Lag0031267 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0031267
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
Descriptionprotein NUCLEAR FUSION DEFECTIVE 4
Locationchr11: 6421156 .. 6424932 (+)
RNA-Seq ExpressionLag0031267
SyntenyLag0031267
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCCGAAGCTGGTTTTGAAAGGGGGAAGTAGGCCGCCATGGGTGGGCTTGGCTGCTGCTGTTTGGGTTCAAATAGCTGCTGGTTCTTCTTATAACTTTTCTCTTTACTCACACTTGCTTAAATCTGTTCTGGGTTTGAATCAGCAGCAGCTGACTGTGCTGGGAGTGGCTAATGACATTGGGGAGAGTGTTGGTTTGCTTCCTGGACTTGCTTGCAATAGATTTCCTCCATGGGCTGTGCTTTTGGTTGGCTCTCTGTGTTGCTTTGTGGGTTATGGGGCTATTTGGCTTGCTGTTAGCAGGACTGTTCCCAATCTGCCTTATTGGCTGGTGAGTTTCATGTTTATCCTTTCCCTTTCTCAAAATCTCTCTCTCCTTTTGTGGTTATTGTAGCTTTTTATGTGTTCTAGTTGCTGCTGACTTTCAAGAACTTGTTTGGAGGGTGGGGGGTTTTGTTTGTTGTTCATGAATGCAACAGATTGGTTATTTGGTTGCTTTGTTGTTATAAGATAGCCTATGATCTAAGTCCTGGATGTTCCTCTGTTAATGGAGTTGGCTTTTTTTACTGGGTTTTAGGAGATTTTGGATCAAAGCTTCTTAGTAATTAGTCATTACTAGATTGATAAGCTAAGCGCTGTGGATCTTATAAACAACTTATTCTATTAAAAAGGTTAGACAAGGAATCCCCAGAAACCACCTAAATGAGATTATACGAAAAGGAGATCTTCAAGTGCAACGTTTTCCTGTTATTTCCTTCCATCCATGGCTCCCATTAGTTAGCCAAGAAGAATGACTGAGGAATTTTAGATTTTGTTTCGATTGGGTTTGCGGCGAGGGGAGCAAAGAGAAAATCTCTAACACTGAGGTTAAACAAAGTTTGATTATCTTCAAAGTTTAATGGAGTGAAATATGACCAAGAGTTCTCCAAGTATACGAATCAGATCTGGACTCTTTTTTCGAAATAGAGGTAAAATTTTTAACAACATTGACTTTGTTGCATTTTCTTGATTCATTGTCAAAGTTTGCCATTTTAGCACAAAAGAAGAGATGGATGATTGATTGTTGAATAGGTTGAGGGTCAAATTTGAGCAACTTTAGCAGAAATACTTATTTCATTGTTGGGTTTTAAGTCTTAATGGACTTAACAATGTATCTCTACATTAGATTCAGCCATCATTTGTTGAAGAATTTTCATGTATATGATTTCAGTAAGGATGTTGATGATCTGAATGAAACTAAACAAACATGTTGATGTTAATCTGCTCATGCCATCTCATGTAACTCATGGTATCAGTCAGATGAAACTCTATATTCGTTCAATATATAAGGTTGAAGTTGATTACGAGATGAATCCTACTCGAGCATTCAAGCGACTTCAGAACTCTCTCCTACTCGATTCTCAACAAGTTCCCTTCATCCCCTTTCCCATATACTCTTCTACTTCCAACCGATTTTTCATTCTGTGCCATTCTATTATCTACTAATACTACAAAATCCCTTCATTTACATTTTCAAACGAATCAAACCTCCCATGTTTGATTGATGAATATCTGATTAAAGTTTATGATAGAAACTAGAGCATTGAAATAGAAATTGTATCGATAACTTGGAGAATGGAATAAGAATACCTGAGTAAGTAGAGGAACTTGGACACTCCCAAACATTTGTCTGCCTTCACCTTCTCCCATTCACTTTATTTATATTAACCACCCCTTCAAACAGACTCCTTACTCCTTAGTTAACCATTAAAATGGCTTTAGGATTCTAATTTTTCAAGATCTACCCTAAATAGTATTCCTAACAGTTTTACCCCCCCCCCCCTTTTTTAAGTTCAACAACATTTGGGGGTGAGAGGATTTGAGCCGTTGATCTCTTGGTCGAGGGTACATGTCAATTATCGCTGAGATATGCTCACTTTAGCCCTAACAGTTTTACCCTCTCAGCATAGGCAAAGCAGACAGGAGGCCTAACATGTGTAGAAAATGGATGAGATATGAATAATTTCCTTTGTTCATCTATTTGTTCAAAATAATTTCCTTCACTCTTTTCCTAGCCTTTGTAGCATTTTTACCAGCTTTGCTTCTGGATTCTGTTGATTCTAGCTTATGCGCTTTAAACATTTACTTCTGAATGTCATGGCAGCTATGGCTCACACATTGTATTGCTACAAATAGCAATGCATGGTTCGGAACAGCTGTGCTTGTAACCAACATGAGGAACTTCCCTCTCAGCAGAGGTACCGTTGCGGGCATTCTTAAAGGTTATGTCGGGCTTAGTGCTGCTGTATACACCGTGATATACAGCATGGTGCTCAAAAAGTCTGCATTGGATTTACTGTTGTTTCTTGCGATCGGGATACCCATTTTGTGCTTAGCTTTGATGTACTTTGTTCGACCTTGTACTCCGGCTTCTTCAGAAGATCCGTCTGAGCGCAGTCATTTTCTTTTTACGCAAGCTGCTTGTGTCTTGCTAGCTGTTTTTCTAGTCTCGACCACAATTATAGATGCAACAACAACCCCTAGTGATGCCGTTGCATACACCTTAGTTGCCATAATGGCTATCCTTTTAATGTCTCCTCTTGCAGTTCCCATAAAAATGACCATTTGTTCTGCAAAAACGAAAAAACTCGGTCCTCGAGTTGACTCTGCAGAACCTTTGGCTTCCGGAGAAAGCGATTCTTCCCAAATAGAACCTTTATTGACACCATCTTCCTCAGCTACAAATCTTGGAAGTTTCTATGAGAATGATGATGCATCCGATGTAGAAACACTTCTTGCTGTGGGAGAGGGGGCGATTCATAAGAAGAGGAGAAGACCTAAAAGAGGTGAAGATTTCAAGCTCCGTGAAGCCGTAATCAAAGCTGATTTTTGGCTGCTTTGGTTCTTGTACTTCCTCGGGGTCGGTCCGGGAATAACGGTCCTCAACAATTTGTCTCAAATTGGGATTTCATTAGGTGTTAATGACACAACATTGCTGTTGGCTCTTTTCAGCTTCTGCAATTTTATTGGTCGTCTAGGCTCCGGTGTTATTTCCGAGCACTTCGTCAGGTAAATTCTAATACAAAACAGATTACTGAATTAATAATTAGTTCCAGCTTTTGTCGATAAGGTATCTCAGAAACAGCTCCCTTTCGTTCCCAGTTCCAAATCTTCAAATTCAACTATTGTGCAATGTGCAGTACAGTTTTCAGAGTTAACTTAGAGATTTTTGCAAGTTACTTTGGTTTTCTGTTCTGAGTATCATGCGTTTTTCTCATGAAGTTGCTCCATTTACGAAATGCTCATCGGTTCGGCCATCGTTGTTCAATGCAGGTCAAGAATGATACCTCGCTCGCTATGGATGATGTTTGCACTAATACTCATGTCTATAGCATTCCTCCTCTATGCATCCGCCCTCACGAGTACTCTCTACCTCGCCACCGGTTTCACTGGAATTAGCTACGGAATTCTATATTCTATGATGGTCCCTCTCGCATCCGAGATTTTCGGCCTCAAGAATTTCGGTGTAATCTTCAACTTCATGCAGCTGGGGAATCCAATAGGAGCAGTCCTTTTCTCCGTTCTACTCGCCAGTACTTTATACGACAATGAAGCTGCTAAGCAAGGTTCGATAACCTGCATAGGCCAACAGTGCTTCAGGGTAACTTTCTTCGTTCTTTCGGGTGTTGCCGGGTTAGGTTCGATCTTGAGCATAATTCTGACTGTTAGATTGAGGCCAGTCTATCAAATGCTTTACGCTGGTGGCTCGTTCCGCTTGCCTCAAAGTTCAGGTCATTGA

mRNA sequence

ATGCCGAAGCTGGTTTTGAAAGGGGGAAGTAGGCCGCCATGGGTGGGCTTGGCTGCTGCTGTTTGGGTTCAAATAGCTGCTGGTTCTTCTTATAACTTTTCTCTTTACTCACACTTGCTTAAATCTGTTCTGGGTTTGAATCAGCAGCAGCTGACTGTGCTGGGAGTGGCTAATGACATTGGGGAGAGTGTTGGTTTGCTTCCTGGACTTGCTTGCAATAGATTTCCTCCATGGGCTGTGCTTTTGGTTGGCTCTCTGTGTTGCTTTGTGGGTTATGGGGCTATTTGGCTTGCTGTTAGCAGGACTGTTCCCAATCTGCCTTATTGGCTGCTATGGCTCACACATTGTATTGCTACAAATAGCAATGCATGGTTCGGAACAGCTGTGCTTGTAACCAACATGAGGAACTTCCCTCTCAGCAGAGGTACCGTTGCGGGCATTCTTAAAGGTTATGTCGGGCTTAGTGCTGCTGTATACACCGTGATATACAGCATGGTGCTCAAAAAGTCTGCATTGGATTTACTGTTGTTTCTTGCGATCGGGATACCCATTTTGTGCTTAGCTTTGATGTACTTTGTTCGACCTTGTACTCCGGCTTCTTCAGAAGATCCGTCTGAGCGCAGTCATTTTCTTTTTACGCAAGCTGCTTGTGTCTTGCTAGCTGTTTTTCTAGTCTCGACCACAATTATAGATGCAACAACAACCCCTAGTGATGCCGTTGCATACACCTTAGTTGCCATAATGGCTATCCTTTTAATGTCTCCTCTTGCAGTTCCCATAAAAATGACCATTTGTTCTGCAAAAACGAAAAAACTCGGTCCTCGAGTTGACTCTGCAGAACCTTTGGCTTCCGGAGAAAGCGATTCTTCCCAAATAGAACCTTTATTGACACCATCTTCCTCAGCTACAAATCTTGGAAGTTTCTATGAGAATGATGATGCATCCGATGTAGAAACACTTCTTGCTGTGGGAGAGGGGGCGATTCATAAGAAGAGGAGAAGACCTAAAAGAGGTGAAGATTTCAAGCTCCGTGAAGCCGTAATCAAAGCTGATTTTTGGCTGCTTTGGTTCTTGTACTTCCTCGGGGTCGGTCCGGGAATAACGGTCCTCAACAATTTGTCTCAAATTGGGATTTCATTAGGTGTTAATGACACAACATTGCTGTTGGCTCTTTTCAGCTTCTGCAATTTTATTGGTCGTCTAGGCTCCGGTGTTATTTCCGAGCACTTCGTCAGTTGCTCCATTTACGAAATGCTCATCGGTTCGGCCATCGTTGTTCAATGCAGGTCAAGAATGATACCTCGCTCGCTATGGATGATGTTTGCACTAATACTCATGTCTATAGCATTCCTCCTCTATGCATCCGCCCTCACGAGTACTCTCTACCTCGCCACCGGTTTCACTGGAATTAGCTACGGAATTCTATATTCTATGATGGTCCCTCTCGCATCCGAGATTTTCGGCCTCAAGAATTTCGGTGTAATCTTCAACTTCATGCAGCTGGGGAATCCAATAGGAGCAGTCCTTTTCTCCGTTCTACTCGCCAGTACTTTATACGACAATGAAGCTGCTAAGCAAGGTTCGATAACCTGCATAGGCCAACAGTGCTTCAGGGTAACTTTCTTCGTTCTTTCGGGTGTTGCCGGGTTAGGTTCGATCTTGAGCATAATTCTGACTGTTAGATTGAGGCCAGTCTATCAAATGCTTTACGCTGGTGGCTCGTTCCGCTTGCCTCAAAGTTCAGGTCATTGA

