Lag0031241 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0031241
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
Descriptionmetal transporter Nramp6-like
Locationchr11: 6133473 .. 6139116 (+)
RNA-Seq ExpressionLag0031241
SyntenyLag0031241
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTGCTGGAGGGTCTAATTCTGGGCAGCCGCAGTTCCTTGCTAGGGCTGGAGATGAGAGTTTTTCGCACGCACCCTTGATTGAGAACCCGGAAACTGATCAGATTGTCGTTCCTGACGTGAGCCTTAATTTTTGTTGTTCTGATGATTAATCGTTGAGAATTTACGAATTGTTGGTTAATTAGTCATTTAATTGTACTTTGAATAAGAGGACGTTAAAGTTTAAGCAACTTGTAAGCTATGAAGTATTTAGCTATGGACAAAAGTGCAAAGGAATTTTTTGATTGAGAGCTACTGTTGCGTGTTATGTGCAGAAGAAAAGCTGGAAGAACCTTTTTGCATACATGGGCCCGGGGTTTCTTGTTTCTATTGCCTATATTGATCCTGGGAATTGTAAGATCTTTTATACACTACTTTTAGATTTGTGGTTTGTGTCTTTTGTTCCATTATGAATGGGATTCTGAAGATTTTGTATGGTAATTCGCAGTTGAAACTGATCTACAATCTGGAGCACAGTACAAGTATGGGGTACGATATCGTTGCCTTACTTTTAACAACCAACAAACACATCATTTTGTGGTCAAAGGCCACAATTTGTTATCATCTTTCAATTTTTTTTATTCCACATCCTTCTATATGGATGGATCAGTTAATTGAGTAGTAAACTAAGTATTCTGCTGTGAAGAGCTGAGATGATCCATTCACCATTTACCATAGGCTTTTTCATTTGTATCATTTCTGTTCAACTATAAAAATTATCATGCTATAGATTATAAAAACATATGTAAAGTTCTGCCTCCAGCCTAATCCCATAAACTTACTCAAGACAAATTTCAGAACTAGATTTTCTCTAAGTTTTCAATTAGTACTGAACATTCTGAATAGGTTCCAATTTTTGTTATACTTTTTCTTGTCGAAGCTAGAAAGACCAAAGTCCTGTCTTGTCATTCAAATTTCACATGCTTGTTATATTCCTAATGGACCTGACGACTAACACCCCTTCTCCCGGCACAGTTGCTTTGGATCATATTAGTGGCTTCGTGTGCTGCCCTTATCATTCAATCCTTGGCAGCAAATCTAGGGGTTGTCACAGGTAATGACTTCTGCATAACTTTTTCTTTTTCTCTAAATTGTGATATTTCTATAGTTCCTAGAGTTTTTAGACATTTTTCTCTTGTTTTTTTGGTAAGTGTAGGAAAACATTTAGCAGAGCACTGTAAGTCTGAGTACCCCAAGGTGCAAAATTTCATCCTATGGATCCTTGCTGAAATTGCAATTGTTGCATGTGATATTCCCGAAGGTAAAGTAATCCTATTCATTAGAAACCTAATAGTTGAGTGAAAAATACTCTTGGTCATAGAAGAAATAAGTAGAATTGTTGCATGTTTAGTCTTTTGCATTTTCTGAGATTTGTACAACAGCAGTTACCCAATTGAAAAGTTTTTGGCCCTCAGTGGGTGGACCACCCAAACAATGCATTTCAAAATAGCTCCCTACTTGTTGTTGGGCGATTGTTTGAGTGAGTCCTATCGGCTTGACCTCTTTTCCTCGGTAAATCATCACTCTTTGTCGTCTGTCAAAAAAATCCATATACAACCAGGATTGGTCAAAGTGCTTCTTGAAACAAGCACAAAATAAGGGTTAGGAACTAACTTGTATTAGGAGAGGAGTAGGATATGTTTCAACCTATAATAGGAGTCCTTAGACCTTGTTGTTTTGGCATACAAGTAAAGACGAACATGAATAGGAGCAGGAGCAGGCAATGGAATTGTATCATAACATGAATTTGTTGTGATCATCTCTATTTTAGGTATTCAGGGAATAAGCAGTTACCCTATCCAATTGAAAAGTTTTTGAGTGGAGATTTAATTAGTTGCCGATACGAATGGGTTGTTAAGTCTAGTATTGCTAGCATCAGCGGGTGAAGGAGAAGGGAAATTTCCTAACTACTCAGCATATGTTGTAAAAGCTGATTAGATGTCCATCAACCAACTGTTGTTGTTTGACATTGATATGATCAAGTGTACAAGCCAAACCGAAAAGCACATATCAAGTTAACAATATATAAACACAGGATCATGGCTTTCATGAGGAGAAGAAAATTATCATCATTATTATTTTCTTGTGGAAGACCAGTGTTTAGAGTTGCTATGGGGGTGTTGTATTCTGTATCTGCTTCATGACATTCATTGGTATAAAATTGTTGTTTCTTGTTGAAGTTATTCTTATATCTCCTCCTTTCTGTTTCCAGTTATAGGAACCGCTTTTGCATTGAATATGCTCTTCAGCATTCCTGTTTGGTGTGGCGTTCTTTTGACAGGGCTTAGTACACTGTTCCTTCTTGCGCTGCAGCAATATGGGGTATTTTGAGATTTACTTTATTCTGGATTTTACTTATTTTGCAGTAACCAAGATTCTAAGCCTTGCATCAATATGATTGGGAAATAATTCTCCCATTTGTGTTATTACAGCGAAGGAGGTAGCATAAGTTTAGACAACTAAACTTTACCTACTTTAAATGCTCACCTCGTTTATGTTTATATCCACTATGCAGATTAGAAAGCTCGAATTCTTGATTGCGTTTCTTGTATTGACAATTGCAATTTGCTTCTTCCTGGAGCTGGGCTATGCAAAGCCCGACGTCGGAGAAATTTTTTATGGGTTATTTGTCCCTCAATTAAAAGGAAGTGGTGCTACTGGTCTTGCAATTTCACTTCTTGGTGCTATGGTTATGCCGTATGTCTGTTATATCCTTCAATAACATTCACAGCCTTCATTTTTTTTATCCCTTACACTTCAATATTCTAAATTTAACTACCTGCTGCAGACACAATCTCTTTCTTCACTCGGCACTGGTGCTCTCTAGGAAAATACCCCGATCTGTTTCTGGCATCAAGGTGATGTTTCGTCTTCATCAGTCTAATCTGCAAATTCCTTTATACTTAATACTTTCCAAATGTGTTTAAAATTTATTCAAATTTTCATTTAATATCTAAGTATCTCATAGTTTCATAGAAGCATGCTTCAAAGCTTTGGTTGCTACTTTATTATTTAAATTGTAATTCTACATTTTACTGTACTAACACTCACACATTTTCTTCACAGGAAGCTTGCAGATTTTATCTGATAGAGAGTGGCTTTGCTCTTATGGTGGCTTTCTTCATTAATGTGTCAGTCATTTCTGTTAGTGGTGCAGTTTGCAATTCCCCAGATTTAAATAAGGAGGACCAAATGAGCTGCAATGACTTGGACTTGAATAAAGCTTCATTTTTATTGAGAGTATGTGTGATCTAGCAACTTTTGCCTATATCCTTCTTGTTTACCTCATGGGATTTGGTTCCTCGCCAATGGTGCGCTAGTTTCATACAGAGGTCTTCTATACTTTTTTCAGAATGTATTGGGTAAATGGAGTTCAAAACTTTTTGCCATTGCTTTGTTAGCATCTGGTCAGAGTTCTACGATAACAGGAACCTATGCAGGACAGTATGTTATGCAGGTACTATGCTGGTCCTCTTTCTATGTTGAAGCTCTGCTTCTTTACATTTCGAATCAATGCTCTCATCCGTTTATATTATTGGTATTGAGTAAATCAGAAATAGCATGGTTATGAAATTTTCTGAATGTTTATGGTCATAAACAGGACCTACTTGTGGGTACTCCATAATACTTTGATTTCAAGTAGTTTCTTTGAATTTTTTTCCTTTTTGACACATTCCTGTTTATACCACATTTCTTTTGTAGGGGTTTCTTGATTTGAAACTGACGCCATGGATCAGAAACTTTTTAACCCGAAGCTTAGCAATTGTTCCTAGTTTGATTGTTGCAATCATTGGTGGGTCTTCTGGGGCTGGGCAGTTGATTATTATTGCGTCGGTAAGGATATATACTTCCCCATGAATGTGATTGAGGTTGAACTGCCCATCAGTATATGCATGGCCAATACCTCCGTCTTTGTTCCATGCATGTTATTTAACGAAGTCAATCGAGGTAGAAGTTGATAGAAAATGATAGTGAAATTCACTTTGAAACTTGTATCAGCGTTGATCGAAGTGGTATACCGATCAACAACCCTAGTCATTGAATAGGTTTCTTATTTCATATGCTTAGGATTTAATGCTGTTCCACCATCTTTTTCACAGATTTCACTAAGGGCCCAAGTTCTTTGGCATAGTTCAATTGTAGTGTGCTGATCAATTACTGCTTTAAATTGAAGGCAAAGCGTTTTGACTCCCTGCAATCTTCTCTTTTGGCAGATGATCTTGTCATTTGAACTCCCTTTTGCTCTCATTCCCCTTCTGAAGTTTACAAGCAGCAAGACCAAGATGGGACCGCATGTCAACTCCACCGCGGTAATTTCGACTGATCTAAGTAGCTTCTCTATAACATTTGCTCTTTCCTTTTGTTAGATGATCCCAAACAAAGCTTGAGCTAAACATCCTTCAAATCCATACAACAAAAGAATTTGGATTAAAAATGGTACTCCCTAACATCCACCAACATCATGAAGAAAATAACACTTAATTATTTGCTGAAGTAGTTCTTGTCTTGAAGGGTGCACATTAGAGTTGAATTTGAAGCCTTTTATGAATAGTTTGTAATTAATCTTCAAATAAGCATTCATAACATTTTTTATCATACTAATCCTTATAACCAAAGAAAGGCTGGATTATTCTGCTTGAAATGCATTCTCCTTTTAGTTTTATCTTCAAGGATGAACTGATGAATGACATGAAATGGGTCTCACACTCCTGACAAAACCAAGCTGATCATCATAGTTTTCTGATCATTTTGATCAATTTTCATTATATATTTTCGAAATGCAGATTACGGTACTGACATGGATCATCGGTTTCCTCATCATGGCTATAAATATATACTACCTCATAACCCGATTCATCCACGTGCTTCTTCATAACGATCTCCAACTTGTAGCAGTCGTCTTCATAGGGATACTCGGATTTTCAGGCATGGCATTATATTTAGCTGGAATCGCCTATCTAGTTCTCAGAAAGACCAAGGAGATCACTCATTTCTTAGCACTAACAACAGAAGAAAGTCAACGACTGAGTAACGAGCCGAGTAAGACATCGGGATATAATCTCCCGAGCGAAGATATAGTAAGCATGCAATTGCCTCAAAGAATTAGAACTACCAATGATGTAAACTGACAGATAATAGTTAAAAACTTCAGCCATAGAAGGAAGGCAGGATCCTTTGGGTGCTGGTTTCATTTGTTTTGGAAATAGAACAGACAGATGAAACTCAGAAGCACAGCAAAATGGAAAGGAAAAAAAGTTTAATCAGCTTAAAGCCAATTCTCTACTTGTATCATCAATCATCATCATCATCACCTTCAATACTGCCTAGCTAGCTAAGCTAACTGCATGACTTTTGCAGTGTTCACTTGAGCTTTTTAGGGTTCTTAGCACAGCAATAATTAAATACTTACCTTTTTGCCCATCTTTTTTTACCCTCTCTTCTCTTAGAAATGACACTTTATTACTATTGTTTTTGGGTTTTAACATTTAATCCAACATTAGCTGATAAACTGTTGATTGGTGATTTGATATGGTATCAAAACAGGAGATCATGTGTCCAGGGATTCTTGCTGGAATCAGTCAACAGGATTATGCGTCCGTATAG

mRNA sequence

ATGGCTGCTGGAGGGTCTAATTCTGGGCAGCCGCAGTTCCTTGCTAGGGCTGGAGATGAGAGTTTTTCGCACGCACCCTTGATTGAGAACCCGGAAACTGATCAGATTGTCGTTCCTGACAAGAAAAGCTGGAAGAACCTTTTTGCATACATGGGCCCGGGGTTTCTTGTTTCTATTGCCTATATTGATCCTGGGAATTTTGAAACTGATCTACAATCTGGAGCACAGTACAAGTATGGGTTGCTTTGGATCATATTAGTGGCTTCGTGTGCTGCCCTTATCATTCAATCCTTGGCAGCAAATCTAGGGGTTGTCACAGGAAAACATTTAGCAGAGCACTGTAAGTCTGAGTACCCCAAGGTGCAAAATTTCATCCTATGGATCCTTGCTGAAATTGCAATTGTTGCATGTGATATTCCCGAAGTTATAGGAACCGCTTTTGCATTGAATATGCTCTTCAGCATTCCTGTTTGGTGTGGCGTTCTTTTGACAGGGCTTAGTACACTGTTCCTTCTTGCGCTGCAGCAATATGGGATTAGAAAGCTCGAATTCTTGATTGCGTTTCTTGTATTGACAATTGCAATTTGCTTCTTCCTGGAGCTGGGCTATGCAAAGCCCGACGTCGGAGAAATTTTTTATGGGTTATTTGTCCCTCAATTAAAAGGAAGTGGTGCTACTGGTCTTGCAATTTCACTTCTTGGTGCTATGGTTATGCCACACAATCTCTTTCTTCACTCGGCACTGGTGCTCTCTAGGAAAATACCCCGATCTGTTTCTGGCATCAAGGAAGCTTGCAGATTTTATCTGATAGAGAGTGGCTTTGCTCTTATGGTGGCTTTCTTCATTAATGTGTCAGTCATTTCTGTTAGTGGTGCAGTTTGCAATTCCCCAGATTTAAATAAGGAGGACCAAATGAGCTGCAATGACTTGGACTTGAATAAAGCTTCATTTTTATTGAGAAATGTATTGGGTAAATGGAGTTCAAAACTTTTTGCCATTGCTTTGTTAGCATCTGGTCAGAGTTCTACGATAACAGGAACCTATGCAGGACAGTATGTTATGCAGGGGTTTCTTGATTTGAAACTGACGCCATGGATCAGAAACTTTTTAACCCGAAGCTTAGCAATTGTTCCTAGTTTGATTGTTGCAATCATTGGTGGGTCTTCTGGGGCTGGGCAGTTGATTATTATTGCGTCGATGATCTTGTCATTTGAACTCCCTTTTGCTCTCATTCCCCTTCTGAAGTTTACAAGCAGCAAGACCAAGATGGGACCGCATGTCAACTCCACCGCGATTACGGTACTGACATGGATCATCGGTTTCCTCATCATGGCTATAAATATATACTACCTCATAACCCGATTCATCCACGTGCTTCTTCATAACGATCTCCAACTTGTAGCAGTCGTCTTCATAGGGATACTCGGATTTTCAGGCATGGCATTATATTTAGCTGGAATCGCCTATCTAGTTCTCAGAAAGACCAAGGAGATCACTCATTTCTTAGCACTAACAACAGAAGAAAGTCAACGACTGAGTAACGAGCCGAGTAAGACATCGGGATATAATCTCCCGAGCGAAGATATAGAGATCATGTGTCCAGGGATTCTTGCTGGAATCAGTCAACAGGATTATGCGTCCGTATAG

