Lag0031209 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0031209
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	Germin-like protein
Location	chr11: 5807253 .. 5808041 (-)
RNA-Seq Expression	Lag0031209
Synteny	Lag0031209

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAGAAACTATCCTCAGCTTCTTTAATTTTCCTTCTCCTGTTTGTGTTATTCTCTTGTCTTGGAATCTCAGTTGCAGATTCTGATAATTTCTTTGATACTTGTCCTGCAGCCACAAACAAAAGTGAGTTCATCAATGGCTTCCCTTGCAAAAACCCCACCATTGTTACTACTTCTGACTTCAACTCAGCAAAGTTAACGGGACCGGTCAATACCGACGATCGGTTTGGTTCGTTGGTGAAGCTTGTCACTGCTGCAGACTTCCCTGGTCTGAACACATTGGGGCTTTCCATTTCTAGAACTGATTTGGGCACTGATGCCATCGTTTCTCCTCACTCGCACCCGAGAGCCACCGAGATGTTCTTTGTGAATCAAGGTACGTATTCGGTTTGAGTGGTGAATTAACATGGTATCAGAGCTGGATGTTCTATGCTCGTCCTCCTCTCTTCTCATCTCTCTCGACAAATATGATTCTCAGGCATTGTTCTAGCTGGATTTATCGACACCAAGAACAGACTATTTCAGAATTTTCTAAAAGCAGGCGATGTGATTGTATTTCCAAAGGGGTTGTTGCACTTCTGTCTGAATTCTGGTTATGAGACTGCCACCATTTTCTCTGTGTATAATAGTCAGAATCCAGGAGTTGTGAACGTCAACGGCGCCATGTTCGAGCCAGCTGATTCGAGTCATTTCGTAGACAAGTTCATTGAGAGATTAAAAGCTCTTTCTTCACTGTCAGATCTCAAATTCAACCCAAATGTAACTCTTCCCAGATACATAAGTTTGTAA

mRNA sequence

ATGAAGAAACTATCCTCAGCTTCTTTAATTTTCCTTCTCCTGTTTGTGTTATTCTCTTGTCTTGGAATCTCAGTTGCAGATTCTGATAATTTCTTTGATACTTGTCCTGCAGCCACAAACAAAAGTGAGTTCATCAATGGCTTCCCTTGCAAAAACCCCACCATTGTTACTACTTCTGACTTCAACTCAGCAAAGTTAACGGGACCGGTCAATACCGACGATCGGTTTGGTTCGTTGGTGAAGCTTGTCACTGCTGCAGACTTCCCTGGTCTGAACACATTGGGGCTTTCCATTTCTAGAACTGATTTGGGCACTGATGCCATCGTTTCTCCTCACTCGCACCCGAGAGCCACCGAGATGTTCTTTGTGAATCAAGGCATTGTTCTAGCTGGATTTATCGACACCAAGAACAGACTATTTCAGAATTTTCTAAAAGCAGGCGATGTGATTGTATTTCCAAAGGGGTTGTTGCACTTCTGTCTGAATTCTGGTTATGAGACTGCCACCATTTTCTCTGTGTATAATAGTCAGAATCCAGGAGTTGTGAACGTCAACGGCGCCATGTTCGAGCCAGCTGATTCGAGTCATTTCGTAGACAAGTTCATTGAGAGATTAAAAGCTCTTTCTTCACTGTCAGATCTCAAATTCAACCCAAATGTAACTCTTCCCAGATACATAAGTTTGTAA

Coding sequence (CDS)

ATGAAGAAACTATCCTCAGCTTCTTTAATTTTCCTTCTCCTGTTTGTGTTATTCTCTTGTCTTGGAATCTCAGTTGCAGATTCTGATAATTTCTTTGATACTTGTCCTGCAGCCACAAACAAAAGTGAGTTCATCAATGGCTTCCCTTGCAAAAACCCCACCATTGTTACTACTTCTGACTTCAACTCAGCAAAGTTAACGGGACCGGTCAATACCGACGATCGGTTTGGTTCGTTGGTGAAGCTTGTCACTGCTGCAGACTTCCCTGGTCTGAACACATTGGGGCTTTCCATTTCTAGAACTGATTTGGGCACTGATGCCATCGTTTCTCCTCACTCGCACCCGAGAGCCACCGAGATGTTCTTTGTGAATCAAGGCATTGTTCTAGCTGGATTTATCGACACCAAGAACAGACTATTTCAGAATTTTCTAAAAGCAGGCGATGTGATTGTATTTCCAAAGGGGTTGTTGCACTTCTGTCTGAATTCTGGTTATGAGACTGCCACCATTTTCTCTGTGTATAATAGTCAGAATCCAGGAGTTGTGAACGTCAACGGCGCCATGTTCGAGCCAGCTGATTCGAGTCATTTCGTAGACAAGTTCATTGAGAGATTAAAAGCTCTTTCTTCACTGTCAGATCTCAAATTCAACCCAAATGTAACTCTTCCCAGATACATAAGTTTGTAA

Protein sequence

MKKLSSASLIFLLLFVLFSCLGISVADSDNFFDTCPAATNKSEFINGFPCKNPTIVTTSDFNSAKLTGPVNTDDRFGSLVKLVTAADFPGLNTLGLSISRTDLGTDAIVSPHSHPRATEMFFVNQGIVLAGFIDTKNRLFQNFLKAGDVIVFPKGLLHFCLNSGYETATIFSVYNSQNPGVVNVNGAMFEPADSSHFVDKFIERLKALSSLSDLKFNPNVTLPRYISL

Homology

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BLAST of Lag0031209 vs. NCBI nr
Match: XP_023522282.1 (germin-like protein subfamily 3 member 4 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 398.7 bits (1023), Expect = 3.5e-107
Identity = 197/223 (88.34%), Postives = 211/223 (94.62%), Query Frame = 0


Query: 6   SASLIFLLLFVLFSCLGISVADSDNFFDTCPAATNKSEFINGFPCKNPTIVTTSDFNSAK 65
           S+SLIFLL+ VLFSCLGISVADS N FD+CP AT++ +FINGFPCKNPTIV T DF SAK
Sbjct: 19  SSSLIFLLV-VLFSCLGISVADSHNVFDSCPTATDRIDFINGFPCKNPTIVRTDDFTSAK 78

