Lag0031227 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0031227
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	Phytocyanin domain-containing protein
Location	chr11: 5975846 .. 5976863 (-)
RNA-Seq Expression	Lag0031227
Synteny	Lag0031227

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCCGCCCTCCCCCCAGCTCATCGCCGTCTTCCTCCTCCTCCTCTGCGCCGCCGTCTTCCCCGTCTCCGCCACCGACCACATCGTCGGCGCCAACCGCGGCTGGAACCCCGGCATCAACTACACTCTCTGGGCTAACAACCACACCTTCTTCGTTGGCGACTTCATCTGTAAGTTGGAACTTCTCCCTCTCTCTCCCCCCCTGAAATGTTGTGTAATGTTTGATTCTAGAAATTGAATCAAATTAGAGGAAAAGTTTCAGAATCTTAAATTGTTTTGTATTCAAGCTAATCTGAGCATGATTTGAGTGTTGAAACGTACCAAAAATTCGAAAGTCAAAAGTTCGAATTCCCACCTCATAATTATAATGTTGTTCACATTTGTTGGCTTCTTCCTTTTCTACTCTACTCTGAGTTAGAGTATTATTAAGGTTATACTAGATTTGGATATACTAGTAGTGAAATAATTGTAATCGTCTTTCAATGGCTTGTTGTTACGATAGGATTGGTTATTAATAGAGAGAATCAGAGAATACGGAGGCTAGGCATATTAGAAGCCATTCGAGGGTAGATTCGCAAGAGAGATAGCTCTCTTAATAGGGTCATTGTTATATTGTAATTTCTCTTTTTTACATTACGATTGAGATCGTGACTCGTAGCTTCGGAACCAAATATTTGGCAGCATTTAGGTATCAAAAGAGCCAATACAATGTGTTTGAGGTGAACCAATCAGGCTACGACAACTGCACCACAGAAGGGGCGCTTGGGAATTGGAGCAGTGGCAAGGACTTCATCCTCCTCAACAACTCCCAAAGGTACTACTTCATTTGTGGCAATGGGCAGTGTTTCAATGGCATGAAGGTGTCCATTCTTGTTCATCCTCTTCCTCCTGCTCCATCTTCTAGTCCCAATTCCACCTCGCCCTCCTCCGCCACAGCTCGAGCGGGTTCTCGACAATGGCGGATCCGGGCTCTGCTCATCTCTCTGGCTTTGGTTTGGTTTGGTTTGGTTTGGATCTGA

mRNA sequence

ATGCCGCCCTCCCCCCAGCTCATCGCCGTCTTCCTCCTCCTCCTCTGCGCCGCCGTCTTCCCCGTCTCCGCCACCGACCACATCGTCGGCGCCAACCGCGGCTGGAACCCCGGCATCAACTACACTCTCTGGGCTAACAACCACACCTTCTTCGTTGGCGACTTCATCTCATTTAGGTATCAAAAGAGCCAATACAATGTGTTTGAGGTGAACCAATCAGGCTACGACAACTGCACCACAGAAGGGGCGCTTGGGAATTGGAGCAGTGGCAAGGACTTCATCCTCCTCAACAACTCCCAAAGGTACTACTTCATTTGTGGCAATGGGCAGTGTTTCAATGGCATGAAGGTGTCCATTCTTGTTCATCCTCTTCCTCCTGCTCCATCTTCTAGTCCCAATTCCACCTCGCCCTCCTCCGCCACAGCTCGAGCGGGTTCTCGACAATGGCGGATCCGGGCTCTGCTCATCTCTCTGGCTTTGGTTTGGTTTGGTTTGGTTTGGATCTGA

Coding sequence (CDS)

