Homology
BLAST of Lag0031211 vs. NCBI nr
Match:
KAG6581611.1 (4-coumarate--CoA ligase 1, partial [Cucurbita argyrosperma subsp. sororia])
HSP 1 Score: 1002.7 bits (2591), Expect = 1.3e-288
Identity = 503/546 (92.12%), Postives = 525/546 (96.15%), Query Frame = 0
Query: 1 MAIETLQKEFIFRSKLPDIYIPNHLPLHSYCLQDNKAKIGHRTCLVNGVTGESFTYDDVD 60
MA+E LQ +FIFRSKLPDIYIPNHLPLHSYCL +N AKIGHRTCL+N VTGESFTY DVD
Sbjct: 1 MAVEALQNDFIFRSKLPDIYIPNHLPLHSYCLHENLAKIGHRTCLINSVTGESFTYHDVD 60
Query: 61 LAARKVASGLNKLGIAKCDVIMLLLPNSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQ 120
LAARKVASGLNKLGIA+ DVIMLLL NSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQ
Sbjct: 61 LAARKVASGLNKLGIAQGDVIMLLLQNSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQ 120
Query: 121 AKGSKAKLIVTQASYYEKVKDITEELPDVKIMTVDSSPDGCLSFADLIQADEGEVAAVEI 180
AKGS AKLIVTQ+SYYEKVK+ITEELPDVKIMTVDS PDGCLSFADLIQADE E+ VEI
Sbjct: 121 AKGSNAKLIVTQSSYYEKVKEITEELPDVKIMTVDSPPDGCLSFADLIQADEREMPTVEI 180
Query: 181 HPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFRNDDVILCVLPLFHI 240
+PDDVVALPYSSGTTGLPKGVMLTH+GLVTSVAQQVDGENPNLY+RN+DVILCVLPLFHI
Sbjct: 181 NPDDVVALPYSSGTTGLPKGVMLTHRGLVTSVAQQVDGENPNLYYRNEDVILCVLPLFHI 240
Query: 241 YSLNSVLLCGLRAGATILIMPKFEIGSLLKLVEKYRVSIAPIVPPIVLAIAKSPDLDKYD 300
YSLNSVLLCGLRAG TILIMPKFEIGSLL+LV+K++VSIAPIVPPIVLAIAKSPDLDKYD
Sbjct: 241 YSLNSVLLCGLRAGTTILIMPKFEIGSLLQLVQKFKVSIAPIVPPIVLAIAKSPDLDKYD 300
Query: 301 LSSIRMIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360
LSSIR+IK GGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG
Sbjct: 301 LSSIRVIKCGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360
Query: 361 ACGTVVRNAEMKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDEDGWLHT 420
ACGTVVRNAEMKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATID+DGWLHT
Sbjct: 361 ACGTVVRNAEMKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT 420
Query: 421 GDIGLIDEDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEV 480
GDIG ID+DDELFIVDRLKELIKYKGFQVAPAE+EALLLTHP ISDAAVVPMKDE+AGEV
Sbjct: 421 GDIGFIDDDDELFIVDRLKELIKYKGFQVAPAEVEALLLTHPAISDAAVVPMKDEEAGEV 480
Query: 481 PVAFVVKLKNSEVTEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKLAT 540
PVAFVV+LKNSEVTEDEIKQFISKQVVFYKRI R F IDAIPKSPSGKILRKELRAKLAT
Sbjct: 481 PVAFVVRLKNSEVTEDEIKQFISKQVVFYKRIKRAFMIDAIPKSPSGKILRKELRAKLAT 540
Query: 541 DFPNSN 547
FPNSN
Sbjct: 541 GFPNSN 546
BLAST of Lag0031211 vs. NCBI nr
Match:
XP_022935389.1 (4-coumarate--CoA ligase 1-like [Cucurbita moschata])
HSP 1 Score: 1000.0 bits (2584), Expect = 8.1e-288
Identity = 501/546 (91.76%), Postives = 524/546 (95.97%), Query Frame = 0
Query: 1 MAIETLQKEFIFRSKLPDIYIPNHLPLHSYCLQDNKAKIGHRTCLVNGVTGESFTYDDVD 60
MA+E LQ +FIFRSKLPDIYIPNHLPLHSYCL +N AKIGHRTCL+N VTGESFTY DVD
Sbjct: 1 MAVEALQNDFIFRSKLPDIYIPNHLPLHSYCLHENLAKIGHRTCLINSVTGESFTYHDVD 60
Query: 61 LAARKVASGLNKLGIAKCDVIMLLLPNSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQ 120
LAARKVASGLNKLGIA+ DVIML+L NSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQ
Sbjct: 61 LAARKVASGLNKLGIAQGDVIMLILQNSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQ 120
Query: 121 AKGSKAKLIVTQASYYEKVKDITEELPDVKIMTVDSSPDGCLSFADLIQADEGEVAAVEI 180
AKGS AKLIVTQ+SYYEKVK+ITEELPDVKIMTVDS PDGCLSFADLIQADE E+ VEI
Sbjct: 121 AKGSNAKLIVTQSSYYEKVKEITEELPDVKIMTVDSPPDGCLSFADLIQADEREMPTVEI 180
Query: 181 HPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFRNDDVILCVLPLFHI 240
+PDDVVALPYSSGTTGLPKGVMLTH+GLVTSVAQQVDGENPNLY+RN+DVILCVLPLFHI
Sbjct: 181 NPDDVVALPYSSGTTGLPKGVMLTHRGLVTSVAQQVDGENPNLYYRNEDVILCVLPLFHI 240
Query: 241 YSLNSVLLCGLRAGATILIMPKFEIGSLLKLVEKYRVSIAPIVPPIVLAIAKSPDLDKYD 300
YSLNSVLLCGLRAG TILIMPKFEIGSLL+LV+K++VSIAPIVPPIVLAIAKSPDLDKYD
Sbjct: 241 YSLNSVLLCGLRAGTTILIMPKFEIGSLLQLVQKFKVSIAPIVPPIVLAIAKSPDLDKYD 300
Query: 301 LSSIRMIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360
LSSIR+IK GGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG
Sbjct: 301 LSSIRVIKCGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360
Query: 361 ACGTVVRNAEMKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDEDGWLHT 420
ACGTVVRNAEMKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATID+DGWLHT
Sbjct: 361 ACGTVVRNAEMKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT 420
Query: 421 GDIGLIDEDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEV 480
GDIG ID+DDELFIVDRLKELIKYKGFQVAPAE+EALLLTHP ISDAAVVPMKDE+AGEV
Sbjct: 421 GDIGFIDDDDELFIVDRLKELIKYKGFQVAPAEVEALLLTHPAISDAAVVPMKDEEAGEV 480
Query: 481 PVAFVVKLKNSEVTEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKLAT 540
PVAFVV+LKNSEVTEDEIKQFISKQVVFYKRI R F IDAIPKSPSGKILRKELRAKLA
Sbjct: 481 PVAFVVRLKNSEVTEDEIKQFISKQVVFYKRIKRAFMIDAIPKSPSGKILRKELRAKLAA 540
Query: 541 DFPNSN 547
FPNSN
Sbjct: 541 GFPNSN 546
BLAST of Lag0031211 vs. NCBI nr
Match:
XP_023526871.1 (4-coumarate--CoA ligase 1-like isoform X1 [Cucurbita pepo subsp. pepo])
HSP 1 Score: 997.3 bits (2577), Expect = 5.3e-287
Identity = 500/546 (91.58%), Postives = 523/546 (95.79%), Query Frame = 0
Query: 1 MAIETLQKEFIFRSKLPDIYIPNHLPLHSYCLQDNKAKIGHRTCLVNGVTGESFTYDDVD 60
MA+E LQ +FIFRSKLP IYIPNHLPLHSYCL +N AKIGHRTCL+N VTGESFTY DVD
Sbjct: 1 MAVEALQNDFIFRSKLPHIYIPNHLPLHSYCLHENLAKIGHRTCLINSVTGESFTYHDVD 60
Query: 61 LAARKVASGLNKLGIAKCDVIMLLLPNSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQ 120
LAARKVASGLNKLGIA+ DVIMLLL NSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQ
Sbjct: 61 LAARKVASGLNKLGIAQGDVIMLLLQNSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQ 120
Query: 121 AKGSKAKLIVTQASYYEKVKDITEELPDVKIMTVDSSPDGCLSFADLIQADEGEVAAVEI 180
AKGS AKLIVTQ+SYYEKVK+ITEE+PDVKIMTVDS PDGCLSFADLIQADE E+ VEI
Sbjct: 121 AKGSNAKLIVTQSSYYEKVKEITEEVPDVKIMTVDSPPDGCLSFADLIQADEREMPTVEI 180
Query: 181 HPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFRNDDVILCVLPLFHI 240
+PDDVVALPYSSGTTGLPKGVMLTH+GLVTSVAQQVDGENPNLY+RN+DVILCVLPLFHI
Sbjct: 181 NPDDVVALPYSSGTTGLPKGVMLTHRGLVTSVAQQVDGENPNLYYRNEDVILCVLPLFHI 240
Query: 241 YSLNSVLLCGLRAGATILIMPKFEIGSLLKLVEKYRVSIAPIVPPIVLAIAKSPDLDKYD 300
YSLNSVLLCGLRAG TILIMPKFEIGSLL+LV+K++VSIAPIVPPIVLAIAKSPDLDKYD
