEATRKQLEDHKDPKKVAFSNGIVMMKPKQAFSKRRWTILDKRSAGGLLFWHLLCLLAPF 60
           MEATRK+LED  + KKV FS+GIV +KP++ F KR+WTILD+RSAGG+LF HLLCLLAPF
Sbjct: 1   MEATRKELEDKNEXKKVGFSDGIV-LKPRRGFLKRKWTILDRRSAGGILFLHLLCLLAPF 60

Query: 61  HCNWAAFWVAFALYFITGLFGITLSYHRNLAHRSFKLPKWLEYSFAYLGLHAFQGDPIDW 120
           HCNWAAFWVAFALYFITGLFGITLSYHRNLAH+SFKLPKWLEY FAYLGLHAFQGDPIDW
Sbjct: 61  HCNWAAFWVAFALYFITGLFGITLSYHRNLAHKSFKLPKWLEYLFAYLGLHAFQGDPIDW 120

Query: 121 VSTHRYHHQFVDTERDPHSPLEGFWFSHITWMFDAYTLTKKVCPKNIEGFEEKDAKRKRF 180
           VSTHRYHHQFVDTERDPHSPLEGFWFSHITWMFDAYTLTKKVCPKN+E FEEKDAKRKRF
Sbjct: 121 VSTHRYHHQFVDTERDPHSPLEGFWFSHITWMFDAYTLTKKVCPKNVEDFEEKDAKRKRF 180

Query: 181 SNIFVAQVKYGRPNNVEDLQKQAFYRFLRRTYILHPTALALVLYAIGGLPFVVWGMGVRL 240
           SNIFVAQVKYGRPNNV+DLQKQAFYRFLRRTYILHPTALALVLYA+GGLPFVVWGMGVRL
Sbjct: 181 SNIFVAQVKYGRPNNVDDLQKQAFYRFLRRTYILHPTALALVLYAMGGLPFVVWGMGVRL 240

Query: 241 VWFLHITFFVNSACHIWGYRLWNTADLSKNNWLFGLLAFGEGWHNNHHAFEYSARHGLQW 300
           VWFLHITFF+NSACHIWGYR WNT DLSKNNW+FGLLAFGEGWHNNHHAFEYSARHGLQW
Sbjct: 241 VWFLHITFFINSACHIWGYRRWNTTDLSKNNWVFGLLAFGEGWHNNHHAFEYSARHGLQW 300

Query: 301 WEFDLTWWVVLFLQAVGLATDVKLPSKLHKQKLALDHEKV 341
           WEFDLTWWVVLFLQ +GLATDVKLPS+  KQKLA+DH+++
Sbjct: 301 WEFDLTWWVVLFLQTIGLATDVKLPSQGQKQKLAMDHKRI 339

[Close]

BLAST of Lag0031293 vs. ExPASy TrEMBL
Match: A0A6J1DIZ7 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS=Momordica charantia OX=3673 GN=LOC111020970 PE=3 SV=1)

HSP 1 Score: 610.9 bits (1574), Expect = 3.2e-171
Identity = 285/335 (85.07%), Postives = 310/335 (92.54%), Query Frame = 0


Query: 1   MEATRKQLEDHKDPKKVAFSNGIVMMKPKQAFSKRRWTILDKRSAGGLLFWHLLCLLAPF 60
           MEATRK++ED KD KKVAFS    ++KP++   KR+WTILD+RSAG +LF HLL LLAPF
Sbjct: 1   MEATRKEVEDQKD-KKVAFS----VVKPRRPLLKRKWTILDRRSAGAILFLHLLSLLAPF 60

Query: 61  HCNWAAFWVAFALYFITGLFGITLSYHRNLAHRSFKLPKWLEYSFAYLGLHAFQGDPIDW 120
           HCNWAAFWVAFALYFITGLFGITLSYHRNLAH+SFKLPKWLEY+FAYLG+HAFQGDPIDW
Sbjct: 61  HCNWAAFWVAFALYFITGLFGITLSYHRNLAHKSFKLPKWLEYAFAYLGVHAFQGDPIDW 120