Coding sequence (CDS)

ATGCCGAAGCTGGTTTTGAAAGGGGGAAGTAGGCCGCCATGGGTGGGCTTGGCTGCTGCTGTTTGGGTTCAAATAGCTGCTGGTTCTTCTTATAACTTTTCTCTTTACTCACACTTGCTTAAATCTGTTCTGGGTTTGAATCAGCAGCAGCTGACTGTGCTGGGAGTGGCTAATGACATTGGGGAGAGTGTTGGTTTGCTTCCTGGACTTGCTTGCAATAGATTTCCTCCATGGGCTGTGCTTTTGGTTGGCTCTCTGTGTTGCTTTGTGGGTTATGGGGCTATTTGGCTTGCTGTTAGCAGGACTGTTCCCAATCTGCCTTATTGGCTGCTATGGCTCACACATTGTATTGCTACAAATAGCAATGCATGGTTCGGAACAGCTGTGCTTGTAACCAACATGAGGAACTTCCCTCTCAGCAGAGGTACCGTTGCGGGCATTCTTAAAGGTTATGTCGGGCTTAGTGCTGCTGTATACACCGTGATATACAGCATGGTGCTCAAAAAGTCTGCATTGGATTTACTGTTGTTTCTTGCGATCGGGATACCCATTTTGTGCTTAGCTTTGATGTACTTTGTTCGACCTTGTACTCCGGCTTCTTCAGAAGATCCGTCTGAGCGCAGTCATTTTCTTTTTACGCAAGCTGCTTGTGTCTTGCTAGCTGTTTTTCTAGTCTCGACCACAATTATAGATGCAACAACAACCCCTAGTGATGCCGTTGCATACACCTTAGTTGCCATAATGGCTATCCTTTTAATGTCTCCTCTTGCAGTTCCCATAAAAATGACCATTTGTTCTGCAAAAACGAAAAAACTCGGTCCTCGAGTTGACTCTGCAGAACCTTTGGCTTCCGGAGAAAGCGATTCTTCCCAAATAGAACCTTTATTGACACCATCTTCCTCAGCTACAAATCTTGGAAGTTTCTATGAGAATGATGATGCATCCGATGTAGAAACACTTCTTGCTGTGGGAGAGGGGGCGATTCATAAGAAGAGGAGAAGACCTAAAAGAGGTGAAGATTTCAAGCTCCGTGAAGCCGTAATCAAAGCTGATTTTTGGCTGCTTTGGTTCTTGTACTTCCTCGGGGTCGGTCCGGGAATAACGGTCCTCAACAATTTGTCTCAAATTGGGATTTCATTAGGTGTTAATGACACAACATTGCTGTTGGCTCTTTTCAGCTTCTGCAATTTTATTGGTCGTCTAGGCTCCGGTGTTATTTCCGAGCACTTCGTCAGTTGCTCCATTTACGAAATGCTCATCGGTTCGGCCATCGTTGTTCAATGCAGGTCAAGAATGATACCTCGCTCGCTATGGATGATGTTTGCACTAATACTCATGTCTATAGCATTCCTCCTCTATGCATCCGCCCTCACGAGTACTCTCTACCTCGCCACCGGTTTCACTGGAATTAGCTACGGAATTCTATATTCTATGATGGTCCCTCTCGCATCCGAGATTTTCGGCCTCAAGAATTTCGGTGTAATCTTCAACTTCATGCAGCTGGGGAATCCAATAGGAGCAGTCCTTTTCTCCGTTCTACTCGCCAGTACTTTATACGACAATGAAGCTGCTAAGCAAGGTTCGATAACCTGCATAGGCCAACAGTGCTTCAGGGTAACTTTCTTCGTTCTTTCGGGTGTTGCCGGGTTAGGTTCGATCTTGAGCATAATTCTGACTGTTAGATTGAGGCCAGTCTATCAAATGCTTTACGCTGGTGGCTCGTTCCGCTTGCCTCAAAGTTCAGGTCATTGA

Protein sequence

MPKLVLKGGSRPPWVGLAAAVWVQIAAGSSYNFSLYSHLLKSVLGLNQQQLTVLGVANDIGESVGLLPGLACNRFPPWAVLLVGSLCCFVGYGAIWLAVSRTVPNLPYWLLWLTHCIATNSNAWFGTAVLVTNMRNFPLSRGTVAGILKGYVGLSAAVYTVIYSMVLKKSALDLLLFLAIGIPILCLALMYFVRPCTPASSEDPSERSHFLFTQAACVLLAVFLVSTTIIDATTTPSDAVAYTLVAIMAILLMSPLAVPIKMTICSAKTKKLGPRVDSAEPLASGESDSSQIEPLLTPSSSATNLGSFYENDDASDVETLLAVGEGAIHKKRRRPKRGEDFKLREAVIKADFWLLWFLYFLGVGPGITVLNNLSQIGISLGVNDTTLLLALFSFCNFIGRLGSGVISEHFVSCSIYEMLIGSAIVVQCRSRMIPRSLWMMFALILMSIAFLLYASALTSTLYLATGFTGISYGILYSMMVPLASEIFGLKNFGVIFNFMQLGNPIGAVLFSVLLASTLYDNEAAKQGSITCIGQQCFRVTFFVLSGVAGLGSILSIILTVRLRPVYQMLYAGGSFRLPQSSGH
Homology
BLAST of Lag0031267 vs. NCBI nr
Match: KAG7018369.1 (Protein NUCLEAR FUSION DEFECTIVE 4 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1031.9 bits (2667), Expect = 2.1e-297
Identity = 536/583 (91.94%), Postives = 557/583 (95.54%), Query Frame = 0

Query: 1   MPKLVLKGGSRPPWVGLAAAVWVQIAAGSSYNFSLYSHLLKSVLGLNQQQLTVLGVANDI 60
           M KLVLKGGSRPPWVGLAAAVWVQIAAGSSYNFSLYSHLLKSVLGLNQQQLTVLGVANDI
Sbjct: 1   MSKLVLKGGSRPPWVGLAAAVWVQIAAGSSYNFSLYSHLLKSVLGLNQQQLTVLGVANDI 60

Query: 61  GESVGLLPGLACNRFPPWAVLLVGSLCCFVGYGAIWLAVSRTVPNLPYWLLWLTHCIATN 120
           GESVGLLPGLACNRFPPWAVLLVG++CCFVGYGAIWLAVSRTVPNLPYWLLWLTHCIATN
Sbjct: 61  GESVGLLPGLACNRFPPWAVLLVGAVCCFVGYGAIWLAVSRTVPNLPYWLLWLTHCIATN 120

Query: 121 SNAWFGTAVLVTNMRNFPLSRGTVAGILKGYVGLSAAVYTVIYSMVLKKSALDLLLFLAI 180
           SNAWFGTAVLVTNMRNFPLSRGTVAGILKGYVGLSAAVYTVIYSMVL+KSALDL+LFLAI
Sbjct: 121 SNAWFGTAVLVTNMRNFPLSRGTVAGILKGYVGLSAAVYTVIYSMVLRKSALDLVLFLAI 180

Query: 181 GIPILCLALMYFVRPCTPASSEDPSERSHFLFTQAACVLLAVFLVSTTIIDATTTPSDAV 240
           GIPILCLALMYFVRPCTPAS+EDPSER+HFLFTQAACVLLAVFLVSTTI+DATTTPS+AV
Sbjct: 181 GIPILCLALMYFVRPCTPASTEDPSERNHFLFTQAACVLLAVFLVSTTILDATTTPSNAV 240

Query: 241 AYTLVAIMAILLMSPLAVPIKMTICSAKTKKLGPRVDSAEPLASGESDSSQIEPLLTPSS 300
           AYTLVAIM +LLMSPLAVPIKMTICS KTKKLGPRVDS EPLASGESDSSQIEPLLTPSS
Sbjct: 241 AYTLVAIMVVLLMSPLAVPIKMTICSVKTKKLGPRVDSTEPLASGESDSSQIEPLLTPSS 300

Query: 301 SATNLGSFYENDDASDVETLLAVGEGAIHKKRRRPKRGEDFKLREAVIKADFWLLWFLYF 360
           SATNLGSFYEND++SDVETLLAVGEGAIHKK+R+PKRGEDFKLREAVIKADFWLLWFLYF
Sbjct: 301 SATNLGSFYENDESSDVETLLAVGEGAIHKKKRKPKRGEDFKLREAVIKADFWLLWFLYF 360

Query: 361 LGVGPGITVLNNLSQIGISLGVNDTTLLLALFSFCNFIGRLGSGVISEHFVSCSIYEMLI 420
           LGVGPGITVLNNLSQIGISLGVNDTTLLLALFSFCNF+GRLGSGVISEHFV         
Sbjct: 361 LGVGPGITVLNNLSQIGISLGVNDTTLLLALFSFCNFVGRLGSGVISEHFV--------- 420

Query: 421 GSAIVVQCRSRMIPRSLWMMFALILMSIAFLLYASALTSTLYLATGFTGISYGILYSMMV 480
                   RSRMIPRSLWMMFAL+LMS+AFLLYASA T TLY+ATG  GISYG+LY+MMV
Sbjct: 421 --------RSRMIPRSLWMMFALMLMSVAFLLYASAHTVTLYVATGLMGISYGVLYAMMV 480

Query: 481 PLASEIFGLKNFGVIFNFMQLGNPIGAVLFSVLLASTLYDNEAAKQGSITCIGQQCFRVT 540
           PLASEIFGLKNFGVIFNFMQLGNPIGAVLFSVLLASTLYDNEAAKQGSITCIGQ+CFRVT
Sbjct: 481 PLASEIFGLKNFGVIFNFMQLGNPIGAVLFSVLLASTLYDNEAAKQGSITCIGQECFRVT 540

Query: 541 FFVLSGVAGLGSILSIILTVRLRPVYQMLYAGGSFRLPQSSGH 584
           FFVLSGVAGLGSIL IILTVRLRPVYQMLYAGGSFRLPQ+SGH
Sbjct: 541 FFVLSGVAGLGSILCIILTVRLRPVYQMLYAGGSFRLPQNSGH 566

BLAST of Lag0031267 vs. NCBI nr
Match: XP_023528220.1 (protein NUCLEAR FUSION DEFECTIVE 4 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1031.2 bits (2665), Expect = 3.5e-297
Identity = 536/583 (91.94%), Postives = 557/583 (95.54%), Query Frame = 0

Query: 1   MPKLVLKGGSRPPWVGLAAAVWVQIAAGSSYNFSLYSHLLKSVLGLNQQQLTVLGVANDI 60
           M KLVLKGGSRPPWVGLAAAVWVQIAAGSSYNFSLYSHLLKSVLGLNQQQLTVLGVANDI
Sbjct: 1   MSKLVLKGGSRPPWVGLAAAVWVQIAAGSSYNFSLYSHLLKSVLGLNQQQLTVLGVANDI 60

Query: 61  GESVGLLPGLACNRFPPWAVLLVGSLCCFVGYGAIWLAVSRTVPNLPYWLLWLTHCIATN 120
           GESVGLLPGLACNRFPPWAVLLVG++CCFVGYGAIWLAVSRTVPNLPYWLLWLTHCIATN
Sbjct: 61  GESVGLLPGLACNRFPPWAVLLVGAVCCFVGYGAIWLAVSRTVPNLPYWLLWLTHCIATN 120

Query: 121 SNAWFGTAVLVTNMRNFPLSRGTVAGILKGYVGLSAAVYTVIYSMVLKKSALDLLLFLAI 180
           SNAWFGTAVLVTNMRNFPLSRGTVAGILKGYVGLSAAVYTVIYSMVL+KSALDLLLFLAI
Sbjct: 121 SNAWFGTAVLVTNMRNFPLSRGTVAGILKGYVGLSAAVYTVIYSMVLRKSALDLLLFLAI 180

Query: 181 GIPILCLALMYFVRPCTPASSEDPSERSHFLFTQAACVLLAVFLVSTTIIDATTTPSDAV 240
           GIPILCLALMYFVRPCTPAS+EDPSER+HFLFTQAACVLLAVFLVSTTI+DATTTPS+AV
Sbjct: 181 GIPILCLALMYFVRPCTPASTEDPSERNHFLFTQAACVLLAVFLVSTTILDATTTPSNAV 240

