Lag0031270 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0031270
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	Alpha-galactosidase
Location	chr11: 6497990 .. 6499287 (-)
RNA-Seq Expression	Lag0031270
Synteny	Lag0031270

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAGTGTAAACGTTAATTCAGGAGCTGGAAAGGCCTTCTTAAGGTCCCTTTTTCAACAATATGCTGATTGGGGTGTTGATTTTGTGAAACATGATTGTATCTTCGGCGATGATTTGGATTTACCTGAAATAACCTTTGTCTCAGATGTTCTTAAACAACTTAACCGCCCAATATTGTATTCTCTATCTCCTGGTACAAGTGTGACACCAGCCATGGCCAAGGCTGTAAGTGGGCTGGCTAACATGTATAGAATAACAGGTGATGATTGGGATACTTGGAACGACATCGTTTCCCACTTCGATGTTACCCGGGATTTCTCTACTGCTAATATGATAGGTACCACAGACTTGCTAGGTAAATCATGGCCTGATTTAGATATGCTCCCACTGGGGTGGCTTACTGATCCAGGTTCAAACAAAGGCCCACATAGAAAAACTAACCTTAATATAGATGAACAAAGAACGTAGATGACTTTATGGTCTATGTCCAAGTCTCCAATTATGTTTGGAGGAGATTTGAGGAATATTGACGATACGACTTATAGTATCATCACGAATCCTACCTTACTGGAGATTAACTCCTGTAGCTCAAATAATATGGAGTTTCTTAAAATTGCTACAACAAAAGGTTTCAAACAGTAGAGAGCAGATTGTGAAATGGCATTCCAGAGATTTGGAGGCATCAGTTTCACCTATATTGGGTCTCACCAAATGCGCAGATTCAAACGCAGCTGGTTGGATTACTGAAAGTCTTGATCGAGGTCTCGAAAAAATATGTTGGAAGGCAAATCCAGAACACAAATATCAAACACCATTATGTTTATATAAAAGAGGATCTCGAGTTGCAATAGATAAAGAGGCAACTACTCGTCATGATCAAGTGGAACTTCTATCGTTGCCAACTAGCACGGTGGACGTTTGTCTGGATGCTACTTTGAAAAGAAAGCGTAGTTATGAAGAGTTCAATAAGGGGTCATTGGACGCTTGCAAACCATTATTCCGGTCATTGTGCAATTGTGAAGTATAACCAAGCTAAAGCTACTCAGACTGGAGTTCGCTCTTGGGTCGCAACAGGAAGAGGAGGTGAGATCTATCTTGCTTTCTTCAATCTGAACAATGCAAAGACGGTAGTATCAGCGAAGATTTCGGACCTTGGCGCGGTACTTCCAGGCAAGAAATTGACTTCCTGTAAATGTAGAGAGGAATGGTCTAGAAAAGACTATGGACTGATCTCAGGGTCAATAGCAGCACCAGTTGAAAGCCATGGTTCTGCACTGTTTATTATCAATTGTTGCTAA

mRNA sequence

ATGAGTGTAAACGTTAATTCAGGAGCTGGAAAGGCCTTCTTAAGGTCCCTTTTTCAACAATATGCTGATTGGGGTGTTGATTTTGTGAAACATGATTGTATCTTCGGCGATGATTTGGATTTACCTGAAATAACCTTTGTCTCAGATGTTCTTAAACAACTTAACCGCCCAATATTGTATTCTCTATCTCCTGGTACAAGTGTGACACCAGCCATGGCCAAGGCTGTAAGTGGGCTGGCTAACATGTATAGAATAACAGGTGATGATTGGGATACTTGGAACGACATCGTTTCCCACTTCGATGTTACCCGGGATTTCTCTACTGCTAATATGATAGGTACCACAGACTTGCTAGGTAAATCATGGCCTGATTTAGATATGCTCCCACTGGGGTGGCTTACTGATCCAGTATCATCACGAATCCTACCTTACTGGAGATTAACTCCTGTAGCTCAAATAATATGGAGTTTCTTAAAATTGCTACAACAAAAGGTTTCAAACAGTAGAGAGCAGATTGTGAAATGGCATTCCAGAGATTTGGAGGCATCAGTTTCACCTATATTGGGTCTCACCAAATGCGCAGATTCAAACGCAGCTGGTTGGATTACTGAAAGTCTTGATCGAGAAAGCGTAGTTATGAAGAGTTCAATAAGGGGTCATTGGACGCTTGCAAACCATTATTCCGGTCATTGTGCAATTGTGAAGTATAACCAAGCTAAAGCTACTCAGACTGGAGTTCGCTCTTGGGTCGCAACAGGAAGAGGAGGTGAGATCTATCTTGCTTTCTTCAATCTGAACAATGCAAAGACGGTAGTATCAGCGAAGATTTCGGACCTTGGCGCGGTACTTCCAGGCAAGAAATTGACTTCCTGTAAATGTAGAGAGGAATGGTCTAGAAAAGACTATGGACTGATCTCAGGGTCAATAGCAGCACCAGTTGAAAGCCATGGTTCTGCACTGTTTATTATCAATTGTTGCTAA

Coding sequence (CDS)