Query: 121 VSTHRYHHQFVDTERDPHSPLEGFWFSHITWMFDAYTLTKKVCPKNIEGFEEKDAKRKRF 180
           VSTHRYHH+FVDTERDPHSPLEGFWFSHI WMFDAY LT+KVCPKN++ F+E+DAKRKRF
Sbjct: 121 VSTHRYHHRFVDTERDPHSPLEGFWFSHIIWMFDAYALTEKVCPKNVQDFKERDAKRKRF 180

Query: 181 SNIFVAQVKYGRPNNVEDLQKQAFYRFLRRTYILHPTALALVLYAIGGLPFVVWGMGVRL 240
           SNIFV  VKYGRPNNVEDLQKQAFYRFLR+TYILHPTALA+VLYA GGLPF+VWGMGVRL
Sbjct: 181 SNIFVGFVKYGRPNNVEDLQKQAFYRFLRKTYILHPTALAIVLYATGGLPFLVWGMGVRL 240

Query: 241 VWFLHITFFVNSACHIWGYRLWNTADLSKNNWLFGLLAFGEGWHNNHHAFEYSARHGLQW 300
           VW LHITFFVNSACHIWGYR W+T DLSKNNW  GLLAFGEGWHNNHHAFEYSARHGLQW
Sbjct: 241 VWVLHITFFVNSACHIWGYRRWDTNDLSKNNWWIGLLAFGEGWHNNHHAFEYSARHGLQW 300

Query: 301 WEFDLTWWVVLFLQAVGLATDVKLPSKLHKQKLAL 336
           WEFDLTW VVL LQA+GLATDVKLPS++HKQKLAL
Sbjct: 301 WEFDLTWCVVLVLQALGLATDVKLPSQIHKQKLAL 330

[Close]

BLAST of Lag0031293 vs. ExPASy TrEMBL
Match: A0A6J1GCE1 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111452892 PE=3 SV=1)

HSP 1 Score: 578.2 bits (1489), Expect = 2.3e-161
Identity = 265/325 (81.54%), Postives = 290/325 (89.23%), Query Frame = 0


Query: 12  KDPKKVAFSNGIVMMKPKQAFSKRRWTILDKRSAGGLLFWHLLCLLAPFHCNWAAFWVAF 71
           ++ KKVA S    M+K K A   R+WTILD+RSAGG+LF H LCLLAPFHCNW AFWVAF
Sbjct: 2   RNTKKVALS----MVKSKPALLNRKWTILDRRSAGGVLFLHFLCLLAPFHCNWPAFWVAF 61

Query: 72  ALYFITGLFGITLSYHRNLAHRSFKLPKWLEYSFAYLGLHAFQGDPIDWVSTHRYHHQFV 131
           ALYFITGLFG+TLSYHRNLAH+SFKLPKWLEY FAYLG+ AFQGDPIDWVSTHRYHHQFV
Sbjct: 62  ALYFITGLFGVTLSYHRNLAHKSFKLPKWLEYLFAYLGVQAFQGDPIDWVSTHRYHHQFV 121

Query: 132 DTERDPHSPLEGFWFSHITWMFDAYTLTKKVCPKNIEGFEEKDAKRKRFSNIFVAQVKYG 191
           DT+RDPHSPL+GFWFSH+ WMFDAYTLTKKV PKN++ FEE+  KRKRF N+ V  VKYG
Sbjct: 122 DTDRDPHSPLDGFWFSHLAWMFDAYTLTKKVYPKNVQDFEERSTKRKRFRNVLVGYVKYG 181

Query: 192 RPNNVEDLQKQAFYRFLRRTYILHPTALALVLYAIGGLPFVVWGMGVRLVWFLHITFFVN 251
           RPNNVEDLQKQ FYRFLR+TYILHPTA A+VLYA GGLPFVVWGMGVRLVW LHITFFVN
Sbjct: 182 RPNNVEDLQKQGFYRFLRKTYILHPTAPAIVLYATGGLPFVVWGMGVRLVWVLHITFFVN 241

