Lag0031212 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0031212
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	RNase H domain-containing protein
Location	chr11: 5833176 .. 5834022 (-)
RNA-Seq Expression	Lag0031212
Synteny	Lag0031212

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTTTTCTGGGAATGTTCTATGGTTAAATCCTTGTGGTGCTGTTCAAAGTTTGCATCCGTTTACAATTCTTTTTCTGGTATGAGTTTTGATTCTCTGTTGTGGGCCTTAAAGGATAAAGTTAGTAGGCTGGATTTTGAGCTATTGGTTGTTTTCTGGTGGGCGATATGGAATTTCCGTAATTCTGTTTTGTGGAATGGGAATTTGATGTAGAAACTATTACAGTGGGCTGAAGACTACTTATCTCCTTTCAGCAGGCGCGGGAAGGCTCTATTGCTTCGGTGGGATTGCAGCAGTCGGCGTGGGTGTTTGAACAGGTCTCGTCGACTGGTGTGCAGCATGTGCCACCGGGTGAGCTGCTGTGGTGTGGGTTCCTCCTCCGATGAATGAACTAAAGTTGAATGTGGATGCTGCAGTGAGGCCGAGACTGGGGCTGCTTGCGGGGGTTTGTATTGCGTAATGAGAGAGGGGAGGTTCTGCTGTCGGGTTGTGAAATTTTGCCCAATTGTTTGAGTGTGGATTTGGCTGAGGGATGGGCCATGCTGAAGGGTATTCTAATTGCTCGACAAATGGGTTTTTTCAGTTTTCATGTTAAAACCGACTCGATGCGATTATCGAAAGTGTTGACAGAAGATGTGGACGATATTTCGGAAGTGGGAGTGATTATGGACAACATTCGAGGTATACTTCATCAAGACTCTGCATGTAAGGTGCTATTCACATATAGGCAAGGCAACATGGTAGCTCATACCATGGCTTCTTTAGCTTTTCGTTATGATAATTTTGTTTGGCTTGAGGATTGGCCTGTTGAGGCCTCTAATGTACTTTTTCGTGATTTATCCTCCTAA

mRNA sequence

ATGTTTTCTGGGAATGTTCTATGGTTAAATCCTTGTGGTGCTGTTCAAAGTTTGCATCCGTTTACAATTCTTTTTCTGGCGCGGGAAGGCTCTATTGCTTCGGTGGGATTGCAGCAGTCGGCGTGGGTGTTTGAACAGGTCTCGTCGACTGGTGTGCAGCATGTGCCACCGGGCCGAGACTGGGGCTGCTTGCGGGGGTTTGTATTGCGTAATGAGAGAGGGGAGGTTCTGCTGTCGGGTTGTGAAATTTTGCCCAATTGTTTGAGTGTGGATTTGGCTGAGGGATGGGCCATGCTGAAGGGTATTCTAATTGCTCGACAAATGGGTTTTTTCAGTTTTCATGTTAAAACCGACTCGATGCGATTATCGAAAGTGTTGACAGAAGATGTGGACGATATTTCGGAAGTGGGAGTGATTATGGACAACATTCGAGGTATACTTCATCAAGACTCTGCATGTAAGGTGCTATTCACATATAGGCAAGGCAACATGGTAGCTCATACCATGGCTTCTTTAGCTTTTCGTTATGATAATTTTGTTTGGCTTGAGGATTGGCCTGTTGAGGCCTCTAATGTACTTTTTCGTGATTTATCCTCCTAA

Coding sequence (CDS)

