Lag0031186 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0031186
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionO-fucosyltransferase family protein
Locationchr11: 5616246 .. 5619077 (-)
RNA-Seq ExpressionLag0031186
SyntenyLag0031186
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGAAGAAGAACAGTAATTCCAAGAAGCAATGTTACATTTCTGTTCCATCTCAAATCATTAACTCTCTGTCTTCGTCTTCTCTTCAATCCCTTCTCGATTCGCCGAAGAAATCTTCTAAAACTTACAACAAGTATCTCAGTCTTTCCACTTTCAGAAGCTCCAAGAAGCTATGGCTTCTCGCTATTTTCCTCTTTGGCCTTTTGGGCATGTTGAAGCTGAGTTTTAATCTCGGCCCTTTAATCGCTTATGTTTCGTACCCGTGTTTCACGTCTCAATCGCAGTCGCAGGGTTTTGTCTCTAATGGGTTTTTGAAATCGGGTGTAATCGATACAGAAAAGGAGAATGTTCTTCAGGATGAGATTGTGAACGATTCTGTTCAGTCGCTTCGGCCGATTTCAAATGGGGTTTTGAAATCGGATGAGGGTTTTGGAGAGGAAAATGAACAGCAGGAGTTCTGGAAGCAACCTGATGGATTGGGGTACAAACCGTGTTTAGATTTCAGCCAAGAATATAAGAAATCAACCACTGGAATCGTTAGTGAAAGGACTAAGTATCTGATGGTTGTTGTTTCTGGTGGGATGAATCAACAGAGAAATCAGATCGTTGATGCTGTTGTGATTGCTAGAATTCTTGGAGCTGCTCTGGTTGTTCCAATCTTGCAAGTGAACGTAATCTGGGGAGATGAAAGGTAACAAAACACGATTGACATATTGATTTTAAGTAGAAATGCATTTTTCTCTCTTTGATTGATTAAGTTTCTGATTCATAAGAACTGAAACATATTCTCCGTTTTATTGTGTATCAGTTGCTAAGTTTGTTTTTCTGTGTTCTGTTTGATGGTTGCAGTGAATTTTCTGATATATTTGATTTGGAGCATTTTAAGAGTGTTCTGGCCGATGATGTTCATGTAGTTTCATCTCTGCCATCGACACATCTGATGACTCGTCCGGTGGAGGAAAAATCGCCTCCGCATCAGGTCTCGCCGAGTTGGATTCGGTCGCGGTACCTCAGGAAGGTGAGGATTGTCAAAGGAATTTTGGTGTTTGTTCTTCTAGCTGCCATGGCTTCTTTTTGAAGCTCACTGCTAAAAAAGTGGCAAGCTTCTTGATTGTTAGGAAAGGAATCTGTTGGTTACTGCTCTGCTAGTTTTCATTATATCCTCACCATATAATTTACTCTTGCTCATCACAAAGAATCTTTGAAAATTGCTTTTGAATGTGTCACACAACATATTTGAACTAAATTATAACACATTTTGATGTTTGTAGAAGAGCTTTTGAGACTGCCCACGTCGCGATAATGCATGGATATCGGATTTGAAGTTTATATGCTAACTACTAAACTCGAAAGTTTAAATTGATAGGTTAAAGTAAATTTACTCTTTTTACATGTGGGCTCAGATGTTAATTTGATTGATGAAGCCTAACACGTGAACTATTAATATGAAGTGGAGTAAATATCATGTTAACCACTAAACTAAAATCCTTAAGCTTTTAGGTTAATGGTGATTTAACGATAAATAATTACTCGTGCAGTTGGTTTCATAGATTCTGTTAACTATAAATGTATGCCCTGTCTGTTTAGGTCATACATACATACATACCCTGTCTGTTAAGGTCATACATATATACCCTGTCTAAGGTTCACACATATATGCCCTGTCTGTTAAGGTTGACCAAACAGCTACTAAAAGTTTTTGTGGCATTTTTCTGTCCTTGTTATGTTTTTTACCAGACTTCATGTTCTTTATCCCTGCAAAATGTTTCTGTTAGTAGCCTGACGATACCAACATGTGTGTATTGGATTTTGCAGCTTAGAAGAGAAGGGGTACTGCTACTGCGCGGCTTGGACTCGAGGCTTTCCAAGGATCTTCCTTCTGATCTCCAAAAGCTTAGATGCAAGGTATTATAGGTTTTTTCATCTTGTTATAACCTCTTTGCTCTCTTCATCAGTTACTTGTCGCTTAGACTTGATCGCGGTGTATGCAGTATATAATTCAATTACTGCTTTCAATACTTATATGATACATCAATTTGAGTAGTACATCTTGATTGAAACATATGAGTTCTTTTGCTGCTGCTTTATAAGTAATCCTTTTGCCATAGTTAGTTCTTAAGAAATGCTGGGAATATCAGTGTAAATTGTTGTTCTTTTTCCTCTTTGGCCTTCAGGTTGCTTTTCATGCCTTGAGGTTTGCTCCACCAATTGTGGAACTAGGTAACAAGCTCACCGAAAGAATGCGAAGCAAAGGACCGTACCTTGCTCTTCATTTACGAATGGAGAAGGACGTATGGGTGAGAACTGGTTGTCTACCTGGGTTGAGCCCCGAATATGATGAGATGATAAACAATGAAAGAATACGACGACCCGAACTCTTAACTGCTAGATCAAACATGAGCTACCATGACAGAAAACTTGCAGGCCTTTGCCCCCTGAATGCCTATGAAGTTATGAGGTAAAGATTTCTTAACCATATGCTTGTCATTGTTAATCATTTCCAATTACGGTTCTGGTCGACTAAATTTCGCAACTTATTCAGGTTACTGAAAGCTCTCGGAGCTCCAAGGGATACAAGAATATACTGGGCCGGAGGAGAACCATTGGGAGGAAAAGAAGCCCTGCAACCATTAACAAGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGAGTTCCCGCATTTCTACAACAAGGAAGATCTCGCTTTGCCGAGAGAACTCGAACCTTTTGCCAAGAAAGCTTCCTTGATGGCTGCTATTGA