Query: 252 SACHIWGYRLWNTADLSKNNWLFGLLAFGEGWHNNHHAFEYSARHGLQWWEFDLTWWVVL 311
           SACHIWGYRLW+T DLSKNNW+ GLLAFGEGWHNNHHAFEYSARHG +WWEFDLTW V+L
Sbjct: 242 SACHIWGYRLWDTNDLSKNNWVIGLLAFGEGWHNNHHAFEYSARHGYEWWEFDLTWCVIL 301

Query: 312 FLQAVGLATDVKLPSKLHKQKLALD 337
           FLQA+GLATDVKLPS  H+QKLAL+
Sbjct: 302 FLQALGLATDVKLPSARHQQKLALN 322

[Close]

BLAST of Lag0031293 vs. ExPASy TrEMBL
Match: A0A0A0KWV4 (Delta 9 desaturase OS=Cucumis sativus OX=3659 GN=Csa_4G006140 PE=3 SV=1)

HSP 1 Score: 551.6 bits (1420), Expect = 2.3e-153
Identity = 253/338 (74.85%), Postives = 288/338 (85.21%), Query Frame = 0


Query: 1   MEATRKQLEDHKDPKKVAFSNGIVMMKPKQAFSKRRWTILDKRSAGGLLFWHLLCLLAPF 60
           MEA RK++ED  D KK  FS    M+ PK+AF  R+WT LD+R+A G+LF HLL LLAPF
Sbjct: 1   MEAARKEVEDKHDKKK-QFS----MVNPKRAFFNRKWTTLDRRTAAGILFLHLLSLLAPF 60

Query: 61  HCNWAAFWVAFALYFITGLFGITLSYHRNLAHRSFKLPKWLEYSFAYLGLHAFQGDPIDW 120
           H NWAAFWVAFALY ITG  GITLSYHRNLAHRSFKLPKWLEY FAYLG+  FQGDPIDW
Sbjct: 61  HFNWAAFWVAFALYLITGALGITLSYHRNLAHRSFKLPKWLEYFFAYLGVQGFQGDPIDW 120

Query: 121 VSTHRYHHQFVDTERDPHSPLEGFWFSHITWMFDAYTLTKKVCPKNIEGFEEKDAKRKRF 180
           VSTHRYHHQFVDT+RDPHSP++GFWFSH+TWMFD+Y LT+K+ PKN+E F+E++AKR   
Sbjct: 121 VSTHRYHHQFVDTDRDPHSPMQGFWFSHMTWMFDSYGLTEKIYPKNLEDFKEREAKRTLS 180

Query: 181 SNIFVAQVKYGRPNNVEDLQKQAFYRFLRRTYILHPTALALVLYAIGGLPFVVWGMGVRL 240
            N FV  +KYGRP NVEDLQKQ FYRFLR+TY+LHP ALA+VLY  GGLPFVVWGMGVRL
Sbjct: 181 RNWFVGFLKYGRPKNVEDLQKQGFYRFLRKTYLLHPIALAIVLYKFGGLPFVVWGMGVRL 240

Query: 241 VWFLHITFFVNSACHIWGYRLWNTADLSKNNWLFGLLAFGEGWHNNHHAFEYSARHGLQW 300
           +  LH   FVNSACHIWGYR WNT DLSKNNW+ GL++FGEGWHNNHHAFEYSARHGLQW
Sbjct: 241 ICVLHAALFVNSACHIWGYRKWNTNDLSKNNWIIGLISFGEGWHNNHHAFEYSARHGLQW 300

Query: 301 WEFDLTWWVVLFLQAVGLATDVKLPSKLHKQKLALDHE 339
           WEFD TWWV+L LQA+GLATDVKLP++LHKQKLA+ ++
Sbjct: 301 WEFDFTWWVILLLQALGLATDVKLPTQLHKQKLAIQYK 333

[Close]

BLAST of Lag0031293 vs. ExPASy TrEMBL
Match: A0A6J1GVF3 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111457787 PE=3 SV=1)