ATGCCGCCCTCCCCCCAGCTCATCGCCGTCTTCCTCCTCCTCCTCTGCGCCGCCGTCTTCCCCGTCTCCGCCACCGACCACATCGTCGGCGCCAACCGCGGCTGGAACCCCGGCATCAACTACACTCTCTGGGCTAACAACCACACCTTCTTCGTTGGCGACTTCATCTCATTTAGGTATCAAAAGAGCCAATACAATGTGTTTGAGGTGAACCAATCAGGCTACGACAACTGCACCACAGAAGGGGCGCTTGGGAATTGGAGCAGTGGCAAGGACTTCATCCTCCTCAACAACTCCCAAAGGTACTACTTCATTTGTGGCAATGGGCAGTGTTTCAATGGCATGAAGGTGTCCATTCTTGTTCATCCTCTTCCTCCTGCTCCATCTTCTAGTCCCAATTCCACCTCGCCCTCCTCCGCCACAGCTCGAGCGGGTTCTCGACAATGGCGGATCCGGGCTCTGCTCATCTCTCTGGCTTTGGTTTGGTTTGGTTTGGTTTGGATCTGA

Protein sequence

MPPSPQLIAVFLLLLCAAVFPVSATDHIVGANRGWNPGINYTLWANNHTFFVGDFISFRYQKSQYNVFEVNQSGYDNCTTEGALGNWSSGKDFILLNNSQRYYFICGNGQCFNGMKVSILVHPLPPAPSSSPNSTSPSSATARAGSRQWRIRALLISLALVWFGLVWI

Homology

[Close]

BLAST of Lag0031227 vs. NCBI nr
Match: KAG7018121.1 (Lamin-like protein [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 291.2 bits (744), Expect = 5.7e-75
Identity = 143/171 (83.63%), Postives = 152/171 (88.89%), Query Frame = 0


Query: 1   MPPSPQLIAVFLLLLCAA-VFPVSATDHIVGANRGWNPGINYTLWANNHTFFVGDFISFR 60
           MP SPQL+A FLLL  AA VFPVSATDHIVGANRGWNPGINYTLWANNHTFFVGD ISFR
Sbjct: 1   MPASPQLLAFFLLLCTAASVFPVSATDHIVGANRGWNPGINYTLWANNHTFFVGDLISFR 60

Query: 61  YQKSQYNVFEVNQSGYDNCTTEGALGNWSSGKDFILLNNSQRYYFICGNGQCFNGMKVSI 120
           YQK+QYNVFEVNQSGYDNCTTEGA GNWSSGKDFI LN +QRYYFICGNGQCFNGMKV+I
Sbjct: 61  YQKNQYNVFEVNQSGYDNCTTEGAFGNWSSGKDFIPLNKAQRYYFICGNGQCFNGMKVTI 120

Query: 121 LVHPLPPAPSS--SPNSTSPSSATARAGSRQWRIRALLISLALVWFGLVWI 169
           LVHPLPP PSS  +   +SPSSAT R GSRQW+IRALL+SLAL+WFG  WI
Sbjct: 121 LVHPLPPPPSSPLASKDSSPSSATPRTGSRQWKIRALLVSLALIWFGSDWI 171

[Close]

BLAST of Lag0031227 vs. NCBI nr
Match: XP_022934432.1 (lamin-like protein [Cucurbita moschata])

HSP 1 Score: 289.3 bits (739), Expect = 2.2e-74
Identity = 142/171 (83.04%), Postives = 151/171 (88.30%), Query Frame = 0


Query: 1   MPPSPQLIAVFLLLLCAA-VFPVSATDHIVGANRGWNPGINYTLWANNHTFFVGDFISFR 60
           MP SPQL+A FLLL  AA VFPVSATDHIVGANRGWNPGINYTLWANNHTFFVGD ISFR
Sbjct: 1   MPASPQLLAFFLLLCTAASVFPVSATDHIVGANRGWNPGINYTLWANNHTFFVGDLISFR 60