ATGAGTGTAAACGTTAATTCAGGAGCTGGAAAGGCCTTCTTAAGGTCCCTTTTTCAACAATATGCTGATTGGGGTGTTGATTTTGTGAAACATGATTGTATCTTCGGCGATGATTTGGATTTACCTGAAATAACCTTTGTCTCAGATGTTCTTAAACAACTTAACCGCCCAATATTGTATTCTCTATCTCCTGGTACAAGTGTGACACCAGCCATGGCCAAGGCTGTAAGTGGGCTGGCTAACATGTATAGAATAACAGGTGATGATTGGGATACTTGGAACGACATCGTTTCCCACTTCGATGTTACCCGGGATTTCTCTACTGCTAATATGATAGGTACCACAGACTTGCTAGGTAAATCATGGCCTGATTTAGATATGCTCCCACTGGGGTGGCTTACTGATCCAGTATCATCACGAATCCTACCTTACTGGAGATTAACTCCTGTAGCTCAAATAATATGGAGTTTCTTAAAATTGCTACAACAAAAGGTTTCAAACAGTAGAGAGCAGATTGTGAAATGGCATTCCAGAGATTTGGAGGCATCAGTTTCACCTATATTGGGTCTCACCAAATGCGCAGATTCAAACGCAGCTGGTTGGATTACTGAAAGTCTTGATCGAGAAAGCGTAGTTATGAAGAGTTCAATAAGGGGTCATTGGACGCTTGCAAACCATTATTCCGGTCATTGTGCAATTGTGAAGTATAACCAAGCTAAAGCTACTCAGACTGGAGTTCGCTCTTGGGTCGCAACAGGAAGAGGAGGTGAGATCTATCTTGCTTTCTTCAATCTGAACAATGCAAAGACGGTAGTATCAGCGAAGATTTCGGACCTTGGCGCGGTACTTCCAGGCAAGAAATTGACTTCCTGTAAATGTAGAGAGGAATGGTCTAGAAAAGACTATGGACTGATCTCAGGGTCAATAGCAGCACCAGTTGAAAGCCATGGTTCTGCACTGTTTATTATCAATTGTTGCTAA

Protein sequence

MSVNVNSGAGKAFLRSLFQQYADWGVDFVKHDCIFGDDLDLPEITFVSDVLKQLNRPILYSLSPGTSVTPAMAKAVSGLANMYRITGDDWDTWNDIVSHFDVTRDFSTANMIGTTDLLGKSWPDLDMLPLGWLTDPVSSRILPYWRLTPVAQIIWSFLKLLQQKVSNSREQIVKWHSRDLEASVSPILGLTKCADSNAAGWITESLDRESVVMKSSIRGHWTLANHYSGHCAIVKYNQAKATQTGVRSWVATGRGGEIYLAFFNLNNAKTVVSAKISDLGAVLPGKKLTSCKCREEWSRKDYGLISGSIAAPVESHGSALFIINCC

Homology

[Close]

BLAST of Lag0031270 vs. NCBI nr
Match: XP_038886461.1 (alpha-galactosidase mel1 [Benincasa hispida])

HSP 1 Score: 468.8 bits (1205), Expect = 3.9e-128
Identity = 262/449 (58.35%), Postives = 282/449 (62.81%), Query Frame = 0


Query: 1   MSVNVNSGAGKAFLRSLFQQYADWGVDFVKHDCIFGDDLDLPEITFVSDVLKQLNRPILY 60
           MSVNV SGAGKAFLRSL+QQYADWGVDFVKHDCIFGDDLDL EITFVSDVLKQLN  ILY
Sbjct: 190 MSVNVKSGAGKAFLRSLYQQYADWGVDFVKHDCIFGDDLDLDEITFVSDVLKQLNSTILY 249

Query: 61  SLSPGTSVTPAMAKAVSGLANMYRITGDDWDTWNDIVSHFDVTRDFSTANMIGTTDLLGK 120
           SLSPGTSVTPAMAKAVSGLANMYRITGDDWD+WNDIVSHFDVTRDFSTANMIGT  LLGK
Sbjct: 250 SLSPGTSVTPAMAKAVSGLANMYRITGDDWDSWNDIVSHFDVTRDFSTANMIGTAGLLGK 309

Query: 121 SWPDLDMLPLGWLTDPVSSR---------------ILPYWRLTP---------------V 180
           SWPDLDMLPLGWLTDP S+                 +  W ++                 
Sbjct: 310 SWPDLDMLPLGWLTDPGSNNGPHRTTNLNINEQITQMTLWSMSKSPIMYGGDLRNMDNIT 369

Query: 181 AQII---------------WSFLKLLQQKVSNSREQIVKWHSRDLEASVSPILGLTKCAD 240
             II                 FLK+   KVSN REQI KWHSR LEAS SPILGLTKCA 
Sbjct: 370 LSIITNPTILEINSFSSNNMEFLKISTSKVSNRREQIEKWHSRHLEASASPILGLTKCAY 429

Query: 241 SNAAGWITESLDR--ESVVMKSS------------------------------------- 300
           S+ AGWI+ESL+R  E +  K++                                     
Sbjct: 430 SDTAGWISESLNRGVEKICWKANQEHEYRTPFCLYKRGSRVAIDKEATTRHDQVELLSFP 489

Query: 301 --------------------------------------IRGHWTLANHYSGHCAIVKYNQ 326
                                                 +  + TLANHYSGHCAIVKYNQ
Sbjct: 490 TSTVDVCLDATPKRKRSYEELTRGSFFPCRRHENQKWDLYANGTLANHYSGHCAIVKYNQ 549

[Close]

BLAST of Lag0031270 vs. NCBI nr
Match: XP_022951774.1 (uncharacterized protein LOC111454511 [Cucurbita moschata] >XP_022951775.1 uncharacterized protein LOC111454511 [Cucurbita moschata])

HSP 1 Score: 460.3 bits (1183), Expect = 1.4e-125
Identity = 258/450 (57.33%), Postives = 287/450 (63.78%), Query Frame = 0