HSP 1 Score: 544.3 bits (1401), Expect = 3.7e-151
Identity = 252/335 (75.22%), Postives = 282/335 (84.18%), Query Frame = 0


Query: 1   MEATRKQLEDHKDPKKVAFSNGIVMMKPKQAFSKRRWTILDKRSAGGLLFWHLLCLLAPF 60
           MEATRK++ED  D KK  FS    ++ PK+ F  R+WT LD+R+A G+LF H+L L APF
Sbjct: 1   MEATRKEVEDKYDKKK-PFS----VVNPKRPFLNRKWTTLDRRNAAGILFLHVLGLFAPF 60

Query: 61  HCNWAAFWVAFALYFITGLFGITLSYHRNLAHRSFKLPKWLEYSFAYLGLHAFQGDPIDW 120
           H NW AFWVAF LYF TG+ GITLSYHRNLAHRSFKLPKWLEYSFAYLG+ AFQGDPIDW
Sbjct: 61  HYNWPAFWVAFTLYFTTGVLGITLSYHRNLAHRSFKLPKWLEYSFAYLGVQAFQGDPIDW 120

Query: 121 VSTHRYHHQFVDTERDPHSPLEGFWFSHITWMFDAYTLTKKVCPKNIEGFEEKDAKRKRF 180
           VSTHRYHHQFVDTERDPHSPLEGFWFSH+TWMFD+Y LT K+ PK +E FEE+DAKR   
Sbjct: 121 VSTHRYHHQFVDTERDPHSPLEGFWFSHVTWMFDSYGLTNKIYPKYLEDFEERDAKRTLS 180

Query: 181 SNIFVAQVKYGRPNNVEDLQKQAFYRFLRRTYILHPTALALVLYAIGGLPFVVWGMGVRL 240
            N FV  +KYGRP NVEDLQK+ FYRFLR+TY+LHP ALA+VLY  GGLPFVVWGMGVRL
Sbjct: 181 RNWFVGFLKYGRPKNVEDLQKEGFYRFLRKTYLLHPIALAMVLYGFGGLPFVVWGMGVRL 240

Query: 241 VWFLHITFFVNSACHIWGYRLWNTADLSKNNWLFGLLAFGEGWHNNHHAFEYSARHGLQW 300
           +  LH  FFVNSACHIWGYR WNT DLSKNNW+ GL++ GEGWHNNHHAFEYSARHGLQW
Sbjct: 241 ICVLHAAFFVNSACHIWGYRKWNTNDLSKNNWVIGLVSCGEGWHNNHHAFEYSARHGLQW 300

Query: 301 WEFDLTWWVVLFLQAVGLATDVKLPSKLHKQKLAL 336
           WEFD TWWV+L LQA+GLATDVKLPS+ H QKLA+
Sbjct: 301 WEFDFTWWVILLLQALGLATDVKLPSQRHMQKLAI 330

[Close]

BLAST of Lag0031293 vs. TAIR 10
Match: AT3G15850.1 (fatty acid desaturase 5 )

HSP 1 Score: 421.4 bits (1082), Expect = 6.9e-118
Identity = 194/322 (60.25%), Postives = 234/322 (72.67%), Query Frame = 0


Query: 13  DPKKVAFSNGIVMMKPKQAFSKRRWTILDKRSAGGLLFWHLLCLLAPFHCNWAAFWVAFA 72
           D +++  S+ +V  K K  + +R W  +D  +   +L  HLL LLAPF  NW A  VAF 
Sbjct: 74  DYRRIMLSDVLVKKKEKVVWWEREWKAMDFGAVAVVLSMHLLSLLAPFQFNWRAVSVAFG 133

Query: 73  LYFITGLFGITLSYHRNLAHRSFKLPKWLEYSFAYLGLHAFQGDPIDWVSTHRYHHQFVD 132
           LY +TGL GITLS+HRNL+H++FKLPKWLEY FAY G  A QG+PIDWVSTHRYHHQF D
Sbjct: 134 LYIVTGLLGITLSFHRNLSHKAFKLPKWLEYLFAYCGAQALQGNPIDWVSTHRYHHQFCD 193