Query: 66  LTGPVNTDDRFGSLVKLVTAADFPGLNTLGLSISRTDLGTDAIVSPHSHPRATEMFFVNQ 125
           LTGPVNTDDRF SLVK+VTAADFPGLN+LGLSISRTDLGTDAI+SPHSHPRATEMFFVNQ
Sbjct: 79  LTGPVNTDDRFSSLVKVVTAADFPGLNSLGLSISRTDLGTDAIISPHSHPRATEMFFVNQ 138

Query: 126 GIVLAGFIDTKNRLFQNFLKAGDVIVFPKGLLHFCLNSGYETATIFSVYNSQNPGVVNVN 185
           GIVLAGFIDTKNRLFQ FLK GDVIVFPKGLLHFCLNSGYETATIFSVYNSQNPGVVNVN
Sbjct: 139 GIVLAGFIDTKNRLFQEFLKPGDVIVFPKGLLHFCLNSGYETATIFSVYNSQNPGVVNVN 198

Query: 186 GAMFEPADSSHFVDKFIERLKALSSLSDLKFNPNVTLPRYISL 229
           GAMFEPADSSHF+DKF+++LKALSSL +LKFNPNVTLPRYISL
Sbjct: 199 GAMFEPADSSHFIDKFLDKLKALSSLPNLKFNPNVTLPRYISL 240

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BLAST of Lag0031209 vs. NCBI nr
Match: XP_022153439.1 (germin-like protein subfamily 3 member 4 [Momordica charantia])

HSP 1 Score: 398.7 bits (1023), Expect = 3.5e-107
Identity = 198/228 (86.84%), Postives = 214/228 (93.86%), Query Frame = 0


Query: 1   MKKLSSASLIFLLLFVLFSCLGISVADSDNFFDTCPAATNKSEFINGFPCKNPTIVTTSD 60
           MKK SS+SLIFLLLFVLFSCLGISVADS+N FDTCP ATNKSEFINGFPCKNPTIVTTSD
Sbjct: 1   MKKPSSSSLIFLLLFVLFSCLGISVADSNNVFDTCPTATNKSEFINGFPCKNPTIVTTSD 60

Query: 61  FNSAKLTGPVNTDDRFGSLVKLVTAADFPGLNTLGLSISRTDLGTDAIVSPHSHPRATEM 120
           FNSAKLTGPV+TDD+F S V ++TAA+F GLNTLGLSISRTDLGTDAIVSPHSHPRATE+
Sbjct: 61  FNSAKLTGPVDTDDQFISSVNILTAAEFSGLNTLGLSISRTDLGTDAIVSPHSHPRATEI 120

Query: 121 FFVNQGIVLAGFIDTKNRLFQNFLKAGDVIVFPKGLLHFCLNSGYETATIFSVYNSQNPG 180
           FFVNQGIVLAGF+DTKNRLFQ+FLKAGDV+VFPKGLLHFCLNSGYE A IFSV+NSQNPG
Sbjct: 121 FFVNQGIVLAGFLDTKNRLFQSFLKAGDVMVFPKGLLHFCLNSGYEPAIIFSVFNSQNPG 180

Query: 181 VVNVNGAMFEPADSSHFVDKFIERLKALSSLSDLKFNPNVTLPRYISL 229
           VVN+NGAMFEPADS+H V KF+ERLK+LSSLS LKFNPNVTL  YISL
Sbjct: 181 VVNINGAMFEPADSNHLVHKFLERLKSLSSLSSLKFNPNVTLTTYISL 228

[Close]

BLAST of Lag0031209 vs. NCBI nr
Match: KAG6606852.1 (Germin-like protein subfamily 3 member 4, partial [Cucurbita argyrosperma subsp. sororia] >KAG7036559.1 Germin-like protein subfamily 3 member 4, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 397.9 bits (1021), Expect = 5.9e-107
Identity = 197/222 (88.74%), Postives = 209/222 (94.14%), Query Frame = 0


Query: 7   ASLIFLLLFVLFSCLGISVADSDNFFDTCPAATNKSEFINGFPCKNPTIVTTSDFNSAKL 66
           +SLIFLL+ V FSCLGISVADS N FD+CP AT++ +FINGFPCKNPTIV T DF SAKL
Sbjct: 6   SSLIFLLV-VFFSCLGISVADSHNVFDSCPTATDRIDFINGFPCKNPTIVRTDDFTSAKL 65

Query: 67  TGPVNTDDRFGSLVKLVTAADFPGLNTLGLSISRTDLGTDAIVSPHSHPRATEMFFVNQG 126
           TGPVNTDDRF SLVK+VTAADFPGLN+LGLSISRTDLGTDAIVSPHSHPRATEMFFVNQG
Sbjct: 66  TGPVNTDDRFSSLVKVVTAADFPGLNSLGLSISRTDLGTDAIVSPHSHPRATEMFFVNQG 125

Query: 127 IVLAGFIDTKNRLFQNFLKAGDVIVFPKGLLHFCLNSGYETATIFSVYNSQNPGVVNVNG 186
           IVLAGFIDTKNRLFQ FLK GDVIVFPKGLLHFCLNSGYETATIFSVYNSQNPGVVNVNG
Sbjct: 126 IVLAGFIDTKNRLFQEFLKPGDVIVFPKGLLHFCLNSGYETATIFSVYNSQNPGVVNVNG 185

Query: 187 AMFEPADSSHFVDKFIERLKALSSLSDLKFNPNVTLPRYISL 229
           AMFEPADSSHF+DKF+++LKALSSL DLKFNPNVTLPRYISL
Sbjct: 186 AMFEPADSSHFIDKFLDKLKALSSLPDLKFNPNVTLPRYISL 226

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BLAST of Lag0031209 vs. NCBI nr
Match: XP_022948659.1 (germin-like protein subfamily 3 member 4 [Cucurbita moschata] >XP_022948660.1 germin-like protein subfamily 3 member 4 [Cucurbita moschata])