ATGTTTTCTGGGAATGTTCTATGGTTAAATCCTTGTGGTGCTGTTCAAAGTTTGCATCCGTTTACAATTCTTTTTCTGGCGCGGGAAGGCTCTATTGCTTCGGTGGGATTGCAGCAGTCGGCGTGGGTGTTTGAACAGGTCTCGTCGACTGGTGTGCAGCATGTGCCACCGGGCCGAGACTGGGGCTGCTTGCGGGGGTTTGTATTGCGTAATGAGAGAGGGGAGGTTCTGCTGTCGGGTTGTGAAATTTTGCCCAATTGTTTGAGTGTGGATTTGGCTGAGGGATGGGCCATGCTGAAGGGTATTCTAATTGCTCGACAAATGGGTTTTTTCAGTTTTCATGTTAAAACCGACTCGATGCGATTATCGAAAGTGTTGACAGAAGATGTGGACGATATTTCGGAAGTGGGAGTGATTATGGACAACATTCGAGGTATACTTCATCAAGACTCTGCATGTAAGGTGCTATTCACATATAGGCAAGGCAACATGGTAGCTCATACCATGGCTTCTTTAGCTTTTCGTTATGATAATTTTGTTTGGCTTGAGGATTGGCCTGTTGAGGCCTCTAATGTACTTTTTCGTGATTTATCCTCCTAA

Protein sequence

MFSGNVLWLNPCGAVQSLHPFTILFLAREGSIASVGLQQSAWVFEQVSSTGVQHVPPGRDWGCLRGFVLRNERGEVLLSGCEILPNCLSVDLAEGWAMLKGILIARQMGFFSFHVKTDSMRLSKVLTEDVDDISEVGVIMDNIRGILHQDSACKVLFTYRQGNMVAHTMASLAFRYDNFVWLEDWPVEASNVLFRDLSS

Homology

[Close]

BLAST of Lag0031212 vs. NCBI nr
Match: XP_022150944.1 (uncharacterized protein LOC111018973 [Momordica charantia])

HSP 1 Score: 97.8 bits (242), Expect = 1.1e-16
Identity = 53/136 (38.97%), Postives = 78/136 (57.35%), Query Frame = 0


Query: 66  GFVLRNERGEVLLSGCEILPNCLSVDLAEGWAMLKGILIARQMGFFSFHVKTDSMRLSKV 125
           G ++R+  G V L+   +L     VD  EG+A+ +GIL+A + GF  F ++TDS+R+  +
Sbjct: 130 GVIIRDSTGLVYLTAIRLLARASDVDWVEGFAVYEGILLAVEAGFIRFQIETDSLRIFNL 189

Query: 126 LTEDVDDISEVGVIMDNIRGILHQDS-ACKVLFTYRQGNMVAHTMASLAFRYDNF-VWLE 185
           LT D  D SEVGV+   I+  L   +      FT+R GN  AH +A LA    +  +W+E
Sbjct: 190 LTTDCVDDSEVGVLCSVIKLFLSSHAERVSFSFTHRNGNAXAHLLAQLALTSPHLQIWVE 249

Query: 186 DWPVEASNVLFRDLSS 200
           +WP E S+VL  D  S
Sbjct: 250 EWPDEISSVLAVDCRS 265

[Close]

BLAST of Lag0031212 vs. NCBI nr
Match: XP_022140628.1 (uncharacterized protein LOC111011237 [Momordica charantia])

HSP 1 Score: 87.8 bits (216), Expect = 1.1e-13
Identity = 43/129 (33.33%), Postives = 72/129 (55.81%), Query Frame = 0


Query: 66  GFVLRNERGEVLLSGCEILPNCLSVDLAEGWAMLKGILIARQMGFFSFHVKTDSMRLSKV 125
           G ++RN+RG+V+ S  + L N  SVD+AE    ++G+ +A ++G     ++TDS R+  +
Sbjct: 60  GIIIRNDRGQVMASATKYLENIQSVDMAEAIVAVEGLQLASKIGVNPVILETDSSRIFNL 119

Query: 126 LTEDVDDISEVGVIMDNIRGILHQDSACKVLFTYRQGNMVAHTMASLAFRYDNF-VWLED 185
            ++  +D+SE G I+   +    Q       F  R+GN  AH +A  A     F +W+ED
Sbjct: 120 FSQPSEDLSETGEIVLKAKNFWTQSLHASFNFVKREGNKAAHMLARRALLLREFSIWMED 179