Coding sequence (CDS)

ATGGCTGCTGGAGGGTCTAATTCTGGGCAGCCGCAGTTCCTTGCTAGGGCTGGAGATGAGAGTTTTTCGCACGCACCCTTGATTGAGAACCCGGAAACTGATCAGATTGTCGTTCCTGACAAGAAAAGCTGGAAGAACCTTTTTGCATACATGGGCCCGGGGTTTCTTGTTTCTATTGCCTATATTGATCCTGGGAATTTTGAAACTGATCTACAATCTGGAGCACAGTACAAGTATGGGTTGCTTTGGATCATATTAGTGGCTTCGTGTGCTGCCCTTATCATTCAATCCTTGGCAGCAAATCTAGGGGTTGTCACAGGAAAACATTTAGCAGAGCACTGTAAGTCTGAGTACCCCAAGGTGCAAAATTTCATCCTATGGATCCTTGCTGAAATTGCAATTGTTGCATGTGATATTCCCGAAGTTATAGGAACCGCTTTTGCATTGAATATGCTCTTCAGCATTCCTGTTTGGTGTGGCGTTCTTTTGACAGGGCTTAGTACACTGTTCCTTCTTGCGCTGCAGCAATATGGGATTAGAAAGCTCGAATTCTTGATTGCGTTTCTTGTATTGACAATTGCAATTTGCTTCTTCCTGGAGCTGGGCTATGCAAAGCCCGACGTCGGAGAAATTTTTTATGGGTTATTTGTCCCTCAATTAAAAGGAAGTGGTGCTACTGGTCTTGCAATTTCACTTCTTGGTGCTATGGTTATGCCACACAATCTCTTTCTTCACTCGGCACTGGTGCTCTCTAGGAAAATACCCCGATCTGTTTCTGGCATCAAGGAAGCTTGCAGATTTTATCTGATAGAGAGTGGCTTTGCTCTTATGGTGGCTTTCTTCATTAATGTGTCAGTCATTTCTGTTAGTGGTGCAGTTTGCAATTCCCCAGATTTAAATAAGGAGGACCAAATGAGCTGCAATGACTTGGACTTGAATAAAGCTTCATTTTTATTGAGAAATGTATTGGGTAAATGGAGTTCAAAACTTTTTGCCATTGCTTTGTTAGCATCTGGTCAGAGTTCTACGATAACAGGAACCTATGCAGGACAGTATGTTATGCAGGGGTTTCTTGATTTGAAACTGACGCCATGGATCAGAAACTTTTTAACCCGAAGCTTAGCAATTGTTCCTAGTTTGATTGTTGCAATCATTGGTGGGTCTTCTGGGGCTGGGCAGTTGATTATTATTGCGTCGATGATCTTGTCATTTGAACTCCCTTTTGCTCTCATTCCCCTTCTGAAGTTTACAAGCAGCAAGACCAAGATGGGACCGCATGTCAACTCCACCGCGATTACGGTACTGACATGGATCATCGGTTTCCTCATCATGGCTATAAATATATACTACCTCATAACCCGATTCATCCACGTGCTTCTTCATAACGATCTCCAACTTGTAGCAGTCGTCTTCATAGGGATACTCGGATTTTCAGGCATGGCATTATATTTAGCTGGAATCGCCTATCTAGTTCTCAGAAAGACCAAGGAGATCACTCATTTCTTAGCACTAACAACAGAAGAAAGTCAACGACTGAGTAACGAGCCGAGTAAGACATCGGGATATAATCTCCCGAGCGAAGATATAGAGATCATGTGTCCAGGGATTCTTGCTGGAATCAGTCAACAGGATTATGCGTCCGTATAG

Protein sequence

MAAGGSNSGQPQFLARAGDESFSHAPLIENPETDQIVVPDKKSWKNLFAYMGPGFLVSIAYIDPGNFETDLQSGAQYKYGLLWIILVASCAALIIQSLAANLGVVTGKHLAEHCKSEYPKVQNFILWILAEIAIVACDIPEVIGTAFALNMLFSIPVWCGVLLTGLSTLFLLALQQYGIRKLEFLIAFLVLTIAICFFLELGYAKPDVGEIFYGLFVPQLKGSGATGLAISLLGAMVMPHNLFLHSALVLSRKIPRSVSGIKEACRFYLIESGFALMVAFFINVSVISVSGAVCNSPDLNKEDQMSCNDLDLNKASFLLRNVLGKWSSKLFAIALLASGQSSTITGTYAGQYVMQGFLDLKLTPWIRNFLTRSLAIVPSLIVAIIGGSSGAGQLIIIASMILSFELPFALIPLLKFTSSKTKMGPHVNSTAITVLTWIIGFLIMAINIYYLITRFIHVLLHNDLQLVAVVFIGILGFSGMALYLAGIAYLVLRKTKEITHFLALTTEESQRLSNEPSKTSGYNLPSEDIEIMCPGILAGISQQDYASV
Homology
BLAST of Lag0031241 vs. NCBI nr
Match: XP_038905496.1 (metal transporter Nramp6-like [Benincasa hispida] >XP_038905497.1 metal transporter Nramp6-like [Benincasa hispida])

HSP 1 Score: 975.7 bits (2521), Expect = 1.6e-280
Identity = 508/532 (95.49%), Postives = 519/532 (97.56%), Query Frame = 0

Query: 1   MAAGGSNSGQPQFLARAGDESFSHAPLIENPETDQIVVPDKKSWKNLFAYMGPGFLVSIA 60
           MAAGGS SGQPQFL RAGDESFSHAPLIENPETDQI+VPDKKSWKNLFAYMGPGFLVSIA
Sbjct: 1   MAAGGSGSGQPQFLVRAGDESFSHAPLIENPETDQIIVPDKKSWKNLFAYMGPGFLVSIA 60

Query: 61  YIDPGNFETDLQSGAQYKYGLLWIILVASCAALIIQSLAANLGVVTGKHLAEHCKSEYPK 120
           YIDPGNFETDLQSGAQYKYGLLWIILVAS AALIIQSLAANLGVVTGKHLAEHCK+EYPK
Sbjct: 61  YIDPGNFETDLQSGAQYKYGLLWIILVASFAALIIQSLAANLGVVTGKHLAEHCKAEYPK 120

Query: 121 VQNFILWILAEIAIVACDIPEVIGTAFALNMLFSIPVWCGVLLTGLSTLFLLALQQYGIR 180
            QNFILW+LAEIAIVACDIPEVIGTAFALNMLFSIPVWCGVLLTGLSTL LLALQQYGIR
Sbjct: 121 AQNFILWVLAEIAIVACDIPEVIGTAFALNMLFSIPVWCGVLLTGLSTLLLLALQQYGIR 180

Query: 181 KLEFLIAFLVLTIAICFFLELGYAKPDVGEIFYGLFVPQLKGSGATGLAISLLGAMVMPH 240
           KLEFLIAFLVLTIAICFFLELGYAKPDVGEI YGLFVPQLKGSGATGLAISLLGAMVMPH
Sbjct: 181 KLEFLIAFLVLTIAICFFLELGYAKPDVGEILYGLFVPQLKGSGATGLAISLLGAMVMPH 240

Query: 241 NLFLHSALVLSRKIPRSVSGIKEACRFYLIESGFALMVAFFINVSVISVSGAVCNSPDLN 300
           NLFLHSALVLSRKIPRS+SGIKEACRFY+IESGFALMVAF INVSVISVSGAVCNSPDLN
Sbjct: 241 NLFLHSALVLSRKIPRSLSGIKEACRFYMIESGFALMVAFLINVSVISVSGAVCNSPDLN 300

Query: 301 KEDQMSCNDLDLNKASFLLRNVLGKWSSKLFAIALLASGQSSTITGTYAGQYVMQGFLDL 360
           KEDQMSCNDLDLNKASFLLRNVLGKWSSKLFAIALLASGQSSTITGTYAGQYVMQGFLDL
Sbjct: 301 KEDQMSCNDLDLNKASFLLRNVLGKWSSKLFAIALLASGQSSTITGTYAGQYVMQGFLDL 360

Query: 361 KLTPWIRNFLTRSLAIVPSLIVAIIGGSSGAGQLIIIASMILSFELPFALIPLLKFTSSK 420
           KLTPWIRNFLTRSLAIVPSLIVAIIGGSSGAG+LIIIASMILSFELPFAL+PLLKFTSSK
Sbjct: 361 KLTPWIRNFLTRSLAIVPSLIVAIIGGSSGAGKLIIIASMILSFELPFALVPLLKFTSSK 420

Query: 421 TKMGPHVNSTAITVLTWIIGFLIMAINIYYLITRFIHVLLHNDLQLVAVVFIGILGFSGM 480
           TKMGPHVNSTAITVLTWIIGFLIMAINIYYL+TRFIHV+LHNDLQL  VVFIGILGFSGM
Sbjct: 421 TKMGPHVNSTAITVLTWIIGFLIMAINIYYLMTRFIHVILHNDLQLAVVVFIGILGFSGM 480

Query: 481 ALYLAGIAYLVLRKTKEITHFLALTTEESQRLSNEPSKTSGYNLPSEDIEIM 533
           ALYLAGIAYLVLRKTKEITH LALTTEES+RLSNEPSKTSGY LP+EDI  M
Sbjct: 481 ALYLAGIAYLVLRKTKEITHLLALTTEESRRLSNEPSKTSGYGLPNEDIVSM 532

BLAST of Lag0031241 vs. NCBI nr
Match: XP_022982780.1 (metal transporter Nramp6-like [Cucurbita maxima])

HSP 1 Score: 970.3 bits (2507), Expect = 6.9e-279
Identity = 503/529 (95.09%), Postives = 519/529 (98.11%), Query Frame = 0

Query: 1   MAAGGSNSGQPQFLARAGDESFSHAPLIENPETDQIVVPDKKSWKNLFAYMGPGFLVSIA 60
           MAAGGS+SGQPQF+ARAG+ESFSHAPLIENPET QIVVPDKKSWKNLFAYMGPGFLVSIA
Sbjct: 1   MAAGGSSSGQPQFIARAGNESFSHAPLIENPETGQIVVPDKKSWKNLFAYMGPGFLVSIA 60

Query: 61  YIDPGNFETDLQSGAQYKYGLLWIILVASCAALIIQSLAANLGVVTGKHLAEHCKSEYPK 120
           YIDPGNFETDLQSGAQYKYGLLWIILVASCAALIIQSLAANLGVVTGKHLAEHCK+EYPK
Sbjct: 61  YIDPGNFETDLQSGAQYKYGLLWIILVASCAALIIQSLAANLGVVTGKHLAEHCKAEYPK 120

Query: 121 VQNFILWILAEIAIVACDIPEVIGTAFALNMLFSIPVWCGVLLTGLSTLFLLALQQYGIR 180
           VQNFILW+LAEIAIVACDIPEVIGTAFALNMLFSIPVWCGVLLTGLSTLFLLALQQYGIR
Sbjct: 121 VQNFILWVLAEIAIVACDIPEVIGTAFALNMLFSIPVWCGVLLTGLSTLFLLALQQYGIR 180

Query: 181 KLEFLIAFLVLTIAICFFLELGYAKPDVGEIFYGLFVPQLKGSGATGLAISLLGAMVMPH 240
           KLEFLIAFLVLTIAICFFLELGYAKPDVGE+FYGLFVPQLKGSGATGLAISLLGAMVMPH
Sbjct: 181 KLEFLIAFLVLTIAICFFLELGYAKPDVGEVFYGLFVPQLKGSGATGLAISLLGAMVMPH 240