HSP 1 Score: 396.0 bits (1016), Expect = 2.2e-106
Identity = 196/223 (87.89%), Postives = 210/223 (94.17%), Query Frame = 0


Query: 6   SASLIFLLLFVLFSCLGISVADSDNFFDTCPAATNKSEFINGFPCKNPTIVTTSDFNSAK 65
           S+SLIFLL+ V FSCLGISVADS N FD+CP AT++ +FINGFPCKNPTIV T DF SAK
Sbjct: 5   SSSLIFLLV-VFFSCLGISVADSHNVFDSCPTATDRIDFINGFPCKNPTIVRTDDFTSAK 64

Query: 66  LTGPVNTDDRFGSLVKLVTAADFPGLNTLGLSISRTDLGTDAIVSPHSHPRATEMFFVNQ 125
           LTGPVNTDDRF SLVK+VTAADFPGLN+LGLSISRTDLGTDAIVSPHSHPRATEMFFVNQ
Sbjct: 65  LTGPVNTDDRFSSLVKVVTAADFPGLNSLGLSISRTDLGTDAIVSPHSHPRATEMFFVNQ 124

Query: 126 GIVLAGFIDTKNRLFQNFLKAGDVIVFPKGLLHFCLNSGYETATIFSVYNSQNPGVVNVN 185
           GIVLAGFIDTKNRLFQ FLK GDVIVFPKGLLHFCLNSGYETATIFSVYNSQNPGVVNVN
Sbjct: 125 GIVLAGFIDTKNRLFQEFLKPGDVIVFPKGLLHFCLNSGYETATIFSVYNSQNPGVVNVN 184

Query: 186 GAMFEPADSSHFVDKFIERLKALSSLSDLKFNPNVTLPRYISL 229
           GAMF+PADSSHF+DKF+++LKALSSL +LKFNPNVTLPRYISL
Sbjct: 185 GAMFKPADSSHFIDKFLDKLKALSSLPNLKFNPNVTLPRYISL 226

[Close]

BLAST of Lag0031209 vs. NCBI nr
Match: XP_022998490.1 (germin-like protein subfamily 3 member 4 [Cucurbita maxima])

HSP 1 Score: 394.4 bits (1012), Expect = 6.5e-106
Identity = 194/223 (87.00%), Postives = 209/223 (93.72%), Query Frame = 0


Query: 6   SASLIFLLLFVLFSCLGISVADSDNFFDTCPAATNKSEFINGFPCKNPTIVTTSDFNSAK 65
           S+SLIFLLL V FSCLGI+VADS N FD+CP AT++ +F+NGFPCKNPT+V T DF SAK
Sbjct: 5   SSSLIFLLL-VFFSCLGIAVADSHNVFDSCPTATDRIDFVNGFPCKNPTVVRTDDFTSAK 64

Query: 66  LTGPVNTDDRFGSLVKLVTAADFPGLNTLGLSISRTDLGTDAIVSPHSHPRATEMFFVNQ 125
           LTGPVNTDDRF SLVK+VTAADFPGLN+LGLSISRTDLGTDAIVSPHSHPRATEMFFVNQ
Sbjct: 65  LTGPVNTDDRFSSLVKVVTAADFPGLNSLGLSISRTDLGTDAIVSPHSHPRATEMFFVNQ 124

Query: 126 GIVLAGFIDTKNRLFQNFLKAGDVIVFPKGLLHFCLNSGYETATIFSVYNSQNPGVVNVN 185
           GIVLAGFIDTKNRLFQ FLK GDVIVFPKGLLHFCLNSGYETATIFSVYNSQNPGVVNVN
Sbjct: 125 GIVLAGFIDTKNRLFQEFLKPGDVIVFPKGLLHFCLNSGYETATIFSVYNSQNPGVVNVN 184

Query: 186 GAMFEPADSSHFVDKFIERLKALSSLSDLKFNPNVTLPRYISL 229
           GAMFEPADSSHF+D F+++LKALSSL +LKFNPNVTLPRYISL
Sbjct: 185 GAMFEPADSSHFIDMFLDKLKALSSLPNLKFNPNVTLPRYISL 226

[Close]

BLAST of Lag0031209 vs. ExPASy Swiss-Prot
Match: Q9FLT3 (Germin-like protein subfamily 3 member 4 OS=Arabidopsis thaliana OX=3702 GN=At5g61750 PE=2 SV=1)

HSP 1 Score: 209.9 bits (533), Expect = 3.0e-53
Identity = 103/172 (59.88%), Postives = 127/172 (73.84%), Query Frame = 0


Query: 16  VLFSCLGISVA-DSDNFFDTCPAATNKSE--FINGFPCKNPTIVTTSDFNSAKLTGPVNT 75
           ++F  + +SV+ DSDN  DTCP A  +    FING+PCKNPT +T  DF S KLT   +T
Sbjct: 7   IVFCAIFLSVSGDSDNMQDTCPTAPGEQSIFFINGYPCKNPTKITAQDFKSTKLTEAGDT 66

Query: 76  DDRFGSLVKLVTAADFPGLNTLGLSISRTDLGTDAIVSPHSHPRATEMFFVNQGIVLAGF 135
           D+   S V L+TA +FPGLNTLGLS+SRTDL  D  V  HSHPR++EM FV +G+V AGF
Sbjct: 67  DNYLQSNVTLLTALEFPGLNTLGLSVSRTDLERDGSVPFHSHPRSSEMLFVVKGVVFAGF 126

Query: 136 IDTKNRLFQNFLKAGDVIVFPKGLLHFCLNSGYETATIFSVYNSQNPGVVNV 185
           +DT N++FQ  L+ GDV VFPKGLLHFCL+ G+E AT FS YNSQNPGVVN+
Sbjct: 127 VDTNNKIFQTVLQKGDVFVFPKGLLHFCLSGGFEPATAFSFYNSQNPGVVNI 178

[Close]

BLAST of Lag0031209 vs. ExPASy Swiss-Prot
Match: Q2R352 (Germin-like protein 11-1 OS=Oryza sativa subsp. japonica OX=39947 GN=Os11g0537350 PE=2 SV=1)