Query: 186 WPVEASNVL 194
           WP+E  + L
Sbjct: 180 WPLELKSCL 188

[Close]

BLAST of Lag0031212 vs. NCBI nr
Match: XP_028115288.1 (uncharacterized protein LOC114313136 isoform X3 [Camellia sinensis])

HSP 1 Score: 86.3 bits (212), Expect = 3.3e-13
Identity = 50/136 (36.76%), Postives = 75/136 (55.15%), Query Frame = 0


Query: 66  GFVLRNERGEVLLSGCEILPNCLSVDLAEGWAMLKGILIARQMGFFSFHVKTDSMRLSKV 125
           G V+RN  GEV+ +  E LP  +  D AE +A  K + +AR +G    H++ DS+R+ KV
Sbjct: 685 GVVIRNHLGEVMAALSERLPFWVDSDCAEAYAATKAVELARDLGLSDIHLEGDSLRIVKV 744

Query: 126 LTEDVDDISEVGVIMDNIRGILHQDSACKVLFTYRQGNMVAHTMASLAFRYDNF--VWLE 185
           L E+ + +SE G I+    G+    S   V    RQGN +AH +A +A R+ N+  VW+E
Sbjct: 745 LREEAEFMSEYGHILLQTVGVCKSFSRFHVSHVGRQGNGLAHGLARMA-RHQNYHQVWME 804

Query: 186 DWPVEASNVLFRDLSS 200
             P     +   D+SS
Sbjct: 805 GLPASLIAIATSDVSS 819

[Close]

BLAST of Lag0031212 vs. NCBI nr
Match: XP_028115297.1 (uncharacterized protein LOC114313136 isoform X6 [Camellia sinensis] >XP_028115298.1 uncharacterized protein LOC114313136 isoform X6 [Camellia sinensis] >XP_028115299.1 uncharacterized protein LOC114313136 isoform X6 [Camellia sinensis] >XP_028115300.1 uncharacterized protein LOC114313136 isoform X6 [Camellia sinensis] >XP_028115301.1 uncharacterized protein LOC114313136 isoform X6 [Camellia sinensis])

HSP 1 Score: 86.3 bits (212), Expect = 3.3e-13
Identity = 50/136 (36.76%), Postives = 75/136 (55.15%), Query Frame = 0


Query: 66  GFVLRNERGEVLLSGCEILPNCLSVDLAEGWAMLKGILIARQMGFFSFHVKTDSMRLSKV 125
           G V+RN  GEV+ +  E LP  +  D AE +A  K + +AR +G    H++ DS+R+ KV
Sbjct: 695 GVVIRNHLGEVMAALSERLPFWVDSDCAEAYAATKAVELARDLGLSDIHLEGDSLRIVKV 754

Query: 126 LTEDVDDISEVGVIMDNIRGILHQDSACKVLFTYRQGNMVAHTMASLAFRYDNF--VWLE 185
           L E+ + +SE G I+    G+    S   V    RQGN +AH +A +A R+ N+  VW+E
Sbjct: 755 LREEAEFMSEYGHILLQTVGVCKSFSRFHVSHVGRQGNGLAHGLARMA-RHQNYHQVWME 814

Query: 186 DWPVEASNVLFRDLSS 200
             P     +   D+SS
Sbjct: 815 GLPASLIAIATSDVSS 829

[Close]

BLAST of Lag0031212 vs. NCBI nr
Match: XP_028115286.1 (uncharacterized protein LOC114313136 isoform X1 [Camellia sinensis])

HSP 1 Score: 86.3 bits (212), Expect = 3.3e-13
Identity = 50/136 (36.76%), Postives = 75/136 (55.15%), Query Frame = 0