mRNA sequence

ATGGCGAAGAAGAACAGTAATTCCAAGAAGCAATGTTACATTTCTGTTCCATCTCAAATCATTAACTCTCTGTCTTCGTCTTCTCTTCAATCCCTTCTCGATTCGCCGAAGAAATCTTCTAAAACTTACAACAAGTATCTCAGTCTTTCCACTTTCAGAAGCTCCAAGAAGCTATGGCTTCTCGCTATTTTCCTCTTTGGCCTTTTGGGCATGTTGAAGCTGAGTTTTAATCTCGGCCCTTTAATCGCTTATGTTTCGTACCCGTGTTTCACGTCTCAATCGCAGTCGCAGGGTTTTGTCTCTAATGGGTTTTTGAAATCGGGTGTAATCGATACAGAAAAGGAGAATGTTCTTCAGGATGAGATTGTGAACGATTCTGTTCAGTCGCTTCGGCCGATTTCAAATGGGGTTTTGAAATCGGATGAGGGTTTTGGAGAGGAAAATGAACAGCAGGAGTTCTGGAAGCAACCTGATGGATTGGGGTACAAACCGTGTTTAGATTTCAGCCAAGAATATAAGAAATCAACCACTGGAATCGTTAGTGAAAGGACTAAGTATCTGATGGTTGTTGTTTCTGGTGGGATGAATCAACAGAGAAATCAGATCGTTGATGCTGTTGTGATTGCTAGAATTCTTGGAGCTGCTCTGGTTGTTCCAATCTTGCAAGTGAACGTAATCTGGGGAGATGAAAGTGAATTTTCTGATATATTTGATTTGGAGCATTTTAAGAGTGTTCTGGCCGATGATGTTCATGTAGTTTCATCTCTGCCATCGACACATCTGATGACTCGTCCGGTGGAGGAAAAATCGCCTCCGCATCAGGTCTCGCCGAGTTGGATTCGGTCGCGGTACCTCAGGAAGCTTAGAAGAGAAGGGGTACTGCTACTGCGCGGCTTGGACTCGAGGCTTTCCAAGGATCTTCCTTCTGATCTCCAAAAGCTTAGATGCAAGGTTGCTTTTCATGCCTTGAGGTTTGCTCCACCAATTGTGGAACTAGGTAACAAGCTCACCGAAAGAATGCGAAGCAAAGGACCGTACCTTGCTCTTCATTTACGAATGGAGAAGGACGTATGGGTGAGAACTGGTTGTCTACCTGGGTTGAGCCCCGAATATGATGAGATGATAAACAATGAAAGAATACGACGACCCGAACTCTTAACTGCTAGATCAAACATGAGCTACCATGACAGAAAACTTGCAGGCCTTTGCCCCCTGAATGCCTATGAAGTTATGAGGTTACTGAAAGCTCTCGGAGCTCCAAGGGATACAAGAATATACTGGGCCGGAGGAGAACCATTGGGAGGAAAAGAAGCCCTGCAACCATTAACAAGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGAGTTCCCGCATTTCTACAACAAGGAAGATCTCGCTTTGCCGAGAGAACTCGAACCTTTTGCCAAGAAAGCTTCCTTGATGGCTGCTATTGA

Coding sequence (CDS)

ATGGCGAAGAAGAACAGTAATTCCAAGAAGCAATGTTACATTTCTGTTCCATCTCAAATCATTAACTCTCTGTCTTCGTCTTCTCTTCAATCCCTTCTCGATTCGCCGAAGAAATCTTCTAAAACTTACAACAAGTATCTCAGTCTTTCCACTTTCAGAAGCTCCAAGAAGCTATGGCTTCTCGCTATTTTCCTCTTTGGCCTTTTGGGCATGTTGAAGCTGAGTTTTAATCTCGGCCCTTTAATCGCTTATGTTTCGTACCCGTGTTTCACGTCTCAATCGCAGTCGCAGGGTTTTGTCTCTAATGGGTTTTTGAAATCGGGTGTAATCGATACAGAAAAGGAGAATGTTCTTCAGGATGAGATTGTGAACGATTCTGTTCAGTCGCTTCGGCCGATTTCAAATGGGGTTTTGAAATCGGATGAGGGTTTTGGAGAGGAAAATGAACAGCAGGAGTTCTGGAAGCAACCTGATGGATTGGGGTACAAACCGTGTTTAGATTTCAGCCAAGAATATAAGAAATCAACCACTGGAATCGTTAGTGAAAGGACTAAGTATCTGATGGTTGTTGTTTCTGGTGGGATGAATCAACAGAGAAATCAGATCGTTGATGCTGTTGTGATTGCTAGAATTCTTGGAGCTGCTCTGGTTGTTCCAATCTTGCAAGTGAACGTAATCTGGGGAGATGAAAGTGAATTTTCTGATATATTTGATTTGGAGCATTTTAAGAGTGTTCTGGCCGATGATGTTCATGTAGTTTCATCTCTGCCATCGACACATCTGATGACTCGTCCGGTGGAGGAAAAATCGCCTCCGCATCAGGTCTCGCCGAGTTGGATTCGGTCGCGGTACCTCAGGAAGCTTAGAAGAGAAGGGGTACTGCTACTGCGCGGCTTGGACTCGAGGCTTTCCAAGGATCTTCCTTCTGATCTCCAAAAGCTTAGATGCAAGGTTGCTTTTCATGCCTTGAGGTTTGCTCCACCAATTGTGGAACTAGGTAACAAGCTCACCGAAAGAATGCGAAGCAAAGGACCGTACCTTGCTCTTCATTTACGAATGGAGAAGGACGTATGGGTGAGAACTGGTTGTCTACCTGGGTTGAGCCCCGAATATGATGAGATGATAAACAATGAAAGAATACGACGACCCGAACTCTTAACTGCTAGATCAAACATGAGCTACCATGACAGAAAACTTGCAGGCCTTTGCCCCCTGAATGCCTATGAAGTTATGAGGTTACTGAAAGCTCTCGGAGCTCCAAGGGATACAAGAATATACTGGGCCGGAGGAGAACCATTGGGAGGAAAAGAAGCCCTGCAACCATTAACAAGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGAGTTCCCGCATTTCTACAACAAGGAAGATCTCGCTTTGCCGAGAGAACTCGAACCTTTTGCCAAGAAAGCTTCCTTGATGGCTGCTATTGA