HSP 1 Score: 180.3 bits (456), Expect = 2.5e-44
Identity = 95/191 (49.74%), Postives = 123/191 (64.40%), Query Frame = 0


Query: 3   KLSSASLIFLLLFVLFSCLGISVADSDNFFDTCPAAT--NKSEFINGFPCKNPTIVTTSD 62
           KLS+    +LLL  LF+   IS  DS    D CP A    +  F+NGF CK+P+ +  SD
Sbjct: 2   KLSTVLCCYLLLLGLFAPEIIS--DSPPLQDVCPMAPQGERKLFMNGFFCKSPSTIMASD 61

Query: 63  FNSAKLTGPVNTDDRFGSLVKLVTAADFPGLNTLGLSISRTDLGTDAIVSPHSHPRATEM 122
           F +  L    + D+   S   ++TA +FPGLNTLG+S++RTD+     V PHSHPRA+EM
Sbjct: 62  FKTLLLNHAGDLDNMVRSSANIITATEFPGLNTLGISMARTDIAVSGAVLPHSHPRASEM 121

Query: 123 FFVNQGIVLAGFIDTKNRLFQNFLKAGDVIVFPKGLLHFCLNSGYETATIFSVYNSQNPG 182
            FV+ G V+AGF DTK +LFQ  L  GDV +FP+GL+HF +N G+  AT FSV NSQNPG
Sbjct: 122 MFVHSGSVVAGFFDTKGKLFQKTLAEGDVFIFPRGLVHFIMNYGFGLATTFSVLNSQNPG 181

Query: 183 VVNVNGAMFEP 192
           VV +  AMF P
Sbjct: 182 VVGITHAMFAP 190

[Close]

BLAST of Lag0031209 vs. ExPASy Swiss-Prot
Match: P94014 (Germin-like protein subfamily 2 member 1 OS=Arabidopsis thaliana OX=3702 GN=GLP4 PE=2 SV=2)

HSP 1 Score: 147.1 bits (370), Expect = 2.4e-34
Identity = 80/186 (43.01%), Postives = 107/186 (57.53%), Query Frame = 0


Query: 4   LSSASLIFLLLFVLFSCLGISVADSDNFFDTCPAATNKSEFINGFPCKNPTIVTTSDFNS 63
           ++S +L  LLL    S    S AD D   D C A       INGFPCK+   VT++DF S
Sbjct: 1   MASPTLTLLLLLTTVSFFISSSADPDMLQDLCVADLPSGIKINGFPCKDAATVTSADFFS 60

Query: 64  AKLTGPVNTDDRFGSLVKLVTAADFPGLNTLGLSISRTDLGTDAIVSPHSHPRATEMFFV 123
             L  P  T++ FG+LV        PGLNTLG+S+SR D     +  PH+HPRATE+ FV
Sbjct: 61  QGLAKPGLTNNTFGALVTGANVMTIPGLNTLGVSLSRIDYAPGGLNPPHTHPRATEVVFV 120

Query: 124 NQGIVLAGFIDTKNRLFQNFLKAGDVIVFPKGLLHFCLNSGYETATIFSVYNSQNPGVVN 183
            +G +  GF+ T N+L    LK GDV  FPKGL+HF  N+G   A++ + +NSQ PG  +
Sbjct: 121 LEGTLDVGFLTTANKLISQSLKKGDVFAFPKGLVHFQKNNGDVPASVIAAFNSQLPGTQS 180

Query: 184 VNGAMF 190
           +   +F
Sbjct: 181 LGATLF 186

[Close]

BLAST of Lag0031209 vs. ExPASy Swiss-Prot
Match: Q9S8P4 (Rhicadhesin receptor OS=Pisum sativum OX=3888 GN=GER1 PE=1 SV=2)

HSP 1 Score: 142.5 bits (358), Expect = 5.9e-33
Identity = 78/178 (43.82%), Postives = 103/178 (57.87%), Query Frame = 0


Query: 12  LLLFVLFSCLGISVADSDNFFDTCPAATNKSEFINGFPCKNPTIVTTSDFNSAKLTGPVN 71
           LLL VL +    + AD+D   D C A       +NGF CK  + VT  DF S  L     
Sbjct: 7   LLLVVLATATTATAADADALQDLCVADYASVILVNGFACKPASNVTAEDFFSNLLVKQGA 66

Query: 72  TDDRFGSLVKLVTAADFPGLNTLGLSISRTDLGTDAIVSPHSHPRATEMFFVNQGIVLAG 131
           T++ FGSLV        PGLNTLG+S++R D     +  PH+HPRATEM FV +G +  G
Sbjct: 67  TNNTFGSLVTGANVQRIPGLNTLGVSMARIDYAPGGLNPPHTHPRATEMVFVLEGQLDVG 126

Query: 132 FIDTKNRLFQNFLKAGDVIVFPKGLLHFCLNSGYETATIFSVYNSQNPGVVNVNGAMF 190
           FI T N+L    +  G+  VFPKGL+HF  N+G+E AT+ + +NSQ PG VN+   +F
Sbjct: 127 FITTTNQLIAKTIAKGETFVFPKGLVHFQKNNGWEPATVIAGFNSQLPGTVNIPLTLF 184

[Close]

BLAST of Lag0031209 vs. ExPASy Swiss-Prot
Match: P45852 (Germin-like protein OS=Mesembryanthemum crystallinum OX=3544 PE=2 SV=1)

HSP 1 Score: 139.8 bits (351), Expect = 3.8e-32
Identity = 73/164 (44.51%), Postives = 93/164 (56.71%), Query Frame = 0


Query: 40  NKSEFINGFPCKNPTIVTTSDFNSAKLTGPVNTDDRFGSLVKLVTAADFPGLNTLGLSIS 99
           N   F+NG  CK+P  V   DF    L  P NTD+  G    LVTAA+ PGLNTLG+S++
Sbjct: 39  NDGLFVNGLFCKDPMEVNPDDFLFRGLNMPANTDNALGFAATLVTAANLPGLNTLGISVA 98