Query: 66  GFVLRNERGEVLLSGCEILPNCLSVDLAEGWAMLKGILIARQMGFFSFHVKTDSMRLSKV 125
           G V+RN  GEV+ +  E LP  +  D AE +A  K + +AR +G    H++ DS+R+ KV
Sbjct: 728 GVVIRNHLGEVMAALSERLPFWVDSDCAEAYAATKAVELARDLGLSDIHLEGDSLRIVKV 787

Query: 126 LTEDVDDISEVGVIMDNIRGILHQDSACKVLFTYRQGNMVAHTMASLAFRYDNF--VWLE 185
           L E+ + +SE G I+    G+    S   V    RQGN +AH +A +A R+ N+  VW+E
Sbjct: 788 LREEAEFMSEYGHILLQTVGVCKSFSRFHVSHVGRQGNGLAHGLARMA-RHQNYHQVWME 847

Query: 186 DWPVEASNVLFRDLSS 200
             P     +   D+SS
Sbjct: 848 GLPASLIAIATSDVSS 862

[Close]

BLAST of Lag0031212 vs. ExPASy TrEMBL
Match: A0A6J1DBJ7 (uncharacterized protein LOC111018973 OS=Momordica charantia OX=3673 GN=LOC111018973 PE=4 SV=1)

HSP 1 Score: 97.8 bits (242), Expect = 5.3e-17
Identity = 53/136 (38.97%), Postives = 78/136 (57.35%), Query Frame = 0


Query: 66  GFVLRNERGEVLLSGCEILPNCLSVDLAEGWAMLKGILIARQMGFFSFHVKTDSMRLSKV 125
           G ++R+  G V L+   +L     VD  EG+A+ +GIL+A + GF  F ++TDS+R+  +
Sbjct: 130 GVIIRDSTGLVYLTAIRLLARASDVDWVEGFAVYEGILLAVEAGFIRFQIETDSLRIFNL 189

Query: 126 LTEDVDDISEVGVIMDNIRGILHQDS-ACKVLFTYRQGNMVAHTMASLAFRYDNF-VWLE 185
           LT D  D SEVGV+   I+  L   +      FT+R GN  AH +A LA    +  +W+E
Sbjct: 190 LTTDCVDDSEVGVLCSVIKLFLSSHAERVSFSFTHRNGNAXAHLLAQLALTSPHLQIWVE 249

Query: 186 DWPVEASNVLFRDLSS 200
           +WP E S+VL  D  S
Sbjct: 250 EWPDEISSVLAVDCRS 265

[Close]

BLAST of Lag0031212 vs. ExPASy TrEMBL
Match: A0A6J1CIF1 (uncharacterized protein LOC111011237 OS=Momordica charantia OX=3673 GN=LOC111011237 PE=4 SV=1)

HSP 1 Score: 87.8 bits (216), Expect = 5.5e-14
Identity = 43/129 (33.33%), Postives = 72/129 (55.81%), Query Frame = 0


Query: 66  GFVLRNERGEVLLSGCEILPNCLSVDLAEGWAMLKGILIARQMGFFSFHVKTDSMRLSKV 125
           G ++RN+RG+V+ S  + L N  SVD+AE    ++G+ +A ++G     ++TDS R+  +
Sbjct: 60  GIIIRNDRGQVMASATKYLENIQSVDMAEAIVAVEGLQLASKIGVNPVILETDSSRIFNL 119

Query: 126 LTEDVDDISEVGVIMDNIRGILHQDSACKVLFTYRQGNMVAHTMASLAFRYDNF-VWLED 185
            ++  +D+SE G I+   +    Q       F  R+GN  AH +A  A     F +W+ED
Sbjct: 120 FSQPSEDLSETGEIVLKAKNFWTQSLHASFNFVKREGNKAAHMLARRALLLREFSIWMED 179

Query: 186 WPVEASNVL 194
           WP+E  + L
Sbjct: 180 WPLELKSCL 188

[Close]