Protein sequence

MAKKNSNSKKQCYISVPSQIINSLSSSSLQSLLDSPKKSSKTYNKYLSLSTFRSSKKLWLLAIFLFGLLGMLKLSFNLGPLIAYVSYPCFTSQSQSQGFVSNGFLKSGVIDTEKENVLQDEIVNDSVQSLRPISNGVLKSDEGFGEENEQQEFWKQPDGLGYKPCLDFSQEYKKSTTGIVSERTKYLMVVVSGGMNQQRNQIVDAVVIARILGAALVVPILQVNVIWGDESEFSDIFDLEHFKSVLADDVHVVSSLPSTHLMTRPVEEKSPPHQVSPSWIRSRYLRKLRREGVLLLRGLDSRLSKDLPSDLQKLRCKVAFHALRFAPPIVELGNKLTERMRSKGPYLALHLRMEKDVWVRTGCLPGLSPEYDEMINNERIRRPELLTARSNMSYHDRKLAGLCPLNAYEVMRLLKALGAPRDTRIYWAGGEPLGGKEALQPLTRXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVPAFLQQGRSRFAERTRTFCQESFLDGCY
Homology
BLAST of Lag0031186 vs. NCBI nr
Match: XP_022935474.1 (O-fucosyltransferase 20-like [Cucurbita moschata] >KAG7018100.1 hypothetical protein SDJN02_19967 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 818.1 bits (2112), Expect = 4.1e-233
Identity = 413/444 (93.02%), Postives = 428/444 (96.40%), Query Frame = 0

Query: 1   MAKKNSNSKKQCYISVPSQIINSLSSSSLQSLLDSPKKSSKTYNKYLSLSTFRSSKKLWL 60
           MAKKNSNSKKQCYISVPSQIINSLSSSSLQSLLDSPKKSSK Y+KYLSL T+R+SKKLWL
Sbjct: 1   MAKKNSNSKKQCYISVPSQIINSLSSSSLQSLLDSPKKSSKHYDKYLSLFTYRASKKLWL 60

Query: 61  LAIFLFGLLGMLKLSFNLGPLIAYVSYPCFTSQSQSQGFVSNGFLKSGVIDTEKENVLQD 120
           LAIFLFG LGMLKL+FNL PLIAYVSYPCFTSQSQSQG VSNG LKSGVID E+EN L+D
Sbjct: 61  LAIFLFGFLGMLKLTFNLDPLIAYVSYPCFTSQSQSQGLVSNGVLKSGVIDRERENALKD 120

Query: 121 EIVNDSVQSLRPISNGVLKSDEGFGEENEQQEFWKQPDGLGYKPCLDFSQEYKKSTTGIV 180
           E +NDS+QSLRPISNGVLKSDEGFG+ NE QEFWKQPDGLGYKPCLDFSQEYKK+TTG++
Sbjct: 121 ESMNDSIQSLRPISNGVLKSDEGFGDGNEPQEFWKQPDGLGYKPCLDFSQEYKKTTTGVI 180

Query: 181 SERTKYLMVVVSGGMNQQRNQIVDAVVIARILGAALVVPILQVNVIWGDESEFSDIFDLE 240
           SERTKYLMVVVSGGMNQQRNQIVDAVVIARILGAALVVPI QVNVIWGDESEFSDIFDLE
Sbjct: 181 SERTKYLMVVVSGGMNQQRNQIVDAVVIARILGAALVVPIFQVNVIWGDESEFSDIFDLE 240

Query: 241 HFKSVLADDVHVVSSLPSTHLMTRPVEEKSPPHQVSPSWIRSRYLRKLRREGVLLLRGLD 300
           HFK+VLADDVH+VSSLPSTHLMTRPVEEKS PH VSPSWIRSRYLRKLRREGVLLLRGLD
Sbjct: 241 HFKNVLADDVHIVSSLPSTHLMTRPVEEKSTPHHVSPSWIRSRYLRKLRREGVLLLRGLD 300

Query: 301 SRLSKDLPSDLQKLRCKVAFHALRFAPPIVELGNKLTERMRSKGPYLALHLRMEKDVWVR 360
           SRLSKDLPSDLQKLRCKVAFHALRFAPPIVELGNKLTERMRSKGPYLALHLRMEKDVWVR
Sbjct: 301 SRLSKDLPSDLQKLRCKVAFHALRFAPPIVELGNKLTERMRSKGPYLALHLRMEKDVWVR 360

Query: 361 TGCLPGLSPEYDEMINNERIRRPELLTARSNMSYHDRKLAGLCPLNAYEVMRLLKALGAP 420
           TGCLPGLSPEYDEMINNERIRRPELLTARSNMSYHDRKLAGLCPLNAYEVMRLLKALGAP
Sbjct: 361 TGCLPGLSPEYDEMINNERIRRPELLTARSNMSYHDRKLAGLCPLNAYEVMRLLKALGAP 420

Query: 421 RDTRIYWAGGEPLGGKEALQPLTR 445
            DTRIYWAGG+PLGGKEALQPL R
Sbjct: 421 GDTRIYWAGGQPLGGKEALQPLIR 444

BLAST of Lag0031186 vs. NCBI nr
Match: KAG6581598.1 (O-fucosyltransferase 20, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 818.1 bits (2112), Expect = 4.1e-233
Identity = 413/444 (93.02%), Postives = 428/444 (96.40%), Query Frame = 0

Query: 1   MAKKNSNSKKQCYISVPSQIINSLSSSSLQSLLDSPKKSSKTYNKYLSLSTFRSSKKLWL 60
           MAKKNSNSKKQCYISVPSQIINSLSSSSLQSLLDSPKKSSK Y+KYLSL T+R+SKKLWL
Sbjct: 1   MAKKNSNSKKQCYISVPSQIINSLSSSSLQSLLDSPKKSSKHYDKYLSLFTYRASKKLWL 60