Query: 1   MSVNVNSGAGKAFLRSLFQQYADWGVDFVKHDCIFGDDLDLPEITFVSDVLKQLNRPILY 60
           MSVNVNSGAGKAFLRSL+QQ+ADWGVDFVKHDC+FGDDLDLPEI+FVSDVLKQLNRPILY
Sbjct: 189 MSVNVNSGAGKAFLRSLYQQFADWGVDFVKHDCVFGDDLDLPEISFVSDVLKQLNRPILY 248

Query: 61  SLSPGTSVTPAMAKAVSGLANMYRITGDDWDTWNDIVSHFDVTRDFSTANMIGTTDLLGK 120
           SLSPGTSVTPAMAKAVSGL NMYRITGDDWDTWNDIVSHFDVTRDFSTANMIGTT LLGK
Sbjct: 249 SLSPGTSVTPAMAKAVSGLVNMYRITGDDWDTWNDIVSHFDVTRDFSTANMIGTTGLLGK 308

Query: 121 SWPDLDMLPLGWLTDPVS--------------------------SRILPYWRLTPVAQII 180
           SWPDLDMLPLGWLTD  S                          S I+    L  +  + 
Sbjct: 309 SWPDLDMLPLGWLTDQGSNDGPHRRSNLNINEQRTQMTLWCMSKSPIMYGGDLRNIDDMT 368

Query: 181 WS-------------------FLKL-LQQKVSNSREQIVKWHSRDLEASVSPILGLTKCA 240
           +S                   FLK+    KVS  REQI+KWH R L+ SVSPILGLTKCA
Sbjct: 369 YSIITNPTLLEINSFSSNNMEFLKIAATTKVSKCREQIMKWHFRYLKPSVSPILGLTKCA 428

Query: 241 DSNAAGWITESLDR--ESVVMKSS------------------------------------ 300
           DSNA GWITE LDR  E +  K++                                    
Sbjct: 429 DSNAVGWITERLDRGVEKICWKANPELEYQTPLCLYKRGSRVAIDKEATTRHDQVELLSS 488

Query: 301 ----------------------IRGHW-----------------TLANHYSGHCAIVKYN 326
                                 +RG +                 TL NH+SGHCAIVK N
Sbjct: 489 HTSTVDVCLDATSKRKHSSEEFMRGSFFPCSRHENQKWDLYSNGTLGNHHSGHCAIVKTN 548

[Close]

BLAST of Lag0031270 vs. NCBI nr
Match: XP_023002641.1 (uncharacterized protein LOC111496438 [Cucurbita maxima] >XP_023002642.1 uncharacterized protein LOC111496438 [Cucurbita maxima])

HSP 1 Score: 452.6 bits (1163), Expect = 2.9e-123
Identity = 253/450 (56.22%), Postives = 286/450 (63.56%), Query Frame = 0


Query: 1   MSVNVNSGAGKAFLRSLFQQYADWGVDFVKHDCIFGDDLDLPEITFVSDVLKQLNRPILY 60
           MSVNVNSGAGKAFLRSL+QQ+ADWGVDFVKHDC+FGDDLDLPEI+FVSDVLKQLNRPILY
Sbjct: 188 MSVNVNSGAGKAFLRSLYQQFADWGVDFVKHDCVFGDDLDLPEISFVSDVLKQLNRPILY 247

Query: 61  SLSPGTSVTPAMAKAVSGLANMYRITGDDWDTWNDIVSHFDVTRDFSTANMIGTTDLLGK 120
           SLSPGTSVTPAMAKAVSGL NMYRITGDDWDTWNDIVSHFDVTRDFSTANMIGTT LLGK
Sbjct: 248 SLSPGTSVTPAMAKAVSGLVNMYRITGDDWDTWNDIVSHFDVTRDFSTANMIGTTGLLGK 307

Query: 121 SWPDLDMLPLGWLTDPVS--------------------------SRILPYWRLTPVAQII 180
           SWPDLDMLPLGWLTD  S                          S I+    L  +  + 
Sbjct: 308 SWPDLDMLPLGWLTDQGSNDGPHRRSNLNINEQRTQMTLWCMSKSPIMYGGDLRNIDDMT 367

Query: 181 WS-------------------FLKL-LQQKVSNSREQIVKWHSRDLEASVSPILGLTKCA 240
           +S                   FLK+    KVS  REQI+KWH R L+ SVSPIL LTKCA
Sbjct: 368 YSIITNPTLLEINSFSTNNMEFLKIAATTKVSKCREQIMKWHFRYLKPSVSPILSLTKCA 427

Query: 241 DSNAAGWITESLDR--ESVVMKSS------------------------------------ 300
           DSNA GWIT++LDR  E +  K++                                    
Sbjct: 428 DSNAVGWITKTLDRGVEKICWKANPEHEYQTPLCLYKRGSRVAIDKEATTRHDQVELLSS 487

Query: 301 ----------------------IRGHW-----------------TLANHYSGHCAIVKYN 326
                                 +RG +                 TL NH+SGHCAIVK +
Sbjct: 488 RTSAVDVCLDATSKRKRSSEEFMRGSFFPCSRHENQKWDLYSNGTLGNHHSGHCAIVKTS 547

[Close]

BLAST of Lag0031270 vs. NCBI nr
Match: XP_023538510.1 (uncharacterized protein LOC111799267 [Cucurbita pepo subsp. pepo] >XP_023538511.1 uncharacterized protein LOC111799267 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 452.2 bits (1162), Expect = 3.8e-123
Identity = 254/450 (56.44%), Postives = 284/450 (63.11%), Query Frame = 0