Query: 133 TERDPHSPLEGFWFSHITWMFDAYTLTKKVCPKNIEGFEEKDAKRKRFSNIFVAQVKYGR 192
           ++RDPHSPL+GFWFSH+ WMFD  T+T++                             G 
Sbjct: 194 SDRDPHSPLDGFWFSHMNWMFDTNTITQRC----------------------------GE 253

Query: 193 PNNVEDLQKQAFYRFLRRTYILHPTALALVLYAIGGLPFVVWGMGVRLVWFLHITFFVNS 252
           PNNV DL+KQ FYRFLR TYILHP ALA+ LYA+GG PF+VWGMGVR+VW  HIT+ VNS
Sbjct: 254 PNNVGDLEKQPFYRFLRTTYILHPLALAVALYAMGGFPFIVWGMGVRIVWVYHITWLVNS 313

Query: 253 ACHIWGYRLWNTADLSKNNWLFGLLAFGEGWHNNHHAFEYSARHGLQWWEFDLTWWVVLF 312
           ACH+WG + WNT DLSKNNW    LAFGEGWHNNHHAFE+SARHGL+WW+ D+TW+VV F
Sbjct: 314 ACHVWGKQAWNTGDLSKNNWWVAALAFGEGWHNNHHAFEFSARHGLEWWQLDMTWYVVKF 367

Query: 313 LQAVGLATDVKLPSKLHKQKLA 335
           LQA+GLATDVKLPS+  KQ++A
Sbjct: 374 LQAIGLATDVKLPSEAQKQRMA 367

[Close]

BLAST of Lag0031293 vs. TAIR 10
Match: AT3G15870.1 (Fatty acid desaturase family protein )

HSP 1 Score: 346.7 bits (888), Expect = 2.2e-95
Identity = 164/326 (50.31%), Postives = 215/326 (65.95%), Query Frame = 0


Query: 10  DHKDPKKVAFSNGIVMMKPKQAFSKRRWTILDKRSAGGLLFWHLLCLLAPFHCNWAAFWV 69
           DH +       + +V+++ K++F +R WT  D       +  HLL LLAPF+ +W AFWV
Sbjct: 64  DHVESSWRRLLSEVVVVRTKRSFWERSWTSWDVSKLVIFVGTHLLSLLAPFYFSWEAFWV 123

Query: 70  AFALYFITGLFGITLSYHRNLAHRSFKLPKWLEYSFAYLGLHAFQGDPIDWVSTHRYHHQ 129
              L FI G+  ITLSYHRNL+HRSF LPKWLEY FAY G+ AFQGDPI+WVS HRYHH+
Sbjct: 124 FPWLVFINGIC-ITLSYHRNLSHRSFDLPKWLEYLFAYGGVLAFQGDPIEWVSNHRYHHK 183

Query: 130 FVDTERDPHSPLEGFWFSHITWMFDAYTLTKKVCPKNIEGFEEKDAKRKRFSNIFVAQVK 189
             +T+RDPHSP +GFWFSH+ W+FD  ++ +                             
Sbjct: 184 HCETQRDPHSPTQGFWFSHMAWIFDTSSILENC--------------------------- 243

Query: 190 YGRPNNVEDLQKQAFYRFLRRTYILHPTALALVLYAIGGLPFVVWGMGVRLVWFLHITFF 249
            G   NV+DL +Q FYRFL+RT +LH  A + + Y  GG+P +VWG+G+ +   LH+TF 
Sbjct: 244 -GGEENVDDLVRQPFYRFLQRTVLLHMMAYSFLFYFCGGMPLLVWGIGITIAVRLHLTFL 303

Query: 250 VNSACHIWGYRLWNTADLSKNNWLFGLLAFGEGWHNNHHAFEYSARHGLQWWEFDLTWWV 309
           VNS CHIWG R WNT+D SKNNW   +L  GEGWHNNHHAFE+SARHGL+WW+ D+TW +
Sbjct: 304 VNSVCHIWGTRAWNTSDFSKNNWWVAILTLGEGWHNNHHAFEFSARHGLEWWQLDITWCL 360