Query: 61  LAIFLFGLLGMLKLSFNLGPLIAYVSYPCFTSQSQSQGFVSNGFLKSGVIDTEKENVLQD 120
           LAIFLFG LGMLKL+FNL PLIAYVSYPCFTSQSQSQG VSNG LKSGVID E+EN L+D
Sbjct: 61  LAIFLFGFLGMLKLTFNLDPLIAYVSYPCFTSQSQSQGLVSNGVLKSGVIDRERENALKD 120

Query: 121 EIVNDSVQSLRPISNGVLKSDEGFGEENEQQEFWKQPDGLGYKPCLDFSQEYKKSTTGIV 180
           E +NDS+QSLRPISNGVLKSDEGFG+ NE QEFWKQPDGLGYKPCLDFSQEYKK+TTG++
Sbjct: 121 ESMNDSIQSLRPISNGVLKSDEGFGDGNEPQEFWKQPDGLGYKPCLDFSQEYKKTTTGVI 180

Query: 181 SERTKYLMVVVSGGMNQQRNQIVDAVVIARILGAALVVPILQVNVIWGDESEFSDIFDLE 240
           SERTKYLMVVVSGGMNQQRNQIVDAVVIARILGAALVVPI QVNVIWGDESEFSDIFDLE
Sbjct: 181 SERTKYLMVVVSGGMNQQRNQIVDAVVIARILGAALVVPIFQVNVIWGDESEFSDIFDLE 240

Query: 241 HFKSVLADDVHVVSSLPSTHLMTRPVEEKSPPHQVSPSWIRSRYLRKLRREGVLLLRGLD 300
           HFK+VLADDVH+VSSLPSTHLMTRPVEEKS PH VSPSWIRSRYLRKLRREGVLLLRGLD
Sbjct: 241 HFKNVLADDVHIVSSLPSTHLMTRPVEEKSTPHHVSPSWIRSRYLRKLRREGVLLLRGLD 300

Query: 301 SRLSKDLPSDLQKLRCKVAFHALRFAPPIVELGNKLTERMRSKGPYLALHLRMEKDVWVR 360
           SRLSKDLPSDLQKLRCKVAFHALRFAPPIVELGNKLTERMRSKGPYLALHLRMEKDVWVR
Sbjct: 301 SRLSKDLPSDLQKLRCKVAFHALRFAPPIVELGNKLTERMRSKGPYLALHLRMEKDVWVR 360

Query: 361 TGCLPGLSPEYDEMINNERIRRPELLTARSNMSYHDRKLAGLCPLNAYEVMRLLKALGAP 420
           TGCLPGLSPEYDEMINNERIRRPELLTARSNMSYHDRKLAGLCPLNAYEVMRLLKALGAP
Sbjct: 361 TGCLPGLSPEYDEMINNERIRRPELLTARSNMSYHDRKLAGLCPLNAYEVMRLLKALGAP 420

Query: 421 RDTRIYWAGGEPLGGKEALQPLTR 445
            DTRIYWAGG+PLGGKEALQPL R
Sbjct: 421 GDTRIYWAGGQPLGGKEALQPLIR 444

BLAST of Lag0031186 vs. NCBI nr
Match: XP_038906082.1 (O-fucosyltransferase 20 [Benincasa hispida])

HSP 1 Score: 816.2 bits (2107), Expect = 1.5e-232
Identity = 415/444 (93.47%), Postives = 428/444 (96.40%), Query Frame = 0

Query: 1   MAKKNSNSKKQCYISVPSQIINSLSSSSLQSLLDSPKKSSKTYNKYLSLSTFRSSKKLWL 60
           MAKKNSNSKKQ YISVPSQIINSLSSSSLQSLLDSPKKSSK YNK+LSLST+RSSKKLWL
Sbjct: 1   MAKKNSNSKKQSYISVPSQIINSLSSSSLQSLLDSPKKSSKNYNKFLSLSTYRSSKKLWL 60

Query: 61  LAIFLFGLLGMLKLSFNLGPLIAYVSYPCFTSQSQSQGFVSNGFLKSGVIDTEKENVLQD 120
           LAIFLFGL GMLKL+FNL PLI YVSYPCFTSQSQSQG VSNG LKSG+   E+EN L+D
Sbjct: 61  LAIFLFGLFGMLKLTFNLNPLIPYVSYPCFTSQSQSQGLVSNGVLKSGI---ERENALKD 120

Query: 121 EIVNDSVQSLRPISNGVLKSDEGFGEENEQQEFWKQPDGLGYKPCLDFSQEYKKSTTGIV 180
           E++NDS QSLRPISNGVLKSDEGFGE NEQQEFWKQPDGLGYKPCLDFS+EYKKST GIV
Sbjct: 121 EVMNDSFQSLRPISNGVLKSDEGFGEGNEQQEFWKQPDGLGYKPCLDFSEEYKKSTPGIV 180

Query: 181 SERTKYLMVVVSGGMNQQRNQIVDAVVIARILGAALVVPILQVNVIWGDESEFSDIFDLE 240
           SERTKYLMVVVSGGMNQQRNQIVDAVVIARILGAALVVPILQVNVIWGDESEFSDIFDLE
Sbjct: 181 SERTKYLMVVVSGGMNQQRNQIVDAVVIARILGAALVVPILQVNVIWGDESEFSDIFDLE 240

Query: 241 HFKSVLADDVHVVSSLPSTHLMTRPVEEKSPPHQVSPSWIRSRYLRKLRREGVLLLRGLD 300
           HFK+VLADDVH+VSSLPSTHLMTRPVEEKS PH VSP+WIRSRYLRKLRREGVLLLRGLD
Sbjct: 241 HFKNVLADDVHIVSSLPSTHLMTRPVEEKSTPHHVSPTWIRSRYLRKLRREGVLLLRGLD 300

Query: 301 SRLSKDLPSDLQKLRCKVAFHALRFAPPIVELGNKLTERMRSKGPYLALHLRMEKDVWVR 360
           SRLSKDLPSDLQKLRCKVAFHALRFAPPIVELGNKLTERMRSKGPYLALHLRMEKDVWVR
Sbjct: 301 SRLSKDLPSDLQKLRCKVAFHALRFAPPIVELGNKLTERMRSKGPYLALHLRMEKDVWVR 360