Query: 241 AYTLVAIMAILLMSPLAVPIKMTICSAKTKKLGPRVDSAEPLASGESDSSQIEPLLTPSS 300
           AYTLVAIM +LLMSPLAVPIKMTICS KTKKLGPRVDS EPLASGESDSSQIEPLLTPSS
Sbjct: 241 AYTLVAIMVVLLMSPLAVPIKMTICSVKTKKLGPRVDSTEPLASGESDSSQIEPLLTPSS 300

Query: 301 SATNLGSFYENDDASDVETLLAVGEGAIHKKRRRPKRGEDFKLREAVIKADFWLLWFLYF 360
           SATNLGSFYEND++SDVETLLAVGEGAIHKK+R+PKRGEDFKLREAVIKADFWLLWFLYF
Sbjct: 301 SATNLGSFYENDESSDVETLLAVGEGAIHKKKRKPKRGEDFKLREAVIKADFWLLWFLYF 360

Query: 361 LGVGPGITVLNNLSQIGISLGVNDTTLLLALFSFCNFIGRLGSGVISEHFVSCSIYEMLI 420
           LGVGPGITVLNNLSQIGISLGV+DTTLLLALFSFCNF+GRLGSGVISEHFV         
Sbjct: 361 LGVGPGITVLNNLSQIGISLGVDDTTLLLALFSFCNFVGRLGSGVISEHFV--------- 420

Query: 421 GSAIVVQCRSRMIPRSLWMMFALILMSIAFLLYASALTSTLYLATGFTGISYGILYSMMV 480
                   RSRMIPRSLWMMFAL+LMS+AFLLYASA T TLY+ATG  GISYG+LY+MMV
Sbjct: 421 --------RSRMIPRSLWMMFALMLMSVAFLLYASAHTVTLYVATGLMGISYGVLYAMMV 480

Query: 481 PLASEIFGLKNFGVIFNFMQLGNPIGAVLFSVLLASTLYDNEAAKQGSITCIGQQCFRVT 540
           PLASEIFGLKNFGVIFNFMQLGNPIGAVLFSVLLASTLYDNEAAKQGSITCIGQ+CFRVT
Sbjct: 481 PLASEIFGLKNFGVIFNFMQLGNPIGAVLFSVLLASTLYDNEAAKQGSITCIGQECFRVT 540

Query: 541 FFVLSGVAGLGSILSIILTVRLRPVYQMLYAGGSFRLPQSSGH 584
           FFVLSGVAGLGSIL IILTVRLRPVYQMLYAGGSFRLPQ+SGH
Sbjct: 541 FFVLSGVAGLGSILCIILTVRLRPVYQMLYAGGSFRLPQNSGH 566

BLAST of Lag0031267 vs. NCBI nr
Match: XP_022956065.1 (protein NUCLEAR FUSION DEFECTIVE 4 [Cucurbita moschata])

HSP 1 Score: 1030.8 bits (2664), Expect = 4.6e-297
Identity = 535/583 (91.77%), Postives = 557/583 (95.54%), Query Frame = 0

Query: 1   MPKLVLKGGSRPPWVGLAAAVWVQIAAGSSYNFSLYSHLLKSVLGLNQQQLTVLGVANDI 60
           M KLVLKGGSRPPWVGLAAAVWVQIAAGSSYNFSLYSHLLKSVLGLNQQQLTVLGVANDI
Sbjct: 1   MSKLVLKGGSRPPWVGLAAAVWVQIAAGSSYNFSLYSHLLKSVLGLNQQQLTVLGVANDI 60

Query: 61  GESVGLLPGLACNRFPPWAVLLVGSLCCFVGYGAIWLAVSRTVPNLPYWLLWLTHCIATN 120
           GESVGLLPGLACNRFPPWAVLLVG++CCFVGYGAIWLAVSRTVPNLPYWLLWLTHCIATN
Sbjct: 61  GESVGLLPGLACNRFPPWAVLLVGAVCCFVGYGAIWLAVSRTVPNLPYWLLWLTHCIATN 120

Query: 121 SNAWFGTAVLVTNMRNFPLSRGTVAGILKGYVGLSAAVYTVIYSMVLKKSALDLLLFLAI 180
           SNAWFGTAVLVTNMRNFPLSRGTVAGILKGYVGLSAAVYTVIYSMVL+KSALDL+LFLAI
Sbjct: 121 SNAWFGTAVLVTNMRNFPLSRGTVAGILKGYVGLSAAVYTVIYSMVLRKSALDLVLFLAI 180

Query: 181 GIPILCLALMYFVRPCTPASSEDPSERSHFLFTQAACVLLAVFLVSTTIIDATTTPSDAV 240
           GIPILCLALMYFVRPCTPAS+EDPSER+HFLFTQAACVLLAVFLVSTTI+DATTTPS+AV
Sbjct: 181 GIPILCLALMYFVRPCTPASTEDPSERNHFLFTQAACVLLAVFLVSTTILDATTTPSNAV 240

Query: 241 AYTLVAIMAILLMSPLAVPIKMTICSAKTKKLGPRVDSAEPLASGESDSSQIEPLLTPSS 300
           AYTLVAIM +LLMSPLAVPIKMTICS KTKKLGPRVDS EPLASGESDSSQIEPLLTPSS
Sbjct: 241 AYTLVAIMVVLLMSPLAVPIKMTICSVKTKKLGPRVDSTEPLASGESDSSQIEPLLTPSS 300

Query: 301 SATNLGSFYENDDASDVETLLAVGEGAIHKKRRRPKRGEDFKLREAVIKADFWLLWFLYF 360
           SATNLGSFYEND++SDVETLLAVGEGAIHKK+R+PKRGEDFKLREAVIKADFWLLWFLYF
Sbjct: 301 SATNLGSFYENDESSDVETLLAVGEGAIHKKKRKPKRGEDFKLREAVIKADFWLLWFLYF 360

Query: 361 LGVGPGITVLNNLSQIGISLGVNDTTLLLALFSFCNFIGRLGSGVISEHFVSCSIYEMLI 420
           LGVGPGITVLNNLSQIGI+LGVNDTTLLLALFSFCNF+GRLGSGVISEHFV         
Sbjct: 361 LGVGPGITVLNNLSQIGIALGVNDTTLLLALFSFCNFVGRLGSGVISEHFV--------- 420

Query: 421 GSAIVVQCRSRMIPRSLWMMFALILMSIAFLLYASALTSTLYLATGFTGISYGILYSMMV 480
                   RSRMIPRSLWMMFAL+LMS+AFLLYASA T TLY+ATG  GISYG+LY+MMV
Sbjct: 421 --------RSRMIPRSLWMMFALMLMSVAFLLYASAHTVTLYVATGLMGISYGVLYAMMV 480

Query: 481 PLASEIFGLKNFGVIFNFMQLGNPIGAVLFSVLLASTLYDNEAAKQGSITCIGQQCFRVT 540
           PLASEIFGLKNFGVIFNFMQLGNPIGAVLFSVLLASTLYDNEAAKQGSITCIGQ+CFRVT
Sbjct: 481 PLASEIFGLKNFGVIFNFMQLGNPIGAVLFSVLLASTLYDNEAAKQGSITCIGQECFRVT 540

Query: 541 FFVLSGVAGLGSILSIILTVRLRPVYQMLYAGGSFRLPQSSGH 584
           FFVLSGVAGLGSIL IILTVRLRPVYQMLYAGGSFRLPQ+SGH
Sbjct: 541 FFVLSGVAGLGSILCIILTVRLRPVYQMLYAGGSFRLPQNSGH 566

BLAST of Lag0031267 vs. NCBI nr
Match: XP_022979616.1 (protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita maxima])

HSP 1 Score: 1026.9 bits (2654), Expect = 6.6e-296
Identity = 533/583 (91.42%), Postives = 556/583 (95.37%), Query Frame = 0

Query: 1   MPKLVLKGGSRPPWVGLAAAVWVQIAAGSSYNFSLYSHLLKSVLGLNQQQLTVLGVANDI 60
           M KLVLKGGSRPPWVGLAAAVWVQIAAGSSYNFSLYSHLLKSVLGLNQQQLTVLGVANDI
Sbjct: 1   MSKLVLKGGSRPPWVGLAAAVWVQIAAGSSYNFSLYSHLLKSVLGLNQQQLTVLGVANDI 60

Query: 61  GESVGLLPGLACNRFPPWAVLLVGSLCCFVGYGAIWLAVSRTVPNLPYWLLWLTHCIATN 120
           GESVGLLPGLACNRFPPWAVLLVG++CCFVGYGAIWLAVSRTVPNLPYWLLWLTHCIATN
Sbjct: 61  GESVGLLPGLACNRFPPWAVLLVGAVCCFVGYGAIWLAVSRTVPNLPYWLLWLTHCIATN 120

Query: 121 SNAWFGTAVLVTNMRNFPLSRGTVAGILKGYVGLSAAVYTVIYSMVLKKSALDLLLFLAI 180
           SNAWFGTAVLVTNMRNFPLSRGTVAGILKGYVGLSAAVYTVIYSMVL+KSALDLLLFLAI
Sbjct: 121 SNAWFGTAVLVTNMRNFPLSRGTVAGILKGYVGLSAAVYTVIYSMVLRKSALDLLLFLAI 180

Query: 181 GIPILCLALMYFVRPCTPASSEDPSERSHFLFTQAACVLLAVFLVSTTIIDATTTPSDAV 240
           GIPILCLALMYFVRPCTPAS+EDPSE +HFLFTQAACVLLAVFLVSTTI+DATTTPS+AV
Sbjct: 181 GIPILCLALMYFVRPCTPASTEDPSEHNHFLFTQAACVLLAVFLVSTTILDATTTPSNAV 240

Query: 241 AYTLVAIMAILLMSPLAVPIKMTICSAKTKKLGPRVDSAEPLASGESDSSQIEPLLTPSS 300
           AYTLVAIM +LLMSPLAVPIKMTICS KTKKLGPRVDS EPLASGE++SSQIEPLLTPSS
Sbjct: 241 AYTLVAIMVVLLMSPLAVPIKMTICSVKTKKLGPRVDSTEPLASGENNSSQIEPLLTPSS 300

Query: 301 SATNLGSFYENDDASDVETLLAVGEGAIHKKRRRPKRGEDFKLREAVIKADFWLLWFLYF 360
           SATNLGSFYEND++SDVETLLAVGEGAIHKK+R+PKRGEDFKLREAVIKADFWLLWFLYF
Sbjct: 301 SATNLGSFYENDESSDVETLLAVGEGAIHKKKRKPKRGEDFKLREAVIKADFWLLWFLYF 360

Query: 361 LGVGPGITVLNNLSQIGISLGVNDTTLLLALFSFCNFIGRLGSGVISEHFVSCSIYEMLI 420
           LGVGPGITVLNNLSQIGISLGVNDTTLLLALFSFCNF+GRLGSGVISEHFV         
Sbjct: 361 LGVGPGITVLNNLSQIGISLGVNDTTLLLALFSFCNFVGRLGSGVISEHFV--------- 420

Query: 421 GSAIVVQCRSRMIPRSLWMMFALILMSIAFLLYASALTSTLYLATGFTGISYGILYSMMV 480
                   RSRMIPRSLWMMF+L+LMS+AFLLYASA T TLY+ATG  GISYG+LY+MMV
Sbjct: 421 --------RSRMIPRSLWMMFSLMLMSVAFLLYASAHTVTLYVATGLMGISYGVLYAMMV 480

Query: 481 PLASEIFGLKNFGVIFNFMQLGNPIGAVLFSVLLASTLYDNEAAKQGSITCIGQQCFRVT 540
           PLASEIFGLKNFGVIFNFMQLGNPIGAVLFSVLLASTLYDNEAAKQGSITCIGQ+CFRVT
Sbjct: 481 PLASEIFGLKNFGVIFNFMQLGNPIGAVLFSVLLASTLYDNEAAKQGSITCIGQECFRVT 540

Query: 541 FFVLSGVAGLGSILSIILTVRLRPVYQMLYAGGSFRLPQSSGH 584
           FFVLSGVAGLGSIL IILTVRLRPVYQMLYAGGSFRLPQ+SGH
Sbjct: 541 FFVLSGVAGLGSILCIILTVRLRPVYQMLYAGGSFRLPQNSGH 566