Query: 100 RTDLGTDAIVSPHSHPRATEMFFVNQGIVLAGFI-----DTKNRLFQNFLKAGDVIVFPK 159
           R D     +  PH+HPRATE+F V +G    GF+     D  N+LF   L  GDV VFP+
Sbjct: 99  RLDFAPHGLNPPHTHPRATEVFVVLEGTFYVGFVTSNLADGGNKLFAKVLNKGDVFVFPQ 158

Query: 160 GLLHFCLNSGYETATIFSVYNSQNPGVVNVNGAMFEPADSSHFV 199
           GL+HF LN G       S  +SQNPGV+ +  A+F P   S  +
Sbjct: 159 GLIHFQLNIGNYPGVGISGLSSQNPGVITIANAVFGPEHLSQLM 202

[Close]

BLAST of Lag0031209 vs. ExPASy TrEMBL
Match: A0A6J1DJ19 (Germin-like protein OS=Momordica charantia OX=3673 GN=LOC111020943 PE=3 SV=1)

HSP 1 Score: 398.7 bits (1023), Expect = 1.7e-107
Identity = 198/228 (86.84%), Postives = 214/228 (93.86%), Query Frame = 0


Query: 1   MKKLSSASLIFLLLFVLFSCLGISVADSDNFFDTCPAATNKSEFINGFPCKNPTIVTTSD 60
           MKK SS+SLIFLLLFVLFSCLGISVADS+N FDTCP ATNKSEFINGFPCKNPTIVTTSD
Sbjct: 1   MKKPSSSSLIFLLLFVLFSCLGISVADSNNVFDTCPTATNKSEFINGFPCKNPTIVTTSD 60

Query: 61  FNSAKLTGPVNTDDRFGSLVKLVTAADFPGLNTLGLSISRTDLGTDAIVSPHSHPRATEM 120
           FNSAKLTGPV+TDD+F S V ++TAA+F GLNTLGLSISRTDLGTDAIVSPHSHPRATE+
Sbjct: 61  FNSAKLTGPVDTDDQFISSVNILTAAEFSGLNTLGLSISRTDLGTDAIVSPHSHPRATEI 120

Query: 121 FFVNQGIVLAGFIDTKNRLFQNFLKAGDVIVFPKGLLHFCLNSGYETATIFSVYNSQNPG 180
           FFVNQGIVLAGF+DTKNRLFQ+FLKAGDV+VFPKGLLHFCLNSGYE A IFSV+NSQNPG
Sbjct: 121 FFVNQGIVLAGFLDTKNRLFQSFLKAGDVMVFPKGLLHFCLNSGYEPAIIFSVFNSQNPG 180

Query: 181 VVNVNGAMFEPADSSHFVDKFIERLKALSSLSDLKFNPNVTLPRYISL 229
           VVN+NGAMFEPADS+H V KF+ERLK+LSSLS LKFNPNVTL  YISL
Sbjct: 181 VVNINGAMFEPADSNHLVHKFLERLKSLSSLSSLKFNPNVTLTTYISL 228

[Close]

BLAST of Lag0031209 vs. ExPASy TrEMBL
Match: A0A6J1GAI2 (Germin-like protein OS=Cucurbita moschata OX=3662 GN=LOC111452269 PE=3 SV=1)

HSP 1 Score: 396.0 bits (1016), Expect = 1.1e-106
Identity = 196/223 (87.89%), Postives = 210/223 (94.17%), Query Frame = 0


Query: 6   SASLIFLLLFVLFSCLGISVADSDNFFDTCPAATNKSEFINGFPCKNPTIVTTSDFNSAK 65
           S+SLIFLL+ V FSCLGISVADS N FD+CP AT++ +FINGFPCKNPTIV T DF SAK
Sbjct: 5   SSSLIFLLV-VFFSCLGISVADSHNVFDSCPTATDRIDFINGFPCKNPTIVRTDDFTSAK 64

Query: 66  LTGPVNTDDRFGSLVKLVTAADFPGLNTLGLSISRTDLGTDAIVSPHSHPRATEMFFVNQ 125
           LTGPVNTDDRF SLVK+VTAADFPGLN+LGLSISRTDLGTDAIVSPHSHPRATEMFFVNQ
Sbjct: 65  LTGPVNTDDRFSSLVKVVTAADFPGLNSLGLSISRTDLGTDAIVSPHSHPRATEMFFVNQ 124

Query: 126 GIVLAGFIDTKNRLFQNFLKAGDVIVFPKGLLHFCLNSGYETATIFSVYNSQNPGVVNVN 185
           GIVLAGFIDTKNRLFQ FLK GDVIVFPKGLLHFCLNSGYETATIFSVYNSQNPGVVNVN
Sbjct: 125 GIVLAGFIDTKNRLFQEFLKPGDVIVFPKGLLHFCLNSGYETATIFSVYNSQNPGVVNVN 184

Query: 186 GAMFEPADSSHFVDKFIERLKALSSLSDLKFNPNVTLPRYISL 229
           GAMF+PADSSHF+DKF+++LKALSSL +LKFNPNVTLPRYISL
Sbjct: 185 GAMFKPADSSHFIDKFLDKLKALSSLPNLKFNPNVTLPRYISL 226

[Close]

BLAST of Lag0031209 vs. ExPASy TrEMBL
Match: A0A6J1KCN7 (Germin-like protein OS=Cucurbita maxima OX=3661 GN=LOC111493105 PE=3 SV=1)

HSP 1 Score: 394.4 bits (1012), Expect = 3.2e-106
Identity = 194/223 (87.00%), Postives = 209/223 (93.72%), Query Frame = 0


Query: 6   SASLIFLLLFVLFSCLGISVADSDNFFDTCPAATNKSEFINGFPCKNPTIVTTSDFNSAK 65
           S+SLIFLLL V FSCLGI+VADS N FD+CP AT++ +F+NGFPCKNPT+V T DF SAK
Sbjct: 5   SSSLIFLLL-VFFSCLGIAVADSHNVFDSCPTATDRIDFVNGFPCKNPTVVRTDDFTSAK 64