Query: 61  YQKSQYNVFEVNQSGYDNCTTEGALGNWSSGKDFILLNNSQRYYFICGNGQCFNGMKVSI 120
           YQK+QYNVFEVNQSGYDNCTTEGA GNWSSGKDFI LN +QRYYFICGNGQCFNGMKV+I
Sbjct: 61  YQKNQYNVFEVNQSGYDNCTTEGAFGNWSSGKDFIPLNKAQRYYFICGNGQCFNGMKVTI 120

Query: 121 LVHPLPPAPSS--SPNSTSPSSATARAGSRQWRIRALLISLALVWFGLVWI 169
           LVHPLPP PSS  +   +SPSSAT R GSRQW+IRAL +SLAL+WFG  WI
Sbjct: 121 LVHPLPPPPSSPLASKDSSPSSATPRTGSRQWQIRALFVSLALIWFGSDWI 171

[Close]

BLAST of Lag0031227 vs. NCBI nr
Match: XP_023526524.1 (lamin-like protein [Cucurbita pepo subsp. pepo])

HSP 1 Score: 288.9 bits (738), Expect = 2.8e-74
Identity = 142/171 (83.04%), Postives = 151/171 (88.30%), Query Frame = 0


Query: 1   MPPSPQLIAVFLLLLCAA-VFPVSATDHIVGANRGWNPGINYTLWANNHTFFVGDFISFR 60
           MP SP L+A FLLL  AA VFPVSATDHIVGANRGWNPGINYTLWANNHTFFVGD ISFR
Sbjct: 1   MPASPHLLAFFLLLCTAASVFPVSATDHIVGANRGWNPGINYTLWANNHTFFVGDLISFR 60

Query: 61  YQKSQYNVFEVNQSGYDNCTTEGALGNWSSGKDFILLNNSQRYYFICGNGQCFNGMKVSI 120
           YQK+QYNVFEVNQSGYDNCTTEGA GNWSSGKDFI LN +QRYYFICGNGQCFNGMKV+I
Sbjct: 61  YQKNQYNVFEVNQSGYDNCTTEGAFGNWSSGKDFIPLNKAQRYYFICGNGQCFNGMKVTI 120

Query: 121 LVHPLPPAPSS--SPNSTSPSSATARAGSRQWRIRALLISLALVWFGLVWI 169
           LVHPLPP PSS  +   +SPSSAT R GSRQW+IRALL+SLAL+WFG  WI
Sbjct: 121 LVHPLPPPPSSPLASKDSSPSSATPRTGSRQWQIRALLVSLALIWFGSDWI 171

[Close]

BLAST of Lag0031227 vs. NCBI nr
Match: KAG6581622.1 (Lamin-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 287.7 bits (735), Expect = 6.3e-74
Identity = 142/171 (83.04%), Postives = 151/171 (88.30%), Query Frame = 0


Query: 1   MPPSPQLIAVFLLLLCAA-VFPVSATDHIVGANRGWNPGINYTLWANNHTFFVGDFISFR 60
           MP SPQL+A FLLL  AA VFPVSATDHIVGANRGWNPGINYTLWANNHTFFVGD ISFR
Sbjct: 1   MPASPQLLAFFLLLCTAASVFPVSATDHIVGANRGWNPGINYTLWANNHTFFVGDLISFR 60

Query: 61  YQKSQYNVFEVNQSGYDNCTTEGALGNWSSGKDFILLNNSQRYYFICGNGQCFNGMKVSI 120
           YQK+QYNVFEVNQSGYDNCTTEGA GNWSSGKDFI LN +QRYYFICGNGQCFNGMKV+I
Sbjct: 61  YQKNQYNVFEVNQSGYDNCTTEGAFGNWSSGKDFIPLNKAQRYYFICGNGQCFNGMKVTI 120

Query: 121 LVHPLPPAPSS--SPNSTSPSSATARAGSRQWRIRALLISLALVWFGLVWI 169
           LVHPLPP PSS  +   +SPSSAT   GSRQW+IRALL+SLAL+WFG  WI
Sbjct: 121 LVHPLPPPPSSPLASKDSSPSSATPWTGSRQWQIRALLVSLALIWFGSDWI 171