Query: 241 NLFLHSALVLSRKIPRSVSGIKEACRFYLIESGFALMVAFFINVSVISVSGAVCNSPDLN 300
           NLFLHSALVLSRKIPRSVSGIKEACRFY+IESGFALMVAF INVSVISVSGAVCNSPDLN
Sbjct: 241 NLFLHSALVLSRKIPRSVSGIKEACRFYMIESGFALMVAFLINVSVISVSGAVCNSPDLN 300

Query: 301 KEDQMSCNDLDLNKASFLLRNVLGKWSSKLFAIALLASGQSSTITGTYAGQYVMQGFLDL 360
           KEDQMSCNDLDLNKASFLLRNVLGKWSSKLFAIALLASGQSSTITGTYAGQYVMQGFLDL
Sbjct: 301 KEDQMSCNDLDLNKASFLLRNVLGKWSSKLFAIALLASGQSSTITGTYAGQYVMQGFLDL 360

Query: 361 KLTPWIRNFLTRSLAIVPSLIVAIIGGSSGAGQLIIIASMILSFELPFALIPLLKFTSSK 420
           KLTPW+RNFLTR LAIVPSLIVAIIGGSSGAG+LIIIASMILSFELPFAL+PLLKFTSS+
Sbjct: 361 KLTPWVRNFLTRCLAIVPSLIVAIIGGSSGAGKLIIIASMILSFELPFALVPLLKFTSSR 420

Query: 421 TKMGPHVNSTAITVLTWIIGFLIMAINIYYLITRFIHVLLHNDLQLVAVVFIGILGFSGM 480
           TKMG HVNSTAITVLTWIIGFLIMAINIYYL+TRFIHVLLHNDLQLVAVVFIGILGFSGM
Sbjct: 421 TKMGTHVNSTAITVLTWIIGFLIMAINIYYLMTRFIHVLLHNDLQLVAVVFIGILGFSGM 480

Query: 481 ALYLAGIAYLVLRKTKEITHFLALTTEESQRLSNEPSKTSGYNLPSEDI 530
           A+YLAGIAYLV RKTKE TH LALTTEESQ+LSN PSK SGY LP+ED+
Sbjct: 481 AIYLAGIAYLVFRKTKESTHLLALTTEESQQLSNTPSKRSGYGLPNEDL 529

BLAST of Lag0031241 vs. NCBI nr
Match: KAG6581627.1 (Metal transporter Nramp1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 968.0 bits (2501), Expect = 3.4e-278
Identity = 503/532 (94.55%), Postives = 519/532 (97.56%), Query Frame = 0

Query: 1   MAAGGSNSGQPQFLARAGDESFSHAPLIENPETDQIVVPDKKSWKNLFAYMGPGFLVSIA 60
           MAAGGS+SGQPQF+ARAG+ESFSHAPLIENPETDQIVVPDKKSWKNLFAYMGPGFLVSIA
Sbjct: 1   MAAGGSSSGQPQFIARAGNESFSHAPLIENPETDQIVVPDKKSWKNLFAYMGPGFLVSIA 60

Query: 61  YIDPGNFETDLQSGAQYKYGLLWIILVASCAALIIQSLAANLGVVTGKHLAEHCKSEYPK 120
           YIDPGNFETDLQSGAQYKY LLWIILVASCAALIIQSLAANLGVVTGKHLAEHCK+EYPK
Sbjct: 61  YIDPGNFETDLQSGAQYKYELLWIILVASCAALIIQSLAANLGVVTGKHLAEHCKAEYPK 120

Query: 121 VQNFILWILAEIAIVACDIPEVIGTAFALNMLFSIPVWCGVLLTGLSTLFLLALQQYGIR 180
           VQNFILW+LAEIAIVACDIPEVIGTAFALNMLFSIPVWCGVLLTGLSTLFLLALQQYGIR
Sbjct: 121 VQNFILWVLAEIAIVACDIPEVIGTAFALNMLFSIPVWCGVLLTGLSTLFLLALQQYGIR 180

Query: 181 KLEFLIAFLVLTIAICFFLELGYAKPDVGEIFYGLFVPQLKGSGATGLAISLLGAMVMPH 240
           KLEFLIAFLVLTIAICFFLELGYAKPDVGE+FYGLFVPQLKGSGATGLAISLLGAMVMPH
Sbjct: 181 KLEFLIAFLVLTIAICFFLELGYAKPDVGEVFYGLFVPQLKGSGATGLAISLLGAMVMPH 240

Query: 241 NLFLHSALVLSRKIPRSVSGIKEACRFYLIESGFALMVAFFINVSVISVSGAVCNSPDLN 300
           NLFLHSALVLSRKIPRSVSGIKEACRFY+IESGFALMVAF INVSVISVSGAVCNSPDLN
Sbjct: 241 NLFLHSALVLSRKIPRSVSGIKEACRFYMIESGFALMVAFLINVSVISVSGAVCNSPDLN 300

Query: 301 KEDQMSCNDLDLNKASFLLRNVLGKWSSKLFAIALLASGQSSTITGTYAGQYVMQGFLDL 360
           KEDQMSCNDLDLNKASFLLRNVLGKWSSKLFAIALLASGQSSTITGTYAGQYVMQGFLDL
Sbjct: 301 KEDQMSCNDLDLNKASFLLRNVLGKWSSKLFAIALLASGQSSTITGTYAGQYVMQGFLDL 360

Query: 361 KLTPWIRNFLTRSLAIVPSLIVAIIGGSSGAGQLIIIASMILSFELPFALIPLLKFTSSK 420
           KLTPW+RNFLTR LAIVPSLIVAIIGGSSGAG+LIIIASMILSFELPFAL+PLLKFTSS+
Sbjct: 361 KLTPWVRNFLTRCLAIVPSLIVAIIGGSSGAGKLIIIASMILSFELPFALVPLLKFTSSR 420

Query: 421 TKMGPHVNSTAITVLTWIIGFLIMAINIYYLITRFIHVLLHNDLQLVAVVFIGILGFSGM 480
           TKMG HVNSTAITVLTWIIG LIMAINIYYL+TRFIHVLLHNDLQLVAVVFIGILGFSGM
Sbjct: 421 TKMGTHVNSTAITVLTWIIGCLIMAINIYYLMTRFIHVLLHNDLQLVAVVFIGILGFSGM 480

Query: 481 ALYLAGIAYLVLRKTKEITHFLALTTEESQRLSNEPSKTSGYNLPSEDIEIM 533
           A+YLAGIAYLV RKTKE TH LALTTEESQ+LSN PSK SGY LP+ED+  M
Sbjct: 481 AIYLAGIAYLVFRKTKESTHLLALTTEESQQLSNTPSKRSGYGLPNEDLTSM 532

BLAST of Lag0031241 vs. NCBI nr
Match: XP_022935446.1 (metal transporter Nramp6-like [Cucurbita moschata])

HSP 1 Score: 967.6 bits (2500), Expect = 4.5e-278
Identity = 502/532 (94.36%), Postives = 519/532 (97.56%), Query Frame = 0

Query: 1   MAAGGSNSGQPQFLARAGDESFSHAPLIENPETDQIVVPDKKSWKNLFAYMGPGFLVSIA 60
           MAAGGS+SGQPQF+ARAG+ESFSHAPLIENPETDQIVVPDKKSWKNLFAYMGPGFLVSIA
Sbjct: 1   MAAGGSSSGQPQFIARAGNESFSHAPLIENPETDQIVVPDKKSWKNLFAYMGPGFLVSIA 60

Query: 61  YIDPGNFETDLQSGAQYKYGLLWIILVASCAALIIQSLAANLGVVTGKHLAEHCKSEYPK 120
           YIDPGNFETDLQSGAQYKY LLWIILVASCAALIIQSLAANLGVVTGKHLAEHCK+EYPK
Sbjct: 61  YIDPGNFETDLQSGAQYKYELLWIILVASCAALIIQSLAANLGVVTGKHLAEHCKAEYPK 120

Query: 121 VQNFILWILAEIAIVACDIPEVIGTAFALNMLFSIPVWCGVLLTGLSTLFLLALQQYGIR 180
           VQNFILW+LAEIAIVACDIPEVIGTAFALNMLFSIPVWCGVLLTGLSTLFLLALQQYGIR
Sbjct: 121 VQNFILWVLAEIAIVACDIPEVIGTAFALNMLFSIPVWCGVLLTGLSTLFLLALQQYGIR 180

Query: 181 KLEFLIAFLVLTIAICFFLELGYAKPDVGEIFYGLFVPQLKGSGATGLAISLLGAMVMPH 240
           KLEFLIAFLVLTIA+CFFLELGYAKPDVGE+FYGLFVPQLKGSGATGLAISLLGAMVMPH
Sbjct: 181 KLEFLIAFLVLTIAVCFFLELGYAKPDVGEVFYGLFVPQLKGSGATGLAISLLGAMVMPH 240

Query: 241 NLFLHSALVLSRKIPRSVSGIKEACRFYLIESGFALMVAFFINVSVISVSGAVCNSPDLN 300
           NLFLHSALVLSRKIPRSVSGIKEACRFY+IESGFALMVAF INVSVISVSGAVCNSPDLN
Sbjct: 241 NLFLHSALVLSRKIPRSVSGIKEACRFYMIESGFALMVAFLINVSVISVSGAVCNSPDLN 300

Query: 301 KEDQMSCNDLDLNKASFLLRNVLGKWSSKLFAIALLASGQSSTITGTYAGQYVMQGFLDL 360
           KEDQMSCNDLDLNKASFLLRNVLGKWSSKLFAIALLASGQSSTITGTYAGQYVMQGFLDL
Sbjct: 301 KEDQMSCNDLDLNKASFLLRNVLGKWSSKLFAIALLASGQSSTITGTYAGQYVMQGFLDL 360

Query: 361 KLTPWIRNFLTRSLAIVPSLIVAIIGGSSGAGQLIIIASMILSFELPFALIPLLKFTSSK 420
           KLTPW+RNFLTR LAIVPSLIVAIIGGSSGAG+LIIIASMILSFELPFAL+PLLKFTSS+
Sbjct: 361 KLTPWVRNFLTRCLAIVPSLIVAIIGGSSGAGKLIIIASMILSFELPFALVPLLKFTSSR 420

Query: 421 TKMGPHVNSTAITVLTWIIGFLIMAINIYYLITRFIHVLLHNDLQLVAVVFIGILGFSGM 480
           TKMG HVNSTAITVLTWIIG LIMAINIYYL+TRFIHVLLHNDLQLVAVVFIGILGFSGM
Sbjct: 421 TKMGTHVNSTAITVLTWIIGCLIMAINIYYLMTRFIHVLLHNDLQLVAVVFIGILGFSGM 480

Query: 481 ALYLAGIAYLVLRKTKEITHFLALTTEESQRLSNEPSKTSGYNLPSEDIEIM 533
           A+YLAGIAYLV RKTKE TH LALTTEESQ+LSN PSK SGY LP+ED+  M
Sbjct: 481 AIYLAGIAYLVFRKTKESTHLLALTTEESQQLSNTPSKRSGYGLPNEDLTSM 532

BLAST of Lag0031241 vs. NCBI nr
Match: XP_023528489.1 (metal transporter Nramp6-like [Cucurbita pepo subsp. pepo] >XP_023528490.1 metal transporter Nramp6-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 964.5 bits (2492), Expect = 3.8e-277
Identity = 502/532 (94.36%), Postives = 517/532 (97.18%), Query Frame = 0

Query: 1   MAAGGSNSGQPQFLARAGDESFSHAPLIENPETDQIVVPDKKSWKNLFAYMGPGFLVSIA 60
           MAAGGS+SGQPQF+ARAG+ESFSHAPLIEN ETDQIVVPDKKSWKNLFAYMGPGFLVSIA
Sbjct: 1   MAAGGSSSGQPQFIARAGNESFSHAPLIENSETDQIVVPDKKSWKNLFAYMGPGFLVSIA 60

Query: 61  YIDPGNFETDLQSGAQYKYGLLWIILVASCAALIIQSLAANLGVVTGKHLAEHCKSEYPK 120
           YIDPGNFETDLQSGAQYKYGLLWIILVASCAALIIQSLAANLGVVTGKHLAEHCK+EYPK
Sbjct: 61  YIDPGNFETDLQSGAQYKYGLLWIILVASCAALIIQSLAANLGVVTGKHLAEHCKAEYPK 120

Query: 121 VQNFILWILAEIAIVACDIPEVIGTAFALNMLFSIPVWCGVLLTGLSTLFLLALQQYGIR 180
           VQNFILW+LAEIAIVACDIPEVIGTAFALNMLFSIPVWCGVLLTGLSTLFLLALQQYGIR
Sbjct: 121 VQNFILWVLAEIAIVACDIPEVIGTAFALNMLFSIPVWCGVLLTGLSTLFLLALQQYGIR 180

Query: 181 KLEFLIAFLVLTIAICFFLELGYAKPDVGEIFYGLFVPQLKGSGATGLAISLLGAMVMPH 240
           KLEFLIAFLVLTIAICFFLELGYAKPDVGE+FYGLFVPQLKGSGATGLAISLLGAMVMPH
Sbjct: 181 KLEFLIAFLVLTIAICFFLELGYAKPDVGEVFYGLFVPQLKGSGATGLAISLLGAMVMPH 240

Query: 241 NLFLHSALVLSRKIPRSVSGIKEACRFYLIESGFALMVAFFINVSVISVSGAVCNSPDLN 300
           NLFLHSALVLSRKIPRSVSGIKEACRFY+IESGFALMVAF INVSVISVSGAVCNSPDLN
Sbjct: 241 NLFLHSALVLSRKIPRSVSGIKEACRFYMIESGFALMVAFLINVSVISVSGAVCNSPDLN 300