Query: 1   MSVNVNSGAGKAFLRSLFQQYADWGVDFVKHDCIFGDDLDLPEITFVSDVLKQLNRPILY 60
           MSVNVNSGAGKAFLRSL+QQ+ADWGVDFVKHDC+FGDDLDLPEI+FVSDVLKQLN PILY
Sbjct: 189 MSVNVNSGAGKAFLRSLYQQFADWGVDFVKHDCVFGDDLDLPEISFVSDVLKQLNCPILY 248

Query: 61  SLSPGTSVTPAMAKAVSGLANMYRITGDDWDTWNDIVSHFDVTRDFSTANMIGTTDLLGK 120
           SLSPGTSVTPAMAK VSGL NMYRITGDDWDTWNDIVSHFDVTRDFSTANMIGTT LLGK
Sbjct: 249 SLSPGTSVTPAMAKGVSGLVNMYRITGDDWDTWNDIVSHFDVTRDFSTANMIGTTGLLGK 308

Query: 121 SWPDLDMLPLGWLTDPVS--------------------------SRILPYWRLTPVAQII 180
           SWPDLDMLPLGWLTD  S                          S I+    L  +  + 
Sbjct: 309 SWPDLDMLPLGWLTDQGSNDGPHRRSNLNINEQRTQMTLWCMSKSPIMYGGDLRNIDDMT 368

Query: 181 WS-------------------FLKL-LQQKVSNSREQIVKWHSRDLEASVSPILGLTKCA 240
           +S                   FLK+    KVS  REQI+KWH R  + SVSPILGLTKCA
Sbjct: 369 YSIITNPTLLEINSFSSNNMEFLKIAATTKVSKCREQIMKWHFRYSKPSVSPILGLTKCA 428

Query: 241 DSNAAGWITESLDR--ESVVMKSS------------------------------------ 300
           DSNA GWITE LDR  E +  K++                                    
Sbjct: 429 DSNAVGWITERLDRGVEKICWKANPELEYQTPLCLYKRGSRVAIDKEATTRLDQVELLSS 488

Query: 301 ----------------------IRGHW-----------------TLANHYSGHCAIVKYN 326
                                 +RG +                 TL NH+SGHCAIVK +
Sbjct: 489 PTSTVDVCLDATSKRKRSSEEFVRGSFFRCSRHENQKWDLYSNGTLGNHHSGHCAIVKTD 548

[Close]

BLAST of Lag0031270 vs. NCBI nr
Match: KAA0061052.1 (Melibiase domain-containing protein [Cucumis melo var. makuwa])

HSP 1 Score: 451.4 bits (1160), Expect = 6.4e-123
Identity = 253/446 (56.73%), Postives = 281/446 (63.00%), Query Frame = 0


Query: 1   MSVNVNSGAGKAFLRSLFQQYADWGVDFVKHDCIFGDDLDLPEITFVSDVLKQLNRPILY 60
           MSVNV SGAGKAFLRSL+QQYADWGVDFVKHDC+FGDDLDL EITFVSDVLKQLN  I+Y
Sbjct: 184 MSVNVKSGAGKAFLRSLYQQYADWGVDFVKHDCVFGDDLDLDEITFVSDVLKQLNSTIVY 243

Query: 61  SLSPGTSVTPAMAKAVSGLANMYRITGDDWDTWNDIVSHFDVTRDFSTANMIGTTDLLGK 120
           SLSPGTS TPAMAKAVSGLANMYRITGDDWDTWNDIVSHFDVTRDF+TANMIGTT LLGK
Sbjct: 244 SLSPGTSATPAMAKAVSGLANMYRITGDDWDTWNDIVSHFDVTRDFATANMIGTTGLLGK 303

Query: 121 SWPDLDMLPLGWLTDPVSSR---------------ILPYWRLT----------------- 180
           SWPDLDMLPLGWLTDP S+                 +  W ++                 
Sbjct: 304 SWPDLDMLPLGWLTDPGSNNGPHRTTNLNIDEQRTQMTLWSISKSPIMFGGDLRNIDNTT 363

Query: 181 ------PVAQIIWSF----LKLLQQKVSNSREQIVKWHSRDLEASVSPILGLTKCADSNA 240
                 P    I SF    ++ L+   +N R++IVKWHSR LEAS SPILGLTKCA S+ 
Sbjct: 364 FSIITNPTLLEINSFSSNNMEFLKIASTNFRKRIVKWHSRGLEASASPILGLTKCAYSDT 423

Query: 241 AGWITESLDR--ESVVMKSS---------------------------------------- 300
            GWIT+S+D+  E +  K++                                        
Sbjct: 424 TGWITKSVDQGLEKICWKANPEHEYQTPFCLYKRGSRVAIDKEAATHRDQVELLSFSTSS 483

Query: 301 ------------------IRGHW-----------------TLANHYSGHCAIVKYNQAKA 326
                             +RG +                 TLANH SGHCAIVKY QAKA
Sbjct: 484 VEVCLDATPKRKHSSEAIMRGSFFPCKRHENQKWDLYANGTLANHNSGHCAIVKYKQAKA 543

[Close]

BLAST of Lag0031270 vs. ExPASy Swiss-Prot
Match: P14749 (Alpha-galactosidase OS=Cyamopsis tetragonoloba OX=3832 PE=1 SV=1)

HSP 1 Score: 51.2 bits (121), Expect = 2.5e-05
Identity = 65/308 (21.10%), Postives = 118/308 (38.31%), Query Frame = 0