Sbjct: 241 YSLNSVLLCGLRAGTTILIMPKFEIGSLLQLVQKFKVSIAPIVPPIVLAIAKSPDLDKYD 300
Query: 301 LSSIRMIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360
LSSIR+IK GGAPLGKELEDTVR KFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG
Sbjct: 301 LSSIRVIKCGGAPLGKELEDTVRTKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360
Query: 361 ACGTVVRNAEMKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDEDGWLHT 420
ACGTVVRNAEMKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATID+DGWLHT
Sbjct: 361 ACGTVVRNAEMKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT 420
Query: 421 GDIGLIDEDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEV 480
GDIG ID+DDELFIVDRLKELIKYKGFQVAPAE+EALLLTHP ISDAAVVPMKDE+AGEV
Sbjct: 421 GDIGFIDDDDELFIVDRLKELIKYKGFQVAPAEVEALLLTHPAISDAAVVPMKDEEAGEV 480
Query: 481 PVAFVVKLKNSEVTEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKLAT 540
PVAFVV+LKNSEVTEDEIKQFISKQVVFYKRI R F IDAIPKSPSGKILRKELRAKLAT
Sbjct: 481 PVAFVVRLKNSEVTEDEIKQFISKQVVFYKRIKRAFMIDAIPKSPSGKILRKELRAKLAT 540
Query: 541 DFPNSN 547
FPNSN
Sbjct: 541 GFPNSN 546
BLAST of Lag0031211 vs. NCBI nr
Match:
XP_023524772.1 (4-coumarate--CoA ligase 1-like [Cucurbita pepo subsp. pepo])
HSP 1 Score: 993.8 bits (2568), Expect = 5.8e-286
Identity = 504/546 (92.31%), Postives = 522/546 (95.60%), Query Frame = 0
Query: 1 MAIETLQKEFIFRSKLPDIYIPNHLPLHSYCLQDNKAKIGHRTCLVNGVTGESFTYDDVD 60
MA ET+ +FIFRSKLPDIYIP HLPLHSYCLQ+ A+IGHRTCL+N VTGESFTY DVD
Sbjct: 1 MATETVH-DFIFRSKLPDIYIPKHLPLHSYCLQEKMAEIGHRTCLINAVTGESFTYSDVD 60
Query: 61 LAARKVASGLNKLGIAKCDVIMLLLPNSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQ 120
LAARK ASGL KLGIAK DVIMLLLPNSPEFVFAFLGASY GAIMTAANPFFTAAEIAKQ
Sbjct: 61 LAARKAASGLYKLGIAKGDVIMLLLPNSPEFVFAFLGASYLGAIMTAANPFFTAAEIAKQ 120
Query: 121 AKGSKAKLIVTQASYYEKVKDITEELPDVKIMTVDSSPDGCLSFADLIQADEGEVAAVEI 180
AKGSKAKLI+TQ+SYYEKVK+ITE+LPD KIMTVDS P GCLSFADLIQ + E+ AVEI
Sbjct: 121 AKGSKAKLIITQSSYYEKVKEITEQLPDAKIMTVDSPPLGCLSFADLIQGGDHEIPAVEI 180
Query: 181 HPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFRNDDVILCVLPLFHI 240
PDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLY+RNDDVILCVLPLFHI
Sbjct: 181 SPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYYRNDDVILCVLPLFHI 240
Query: 241 YSLNSVLLCGLRAGATILIMPKFEIGSLLKLVEKYRVSIAPIVPPIVLAIAKSPDLDKYD 300
YSLNSVLLCGLRAGATILIMPKFEIGSLL+LVEKYRVSIAPIVPPIVLAIAKSPDL+KYD
Sbjct: 241 YSLNSVLLCGLRAGATILIMPKFEIGSLLQLVEKYRVSIAPIVPPIVLAIAKSPDLEKYD 300
Query: 301 LSSIRMIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360
LSSIRMIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG
Sbjct: 301 LSSIRMIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360
Query: 361 ACGTVVRNAEMKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDEDGWLHT 420
ACGTVVRNAE+KIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATID +GWLHT
Sbjct: 361 ACGTVVRNAEIKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDSEGWLHT 420
Query: 421 GDIGLIDEDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEV 480
GDIGLID+DDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDE+AGEV
Sbjct: 421 GDIGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEEAGEV 480
Query: 481 PVAFVVKLKNSEVTEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKLAT 540
PVAFVVKLKNSEVTEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKLA
Sbjct: 481 PVAFVVKLKNSEVTEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKLAA 540
Query: 541 DFPNSN 547
DFP N
Sbjct: 541 DFPIPN 545
BLAST of Lag0031211 vs. NCBI nr
Match:
XP_022983944.1 (4-coumarate--CoA ligase 1-like [Cucurbita maxima])
HSP 1 Score: 991.9 bits (2563), Expect = 2.2e-285
Identity = 499/546 (91.39%), Postives = 522/546 (95.60%), Query Frame = 0
Query: 1 MAIETLQKEFIFRSKLPDIYIPNHLPLHSYCLQDNKAKIGHRTCLVNGVTGESFTYDDVD 60
MA+E LQ +FIFRSKLPDIYIPNHLPLHSYCL +N AKIGHRTCL+N VT ESFTY DVD
Sbjct: 1 MAVEALQNDFIFRSKLPDIYIPNHLPLHSYCLHENLAKIGHRTCLINAVTAESFTYHDVD 60
Query: 61 LAARKVASGLNKLGIAKCDVIMLLLPNSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQ 120
LAARKVASGLNKLGIA+ DVI+LLL NSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQ
Sbjct: 61 LAARKVASGLNKLGIAQGDVILLLLQNSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQ 120
Query: 121 AKGSKAKLIVTQASYYEKVKDITEELPDVKIMTVDSSPDGCLSFADLIQADEGEVAAVEI 180
AKGS AKLIVTQ+SYYEKVK+ITEELPDVKIMTVDS PDGCLSFADLIQADE E+ VEI
Sbjct: 121 AKGSNAKLIVTQSSYYEKVKEITEELPDVKIMTVDSPPDGCLSFADLIQADEREMPTVEI 180
Query: 181 HPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFRNDDVILCVLPLFHI 240
+PDDVVALPYSSGTTGLPKGVMLTH+GLVTSVAQQVDGENPNLY+RN+DVILCVLPLFHI
Sbjct: 181 NPDDVVALPYSSGTTGLPKGVMLTHRGLVTSVAQQVDGENPNLYYRNEDVILCVLPLFHI 240
Query: 241 YSLNSVLLCGLRAGATILIMPKFEIGSLLKLVEKYRVSIAPIVPPIVLAIAKSPDLDKYD 300
YSLNSVLLCGLRAG TILIMPKFEIGSLL+LV+K++VSIAPIVPPIVLAIAKSPDLDKYD
Sbjct: 241 YSLNSVLLCGLRAGTTILIMPKFEIGSLLQLVQKFKVSIAPIVPPIVLAIAKSPDLDKYD 300
Query: 301 LSSIRMIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360
LSSIR+IK GGAPLGKELED+VRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG
Sbjct: 301 LSSIRVIKCGGAPLGKELEDSVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360
Query: 361 ACGTVVRNAEMKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDEDGWLHT 420
ACGTVVRNAEMKIVDTENG SLPRNTPGEICIRGDQIMKGYLNNPEATAATID+DGWLHT
Sbjct: 361 ACGTVVRNAEMKIVDTENGCSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT 420
Query: 421 GDIGLIDEDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEV 480
GDIG ID+DDELFIVDRLKELIKYKGFQVAPAELEALLLTHP ISDAAVVPMKDE+AGEV
Sbjct: 421 GDIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPAISDAAVVPMKDEEAGEV 480
Query: 481 PVAFVVKLKNSEVTEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKLAT 540
PVAFVV+LKNSEVTEDEIKQFISKQVVFYKRI R F IDAIPKSPSGKILRKELRAKLAT
Sbjct: 481 PVAFVVRLKNSEVTEDEIKQFISKQVVFYKRIKRAFMIDAIPKSPSGKILRKELRAKLAT 540
Query: 541 DFPNSN 547
FP SN
Sbjct: 541 GFPISN 546
BLAST of Lag0031211 vs. ExPASy Swiss-Prot
Match:
O24146 (4-coumarate--CoA ligase 2 OS=Nicotiana tabacum OX=4097 GN=4CL2 PE=1 SV=1)
HSP 1 Score: 807.7 bits (2085), Expect = 7.8e-233