BLAST of Lag0031267 vs. NCBI nr
Match: XP_038896285.1 (protein NUCLEAR FUSION DEFECTIVE 4 isoform X1 [Benincasa hispida])

HSP 1 Score: 1025.4 bits (2650), Expect = 1.9e-295
Identity = 534/583 (91.60%), Postives = 553/583 (94.85%), Query Frame = 0

Query: 1   MPKLVLKGGSRPPWVGLAAAVWVQIAAGSSYNFSLYSHLLKSVLGLNQQQLTVLGVANDI 60
           MPKLVLKGGSRPPWVGLAAAVWVQIAAGSSYNFSLYSHLLKSVLGLNQQQLTVLGVANDI
Sbjct: 1   MPKLVLKGGSRPPWVGLAAAVWVQIAAGSSYNFSLYSHLLKSVLGLNQQQLTVLGVANDI 60

Query: 61  GESVGLLPGLACNRFPPWAVLLVGSLCCFVGYGAIWLAVSRTVPNLPYWLLWLTHCIATN 120
           GES+GL+PGLACNRFPPW +LL G+ CCFVGYGAIWLAVSRTVPNLPYWLLWLTHCIATN
Sbjct: 61  GESMGLIPGLACNRFPPWVILLFGAFCCFVGYGAIWLAVSRTVPNLPYWLLWLTHCIATN 120

Query: 121 SNAWFGTAVLVTNMRNFPLSRGTVAGILKGYVGLSAAVYTVIYSMVLKKSALDLLLFLAI 180
           SNAWFGTAVLVTNMRNFPLSRG+VAGILKGYVGLSAAVYTVIYSMVL+KSAL+LLLFLAI
Sbjct: 121 SNAWFGTAVLVTNMRNFPLSRGSVAGILKGYVGLSAAVYTVIYSMVLRKSALNLLLFLAI 180

Query: 181 GIPILCLALMYFVRPCTPASSEDPSERSHFLFTQAACVLLAVFLVSTTIIDATTTPSDAV 240
           GIPILCLALMYFVRPCTPASSEDPSERSHFLFTQA+CVLLAVFLVSTTI+DATTTPSDAV
Sbjct: 181 GIPILCLALMYFVRPCTPASSEDPSERSHFLFTQASCVLLAVFLVSTTIVDATTTPSDAV 240

Query: 241 AYTLVAIMAILLMSPLAVPIKMTICSAKTKKLGPRVDSAEPLASGESDSSQIEPLLTPSS 300
           AYTLVAIM I LMSPLAVPIKMTIC AKTKKLG RVDS EPLASGESDSSQIEPLLTPSS
Sbjct: 241 AYTLVAIMVIFLMSPLAVPIKMTIC-AKTKKLGSRVDSVEPLASGESDSSQIEPLLTPSS 300

Query: 301 SATNLGSFYENDDASDVETLLAVGEGAIHKKRRRPKRGEDFKLREAVIKADFWLLWFLYF 360
           SATNLGSFYENDDASDVETLLAVGEGAIHKK+RRPKRGEDFKLREAVIKADFWLLWFLYF
Sbjct: 301 SATNLGSFYENDDASDVETLLAVGEGAIHKKKRRPKRGEDFKLREAVIKADFWLLWFLYF 360

Query: 361 LGVGPGITVLNNLSQIGISLGVNDTTLLLALFSFCNFIGRLGSGVISEHFVSCSIYEMLI 420
            GVGPGITVLNNLSQIGISLGVNDTTLLLALFSFCNF+GRLGSGVISEHFV         
Sbjct: 361 FGVGPGITVLNNLSQIGISLGVNDTTLLLALFSFCNFVGRLGSGVISEHFV--------- 420

Query: 421 GSAIVVQCRSRMIPRSLWMMFALILMSIAFLLYASALTSTLYLATGFTGISYGILYSMMV 480
                   RSRMIPRSLWMMFALILM+IAFLLYASALT TLY+ATG  GISYG+LYSMMV
Sbjct: 421 --------RSRMIPRSLWMMFALILMAIAFLLYASALTGTLYVATGLIGISYGVLYSMMV 480

Query: 481 PLASEIFGLKNFGVIFNFMQLGNPIGAVLFSVLLASTLYDNEAAKQGSITCIGQQCFRVT 540
           PLASEIFGLKNFGVIFNFMQLGNPIGAVLFSVLLASTLYD EAAKQGSITCIGQQCFR+T
Sbjct: 481 PLASEIFGLKNFGVIFNFMQLGNPIGAVLFSVLLASTLYDKEAAKQGSITCIGQQCFRMT 540

Query: 541 FFVLSGVAGLGSILSIILTVRLRPVYQMLYAGGSFRLPQSSGH 584
           FF+LSGVAGLGSI+S+ILTVRLRPVYQMLY+GGSFRLPQSSGH
Sbjct: 541 FFILSGVAGLGSIVSMILTVRLRPVYQMLYSGGSFRLPQSSGH 565

BLAST of Lag0031267 vs. ExPASy Swiss-Prot
Match: F4I9E1 (Protein NUCLEAR FUSION DEFECTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=NFD4 PE=3 SV=1)

HSP 1 Score: 184.5 bits (467), Expect = 3.4e-45
Identity = 165/578 (28.55%), Postives = 276/578 (47.75%), Query Frame = 0

Query: 1   MPKLVLKGGSRPPWVGLAAAVWVQIAAGSSYNFSLYSHLLKSVLGLNQQQLTVLGVANDI 60
           +P L    G    W  L AA+W+Q + G++++FS YS  LKSVLG++Q +L  L VA+D+
Sbjct: 31  LPPLRTMFGRWRKWTVLVAAIWIQASTGTNFDFSAYSSHLKSVLGISQVRLNYLAVASDL 90

Query: 61  GESVGLLPGLACNRFPPWAVLLVGSLCCFVGYGAIWLAVSRTVPNLPYWLLWLTHCIATN 120
           G++ G   G+A   FP   VL   +   FVGYG  WL ++  +  LPY L++L   +A  
Sbjct: 91  GKAFGWSSGIALGYFPLSVVLFAAAAMGFVGYGVQWLVITNII-TLPYSLVFLCCLLAGL 150

Query: 121 SNAWFGTAVLVTNMRNFPLSRGTVAGILKGYVGLSAAVYTVIYSMVLKKSA-LDLLLFLA 180
           S  WF TA  +  +R+FP +R     +   + G+SAA+Y++ ++ +   S+ L LLL   
Sbjct: 151 SICWFNTACFILCIRHFPNNRALALSLTVSFNGISAALYSLAFNAINPSSSNLYLLLNSL 210

Query: 181 IGIPILCLALMYFVRPCTPASSEDPSERSHFLFTQAACVLLAVFLVSTTIIDATTTPSDA 240
           + + +   AL   +   +  ++ D   R H         +LAV      ++ +++T S  
Sbjct: 211 VPLVVSFAALYPVLTKPSLDTTPDYDSRRHDSHVFTILNVLAVITSFHLLLSSSSTSSAR 270

Query: 241 VAYTLVAIMAILLMSPLAVPIKMTICSAKTKKLGPRVDSAEPLASGESDSSQIEPLLTPS 300
           + +       +LL+ PL  P+   +  A+   L P +++     S       I+ L    
Sbjct: 271 LNFIGA---VVLLVFPLCAPL---LVYARDYFL-PVINARLNHESSGYVMLNIDELKNQK 330

Query: 301 SSATNLGSFYENDDASDVETLLAVGEGAIHKKRRRPKRGEDFKLREAVIKADFWLLWFLY 360
           +S ++   +     A +  T+               + G++   R  + + +FWL +  Y
Sbjct: 331 TSVSSKTGYEHMGTAKEGNTV---------------RLGDEHSFRLLISRLEFWLYYIAY 390

Query: 361 FLGVGPGITVLNNLSQIGISLGVNDTTLLLALFSFCNFIGRLGSGVISEHFVSCSIYEML 420
           F G   G+   NNL QI  SLG N TT L+ ++S  +F GR                  L
Sbjct: 391 FCGGTIGLVYSNNLGQIAQSLGQNSTT-LVTIYSSFSFFGR------------------L 450

Query: 421 IGSAIVVQCRSRMIPRSLWMMFALILMSIAFLLYA--SALTSTLYLATGFTGISYGILYS 480
           + +A     +   + R+ W   AL+   IAF L A  S+  + L  AT   G+S G +++
Sbjct: 451 LSAAPDFMHKRFRLTRTGWFAIALLPTPIAFFLLAVSSSQQTALQTATALIGLSSGFIFA 510

Query: 481 MMVPLASEIFGLKNFGVIFNFMQLGNPIGAVLFSVLLASTLYDNEAAK------QGSITC 540
             V + S++FG  + GV  N +    PIG++L+  + AS +Y+  A+         SI C
Sbjct: 511 AAVSITSDLFGPNSVGVNHNILITNIPIGSLLYGYIAAS-IYEANASPDITPIVSDSIVC 565

Query: 541 IGQQCFRVTFFVLSGVAGLGSILSIILTVRLRPVYQML 570
           IG+ C+  TF     ++ LG + S+ L +R +PVY  L
Sbjct: 571 IGRDCYFKTFVFWGCLSILGVVSSLSLYIRTKPVYHRL 565

BLAST of Lag0031267 vs. ExPASy Swiss-Prot
Match: Q4IM48 (Probable transporter MCH1 OS=Gibberella zeae (strain ATCC MYA-4620 / CBS 123657 / FGSC 9075 / NRRL 31084 / PH-1) OX=229533 GN=MCH1 PE=3 SV=1)

HSP 1 Score: 47.4 bits (111), Expect = 6.6e-04
Identity = 53/214 (24.77%), Postives = 95/214 (44.39%), Query Frame = 0

Query: 353 WLLWFLYFLGVGPGITVLNNLSQI----------GISLGVNDTTLLLALFSFCNFIGRLG 412
           W     + L VGPG   +NNL  I          G S   +  T  +++F   N   R+ 
Sbjct: 331 WPFALAFLLIVGPGEAFINNLGTIIGTLTPPEMEGWSHRTSAAT-HVSIFGITNTASRIF 390

Query: 413 SGVISEHFVSCSIYEMLIGSAIVVQCRSRM-IPRSLWMMFALILMSIAFLLYASALTST- 472
            G +++        + + G +      SR  I R  +M F   ++SI  L+ AS L    
Sbjct: 391 IGTLTDLLAPYPHTQHVQGPSTRSAVSSRFSISRVAFMAFFASMLSIGLLILASGLVQNH 450

Query: 473 ---LYLATGFTGISYGILYSMMVPLASEIFGLKNFGVIFNFMQLGNPIGAVLFSVLLAST 532
               +L +G  G  YG ++S+   + + I+G++NF   +  + +    G+  + ++ ++T
Sbjct: 451 AERFWLVSGLVGAGYGAIFSLTPLMVTIIWGVENFATNYGLIGMLPAAGSTFWGLVYSAT 510

Query: 533 LYDNEAAK-----QGS----ITCIGQQCFRVTFF 543
            Y N A K     +GS    + C G+QC+  T++
Sbjct: 511 -YQNGANKSKAGPEGSDRDDLFCYGEQCYAPTYW 542

BLAST of Lag0031267 vs. ExPASy TrEMBL
Match: A0A6J1GWT3 (protein NUCLEAR FUSION DEFECTIVE 4 OS=Cucurbita moschata OX=3662 GN=LOC111457872 PE=4 SV=1)

HSP 1 Score: 1030.8 bits (2664), Expect = 2.2e-297
Identity = 535/583 (91.77%), Postives = 557/583 (95.54%), Query Frame = 0

Query: 1   MPKLVLKGGSRPPWVGLAAAVWVQIAAGSSYNFSLYSHLLKSVLGLNQQQLTVLGVANDI 60
           M KLVLKGGSRPPWVGLAAAVWVQIAAGSSYNFSLYSHLLKSVLGLNQQQLTVLGVANDI
Sbjct: 1   MSKLVLKGGSRPPWVGLAAAVWVQIAAGSSYNFSLYSHLLKSVLGLNQQQLTVLGVANDI 60