Query: 21  YADWGVDFVKHDCIFGDDLDLPE-ITFVSDVLKQLNRPILYSLSP-GTSVTPAMAKAVSG 80
           +A WGVD++K+D      + + E    +   L    RPI +S+   G       AK++  
Sbjct: 166 FASWGVDYLKYDNCENLGISVKERYPPMGKALLSSGRPIFFSMCEWGWEDPQIWAKSI-- 225

Query: 81  LANMYRITGDDWDTWNDIVSHFDVTRDFSTANMIGTTDLLGKSWPDLDMLPL---GWLTD 140
             N +R TGD  D WN + S  D    +++    G        W D DML +   G  T+
Sbjct: 226 -GNSWRTTGDIEDNWNSMTSIADSNDKWASYAGPG-------GWNDPDMLEVGNGGMTTE 285

Query: 141 PVSSRILPYWRLTPVAQIIWSFLKLLQQKVSNSREQIVKWHSRDLEASVSPILGLTKCAD 200
              S              IW+  K            +V    R ++ +   ++       
Sbjct: 286 EYRSHF-----------SIWALAK---------APLLVGCDIRAMDDTTHELI------- 345

Query: 201 SNAAGWITESLDRESVVMKSSIRGHWTLANHYSGHCAIVKYNQAKATQTGVRSWVATGRG 260
           SNA          E + +     G              V+  + K+T   +  W      
Sbjct: 346 SNA----------EVIAVNQDKLG--------------VQGKKVKST-NDLEVWAGPLSD 405

Query: 261 GEIYLAFFNLNNAKTVVSAKISDLGAVLPGKKLTSCKCREEWSRKDYGLISGSIAAPVES 320
            ++ +  +N ++++  V+A  SD+G     ++ T+   R+ W      L+SG I+A ++S
Sbjct: 406 NKVAVILWNRSSSRATVTASWSDIGL----QQGTTVDARDLWEHSTQSLVSGEISAEIDS 407

Query: 321 HGSALFII 324
           H   ++++
Sbjct: 466 HACKMYVL 407

[Close]

BLAST of Lag0031270 vs. ExPASy TrEMBL
Match: A0A6J1GJU2 (Alpha-galactosidase OS=Cucurbita moschata OX=3662 GN=LOC111454511 PE=3 SV=1)

HSP 1 Score: 460.3 bits (1183), Expect = 6.7e-126
Identity = 258/450 (57.33%), Postives = 287/450 (63.78%), Query Frame = 0


Query: 1   MSVNVNSGAGKAFLRSLFQQYADWGVDFVKHDCIFGDDLDLPEITFVSDVLKQLNRPILY 60
           MSVNVNSGAGKAFLRSL+QQ+ADWGVDFVKHDC+FGDDLDLPEI+FVSDVLKQLNRPILY
Sbjct: 189 MSVNVNSGAGKAFLRSLYQQFADWGVDFVKHDCVFGDDLDLPEISFVSDVLKQLNRPILY 248

Query: 61  SLSPGTSVTPAMAKAVSGLANMYRITGDDWDTWNDIVSHFDVTRDFSTANMIGTTDLLGK 120
           SLSPGTSVTPAMAKAVSGL NMYRITGDDWDTWNDIVSHFDVTRDFSTANMIGTT LLGK
Sbjct: 249 SLSPGTSVTPAMAKAVSGLVNMYRITGDDWDTWNDIVSHFDVTRDFSTANMIGTTGLLGK 308

Query: 121 SWPDLDMLPLGWLTDPVS--------------------------SRILPYWRLTPVAQII 180
           SWPDLDMLPLGWLTD  S                          S I+    L  +  + 
Sbjct: 309 SWPDLDMLPLGWLTDQGSNDGPHRRSNLNINEQRTQMTLWCMSKSPIMYGGDLRNIDDMT 368

Query: 181 WS-------------------FLKL-LQQKVSNSREQIVKWHSRDLEASVSPILGLTKCA 240
           +S                   FLK+    KVS  REQI+KWH R L+ SVSPILGLTKCA
Sbjct: 369 YSIITNPTLLEINSFSSNNMEFLKIAATTKVSKCREQIMKWHFRYLKPSVSPILGLTKCA 428

Query: 241 DSNAAGWITESLDR--ESVVMKSS------------------------------------ 300
           DSNA GWITE LDR  E +  K++                                    
Sbjct: 429 DSNAVGWITERLDRGVEKICWKANPELEYQTPLCLYKRGSRVAIDKEATTRHDQVELLSS 488

Query: 301 ----------------------IRGHW-----------------TLANHYSGHCAIVKYN 326
                                 +RG +                 TL NH+SGHCAIVK N
Sbjct: 489 HTSTVDVCLDATSKRKHSSEEFMRGSFFPCSRHENQKWDLYSNGTLGNHHSGHCAIVKTN 548

[Close]

BLAST of Lag0031270 vs. ExPASy TrEMBL
Match: A0A6J1KK35 (Alpha-galactosidase OS=Cucurbita maxima OX=3661 GN=LOC111496438 PE=3 SV=1)

HSP 1 Score: 452.6 bits (1163), Expect = 1.4e-123
Identity = 253/450 (56.22%), Postives = 286/450 (63.56%), Query Frame = 0


Query: 1   MSVNVNSGAGKAFLRSLFQQYADWGVDFVKHDCIFGDDLDLPEITFVSDVLKQLNRPILY 60
           MSVNVNSGAGKAFLRSL+QQ+ADWGVDFVKHDC+FGDDLDLPEI+FVSDVLKQLNRPILY
Sbjct: 188 MSVNVNSGAGKAFLRSLYQQFADWGVDFVKHDCVFGDDLDLPEISFVSDVLKQLNRPILY 247