Identity = 408/544 (75.00%), Postives = 459/544 (84.38%), Query Frame = 0
Query: 1 MAIETLQKEFIFRSKLPDIYIPNHLPLHSYCLQDNKAKIGHRTCLVNGVTGESFTYDDVD 60
M +T Q + IFRSKLPDIYIPNHLPLHSYC +N ++ R CL+NG + +TY DV+
Sbjct: 1 MEKDTKQVDIIFRSKLPDIYIPNHLPLHSYCF-ENISEFSSRPCLINGANKQIYTYADVE 60
Query: 61 LAARKVASGLNKLGIAKCDVIMLLLPNSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQ 120
L +RKVA+GL+K GI D IM+LLPNSPEFVFAF+GASY GAI T ANP FT AE+ KQ
Sbjct: 61 LNSRKVAAGLHKQGIQPKDTIMILLPNSPEFVFAFIGASYLGAISTMANPLFTPAEVVKQ 120
Query: 121 AKGSKAKLIVTQASYYEKVKDITEELPDVKIMTVDSSPDGCLSFADLIQADEGEVAAVEI 180
AK S AK+IVTQA + KVKD E DVKI+ +DS+P+GCL F+ L QA+E ++ VEI
Sbjct: 121 AKASSAKIIVTQACHVNKVKDYAFE-NDVKIICIDSAPEGCLHFSVLTQANEHDIPEVEI 180
Query: 181 HPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFRNDDVILCVLPLFHI 240
PDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLY ++DV+LCVLPLFHI
Sbjct: 181 QPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYIHSEDVMLCVLPLFHI 240
Query: 241 YSLNSVLLCGLRAGATILIMPKFEIGSLLKLVEKYRVSIAPIVPPIVLAIAKSPDLDKYD 300
YSLNSVLLCGLR GA ILIM KF+I S L+L+++Y+V+I P VPPIVLAIAKSP +D YD
Sbjct: 241 YSLNSVLLCGLRVGAAILIMQKFDIVSFLELIQRYKVTIGPFVPPIVLAIAKSPMVDDYD 300
Query: 301 LSSIRMIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360
LSS+R + SG APLGKELEDTVRAKFP A LGQGYGMTEAGPVL M LAFAKEPF +K G
Sbjct: 301 LSSVRTVMSGAAPLGKELEDTVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFEIKSG 360
Query: 361 ACGTVVRNAEMKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDEDGWLHT 420
ACGTVVRNAEMKIVD + G SLPRN GEICIRGDQIMKGYLN+PEATA TID++GWL+T
Sbjct: 361 ACGTVVRNAEMKIVDPKTGNSLPRNQSGEICIRGDQIMKGYLNDPEATARTIDKEGWLYT 420
Query: 421 GDIGLIDEDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEV 480
GDIG ID+DDELFIVDRLKELIKYKGFQVAPAELEALLL HP ISDAAVVPMKDEQAGEV
Sbjct: 421 GDIGYIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLNHPNISDAAVVPMKDEQAGEV 480
Query: 481 PVAFVVKLKNSEVTEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKLAT 540
PVAFVV+ S +TEDE+K FISKQV+FYKRI RVFF+DAIPKSPSGKILRK+LRAKLA
Sbjct: 481 PVAFVVRSNGSTITEDEVKDFISKQVIFYKRIKRVFFVDAIPKSPSGKILRKDLRAKLAA 540
Query: 541 DFPN 545
PN
Sbjct: 541 GLPN 542
BLAST of Lag0031211 vs. ExPASy Swiss-Prot
Match:
O24145 (4-coumarate--CoA ligase 1 OS=Nicotiana tabacum OX=4097 GN=4CL1 PE=2 SV=1)
HSP 1 Score: 799.7 bits (2064), Expect = 2.1e-230
Identity = 397/542 (73.25%), Postives = 453/542 (83.58%), Query Frame = 0
Query: 4 ETLQK-EFIFRSKLPDIYIPNHLPLHSYCLQDNKAKIGHRTCLVNGVTGESFTYDDVDLA 63
ET Q + IFRSKLPDIYIP HLPLHSYC +N ++ R CL+NG + +TY +V+L
Sbjct: 8 ETKQSGDLIFRSKLPDIYIPKHLPLHSYCF-ENISEFSSRPCLINGANDQIYTYAEVELT 67
Query: 64 ARKVASGLNKLGIAKCDVIMLLLPNSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQAK 123
RKVA GLNKLGI + D IM+LLPNSPEFVFAF+GASY GAI T ANP FT AE+ KQAK
Sbjct: 68 CRKVAVGLNKLGIQQKDTIMILLPNSPEFVFAFMGASYLGAISTMANPLFTPAEVVKQAK 127
Query: 124 GSKAKLIVTQASYYEKVKDITEELPDVKIMTVDSSPDGCLSFADLIQADEGEVAAVEIHP 183
S AK+I+TQ+ + KVKD E DVK++ +DS+P+GCL F++L Q+DE E+ V+I P
Sbjct: 128 ASSAKIIITQSCFVGKVKDYASE-NDVKVICIDSAPEGCLHFSELTQSDEHEIPEVKIQP 187
Query: 184 DDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFRNDDVILCVLPLFHIYS 243
DDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGEN NLY ++DV++CVLPLFHIYS
Sbjct: 188 DDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSEDVLMCVLPLFHIYS 247
Query: 244 LNSVLLCGLRAGATILIMPKFEIGSLLKLVEKYRVSIAPIVPPIVLAIAKSPDLDKYDLS 303
LNS+LLCGLR GA ILIM KF+I L+L++KY+VSI P VPPIVLAIAKSP +D YDLS
Sbjct: 248 LNSILLCGLRVGAAILIMQKFDIAPFLELIQKYKVSIGPFVPPIVLAIAKSPIVDSYDLS 307
Query: 304 SIRMIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPGAC 363
S+R + SG APLGKELED VR KFP A LGQGYGMTEAGPVL M LAFAKEPF +K GAC
Sbjct: 308 SVRTVMSGAAPLGKELEDAVRTKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFDIKSGAC 367
Query: 364 GTVVRNAEMKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDEDGWLHTGD 423
GTVVRNAEMKIVD + G SLPRN PGEICIRGDQIMKGYLN+PEAT TID++GWLHTGD
Sbjct: 368 GTVVRNAEMKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPEATTRTIDKEGWLHTGD 427
Query: 424 IGLIDEDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEVPV 483
IG IDEDDELFIVDRLKELIKYKGFQVAPAE+EALLL HP ISDAAVVPMKDEQAGEVPV
Sbjct: 428 IGFIDEDDELFIVDRLKELIKYKGFQVAPAEIEALLLNHPNISDAAVVPMKDEQAGEVPV 487
Query: 484 AFVVKLKNSEVTEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKLATDF 543
AFVV+ S +TEDE+K FISKQV+FYKR+ RVFF++ +PKSPSGKILRK+LRA+LA
Sbjct: 488 AFVVRSNGSAITEDEVKDFISKQVIFYKRVKRVFFVETVPKSPSGKILRKDLRARLAAGV 547
Query: 544 PN 545
PN
Sbjct: 548 PN 547
BLAST of Lag0031211 vs. ExPASy Swiss-Prot
Match:
P14912 (4-coumarate--CoA ligase 1 OS=Petroselinum crispum OX=4043 GN=4CL1 PE=2 SV=1)
HSP 1 Score: 799.3 bits (2063), Expect = 2.8e-230
Identity = 397/538 (73.79%), Postives = 460/538 (85.50%), Query Frame = 0
Query: 7 QKEFIFRSKLPDIYIPNHLPLHSYCLQDNKAKIGHRTCLVNGVTGESFTYDDVDLAARKV 66
+++ IFRSKLPDIYIP HLPLH+YC +N +K+G ++CL+NG TGE+FTY V+L +RKV
Sbjct: 8 KEDLIFRSKLPDIYIPKHLPLHTYCF-ENISKVGDKSCLINGATGETFTYSQVELLSRKV 67
Query: 67 ASGLNKLGIAKCDVIMLLLPNSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQAKGSKA 126
ASGLNKLGI + D IMLLLPNSPE+ FAFLGASYRGAI T ANPFFT+AE+ KQ K S+A
Sbjct: 68 ASGLNKLGIQQGDTIMLLLPNSPEYFFAFLGASYRGAISTMANPFFTSAEVIKQLKASQA 127
Query: 127 KLIVTQASYYEKVKDITEELPDVKIMTVDSSPDGCLSFADLIQADEGEVAAVEIHPDDVV 186
KLI+TQA Y +KVKD E +++I+ +D +P CL F+ L++ADE E+ V I+ DDVV
Sbjct: 128 KLIITQACYVDKVKDYAAE-KNIQIICIDDAPQDCLHFSKLMEADESEMPEVVINSDDVV 187
Query: 187 ALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFRNDDVILCVLPLFHIYSLNSV 246
ALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDG+NPNLY ++DV++C+LPLFHIYSLN+V
Sbjct: 188 ALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGDNPNLYMHSEDVMICILPLFHIYSLNAV 247
Query: 247 LLCGLRAGATILIMPKFEIGSLLKLVEKYRVSIAPIVPPIVLAIAKSPDLDKYDLSSIRM 306
L CGLRAG TILIM KF+I L+L++KY+V+I P VPPIVLAIAKSP +DKYDLSS+R
Sbjct: 248 LCCGLRAGVTILIMQKFDIVPFLELIQKYKVTIGPFVPPIVLAIAKSPVVDKYDLSSVRT 307
Query: 307 IKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPGACGTVV 366