Query: 361 TGCLPGLSPEYDEMINNERIRRPELLTARSNMSYHDRKLAGLCPLNAYEVMRLLKALGAP 420
           TGCLPGLSPEYDEMINNERIRRPELLTARSNMSYHDRKLAGLCPLNAYEVMRLLKALGAP
Sbjct: 361 TGCLPGLSPEYDEMINNERIRRPELLTARSNMSYHDRKLAGLCPLNAYEVMRLLKALGAP 420

Query: 421 RDTRIYWAGGEPLGGKEALQPLTR 445
           RDTRIYWAGG+PLGGKEALQPLTR
Sbjct: 421 RDTRIYWAGGQPLGGKEALQPLTR 441

BLAST of Lag0031186 vs. NCBI nr
Match: XP_022983465.1 (O-fucosyltransferase 20-like [Cucurbita maxima])

HSP 1 Score: 815.1 bits (2104), Expect = 3.4e-232
Identity = 411/444 (92.57%), Postives = 427/444 (96.17%), Query Frame = 0

Query: 1   MAKKNSNSKKQCYISVPSQIINSLSSSSLQSLLDSPKKSSKTYNKYLSLSTFRSSKKLWL 60
           MAKKNSNSKKQCYISVPSQIINSLSSSSLQSLLDSPKKSSK Y+KYLSL T+R+SKKLWL
Sbjct: 1   MAKKNSNSKKQCYISVPSQIINSLSSSSLQSLLDSPKKSSKHYDKYLSLFTYRASKKLWL 60

Query: 61  LAIFLFGLLGMLKLSFNLGPLIAYVSYPCFTSQSQSQGFVSNGFLKSGVIDTEKENVLQD 120
           LAIFLFG LGMLKL+FNL PL AYVSYPCF SQSQSQG VSNG LKSGV+D E+EN L+D
Sbjct: 61  LAIFLFGFLGMLKLTFNLDPLFAYVSYPCFMSQSQSQGLVSNGALKSGVMDRERENALKD 120

Query: 121 EIVNDSVQSLRPISNGVLKSDEGFGEENEQQEFWKQPDGLGYKPCLDFSQEYKKSTTGIV 180
           E +NDS+QSLRPISNGVLKSDEGFG+ NEQQEFWKQPDGLGYKPCLDFSQEYKK+TTG++
Sbjct: 121 ESMNDSIQSLRPISNGVLKSDEGFGDGNEQQEFWKQPDGLGYKPCLDFSQEYKKTTTGVI 180

Query: 181 SERTKYLMVVVSGGMNQQRNQIVDAVVIARILGAALVVPILQVNVIWGDESEFSDIFDLE 240
           SERTKYLMVVVSGGMNQQRNQIVDAVVIARILGAALVVPI QVNVIWGDESEFSDIFDLE
Sbjct: 181 SERTKYLMVVVSGGMNQQRNQIVDAVVIARILGAALVVPIFQVNVIWGDESEFSDIFDLE 240

Query: 241 HFKSVLADDVHVVSSLPSTHLMTRPVEEKSPPHQVSPSWIRSRYLRKLRREGVLLLRGLD 300
           HFK+VLADDVH+VSSLPSTHLMTRPVEEKS PH VSPSWIRSRYLRKLRREGVLLLRGLD
Sbjct: 241 HFKNVLADDVHIVSSLPSTHLMTRPVEEKSTPHHVSPSWIRSRYLRKLRREGVLLLRGLD 300

Query: 301 SRLSKDLPSDLQKLRCKVAFHALRFAPPIVELGNKLTERMRSKGPYLALHLRMEKDVWVR 360
           SRLSKDLPSDLQKLRCKVAFHALRFAPPIVELGNKLTERMRSKGPYLALHLRMEKDVWVR
Sbjct: 301 SRLSKDLPSDLQKLRCKVAFHALRFAPPIVELGNKLTERMRSKGPYLALHLRMEKDVWVR 360

Query: 361 TGCLPGLSPEYDEMINNERIRRPELLTARSNMSYHDRKLAGLCPLNAYEVMRLLKALGAP 420
           TGCLPGLSPEYDEMINNERIRRPELLTARSNMSYHDRKLAGLCPLNAYEVMRLLKALGAP
Sbjct: 361 TGCLPGLSPEYDEMINNERIRRPELLTARSNMSYHDRKLAGLCPLNAYEVMRLLKALGAP 420

Query: 421 RDTRIYWAGGEPLGGKEALQPLTR 445
            DTRIYWAGG+PLGGKEALQPL R
Sbjct: 421 GDTRIYWAGGQPLGGKEALQPLIR 444

BLAST of Lag0031186 vs. NCBI nr
Match: XP_023528632.1 (O-fucosyltransferase 20-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 808.5 bits (2087), Expect = 3.2e-230
Identity = 408/444 (91.89%), Postives = 424/444 (95.50%), Query Frame = 0

Query: 1   MAKKNSNSKKQCYISVPSQIINSLSSSSLQSLLDSPKKSSKTYNKYLSLSTFRSSKKLWL 60
           MAKKNSNSKKQCYISVPSQIINSLSSSSLQSLLDSPKKSSK Y+KYLSL T+R+SKKLWL
Sbjct: 1   MAKKNSNSKKQCYISVPSQIINSLSSSSLQSLLDSPKKSSKHYDKYLSLFTYRASKKLWL 60

Query: 61  LAIFLFGLLGMLKLSFNLGPLIAYVSYPCFTSQSQSQGFVSNGFLKSGVIDTEKENVLQD 120
           LAIFLFG LGMLKL+ NL PLIAYVSYPCF SQSQSQG VSN  LKSG ID E+EN L+D
Sbjct: 61  LAIFLFGFLGMLKLTLNLDPLIAYVSYPCFMSQSQSQGLVSNRVLKSGAIDRERENALKD 120

Query: 121 EIVNDSVQSLRPISNGVLKSDEGFGEENEQQEFWKQPDGLGYKPCLDFSQEYKKSTTGIV 180
           E +NDS+QSLRPISNGVLKSDEGFG+ NEQQEFWKQPDGLGYKPCLDFSQEYK++TTG++
Sbjct: 121 ESMNDSIQSLRPISNGVLKSDEGFGDGNEQQEFWKQPDGLGYKPCLDFSQEYKRTTTGVI 180