BLAST of Lag0031212 vs. ExPASy TrEMBL
Match: A0A7J7GYW5 (Uncharacterized protein OS=Camellia sinensis OX=4442 GN=HYC85_016191 PE=3 SV=1)

HSP 1 Score: 84.0 bits (206), Expect = 8.0e-13
Identity = 49/136 (36.03%), Postives = 74/136 (54.41%), Query Frame = 0


Query: 66  GFVLRNERGEVLLSGCEILPNCLSVDLAEGWAMLKGILIARQMGFFSFHVKTDSMRLSKV 125
           G V+RN  GEV+ +  E LP  +  D AE +A  K + +AR +GF   H++ DS+R+ K 
Sbjct: 694 GVVIRNHLGEVMAALSERLPFWVDSDCAEAYAATKAVELARDLGFSDIHLEGDSLRIVKA 753

Query: 126 LTEDVDDISEVGVIMDNIRGILHQDSACKVLFTYRQGNMVAHTMASLAFRYDNF--VWLE 185
           L E+ + +SE G I+    G     S   V    RQGN +AH +A +A R+ N+  VW+ 
Sbjct: 754 LREEAEFMSEYGHILLQTVGACKSFSRFHVSHVGRQGNGLAHGLARMA-RHQNYQQVWMG 813

Query: 186 DWPVEASNVLFRDLSS 200
           D P     +   ++SS
Sbjct: 814 DLPASLIAIATSEVSS 828

[Close]

BLAST of Lag0031212 vs. ExPASy TrEMBL
Match: A0A7J7G0B6 (RNase H domain-containing protein OS=Camellia sinensis OX=4442 GN=HYC85_030294 PE=4 SV=1)

HSP 1 Score: 77.0 bits (188), Expect = 9.7e-11
Identity = 45/132 (34.09%), Postives = 71/132 (53.79%), Query Frame = 0


Query: 66  GFVLRNERGEVLLSGCEILPNCLSVDLAEGWAMLKGILIARQMGFFSFHVKTDSMRLSKV 125
           G V+RN  GEV+ +  E LP  +  D AE +A  K I +A+ +GF    ++ DS+ + K 
Sbjct: 170 GVVIRNSMGEVMGAMSEQLPFWVDADCAEAFAAAKAIELAKYLGFSDIILEGDSLNIVKA 229

Query: 126 LTEDVDDISEVGVIMDNIRGILHQDSACKVLFTYRQGNMVAHTMASLAFRYDN-FVWLED 185
           L EDV+ + E G ++  + G      + +V    R GNM+AH +A +A   D+  VW+E+
Sbjct: 230 LWEDVELLPEYGHLVSQVVGASRLFRSFQVCHVGRMGNMLAHGLAWMARDLDHQLVWMEE 289

Query: 186 WPVEASNVLFRD 197
            P     V+  D
Sbjct: 290 VPQALIEVVAAD 301

[Close]

BLAST of Lag0031212 vs. ExPASy TrEMBL
Match: A0A803LHG6 (Uncharacterized protein OS=Chenopodium quinoa OX=63459 PE=4 SV=1)

HSP 1 Score: 76.6 bits (187), Expect = 1.3e-10
Identity = 41/135 (30.37%), Postives = 72/135 (53.33%), Query Frame = 0


Query: 66  GFVLRNERGEVLLSGCEILPNCLSVDLAEGWAMLKGILIARQMGFFSFHVKTDSMRLSKV 125
           G V+R+  G+V+ + C  +     VD+ E  AM   + IA + GF    ++TD+++L   
Sbjct: 229 GGVMRDNVGDVVAATCLQIVGRFDVDVVEAMAMRHALKIAMESGFVRLCLETDNLKLHSH 288