+ SG APLGKELED VRAKFP A LGQGYGMTEAGPVL M LAFAKEP+ +K GACGTVV
Sbjct: 308 VMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPYEIKSGACGTVV 367
Query: 367 RNAEMKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDEDGWLHTGDIGLI 426
RNAEMKIVD E SLPRN GEICIRGDQIMKGYLN+PE+T TIDE+GWLHTGDIG I
Sbjct: 368 RNAEMKIVDPETNASLPRNQRGEICIRGDQIMKGYLNDPESTRTTIDEEGWLHTGDIGFI 427
Query: 427 DEDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEVPVAFVV 486
D+DDELFIVDRLKE+IKYKGFQVAPAELEALLLTHP ISDAAVVPM DE+AGEVPVAFVV
Sbjct: 428 DDDDELFIVDRLKEIIKYKGFQVAPAELEALLLTHPTISDAAVVPMIDEKAGEVPVAFVV 487
Query: 487 KLKNSEVTEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKLAT-DFP 544
+ TE+EIKQF+SKQVVFYKRI RVFF+DAIPKSPSGKILRK+LRA++A+ D P
Sbjct: 488 RTNGFTTTEEEIKQFVSKQVVFYKRIFRVFFVDAIPKSPSGKILRKDLRARIASGDLP 543
BLAST of Lag0031211 vs. ExPASy Swiss-Prot
Match:
P14913 (4-coumarate--CoA ligase 1 OS=Petroselinum crispum OX=4043 GN=4CL2 PE=2 SV=1)
HSP 1 Score: 798.5 bits (2061), Expect = 4.7e-230
Identity = 398/538 (73.98%), Postives = 458/538 (85.13%), Query Frame = 0
Query: 7 QKEFIFRSKLPDIYIPNHLPLHSYCLQDNKAKIGHRTCLVNGVTGESFTYDDVDLAARKV 66
+++ IFRSKLPDIYIP HLPLH+YC +N +K+G ++CL+NG TGE+FTY V+L +RKV
Sbjct: 8 KEDLIFRSKLPDIYIPKHLPLHTYCF-ENISKVGDKSCLINGATGETFTYSQVELLSRKV 67
Query: 67 ASGLNKLGIAKCDVIMLLLPNSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQAKGSKA 126
ASGLNKLGI + D IMLLLPNSPE+ FAFLGASYRGAI T ANPFFT+AE+ KQ K S A
Sbjct: 68 ASGLNKLGIQQGDTIMLLLPNSPEYFFAFLGASYRGAISTMANPFFTSAEVIKQLKASLA 127
Query: 127 KLIVTQASYYEKVKDITEELPDVKIMTVDSSPDGCLSFADLIQADEGEVAAVEIHPDDVV 186
KLI+TQA Y +KVKD E +++I+ +D +P CL F+ L++ADE E+ V I DDVV
Sbjct: 128 KLIITQACYVDKVKDYAAE-KNIQIICIDDAPQDCLHFSKLMEADESEMPEVVIDSDDVV 187
Query: 187 ALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFRNDDVILCVLPLFHIYSLNSV 246
ALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDG+NPNLY ++DV++C+LPLFHIYSLN+V
Sbjct: 188 ALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGDNPNLYMHSEDVMICILPLFHIYSLNAV 247
Query: 247 LLCGLRAGATILIMPKFEIGSLLKLVEKYRVSIAPIVPPIVLAIAKSPDLDKYDLSSIRM 306
L CGLRAG TILIM KF+I L+L++KY+V+I P VPPIVLAIAKSP +DKYDLSS+R
Sbjct: 248 LCCGLRAGVTILIMQKFDIVPFLELIQKYKVTIGPFVPPIVLAIAKSPVVDKYDLSSVRT 307
Query: 307 IKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPGACGTVV 366
+ SG APLGKELED VRAKFP A LGQGYGMTEAGPVL M LAFAKEP+ +K GACGTVV
Sbjct: 308 VMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPYEIKSGACGTVV 367
Query: 367 RNAEMKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDEDGWLHTGDIGLI 426
RNAEMKIVD E SLPRN GEICIRGDQIMKGYLN+PE+T TIDE+GWLHTGDIG I
Sbjct: 368 RNAEMKIVDPETNASLPRNQRGEICIRGDQIMKGYLNDPESTRTTIDEEGWLHTGDIGFI 427
Query: 427 DEDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEVPVAFVV 486
D+DDELFIVDRLKE+IKYKGFQVAPAELEALLLTHP ISDAAVVPM DE+AGEVPVAFVV
Sbjct: 428 DDDDELFIVDRLKEIIKYKGFQVAPAELEALLLTHPTISDAAVVPMIDEKAGEVPVAFVV 487
Query: 487 KLKNSEVTEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKLAT-DFP 544
+ TE+EIKQF+SKQVVFYKRI RVFF+DAIPKSPSGKILRK+LRAK+A+ D P
Sbjct: 488 RTNGFTTTEEEIKQFVSKQVVFYKRIFRVFFVDAIPKSPSGKILRKDLRAKIASGDLP 543
BLAST of Lag0031211 vs. ExPASy Swiss-Prot
Match:
M4ISH0 (4-coumarate--CoA ligase CCL1 OS=Humulus lupulus OX=3486 GN=CCL1 PE=1 SV=1)
HSP 1 Score: 798.1 bits (2060), Expect = 6.1e-230
Identity = 399/537 (74.30%), Postives = 456/537 (84.92%), Query Frame = 0
Query: 7 QKEFIFRSKLPDIYIPNHLPLHSYCLQDNKAKIGHRTCLVNGVTGESFTYDDVDLAARKV 66
Q+EFIFRSKLPDIYIPNHLPLHSYC +N ++ R CL+NG TGE TY DVDL +RKV
Sbjct: 10 QEEFIFRSKLPDIYIPNHLPLHSYCF-ENISQFKDRPCLINGATGEIITYADVDLTSRKV 69
Query: 67 ASGLNKLGIAKCDVIMLLLPNSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQAKGSKA 126
A+GL+KLGI + DVIMLLL NSPEFV+AFL ASY GAI+T ANPF+T AE+AKQA SK
Sbjct: 70 AAGLDKLGIKQGDVIMLLLQNSPEFVYAFLAASYIGAIITTANPFYTPAEVAKQAAASKT 129
Query: 127 KLIVTQASYYEKVKDITEELPDVKIMTVDSSP--DGCLSFADLIQADEGEVAAVEIHPDD 186
KL++T A Y +KVK+ T VK+M VD+ P CL F++L QADE E+ AV+IHPDD
Sbjct: 130 KLVITLAGYIDKVKEFTGGESGVKVMCVDAPPPESECLHFSELTQADETEIPAVKIHPDD 189
Query: 187 VVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFRNDDVILCVLPLFHIYSLN 246
VVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDG+NPNLYF +DVILCVLPLFHIYSLN
Sbjct: 190 VVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGDNPNLYFHQNDVILCVLPLFHIYSLN 249
Query: 247 SVLLCGLRAGATILIMPKFEIGSLLKLVEKYRVSIAPIVPPIVLAIAKSPDLDKYDLSSI 306
S+LLCGLR GA ILIM KFEI LL+L+EK++V+IAP VPPIVL++AK PDL +YDLSSI
Sbjct: 250 SILLCGLRVGAAILIMQKFEISKLLELIEKFKVTIAPFVPPIVLSVAKCPDLHRYDLSSI 309
Query: 307 RMIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPGACGT 366
R + SGGAP+GKELED V+ K P A LGQGYGMTEAGPVL+M LAFAKEPFP+K GACGT
Sbjct: 310 RTVMSGGAPMGKELEDAVKEKLPHAKLGQGYGMTEAGPVLSMCLAFAKEPFPIKSGACGT 369
Query: 367 VVRNAEMKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDEDGWLHTGDIG 426
VVRNAEMKIVD + G SLPRN GEICIRG QIMKGY+N+ EAT TIDE GWLHTGDIG
Sbjct: 370 VVRNAEMKIVDPDTGASLPRNQSGEICIRGKQIMKGYINDAEATKGTIDEGGWLHTGDIG 429
Query: 427 LIDEDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEVPVAF 486
ID DDELFIVDRLKELIKYKGFQVAPAELE++L++HP I+DAAVVPMKDE AGEVPVAF
Sbjct: 430 FIDNDDELFIVDRLKELIKYKGFQVAPAELESMLISHPNITDAAVVPMKDEAAGEVPVAF 489
Query: 487 VVKLKNSEVTEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKLATD 542
VV+ S++TE++IKQ+ISKQVVFYKRIN+ FFI+ IPK+PSGKILRK LRAKL T+
Sbjct: 490 VVRSNGSKITEEDIKQYISKQVVFYKRINKAFFIEEIPKNPSGKILRKILRAKLVTE 545
BLAST of Lag0031211 vs. ExPASy TrEMBL
Match:
A0A6J1F599 (4-coumarate--CoA ligase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111442288 PE=4 SV=1)
HSP 1 Score: 1000.0 bits (2584), Expect = 3.9e-288
Identity = 501/546 (91.76%), Postives = 524/546 (95.97%), Query Frame = 0
Query: 1 MAIETLQKEFIFRSKLPDIYIPNHLPLHSYCLQDNKAKIGHRTCLVNGVTGESFTYDDVD 60
MA+E LQ +FIFRSKLPDIYIPNHLPLHSYCL +N AKIGHRTCL+N VTGESFTY DVD
Sbjct: 1 MAVEALQNDFIFRSKLPDIYIPNHLPLHSYCLHENLAKIGHRTCLINSVTGESFTYHDVD 60
Query: 61 LAARKVASGLNKLGIAKCDVIMLLLPNSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQ 120
LAARKVASGLNKLGIA+ DVIML+L NSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQ
Sbjct: 61 LAARKVASGLNKLGIAQGDVIMLILQNSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQ 120
Query: 121 AKGSKAKLIVTQASYYEKVKDITEELPDVKIMTVDSSPDGCLSFADLIQADEGEVAAVEI 180
AKGS AKLIVTQ+SYYEKVK+ITEELPDVKIMTVDS PDGCLSFADLIQADE E+ VEI
Sbjct: 121 AKGSNAKLIVTQSSYYEKVKEITEELPDVKIMTVDSPPDGCLSFADLIQADEREMPTVEI 180
Query: 181 HPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFRNDDVILCVLPLFHI 240
+PDDVVALPYSSGTTGLPKGVMLTH+GLVTSVAQQVDGENPNLY+RN+DVILCVLPLFHI
Sbjct: 181 NPDDVVALPYSSGTTGLPKGVMLTHRGLVTSVAQQVDGENPNLYYRNEDVILCVLPLFHI 240
Query: 241 YSLNSVLLCGLRAGATILIMPKFEIGSLLKLVEKYRVSIAPIVPPIVLAIAKSPDLDKYD 300
YSLNSVLLCGLRAG TILIMPKFEIGSLL+LV+K++VSIAPIVPPIVLAIAKSPDLDKYD
Sbjct: 241 YSLNSVLLCGLRAGTTILIMPKFEIGSLLQLVQKFKVSIAPIVPPIVLAIAKSPDLDKYD 300
Query: 301 LSSIRMIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360
LSSIR+IK GGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG
Sbjct: 301 LSSIRVIKCGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360
Query: 361 ACGTVVRNAEMKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDEDGWLHT 420
ACGTVVRNAEMKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATID+DGWLHT
Sbjct: 361 ACGTVVRNAEMKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT 420
Query: 421 GDIGLIDEDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEV 480
GDIG ID+DDELFIVDRLKELIKYKGFQVAPAE+EALLLTHP ISDAAVVPMKDE+AGEV
Sbjct: 421 GDIGFIDDDDELFIVDRLKELIKYKGFQVAPAEVEALLLTHPAISDAAVVPMKDEEAGEV 480
Query: 481 PVAFVVKLKNSEVTEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKLAT 540
PVAFVV+LKNSEVTEDEIKQFISKQVVFYKRI R F IDAIPKSPSGKILRKELRAKLA
Sbjct: 481 PVAFVVRLKNSEVTEDEIKQFISKQVVFYKRIKRAFMIDAIPKSPSGKILRKELRAKLAA 540
Query: 541 DFPNSN 547
FPNSN
Sbjct: 541 GFPNSN 546
BLAST of Lag0031211 vs. ExPASy TrEMBL
Match:
A0A6J1J912 (4-coumarate--CoA ligase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111482411 PE=4 SV=1)
HSP 1 Score: 991.9 bits (2563), Expect = 1.1e-285
Identity = 499/546 (91.39%), Postives = 522/546 (95.60%), Query Frame = 0
Query: 1 MAIETLQKEFIFRSKLPDIYIPNHLPLHSYCLQDNKAKIGHRTCLVNGVTGESFTYDDVD 60
MA+E LQ +FIFRSKLPDIYIPNHLPLHSYCL +N AKIGHRTCL+N VT ESFTY DVD
Sbjct: 1 MAVEALQNDFIFRSKLPDIYIPNHLPLHSYCLHENLAKIGHRTCLINAVTAESFTYHDVD 60
Query: 61 LAARKVASGLNKLGIAKCDVIMLLLPNSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQ 120
LAARKVASGLNKLGIA+ DVI+LLL NSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQ
Sbjct: 61 LAARKVASGLNKLGIAQGDVILLLLQNSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQ 120
Query: 121 AKGSKAKLIVTQASYYEKVKDITEELPDVKIMTVDSSPDGCLSFADLIQADEGEVAAVEI 180
AKGS AKLIVTQ+SYYEKVK+ITEELPDVKIMTVDS PDGCLSFADLIQADE E+ VEI
Sbjct: 121 AKGSNAKLIVTQSSYYEKVKEITEELPDVKIMTVDSPPDGCLSFADLIQADEREMPTVEI 180
Query: 181 HPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFRNDDVILCVLPLFHI 240
+PDDVVALPYSSGTTGLPKGVMLTH+GLVTSVAQQVDGENPNLY+RN+DVILCVLPLFHI
Sbjct: 181 NPDDVVALPYSSGTTGLPKGVMLTHRGLVTSVAQQVDGENPNLYYRNEDVILCVLPLFHI 240
Query: 241 YSLNSVLLCGLRAGATILIMPKFEIGSLLKLVEKYRVSIAPIVPPIVLAIAKSPDLDKYD 300
YSLNSVLLCGLRAG TILIMPKFEIGSLL+LV+K++VSIAPIVPPIVLAIAKSPDLDKYD
Sbjct: 241 YSLNSVLLCGLRAGTTILIMPKFEIGSLLQLVQKFKVSIAPIVPPIVLAIAKSPDLDKYD 300
Query: 301 LSSIRMIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360
LSSIR+IK GGAPLGKELED+VRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG
Sbjct: 301 LSSIRVIKCGGAPLGKELEDSVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360
Query: 361 ACGTVVRNAEMKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDEDGWLHT 420
ACGTVVRNAEMKIVDTENG SLPRNTPGEICIRGDQIMKGYLNNPEATAATID+DGWLHT
Sbjct: 361 ACGTVVRNAEMKIVDTENGCSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT 420
Query: 421 GDIGLIDEDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEV 480
GDIG ID+DDELFIVDRLKELIKYKGFQVAPAELEALLLTHP ISDAAVVPMKDE+AGEV
Sbjct: 421 GDIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPAISDAAVVPMKDEEAGEV 480
Query: 481 PVAFVVKLKNSEVTEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKLAT 540
PVAFVV+LKNSEVTEDEIKQFISKQVVFYKRI R F IDAIPKSPSGKILRKELRAKLAT
Sbjct: 481 PVAFVVRLKNSEVTEDEIKQFISKQVVFYKRIKRAFMIDAIPKSPSGKILRKELRAKLAT 540
Query: 541 DFPNSN 547
FP SN
Sbjct: 541 GFPISN 546
BLAST of Lag0031211 vs. ExPASy TrEMBL
Match:
A0A6J1G9T7 (4-coumarate--CoA ligase 1-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111452267 PE=4 SV=1)
HSP 1 Score: 986.5 bits (2549), Expect = 4.5e-284
Identity = 501/543 (92.27%), Postives = 519/543 (95.58%), Query Frame = 0
Query: 1 MAIETLQKEFIFRSKLPDIYIPNHLPLHSYCLQDNKAKIGHRTCLVNGVTGESFTYDDVD 60
MA ET+ +FIFRSKLPDIYIP HLPLHSYCLQ+ A+IGHRTCL+N VTGESFTY DVD
Sbjct: 1 MATETVH-DFIFRSKLPDIYIPKHLPLHSYCLQEKMAEIGHRTCLINAVTGESFTYSDVD 60
Query: 61 LAARKVASGLNKLGIAKCDVIMLLLPNSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQ 120
LAARK ASGL KLGIAK DVIMLLLPNSPEFVFAFLGASY GAIMTAANPFFTAAEIAKQ
Sbjct: 61 LAARKAASGLYKLGIAKGDVIMLLLPNSPEFVFAFLGASYLGAIMTAANPFFTAAEIAKQ 120
Query: 121 AKGSKAKLIVTQASYYEKVKDITEELPDVKIMTVDSSPDGCLSFADLIQADEGEVAAVEI 180
AKGSKAKLI+TQ+SYYEKVK+ITE+L D KIMTVDS GCLSFADLIQ + ++ AVEI
Sbjct: 121 AKGSKAKLIITQSSYYEKVKEITEQLSDAKIMTVDSPSLGCLSFADLIQGGDHKIPAVEI 180
Query: 181 HPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFRNDDVILCVLPLFHI 240
PDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLY+RNDDVILCVLPLFHI
Sbjct: 181 SPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYYRNDDVILCVLPLFHI 240
Query: 241 YSLNSVLLCGLRAGATILIMPKFEIGSLLKLVEKYRVSIAPIVPPIVLAIAKSPDLDKYD 300
YSLNSVLLCGLRAGATILIMPKFEIGSLL+LVEKYRVSIAPIVPPIVLAIAKSPDL+KYD
Sbjct: 241 YSLNSVLLCGLRAGATILIMPKFEIGSLLQLVEKYRVSIAPIVPPIVLAIAKSPDLEKYD 300
Query: 301 LSSIRMIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360
LSSIRMIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG
Sbjct: 301 LSSIRMIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360
Query: 361 ACGTVVRNAEMKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDEDGWLHT 420
ACGTVVRNAE+KIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATID +GWLHT
Sbjct: 361 ACGTVVRNAEIKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDSEGWLHT 420