Query: 310 VLFLQAVGLATDVKLPSKLHKQKLAL 336
           + FL+A+GLAT+VKLP++   +  AL
Sbjct: 364 IRFLEAIGLATNVKLPTETQMKGKAL 360

[Close]

BLAST of Lag0031293 vs. TAIR 10
Match: AT1G06080.1 (delta 9 desaturase 1 )

HSP 1 Score: 345.5 bits (885), Expect = 4.8e-95
Identity = 166/327 (50.76%), Postives = 215/327 (65.75%), Query Frame = 0


Query: 9   EDHKDPKKVAFSNGIVMMKPKQAFSKRRWTILDKRSAGGLLFWHLLCLLAPFHCNWAAFW 68
           E  ++ KK+A ++   M + K+A  +R+W  LD   A   LF H LCLLAPF+  W A  
Sbjct: 7   EKEENNKKMA-ADKAEMGRKKRAMWERKWKRLDIVKAFASLFVHFLCLLAPFNFTWPALR 66

Query: 69  VAFALYFITGLFGITLSYHRNLAHRSFKLPKWLEYSFAYLGLHAFQGDPIDWVSTHRYHH 128
           VA  +Y + GL GIT+SYHRNLAHRSFK+PKWLEY FAY GL A QGDPIDWVSTHRYHH
Sbjct: 67  VALIVYTVGGL-GITVSYHRNLAHRSFKVPKWLEYFFAYCGLLAIQGDPIDWVSTHRYHH 126

Query: 129 QFVDTERDPHSPLEGFWFSHITWMFDAYTLTKKVCPKNIEGFEEKDAKRKRFSNIFVAQV 188
           QF D++RDPHSP EGFWFSH+ W+FD   L +K                           
Sbjct: 127 QFTDSDRDPHSPNEGFWFSHLLWLFDTGYLVEKC-------------------------- 186

Query: 189 KYGRPNNVEDLQKQAFYRFLRRTYILHPTALALVLYAIGGLPFVVWGMGVRLVWFLHITF 248
             GR  NVEDL++Q +Y+FL+RT + H      +LY  GGL F+ WGMG+ +    H+T 
Sbjct: 187 --GRRTNVEDLKRQWYYKFLQRTVLYHILTFGFLLYYFGGLSFLTWGMGIGVAMEHHVTC 246

Query: 249 FVNSACHIWGYRLWNTADLSKNNWLFGLLAFGEGWHNNHHAFEYSARHGLQWWEFDLTWW 308
            +NS CH+WG R W T D S+N W   + +FGE WHNNHHAFE SAR GL+WW+ D++W+
Sbjct: 247 LINSLCHVWGSRTWKTNDTSRNVWWLSVFSFGESWHNNHHAFESSARQGLEWWQIDISWY 303

Query: 309 VVLFLQAVGLATDVKLPSKLHKQKLAL 336
           +V FL+ +GLATDVKLPS+  ++++A+
Sbjct: 307 IVRFLEIIGLATDVKLPSESQRRRMAM 303

[Close]

BLAST of Lag0031293 vs. TAIR 10
Match: AT2G31360.1 (16:0delta9 desaturase 2 )

HSP 1 Score: 335.1 bits (858), Expect = 6.5e-92
Identity = 158/309 (51.13%), Postives = 198/309 (64.08%), Query Frame = 0


Query: 27  KPKQAFSKRRWTILDKRSAGGLLFWHLLCLLAPFHCNWAAFWVAFALYFITGLFGITLSY 86
           K +  F  RRW  LD          H L LLAPF+  W+A WV F  Y I GL GIT+SY
Sbjct: 26  KRRWVFWDRRWRRLDYVKFSASFTVHSLALLAPFYFTWSALWVTFLFYTIGGL-GITVSY 85