[Close]

BLAST of Lag0031227 vs. NCBI nr
Match: XP_022983448.1 (lamin-like protein [Cucurbita maxima])

HSP 1 Score: 285.4 bits (729), Expect = 3.1e-73
Identity = 141/171 (82.46%), Postives = 151/171 (88.30%), Query Frame = 0


Query: 1   MPPSPQLIAVFLLLLCAA-VFPVSATDHIVGANRGWNPGINYTLWANNHTFFVGDFISFR 60
           MP S QL+  FLLL  AA VFPVSATDHIVGANRGWNPGINYTLWANNHTFFVGD ISFR
Sbjct: 1   MPASLQLLPFFLLLCTAASVFPVSATDHIVGANRGWNPGINYTLWANNHTFFVGDLISFR 60

Query: 61  YQKSQYNVFEVNQSGYDNCTTEGALGNWSSGKDFILLNNSQRYYFICGNGQCFNGMKVSI 120
           YQK+QYNVFEVNQSGYDNCTTEGA GNWSSGKDFI LN +QRYYFICGNGQCFNGMKV+I
Sbjct: 61  YQKNQYNVFEVNQSGYDNCTTEGAFGNWSSGKDFIPLNKAQRYYFICGNGQCFNGMKVTI 120

Query: 121 LVHPLPPAPSS--SPNSTSPSSATARAGSRQWRIRALLISLALVWFGLVWI 169
           LVHPLPP PSS  +  ++SPSSAT R GSRQW+IRALL+SLAL+WFG  WI
Sbjct: 121 LVHPLPPPPSSPLASKNSSPSSATPRTGSRQWQIRALLVSLALIWFGSDWI 171

[Close]

BLAST of Lag0031227 vs. ExPASy Swiss-Prot
Match: Q39131 (Lamin-like protein OS=Arabidopsis thaliana OX=3702 GN=At5g15350 PE=2 SV=1)

HSP 1 Score: 119.8 bits (299), Expect = 3.0e-26
Identity = 63/161 (39.13%), Postives = 96/161 (59.63%), Query Frame = 0


Query: 7   LIAVFLLLLCAAVFP-VSATDHIVGANRGWNPGINYTLWANNHTFFVGDFISFRYQKSQY 66
           + AV L  L AA  P V+A  + VG N+ WNP INYT+WA    F++GD++ F + ++Q+
Sbjct: 8   ITAVVLAFLMAAPMPGVTAKKYTVGENKFWNPNINYTIWAQGKHFYLGDWLYFVFDRNQH 67

Query: 67  NVFEVNQSGYDNCTTEGALGNWS--SGKDFILLNNSQRYYFICGNGQCFNGMKVSILVHP 126
           N+ EVN++ Y+ C  +  + NW+  +G+D + LN ++ YY + G G C+ GMK+S+ V  
Sbjct: 68  NILEVNKTDYEGCIADHPIRNWTRGAGRDIVTLNQTKHYYLLDGKGGCYGGMKLSVKVEK 127

Query: 127 LPPAPSSSP--NSTSPSSATARAGSRQWRIRALLISLALVW 163
           LPP P S+P  N  S S  T   G  Q+ I   L +   +W
Sbjct: 128 LPPPPKSAPVKNIGSVSMVT---GLAQFMIPVSLFAFPAMW 165

[Close]

BLAST of Lag0031227 vs. ExPASy Swiss-Prot
Match: A0A072U307 (Blue copper protein 1b OS=Medicago truncatula OX=3880 GN=BCP1B PE=2 SV=1)

HSP 1 Score: 83.2 bits (204), Expect = 3.1e-15
Identity = 48/159 (30.19%), Postives = 92/159 (57.86%), Query Frame = 0


Query: 7   LIAVFLLLLCAAVFPVSATDHIVGANRGWNPGINYTLWANNHTFFVGDFISFRYQKSQYN 66
           ++++ ++LL +    ++ATD+IVG ++GW    +YT WA +  F VGD + F Y  S++N
Sbjct: 9   ILSISMVLLSSVA--IAATDYIVGDDKGWTVDFDYTQWAQDKVFRVGDNLVFNYDPSRHN 68