Query: 421 GDIGLIDEDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEV 480
GDIGLID+DDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEV
Sbjct: 421 GDIGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEV 480
Query: 481 PVAFVVKLKNSEVTEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKLAT 540
PVAFVVKLKNSEVTEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKLA
Sbjct: 481 PVAFVVKLKNSEVTEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKLAA 540
Query: 541 DFP 544
DFP
Sbjct: 541 DFP 542
BLAST of Lag0031211 vs. ExPASy TrEMBL
Match:
A0A6J1KCG9 (4-coumarate--CoA ligase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111493060 PE=4 SV=1)
HSP 1 Score: 984.6 bits (2544), Expect = 1.7e-283
Identity = 500/546 (91.58%), Postives = 519/546 (95.05%), Query Frame = 0
Query: 1 MAIETLQKEFIFRSKLPDIYIPNHLPLHSYCLQDNKAKIGHRTCLVNGVTGESFTYDDVD 60
MA ET+ + IFRSKLPDIYIP HLPLHSYCLQ+ A+IGHRTCL+N VTGESFTY DVD
Sbjct: 1 MANETVH-DIIFRSKLPDIYIPKHLPLHSYCLQEKMAEIGHRTCLINAVTGESFTYSDVD 60
Query: 61 LAARKVASGLNKLGIAKCDVIMLLLPNSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQ 120
LAARK ASGL KLGIAK DVIMLLLPNSPEFVFAFLGASY GAIMTAANPFFTAAEIAKQ
Sbjct: 61 LAARKAASGLYKLGIAKGDVIMLLLPNSPEFVFAFLGASYLGAIMTAANPFFTAAEIAKQ 120
Query: 121 AKGSKAKLIVTQASYYEKVKDITEELPDVKIMTVDSSPDGCLSFADLIQADEGEVAAVEI 180
AKGSKAKL +TQ+SYYEKVK+ITE+LPD KIMTVDS P GCLSFADLIQ + E+ AVEI
Sbjct: 121 AKGSKAKLTITQSSYYEKVKEITEQLPDAKIMTVDSPPLGCLSFADLIQGGDHEIPAVEI 180
Query: 181 HPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFRNDDVILCVLPLFHI 240
PDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLY+RNDDVILCVLPLFHI
Sbjct: 181 SPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYYRNDDVILCVLPLFHI 240
Query: 241 YSLNSVLLCGLRAGATILIMPKFEIGSLLKLVEKYRVSIAPIVPPIVLAIAKSPDLDKYD 300
YSLNSVLLCGLRAGATILIMPKFEIGSLL+LVEKYRVSIAPIVPPIVLAIAKSPDL+KYD
Sbjct: 241 YSLNSVLLCGLRAGATILIMPKFEIGSLLQLVEKYRVSIAPIVPPIVLAIAKSPDLEKYD 300
Query: 301 LSSIRMIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360
LSSIRMIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG
Sbjct: 301 LSSIRMIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360
Query: 361 ACGTVVRNAEMKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDEDGWLHT 420
ACGTVVRNAE+KIVDTENGGSLPRNTPGEICI+GDQIMKGYLNNPEATAATID +GWLHT
Sbjct: 361 ACGTVVRNAEIKIVDTENGGSLPRNTPGEICIKGDQIMKGYLNNPEATAATIDNEGWLHT 420
Query: 421 GDIGLIDEDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEV 480
GDIGLID+DDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEV
Sbjct: 421 GDIGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEV 480
Query: 481 PVAFVVKLKNSEVTEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKLAT 540
PVAFVVKLKNS+VTEDEIKQFISKQVVFYKRINRVFFI AIPKSPSGKILRKELRAKLA
Sbjct: 481 PVAFVVKLKNSKVTEDEIKQFISKQVVFYKRINRVFFIGAIPKSPSGKILRKELRAKLAA 540
Query: 541 DFPNSN 547
DFP N
Sbjct: 541 DFPIPN 545
BLAST of Lag0031211 vs. ExPASy TrEMBL
Match:
A0A5A7SWF8 (4-coumarate--CoA ligase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1199G00110 PE=4 SV=1)
HSP 1 Score: 983.8 bits (2542), Expect = 2.9e-283
Identity = 494/546 (90.48%), Postives = 520/546 (95.24%), Query Frame = 0
Query: 1 MAIETLQKEFIFRSKLPDIYIPNHLPLHSYCLQDNKAKIGHRTCLVNGVTGESFTYDDVD 60
M IET++ + IFRSKLPDIYIPNHLPLHSYCLQ+N AKIGHRTCL+NGVTGESFT++DVD
Sbjct: 1 MGIETVENDVIFRSKLPDIYIPNHLPLHSYCLQENAAKIGHRTCLINGVTGESFTFNDVD 60
Query: 61 LAARKVASGLNKLGIAKCDVIMLLLPNSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQ 120
L ARKVASGLNKLGI K DVIMLLLPNSPEFVFAFLGAS+ GAIMTAANPFFTAAEIAKQ
Sbjct: 61 LTARKVASGLNKLGITKRDVIMLLLPNSPEFVFAFLGASHLGAIMTAANPFFTAAEIAKQ 120
Query: 121 AKGSKAKLIVTQASYYEKVKDITEELPDVKIMTVDSSPDGCLSFADLIQADEGEVAAVEI 180
AKGSKAKLI+TQ+SYYEK+K+ITEELP+VKIMTVDS DGCL F DLI+ADE E+ VEI
Sbjct: 121 AKGSKAKLIITQSSYYEKLKEITEELPEVKIMTVDSPHDGCLWFGDLIKADEREIPRVEI 180
Query: 181 HPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFRNDDVILCVLPLFHI 240
PDDVVALPYSSGTTGLPKGVMLTHK LVTSVAQQVDGENPNLY+ N+DVILCVLPLFHI
Sbjct: 181 DPDDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYGNEDVILCVLPLFHI 240
Query: 241 YSLNSVLLCGLRAGATILIMPKFEIGSLLKLVEKYRVSIAPIVPPIVLAIAKSPDLDKYD 300
YSLNSVLLCGLRAG+TILIMPKFEIG LL+LVEKY V++APIVPPIVLAIAKSP+L+KYD
Sbjct: 241 YSLNSVLLCGLRAGSTILIMPKFEIGLLLQLVEKYGVTVAPIVPPIVLAIAKSPELEKYD 300
Query: 301 LSSIRMIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360
LSSIR+IKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG
Sbjct: 301 LSSIRVIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360
Query: 361 ACGTVVRNAEMKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDEDGWLHT 420
ACGTVVRNAEMKIVDTE G SLPRNTPGEICIRGDQIMKGYLNNPEATAATID+DGWLHT
Sbjct: 361 ACGTVVRNAEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT 420
Query: 421 GDIGLIDEDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEV 480
GDIG IDEDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEV
Sbjct: 421 GDIGFIDEDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEV 480
Query: 481 PVAFVVKLKNSEVTEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKLAT 540
PVAFVVKLKNSE TEDEIKQFISKQVVFYK+INRVFFIDAIPKSPSGKILRKELRAKLA
Sbjct: 481 PVAFVVKLKNSEATEDEIKQFISKQVVFYKKINRVFFIDAIPKSPSGKILRKELRAKLAA 540
Query: 541 DFPNSN 547
FPNSN
Sbjct: 541 AFPNSN 546
BLAST of Lag0031211 vs. TAIR 10
Match:
AT1G51680.1 (4-coumarate:CoA ligase 1 )
HSP 1 Score: 763.1 bits (1969), Expect = 1.6e-220
Identity = 385/539 (71.43%), Postives = 451/539 (83.67%), Query Frame = 0
Query: 9 EFIFRSKLPDIYIPNHLPLHSYCLQDNKAKIGHRTCLVNGVTGESFTYDDVDLAARKVAS 68
+ IFRSKLPDIYIPNHL LH Y Q N ++ + CL+NG TG +TY DV + +R++A+
Sbjct: 22 DVIFRSKLPDIYIPNHLSLHDYIFQ-NISEFATKPCLINGPTGHVYTYSDVHVISRQIAA 81
Query: 69 GLNKLGIAKCDVIMLLLPNSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQAKGSKAKL 128
+KLG+ + DV+MLLLPN PEFV +FL AS+RGA TAANPFFT AEIAKQAK S KL
Sbjct: 82 NFHKLGVNQNDVVMLLLPNCPEFVLSFLAASFRGATATAANPFFTPAEIAKQAKASNTKL 141
Query: 129 IVTQASYYEKVKDITEELPDVKIMTVDSS-----PDGCLSFADLIQA--DEGEVA-AVEI 188
I+T+A Y +K+K + + V I+ +D + P+GCL F +L Q+ + EV +VEI