Query: 61  GESVGLLPGLACNRFPPWAVLLVGSLCCFVGYGAIWLAVSRTVPNLPYWLLWLTHCIATN 120
           GESVGLLPGLACNRFPPWAVLLVG++CCFVGYGAIWLAVSRTVPNLPYWLLWLTHCIATN
Sbjct: 61  GESVGLLPGLACNRFPPWAVLLVGAVCCFVGYGAIWLAVSRTVPNLPYWLLWLTHCIATN 120

Query: 121 SNAWFGTAVLVTNMRNFPLSRGTVAGILKGYVGLSAAVYTVIYSMVLKKSALDLLLFLAI 180
           SNAWFGTAVLVTNMRNFPLSRGTVAGILKGYVGLSAAVYTVIYSMVL+KSALDL+LFLAI
Sbjct: 121 SNAWFGTAVLVTNMRNFPLSRGTVAGILKGYVGLSAAVYTVIYSMVLRKSALDLVLFLAI 180

Query: 181 GIPILCLALMYFVRPCTPASSEDPSERSHFLFTQAACVLLAVFLVSTTIIDATTTPSDAV 240
           GIPILCLALMYFVRPCTPAS+EDPSER+HFLFTQAACVLLAVFLVSTTI+DATTTPS+AV
Sbjct: 181 GIPILCLALMYFVRPCTPASTEDPSERNHFLFTQAACVLLAVFLVSTTILDATTTPSNAV 240

Query: 241 AYTLVAIMAILLMSPLAVPIKMTICSAKTKKLGPRVDSAEPLASGESDSSQIEPLLTPSS 300
           AYTLVAIM +LLMSPLAVPIKMTICS KTKKLGPRVDS EPLASGESDSSQIEPLLTPSS
Sbjct: 241 AYTLVAIMVVLLMSPLAVPIKMTICSVKTKKLGPRVDSTEPLASGESDSSQIEPLLTPSS 300

Query: 301 SATNLGSFYENDDASDVETLLAVGEGAIHKKRRRPKRGEDFKLREAVIKADFWLLWFLYF 360
           SATNLGSFYEND++SDVETLLAVGEGAIHKK+R+PKRGEDFKLREAVIKADFWLLWFLYF
Sbjct: 301 SATNLGSFYENDESSDVETLLAVGEGAIHKKKRKPKRGEDFKLREAVIKADFWLLWFLYF 360

Query: 361 LGVGPGITVLNNLSQIGISLGVNDTTLLLALFSFCNFIGRLGSGVISEHFVSCSIYEMLI 420
           LGVGPGITVLNNLSQIGI+LGVNDTTLLLALFSFCNF+GRLGSGVISEHFV         
Sbjct: 361 LGVGPGITVLNNLSQIGIALGVNDTTLLLALFSFCNFVGRLGSGVISEHFV--------- 420

Query: 421 GSAIVVQCRSRMIPRSLWMMFALILMSIAFLLYASALTSTLYLATGFTGISYGILYSMMV 480
                   RSRMIPRSLWMMFAL+LMS+AFLLYASA T TLY+ATG  GISYG+LY+MMV
Sbjct: 421 --------RSRMIPRSLWMMFALMLMSVAFLLYASAHTVTLYVATGLMGISYGVLYAMMV 480

Query: 481 PLASEIFGLKNFGVIFNFMQLGNPIGAVLFSVLLASTLYDNEAAKQGSITCIGQQCFRVT 540
           PLASEIFGLKNFGVIFNFMQLGNPIGAVLFSVLLASTLYDNEAAKQGSITCIGQ+CFRVT
Sbjct: 481 PLASEIFGLKNFGVIFNFMQLGNPIGAVLFSVLLASTLYDNEAAKQGSITCIGQECFRVT 540

Query: 541 FFVLSGVAGLGSILSIILTVRLRPVYQMLYAGGSFRLPQSSGH 584
           FFVLSGVAGLGSIL IILTVRLRPVYQMLYAGGSFRLPQ+SGH
Sbjct: 541 FFVLSGVAGLGSILCIILTVRLRPVYQMLYAGGSFRLPQNSGH 566

BLAST of Lag0031267 vs. ExPASy TrEMBL
Match: A0A6J1IRA6 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita maxima OX=3661 GN=LOC111479292 PE=4 SV=1)

HSP 1 Score: 1026.9 bits (2654), Expect = 3.2e-296
Identity = 533/583 (91.42%), Postives = 556/583 (95.37%), Query Frame = 0

Query: 1   MPKLVLKGGSRPPWVGLAAAVWVQIAAGSSYNFSLYSHLLKSVLGLNQQQLTVLGVANDI 60
           M KLVLKGGSRPPWVGLAAAVWVQIAAGSSYNFSLYSHLLKSVLGLNQQQLTVLGVANDI
Sbjct: 1   MSKLVLKGGSRPPWVGLAAAVWVQIAAGSSYNFSLYSHLLKSVLGLNQQQLTVLGVANDI 60

Query: 61  GESVGLLPGLACNRFPPWAVLLVGSLCCFVGYGAIWLAVSRTVPNLPYWLLWLTHCIATN 120
           GESVGLLPGLACNRFPPWAVLLVG++CCFVGYGAIWLAVSRTVPNLPYWLLWLTHCIATN
Sbjct: 61  GESVGLLPGLACNRFPPWAVLLVGAVCCFVGYGAIWLAVSRTVPNLPYWLLWLTHCIATN 120

Query: 121 SNAWFGTAVLVTNMRNFPLSRGTVAGILKGYVGLSAAVYTVIYSMVLKKSALDLLLFLAI 180
           SNAWFGTAVLVTNMRNFPLSRGTVAGILKGYVGLSAAVYTVIYSMVL+KSALDLLLFLAI
Sbjct: 121 SNAWFGTAVLVTNMRNFPLSRGTVAGILKGYVGLSAAVYTVIYSMVLRKSALDLLLFLAI 180

Query: 181 GIPILCLALMYFVRPCTPASSEDPSERSHFLFTQAACVLLAVFLVSTTIIDATTTPSDAV 240
           GIPILCLALMYFVRPCTPAS+EDPSE +HFLFTQAACVLLAVFLVSTTI+DATTTPS+AV
Sbjct: 181 GIPILCLALMYFVRPCTPASTEDPSEHNHFLFTQAACVLLAVFLVSTTILDATTTPSNAV 240

Query: 241 AYTLVAIMAILLMSPLAVPIKMTICSAKTKKLGPRVDSAEPLASGESDSSQIEPLLTPSS 300
           AYTLVAIM +LLMSPLAVPIKMTICS KTKKLGPRVDS EPLASGE++SSQIEPLLTPSS
Sbjct: 241 AYTLVAIMVVLLMSPLAVPIKMTICSVKTKKLGPRVDSTEPLASGENNSSQIEPLLTPSS 300

Query: 301 SATNLGSFYENDDASDVETLLAVGEGAIHKKRRRPKRGEDFKLREAVIKADFWLLWFLYF 360
           SATNLGSFYEND++SDVETLLAVGEGAIHKK+R+PKRGEDFKLREAVIKADFWLLWFLYF
Sbjct: 301 SATNLGSFYENDESSDVETLLAVGEGAIHKKKRKPKRGEDFKLREAVIKADFWLLWFLYF 360

Query: 361 LGVGPGITVLNNLSQIGISLGVNDTTLLLALFSFCNFIGRLGSGVISEHFVSCSIYEMLI 420
           LGVGPGITVLNNLSQIGISLGVNDTTLLLALFSFCNF+GRLGSGVISEHFV         
Sbjct: 361 LGVGPGITVLNNLSQIGISLGVNDTTLLLALFSFCNFVGRLGSGVISEHFV--------- 420

Query: 421 GSAIVVQCRSRMIPRSLWMMFALILMSIAFLLYASALTSTLYLATGFTGISYGILYSMMV 480
                   RSRMIPRSLWMMF+L+LMS+AFLLYASA T TLY+ATG  GISYG+LY+MMV
Sbjct: 421 --------RSRMIPRSLWMMFSLMLMSVAFLLYASAHTVTLYVATGLMGISYGVLYAMMV 480

Query: 481 PLASEIFGLKNFGVIFNFMQLGNPIGAVLFSVLLASTLYDNEAAKQGSITCIGQQCFRVT 540
           PLASEIFGLKNFGVIFNFMQLGNPIGAVLFSVLLASTLYDNEAAKQGSITCIGQ+CFRVT
Sbjct: 481 PLASEIFGLKNFGVIFNFMQLGNPIGAVLFSVLLASTLYDNEAAKQGSITCIGQECFRVT 540

Query: 541 FFVLSGVAGLGSILSIILTVRLRPVYQMLYAGGSFRLPQSSGH 584
           FFVLSGVAGLGSIL IILTVRLRPVYQMLYAGGSFRLPQ+SGH
Sbjct: 541 FFVLSGVAGLGSILCIILTVRLRPVYQMLYAGGSFRLPQNSGH 566

BLAST of Lag0031267 vs. ExPASy TrEMBL
Match: A0A0A0KTP3 (Nodulin-like domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G006300 PE=4 SV=1)

HSP 1 Score: 1017.3 bits (2629), Expect = 2.5e-293
Identity = 524/583 (89.88%), Postives = 551/583 (94.51%), Query Frame = 0

Query: 1   MPKLVLKGGSRPPWVGLAAAVWVQIAAGSSYNFSLYSHLLKSVLGLNQQQLTVLGVANDI 60
           MPKLVLKGG+RPPWVGLAAAVWVQIA GSSYNFSLYSHLLKSVLGLNQQQLTVLGVANDI
Sbjct: 1   MPKLVLKGGTRPPWVGLAAAVWVQIAGGSSYNFSLYSHLLKSVLGLNQQQLTVLGVANDI 60

Query: 61  GESVGLLPGLACNRFPPWAVLLVGSLCCFVGYGAIWLAVSRTVPNLPYWLLWLTHCIATN 120
           GES+GL+PGLACN+FPPW +LL G+ CCF+GYGAIWLAVSRTVPNLPYWLLWLTHC+ATN
Sbjct: 61  GESMGLIPGLACNKFPPWVILLFGAFCCFIGYGAIWLAVSRTVPNLPYWLLWLTHCVATN 120

Query: 121 SNAWFGTAVLVTNMRNFPLSRGTVAGILKGYVGLSAAVYTVIYSMVLKKSALDLLLFLAI 180
           SNAWFGTAVLVTNMRNFPLSRGTVAGILKGYVGLSAAVYTVIYS+VL+KSAL+LLLFLAI
Sbjct: 121 SNAWFGTAVLVTNMRNFPLSRGTVAGILKGYVGLSAAVYTVIYSLVLRKSALNLLLFLAI 180

Query: 181 GIPILCLALMYFVRPCTPASSEDPSERSHFLFTQAACVLLAVFLVSTTIIDATTTPSDAV 240
           GIPILCLA+MYFVRPCTPASSEDPSER+HFLFTQAACVLL +FLVSTTI+DATTTPSDAV
Sbjct: 181 GIPILCLAMMYFVRPCTPASSEDPSERAHFLFTQAACVLLGLFLVSTTILDATTTPSDAV 240

Query: 241 AYTLVAIMAILLMSPLAVPIKMTICSAKTKKLGPRVDSAEPLASGESDSSQIEPLLTPSS 300
            YTLVAIM ILLMSPLAVPIKMTIC A+TK LGPRVDS+EPLASGESDSSQIEPLLTPSS
Sbjct: 241 GYTLVAIMVILLMSPLAVPIKMTIC-ARTKTLGPRVDSSEPLASGESDSSQIEPLLTPSS 300

Query: 301 SATNLGSFYENDDASDVETLLAVGEGAIHKKRRRPKRGEDFKLREAVIKADFWLLWFLYF 360
           SATNLGSFYENDDASDVETLLAVGEGAIHKK+RRPKRGEDFKLREAVIKADFWLLWFLYF
Sbjct: 301 SATNLGSFYENDDASDVETLLAVGEGAIHKKKRRPKRGEDFKLREAVIKADFWLLWFLYF 360