Query: 126 LTEDVDDISEVGVIMDNIRGILHQDSACKVLFTYRQGNMVAHTMASLAFRYDNF-VWLED 185
           L +   D +  G+++ +I  +     +C   F  R+GN VAH++A L+  +D   VWLE+
Sbjct: 289 LKKSKYDQTTFGIVVKDILTLASSCHSCVFEFVKREGNKVAHSLAKLSSTFDTLRVWLEE 348

Query: 186 WPVEASNVLFRDLSS 200
           +P     ++  D SS
Sbjct: 349 YPSGIHELVMADSSS 363

[Close]

BLAST of Lag0031212 vs. TAIR 10
Match: AT4G29090.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 50.8 bits (120), Expect = 1.4e-06
Identity = 38/117 (32.48%), Postives = 58/117 (49.57%), Query Frame = 0


Query: 63  CLRGFVLRNERGEVLLSGCEILPNCLSVDLAEGWAMLKGILIARQMGFFSFHVKTDSMRL 122
           C  G+VLRNE+GEV   G   LP   SV  AE  AM   +L   +  +     ++DS  L
Sbjct: 443 CGIGWVLRNEKGEVKWMGARALPKLKSVLEAELEAMRWAVLSLSRFQYNYVIFESDSQVL 502

Query: 123 SKVLTEDVDDISEVGVIMDNIRGILHQDSACKVLFTYRQGNMVAHTMASLAFRYDNF 180
            ++L  D    S    I D ++ +L Q +  K +F  R+GN +A  +A  +  + N+
Sbjct: 503 IEILNNDEIWPSLKPTIQD-LQRLLSQFTEVKFVFIPREGNTLAERVARESLSFLNY 558

The following BLAST results are available for this feature:

BLAST of Lag0031212 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022150944.1	1.1e-16	38.97	uncharacterized protein LOC111018973 [Momordica charantia]	[more]
XP_022140628.1	1.1e-13	33.33	uncharacterized protein LOC111011237 [Momordica charantia]	[more]
XP_028115288.1	3.3e-13	36.76	uncharacterized protein LOC114313136 isoform X3 [Camellia sinensis]	[more]
XP_028115297.1	3.3e-13	36.76	uncharacterized protein LOC114313136 isoform X6 [Camellia sinensis] >XP_02811529...	[more]
XP_028115286.1	3.3e-13	36.76	uncharacterized protein LOC114313136 isoform X1 [Camellia sinensis]	[more]

BLAST of Lag0031212 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of Lag0031212 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DBJ7	5.3e-17	38.97	uncharacterized protein LOC111018973 OS=Momordica charantia OX=3673 GN=LOC111018...	[more]
A0A6J1CIF1	5.5e-14	33.33	uncharacterized protein LOC111011237 OS=Momordica charantia OX=3673 GN=LOC111011...	[more]
A0A7J7GYW5	8.0e-13	36.03	Uncharacterized protein OS=Camellia sinensis OX=4442 GN=HYC85_016191 PE=3 SV=1	[more]
A0A7J7G0B6	9.7e-11	34.09	RNase H domain-containing protein OS=Camellia sinensis OX=4442 GN=HYC85_030294 P...	[more]
A0A803LHG6	1.3e-10	30.37	Uncharacterized protein OS=Chenopodium quinoa OX=63459 PE=4 SV=1	[more]

BLAST of Lag0031212 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G29090.1	1.4e-06	32.48	Ribonuclease H-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036397	Ribonuclease H superfamily	GENE3D	3.30.420.10		coord: 57..175 e-value: 1.4E-6 score: 30.4
IPR002156	Ribonuclease H domain	PFAM	PF13456	RVT_3	coord: 66..173 e-value: 6.5E-19 score: 68.0
None	No IPR available	PANTHER	PTHR47723	OS05G0353850 PROTEIN	coord: 66..188
IPR044730	Ribonuclease H-like domain, plant type	CDD	cd06222	RNase_H_like	coord: 66..170 e-value: 4.08308E-10 score: 53.472

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0031212.1	Lag0031212.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0004523	RNA-DNA hybrid ribonuclease activity