Sbjct: 142 IITEARYVDKIKPLQND-DGVVIVCIDDNESVPIPEGCLRFTELTQSTTEASEVIDSVEI 201
Query: 189 HPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFRNDDVILCVLPLFHI 248
PDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYF +DDVILCVLP+FHI
Sbjct: 202 SPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHSDDVILCVLPMFHI 261
Query: 249 YSLNSVLLCGLRAGATILIMPKFEIGSLLKLVEKYRVSIAPIVPPIVLAIAKSPDLDKYD 308
Y+LNS++LCGLR GA ILIMPKFEI LL+L+++ +V++AP+VPPIVLAIAKS + +KYD
Sbjct: 262 YALNSIMLCGLRVGAAILIMPKFEINLLLELIQRCKVTVAPMVPPIVLAIAKSSETEKYD 321
Query: 309 LSSIRMIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 368
LSSIR++KSG APLGKELED V AKFP A LGQGYGMTEAGPVL M L FAKEPFP+K G
Sbjct: 322 LSSIRVVKSGAAPLGKELEDAVNAKFPNAKLGQGYGMTEAGPVLAMSLGFAKEPFPVKSG 381
Query: 369 ACGTVVRNAEMKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDEDGWLHT 428
ACGTVVRNAEMKIVD + G SL RN PGEICIRG QIMKGYLNNP ATA TID+DGWLHT
Sbjct: 382 ACGTVVRNAEMKIVDPDTGDSLSRNQPGEICIRGHQIMKGYLNNPAATAETIDKDGWLHT 441
Query: 429 GDIGLIDEDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEV 488
GDIGLID+DDELFIVDRLKELIKYKGFQVAPAELEALL+ HP I+D AVV MK+E AGEV
Sbjct: 442 GDIGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLIGHPDITDVAVVAMKEEAAGEV 501
Query: 489 PVAFVVKLKNSEVTEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKLA 540
PVAFVVK K+SE++ED++KQF+SKQVVFYKRIN+VFF ++IPK+PSGKILRK+LRAKLA
Sbjct: 502 PVAFVVKSKDSELSEDDVKQFVSKQVVFYKRINKVFFTESIPKAPSGKILRKDLRAKLA 558
BLAST of Lag0031211 vs. TAIR 10
Match:
AT3G21240.1 (4-coumarate:CoA ligase 2 )
HSP 1 Score: 762.3 bits (1967), Expect = 2.7e-220
Identity = 378/540 (70.00%), Postives = 456/540 (84.44%), Query Frame = 0
Query: 9 EFIFRSKLPDIYIPNHLPLHSYCLQDNKAKIGHRTCLVNGVTGESFTYDDVDLAARKVAS 68
+ IFRS+LPDIYIPNHLPLH Y + +N ++ + CL+NG TGE +TY DV + +RK+A+
Sbjct: 20 DVIFRSRLPDIYIPNHLPLHDY-IFENISEFAAKPCLINGPTGEVYTYADVHVTSRKLAA 79
Query: 69 GLNKLGIAKCDVIMLLLPNSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQAKGSKAKL 128
GL+ LG+ + DV+M+LLPNSPE V FL AS+ GAI T+ANPFFT AEI+KQAK S AKL
Sbjct: 80 GLHNLGVKQHDVVMILLPNSPEVVLTFLAASFIGAITTSANPFFTPAEISKQAKASAAKL 139
Query: 129 IVTQASYYEKVKDITEELPDVKIMTVDSS--PDGCLSFADLIQADEGEVAAV--EIHPDD 188
IVTQ+ Y +K+K++ + V I+T DS P+ CL F++L Q++E V ++ +I P+D
Sbjct: 140 IVTQSRYVDKIKNLQND--GVLIVTTDSDAIPENCLRFSELTQSEEPRVDSIPEKISPED 199
Query: 189 VVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFRNDDVILCVLPLFHIYSLN 248
VVALP+SSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYF DDVILCVLP+FHIY+LN
Sbjct: 200 VVALPFSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFNRDDVILCVLPMFHIYALN 259
Query: 249 SVLLCGLRAGATILIMPKFEIGSLLKLVEKYRVSIAPIVPPIVLAIAKSPDLDKYDLSSI 308
S++LC LR GATILIMPKFEI LL+ +++ +V++A +VPPIVLAIAKSP+ +KYDLSS+
Sbjct: 260 SIMLCSLRVGATILIMPKFEITLLLEQIQRCKVTVAMVVPPIVLAIAKSPETEKYDLSSV 319
Query: 309 RMIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPGACGT 368
RM+KSG APLGKELED + AKFP A LGQGYGMTEAGPVL M L FAKEPFP+K GACGT
Sbjct: 320 RMVKSGAAPLGKELEDAISAKFPNAKLGQGYGMTEAGPVLAMSLGFAKEPFPVKSGACGT 379
Query: 369 VVRNAEMKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDEDGWLHTGDIG 428
VVRNAEMKI+D + G SLPRN PGEICIRG+QIMKGYLN+P ATA+TID+DGWLHTGD+G
Sbjct: 380 VVRNAEMKILDPDTGDSLPRNKPGEICIRGNQIMKGYLNDPLATASTIDKDGWLHTGDVG 439
Query: 429 LIDEDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEVPVAF 488
ID+DDELFIVDRLKELIKYKGFQVAPAELE+LL+ HP I+D AVV MK+E AGEVPVAF
Sbjct: 440 FIDDDDELFIVDRLKELIKYKGFQVAPAELESLLIGHPEINDVAVVAMKEEDAGEVPVAF 499
Query: 489 VVKLKNSEVTEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKLATDFPN 545
VV+ K+S ++EDEIKQF+SKQVVFYKRIN+VFF D+IPK+PSGKILRK+LRA+LA N
Sbjct: 500 VVRSKDSNISEDEIKQFVSKQVVFYKRINKVFFTDSIPKAPSGKILRKDLRARLANGLMN 556
BLAST of Lag0031211 vs. TAIR 10
Match:
AT1G51680.3 (4-coumarate:CoA ligase 1 )
HSP 1 Score: 708.0 bits (1826), Expect = 5.9e-204
Identity = 358/506 (70.75%), Postives = 419/506 (82.81%), Query Frame = 0
Query: 9 EFIFRSKLPDIYIPNHLPLHSYCLQDNKAKIGHRTCLVNGVTGESFTYDDVDLAARKVAS 68
+ IFRSKLPDIYIPNHL LH Y Q N ++ + CL+NG TG +TY DV + +R++A+
Sbjct: 22 DVIFRSKLPDIYIPNHLSLHDYIFQ-NISEFATKPCLINGPTGHVYTYSDVHVISRQIAA 81
Query: 69 GLNKLGIAKCDVIMLLLPNSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQAKGSKAKL 128
+KLG+ + DV+MLLLPN PEFV +FL AS+RGA TAANPFFT AEIAKQAK S KL
Sbjct: 82 NFHKLGVNQNDVVMLLLPNCPEFVLSFLAASFRGATATAANPFFTPAEIAKQAKASNTKL 141
Query: 129 IVTQASYYEKVKDITEELPDVKIMTVDSS-----PDGCLSFADLIQA--DEGEVA-AVEI 188
I+T+A Y +K+K + + V I+ +D + P+GCL F +L Q+ + EV +VEI
Sbjct: 142 IITEARYVDKIKPLQND-DGVVIVCIDDNESVPIPEGCLRFTELTQSTTEASEVIDSVEI 201
Query: 189 HPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFRNDDVILCVLPLFHI 248
PDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYF +DDVILCVLP+FHI
Sbjct: 202 SPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHSDDVILCVLPMFHI 261
Query: 249 YSLNSVLLCGLRAGATILIMPKFEIGSLLKLVEKYRVSIAPIVPPIVLAIAKSPDLDKYD 308
Y+LNS++LCGLR GA ILIMPKFEI LL+L+++ +V++AP+VPPIVLAIAKS + +KYD
Sbjct: 262 YALNSIMLCGLRVGAAILIMPKFEINLLLELIQRCKVTVAPMVPPIVLAIAKSSETEKYD 321
Query: 309 LSSIRMIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 368
LSSIR++KSG APLGKELED V AKFP A LGQGYGMTEAGPVL M L FAKEPFP+K G
Sbjct: 322 LSSIRVVKSGAAPLGKELEDAVNAKFPNAKLGQGYGMTEAGPVLAMSLGFAKEPFPVKSG 381
Query: 369 ACGTVVRNAEMKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDEDGWLHT 428
ACGTVVRNAEMKIVD + G SL RN PGEICIRG QIMKGYLNNP ATA TID+DGWLHT
Sbjct: 382 ACGTVVRNAEMKIVDPDTGDSLSRNQPGEICIRGHQIMKGYLNNPAATAETIDKDGWLHT 441
Query: 429 GDIGLIDEDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEV 488
GDIGLID+DDELFIVDRLKELIKYKGFQVAPAELEALL+ HP I+D AVV MK+E AGEV
Sbjct: 442 GDIGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLIGHPDITDVAVVAMKEEAAGEV 501
Query: 489 PVAFVVKLKNSEVTEDEIKQFISKQV 507
PVAFVVK K+SE++ED++KQF+SKQV
Sbjct: 502 PVAFVVKSKDSELSEDDVKQFVSKQV 525
BLAST of Lag0031211 vs. TAIR 10
Match:
AT1G65060.1 (4-coumarate:CoA ligase 3 )
HSP 1 Score: 689.5 bits (1778), Expect = 2.2e-198
Identity = 340/530 (64.15%), Postives = 431/530 (81.32%), Query Frame = 0
Query: 11 IFRSKLPDIYIPNHLPLHSYCLQDNKAKIGHRTCLVNGVTGESFTYDDVDLAARKVASGL 70