Query: 87  HRNLAHRSFKLPKWLEYSFAYLGLHAFQGDPIDWVSTHRYHHQFVDTERDPHSPLEGFWF 146
           HRNLAHRSFK+PKWLEY  AY  L A QGDPIDWVSTHRYHHQF D+ERDPHSP EGFWF
Sbjct: 86  HRNLAHRSFKVPKWLEYLLAYCALLAIQGDPIDWVSTHRYHHQFTDSERDPHSPKEGFWF 145

Query: 147 SHITWMFDAYTLTKKVCPKNIEGFEEKDAKRKRFSNIFVAQVKYGRPNNVEDLQKQAFYR 206
           SH+ W++D+  L  K                             GR  NVEDL++Q FYR
Sbjct: 146 SHLLWIYDSAYLVSKC----------------------------GRRANVEDLKRQWFYR 205

Query: 207 FLRRTYILHPTALALVLYAIGGLPFVVWGMGVRLVWFLHITFFVNSACHIWGYRLWNTAD 266
           FL++T + H   L   L+ +GG+ FV WGMGV     +H+T  +NS CHIWG R W T D
Sbjct: 206 FLQKTVLFHILGLGFFLFYLGGMSFVTWGMGVGAALEVHVTCLINSLCHIWGTRTWKTND 265

Query: 267 LSKNNWLFGLLAFGEGWHNNHHAFEYSARHGLQWWEFDLTWWVVLFLQAVGLATDVKLPS 326
            S+N W   + +FGE WHNNHHAFE SAR GL+WW+ D++W++V F + +GLATDVK+P+
Sbjct: 266 TSRNVWWLSVFSFGESWHNNHHAFESSARQGLEWWQIDISWYIVRFFEIIGLATDVKVPT 305

Query: 327 KLHKQKLAL 336
           +  ++++A+
Sbjct: 326 EAQRRRMAI 305

[Close]

BLAST of Lag0031293 vs. TAIR 10
Match: AT1G06120.1 (Fatty acid desaturase family protein )

HSP 1 Score: 323.2 bits (827), Expect = 2.6e-88
Identity = 159/316 (50.32%), Postives = 199/316 (62.97%), Query Frame = 0


Query: 20  SNGIVMMKPKQAFSKRRWTILDKRSAGGLLFWHLLCLLAPFHCNWAAFWVAFALYFITGL 79
           S    + K K+AF  R+WT +D      +   HLLCLLAPF+  W AF  A A+  I+  
Sbjct: 11  SQSKAVRKEKRAFLFRKWTRVDVMRVSAVGAVHLLCLLAPFNYTWEAFRFA-AMVGISTN 70

Query: 80  FGITLSYHRNLAHRSFKLPKWLEYSFAYLGLHAFQGDPIDWVSTHRYHHQFVDTERDPHS 139
             IT SYHRNL HRSFKLPKWLEY FAY  L A QG PIDWVSTHR+HHQF D++RDPHS
Sbjct: 71  LSITFSYHRNLTHRSFKLPKWLEYPFAYSALFALQGHPIDWVSTHRFHHQFTDSDRDPHS 130

Query: 140 PLEGFWFSHITWMFDAYTLTKKVCPKNIEGFEEKDAKRKRFSNIFVAQVKYGRPNNVEDL 199
           P+EGFWFSH+ W+FD   + +K                             G  +NV DL
Sbjct: 131 PIEGFWFSHVFWIFDTSYIREKC----------------------------GGRDNVMDL 190

Query: 200 QKQAFYRFLRRTYILHPTALALVLYAIGGLPFVVWGMGVRLVWFLHITFFVNSACHIWGY 259
           ++Q FYRFL+ T  LH     +++Y  GGLP++ W +GV      H T+ +NSACHIWG 
Sbjct: 191 KQQWFYRFLQNTIGLHILTFWILVYLWGGLPYLTWSVGVGGAIGYHATWLINSACHIWGS 250

Query: 260 RLWNTADLSKNNWLFGLLAFGEGWHNNHHAFEYSARHGLQWWEFDLTWWVVLFLQAVGLA 319
           R WNT D S+N W  G    GE WHNNHHAFE SARHGL+W++ DLTW+++ F Q +GLA
Sbjct: 251 RAWNTKDTSRNIWWLGPFTMGESWHNNHHAFEASARHGLEWYQVDLTWYLIWFFQVLGLA 297