Query: 67  VFEVNQSGYDNCTTEGALGNWSSGKDFILLNNSQRYYFICGNGQCFNGMKVSILVHPL-- 126
           VF+VN + + +CT        S+GKD I L    R +++CG     +  ++ +++  L  
Sbjct: 69  VFKVNGTLFQSCTFPPKNEALSTGKDIIQLKTEGRKWYVCGVADHCSARQMKLVITVLAE 128

Query: 127 -PPAPSSSPNSTSPSSATARAGSRQWRIRALLISLALVW 163
             PAPS  P+S + S  ++  G     + A+++++A+++
Sbjct: 129 GAPAPSPPPSSDAHSVVSSLFGV----VMAIMVAIAVIF 161

[Close]

BLAST of Lag0031227 vs. ExPASy Swiss-Prot
Match: G7L0H3 (Blue copper protein 1a OS=Medicago truncatula OX=3880 GN=MTR_7g086140 PE=2 SV=1)

HSP 1 Score: 82.4 bits (202), Expect = 5.3e-15
Identity = 48/159 (30.19%), Postives = 90/159 (56.60%), Query Frame = 0


Query: 7   LIAVFLLLLCAAVFPVSATDHIVGANRGWNPGINYTLWANNHTFFVGDFISFRYQKSQYN 66
           ++++ ++LL +      ATDHIVG ++GW    +YT WA +  F VGD + F Y  +++N
Sbjct: 9   ILSISMVLLSSVAI---ATDHIVGDDKGWTVDFDYTQWAQDKVFRVGDNLVFNYDPARHN 68

Query: 67  VFEVNQSGYDNCTTEGALGNWSSGKDFILLNNSQRYYFICGNGQCFNGMKVSILVHPL-- 126
           VF+VN + + +CT        S+GKD I L    R +++CG     +  ++ +++  L  
Sbjct: 69  VFKVNGTLFQSCTFPPKNEALSTGKDIIQLKTEGRKWYVCGVADHCSARQMKLVITVLAE 128

Query: 127 -PPAPSSSPNSTSPSSATARAGSRQWRIRALLISLALVW 163
             PAPS  P+S + S  ++  G     + A+++++A+++
Sbjct: 129 GAPAPSPPPSSDAHSVVSSLFGV----VMAIMVAIAVIF 160

[Close]

BLAST of Lag0031227 vs. ExPASy Swiss-Prot
Match: Q41001 (Blue copper protein OS=Pisum sativum OX=3888 PE=2 SV=1)

HSP 1 Score: 81.3 bits (199), Expect = 1.2e-14
Identity = 62/180 (34.44%), Postives = 92/180 (51.11%), Query Frame = 0


Query: 4   SPQLIAVFLLLLCAAVFPVSATDHIVGANRGWNPGINYTLWANNHTFFVGDFISFRYQKS 63
           S  L+  FLL +     P  AT + VG   GW  G +Y+ WA++ TF VGD + F Y   
Sbjct: 4   SNALVLCFLLAIINMALPSLATVYTVGDTSGWVIGGDYSTWASDKTFAVGDSLVFNYGAG 63

Query: 64  QYNVFEVNQSGYDNCTTEGALGNWSSGKDFILLNNSQRYYFICG-NGQCFNGMKVSILVH 123
            + V EV +S Y +CT+  ++   S+G   I L  + ++YFICG  G    GMK+SI V 
Sbjct: 64  AHTVDEVKESDYKSCTSGNSISTDSTGATTIPLKKAGKHYFICGVPGHSTGGMKLSIKVK 123