Query: 181 SERTKYLMVVVSGGMNQQRNQIVDAVVIARILGAALVVPILQVNVIWGDESEFSDIFDLE 240
           SERTKYLMVVVSGGMNQQRNQIVDAVVIARILGAALVVPI QVNVIWGDESEFSDIFDLE
Sbjct: 181 SERTKYLMVVVSGGMNQQRNQIVDAVVIARILGAALVVPIFQVNVIWGDESEFSDIFDLE 240

Query: 241 HFKSVLADDVHVVSSLPSTHLMTRPVEEKSPPHQVSPSWIRSRYLRKLRREGVLLLRGLD 300
           HFK+VLADDVH+VSSLPSTHLMTRPVEEKS PH VSPSWIRSRYLRKLRREGVLLLRGLD
Sbjct: 241 HFKNVLADDVHIVSSLPSTHLMTRPVEEKSTPHHVSPSWIRSRYLRKLRREGVLLLRGLD 300

Query: 301 SRLSKDLPSDLQKLRCKVAFHALRFAPPIVELGNKLTERMRSKGPYLALHLRMEKDVWVR 360
           SRLSKDLPSDLQKLRCKVAFHALRFAPPIVE GNKLTERMRSKGPYLALHLRMEKDVWVR
Sbjct: 301 SRLSKDLPSDLQKLRCKVAFHALRFAPPIVEFGNKLTERMRSKGPYLALHLRMEKDVWVR 360

Query: 361 TGCLPGLSPEYDEMINNERIRRPELLTARSNMSYHDRKLAGLCPLNAYEVMRLLKALGAP 420
           TGCLPGLSPEYDEMINNERIRRPELLTARSNMSYHDRKLAGLCPLNAYEVMRLLKALGAP
Sbjct: 361 TGCLPGLSPEYDEMINNERIRRPELLTARSNMSYHDRKLAGLCPLNAYEVMRLLKALGAP 420

Query: 421 RDTRIYWAGGEPLGGKEALQPLTR 445
            DTRIYWAGG+PLGGKEALQPL R
Sbjct: 421 GDTRIYWAGGQPLGGKEALQPLIR 444

BLAST of Lag0031186 vs. ExPASy Swiss-Prot
Match: O64884 (O-fucosyltransferase 20 OS=Arabidopsis thaliana OX=3702 GN=OFUT20 PE=1 SV=1)

HSP 1 Score: 548.9 bits (1413), Expect = 6.0e-155
Identity = 292/454 (64.32%), Postives = 345/454 (75.99%), Query Frame = 0

Query: 4   KNSNS-----KKQCYISVPSQIINSLSSSSLQSLLDSPKKSSKTYNKYLSLSTFRSSKKL 63
           KNSNS     KK  YISVPSQIINSLSSSSLQSLL SPKKSS++ N++   S    + ++
Sbjct: 5   KNSNSNSFNKKKVSYISVPSQIINSLSSSSLQSLLVSPKKSSRSTNRF---SFSYRNPRI 64

Query: 64  WLLAIFLFGLLGMLKLSFNLGPL-IAYVSYPCFTSQSQ-----SQGFVSN-GFLKSGVID 123
           W   +FL  L GMLKL FN+ P+ + +  YPC T+Q        Q   S+ G  +  ++ 
Sbjct: 65  WFFTLFLVSLFGMLKLGFNVDPISLPFSRYPCSTTQQPLSFDGEQNAASHLGLAQEPILS 124

Query: 124 TEKENVLQDEIVNDSVQSLRPISNGVLKSDEGFGEE-NEQQEFWKQPDGLGYKPCLDFSQ 183
           T   N                 SN +++ + G  E    + +FWKQPDGLG+KPCL F+ 
Sbjct: 125 TGSSN-----------------SNAIIQLNGGKNETLLTEGDFWKQPDGLGFKPCLGFTS 184

Query: 184 EYKKSTTGIVSERTKYLMVVVSGGMNQQRNQIVDAVVIARILGAALVVPILQVNVIWGDE 243
           +Y+K +  I+  R KYL+VVVSGGMNQQRNQIVDAVVIARILGA+LVVP+LQVNVIWGDE
Sbjct: 185 QYRKDSNSILKNRWKYLLVVVSGGMNQQRNQIVDAVVIARILGASLVVPVLQVNVIWGDE 244

Query: 244 SEFSDIFDLEHFKSVLADDVHVVSSLPSTHLMTRPVEEKSPPHQVSPSWIRSRYLRKLRR 303
           SEF+DIFDLEHFK VLADDVH+VSSLPSTH+MTRPVEEK  P   SP WIR+ YL+++ R
Sbjct: 245 SEFADIFDLEHFKDVLADDVHIVSSLPSTHVMTRPVEEKRTPLHASPQWIRAHYLKRINR 304

Query: 304 EGVLLLRGLDSRLSKDLPSDLQKLRCKVAFHALRFAPPIVELGNKLTERMRSKGPYLALH 363
           E VLLLRGLDSRLSKDLPSDLQKLRCKVAF ALRF+P I+ELGNKL  RMR++G YL+LH
Sbjct: 305 ERVLLLRGLDSRLSKDLPSDLQKLRCKVAFQALRFSPRILELGNKLASRMRNQGQYLSLH 364

Query: 364 LRMEKDVWVRTGCLPGLSPEYDEMINNERIRRPELLTARSNMSYHDRKLAGLCPLNAYEV 423
           LRMEKDVWVRTGCLPGL+PEYDE++N+ER R PELLT RSNM+YH+RKLAGLCPL A EV
Sbjct: 365 LRMEKDVWVRTGCLPGLTPEYDEIVNSERERHPELLTGRSNMTYHERKLAGLCPLTALEV 424

Query: 424 MRLLKALGAPRDTRIYWAGGEPLGGKEALQPLTR 445
            RLLKAL AP+D RIYWAGGEPLGGKE L+PLT+
Sbjct: 425 TRLLKALEAPKDARIYWAGGEPLGGKEVLEPLTK 438

BLAST of Lag0031186 vs. ExPASy Swiss-Prot
Match: A0A0B5GR44 (O-fucosyltransferase 20 OS=Brassica napus OX=3708 GN=OFUT20 PE=2 SV=2)