Query: 361 LGVGPGITVLNNLSQIGISLGVNDTTLLLALFSFCNFIGRLGSGVISEHFVSCSIYEMLI 420
           LGVGPGITVLNNLSQIGISLG+ND TLLLALFSFCNF+GRLGSGVISEHFV         
Sbjct: 361 LGVGPGITVLNNLSQIGISLGINDATLLLALFSFCNFVGRLGSGVISEHFV--------- 420

Query: 421 GSAIVVQCRSRMIPRSLWMMFALILMSIAFLLYASALTSTLYLATGFTGISYGILYSMMV 480
                   RSRMIPRSLWMMFAL+LMSIAFLLYASALT TLY+ATG TGISYG+LYSMMV
Sbjct: 421 --------RSRMIPRSLWMMFALVLMSIAFLLYASALTITLYIATGLTGISYGVLYSMMV 480

Query: 481 PLASEIFGLKNFGVIFNFMQLGNPIGAVLFSVLLASTLYDNEAAKQGSITCIGQQCFRVT 540
           PLASEIFGLKNFGVIFNFMQLGNPIGAVLFSVLL STLYD EAAKQGSITCIGQQCFR T
Sbjct: 481 PLASEIFGLKNFGVIFNFMQLGNPIGAVLFSVLLTSTLYDIEAAKQGSITCIGQQCFRTT 540

Query: 541 FFVLSGVAGLGSILSIILTVRLRPVYQMLYAGGSFRLPQSSGH 584
           FF+LSGVAGLGSI+S+ILT+RLRPVYQMLYAGGSFRLPQSSGH
Sbjct: 541 FFILSGVAGLGSIVSLILTIRLRPVYQMLYAGGSFRLPQSSGH 565

BLAST of Lag0031267 vs. ExPASy TrEMBL
Match: A0A5D3E0Y7 (Protein NUCLEAR FUSION DEFECTIVE 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold655G002010 PE=4 SV=1)

HSP 1 Score: 1014.2 bits (2621), Expect = 2.2e-292
Identity = 524/583 (89.88%), Postives = 548/583 (94.00%), Query Frame = 0

Query: 1   MPKLVLKGGSRPPWVGLAAAVWVQIAAGSSYNFSLYSHLLKSVLGLNQQQLTVLGVANDI 60
           MPKLVLKGG+RPPWVGLAAAVWVQIA GSSYNFSLYSHLLKSVLGLNQQQLTVLGVANDI
Sbjct: 1   MPKLVLKGGTRPPWVGLAAAVWVQIAGGSSYNFSLYSHLLKSVLGLNQQQLTVLGVANDI 60

Query: 61  GESVGLLPGLACNRFPPWAVLLVGSLCCFVGYGAIWLAVSRTVPNLPYWLLWLTHCIATN 120
           GES+GL+PGLACNRFPPW +LL G+ CCF+GYGAIWLAVSRTVPNLPYWLLWLTHCIATN
Sbjct: 61  GESMGLVPGLACNRFPPWVILLFGAFCCFIGYGAIWLAVSRTVPNLPYWLLWLTHCIATN 120

Query: 121 SNAWFGTAVLVTNMRNFPLSRGTVAGILKGYVGLSAAVYTVIYSMVLKKSALDLLLFLAI 180
           SNAWFGTAVLVTNMRNFPLSRGTVAGILKGYVGLSAAVYTVIYS+VLKKSAL+LLLFL I
Sbjct: 121 SNAWFGTAVLVTNMRNFPLSRGTVAGILKGYVGLSAAVYTVIYSLVLKKSALNLLLFLTI 180

Query: 181 GIPILCLALMYFVRPCTPASSEDPSERSHFLFTQAACVLLAVFLVSTTIIDATTTPSDAV 240
           GIPILCLALMYFVRPCTPAS+EDPSER+HFLFTQAACVLL +FLVSTTI+DATTTPSDAV
Sbjct: 181 GIPILCLALMYFVRPCTPASTEDPSERAHFLFTQAACVLLGLFLVSTTILDATTTPSDAV 240

Query: 241 AYTLVAIMAILLMSPLAVPIKMTICSAKTKKLGPRVDSAEPLASGESDSSQIEPLLTPSS 300
            YTLVAIM ILLMSPLAVPIKMTIC AKTK +GPRVDS+EPLASG SDSSQIEPLLTPSS
Sbjct: 241 GYTLVAIMVILLMSPLAVPIKMTIC-AKTKTVGPRVDSSEPLASGGSDSSQIEPLLTPSS 300

Query: 301 SATNLGSFYENDDASDVETLLAVGEGAIHKKRRRPKRGEDFKLREAVIKADFWLLWFLYF 360
           SATNLGSFYENDDASDVETLLAVGEGAIHKK+RRPKRGEDFKLREAVIKADFWLLWFLYF
Sbjct: 301 SATNLGSFYENDDASDVETLLAVGEGAIHKKKRRPKRGEDFKLREAVIKADFWLLWFLYF 360

Query: 361 LGVGPGITVLNNLSQIGISLGVNDTTLLLALFSFCNFIGRLGSGVISEHFVSCSIYEMLI 420
           LGVGPGITVLNNLSQIGISLG+NDTTLLLALFSFCNF+GRLGSGVISEHFV         
Sbjct: 361 LGVGPGITVLNNLSQIGISLGINDTTLLLALFSFCNFVGRLGSGVISEHFV--------- 420

Query: 421 GSAIVVQCRSRMIPRSLWMMFALILMSIAFLLYASALTSTLYLATGFTGISYGILYSMMV 480
                   RSRMIPRSLWMMFAL+LMSIAFLLYAS LT TLY+ATG TGISYG+LYSMMV
Sbjct: 421 --------RSRMIPRSLWMMFALVLMSIAFLLYASGLTITLYIATGLTGISYGVLYSMMV 480

Query: 481 PLASEIFGLKNFGVIFNFMQLGNPIGAVLFSVLLASTLYDNEAAKQGSITCIGQQCFRVT 540
           PLASEIFGLKNFGVIFNFMQLGNPIGAVLFSVLL STLYD EAA QGSITCIGQQCFR T
Sbjct: 481 PLASEIFGLKNFGVIFNFMQLGNPIGAVLFSVLLTSTLYDKEAAMQGSITCIGQQCFRTT 540

Query: 541 FFVLSGVAGLGSILSIILTVRLRPVYQMLYAGGSFRLPQSSGH 584
           FF+LSGVAGLGSI+S+ILT+RLRPVYQMLYAGGSFRLPQSSGH
Sbjct: 541 FFILSGVAGLGSIVSLILTIRLRPVYQMLYAGGSFRLPQSSGH 565

BLAST of Lag0031267 vs. ExPASy TrEMBL
Match: A0A1S3BY60 (LOW QUALITY PROTEIN: protein NUCLEAR FUSION DEFECTIVE 4 OS=Cucumis melo OX=3656 GN=LOC103494674 PE=4 SV=1)

HSP 1 Score: 1012.7 bits (2617), Expect = 6.3e-292
Identity = 524/583 (89.88%), Postives = 547/583 (93.83%), Query Frame = 0

Query: 1   MPKLVLKGGSRPPWVGLAAAVWVQIAAGSSYNFSLYSHLLKSVLGLNQQQLTVLGVANDI 60
           MPKLVLKGG+RPPWVGLAAAVWVQIA GSSYNFSLYSHLLKSVLGLNQQQLTVLGVANDI
Sbjct: 1   MPKLVLKGGTRPPWVGLAAAVWVQIAGGSSYNFSLYSHLLKSVLGLNQQQLTVLGVANDI 60

Query: 61  GESVGLLPGLACNRFPPWAVLLVGSLCCFVGYGAIWLAVSRTVPNLPYWLLWLTHCIATN 120
           GES+GL+PGLACNRFPPW +LL G+ CCF+GYGAIWLAVSRTVPNLPYWLLWLTHCIATN
Sbjct: 61  GESMGLVPGLACNRFPPWVILLFGAFCCFIGYGAIWLAVSRTVPNLPYWLLWLTHCIATN 120

Query: 121 SNAWFGTAVLVTNMRNFPLSRGTVAGILKGYVGLSAAVYTVIYSMVLKKSALDLLLFLAI 180
           SNAWFGTAVLVTNMRNFPLSRGTVAGILKGYVGLSAAVYTVIYS+VLKKSAL+LLLFL I
Sbjct: 121 SNAWFGTAVLVTNMRNFPLSRGTVAGILKGYVGLSAAVYTVIYSLVLKKSALNLLLFLTI 180

Query: 181 GIPILCLALMYFVRPCTPASSEDPSERSHFLFTQAACVLLAVFLVSTTIIDATTTPSDAV 240
           GIPILCLALMYFVRPCTPAS+EDPSER+HFLFTQAACVLL  FLVSTTI+DATTTPSDAV
Sbjct: 181 GIPILCLALMYFVRPCTPASTEDPSERAHFLFTQAACVLLXSFLVSTTILDATTTPSDAV 240

Query: 241 AYTLVAIMAILLMSPLAVPIKMTICSAKTKKLGPRVDSAEPLASGESDSSQIEPLLTPSS 300
            YTLVAIM ILLMSPLAVPIKMTIC AKTK +GPRVDS+EPLASG SDSSQIEPLLTPSS
Sbjct: 241 GYTLVAIMVILLMSPLAVPIKMTIC-AKTKTVGPRVDSSEPLASGGSDSSQIEPLLTPSS 300

Query: 301 SATNLGSFYENDDASDVETLLAVGEGAIHKKRRRPKRGEDFKLREAVIKADFWLLWFLYF 360
           SATNLGSFYENDDASDVETLLAVGEGAIHKK+RRPKRGEDFKLREAVIKADFWLLWFLYF
Sbjct: 301 SATNLGSFYENDDASDVETLLAVGEGAIHKKKRRPKRGEDFKLREAVIKADFWLLWFLYF 360

Query: 361 LGVGPGITVLNNLSQIGISLGVNDTTLLLALFSFCNFIGRLGSGVISEHFVSCSIYEMLI 420
           LGVGPGITVLNNLSQIGISLG+NDTTLLLALFSFCNF+GRLGSGVISEHFV         
Sbjct: 361 LGVGPGITVLNNLSQIGISLGINDTTLLLALFSFCNFVGRLGSGVISEHFV--------- 420

Query: 421 GSAIVVQCRSRMIPRSLWMMFALILMSIAFLLYASALTSTLYLATGFTGISYGILYSMMV 480
                   RSRMIPRSLWMMFAL+LMSIAFLLYAS LT TLY+ATG TGISYG+LYSMMV
Sbjct: 421 --------RSRMIPRSLWMMFALVLMSIAFLLYASGLTITLYIATGLTGISYGVLYSMMV 480

Query: 481 PLASEIFGLKNFGVIFNFMQLGNPIGAVLFSVLLASTLYDNEAAKQGSITCIGQQCFRVT 540
           PLASEIFGLKNFGVIFNFMQLGNPIGAVLFSVLL STLYD EAA QGSITCIGQQCFR T
Sbjct: 481 PLASEIFGLKNFGVIFNFMQLGNPIGAVLFSVLLTSTLYDKEAAMQGSITCIGQQCFRTT 540

Query: 541 FFVLSGVAGLGSILSIILTVRLRPVYQMLYAGGSFRLPQSSGH 584
           FF+LSGVAGLGSI+S+ILT+RLRPVYQMLYAGGSFRLPQSSGH
Sbjct: 541 FFILSGVAGLGSIVSLILTIRLRPVYQMLYAGGSFRLPQSSGH 565

BLAST of Lag0031267 vs. TAIR 10
Match: AT1G80530.1 (Major facilitator superfamily protein )

HSP 1 Score: 650.6 bits (1677), Expect = 1.2e-186
Identity = 340/586 (58.02%), Postives = 436/586 (74.40%), Query Frame = 0

Query: 1   MPKLVLKGGSRPPWVGLAAAVWVQIAAGSSYNFSLYSHLLKSVLGLNQQQLTVLGVANDI 60
           M KL  K GSRPPWVGLAAA WVQ++AGS   F LYS  LKSVLG +QQQ+T+LGVA D+
Sbjct: 1   MTKLAEKSGSRPPWVGLAAAAWVQVSAGSGSTFPLYSSALKSVLGFSQQQVTILGVACDL 60