Query: 301 KEDQMSCNDLDLNKASFLLRNVLGKWSSKLFAIALLASGQSSTITGTYAGQYVMQGFLDL 360
            EDQMSCNDLDLNKASFLLRNVLGKWSSKLFAIALLASGQSSTITGTYAGQYVMQGFLDL
Sbjct: 301 MEDQMSCNDLDLNKASFLLRNVLGKWSSKLFAIALLASGQSSTITGTYAGQYVMQGFLDL 360

Query: 361 KLTPWIRNFLTRSLAIVPSLIVAIIGGSSGAGQLIIIASMILSFELPFALIPLLKFTSSK 420
           KLTPW+RNFLTR LAIVPSLIVAIIGGSSGAG+LIIIASMILSFELPFALIPLLKFTSS+
Sbjct: 361 KLTPWVRNFLTRCLAIVPSLIVAIIGGSSGAGKLIIIASMILSFELPFALIPLLKFTSSR 420

Query: 421 TKMGPHVNSTAITVLTWIIGFLIMAINIYYLITRFIHVLLHNDLQLVAVVFIGILGFSGM 480
           TKMG HVNSTAITVLTWIIG LIMAINIYYL+TRFIHVLLHNDLQLVAVVFIGILGFSGM
Sbjct: 421 TKMGTHVNSTAITVLTWIIGCLIMAINIYYLMTRFIHVLLHNDLQLVAVVFIGILGFSGM 480

Query: 481 ALYLAGIAYLVLRKTKEITHFLALTTEESQRLSNEPSKTSGYNLPSEDIEIM 533
           A+YLAGI YLV RKTKE TH LALTTEESQ+LSN PSK SGY LP+ED+  M
Sbjct: 481 AIYLAGITYLVFRKTKESTHLLALTTEESQQLSNTPSKRSGYGLPNEDLTSM 532

BLAST of Lag0031241 vs. ExPASy Swiss-Prot
Match: Q9S9N8 (Metal transporter Nramp6 OS=Arabidopsis thaliana OX=3702 GN=NRAMP6 PE=2 SV=2)

HSP 1 Score: 778.9 bits (2010), Expect = 3.9e-224
Identity = 405/520 (77.88%), Postives = 458/520 (88.08%), Query Frame = 0

Query: 15  ARAGDESFSHAPLIENPETDQIVVPDKKSWKNLFAYMGPGFLVSIAYIDPGNFETDLQSG 74
           A   + S S++PLIEN +++QI+VP+KKSWKN F+Y+GPGFLVSIAYIDPGNFETDLQSG
Sbjct: 6   ASGSNRSISNSPLIENSDSNQILVPEKKSWKNFFSYLGPGFLVSIAYIDPGNFETDLQSG 65

Query: 75  AQYKYGLLWIILVASCAALIIQSLAANLGVVTGKHLAEHCKSEYPKVQNFILWILAEIAI 134
           AQYKY LLWIILVASCAAL+IQSLAANLGVVTGKHLAEHC++EY KV NF+LW++AEIA+
Sbjct: 66  AQYKYELLWIILVASCAALVIQSLAANLGVVTGKHLAEHCRAEYSKVPNFMLWVVAEIAV 125

Query: 135 VACDIPEVIGTAFALNMLFSIPVWCGVLLTGLSTLFLLALQQYGIRKLEFLIAFLVLTIA 194
           VACDIPEVIGTAFALNMLF+IPVW GVLLTGLSTL LLALQQYGIRKLEFLIAFLV TIA
Sbjct: 126 VACDIPEVIGTAFALNMLFNIPVWIGVLLTGLSTLILLALQQYGIRKLEFLIAFLVFTIA 185

Query: 195 ICFFLELGYAKPDVGEIFYGLFVPQLKGSGATGLAISLLGAMVMPHNLFLHSALVLSRKI 254
           +CFF+EL Y+KPD  E+ YGLFVPQLKG+GATGLAISLLGAMVMPHNLFLHSALVLSRKI
Sbjct: 186 LCFFVELHYSKPDPKEVLYGLFVPQLKGNGATGLAISLLGAMVMPHNLFLHSALVLSRKI 245

Query: 255 PRSVSGIKEACRFYLIESGFALMVAFFINVSVISVSGAVCNSPDLNKEDQMSCNDLDLNK 314
           PRSV+GIKEACR+YLIESG ALMVAF INVSVISVSGAVCN+ DL+ ED+ SC DLDLNK
Sbjct: 246 PRSVTGIKEACRYYLIESGLALMVAFLINVSVISVSGAVCNASDLSPEDRASCQDLDLNK 305

Query: 315 ASFLLRNVLGKWSSKLFAIALLASGQSSTITGTYAGQYVMQGFLDLKLTPWIRNFLTRSL 374
           ASFLLRNV+GKWSSKLFAIALLASGQSSTITGTYAGQYVMQGFLDL+L PW+RNFLTR L
Sbjct: 306 ASFLLRNVVGKWSSKLFAIALLASGQSSTITGTYAGQYVMQGFLDLRLEPWLRNFLTRCL 365

Query: 375 AIVPSLIVAIIGGSSGAGQLIIIASMILSFELPFALIPLLKFTSSKTKMGPHVNSTAITV 434
           AI+PSLIVA+IGGS+GAG+LIIIASMILSFELPFAL+PLLKFTSSKTKMG H NS  I+ 
Sbjct: 366 AIIPSLIVALIGGSAGAGKLIIIASMILSFELPFALVPLLKFTSSKTKMGSHANSLVISS 425

Query: 435 LTWIIGFLIMAINIYYLITRFIHVLLHNDLQLVAVVFIGILGFSGMALYLAGIAYLVLRK 494
           +TWIIG LIM INIYYL++ FI +LLH+ + LVA+VF+G+LGFSG+A YLA I+YLVLRK
Sbjct: 426 VTWIIGGLIMGINIYYLVSSFIKLLLHSHMNLVAIVFLGVLGFSGIATYLAAISYLVLRK 485

Query: 495 TKE--ITHFLALTTEESQRLSNEPSKTSGYNLPSEDIEIM 533
            +E   THFL  +  +++             LP EDI  M
Sbjct: 486 NRESSSTHFLDFSNSQTEE-----------TLPREDIANM 514

BLAST of Lag0031241 vs. ExPASy Swiss-Prot
Match: Q9SAH8 (Metal transporter Nramp1 OS=Arabidopsis thaliana OX=3702 GN=NRAMP1 PE=1 SV=1)

HSP 1 Score: 774.2 bits (1998), Expect = 9.5e-223
Identity = 404/517 (78.14%), Postives = 460/517 (88.97%), Query Frame = 0

Query: 1   MAAGGSNSGQPQFLARA-GDESFSHAPLIENPETDQIVVPDKKSWKNLFAYMGPGFLVSI 60
           MAA G  SG+ QF++ + G+ SFS++PLIEN +++QI+V +KKSWKN FAY+GPGFLVSI
Sbjct: 1   MAATG--SGRSQFISSSGGNRSFSNSPLIENSDSNQIIVSEKKSWKNFFAYLGPGFLVSI 60

Query: 61  AYIDPGNFETDLQSGAQYKYGLLWIILVASCAALIIQSLAANLGVVTGKHLAEHCKSEYP 120
           AYIDPGNFETDLQ+GA YKY LLWIILVASCAAL+IQSLAANLGVVTGKHLAE C++EY 
Sbjct: 61  AYIDPGNFETDLQAGAHYKYELLWIILVASCAALVIQSLAANLGVVTGKHLAEQCRAEYS 120

Query: 121 KVQNFILWILAEIAIVACDIPEVIGTAFALNMLFSIPVWCGVLLTGLSTLFLLALQQYGI 180
           KV NF+LW++AEIA+VACDIPEVIGTAFALNMLFSIPVW GVLLTGLSTL LLALQ+YG+
Sbjct: 121 KVPNFMLWVVAEIAVVACDIPEVIGTAFALNMLFSIPVWIGVLLTGLSTLILLALQKYGV 180

Query: 181 RKLEFLIAFLVLTIAICFFLELGYAKPDVGEIFYGLFVPQLKGSGATGLAISLLGAMVMP 240
           RKLEFLIAFLV TIAICFF+EL Y+KPD GE+ +GLFVPQLKG+GATGLAISLLGAMVMP
Sbjct: 181 RKLEFLIAFLVFTIAICFFVELHYSKPDPGEVLHGLFVPQLKGNGATGLAISLLGAMVMP 240

Query: 241 HNLFLHSALVLSRKIPRSVSGIKEACRFYLIESGFALMVAFFINVSVISVSGAVCNSPDL 300
           HNLFLHSALVLSRKIPRS SGIKEACRFYLIESG ALMVAF INVSVISVSGAVCN+P+L
Sbjct: 241 HNLFLHSALVLSRKIPRSASGIKEACRFYLIESGLALMVAFLINVSVISVSGAVCNAPNL 300

Query: 301 NKEDQMSCNDLDLNKASFLLRNVLGKWSSKLFAIALLASGQSSTITGTYAGQYVMQGFLD 360
           + ED+ +C DLDLNKASFLLRNV+GKWSSKLFAIALLASGQSSTITGTYAGQYVMQGFLD
Sbjct: 301 SPEDRANCEDLDLNKASFLLRNVVGKWSSKLFAIALLASGQSSTITGTYAGQYVMQGFLD 360

Query: 361 LKLTPWIRNFLTRSLAIVPSLIVAIIGGSSGAGQLIIIASMILSFELPFALIPLLKFTSS 420
           L+L PW+RN LTR LAI+PSLIVA+IGGS+GAG+LIIIASMILSFELPFAL+PLLKFTS 
Sbjct: 361 LRLEPWLRNLLTRCLAIIPSLIVALIGGSAGAGKLIIIASMILSFELPFALVPLLKFTSC 420

Query: 421 KTKMGPHVNSTAITVLTWIIGFLIMAINIYYLITRFIHVLLHNDLQLVAVVFIGILGFSG 480
           KTKMG HVN  AIT LTW+IG LIM INIYYL++ FI +L+H+ ++L+ VVF GILGF+G
Sbjct: 421 KTKMGSHVNPMAITALTWVIGGLIMGINIYYLVSSFIKLLIHSHMKLILVVFCGILGFAG 480

Query: 481 MALYLAGIAYLVLRKTKEITHFLALTTEESQRLSNEP 517
           +ALYLA IAYLV RK +  T    L + +SQ +   P
Sbjct: 481 IALYLAAIAYLVFRKNRVATSL--LISRDSQNVETLP 513

BLAST of Lag0031241 vs. ExPASy Swiss-Prot
Match: Q653V6 (Metal transporter Nramp3 OS=Oryza sativa subsp. japonica OX=39947 GN=NRAMP3 PE=2 SV=1)

HSP 1 Score: 743.8 bits (1919), Expect = 1.4e-213
Identity = 391/539 (72.54%), Postives = 449/539 (83.30%), Query Frame = 0

Query: 1   MAAGGSNSGQPQFLA---RAGDESFSHAPLIENPETDQIVVPDKKSWKNLFAYMGPGFLV 60
           M+     S QPQF++   R    +    PLI++ + DQIV+P+K SWKNLF+Y+GPGFLV
Sbjct: 1   MSGPMQRSSQPQFISSVERNNQSNGPGTPLIDSIDVDQIVIPEKNSWKNLFSYIGPGFLV 60

Query: 61  SIAYIDPGNFETDLQSGAQYKYGLLWIILVASCAALIIQSLAANLGVVTGKHLAEHCKSE 120
           SIAYIDPGNFETDLQ+GAQYKY LLWIIL+ASCAALIIQSLAA LGVVTGKHLAEHC++E
Sbjct: 61  SIAYIDPGNFETDLQAGAQYKYELLWIILIASCAALIIQSLAARLGVVTGKHLAEHCRAE 120

Query: 121 YPKVQNFILWILAEIAIVACDIPEVIGTAFALNMLFSIPVWCGVLLTGLSTLFLLALQQY 180
           YPK  NFILWILAE+A+VACDIPEVIGTAFALNMLF IPVWCGVL+TGLSTL LL LQQY
Sbjct: 121 YPKATNFILWILAELAVVACDIPEVIGTAFALNMLFKIPVWCGVLITGLSTLMLLLLQQY 180

Query: 181 GIRKLEFLIAFLVLTIAICFFLELGYAKPDVGEIFYGLFVPQLKGSGATGLAISLLGAMV 240
           G+RKLEFLIA LV  IA CF +ELGY+KP+  E+  GLFVP+LKG+GATGLAISLLGAMV
Sbjct: 181 GVRKLEFLIAILVSLIATCFLVELGYSKPNSSEVVRGLFVPELKGNGATGLAISLLGAMV 240

Query: 241 MPHNLFLHSALVLSRKIPRSVSGIKEACRFYLIESGFALMVAFFINVSVISVSGAVCNSP 300
           MPHNLFLHSALVLSRK+PRSV GIKEACRFY+IES FAL +AF IN+S+ISVSGAVC S 
Sbjct: 241 MPHNLFLHSALVLSRKVPRSVHGIKEACRFYMIESAFALTIAFLINISIISVSGAVCGSD 300

Query: 301 DLNKEDQMSCNDLDLNKASFLLRNVLGKWSSKLFAIALLASGQSSTITGTYAGQYVMQGF 360
           +L+ EDQM+C+DLDLNKASFLL+NVLG WSSKLFA+ALLASGQSSTITGTYAGQYVMQGF
Sbjct: 301 NLSPEDQMNCSDLDLNKASFLLKNVLGNWSSKLFAVALLASGQSSTITGTYAGQYVMQGF 360