HSP 1 Score: 527.3 bits (1357), Expect = 1.9e-148
Identity = 281/441 (63.72%), Postives = 335/441 (75.96%), Query Frame = 0

Query: 5   NSNSKKQCYISVPSQIINSLSSSSLQSLLDSPKKSSKTYNKYLSLSTFRSSKKLWLLAIF 64
           +S  KK  YISVPSQIINSLSSSSLQSLL SPKKSS+  N++    ++R + ++W L +F
Sbjct: 11  SSTKKKVSYISVPSQIINSLSSSSLQSLLVSPKKSSRCTNRF----SYR-NPRIWFLTLF 70

Query: 65  LFGLLGMLKLSFNLGPL-IAYVSYPCFTSQSQSQGFVSNGFLKSGVIDTEKENVLQDEIV 124
           L  L GMLKL  N+ P+ + +  YPC T        VS+               L     
Sbjct: 71  LVSLFGMLKLGLNVDPISLPFSRYPCSTGSFDEHHAVSH---------------LAFASK 130

Query: 125 NDSVQSLRPISNGVLKSDEGFGEENEQQEFWKQPDGLGYKPCLDFSQEYKKSTTGIVSER 184
           ND+  S    S+   + +E    E    +FWKQPDGLG+KPCL FS++Y+K +  I+  R
Sbjct: 131 NDTQSS----SSSEHRKNETLPTEG---DFWKQPDGLGFKPCLGFSRQYRKDSNSILKNR 190

Query: 185 TKYLMVVVSGGMNQQRNQIVDAVVIARILGAALVVPILQVNVIWGDESEFSDIFDLEHFK 244
            KYL+VVV+GGMNQQRNQIVDAV++ARILGA+LVVP+LQVNVIWGDESEF+DIFDLEHFK
Sbjct: 191 WKYLLVVVAGGMNQQRNQIVDAVIMARILGASLVVPVLQVNVIWGDESEFADIFDLEHFK 250

Query: 245 SVLADDVHVVSSLPSTHLMTRPVEEKSPPHQVSPSWIRSRYLRKLRREGVLLLRGLDSRL 304
           +VLADDVH+VSSLPSTH+MTRP EEK  P   SP WIR+ YL+++ RE VLLLRGLDSRL
Sbjct: 251 NVLADDVHIVSSLPSTHVMTRPAEEKRTPLHASPQWIRAHYLKRINRERVLLLRGLDSRL 310

Query: 305 SKDLPSDLQKLRCKVAFHALRFAPPIVELGNKLTERMRSKGPYLALHLRMEKDVWVRTGC 364
           S DLPSDLQKLRCKVA  ALRF+P I+ELGNKL  RM S+G YL+LHLRMEKDVWVRTG 
Sbjct: 311 SNDLPSDLQKLRCKVASQALRFSPRILELGNKLASRMLSEGQYLSLHLRMEKDVWVRTGS 370

Query: 365 LPGLSPEYDEMINNERIRRPELLTARSNMSYHDRKLAGLCPLNAYEVMRLLKALGAPRDT 424
           LPGL+PEYDE++N+ER R PELLT RSNM+Y++RKLAGLCPL A EV RLLKAL AP+D 
Sbjct: 371 LPGLTPEYDEIVNSERQRHPELLTGRSNMTYNERKLAGLCPLTALEVTRLLKALEAPKDA 424

Query: 425 RIYWAGGEPLGGKEALQPLTR 445
           RIYWAGGEPLGGKEAL+PLT+
Sbjct: 431 RIYWAGGEPLGGKEALEPLTK 424

BLAST of Lag0031186 vs. ExPASy Swiss-Prot
Match: Q9SFC4 (O-fucosyltransferase 24 OS=Arabidopsis thaliana OX=3702 GN=OFUT24 PE=2 SV=1)

HSP 1 Score: 498.0 bits (1281), Expect = 1.2e-139
Identity = 277/454 (61.01%), Postives = 334/454 (73.57%), Query Frame = 0

Query: 4   KNSNSKKQ-CYISVPSQIINSLSSSSLQSLLDSPKKSSKTYNKYLSLSTFRSSKKLWLLA 63
           KNSN K +  YISVP+QIINS+SSSSL   LD+      T   + +L     + KLW  +
Sbjct: 7   KNSNKKNRVSYISVPAQIINSVSSSSLHKFLDNKSSKKNTSKFFFNL----RNPKLWAFS 66

Query: 64  IFLFGLLGMLKLSFNLGPLIAYVSYPCFTSQSQSQGFVSNGFLKSGV-IDTEKENVLQDE 123
           +FL  +LG   +S  LG  +++       SQ QS    SNG  KS +     + +  Q E
Sbjct: 67  LFLLSILG---ISLRLGLCLSHFGSGDHESQLQSSD--SNGSPKSHMGFAYIRSSTTQVE 126

Query: 124 IVNDSVQSLRPISNGVLKSD------------EGFGEENEQQEFWKQPDGLGYKPCLDFS 183
           I N   +SL     GV K++             G G +++  +FWKQPDGLGYKPCLDFS
Sbjct: 127 ISNAKDRSL---DMGVEKNETFGGHQSHLIIPNGNGHDDKNYDFWKQPDGLGYKPCLDFS 186

Query: 184 QEYKKSTTGIVSERTKYLMVVVSGGMNQQRNQIVDAVVIARILGAALVVPILQVNVIWGD 243
            EY++ +  I+ ER KYLMVVVSGG+NQQ+ QIVDAVVIARILGA LVVPILQ+N+IWGD
Sbjct: 187 IEYRRESKKILVERRKYLMVVVSGGLNQQKIQIVDAVVIARILGAVLVVPILQINLIWGD 246

Query: 244 ESEFSDIFDLEHFKSVLADDVHVVSSLPSTHLMTRPVEEKSPPHQVSPSWIRSRYLRKLR 303
           ESEFSDIFDLE FKSVLA+DV +VS LP++ +MTRP E+ S P   SP WIRS Y ++  
Sbjct: 247 ESEFSDIFDLEQFKSVLANDVKIVSLLPASKVMTRPSEDGSMPFNASPQWIRSHYPKRFN 306