Query: 61  GESVGLLPGLACNRFPPWAVLLVGSLCCFVGYGAIWLAVSRTVPNLPYWLLWLTHCIATN 120
           GE++GLLPG A N+ PPW++LL+G+  CF+G+G +WL+VS+ V  LP+WLL++   +ATN
Sbjct: 61  GENMGLLPGYASNKLPPWSMLLIGASSCFLGFGVLWLSVSQIVLGLPFWLLFVALALATN 120

Query: 121 SNAWFGTAVLVTNMRNFPLSRGTVAGILKGYVGLSAAVYTVIYSMVLKKSALDLLLFLAI 180
           SN+WFGTA LVTNMRNFP+SRG VAG+LKGY+G+S A +TV++SMVL  SA+DLLLFL +
Sbjct: 121 SNSWFGTASLVTNMRNFPMSRGPVAGLLKGYIGISGAAFTVLFSMVLHHSAMDLLLFLTV 180

Query: 181 GIPILCLALMYFVRPCTPASSEDPSERSHFLFTQAACVLLAVFLVSTTIIDATTTPSDAV 240
           GIP++CL +MYF+RPC PA+ EDPSE  +F F     +L A +LV TT++         +
Sbjct: 181 GIPVICLTVMYFIRPCIPATGEDPSEPMYFAFLLVTSILFAAYLVVTTVLSEVFILPSIL 240

Query: 241 AYTLVAIMAILLMSPLAVPIKMTI--CSAKTKKLGPRVDSAEPLASGESDSSQIEPLLTP 300
            Y LVAIM +LL+SPLAVPIKMT+   +AK+  LG    S++ LA  + + +  EPLLTP
Sbjct: 241 KYVLVAIMVLLLLSPLAVPIKMTLFRSNAKSSPLG----SSDNLA--KEEGTHEEPLLTP 300

Query: 301 SSSATNLGSFYENDDASDVETLLAVGEGAIHKKRRRPKRGEDFKLREAVIKADFWLLWFL 360
           S+SA+NLG  +E DD SD+E LLA  EGA+ KK+R+P+RGEDFK  +  +KADFWLLWF+
Sbjct: 301 STSASNLGPIFEGDDESDMEILLAEAEGAV-KKKRKPRRGEDFKFGQVFVKADFWLLWFV 360

Query: 361 YFLGVGPGITVLNNLSQIGISLGVNDTTLLLALFSFCNFIGRLGSGVISEHFVSCSIYEM 420
           YFLG+G G+TV NNL+QIG + G+ DTT+LL LFSF NFIGRL SG ISEHFV       
Sbjct: 361 YFLGMGSGVTVSNNLAQIGFAFGIKDTTILLCLFSFFNFIGRLASGAISEHFV------- 420

Query: 421 LIGSAIVVQCRSRMIPRSLWMMFALILMSIAFLLYASALTSTLYLATGFTGISYGILYSM 480
                     RSR +PR+LWM  A ++M   FLL+A A+  T+Y+AT   GI  G  + +
Sbjct: 421 ----------RSRTLPRTLWMGAAQLVMVFTFLLFAMAIDHTIYVATALIGICMGFQF-L 480

Query: 481 MVPLASEIFGLKNFGVIFNFMQLGNPIGAVLFSVLLASTLYDNEAAKQGSITCIGQQCFR 540
            +   SE+FGL++FG+ FNF+ LGNP+GA +FS +LA  +YD EA KQG +TCIG  CFR
Sbjct: 481 SIATISELFGLRHFGINFNFILLGNPLGATIFSAILAGYIYDKEADKQGKMTCIGPDCFR 540

Query: 541 VTFFVLSGVAGLGSILSIILTVRLRPVYQMLYAGGSFRL-PQSSGH 584
           VTF VL+GV GLG++LSIILTVR+RPVYQ LYA GSFRL PQS+GH
Sbjct: 541 VTFLVLAGVCGLGTLLSIILTVRIRPVYQALYASGSFRLQPQSTGH 561

BLAST of Lag0031267 vs. TAIR 10
Match: AT2G16660.1 (Major facilitator superfamily protein )

HSP 1 Score: 332.4 bits (851), Expect = 7.2e-91
Identity = 209/573 (36.47%), Postives = 315/573 (54.97%), Query Frame = 0

Query: 6   LKGGSRPPWVGLAAAVWVQIAAGSSYNFSLYSHLLKSVLGLNQQQLTVLGVANDIGESVG 65
           +   S   W+G   AVWVQ  +G++Y FS YS  LKS++ LNQ +L  L VA D+G++ G
Sbjct: 7   ISSSSALKWLGFVTAVWVQSISGNNYTFSNYSGALKSLMNLNQLELNNLSVAKDVGKAFG 66

Query: 66  LLPGLACNRFPPWAVLLVGSLCCFVGYGAIWLAVSRTVPNLPYWLLWLTHCIATNSNAWF 125
           +L GLA +R P   +LL+G     +GYG  WL VSRT+  +PYW + +  C+  NS  W 
Sbjct: 67  ILAGLASDRLPTPVILLIGCFEGLLGYGVQWLVVSRTIQPIPYWQMCIFLCMGGNSTTWM 126

Query: 126 GTAVLVTNMRNFPLSRGTVAGILKGYVGLSAAVYTVIYSMVLKKSALDLLLFLAIGIPIL 185
            TAVLVT +RNF  +RG V+GILKGYVGLS A++T + + +        L+ LA+    +
Sbjct: 127 NTAVLVTCIRNFRRNRGPVSGILKGYVGLSTAIFTDLCTALFSNDPASFLVLLAVVPFAV 186

Query: 186 CLALMYFVRPCTPASS--EDPSERSHFLFTQAACVLLAVFLVSTTIIDATTTPSDAVAYT 245
           CL  ++F+R   PASS  E+  E  +F       V++AV+L S  II   T      +  
Sbjct: 187 CLTAVFFLREIPPASSAAEENEETRYFTIFNIVAVVVAVYLQSYDIIGIKT---GVFSVA 246

Query: 246 LVAIMAILLMSPLAVPIKMTICSAKTKKLGPRVDSAEPLASGESDSSQ---IEPLLTPSS 305
             +I+  LL SP+A+P    I S               L  GE D  +    EPLL    
Sbjct: 247 FASILLFLLASPIAIPFHSFIKS---------------LNYGEQDDLEGRIQEPLLRSEI 306

Query: 306 SATNLGSFYENDDASDVETLLAVGEGAIHKKRRRPKRGEDFKLREAVIKADFWLLWFLYF 365
           +A             +V  + AV      KKR++P  GED  + EAV+  DFW+L+  + 
Sbjct: 307 AAAE----------KEVIVVAAVAAEEEEKKRKKPVLGEDHTIMEAVLTVDFWVLFVSFL 366

Query: 366 LGVGPGITVLNNLSQIGISLGVNDTTLLLALFSFCNFIGRLGSGVISEHFVSCSIYEMLI 425
            GVG G+ V+NN+ QIG++LG  + ++ +++ S   F GR+ SG +SE+F+         
Sbjct: 367 CGVGTGLAVMNNMGQIGLALGYTNVSIFVSMTSIWGFFGRILSGTLSEYFL--------- 426

Query: 426 GSAIVVQCRSRMIPRSLWMMFALILMSIAFLLYASALTSTLYLATGFTGISYGILYSMMV 485
                   +    PR LW   + ILM++ ++L A A+ ++LY+ +   G+ YG+  ++ V
Sbjct: 427 --------KKAGTPRPLWNAASQILMAVGYILMALAVPNSLYIGSMVVGVCYGVRLAITV 486

Query: 486 PLASEIFGLKNFGVIFNFMQLGNPIGAVLFSVLLASTLYDNEAAKQ--GSITCIGQQCFR 545
           P ASE+FGLK +G+I+N + L  P+G+ LFS LLA  LYD EA     G  TC+G  C+R
Sbjct: 487 PTASELFGLKYYGLIYNILVLNLPLGSFLFSGLLAGFLYDAEATPTPGGGNTCVGAHCYR 534

Query: 546 VTFFVLSGVAGLGSILSIILTVRLRPVYQMLYA 572
           + F V++  + +G  L ++L  R + +Y  ++A
Sbjct: 547 LIFIVMALASVIGVGLDLVLAYRTKEIYAKIHA 534

BLAST of Lag0031267 vs. TAIR 10
Match: AT4G34950.1 (Major facilitator superfamily protein )

HSP 1 Score: 331.3 bits (848), Expect = 1.6e-90
Identity = 210/572 (36.71%), Postives = 321/572 (56.12%), Query Frame = 0

Query: 14  WVGLAAAVWVQIAAGSSYNFSLYSHLLKSVLGLNQQQLTVLGVANDIGESVGLLPGLACN 73
           W+G   AVWVQ  +G++Y FS YS  LKS++ L Q +L  L VA D+G++ G+L GLA +
Sbjct: 17  WLGFVTAVWVQSISGNNYTFSNYSGALKSLMNLTQLELNSLSVAKDVGKAFGILAGLASD 76

Query: 74  RFPPWAVLLVGSLCCFVGYGAIWLAVSRTVPNLPYWLLWLTHCIATNSNAWFGTAVLVTN 133
           R     +LL+GS    +GYG  WL VSRT+  +PYW + +  C+  NS  W  TAVLVT 
Sbjct: 77  RLSTPVILLIGSFEGLLGYGVQWLVVSRTIQPIPYWQMCVFLCMGGNSTTWMNTAVLVTC 136

Query: 134 MRNFPLSRGTVAGILKGYVGLSAAVYTVIYSMVLKKSALDLLLFLAIGIPILCLALMYFV 193
           +RNF  +RG V+GILKGYVGLS A++T + + +        L+ L++    +CL  ++F+
Sbjct: 137 IRNFRRNRGPVSGILKGYVGLSTAIFTDLCNALFSSDPASFLVLLSVVPFAVCLTAVFFL 196

Query: 194 RPCTPAS--SEDPSERSHFLFTQAACVLLAVFLVSTTIIDATTTPSDAVAYTLVAIMAIL 253
           R   P++  +ED  E  +F       V++AV+L S  II   T    A +    +I+ IL
Sbjct: 197 REIPPSTTFAEDNEESKYFAVFNIVAVVVAVYLQSYDIIGIKT---GAFSIAFASILLIL 256

Query: 254 LMSPLAVPIKMTICSA--KTKKLGPRVDSAEPLASGESDSSQIEPLLTPSSSATN----- 313
           L SP+AVP    I S     + +  R+D  EPL    S+    E ++  +++A N     
Sbjct: 257 LASPVAVPFHAFIRSKVHDEQDVEGRID--EPLLRSGSEIEVEETIVGAAAAADNELPPS 316

Query: 314 ---LGSFYENDDASDVETLLAVGEGAIHKKRRRPKRGEDFKLREAVIKADFWLLWFLYFL 373
              L +  E +  + V T           +++RP  GE+  + EA++  DFW+L+  +  
Sbjct: 317 LKPLSNEEEENHGTIVTT-----------EKKRPVLGEEHTIMEAMLTVDFWVLFVSFLC 376

Query: 374 GVGPGITVLNNLSQIGISLGVNDTTLLLALFSFCNFIGRLGSGVISEHFVSCSIYEMLIG 433
           GVG G+ V+NN+ QIG++LG  D ++ +++ S   F GR+ SG ISEHF+          
Sbjct: 377 GVGTGLAVMNNMGQIGLALGYTDVSIFVSMTSIWGFFGRILSGTISEHFI---------- 436

Query: 434 SAIVVQCRSRMIPRSLWMMFALILMSIAFLLYASALTSTLYLATGFTGISYGILYSMMVP 493
                  +    PR LW   A I+M++ +LL A AL  +LY+ +   G+ YG+  ++ VP
Sbjct: 437 -------KKAGTPRPLWNAAAQIIMAVGYLLMALALPGSLYIGSMVVGVCYGVRLAITVP 496

Query: 494 LASEIFGLKNFGVIFNFMQLGNPIGAVLFSVLLASTLYDNEAAKQ--GSITCIGQQCFRV 553
            ASE+FGLK +G+I+N + L  P+G+ LFS LLA  LYD EA     G  TC+G  CFR+
Sbjct: 497 TASELFGLKYYGLIYNILILNMPLGSFLFSGLLAGLLYDAEATPTPGGGNTCVGAHCFRI 555