IFRSKLPDI IPNHLPLH+YC + + + + CL+ G TG+S+TY + L R+VASGL
Sbjct: 34 IFRSKLPDIDIPNHLPLHTYCF-EKLSSVSDKPCLIVGSTGKSYTYGETHLICRRVASGL 93
Query: 71 NKLGIAKCDVIMLLLPNSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQAKGSKAKLIV 130
KLGI K DVIM+LL NS EFVF+F+GAS GA+ T ANPF+T+ E+ KQ K S AKLI+
Sbjct: 94 YKLGIRKGDVIMILLQNSAEFVFSFMGASMIGAVSTTANPFYTSQELYKQLKSSGAKLII 153
Query: 131 TQASYYEKVKDITEELPDVKIMTVDSSPDGCLSFADLIQADEGE--VAAVEIHPDDVVAL 190
T + Y +K+K++ E L I T + +P+ CL F+ LI DE V+I DD AL
Sbjct: 154 THSQYVDKLKNLGENL--TLITTDEPTPENCLPFSTLITDDETNPFQETVDIGGDDAAAL 213
Query: 191 PYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFRNDDVILCVLPLFHIYSLNSVLL 250
P+SSGTTGLPKGV+LTHK L+TSVAQQVDG+NPNLY +++DVILCVLPLFHIYSLNSVLL
Sbjct: 214 PFSSGTTGLPKGVVLTHKSLITSVAQQVDGDNPNLYLKSNDVILCVLPLFHIYSLNSVLL 273
Query: 251 CGLRAGATILIMPKFEIGSLLKLVEKYRVSIAPIVPPIVLAIAKSPDLDKYDLSSIRMIK 310
LR+GAT+L+M KFEIG+LL L++++RV+IA +VPP+V+A+AK+P ++ YDLSS+R +
Sbjct: 274 NSLRSGATVLLMHKFEIGALLDLIQRHRVTIAALVPPLVIALAKNPTVNSYDLSSVRFVL 333
Query: 311 SGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPGACGTVVRN 370
SG APLGKEL+D++R + P+A+LGQGYGMTEAGPVL+M L FAKEP P K G+CGTVVRN
Sbjct: 334 SGAAPLGKELQDSLRRRLPQAILGQGYGMTEAGPVLSMSLGFAKEPIPTKSGSCGTVVRN 393
Query: 371 AEMKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDEDGWLHTGDIGLIDE 430
AE+K+V E SL N PGEICIRG QIMK YLN+PEAT+ATIDE+GWLHTGDIG +DE
Sbjct: 394 AELKVVHLETRLSLGYNQPGEICIRGQQIMKEYLNDPEATSATIDEEGWLHTGDIGYVDE 453
Query: 431 DDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEVPVAFVVKL 490
DDE+FIVDRLKE+IK+KGFQV PAELE+LL+ H I+DAAVVP DE AGEVPVAFVV+
Sbjct: 454 DDEIFIVDRLKEVIKFKGFQVPPAELESLLINHHSIADAAVVPQNDEVAGEVPVAFVVRS 513
Query: 491 KNSEVTEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKL 539
+++TE+++K++++KQVVFYKR+++VFF+ +IPKSPSGKILRK+L+AKL
Sbjct: 514 NGNDITEEDVKEYVAKQVVFYKRLHKVFFVASIPKSPSGKILRKDLKAKL 560
BLAST of Lag0031211 vs. TAIR 10
Match:
AT3G21230.1 (4-coumarate:CoA ligase 5 )
HSP 1 Score: 669.8 bits (1727), Expect = 1.8e-192
Identity = 330/544 (60.66%), Postives = 422/544 (77.57%), Query Frame = 0
Query: 9 EFIFRSKLPDIYIPNHLPLHSYCLQ---DNKAKIGHRTCLVNGVTGESFTYDDVDLAARK 68
+FIFRSKLPDI+IPNHLPL Y Q + TC+++G TG TY DV R+
Sbjct: 26 DFIFRSKLPDIFIPNHLPLTDYVFQRFSGDGDGDSSTTCIIDGATGRILTYADVQTNMRR 85
Query: 69 VASGLNKLGIAKCDVIMLLLPNSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQAKGSK 128
+A+G+++LGI DV+MLLLPNSPEF +FL +Y GA+ T ANPF+T EIAKQAK S
Sbjct: 86 IAAGIHRLGIRHGDVVMLLLPNSPEFALSFLAVAYLGAVSTTANPFYTQPEIAKQAKASA 145
Query: 129 AKLIVTQASYYEKVKDITEELPDVKIMTVD---------SSPDGCLSFADLIQADEGEVA 188
AK+I+T+ +K+ ++ + V I+ +D SS DGC+SF +L QADE E+
Sbjct: 146 AKMIITKKCLVDKLTNLKND--GVLIVCLDDDGDNGVVSSSDDGCVSFTELTQADETELL 205
Query: 189 AVEIHPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFRNDDVILCVLP 248
+I P+D VA+PYSSGTTGLPKGVM+THKGLVTS+AQ+VDGENPNL F +DVILC LP
Sbjct: 206 KPKISPEDTVAMPYSSGTTGLPKGVMITHKGLVTSIAQKVDGENPNLNFTANDVILCFLP 265
Query: 249 LFHIYSLNSVLLCGLRAGATILIMPKFEIGSLLKLVEKYRVSIAPIVPPIVLAIAKSPDL 308
+FHIY+L++++L +R GA +LI+P+FE+ +++L+++Y+V++ P+ PP+VLA KSP+
Sbjct: 266 MFHIYALDALMLSAMRTGAALLIVPRFELNLVMELIQRYKVTVVPVAPPVVLAFIKSPET 325
Query: 309 DKYDLSSIRMIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFP 368
++YDLSS+R++ SG A L KELED VR KFP A+ GQGYGMTE+G V LAFAK PF
Sbjct: 326 ERYDLSSVRIMLSGAATLKKELEDAVRLKFPNAIFGQGYGMTESGTV-AKSLAFAKNPFK 385
Query: 369 MKPGACGTVVRNAEMKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDEDG 428
K GACGTV+RNAEMK+VDTE G SLPRN GEIC+RG Q+MKGYLN+PEATA TID+DG
Sbjct: 386 TKSGACGTVIRNAEMKVVDTETGISLPRNKSGEICVRGHQLMKGYLNDPEATARTIDKDG 445
Query: 429 WLHTGDIGLIDEDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQ 488
WLHTGDIG +D+DDE+FIVDRLKELIK+KG+QVAPAELEALL++HP I DAAVV MKDE
Sbjct: 446 WLHTGDIGFVDDDDEIFIVDRLKELIKFKGYQVAPAELEALLISHPSIDDAAVVAMKDEV 505
Query: 489 AGEVPVAFVVKLKNSEVTEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKELRA 541
A EVPVAFV + + S++TED++K +++KQVV YKRI VFFI+ IPK+ SGKILRK+LRA
Sbjct: 506 ADEVPVAFVARSQGSQLTEDDVKSYVNKQVVHYKRIKMVFFIEVIPKAVSGKILRKDLRA 565
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| KAG6581611.1 | 1.3e-288 | 92.12 | 4-coumarate--CoA ligase 1, partial [Cucurbita argyrosperma subsp. sororia] | [more] |
| XP_022935389.1 | 8.1e-288 | 91.76 | 4-coumarate--CoA ligase 1-like [Cucurbita moschata] | [more] |
| XP_023526871.1 | 5.3e-287 | 91.58 | 4-coumarate--CoA ligase 1-like isoform X1 [Cucurbita pepo subsp. pepo] | [more] |
| XP_023524772.1 | 5.8e-286 | 92.31 | 4-coumarate--CoA ligase 1-like [Cucurbita pepo subsp. pepo] | [more] |
| XP_022983944.1 | 2.2e-285 | 91.39 | 4-coumarate--CoA ligase 1-like [Cucurbita maxima] | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| O24146 | 7.8e-233 | 75.00 | 4-coumarate--CoA ligase 2 OS=Nicotiana tabacum OX=4097 GN=4CL2 PE=1 SV=1 | [more] |
| O24145 | 2.1e-230 | 73.25 | 4-coumarate--CoA ligase 1 OS=Nicotiana tabacum OX=4097 GN=4CL1 PE=2 SV=1 | [more] |
| P14912 | 2.8e-230 | 73.79 | 4-coumarate--CoA ligase 1 OS=Petroselinum crispum OX=4043 GN=4CL1 PE=2 SV=1 | [more] |
| P14913 | 4.7e-230 | 73.98 | 4-coumarate--CoA ligase 1 OS=Petroselinum crispum OX=4043 GN=4CL2 PE=2 SV=1 | [more] |
| M4ISH0 | 6.1e-230 | 74.30 | 4-coumarate--CoA ligase CCL1 OS=Humulus lupulus OX=3486 GN=CCL1 PE=1 SV=1 | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A6J1F599 | 3.9e-288 | 91.76 | 4-coumarate--CoA ligase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111442288 PE=... | [more] |
| A0A6J1J912 | 1.1e-285 | 91.39 | 4-coumarate--CoA ligase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111482411 PE=4 ... | [more] |
| A0A6J1G9T7 | 4.5e-284 | 92.27 | 4-coumarate--CoA ligase 1-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC11... | [more] |
| A0A6J1KCG9 | 1.7e-283 | 91.58 | 4-coumarate--CoA ligase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111493060 PE=4 ... | [more] |
| A0A5A7SWF8 | 2.9e-283 | 90.48 | 4-coumarate--CoA ligase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo... | [more] |