Query: 361 LDLKLTPWIRNFLTRSLAIVPSLIVAIIGGSSGAGQLIIIASMILSFELPFALIPLLKFT 420
           LDL++TPWIRN LTRSLAI+PSLIV+IIGGSS AGQLIIIASMILSFELPFAL+PLLKFT
Sbjct: 361 LDLRMTPWIRNLLTRSLAILPSLIVSIIGGSSAAGQLIIIASMILSFELPFALVPLLKFT 420

Query: 421 SSKTKMGPHVNSTAITVLTWIIGFLIMAINIYYLITRFIHVLLHNDLQLVAVVFIGILGF 480
           SS+TKMG H NS AI+V+TW IG  I+ IN Y+LIT F+ +LLHN L  V+ VF GI GF
Sbjct: 421 SSRTKMGQHTNSKAISVITWGIGSFIVVINTYFLITSFVKLLLHNGLSTVSQVFSGIFGF 480

Query: 481 SGMALYLAGIAYLVLRKTKEITHFLALTTEESQRLSNEPSKTSGY----NLPSEDIEIM 533
            GM +Y+A I YLV RK ++ T  L L  + + R+    + T G     +LP EDI  M
Sbjct: 481 LGMLIYMAAILYLVFRKNRKATLPL-LEGDSTVRIVGRDTATEGEGSLGHLPREDISSM 538

BLAST of Lag0031241 vs. ExPASy Swiss-Prot
Match: Q8H4H5 (Metal transporter Nramp5 OS=Oryza sativa subsp. japonica OX=39947 GN=NRAMP5 PE=2 SV=1)

HSP 1 Score: 578.9 bits (1491), Expect = 5.9e-164
Identity = 296/479 (61.80%), Postives = 371/479 (77.45%), Query Frame = 0

Query: 21  SFSHAPLIENPETDQIVVPDKKSWKNLFAYMGPGFLVSIAYIDPGNFETDLQSGAQYKYG 80
           S +H    +  + D  ++  + +WK   A++GPGF+VS+AY+DPGN ETDLQ+GA ++Y 
Sbjct: 18  SAAHDQDAKKLDADDQLLMKEPAWKRFLAHVGPGFMVSLAYLDPGNLETDLQAGANHRYE 77

Query: 81  LLWIILVASCAALIIQSLAANLGVVTGKHLAEHCKSEYPKVQNFILWILAEIAIVACDIP 140
           LLW+IL+    ALIIQSLAANLGVVTG+HLAE CKSEYPK     LW+LAE+A++A DIP
Sbjct: 78  LLWVILIGLIFALIIQSLAANLGVVTGRHLAEICKSEYPKFVKIFLWLLAELAVIAADIP 137

Query: 141 EVIGTAFALNMLFSIPVWCGVLLTGLSTLFLLALQQYGIRKLEFLIAFLVLTIAICFFLE 200
           EVIGTAFA N+LF IPVW GVL+TG STL LL LQ+YG+RKLEFLI+ LV  +A CFF E
Sbjct: 138 EVIGTAFAFNILFHIPVWVGVLITGTSTLLLLGLQKYGVRKLEFLISMLVFVMAACFFGE 197

Query: 201 LGYAKPDVGEIFYGLFVPQLKGSGATGLAISLLGAMVMPHNLFLHSALVLSRKIPRSVSG 260
           L   KP   E+  GLF+P+L G GAT  AI+LLGA+VMPHNLFLHSALVLSRK P SV G
Sbjct: 198 LSIVKPPAKEVMKGLFIPRLNGDGATADAIALLGALVMPHNLFLHSALVLSRKTPASVRG 257

Query: 261 IKEACRFYLIESGFALMVAFFINVSVISVSGAVCNSPDLNKEDQMSCNDLDLNKASFLLR 320
           IK+ CRF+L ESGFAL VA  IN++V+SVSG  C+S +L++ED   C +L L+ +SFLL+
Sbjct: 258 IKDGCRFFLYESGFALFVALLINIAVVSVSGTACSSANLSQEDADKCANLSLDTSSFLLK 317

Query: 321 NVLGKWSSKLFAIALLASGQSSTITGTYAGQYVMQGFLDLKLTPWIRNFLTRSLAIVPSL 380
           NVLGK S+ ++ +ALLASGQSSTITGTYAGQY+MQGFLD+++  W+RN +TR++AI PSL
Sbjct: 318 NVLGKSSAIVYGVALLASGQSSTITGTYAGQYIMQGFLDIRMRKWLRNLMTRTIAIAPSL 377

Query: 381 IVAIIGGSSGAGQLIIIASMILSFELPFALIPLLKFTSSKTKMGPHVNSTAITVLTWIIG 440
           IV+IIGGS GAG+LIIIASMILSFELPFALIPLLKF+SSK+KMGPH NS  I V +W +G
Sbjct: 378 IVSIIGGSRGAGRLIIIASMILSFELPFALIPLLKFSSSKSKMGPHKNSIYIIVFSWFLG 437

Query: 441 FLIMAINIYYLITRFIHVLLHNDLQLVAVVFIGILGFSGMALYLAGIAYLVLRKTKEIT 500
            LI+ IN+Y+L T F+  L+HNDL   A V +G   F  M +Y+  + YL +RK   +T
Sbjct: 438 LLIIGINMYFLSTSFVGWLIHNDLPKYANVLVGAAVFPFMLVYIVAVVYLTIRKDSVVT 496

BLAST of Lag0031241 vs. ExPASy Swiss-Prot
Match: Q0D7E4 (Metal transporter Nramp1 OS=Oryza sativa subsp. japonica OX=39947 GN=NRAMP1 PE=2 SV=1)

HSP 1 Score: 560.1 bits (1442), Expect = 2.8e-158
Identity = 284/452 (62.83%), Postives = 357/452 (78.98%), Query Frame = 0

Query: 43  SWKNLFAYMGPGFLVSIAYIDPGNFETDLQSGAQYKYGLLWIILVASCAALIIQSLAANL 102
           +WK   +++GPGF+V +AY+DPGN ETDLQ+GA +KY LLW+IL+    ALIIQSL+ANL
Sbjct: 31  AWKRFLSHIGPGFMVCLAYLDPGNMETDLQAGANHKYELLWVILIGLIFALIIQSLSANL 90

Query: 103 GVVTGKHLAEHCKSEYPKVQNFILWILAEIAIVACDIPEVIGTAFALNMLFSIPVWCGVL 162
           GVVTG+HLAE CK+EYP      LW+LAE+A++A DIPEVIGT FA N+LF IPVW GVL
Sbjct: 91  GVVTGRHLAELCKTEYPVWVKTCLWLLAELAVIASDIPEVIGTGFAFNLLFHIPVWTGVL 150

Query: 163 LTGLSTLFLLALQQYGIRKLEFLIAFLVLTIAICFFLELGYAKPDVGEIFYGLFVPQLKG 222
           + G STL LL LQ+YG+RKLE ++A LV  +A CFF+E+   KP V E+  GLF+P+L G
Sbjct: 151 IAGSSTLLLLGLQRYGVRKLEVVVALLVFVMAGCFFVEMSIVKPPVNEVLQGLFIPRLSG 210

Query: 223 SGATGLAISLLGAMVMPHNLFLHSALVLSRKIPRSVSGIKEACRFYLIESGFALMVAFFI 282
            GATG +I+LLGA+VMPHNLFLHSALVLSR  P S  G+K+ CRF+L ESG AL VA  +
Sbjct: 211 PGATGDSIALLGALVMPHNLFLHSALVLSRNTPASAKGMKDVCRFFLFESGIALFVALLV 270

Query: 283 NVSVISVSGAVCNSPDLNKEDQMSCNDLDLNKASFLLRNVLGKWSSKLFAIALLASGQSS 342
           N+++ISVSG VCN+ +L+ ED + C+DL L+ +SFLLRNVLGK S+ ++ +ALLASGQSS
Sbjct: 271 NIAIISVSGTVCNATNLSPEDAVKCSDLTLDSSSFLLRNVLGKSSATVYGVALLASGQSS 330

Query: 343 TITGTYAGQYVMQGFLDLKLTPWIRNFLTRSLAIVPSLIVAIIGGSSGAGQLIIIASMIL 402
           TITGTYAGQYVMQGFLD+K+  W+RN +TRS+AIVPSLIV+IIGGSSGAG+LI+IASMIL
Sbjct: 331 TITGTYAGQYVMQGFLDIKMKQWLRNLMTRSIAIVPSLIVSIIGGSSGAGRLIVIASMIL 390

Query: 403 SFELPFALIPLLKFTSSKTKMGPHVNSTAITVLTWIIGFLIMAINIYYLITRFIHVLLHN 462
           SFELPFALIPLLKF+SS  KMG + NS  I   +W++GF+I+ INIY+L T+ +  +LHN
Sbjct: 391 SFELPFALIPLLKFSSSSNKMGENKNSIYIVGFSWVLGFVIIGINIYFLSTKLVGWILHN 450

Query: 463 DLQLVAVVFIGILGFSGMALYLAGIAYLVLRK 495
            L   A V IGI+ F  M LY+  + YL  RK
Sbjct: 451 ALPTFANVLIGIVLFPLMLLYVVAVIYLTFRK 482

BLAST of Lag0031241 vs. ExPASy TrEMBL
Match: A0A6J1J3T2 (metal transporter Nramp6-like OS=Cucurbita maxima OX=3661 GN=LOC111481537 PE=3 SV=1)

HSP 1 Score: 970.3 bits (2507), Expect = 3.3e-279
Identity = 503/529 (95.09%), Postives = 519/529 (98.11%), Query Frame = 0

Query: 1   MAAGGSNSGQPQFLARAGDESFSHAPLIENPETDQIVVPDKKSWKNLFAYMGPGFLVSIA 60
           MAAGGS+SGQPQF+ARAG+ESFSHAPLIENPET QIVVPDKKSWKNLFAYMGPGFLVSIA
Sbjct: 1   MAAGGSSSGQPQFIARAGNESFSHAPLIENPETGQIVVPDKKSWKNLFAYMGPGFLVSIA 60

Query: 61  YIDPGNFETDLQSGAQYKYGLLWIILVASCAALIIQSLAANLGVVTGKHLAEHCKSEYPK 120
           YIDPGNFETDLQSGAQYKYGLLWIILVASCAALIIQSLAANLGVVTGKHLAEHCK+EYPK
Sbjct: 61  YIDPGNFETDLQSGAQYKYGLLWIILVASCAALIIQSLAANLGVVTGKHLAEHCKAEYPK 120

Query: 121 VQNFILWILAEIAIVACDIPEVIGTAFALNMLFSIPVWCGVLLTGLSTLFLLALQQYGIR 180
           VQNFILW+LAEIAIVACDIPEVIGTAFALNMLFSIPVWCGVLLTGLSTLFLLALQQYGIR
Sbjct: 121 VQNFILWVLAEIAIVACDIPEVIGTAFALNMLFSIPVWCGVLLTGLSTLFLLALQQYGIR 180

Query: 181 KLEFLIAFLVLTIAICFFLELGYAKPDVGEIFYGLFVPQLKGSGATGLAISLLGAMVMPH 240
           KLEFLIAFLVLTIAICFFLELGYAKPDVGE+FYGLFVPQLKGSGATGLAISLLGAMVMPH
Sbjct: 181 KLEFLIAFLVLTIAICFFLELGYAKPDVGEVFYGLFVPQLKGSGATGLAISLLGAMVMPH 240

Query: 241 NLFLHSALVLSRKIPRSVSGIKEACRFYLIESGFALMVAFFINVSVISVSGAVCNSPDLN 300
           NLFLHSALVLSRKIPRSVSGIKEACRFY+IESGFALMVAF INVSVISVSGAVCNSPDLN
Sbjct: 241 NLFLHSALVLSRKIPRSVSGIKEACRFYMIESGFALMVAFLINVSVISVSGAVCNSPDLN 300

Query: 301 KEDQMSCNDLDLNKASFLLRNVLGKWSSKLFAIALLASGQSSTITGTYAGQYVMQGFLDL 360
           KEDQMSCNDLDLNKASFLLRNVLGKWSSKLFAIALLASGQSSTITGTYAGQYVMQGFLDL
Sbjct: 301 KEDQMSCNDLDLNKASFLLRNVLGKWSSKLFAIALLASGQSSTITGTYAGQYVMQGFLDL 360

Query: 361 KLTPWIRNFLTRSLAIVPSLIVAIIGGSSGAGQLIIIASMILSFELPFALIPLLKFTSSK 420
           KLTPW+RNFLTR LAIVPSLIVAIIGGSSGAG+LIIIASMILSFELPFAL+PLLKFTSS+
Sbjct: 361 KLTPWVRNFLTRCLAIVPSLIVAIIGGSSGAGKLIIIASMILSFELPFALVPLLKFTSSR 420

Query: 421 TKMGPHVNSTAITVLTWIIGFLIMAINIYYLITRFIHVLLHNDLQLVAVVFIGILGFSGM 480
           TKMG HVNSTAITVLTWIIGFLIMAINIYYL+TRFIHVLLHNDLQLVAVVFIGILGFSGM
Sbjct: 421 TKMGTHVNSTAITVLTWIIGFLIMAINIYYLMTRFIHVLLHNDLQLVAVVFIGILGFSGM 480

Query: 481 ALYLAGIAYLVLRKTKEITHFLALTTEESQRLSNEPSKTSGYNLPSEDI 530
           A+YLAGIAYLV RKTKE TH LALTTEESQ+LSN PSK SGY LP+ED+
Sbjct: 481 AIYLAGIAYLVFRKTKESTHLLALTTEESQQLSNTPSKRSGYGLPNEDL 529

BLAST of Lag0031241 vs. ExPASy TrEMBL
Match: A0A6J1F4P3 (metal transporter Nramp6-like OS=Cucurbita moschata OX=3662 GN=LOC111442317 PE=3 SV=1)