Query: 66  LTGPVNTDDRFGSLVKLVTAADFPGLNTLGLSISRTDLGTDAIVSPHSHPRATEMFFVNQ 125
           LTGPVNTDDRF SLVK+VTAADFPGLN+LGLSISRTDLGTDAIVSPHSHPRATEMFFVNQ
Sbjct: 65  LTGPVNTDDRFSSLVKVVTAADFPGLNSLGLSISRTDLGTDAIVSPHSHPRATEMFFVNQ 124

Query: 126 GIVLAGFIDTKNRLFQNFLKAGDVIVFPKGLLHFCLNSGYETATIFSVYNSQNPGVVNVN 185
           GIVLAGFIDTKNRLFQ FLK GDVIVFPKGLLHFCLNSGYETATIFSVYNSQNPGVVNVN
Sbjct: 125 GIVLAGFIDTKNRLFQEFLKPGDVIVFPKGLLHFCLNSGYETATIFSVYNSQNPGVVNVN 184

Query: 186 GAMFEPADSSHFVDKFIERLKALSSLSDLKFNPNVTLPRYISL 229
           GAMFEPADSSHF+D F+++LKALSSL +LKFNPNVTLPRYISL
Sbjct: 185 GAMFEPADSSHFIDMFLDKLKALSSLPNLKFNPNVTLPRYISL 226

[Close]

BLAST of Lag0031209 vs. ExPASy TrEMBL
Match: A0A5D3DCK1 (Germin-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1199G00230 PE=3 SV=1)

HSP 1 Score: 360.1 bits (923), Expect = 6.6e-96
Identity = 177/223 (79.37%), Postives = 199/223 (89.24%), Query Frame = 0


Query: 6   SASLIFLLLFVLFSCLGISVADSDNFFDTCPAATNKSEFINGFPCKNPTIVTTSDFNSAK 65
           ++SLIFLLLFVLFSCLGIS+ADS+N FDTCP AT++ EFING+ CK+PT+VTTSDFNSAK
Sbjct: 7   ASSLIFLLLFVLFSCLGISLADSNNVFDTCPTATDRIEFINGYHCKSPTLVTTSDFNSAK 66

Query: 66  LTGPVNTDDRFGSLVKLVTAADFPGLNTLGLSISRTDLGTDAIVSPHSHPRATEMFFVNQ 125
           LT PVNTDDR   +VK+VTAADF GLNTLGLSISRTDL  DAIVSPHSHPRATEMFF+NQ
Sbjct: 67  LTEPVNTDDRINLVVKIVTAADFHGLNTLGLSISRTDLVIDAIVSPHSHPRATEMFFLNQ 126

Query: 126 GIVLAGFIDTKNRLFQNFLKAGDVIVFPKGLLHFCLNSGYETATIFSVYNSQNPGVVNVN 185
           GIVLAGFIDT+N+LFQ  LKAGDVIVFPKGLLHFCLNSGYE A  FSVYNSQNPGVVN+N
Sbjct: 127 GIVLAGFIDTRNQLFQTILKAGDVIVFPKGLLHFCLNSGYEPAISFSVYNSQNPGVVNIN 186

Query: 186 GAMFEPADSSHFVDKFIERLKALSSLSDLKFNPNVTLPRYISL 229
           GA+F+PADSSHF++KF++RL   SS S+ K NPNVTL RY SL
Sbjct: 187 GAVFDPADSSHFIEKFLKRLTVPSSQSNFKLNPNVTLSRYTSL 229

[Close]

BLAST of Lag0031209 vs. ExPASy TrEMBL
Match: A0A1S3CNX7 (Germin-like protein OS=Cucumis melo OX=3656 GN=LOC103503059 PE=3 SV=1)

HSP 1 Score: 360.1 bits (923), Expect = 6.6e-96
Identity = 177/223 (79.37%), Postives = 199/223 (89.24%), Query Frame = 0


Query: 6   SASLIFLLLFVLFSCLGISVADSDNFFDTCPAATNKSEFINGFPCKNPTIVTTSDFNSAK 65
           ++SLIFLLLFVLFSCLGIS+ADS+N FDTCP AT++ EFING+ CK+PT+VTTSDFNSAK
Sbjct: 7   ASSLIFLLLFVLFSCLGISLADSNNVFDTCPTATDRIEFINGYHCKSPTLVTTSDFNSAK 66

Query: 66  LTGPVNTDDRFGSLVKLVTAADFPGLNTLGLSISRTDLGTDAIVSPHSHPRATEMFFVNQ 125
           LT PVNTDDR   +VK+VTAADF GLNTLGLSISRTDL  DAIVSPHSHPRATEMFF+NQ
Sbjct: 67  LTEPVNTDDRINLVVKIVTAADFHGLNTLGLSISRTDLVIDAIVSPHSHPRATEMFFLNQ 126

Query: 126 GIVLAGFIDTKNRLFQNFLKAGDVIVFPKGLLHFCLNSGYETATIFSVYNSQNPGVVNVN 185
           GIVLAGFIDT+N+LFQ  LKAGDVIVFPKGLLHFCLNSGYE A  FSVYNSQNPGVVN+N
Sbjct: 127 GIVLAGFIDTRNQLFQTILKAGDVIVFPKGLLHFCLNSGYEPAISFSVYNSQNPGVVNIN 186

Query: 186 GAMFEPADSSHFVDKFIERLKALSSLSDLKFNPNVTLPRYISL 229
           GA+F+PADSSHF++KF++RL   SS S+ K NPNVTL RY SL
Sbjct: 187 GAVFDPADSSHFIEKFLKRLTVPSSQSNFKLNPNVTLSRYTSL 229

[Close]

BLAST of Lag0031209 vs. TAIR 10
Match: AT5G61750.1 (RmlC-like cupins superfamily protein )

HSP 1 Score: 209.9 bits (533), Expect = 2.1e-54
Identity = 103/172 (59.88%), Postives = 127/172 (73.84%), Query Frame = 0


Query: 16  VLFSCLGISVA-DSDNFFDTCPAATNKSE--FINGFPCKNPTIVTTSDFNSAKLTGPVNT 75
           ++F  + +SV+ DSDN  DTCP A  +    FING+PCKNPT +T  DF S KLT   +T
Sbjct: 7   IVFCAIFLSVSGDSDNMQDTCPTAPGEQSIFFINGYPCKNPTKITAQDFKSTKLTEAGDT 66