Query: 320 TDVKLPSKLHKQKLAL 336
           TDVKLP+   K+K++L
Sbjct: 311 TDVKLPTDAQKRKMSL 297

The following BLAST results are available for this feature:

BLAST of Lag0031293 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022153442.1	1.9e-186	89.41	palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [M...	[more]
XP_022153467.1	6.6e-171	85.07	palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [M...	[more]
KAG6606803.1	1.5e-162	81.85	Palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic, partia...	[more]
XP_023525371.1	2.8e-161	81.23	palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [C...	[more]
XP_022949581.1	4.7e-161	81.54	palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [C...	[more]

BLAST of Lag0031293 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q949X0	9.7e-117	60.25	Palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic OS=Arab...	[more]
P0DOW3	1.9e-96	50.45	Acyl-CoA 5-desaturase AL21 OS=Anemone leveillei OX=212809 GN=AL21 PE=1 SV=1	[more]
P0DOW2	5.6e-96	52.23	Acyl-CoA C20 Delta5-desaturase OS=Anemone leveillei OX=212809 GN=AL10 PE=1 SV=1	[more]
Q9FV68	4.7e-95	52.63	Icosanoyl-CoA 5-desaturase (Fragment) OS=Limnanthes douglasii OX=28973 PE=1 SV=1	[more]
Q9LVZ3	3.0e-94	50.31	Probable lipid desaturase ADS3.2, chloroplastic OS=Arabidopsis thaliana OX=3702 ...	[more]

BLAST of Lag0031293 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DIX0	9.3e-187	89.41	palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS...	[more]
A0A6J1DIZ7	3.2e-171	85.07	palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS...	[more]
A0A6J1GCE1	2.3e-161	81.54	palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS...	[more]
A0A0A0KWV4	2.3e-153	74.85	Delta 9 desaturase OS=Cucumis sativus OX=3659 GN=Csa_4G006140 PE=3 SV=1	[more]
A0A6J1GVF3	3.7e-151	75.22	palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS...	[more]

BLAST of Lag0031293 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G15850.1	6.9e-118	60.25	fatty acid desaturase 5	[more]
AT3G15870.1	2.2e-95	50.31	Fatty acid desaturase family protein	[more]
AT1G06080.1	4.8e-95	50.76	delta 9 desaturase 1	[more]
AT2G31360.1	6.5e-92	51.13	16:0delta9 desaturase 2	[more]
AT1G06120.1	2.6e-88	50.32	Fatty acid desaturase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR015876	Acyl-CoA desaturase	PRINTS	PR00075	FACDDSATRASE	coord: 64..86 score: 23.11 coord: 87..107 score: 33.33 coord: 124..153 score: 49.3
IPR015876	Acyl-CoA desaturase	PANTHER	PTHR11351	ACYL-COA DESATURASE	coord: 12..176
IPR015876	Acyl-CoA desaturase	PANTHER	PTHR11351	ACYL-COA DESATURASE	coord: 189..337
IPR015876	Acyl-CoA desaturase	CDD	cd03505	Delta9-FADS-like	coord: 64..323 e-value: 4.83685E-74 score: 224.742
IPR005804	Fatty acid desaturase domain	PFAM	PF00487	FA_desaturase	coord: 64..309 e-value: 3.0E-18 score: 66.6
None	No IPR available	PANTHER	PTHR11351:SF94	BNAC05G37460D PROTEIN	coord: 12..176 coord: 189..337

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0031293.1	Lag0031293.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006633	fatty acid biosynthetic process
biological_process	GO:0006629	lipid metabolic process
cellular_component	GO:0005789	endoplasmic reticulum membrane
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0009979	16:0 monogalactosyldiacylglycerol desaturase activity
molecular_function	GO:0016717	oxidoreductase activity, acting on paired donors, with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water