Query: 554 TFFVLSGVAGLGSILSIILTVRLRPVYQMLYA 572
            F V++  + +G  L ++L  R + +Y  ++A
Sbjct: 557 VFIVMAFASIIGVGLDLLLAYRTKGIYAKIHA 555

BLAST of Lag0031267 vs. TAIR 10
Match: AT3G01930.2 (Major facilitator superfamily protein )

HSP 1 Score: 326.6 bits (836), Expect = 4.0e-89
Identity = 200/584 (34.25%), Postives = 312/584 (53.42%), Query Frame = 0

Query: 14  WVGLAAAVWVQIAAGSSYNFSLYSHLLKSVLGLNQQQLTVLGVANDIGESVGLLPGLACN 73
           W+   AA+W+Q  AG  Y F   S ++KS L  NQ+QL+ LGVA D+G+SVG L G    
Sbjct: 17  WLVFVAAMWIQSCAGIGYLFGSISPVIKSSLNYNQKQLSRLGVAKDLGDSVGFLAGTLSE 76

Query: 74  RFPPWAVLLVGSLCCFVGYGAIWLAVSRTVPNLPYWLLWLTHCIATNSNAWFGTAVLVTN 133
             P WA LLVGS+   VGYG +WL V+   P LP W + +   +  N   +F TA LV+ 
Sbjct: 77  ILPLWAALLVGSVQNLVGYGWVWLIVTGRAPILPLWAMCILIFVGNNGETYFNTAALVSG 136

Query: 134 MRNFPLSRGTVAGILKGYVGLSAAVYTVIYSMVLKKSALDLLLFLAIGIPILCLALMYFV 193
           ++NFP SRG V GILKG+ GL  A+ + +Y+M+       L+  +A+   ++ + LM+F+
Sbjct: 137 VQNFPKSRGPVVGILKGFAGLGGAILSQVYTMIHSSDRASLIFMVAVAPSVVVVPLMFFI 196

Query: 194 RPCTPASSEDPSERSHFLFTQAACVLLAVFLVSTTIIDATTTPSDAVAYTLVAIMAILLM 253
           RP         S+ + F    A C+LLA +L++  +++     S ++      ++  +L+
Sbjct: 197 RPVGGHRQIRSSDATSFTVIYAVCILLAAYLMAVMLVEDFIDLSHSIIIAFTVVLFAILL 256

Query: 254 SPLAVPIKMTICSAKTKKLGPRVDSAEPLASGESDSSQIEPLLTPSSSATNLGSFYENDD 313
            P+ +PI  +  +A T       D+ E    G+          TP      + S  E++ 
Sbjct: 257 VPIFIPIATSCFTASTDP----CDTLEEPLLGDQQGQDPGQSTTPDHGPELIFSEVEDEK 316

Query: 314 ASDVETLLAV-----------------GEGAIHKKRRR-PKRGEDFKLREAVIKADFWLL 373
             +V+ L AV                  EGA+  KRRR P RGEDF L +A++KADFWL+
Sbjct: 317 PKEVDLLPAVERHKRIAQLQAKLMQAAAEGAVRVKRRRGPHRGEDFTLTQALVKADFWLI 376

Query: 374 WFLYFLGVGPGITVLNNLSQIGISLGVNDTTLLLALFSFCNFIGRLGSGVISEHFVSCSI 433
           +F   LG G G+TV++NL Q+  SLG ++T + +++ S  NF+GR+G G  SE  V    
Sbjct: 377 FFSLLLGSGSGLTVIDNLGQMSQSLGYDNTHVFVSMISIWNFLGRIGGGYFSELIV---- 436

Query: 434 YEMLIGSAIVVQCRSRMIPRSLWMMFALILMSIAFLLYASALTSTLYLATGFTGISYGIL 493
                        R    PR + +  A ++MS+  + +A      +++ T   G+ YG  
Sbjct: 437 -------------RDYAYPRPVAIAVAQLVMSVGHIFFAYGWPGAMHIGTLLIGLGYGAH 496

Query: 494 YSMMVPLASEIFGLKNFGVIFNFMQLGNPIGAVLFSVLLASTLYDNEAAKQGS------- 553
           ++++   ASE+FGLK FG ++NF+ L NP G+++FS L+AS++YD EA +Q         
Sbjct: 497 WAIVPATASELFGLKKFGALYNFLTLANPAGSLVFSGLIASSIYDREAERQAQGSLFNPD 556

Query: 554 --ITCIGQQCFRVTFFVLSGVAGLGSILSIILTVRLRPVYQMLY 571
             + C G  C+ +T  ++SG   + + LS+IL  R +PVY  LY
Sbjct: 557 DVLRCRGSICYFLTSLIMSGFCLIAAALSMILVQRTKPVYTNLY 579

BLAST of Lag0031267 vs. TAIR 10
Match: AT5G14120.1 (Major facilitator superfamily protein )

HSP 1 Score: 325.5 bits (833), Expect = 8.9e-89
Identity = 205/584 (35.10%), Postives = 316/584 (54.11%), Query Frame = 0

Query: 14  WVGLAAAVWVQIAAGSSYNFSLYSHLLKSVLGLNQQQLTVLGVANDIGESVGLLPGLACN 73
           W+   AA+W+Q  AG  Y F   S ++KS L  NQ++L+ LGVA D+G+SVG + G    
Sbjct: 17  WLVFVAAMWIQSCAGIGYLFGSISPVIKSSLNYNQKELSRLGVAKDLGDSVGFIAGTLSE 76

Query: 74  RFPPWAVLLVGSLCCFVGYGAIWLAVSRTVPNLPYWLLWLTHCIATNSNAWFGTAVLVTN 133
             P WA LLVG++   +GYG +WL V+   P LP W + +   +  N   +F T  LV+ 
Sbjct: 77  ILPLWAALLVGAVQNLIGYGWVWLIVTGRAPILPLWAMCVLIFVGNNGETYFNTGALVSG 136

Query: 134 MRNFPLSRGTVAGILKGYVGLSAAVYTVIYSMVLKKSALDLLLFLAIGIPILCLALMYFV 193
           ++NFP SRG V GILKG+ GL  A+ + IY+M+   +   L+L +A+   ++ + LM+F+
Sbjct: 137 VQNFPKSRGPVVGILKGFAGLGGAIISQIYTMIHSSNPASLILMVAVTPAVVVVCLMFFI 196

Query: 194 RPCTPASSEDPSERSHFLFTQAACVLLAVFLVSTTIIDATTTPSDAVAYTLVAIMAILLM 253
           RP        P++ + F F    C+LLA +L+S  +I      S  V      ++ ++L+
Sbjct: 197 RPVGGHKQIRPTDGASFTFIYGVCLLLAAYLMSVMLIQDLVVVSHNVITVFTIVLFVILV 256

Query: 254 SPLAVPIKMTICSAKTKKLGPRVDSAEPLASGESDSSQIEPLLTPSSSATNLGSFYENDD 313
            P+ VPI MT    +T +    ++  EPL     D  Q   L TP    + +    E++ 
Sbjct: 257 VPILVPI-MTSFFTETNEPDDTIE--EPLVPKRED--QEPGLQTPDLILSEV----EDEK 316

Query: 314 ASDVETL-----------------LAVGEGAIHKKRRR-PKRGEDFKLREAVIKADFWLL 373
             DV+ L                  A  EGA+   RRR P RGEDF L +A++KADFWL+
Sbjct: 317 PKDVDLLPASERHKRIAHLQAQLMQAAAEGAVRVNRRRGPHRGEDFTLTQALVKADFWLI 376

Query: 374 WFLYFLGVGPGITVLNNLSQIGISLGVNDTTLLLALFSFCNFIGRLGSGVISEHFVSCSI 433
           +F   LG G G+TV++NL Q+  SLG ++T +L+++ S  NF+GR+G G  SE  V    
Sbjct: 377 FFSLLLGSGSGLTVIDNLGQMSQSLGYDNTHVLVSMISIWNFLGRIGGGYFSELVV---- 436

Query: 434 YEMLIGSAIVVQCRSRMIPRSLWMMFALILMSIAFLLYASALTSTLYLATGFTGISYGIL 493
                        R    PR + M  A ++MS+  + +A      +Y+ T   G+ YG  
Sbjct: 437 -------------RDYAYPRPVAMAVAQLIMSVGHIFFAYGWPGAMYIGTLLIGLGYGAH 496

Query: 494 YSMMVPLASEIFGLKNFGVIFNFMQLGNPIGAVLFSVLLASTLYDNEAAKQG-------- 553
           ++++   ASE+FGLK FG ++NF+ L NP G+++FS ++AS++YD EA +Q         
Sbjct: 497 WAIVPATASELFGLKKFGALYNFLTLANPAGSLVFSGMIASSIYDREAERQAHGSVFDPD 556

Query: 554 -SITCIGQQCFRVTFFVLSGVAGLGSILSIILTVRLRPVYQMLY 571
            ++ C G  CF +T  ++SG   +  +LS+IL  R + VY  LY
Sbjct: 557 DALRCNGSICFFLTSLIMSGFCIIACMLSMILVRRTKSVYTHLY 574

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7018369.12.1e-29791.94Protein NUCLEAR FUSION DEFECTIVE 4 [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_023528220.13.5e-29791.94protein NUCLEAR FUSION DEFECTIVE 4 [Cucurbita pepo subsp. pepo][more]
XP_022956065.14.6e-29791.77protein NUCLEAR FUSION DEFECTIVE 4 [Cucurbita moschata][more]
XP_022979616.16.6e-29691.42protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita maxima][more]
XP_038896285.11.9e-29591.60protein NUCLEAR FUSION DEFECTIVE 4 isoform X1 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
F4I9E13.4e-4528.55Protein NUCLEAR FUSION DEFECTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=NFD4 PE=3 ... [more]
Q4IM486.6e-0424.77Probable transporter MCH1 OS=Gibberella zeae (strain ATCC MYA-4620 / CBS 123657 ... [more]
Match NameE-valueIdentityDescription
A0A6J1GWT32.2e-29791.77protein NUCLEAR FUSION DEFECTIVE 4 OS=Cucurbita moschata OX=3662 GN=LOC111457872... [more]
A0A6J1IRA63.2e-29691.42protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita maxima OX=3661 GN=LOC111479... [more]
A0A0A0KTP32.5e-29389.88Nodulin-like domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G00630... [more]
A0A5D3E0Y72.2e-29289.88Protein NUCLEAR FUSION DEFECTIVE 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A1S3BY606.3e-29289.88LOW QUALITY PROTEIN: protein NUCLEAR FUSION DEFECTIVE 4 OS=Cucumis melo OX=3656 ... [more]
Match NameE-valueIdentityDescription
AT1G80530.11.2e-18658.02Major facilitator superfamily protein [more]
AT2G16660.17.2e-9136.47Major facilitator superfamily protein [more]
AT4G34950.11.6e-9036.71Major facilitator superfamily protein [more]
AT3G01930.24.0e-8934.25Major facilitator superfamily protein [more]
AT5G14120.18.9e-8935.10Major facilitator superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036259MFS transporter superfamilyGENE3D1.20.1250.20MFS general substrate transporter like domainscoord: 347..566
e-value: 7.7E-7
score: 30.7
IPR036259MFS transporter superfamilyGENE3D1.20.1250.20MFS general substrate transporter like domainscoord: 8..276
e-value: 5.1E-7
score: 30.7
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 333..564
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 13..199
IPR010658Nodulin-likePFAMPF06813Nodulin-likecoord: 13..260
e-value: 1.3E-91
score: 306.5
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 276..297
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 283..297
NoneNo IPR availablePANTHERPTHR21576:SF97MAJOR FACILITATOR SUPERFAMILY PROTEINcoord: 3..583
NoneNo IPR availablePANTHERPTHR21576UNCHARACTERIZED NODULIN-LIKE PROTEINcoord: 3..583
NoneNo IPR availableCDDcd17354MFS_Mch1p_likecoord: 12..551
e-value: 2.87962E-103
score: 315.345

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0031267.1Lag0031267.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0022857 transmembrane transporter activity