Query: 76  DDRFGSLVKLVTAADFPGLNTLGLSISRTDLGTDAIVSPHSHPRATEMFFVNQGIVLAGF 135
           D+   S V L+TA +FPGLNTLGLS+SRTDL  D  V  HSHPR++EM FV +G+V AGF
Sbjct: 67  DNYLQSNVTLLTALEFPGLNTLGLSVSRTDLERDGSVPFHSHPRSSEMLFVVKGVVFAGF 126

Query: 136 IDTKNRLFQNFLKAGDVIVFPKGLLHFCLNSGYETATIFSVYNSQNPGVVNV 185
           +DT N++FQ  L+ GDV VFPKGLLHFCL+ G+E AT FS YNSQNPGVVN+
Sbjct: 127 VDTNNKIFQTVLQKGDVFVFPKGLLHFCLSGGFEPATAFSFYNSQNPGVVNI 178

[Close]

BLAST of Lag0031209 vs. TAIR 10
Match: AT1G09560.1 (germin-like protein 5 )

HSP 1 Score: 147.1 bits (370), Expect = 1.7e-35
Identity = 80/186 (43.01%), Postives = 107/186 (57.53%), Query Frame = 0


Query: 4   LSSASLIFLLLFVLFSCLGISVADSDNFFDTCPAATNKSEFINGFPCKNPTIVTTSDFNS 63
           ++S +L  LLL    S    S AD D   D C A       INGFPCK+   VT++DF S
Sbjct: 1   MASPTLTLLLLLTTVSFFISSSADPDMLQDLCVADLPSGIKINGFPCKDAATVTSADFFS 60

Query: 64  AKLTGPVNTDDRFGSLVKLVTAADFPGLNTLGLSISRTDLGTDAIVSPHSHPRATEMFFV 123
             L  P  T++ FG+LV        PGLNTLG+S+SR D     +  PH+HPRATE+ FV
Sbjct: 61  QGLAKPGLTNNTFGALVTGANVMTIPGLNTLGVSLSRIDYAPGGLNPPHTHPRATEVVFV 120

Query: 124 NQGIVLAGFIDTKNRLFQNFLKAGDVIVFPKGLLHFCLNSGYETATIFSVYNSQNPGVVN 183
            +G +  GF+ T N+L    LK GDV  FPKGL+HF  N+G   A++ + +NSQ PG  +
Sbjct: 121 LEGTLDVGFLTTANKLISQSLKKGDVFAFPKGLVHFQKNNGDVPASVIAAFNSQLPGTQS 180

Query: 184 VNGAMF 190
           +   +F
Sbjct: 181 LGATLF 186

[Close]

BLAST of Lag0031209 vs. TAIR 10
Match: AT1G02335.1 (germin-like protein subfamily 2 member 2 precursor )

HSP 1 Score: 138.7 bits (348), Expect = 6.0e-33
Identity = 80/208 (38.46%), Postives = 109/208 (52.40%), Query Frame = 0


Query: 14  LFVLFSCL---GISVADSDNFFDTCPAATNKSEFINGFPCKNPTIVTTSDFNSAKLTGPV 73
           + +  SC+    I   D D   D C A  +    +NGFPCK    +T SDF  A ++ P 
Sbjct: 8   IIIALSCIMITSIRAYDPDALQDLCVADKSHGTKLNGFPCKETLNITESDFFFAGISKPA 67

Query: 74  NTDDRFGSLVKLVTAADFPGLNTLGLSISRTDLGTDAIVSPHSHPRATEMFFVNQGIVLA 133
             +   GS V        PGLNTL +S++R D     +  PH+HPRATE+ +V +G +  
Sbjct: 68  VINSTMGSAVTGANVEKIPGLNTLSVSLARIDYAPGGLNPPHTHPRATEVVYVLEGELEV 127

Query: 134 GFIDTKNRLFQNFLKAGDVIVFPKGLLHFCLNSGYETATIFSVYNSQNPGVVNVNGAMF- 193
           GFI T N+LF   +K G+V VFP+GL+HF  N+G   A++ S +NSQ PG  +V   +F 
Sbjct: 128 GFITTANKLFTKTIKIGEVFVFPRGLVHFQKNNGKSPASVLSAFNSQLPGTASVAATLFA 187

Query: 194 -EPA-----------DSSHFVDKFIERL 206
            EPA             S  VDK  ERL
Sbjct: 188 AEPALPEDVLTKTFQVGSKMVDKIKERL 215

[Close]

BLAST of Lag0031209 vs. TAIR 10
Match: AT1G18970.1 (germin-like protein 4 )

HSP 1 Score: 135.6 bits (340), Expect = 5.1e-32
Identity = 76/179 (42.46%), Postives = 101/179 (56.42%), Query Frame = 0


Query: 11  FLLLFVLFSCLGISVADSDNFFDTCPAATNKSEFINGFPCKNPTIVTTSDFNSAKLTGPV 70
           FLL+F LF    +S +DSD   D C      S  INGFPCK  + V+ SDF  + L GP+
Sbjct: 14  FLLMFCLFVIPSLS-SDSDPLQDFCVGDLKASASINGFPCK--SAVSASDFFYSGLGGPL 73

Query: 71  NTDDRFGSLVKLVTAADFPGLNTLGLSISRTDLGTDAIVSPHSHPRATEMFFVNQGIVLA 130
           +T +  G  V       FPGLNTLG+S++  +L    +  PH HPRATE+  V +G V  
Sbjct: 74  DTSNPNGVTVAPANVLTFPGLNTLGISMNNVELAPGGVNPPHLHPRATEVGTVIEGSVFV 133

Query: 131 GFIDTKNRLFQNFLKAGDVIVFPKGLLHFCLNSGYETATIFSVYNSQNPGVVNVNGAMF 190
           GF+ T N LF   L AG+  V P+GL+HF  N G   A + + +NSQ PG V +   +F
Sbjct: 134 GFLSTNNTLFSKVLNAGEAFVIPRGLVHFQWNVGQVKARMITAFNSQLPGAVVLPSTLF 189