Query: 61  SLSPGTSVTPAMAKAVSGLANMYRITGDDWDTWNDIVSHFDVTRDFSTANMIGTTDLLGK 120
           SLSPGTSVTPAMAKAVSGL NMYRITGDDWDTWNDIVSHFDVTRDFSTANMIGTT LLGK
Sbjct: 248 SLSPGTSVTPAMAKAVSGLVNMYRITGDDWDTWNDIVSHFDVTRDFSTANMIGTTGLLGK 307

Query: 121 SWPDLDMLPLGWLTDPVS--------------------------SRILPYWRLTPVAQII 180
           SWPDLDMLPLGWLTD  S                          S I+    L  +  + 
Sbjct: 308 SWPDLDMLPLGWLTDQGSNDGPHRRSNLNINEQRTQMTLWCMSKSPIMYGGDLRNIDDMT 367

Query: 181 WS-------------------FLKL-LQQKVSNSREQIVKWHSRDLEASVSPILGLTKCA 240
           +S                   FLK+    KVS  REQI+KWH R L+ SVSPIL LTKCA
Sbjct: 368 YSIITNPTLLEINSFSTNNMEFLKIAATTKVSKCREQIMKWHFRYLKPSVSPILSLTKCA 427

Query: 241 DSNAAGWITESLDR--ESVVMKSS------------------------------------ 300
           DSNA GWIT++LDR  E +  K++                                    
Sbjct: 428 DSNAVGWITKTLDRGVEKICWKANPEHEYQTPLCLYKRGSRVAIDKEATTRHDQVELLSS 487

Query: 301 ----------------------IRGHW-----------------TLANHYSGHCAIVKYN 326
                                 +RG +                 TL NH+SGHCAIVK +
Sbjct: 488 RTSAVDVCLDATSKRKRSSEEFMRGSFFPCSRHENQKWDLYSNGTLGNHHSGHCAIVKTS 547

[Close]

BLAST of Lag0031270 vs. ExPASy TrEMBL
Match: A0A5A7UYS7 (Alpha-galactosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold501G001900 PE=3 SV=1)

HSP 1 Score: 451.4 bits (1160), Expect = 3.1e-123
Identity = 253/446 (56.73%), Postives = 281/446 (63.00%), Query Frame = 0


Query: 1   MSVNVNSGAGKAFLRSLFQQYADWGVDFVKHDCIFGDDLDLPEITFVSDVLKQLNRPILY 60
           MSVNV SGAGKAFLRSL+QQYADWGVDFVKHDC+FGDDLDL EITFVSDVLKQLN  I+Y
Sbjct: 184 MSVNVKSGAGKAFLRSLYQQYADWGVDFVKHDCVFGDDLDLDEITFVSDVLKQLNSTIVY 243

Query: 61  SLSPGTSVTPAMAKAVSGLANMYRITGDDWDTWNDIVSHFDVTRDFSTANMIGTTDLLGK 120
           SLSPGTS TPAMAKAVSGLANMYRITGDDWDTWNDIVSHFDVTRDF+TANMIGTT LLGK
Sbjct: 244 SLSPGTSATPAMAKAVSGLANMYRITGDDWDTWNDIVSHFDVTRDFATANMIGTTGLLGK 303

Query: 121 SWPDLDMLPLGWLTDPVSSR---------------ILPYWRLT----------------- 180
           SWPDLDMLPLGWLTDP S+                 +  W ++                 
Sbjct: 304 SWPDLDMLPLGWLTDPGSNNGPHRTTNLNIDEQRTQMTLWSISKSPIMFGGDLRNIDNTT 363

Query: 181 ------PVAQIIWSF----LKLLQQKVSNSREQIVKWHSRDLEASVSPILGLTKCADSNA 240
                 P    I SF    ++ L+   +N R++IVKWHSR LEAS SPILGLTKCA S+ 
Sbjct: 364 FSIITNPTLLEINSFSSNNMEFLKIASTNFRKRIVKWHSRGLEASASPILGLTKCAYSDT 423

Query: 241 AGWITESLDR--ESVVMKSS---------------------------------------- 300
            GWIT+S+D+  E +  K++                                        
Sbjct: 424 TGWITKSVDQGLEKICWKANPEHEYQTPFCLYKRGSRVAIDKEAATHRDQVELLSFSTSS 483

Query: 301 ------------------IRGHW-----------------TLANHYSGHCAIVKYNQAKA 326
                             +RG +                 TLANH SGHCAIVKY QAKA
Sbjct: 484 VEVCLDATPKRKHSSEAIMRGSFFPCKRHENQKWDLYANGTLANHNSGHCAIVKYKQAKA 543

[Close]

BLAST of Lag0031270 vs. ExPASy TrEMBL
Match: A0A1S3B9Q1 (Alpha-galactosidase OS=Cucumis melo OX=3656 GN=LOC103487727 PE=3 SV=1)

HSP 1 Score: 449.5 bits (1155), Expect = 1.2e-122
Identity = 252/446 (56.50%), Postives = 280/446 (62.78%), Query Frame = 0


Query: 1   MSVNVNSGAGKAFLRSLFQQYADWGVDFVKHDCIFGDDLDLPEITFVSDVLKQLNRPILY 60
           MSVNV SGAGKAFLRSL+QQYADWGVDFVKHDC+FGDDLDL EITFVSDVLKQLN  I+Y
Sbjct: 184 MSVNVKSGAGKAFLRSLYQQYADWGVDFVKHDCVFGDDLDLDEITFVSDVLKQLNSTIVY 243