Query: 124 P---LPPAPSSSPNST---SPSS-------ATARAGSRQWRIRALLIS-LALVWFGLVWI 169
                  APS++P+S+   SPSS        T    ++Q    A  +S +  ++F + WI
Sbjct: 124 ASSGSSAAPSATPSSSGKGSPSSDDTPAATTTTTTPTKQNESSATSLSPIVALFFTVSWI 183

[Close]

BLAST of Lag0031227 vs. ExPASy Swiss-Prot
Match: A0A0M4FTF3 (Blue copper protein OS=Petunia hybrida OX=4102 GN=BCP PE=2 SV=1)

HSP 1 Score: 75.5 bits (184), Expect = 6.5e-13
Identity = 41/124 (33.06%), Postives = 65/124 (52.42%), Query Frame = 0


Query: 17  AAVFPVSATDHIVGANRGWNPGINYTLWANNHTFFVGDFISFRYQKSQYNVFEVNQSGYD 76
           A   P   T++ VG ++GW   ++Y  WA   TF VGD + F+Y K  +NVF+VNQ+G+ 
Sbjct: 108 APASPAPQTEYWVGDDKGWTIDVDYQAWAKGKTFKVGDTLVFKYTKGHHNVFKVNQTGFQ 167

Query: 77  NCTTEGALGNWSSGKDFILLNNSQRYYFICGNGQCFNGMKVSILVHPLPPAPSSSPNSTS 136
           NC         +SG D I L    + ++ICG     +  K  + +  +  AP+ +P   +
Sbjct: 168 NCIAPPPSEGLTSGHDVITLAAPGKKWYICGFPTHCSEHKQKLAI-TVEGAPAQTPPVWA 227

Query: 137 PSSA 141
           P+ A
Sbjct: 228 PAPA 230

The following BLAST results are available for this feature:

BLAST of Lag0031227 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7018121.1	5.7e-75	83.63	Lamin-like protein [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_022934432.1	2.2e-74	83.04	lamin-like protein [Cucurbita moschata]	[more]
XP_023526524.1	2.8e-74	83.04	lamin-like protein [Cucurbita pepo subsp. pepo]	[more]
KAG6581622.1	6.3e-74	83.04	Lamin-like protein, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022983448.1	3.1e-73	82.46	lamin-like protein [Cucurbita maxima]	[more]

BLAST of Lag0031227 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q39131	3.0e-26	39.13	Lamin-like protein OS=Arabidopsis thaliana OX=3702 GN=At5g15350 PE=2 SV=1	[more]
A0A072U307	3.1e-15	30.19	Blue copper protein 1b OS=Medicago truncatula OX=3880 GN=BCP1B PE=2 SV=1	[more]
G7L0H3	5.3e-15	30.19	Blue copper protein 1a OS=Medicago truncatula OX=3880 GN=MTR_7g086140 PE=2 SV=1	[more]
Q41001	1.2e-14	34.44	Blue copper protein OS=Pisum sativum OX=3888 PE=2 SV=1	[more]
A0A0M4FTF3	6.5e-13	33.06	Blue copper protein OS=Petunia hybrida OX=4102 GN=BCP PE=2 SV=1	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01

Position :

Zoom :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003245	Phytocyanin domain	PFAM	PF02298	Cu_bind_like	coord: 35..115 e-value: 1.7E-19 score: 69.6
IPR003245	Phytocyanin domain	PROSITE	PS51485	PHYTOCYANIN	coord: 25..123 score: 36.459953
IPR008972	Cupredoxin	GENE3D	2.60.40.420		coord: 23..124 e-value: 3.1E-30 score: 106.5
IPR008972	Cupredoxin	SUPERFAMILY	49503	Cupredoxins	coord: 24..122
IPR039391	Phytocyanin	PANTHER	PTHR33021	BLUE COPPER PROTEIN	coord: 10..155
None	No IPR available	PANTHER	PTHR33021:SF13	OS09G0557900 PROTEIN	coord: 10..155

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0031227.1	Lag0031227.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0022900	electron transport chain
cellular_component	GO:0046658	anchored component of plasma membrane
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0009055	electron transfer activity