[Close]

BLAST of Lag0031209 vs. TAIR 10
Match: AT1G18980.1 (RmlC-like cupins superfamily protein )

HSP 1 Score: 130.6 bits (327), Expect = 1.6e-30
Identity = 77/185 (41.62%), Postives = 100/185 (54.05%), Query Frame = 0


Query: 5   SSASLIFLLLFVLFSCLGISVADSDNFFDTCPAATNKSEFINGFPCKNPTIVTTSDFNSA 64
           SS    FLL+  +F    +S +DSD   D C      S  INGFPCK+   V+ SDF  +
Sbjct: 8   SSLFRSFLLVICVFVIPSLS-SDSDPLQDFCVGDLKASPSINGFPCKSS--VSASDFFFS 67

Query: 65  KLTGPVNTDDRFGSLVKLVTAADFPGLNTLGLSISRTDLGTDAIVSPHSHPRATEMFFVN 124
            L GP+NT    G  V       FPGLNTLGLS++  +     +  PHSHPRATE   V 
Sbjct: 68  GLGGPLNTSTPNGVAVSPANVLTFPGLNTLGLSMNNVEFAPGGVNPPHSHPRATEAGVVI 127

Query: 125 QGIVLAGFIDTKNRLFQNFLKAGDVIVFPKGLLHFCLNSGYETATIFSVYNSQNPGVVNV 184
           +G V  GF+ T N LF   L AG++ V P+GL+HF  N G   A + + +NSQ PG   +
Sbjct: 128 EGSVFVGFLTTNNTLFSKVLNAGEMFVVPRGLVHFQWNVGKVKARLITSFNSQLPGSAVL 187

Query: 185 NGAMF 190
              +F
Sbjct: 188 PSTLF 189

The following BLAST results are available for this feature:

BLAST of Lag0031209 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023522282.1	3.5e-107	88.34	germin-like protein subfamily 3 member 4 [Cucurbita pepo subsp. pepo]	[more]
XP_022153439.1	3.5e-107	86.84	germin-like protein subfamily 3 member 4 [Momordica charantia]	[more]
KAG6606852.1	5.9e-107	88.74	Germin-like protein subfamily 3 member 4, partial [Cucurbita argyrosperma subsp....	[more]
XP_022948659.1	2.2e-106	87.89	germin-like protein subfamily 3 member 4 [Cucurbita moschata] >XP_022948660.1 ge...	[more]
XP_022998490.1	6.5e-106	87.00	germin-like protein subfamily 3 member 4 [Cucurbita maxima]	[more]

BLAST of Lag0031209 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FLT3	3.0e-53	59.88	Germin-like protein subfamily 3 member 4 OS=Arabidopsis thaliana OX=3702 GN=At5g...	[more]
Q2R352	2.5e-44	49.74	Germin-like protein 11-1 OS=Oryza sativa subsp. japonica OX=39947 GN=Os11g053735...	[more]
P94014	2.4e-34	43.01	Germin-like protein subfamily 2 member 1 OS=Arabidopsis thaliana OX=3702 GN=GLP4...	[more]
Q9S8P4	5.9e-33	43.82	Rhicadhesin receptor OS=Pisum sativum OX=3888 GN=GER1 PE=1 SV=2	[more]
P45852	3.8e-32	44.51	Germin-like protein OS=Mesembryanthemum crystallinum OX=3544 PE=2 SV=1	[more]

BLAST of Lag0031209 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DJ19	1.7e-107	86.84	Germin-like protein OS=Momordica charantia OX=3673 GN=LOC111020943 PE=3 SV=1	[more]
A0A6J1GAI2	1.1e-106	87.89	Germin-like protein OS=Cucurbita moschata OX=3662 GN=LOC111452269 PE=3 SV=1	[more]
A0A6J1KCN7	3.2e-106	87.00	Germin-like protein OS=Cucurbita maxima OX=3661 GN=LOC111493105 PE=3 SV=1	[more]
A0A5D3DCK1	6.6e-96	79.37	Germin-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1199...	[more]
A0A1S3CNX7	6.6e-96	79.37	Germin-like protein OS=Cucumis melo OX=3656 GN=LOC103503059 PE=3 SV=1	[more]

BLAST of Lag0031209 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G61750.1	2.1e-54	59.88	RmlC-like cupins superfamily protein	[more]
AT1G09560.1	1.7e-35	43.01	germin-like protein 5	[more]
AT1G02335.1	6.0e-33	38.46	germin-like protein subfamily 2 member 2 precursor	[more]
AT1G18970.1	5.1e-32	42.46	germin-like protein 4	[more]
AT1G18980.1	1.6e-30	41.62	RmlC-like cupins superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001929	Germin	PRINTS	PR00325	GERMIN	coord: 112..132 score: 53.38 coord: 175..190 score: 41.42 coord: 142..162 score: 52.05
IPR006045	Cupin 1	SMART	SM00835	Cupin_1_3	coord: 64..190 e-value: 2.9E-20 score: 83.3
IPR006045	Cupin 1	PFAM	PF00190	Cupin_1	coord: 69..186 e-value: 6.1E-30 score: 103.8
IPR014710	RmlC-like jelly roll fold	GENE3D	2.60.120.10	Jelly Rolls	coord: 26..210 e-value: 9.4E-56 score: 190.4
None	No IPR available	PANTHER	PTHR31238	GERMIN-LIKE PROTEIN SUBFAMILY 3 MEMBER 3	coord: 14..208
None	No IPR available	PANTHER	PTHR31238:SF3	GERMIN-LIKE PROTEIN SUBFAMILY 3 MEMBER 4	coord: 14..208
None	No IPR available	PROSITE	PS51257	PROKAR_LIPOPROTEIN	coord: 1..20 score: 6.0
None	No IPR available	CDD	cd02241	cupin_OxOx	coord: 27..200 e-value: 8.7584E-66 score: 199.744
IPR011051	RmlC-like cupin domain superfamily	SUPERFAMILY	51182	RmlC-like cupins	coord: 35..186

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0031209.1	Lag0031209.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0048046	apoplast
molecular_function	GO:0030145	manganese ion binding