Query: 61  SLSPGTSVTPAMAKAVSGLANMYRITGDDWDTWNDIVSHFDVTRDFSTANMIGTTDLLGK 120
           SLSPGTS TPAMAKAVSGLANMYRITGDDWDTWNDIVSHFDVTRDF+TANMIGT  LLGK
Sbjct: 244 SLSPGTSATPAMAKAVSGLANMYRITGDDWDTWNDIVSHFDVTRDFATANMIGTAGLLGK 303

Query: 121 SWPDLDMLPLGWLTDPVSSR---------------ILPYWRLT----------------- 180
           SWPDLDMLPLGWLTDP S+                 +  W ++                 
Sbjct: 304 SWPDLDMLPLGWLTDPGSNNGPHRTTNLNIDEQRTQMTLWSISKSPIMFGGDLRNIDNTT 363

Query: 181 ------PVAQIIWSF----LKLLQQKVSNSREQIVKWHSRDLEASVSPILGLTKCADSNA 240
                 P    I SF    ++ L+   +N R++IVKWHSR LEAS SPILGLTKCA S+ 
Sbjct: 364 FSIITNPTLLEINSFSSNNMEFLKIASTNFRKRIVKWHSRGLEASASPILGLTKCAYSDT 423

Query: 241 AGWITESLDR--ESVVMKSS---------------------------------------- 300
            GWIT+S+D+  E +  K++                                        
Sbjct: 424 TGWITKSVDQGLEKICWKANPEHEYQTPFCLYKRGSRVAIDKEAATHRDQVELLSFSTSS 483

Query: 301 ------------------IRGHW-----------------TLANHYSGHCAIVKYNQAKA 326
                             +RG +                 TLANH SGHCAIVKY QAKA
Sbjct: 484 VEVCLDATPKRKHSSEAIMRGSFFPCKRHENQKWDLYANGTLANHNSGHCAIVKYKQAKA 543

[Close]

BLAST of Lag0031270 vs. ExPASy TrEMBL
Match: A0A0A0LK91 (Alpha-galactosidase OS=Cucumis sativus OX=3659 GN=Csa_2G349640 PE=3 SV=1)

HSP 1 Score: 449.1 bits (1154), Expect = 1.5e-122
Identity = 249/446 (55.83%), Postives = 278/446 (62.33%), Query Frame = 0


Query: 1   MSVNVNSGAGKAFLRSLFQQYADWGVDFVKHDCIFGDDLDLPEITFVSDVLKQLNRPILY 60
           MSVNV SGAGKAFLRSL+QQYADWGVDFVKHDC+FGDDLDL EITFVSDVLKQLN  I+Y
Sbjct: 184 MSVNVKSGAGKAFLRSLYQQYADWGVDFVKHDCVFGDDLDLDEITFVSDVLKQLNSTIVY 243

Query: 61  SLSPGTSVTPAMAKAVSGLANMYRITGDDWDTWNDIVSHFDVTRDFSTANMIGTTDLLGK 120
           SLSPGTS TPAMAKAVSGLANMYRITGDDWD+WNDIVSHFDVTRDF+TANMIGT  LLGK
Sbjct: 244 SLSPGTSATPAMAKAVSGLANMYRITGDDWDSWNDIVSHFDVTRDFATANMIGTAGLLGK 303

Query: 121 SWPDLDMLPLGWLTDPVSSR---------------ILPYWRLT----------------- 180
           SWPDLDMLPLGWLTDP S+                 +  W ++                 
Sbjct: 304 SWPDLDMLPLGWLTDPGSNNGPHRTTNLNINEQRTQMTLWSISKSPIMFGGDLRNIDNTT 363

Query: 181 ------PVAQIIWSF----LKLLQQKVSNSREQIVKWHSRDLEASVSPILGLTKCADSNA 240
                 P    I +F    ++ L+   +N R++IVKWHSR LE S S ILGLTKCA S+ 
Sbjct: 364 FSIITNPTLLEINAFSSNNMEFLKIASTNFRKRIVKWHSRGLETSASRILGLTKCAYSDT 423

Query: 241 AGWITESL--------------------------------DRESVVMKSSI--------- 300
            GWITESL                                D+E+   +  +         
Sbjct: 424 TGWITESLNEGLEKICWKENPEHESQTPFCLYKRGSRVAIDKEAATRRDQVELLSFPTSS 483

Query: 301 --------------------------RGH----W------TLANHYSGHCAIVKYNQAKA 326
                                     +GH    W      TLANHYSGHCAIVKYN+AK+
Sbjct: 484 VDVCLDATPKRKHSSEAIMRGSFFPCKGHENQKWDLYANGTLANHYSGHCAIVKYNKAKS 543

[Close]

BLAST of Lag0031270 vs. TAIR 10
Match: AT3G26380.1 (Melibiase family protein )

HSP 1 Score: 298.9 bits (764), Expect = 5.0e-81
Identity = 175/451 (38.80%), Postives = 225/451 (49.89%), Query Frame = 0


Query: 1   MSVNVNSGAGKAFLRSLFQQYADWGVDFVKHDCIFGDDLDLPEITFVSDVLKQLNRPILY 60
           MSVN   GAGKAFLRSL++QYA+WGVDF+KHDC+FG D ++ EIT+VS+VLK+L+RP+LY
Sbjct: 195 MSVNTKLGAGKAFLRSLYRQYAEWGVDFIKHDCVFGTDFNIEEITYVSEVLKELDRPVLY 254