HSP 1 Score: 967.6 bits (2500), Expect = 2.2e-278
Identity = 502/532 (94.36%), Postives = 519/532 (97.56%), Query Frame = 0

Query: 1   MAAGGSNSGQPQFLARAGDESFSHAPLIENPETDQIVVPDKKSWKNLFAYMGPGFLVSIA 60
           MAAGGS+SGQPQF+ARAG+ESFSHAPLIENPETDQIVVPDKKSWKNLFAYMGPGFLVSIA
Sbjct: 1   MAAGGSSSGQPQFIARAGNESFSHAPLIENPETDQIVVPDKKSWKNLFAYMGPGFLVSIA 60

Query: 61  YIDPGNFETDLQSGAQYKYGLLWIILVASCAALIIQSLAANLGVVTGKHLAEHCKSEYPK 120
           YIDPGNFETDLQSGAQYKY LLWIILVASCAALIIQSLAANLGVVTGKHLAEHCK+EYPK
Sbjct: 61  YIDPGNFETDLQSGAQYKYELLWIILVASCAALIIQSLAANLGVVTGKHLAEHCKAEYPK 120

Query: 121 VQNFILWILAEIAIVACDIPEVIGTAFALNMLFSIPVWCGVLLTGLSTLFLLALQQYGIR 180
           VQNFILW+LAEIAIVACDIPEVIGTAFALNMLFSIPVWCGVLLTGLSTLFLLALQQYGIR
Sbjct: 121 VQNFILWVLAEIAIVACDIPEVIGTAFALNMLFSIPVWCGVLLTGLSTLFLLALQQYGIR 180

Query: 181 KLEFLIAFLVLTIAICFFLELGYAKPDVGEIFYGLFVPQLKGSGATGLAISLLGAMVMPH 240
           KLEFLIAFLVLTIA+CFFLELGYAKPDVGE+FYGLFVPQLKGSGATGLAISLLGAMVMPH
Sbjct: 181 KLEFLIAFLVLTIAVCFFLELGYAKPDVGEVFYGLFVPQLKGSGATGLAISLLGAMVMPH 240

Query: 241 NLFLHSALVLSRKIPRSVSGIKEACRFYLIESGFALMVAFFINVSVISVSGAVCNSPDLN 300
           NLFLHSALVLSRKIPRSVSGIKEACRFY+IESGFALMVAF INVSVISVSGAVCNSPDLN
Sbjct: 241 NLFLHSALVLSRKIPRSVSGIKEACRFYMIESGFALMVAFLINVSVISVSGAVCNSPDLN 300

Query: 301 KEDQMSCNDLDLNKASFLLRNVLGKWSSKLFAIALLASGQSSTITGTYAGQYVMQGFLDL 360
           KEDQMSCNDLDLNKASFLLRNVLGKWSSKLFAIALLASGQSSTITGTYAGQYVMQGFLDL
Sbjct: 301 KEDQMSCNDLDLNKASFLLRNVLGKWSSKLFAIALLASGQSSTITGTYAGQYVMQGFLDL 360

Query: 361 KLTPWIRNFLTRSLAIVPSLIVAIIGGSSGAGQLIIIASMILSFELPFALIPLLKFTSSK 420
           KLTPW+RNFLTR LAIVPSLIVAIIGGSSGAG+LIIIASMILSFELPFAL+PLLKFTSS+
Sbjct: 361 KLTPWVRNFLTRCLAIVPSLIVAIIGGSSGAGKLIIIASMILSFELPFALVPLLKFTSSR 420

Query: 421 TKMGPHVNSTAITVLTWIIGFLIMAINIYYLITRFIHVLLHNDLQLVAVVFIGILGFSGM 480
           TKMG HVNSTAITVLTWIIG LIMAINIYYL+TRFIHVLLHNDLQLVAVVFIGILGFSGM
Sbjct: 421 TKMGTHVNSTAITVLTWIIGCLIMAINIYYLMTRFIHVLLHNDLQLVAVVFIGILGFSGM 480

Query: 481 ALYLAGIAYLVLRKTKEITHFLALTTEESQRLSNEPSKTSGYNLPSEDIEIM 533
           A+YLAGIAYLV RKTKE TH LALTTEESQ+LSN PSK SGY LP+ED+  M
Sbjct: 481 AIYLAGIAYLVFRKTKESTHLLALTTEESQQLSNTPSKRSGYGLPNEDLTSM 532

BLAST of Lag0031241 vs. ExPASy TrEMBL
Match: A0A5A7T2K9 (Metal transporter Nramp6 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold1593G00280 PE=3 SV=1)

HSP 1 Score: 963.8 bits (2490), Expect = 3.1e-277
Identity = 500/532 (93.98%), Postives = 519/532 (97.56%), Query Frame = 0

Query: 1   MAAGGSNSGQPQFLARAGDESFSHAPLIENPETDQIVVPDKKSWKNLFAYMGPGFLVSIA 60
           MAAGGS SGQPQFL RAGD+SFSHAPLIENPETDQI+VPDK+SWKNLFAYMGPGFLVSIA
Sbjct: 1   MAAGGSGSGQPQFLVRAGDDSFSHAPLIENPETDQIIVPDKESWKNLFAYMGPGFLVSIA 60

Query: 61  YIDPGNFETDLQSGAQYKYGLLWIILVASCAALIIQSLAANLGVVTGKHLAEHCKSEYPK 120
           YIDPGNFETDLQSGAQYKYGLLWIILVAS AALIIQSLAANLGVVTGKHLAEHCK+EYPK
Sbjct: 61  YIDPGNFETDLQSGAQYKYGLLWIILVASFAALIIQSLAANLGVVTGKHLAEHCKAEYPK 120

Query: 121 VQNFILWILAEIAIVACDIPEVIGTAFALNMLFSIPVWCGVLLTGLSTLFLLALQQYGIR 180
            QNFILW+LAEIAIVACDIPEVIGTAFALNMLF IPVWCGVLLTGLSTL LLALQQYGIR
Sbjct: 121 AQNFILWVLAEIAIVACDIPEVIGTAFALNMLFGIPVWCGVLLTGLSTLLLLALQQYGIR 180

Query: 181 KLEFLIAFLVLTIAICFFLELGYAKPDVGEIFYGLFVPQLKGSGATGLAISLLGAMVMPH 240
           KLEFLIAFLVLTIAICFFLELGYAKPDVGEIFYGLFVPQLKGSGATGLAISLLGAMVMPH
Sbjct: 181 KLEFLIAFLVLTIAICFFLELGYAKPDVGEIFYGLFVPQLKGSGATGLAISLLGAMVMPH 240

Query: 241 NLFLHSALVLSRKIPRSVSGIKEACRFYLIESGFALMVAFFINVSVISVSGAVCNSPDLN 300
           NLFLHSALVLSRKIPRS+SGIKEACRFY+IESGFALMVAF INVSVISVSGAVCN+PDLN
Sbjct: 241 NLFLHSALVLSRKIPRSLSGIKEACRFYMIESGFALMVAFLINVSVISVSGAVCNAPDLN 300

Query: 301 KEDQMSCNDLDLNKASFLLRNVLGKWSSKLFAIALLASGQSSTITGTYAGQYVMQGFLDL 360
           KEDQMSCNDLDLNKASFLLRNVLGKWSSKLFAIALLASGQSSTITGTYAGQYVMQGFLDL
Sbjct: 301 KEDQMSCNDLDLNKASFLLRNVLGKWSSKLFAIALLASGQSSTITGTYAGQYVMQGFLDL 360

Query: 361 KLTPWIRNFLTRSLAIVPSLIVAIIGGSSGAGQLIIIASMILSFELPFALIPLLKFTSSK 420
           KLTPWIRNFLTRSLAIVPSLIVAIIGGSSGAG+LIIIASMILSFELPFAL+PLLKFTSSK
Sbjct: 361 KLTPWIRNFLTRSLAIVPSLIVAIIGGSSGAGKLIIIASMILSFELPFALVPLLKFTSSK 420

Query: 421 TKMGPHVNSTAITVLTWIIGFLIMAINIYYLITRFIHVLLHNDLQLVAVVFIGILGFSGM 480
            KMGPHVNSTAITVLTWIIGFLIMAINIYYL++RFIHVLLHN+L L AVVFIGILGFSG+
Sbjct: 421 AKMGPHVNSTAITVLTWIIGFLIMAINIYYLMSRFIHVLLHNNLHLAAVVFIGILGFSGV 480

Query: 481 ALYLAGIAYLVLRKTKEITHFLALTTEESQRLSNEPSKTSGYNLPSEDIEIM 533
           ALYLAGIAYLVLRKTKEI+H LALTTEES+RLSN+PSKTSGY+LP+EDI  M
Sbjct: 481 ALYLAGIAYLVLRKTKEISHLLALTTEESRRLSNDPSKTSGYDLPNEDIVSM 532

BLAST of Lag0031241 vs. ExPASy TrEMBL
Match: A0A5D3DUP0 (Metal transporter Nramp6 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold901G00300 PE=3 SV=1)

HSP 1 Score: 963.8 bits (2490), Expect = 3.1e-277
Identity = 500/532 (93.98%), Postives = 519/532 (97.56%), Query Frame = 0

Query: 1   MAAGGSNSGQPQFLARAGDESFSHAPLIENPETDQIVVPDKKSWKNLFAYMGPGFLVSIA 60
           MAAGGS SGQPQFL RAGD+SFSHAPLIENPETDQI+VPDK+SWKNLFAYMGPGFLVSIA
Sbjct: 25  MAAGGSGSGQPQFLVRAGDDSFSHAPLIENPETDQIIVPDKESWKNLFAYMGPGFLVSIA 84

Query: 61  YIDPGNFETDLQSGAQYKYGLLWIILVASCAALIIQSLAANLGVVTGKHLAEHCKSEYPK 120
           YIDPGNFETDLQSGAQYKYGLLWIILVAS AALIIQSLAANLGVVTGKHLAEHCK+EYPK
Sbjct: 85  YIDPGNFETDLQSGAQYKYGLLWIILVASFAALIIQSLAANLGVVTGKHLAEHCKAEYPK 144

Query: 121 VQNFILWILAEIAIVACDIPEVIGTAFALNMLFSIPVWCGVLLTGLSTLFLLALQQYGIR 180
            QNFILW+LAEIAIVACDIPEVIGTAFALNMLF IPVWCGVLLTGLSTL LLALQQYGIR
Sbjct: 145 AQNFILWVLAEIAIVACDIPEVIGTAFALNMLFGIPVWCGVLLTGLSTLLLLALQQYGIR 204

Query: 181 KLEFLIAFLVLTIAICFFLELGYAKPDVGEIFYGLFVPQLKGSGATGLAISLLGAMVMPH 240
           KLEFLIAFLVLTIAICFFLELGYAKPDVGEIFYGLFVPQLKGSGATGLAISLLGAMVMPH
Sbjct: 205 KLEFLIAFLVLTIAICFFLELGYAKPDVGEIFYGLFVPQLKGSGATGLAISLLGAMVMPH 264

Query: 241 NLFLHSALVLSRKIPRSVSGIKEACRFYLIESGFALMVAFFINVSVISVSGAVCNSPDLN 300
           NLFLHSALVLSRKIPRS+SGIKEACRFY+IESGFALMVAF INVSVISVSGAVCN+PDLN
Sbjct: 265 NLFLHSALVLSRKIPRSLSGIKEACRFYMIESGFALMVAFLINVSVISVSGAVCNAPDLN 324

Query: 301 KEDQMSCNDLDLNKASFLLRNVLGKWSSKLFAIALLASGQSSTITGTYAGQYVMQGFLDL 360
           KEDQMSCNDLDLNKASFLLRNVLGKWSSKLFAIALLASGQSSTITGTYAGQYVMQGFLDL
Sbjct: 325 KEDQMSCNDLDLNKASFLLRNVLGKWSSKLFAIALLASGQSSTITGTYAGQYVMQGFLDL 384

Query: 361 KLTPWIRNFLTRSLAIVPSLIVAIIGGSSGAGQLIIIASMILSFELPFALIPLLKFTSSK 420
           KLTPWIRNFLTRSLAIVPSLIVAIIGGSSGAG+LIIIASMILSFELPFAL+PLLKFTSSK
Sbjct: 385 KLTPWIRNFLTRSLAIVPSLIVAIIGGSSGAGKLIIIASMILSFELPFALVPLLKFTSSK 444

Query: 421 TKMGPHVNSTAITVLTWIIGFLIMAINIYYLITRFIHVLLHNDLQLVAVVFIGILGFSGM 480
            KMGPHVNSTAITVLTWIIGFLIMAINIYYL++RFIHVLLHN+L L AVVFIGILGFSG+
Sbjct: 445 AKMGPHVNSTAITVLTWIIGFLIMAINIYYLMSRFIHVLLHNNLHLAAVVFIGILGFSGV 504

Query: 481 ALYLAGIAYLVLRKTKEITHFLALTTEESQRLSNEPSKTSGYNLPSEDIEIM 533
           ALYLAGIAYLVLRKTKEI+H LALTTEES+RLSN+PSKTSGY+LP+EDI  M
Sbjct: 505 ALYLAGIAYLVLRKTKEISHLLALTTEESRRLSNDPSKTSGYDLPNEDIVSM 556

BLAST of Lag0031241 vs. ExPASy TrEMBL
Match: A0A6J1DKN3 (metal transporter Nramp6 OS=Momordica charantia OX=3673 GN=LOC111020946 PE=3 SV=1)

HSP 1 Score: 959.5 bits (2479), Expect = 5.9e-276
Identity = 500/528 (94.70%), Postives = 514/528 (97.35%), Query Frame = 0

Query: 1   MAAGGSNSGQPQFLARAGDESFSHAPLIENPETDQIVVPDKKSWKNLFAYMGPGFLVSIA 60
           MAAGG +SGQPQFLARAGDESFSHAPLIEN ETDQIVVPDKKSWKNLFAYMGPGFLVSIA
Sbjct: 1   MAAGGPSSGQPQFLARAGDESFSHAPLIENSETDQIVVPDKKSWKNLFAYMGPGFLVSIA 60

Query: 61  YIDPGNFETDLQSGAQYKYGLLWIILVASCAALIIQSLAANLGVVTGKHLAEHCKSEYPK 120
           YIDPGNFETDLQSGAQYKYGLLWIILVASCAALIIQSLAANLGVVTGKHLAEHCK+EYP+
Sbjct: 61  YIDPGNFETDLQSGAQYKYGLLWIILVASCAALIIQSLAANLGVVTGKHLAEHCKAEYPR 120

Query: 121 VQNFILWILAEIAIVACDIPEVIGTAFALNMLFSIPVWCGVLLTGLSTLFLLALQQYGIR 180
           VQNFILW+LAEIAIVACDIPEVIGTAFALNMLFSIPVWCGVLLTGLSTL LLALQQYGIR
Sbjct: 121 VQNFILWVLAEIAIVACDIPEVIGTAFALNMLFSIPVWCGVLLTGLSTLLLLALQQYGIR 180

Query: 181 KLEFLIAFLVLTIAICFFLELGYAKPDVGEIFYGLFVPQLKGSGATGLAISLLGAMVMPH 240
           KLEFLIA LVLTIAICFFLELGYAKPD GEI YGLFVPQLKGSGATGLAISLLGAMVMPH
Sbjct: 181 KLEFLIAILVLTIAICFFLELGYAKPDAGEILYGLFVPQLKGSGATGLAISLLGAMVMPH 240

Query: 241 NLFLHSALVLSRKIPRSVSGIKEACRFYLIESGFALMVAFFINVSVISVSGAVCNSPDLN 300
           NLFLHSALVLSRKIPRSV+GIKEACRFY+IESGFALMVAF INVSVISVSGAVCN+P+LN
Sbjct: 241 NLFLHSALVLSRKIPRSVNGIKEACRFYMIESGFALMVAFLINVSVISVSGAVCNAPNLN 300

Query: 301 KEDQMSCNDLDLNKASFLLRNVLGKWSSKLFAIALLASGQSSTITGTYAGQYVMQGFLDL 360
           KEDQMSC+DLDLNKASFLLRNVLGKWSSKLFAIALLASGQSSTITGTYAGQYVMQGFLDL
Sbjct: 301 KEDQMSCSDLDLNKASFLLRNVLGKWSSKLFAIALLASGQSSTITGTYAGQYVMQGFLDL 360

Query: 361 KLTPWIRNFLTRSLAIVPSLIVAIIGGSSGAGQLIIIASMILSFELPFALIPLLKFTSSK 420
           KL PWIRNFLTRSLAIVPSLIVAIIGGSSGAG+LIIIASMILSFELPFAL+PLLKFTSSK
Sbjct: 361 KLRPWIRNFLTRSLAIVPSLIVAIIGGSSGAGKLIIIASMILSFELPFALVPLLKFTSSK 420

Query: 421 TKMGPHVNSTAITVLTWIIGFLIMAINIYYLITRFIHVLLHNDLQLVAVVFIGILGFSGM 480
           TKMGPHVNST ITVLTWIIGFLIMAINIYYL+TRFIHVLLHNDLQL AVVFIGILGFSGM
Sbjct: 421 TKMGPHVNSTVITVLTWIIGFLIMAINIYYLMTRFIHVLLHNDLQLAAVVFIGILGFSGM 480

Query: 481 ALYLAGIAYLVLRKTKEITHFLALTTEESQRLSNEPSKTSGYNLPSED 529
           ALYLAGIAYLV RKTKEIT+ LALTT ES RLSNEPSKTSGY+LPSED
Sbjct: 481 ALYLAGIAYLVFRKTKEITYLLALTTSESLRLSNEPSKTSGYSLPSED 528

BLAST of Lag0031241 vs. TAIR 10
Match: AT1G15960.1 (NRAMP metal ion transporter 6 )

HSP 1 Score: 778.9 bits (2010), Expect = 2.8e-225
Identity = 405/520 (77.88%), Postives = 458/520 (88.08%), Query Frame = 0

Query: 15  ARAGDESFSHAPLIENPETDQIVVPDKKSWKNLFAYMGPGFLVSIAYIDPGNFETDLQSG 74
           A   + S S++PLIEN +++QI+VP+KKSWKN F+Y+GPGFLVSIAYIDPGNFETDLQSG
Sbjct: 6   ASGSNRSISNSPLIENSDSNQILVPEKKSWKNFFSYLGPGFLVSIAYIDPGNFETDLQSG 65

Query: 75  AQYKYGLLWIILVASCAALIIQSLAANLGVVTGKHLAEHCKSEYPKVQNFILWILAEIAI 134
           AQYKY LLWIILVASCAAL+IQSLAANLGVVTGKHLAEHC++EY KV NF+LW++AEIA+
Sbjct: 66  AQYKYELLWIILVASCAALVIQSLAANLGVVTGKHLAEHCRAEYSKVPNFMLWVVAEIAV 125

Query: 135 VACDIPEVIGTAFALNMLFSIPVWCGVLLTGLSTLFLLALQQYGIRKLEFLIAFLVLTIA 194
           VACDIPEVIGTAFALNMLF+IPVW GVLLTGLSTL LLALQQYGIRKLEFLIAFLV TIA
Sbjct: 126 VACDIPEVIGTAFALNMLFNIPVWIGVLLTGLSTLILLALQQYGIRKLEFLIAFLVFTIA 185

Query: 195 ICFFLELGYAKPDVGEIFYGLFVPQLKGSGATGLAISLLGAMVMPHNLFLHSALVLSRKI 254
           +CFF+EL Y+KPD  E+ YGLFVPQLKG+GATGLAISLLGAMVMPHNLFLHSALVLSRKI
Sbjct: 186 LCFFVELHYSKPDPKEVLYGLFVPQLKGNGATGLAISLLGAMVMPHNLFLHSALVLSRKI 245

Query: 255 PRSVSGIKEACRFYLIESGFALMVAFFINVSVISVSGAVCNSPDLNKEDQMSCNDLDLNK 314
           PRSV+GIKEACR+YLIESG ALMVAF INVSVISVSGAVCN+ DL+ ED+ SC DLDLNK
Sbjct: 246 PRSVTGIKEACRYYLIESGLALMVAFLINVSVISVSGAVCNASDLSPEDRASCQDLDLNK 305

Query: 315 ASFLLRNVLGKWSSKLFAIALLASGQSSTITGTYAGQYVMQGFLDLKLTPWIRNFLTRSL 374
           ASFLLRNV+GKWSSKLFAIALLASGQSSTITGTYAGQYVMQGFLDL+L PW+RNFLTR L
Sbjct: 306 ASFLLRNVVGKWSSKLFAIALLASGQSSTITGTYAGQYVMQGFLDLRLEPWLRNFLTRCL 365

Query: 375 AIVPSLIVAIIGGSSGAGQLIIIASMILSFELPFALIPLLKFTSSKTKMGPHVNSTAITV 434
           AI+PSLIVA+IGGS+GAG+LIIIASMILSFELPFAL+PLLKFTSSKTKMG H NS  I+ 
Sbjct: 366 AIIPSLIVALIGGSAGAGKLIIIASMILSFELPFALVPLLKFTSSKTKMGSHANSLVISS 425

Query: 435 LTWIIGFLIMAINIYYLITRFIHVLLHNDLQLVAVVFIGILGFSGMALYLAGIAYLVLRK 494
           +TWIIG LIM INIYYL++ FI +LLH+ + LVA+VF+G+LGFSG+A YLA I+YLVLRK
Sbjct: 426 VTWIIGGLIMGINIYYLVSSFIKLLLHSHMNLVAIVFLGVLGFSGIATYLAAISYLVLRK 485

Query: 495 TKE--ITHFLALTTEESQRLSNEPSKTSGYNLPSEDIEIM 533
            +E   THFL  +  +++             LP EDI  M
Sbjct: 486 NRESSSTHFLDFSNSQTEE-----------TLPREDIANM 514

BLAST of Lag0031241 vs. TAIR 10
Match: AT1G80830.1 (natural resistance-associated macrophage protein 1 )

HSP 1 Score: 774.2 bits (1998), Expect = 6.8e-224
Identity = 404/517 (78.14%), Postives = 460/517 (88.97%), Query Frame = 0

Query: 1   MAAGGSNSGQPQFLARA-GDESFSHAPLIENPETDQIVVPDKKSWKNLFAYMGPGFLVSI 60
           MAA G  SG+ QF++ + G+ SFS++PLIEN +++QI+V +KKSWKN FAY+GPGFLVSI
Sbjct: 1   MAATG--SGRSQFISSSGGNRSFSNSPLIENSDSNQIIVSEKKSWKNFFAYLGPGFLVSI 60

Query: 61  AYIDPGNFETDLQSGAQYKYGLLWIILVASCAALIIQSLAANLGVVTGKHLAEHCKSEYP 120
           AYIDPGNFETDLQ+GA YKY LLWIILVASCAAL+IQSLAANLGVVTGKHLAE C++EY 
Sbjct: 61  AYIDPGNFETDLQAGAHYKYELLWIILVASCAALVIQSLAANLGVVTGKHLAEQCRAEYS 120

Query: 121 KVQNFILWILAEIAIVACDIPEVIGTAFALNMLFSIPVWCGVLLTGLSTLFLLALQQYGI 180
           KV NF+LW++AEIA+VACDIPEVIGTAFALNMLFSIPVW GVLLTGLSTL LLALQ+YG+
Sbjct: 121 KVPNFMLWVVAEIAVVACDIPEVIGTAFALNMLFSIPVWIGVLLTGLSTLILLALQKYGV 180

Query: 181 RKLEFLIAFLVLTIAICFFLELGYAKPDVGEIFYGLFVPQLKGSGATGLAISLLGAMVMP 240
           RKLEFLIAFLV TIAICFF+EL Y+KPD GE+ +GLFVPQLKG+GATGLAISLLGAMVMP
Sbjct: 181 RKLEFLIAFLVFTIAICFFVELHYSKPDPGEVLHGLFVPQLKGNGATGLAISLLGAMVMP 240

Query: 241 HNLFLHSALVLSRKIPRSVSGIKEACRFYLIESGFALMVAFFINVSVISVSGAVCNSPDL 300
           HNLFLHSALVLSRKIPRS SGIKEACRFYLIESG ALMVAF INVSVISVSGAVCN+P+L
Sbjct: 241 HNLFLHSALVLSRKIPRSASGIKEACRFYLIESGLALMVAFLINVSVISVSGAVCNAPNL 300

Query: 301 NKEDQMSCNDLDLNKASFLLRNVLGKWSSKLFAIALLASGQSSTITGTYAGQYVMQGFLD 360
           + ED+ +C DLDLNKASFLLRNV+GKWSSKLFAIALLASGQSSTITGTYAGQYVMQGFLD
Sbjct: 301 SPEDRANCEDLDLNKASFLLRNVVGKWSSKLFAIALLASGQSSTITGTYAGQYVMQGFLD 360

Query: 361 LKLTPWIRNFLTRSLAIVPSLIVAIIGGSSGAGQLIIIASMILSFELPFALIPLLKFTSS 420
           L+L PW+RN LTR LAI+PSLIVA+IGGS+GAG+LIIIASMILSFELPFAL+PLLKFTS 
Sbjct: 361 LRLEPWLRNLLTRCLAIIPSLIVALIGGSAGAGKLIIIASMILSFELPFALVPLLKFTSC 420

Query: 421 KTKMGPHVNSTAITVLTWIIGFLIMAINIYYLITRFIHVLLHNDLQLVAVVFIGILGFSG 480
           KTKMG HVN  AIT LTW+IG LIM INIYYL++ FI +L+H+ ++L+ VVF GILGF+G
Sbjct: 421 KTKMGSHVNPMAITALTWVIGGLIMGINIYYLVSSFIKLLIHSHMKLILVVFCGILGFAG 480

Query: 481 MALYLAGIAYLVLRKTKEITHFLALTTEESQRLSNEP 517
           +ALYLA IAYLV RK +  T    L + +SQ +   P
Sbjct: 481 IALYLAAIAYLVFRKNRVATSL--LISRDSQNVETLP 513

BLAST of Lag0031241 vs. TAIR 10
Match: AT5G67330.1 (natural resistance associated macrophage protein 4 )

HSP 1 Score: 313.5 bits (802), Expect = 3.3e-85
Identity = 192/461 (41.65%), Postives = 284/461 (61.61%), Query Frame = 0

Query: 41  KKSWKNLFAYMGPGFLVSIAYIDPGNFETDLQSGAQYKYGLLWIILVASCAALIIQSLAA 100
           K SWK L+ + GPGFL+SIA++DPGN E+DLQ+GA   Y L+W+++ A+   L+IQ L+A
Sbjct: 46  KFSWKKLWLFTGPGFLMSIAFLDPGNLESDLQAGAIAGYSLIWLLMWATAIGLLIQLLSA 105

Query: 101 NLGVVTGKHLAEHCKSEYPKVQNFILWILAEIAIVACDIPEVIGTAFALNMLFS--IPVW 160
            LGV TG+HLAE C+ EYP     +LWI+AEIA++  DI EVIG+A A+ +L +  +P+W
Sbjct: 106 RLGVATGRHLAELCREEYPTWARMVLWIMAEIALIGADIQEVIGSAIAIKILSNGLVPLW 165