Query: 61  SLSPGTSVTPAMAKAVSGLANMYRITGDDWDTWNDIVSHFDVTRDFSTANMIGTTDLLGK 120
           S+SPGTSVTP MAK VS L NMYRITGDDWDTW D+ +HFD++RD S ++MIG   L GK
Sbjct: 255 SISPGTSVTPTMAKEVSQLVNMYRITGDDWDTWKDVTAHFDISRDLSASSMIGARGLQGK 314

Query: 121 SWPDLDMLPLGWLTDPVSSRILPYWRLT------PVAQIIWSFLK--------------- 180
           SWPDLDMLPLGWLTD   S + P+               +WS  K               
Sbjct: 315 SWPDLDMLPLGWLTDQ-GSNVGPHRACNLNLEEQKSQMTLWSIAKSPLMFGGDVRNLDAT 374

Query: 181 -------------------------LLQQKVSNSREQIVKWHSRDLEASVSPILGLTKCA 240
                                    +   +VS ++ +    H      S     GLT C 
Sbjct: 375 TYNLITNPTLLEINSYSSNNKEFPYITATRVSRNKHKGYPHHPTGKNISTKHAFGLTSCK 434

Query: 241 DSNAAGWI---------------TESLDR------------------------------- 300
           +  A  W                +E L++                               
Sbjct: 435 EQKANTWFIVDKNRGQICWNQHSSEKLEKPFCLYNRKALLASDKKLKHNQLYQGKLHLHT 494

Query: 301 ----ESVVMKSS--------------------------IRGHWTLANHYSGHCAIVK-YN 326
               +S +  SS                          +  + TL N YSG CA++    
Sbjct: 495 NDKAQSCLAASSQQKLTSKDYSQGALSPCKLDANQMWELHSNGTLENSYSGLCAVLNPVK 554

The following BLAST results are available for this feature:

BLAST of Lag0031270 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038886461.1	3.9e-128	58.35	alpha-galactosidase mel1 [Benincasa hispida]	[more]
XP_022951774.1	1.4e-125	57.33	uncharacterized protein LOC111454511 [Cucurbita moschata] >XP_022951775.1 unchar...	[more]
XP_023002641.1	2.9e-123	56.22	uncharacterized protein LOC111496438 [Cucurbita maxima] >XP_023002642.1 uncharac...	[more]
XP_023538510.1	3.8e-123	56.44	uncharacterized protein LOC111799267 [Cucurbita pepo subsp. pepo] >XP_023538511....	[more]
KAA0061052.1	6.4e-123	56.73	Melibiase domain-containing protein [Cucumis melo var. makuwa]	[more]

BLAST of Lag0031270 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P14749	2.5e-05	21.10	Alpha-galactosidase OS=Cyamopsis tetragonoloba OX=3832 PE=1 SV=1	[more]

BLAST of Lag0031270 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GJU2	6.7e-126	57.33	Alpha-galactosidase OS=Cucurbita moschata OX=3662 GN=LOC111454511 PE=3 SV=1	[more]
A0A6J1KK35	1.4e-123	56.22	Alpha-galactosidase OS=Cucurbita maxima OX=3661 GN=LOC111496438 PE=3 SV=1	[more]
A0A5A7UYS7	3.1e-123	56.73	Alpha-galactosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold501G...	[more]
A0A1S3B9Q1	1.2e-122	56.50	Alpha-galactosidase OS=Cucumis melo OX=3656 GN=LOC103487727 PE=3 SV=1	[more]
A0A0A0LK91	1.5e-122	55.83	Alpha-galactosidase OS=Cucumis sativus OX=3659 GN=Csa_2G349640 PE=3 SV=1	[more]

BLAST of Lag0031270 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G26380.1	5.0e-81	38.80	Melibiase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR013785	Aldolase-type TIM barrel	GENE3D	3.20.20.70	Aldolase class I	coord: 1..152 e-value: 1.2E-32 score: 115.4
IPR002241	Glycoside hydrolase, family 27	PFAM	PF16499	Melibiase_2	coord: 18..131 e-value: 4.0E-5 score: 22.9
IPR002241	Glycoside hydrolase, family 27	PANTHER	PTHR11452	ALPHA-GALACTOSIDASE/ALPHA-N-ACETYLGALACTOSAMINIDASE	coord: 5..140
IPR002241	Glycoside hydrolase, family 27	PANTHER	PTHR11452	ALPHA-GALACTOSIDASE/ALPHA-N-ACETYLGALACTOSAMINIDASE	coord: 216..323
IPR041233	Alpha galactosidase, C-terminal beta sandwich domain	PFAM	PF17801	Melibiase_C	coord: 245..322 e-value: 5.2E-8 score: 32.8
IPR013780	Glycosyl hydrolase, all-beta	GENE3D	2.60.40.1180		coord: 237..325 e-value: 1.3E-20 score: 75.2
None	No IPR available	PANTHER	PTHR11452:SF42	ALPHA-GALACTOSIDASE	coord: 216..323
None	No IPR available	PANTHER	PTHR11452:SF42	ALPHA-GALACTOSIDASE	coord: 5..140
None	No IPR available	SUPERFAMILY	51011	Glycosyl hydrolase domain	coord: 244..323
IPR017853	Glycoside hydrolase superfamily	SUPERFAMILY	51445	(Trans)glycosidases	coord: 10..133

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0031270.1	Lag0031270.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
molecular_function	GO:0004553	hydrolase activity, hydrolyzing O-glycosyl compounds
molecular_function	GO:0003824	catalytic activity