Query: 161 CGVLLTGLSTLFLLALQQYGIRKLEFLIAFLVLTIAICFFLELGYAKPDVGEIFYGLFVP 220
            GV++T L     L L+ YGIRKLE + A L+ T+A+ F    G  KP   E+  G  VP
Sbjct: 166 AGVVITALDCFIFLFLENYGIRKLEAVFAILIATMALAFAWMFGQTKPSGTELLVGALVP 225

Query: 221 QLKGSGATGLAISLLGAMVMPHNLFLHSALVLSRKI-PRSVSGIKEACRFYLIESGFALM 280
           +L  S     A+ ++G ++MPHN+FLHSALV SR++ P+    +KEA ++Y IES  AL 
Sbjct: 226 KL-SSRTIKQAVGIVGCIIMPHNVFLHSALVQSREVDPKKRFRVKEALKYYSIESTGALA 285

Query: 281 VAFFINVSVISVSGAVCNSPDLNKEDQMSCNDLDLNKASFLLRNVLGKWSSK---LFAIA 340
           V+F INV V +V        ++        + + L  A   L++  G        ++AI 
Sbjct: 286 VSFIINVFVTTVFAKSFYGTEI-------ADTIGLANAGQYLQDKYGGGFFPILYIWAIG 345

Query: 341 LLASGQSSTITGTYAGQYVMQGFLDLKLTPWIRNFLTRSLAIVPSLIVAIIGGSSGA--G 400
           +LA+GQSSTITGTYAGQ++M GFL+LK+  W+R  +TRS AI+P++IVA++  SS +   
Sbjct: 346 VLAAGQSSTITGTYAGQFIMGGFLNLKMKKWVRALITRSCAIIPTMIVALVFDSSDSMLD 405

Query: 401 QLIIIASMILSFELPFALIPLLKFTSSKTKMGPHVNSTAITVLTWIIGFLIMAINIYYLI 460
           +L    +++ S ++PFA+IPLL   S++  MG       +  ++WI+  L++AIN  YL+
Sbjct: 406 ELNEWLNVLQSVQIPFAVIPLLCLVSNEQIMGSFKIQPLVQTISWIVAALVIAIN-GYLM 465

Query: 461 TRFIHVLLHNDLQLVAVVFIGILGFSGMALYLAGIAYLVLR 494
             F      N + LV V+   I        Y+  + YL+ R
Sbjct: 466 VDFFSGAATNLILLVPVIIFAI-------AYVVFVLYLISR 490

BLAST of Lag0031241 vs. TAIR 10
Match: AT4G18790.1 (NRAMP metal ion transporter family protein )

HSP 1 Score: 310.5 bits (794), Expect = 2.8e-84
Identity = 189/475 (39.79%), Postives = 285/475 (60.00%), Query Frame = 0

Query: 43  SWKNLFAYMGPGFLVSIAYIDPGNFETDLQSGAQYKYGLLWIILVASCAALIIQSLAANL 102
           SW  L+ + GPGFL+SIA++DPGN E DLQ+GA   Y LLW++L A+   L++Q L+A +
Sbjct: 66  SWAKLWKFTGPGFLMSIAFLDPGNIEGDLQAGAVAGYSLLWLLLWATLMGLLMQLLSARI 125

Query: 103 GVVTGKHLAEHCKSEYPKVQNFILWILAEIAIVACDIPEVIGTAFALNMLFS--IPVWCG 162
           GV TG+HLAE C+SEYP     +LW +AE+A++  DI EVIG+A AL +L    +P+W G
Sbjct: 126 GVATGRHLAEICRSEYPSWARILLWFMAEVALIGADIQEVIGSAIALQILTRGFLPIWVG 185

Query: 163 VLLTGLSTLFLLALQQYGIRKLEFLIAFLVLTIAICFFLELGYAKPDVGEIFYGLFVPQL 222
           V++T      +  L++ G+RKLE L A L+ T+A+ F       KP V E+F G+ +P+L
Sbjct: 186 VIITSFDCFLISYLEKCGMRKLEGLFAVLIATMALSFAWMFNETKPSVEELFIGIIIPKL 245

Query: 223 KGSGATGLAISLLGAMVMPHNLFLHSALVLSRKI-PRSVSGIKEACRFYLIESGFALMVA 282
            GS     A+ ++G ++ PHN+FLHSALV SRK  P+ ++ ++EA  +Y IES  AL V+
Sbjct: 246 -GSKTIREAVGVVGCVITPHNVFLHSALVQSRKTDPKEINRVQEALNYYTIESSAALFVS 305

Query: 283 FFINVSVISVSGAVCNSPDLNKEDQMSCNDLDLNKASFLLRNVLGKWSSK---LFAIALL 342
           F IN+ V +V                  + + L  A + L+   G        ++ I LL
Sbjct: 306 FMINLFVTAVFAK-------GFYGTKQADSIGLVNAGYYLQEKYGGGVFPILYIWGIGLL 365

Query: 343 ASGQSSTITGTYAGQYVMQGFLDLKLTPWIRNFLTRSLAIVPSLIVAIIGGSSGAGQLII 402
           A+GQSSTITGTYAGQ++M+GFLDL++  W+  F+TRS AIVP++ VAI+  +S  G L +
Sbjct: 366 AAGQSSTITGTYAGQFIMEGFLDLQMEQWLSAFITRSFAIVPTMFVAIMFNTS-EGSLDV 425

Query: 403 I---ASMILSFELPFALIPLLKFTSSKTKMGPHVNSTAITVLTWIIGFLIMAINIYYLIT 462
           +    +++ S ++PFA+IPLL   S++  MG      ++  L W +   +M IN Y L+ 
Sbjct: 426 LNEWLNILQSMQIPFAVIPLLTMVSNEHIMGVFKIGPSLEKLAWTVAVFVMMINGYLLLD 485

Query: 463 RFIHVLLHNDLQLVAVVFIGILGFSGMALYLAGIAYLVLRKTKEITHFLALTTEE 509
            F        +  V    +G L F G+  Y++ I YLV  ++ + + + +L   E
Sbjct: 486 FF--------MAEVEGFLVGFLVFGGVVGYISFIIYLVSYRSSQSSSWSSLEMSE 523

BLAST of Lag0031241 vs. TAIR 10
Match: AT2G23150.1 (natural resistance-associated macrophage protein 3 )

HSP 1 Score: 307.0 bits (785), Expect = 3.1e-83
Identity = 188/459 (40.96%), Postives = 278/459 (60.57%), Query Frame = 0

Query: 43  SWKNLFAYMGPGFLVSIAYIDPGNFETDLQSGAQYKYGLLWIILVASCAALIIQSLAANL 102
           SWK L+ + GPGFL+SIA++DPGN E DLQ+GA   Y LLW+++ A+   L++Q L+A L
Sbjct: 52  SWKKLWLFTGPGFLMSIAFLDPGNLEGDLQAGAVAGYSLLWLLMWATAMGLLVQLLSARL 111

Query: 103 GVVTGKHLAEHCKSEYPKVQNFILWILAEIAIVACDIPEVIGTAFALNMLFS--IPVWCG 162
           GV TG+HLAE C+ EYP     +LW++AE+A++  DI EVIG+A A+ +L +  +P+W G
Sbjct: 112 GVATGRHLAELCRDEYPTWARMVLWVMAELALIGSDIQEVIGSAIAIKILSNGILPLWAG 171

Query: 163 VLLTGLSTLFLLALQQYGIRKLEFLIAFLVLTIAICFFLELGYAKPDVGEIFYGLFVPQL 222
           V++T L     L L+ YGIRKLE + A L+ T+ + F    G AKP   E+  G+ VP+L
Sbjct: 172 VVITALDCFVFLFLENYGIRKLEAVFAVLIATMGVSFAWMFGQAKPSGSELLIGILVPKL 231

Query: 223 KGSGATGLAISLLGAMVMPHNLFLHSALVLSRKI-PRSVSGIKEACRFYLIESGFALMVA 282
             S     A+ ++G ++MPHN+FLHSALV SR++  R    ++EA  +Y IES  AL ++
Sbjct: 232 -SSRTIQKAVGVVGCIIMPHNVFLHSALVQSREVDKRQKYRVQEALNYYTIESTIALFIS 291

Query: 283 FFINVSVISVSGAVCNSPDLNKEDQMSCNDLDLNKASFLLRNVLGKWSSK---LFAIALL 342
           F IN+ V +V      + DL        N + L  A   L+   G        ++AI LL
Sbjct: 292 FLINLFVTTVFAKGFYNTDL-------ANSIGLVNAGQYLQEKYGGGVFPILYIWAIGLL 351

Query: 343 ASGQSSTITGTYAGQYVMQGFLDLKLTPWIRNFLTRSLAIVPSLIVAIIGGSSGAGQLII 402
           A+GQSSTITGTYAGQ++M GFL+ K+  W+R  +TRS AI+P++IVA++  SS A   ++
Sbjct: 352 AAGQSSTITGTYAGQFIMGGFLNFKMKKWLRALITRSCAIIPTIIVALVFDSSEATLDVL 411

Query: 403 --IASMILSFELPFALIPLLKFTSSKTKMGPHVNSTAITVLTWIIGFLIMAINIYYLITR 462
               +++ S ++PFALIPLL   S +  MG          + W++  L++ IN Y L+  
Sbjct: 412 NEWLNVLQSIQIPFALIPLLCLVSKEQIMGSFKIGPLYKTIAWLVAALVIMINGYLLLEF 471

Query: 463 FIHVLLHNDLQLVAVVFIGILGFSGMALYLAGIAYLVLR 494
           F +       ++  +V+ G +     A Y A I YL+ R
Sbjct: 472 FSN-------EVSGIVYTGFVTLF-TASYGAFILYLIAR 494

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038905496.11.6e-28095.49metal transporter Nramp6-like [Benincasa hispida] >XP_038905497.1 metal transpor... [more]
XP_022982780.16.9e-27995.09metal transporter Nramp6-like [Cucurbita maxima][more]
KAG6581627.13.4e-27894.55Metal transporter Nramp1, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022935446.14.5e-27894.36metal transporter Nramp6-like [Cucurbita moschata][more]
XP_023528489.13.8e-27794.36metal transporter Nramp6-like [Cucurbita pepo subsp. pepo] >XP_023528490.1 metal... [more]
Match NameE-valueIdentityDescription
Q9S9N83.9e-22477.88Metal transporter Nramp6 OS=Arabidopsis thaliana OX=3702 GN=NRAMP6 PE=2 SV=2[more]
Q9SAH89.5e-22378.14Metal transporter Nramp1 OS=Arabidopsis thaliana OX=3702 GN=NRAMP1 PE=1 SV=1[more]
Q653V61.4e-21372.54Metal transporter Nramp3 OS=Oryza sativa subsp. japonica OX=39947 GN=NRAMP3 PE=2... [more]
Q8H4H55.9e-16461.80Metal transporter Nramp5 OS=Oryza sativa subsp. japonica OX=39947 GN=NRAMP5 PE=2... [more]
Q0D7E42.8e-15862.83Metal transporter Nramp1 OS=Oryza sativa subsp. japonica OX=39947 GN=NRAMP1 PE=2... [more]
Match NameE-valueIdentityDescription
A0A6J1J3T23.3e-27995.09metal transporter Nramp6-like OS=Cucurbita maxima OX=3661 GN=LOC111481537 PE=3 S... [more]
A0A6J1F4P32.2e-27894.36metal transporter Nramp6-like OS=Cucurbita moschata OX=3662 GN=LOC111442317 PE=3... [more]
A0A5A7T2K93.1e-27793.98Metal transporter Nramp6 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffol... [more]
A0A5D3DUP03.1e-27793.98Metal transporter Nramp6 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffol... [more]
A0A6J1DKN35.9e-27694.70metal transporter Nramp6 OS=Momordica charantia OX=3673 GN=LOC111020946 PE=3 SV=... [more]
Match NameE-valueIdentityDescription
AT1G15960.12.8e-22577.88NRAMP metal ion transporter 6 [more]
AT1G80830.16.8e-22478.14natural resistance-associated macrophage protein 1 [more]
AT5G67330.13.3e-8541.65natural resistance associated macrophage protein 4 [more]
AT4G18790.12.8e-8439.79NRAMP metal ion transporter family protein [more]
AT2G23150.13.1e-8340.96natural resistance-associated macrophage protein 3 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001046NRAMP familyPRINTSPR00447NATRESASSCMPcoord: 339..358
score: 77.8
coord: 126..152
score: 56.74
coord: 154..173
score: 43.8
coord: 229..252
score: 63.0
coord: 179..200
score: 42.18
coord: 399..418
score: 53.4
coord: 367..384
score: 53.78
IPR001046NRAMP familyPFAMPF01566Nrampcoord: 67..429
e-value: 1.9E-122
score: 408.7
IPR001046NRAMP familyTIGRFAMTIGR01197TIGR01197coord: 46..423
e-value: 9.4E-124
score: 411.6
IPR001046NRAMP familyPANTHERPTHR11706SOLUTE CARRIER PROTEIN FAMILY 11 MEMBERcoord: 8..507
IPR001046NRAMP familyHAMAPMF_00221NRAMPcoord: 32..455
score: 39.340843
NoneNo IPR availablePANTHERPTHR11706:SF85METAL TRANSPORTER NRAMP1coord: 8..507

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0031241.1Lag0031241.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0070574 cadmium ion transmembrane transport
biological_process GO:0071421 manganese ion transmembrane transport
biological_process GO:0030001 metal ion transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane
molecular_function GO:0015086 cadmium ion transmembrane transporter activity
molecular_function GO:0005384 manganese ion transmembrane transporter activity
molecular_function GO:0046873 metal ion transmembrane transporter activity