Query: 304 REGVLLLRGLDSRLSKDLPSDLQKLRCKVAFHALRFAPPIVELGNKLTERMRSKGPYLAL 363
           REGVLLLR LDSRLSKDLPSDLQKLRCKVAF AL+F+P ++E+G KL ERMRSKGPY+AL
Sbjct: 307 REGVLLLRRLDSRLSKDLPSDLQKLRCKVAFEALKFSPRVMEMGTKLAERMRSKGPYIAL 366

Query: 364 HLRMEKDVWVRTGCLPGLSPEYDEMINNERIRRPELLTARSNMSYHDRKLAGLCPLNAYE 423
           HLRMEKDVWVRTGCL GLS +YDE++N ERI+RPELLTA+S+M+ ++RKLAGLCPLNA E
Sbjct: 367 HLRMEKDVWVRTGCLSGLSSKYDEIVNIERIKRPELLTAKSSMTSNERKLAGLCPLNAKE 426

Query: 424 VMRLLKALGAPRDTRIYWAGGEPLGGKEALQPLT 444
           V RLL+ALGAPRD RIYWAGGEPLGGKEAL+PLT
Sbjct: 427 VTRLLRALGAPRDARIYWAGGEPLGGKEALKPLT 448

BLAST of Lag0031186 vs. ExPASy Swiss-Prot
Match: Q9FMW3 (O-fucosyltransferase 37 OS=Arabidopsis thaliana OX=3702 GN=OFUT37 PE=2 SV=1)

HSP 1 Score: 413.7 bits (1062), Expect = 3.0e-114
Identity = 207/363 (57.02%), Postives = 259/363 (71.35%), Query Frame = 0

Query: 82  IAYVSYPCFTSQSQSQGFVSNGFLKSGVIDTEKENVLQDEIVNDSVQSLRPISNGVLKSD 141
           I+++++   T  S      S   LK  + D +   +L    V+    +L P+     +  
Sbjct: 67  ISFLTFSLDTGSSTCVSTSSASSLKFFISDVDHRQILTSLAVS-GASTLFPLP---ARGG 126

Query: 142 EGFGEENEQQEFWKQPDGLGYKPCLDFSQEYKKSTTGIVSERTKYLMVVVSGGMNQQRNQ 201
                  E++EFWKQP+G GYKPCLDFS EYKK +  +  E+ ++L+VVVSGG+NQQRNQ
Sbjct: 127 HSGNMTEEEKEFWKQPNGEGYKPCLDFSLEYKKKSASVSKEKKRFLVVVVSGGLNQQRNQ 186

Query: 202 IVDAVVIARILGAALVVPILQVNVIWGDESEFSDIFDLEHFKSVLADDVHVVSSLPSTHL 261
           IVDAVVIA IL AALVVP+LQVN +WGDESEFSD+FD+EHFK  L  DV +VSSLPSTHL
Sbjct: 187 IVDAVVIAMILEAALVVPVLQVNRVWGDESEFSDLFDVEHFKKTLRSDVRIVSSLPSTHL 246

Query: 262 MTRPVEEKSPPHQVSPSWIRSRYLRKLRREGVLLLRGLDSRLSKDLPSDLQKLRCKVAFH 321
           M+R   E   P  VSP WIR++Y ++L  EG+L+L+GLDS+L+K+LP DLQKLRCKVAFH
Sbjct: 247 MSRQTIENQIPWDVSPVWIRAKYFKQLNEEGLLVLKGLDSKLAKNLPPDLQKLRCKVAFH 306

Query: 322 ALRFAPPIVELGNKLTERMRSKGPYLALHLRMEKDVWVRTGCLPGLSPEYDEMINNERIR 381
           ALRFA PI  LGNKLT RM  +GPY+ALHLR+EKDVWVRTGCL GL  E+D +I   R  
Sbjct: 307 ALRFAAPIENLGNKLTRRMWIEGPYIALHLRLEKDVWVRTGCLTGLGSEFDRIIAETRTS 366

Query: 382 RPELLTARSNMSYHDRKLAGLCPLNAYEVMRLLKALGAPRDTRIYWAGGEPLGGKEALQP 441
           +P  LT R N++Y +R+LAG CPLN YE+ RLLKALGAP +  IY AGGEP GG  AL+P
Sbjct: 367 QPRYLTGRLNLTYTERRLAGFCPLNVYEIARLLKALGAPSNASIYIAGGEPFGGSRALEP 425

Query: 442 LTR 445
           L +
Sbjct: 427 LAK 425

BLAST of Lag0031186 vs. ExPASy Swiss-Prot
Match: Q94BY4 (O-fucosyltransferase 35 OS=Arabidopsis thaliana OX=3702 GN=OFUT35 PE=2 SV=1)

HSP 1 Score: 191.8 bits (486), Expect = 1.9e-47
Identity = 117/303 (38.61%), Postives = 165/303 (54.46%), Query Frame = 0

Query: 137 VLKSDEGFGEENEQQEF---WKQPDGLGYKPCLDFSQEYKKSTTGIVSERTKYLMVVVSG 196
           V+ + +  G++N   EF   W +P+   +  C+D S+  KK    + +    YL++  +G
Sbjct: 206 VMVASDVVGDQNSVVEFSGVWAKPESGNFSRCIDSSRSRKK----LGANTNGYLLINANG 265

Query: 197 GMNQQRNQIVDAVVIARILGAALVVPILQVNVIWGDESEFSDIFDLEHFKSVLADDVHVV 256
           G+NQ R  I D V +A+I+ A LV+P L  +  W D+S F D+FD +HF   L DD+H+V
Sbjct: 266 GLNQMRFGICDMVAVAKIMKATLVLPSLDHSSYWADDSGFKDLFDWQHFIEELKDDIHIV 325

Query: 257 SSLPSTHLMTRPVEEKSPPHQVSPSWIRSRYLRKLRREGVLLLRGLDSRL-SKDLPSDLQ 316
             LPS      P   K+P       + +   L  L++  V+ L   DSRL + DLP  +Q
Sbjct: 326 EMLPSELAGIEPF-VKTPISWSKVGYYKKEVLPLLKQHIVMYLTHTDSRLANNDLPDSVQ 385

Query: 317 KLRCKVAFHALRFAPPIVELGNKLTERMR-SKGPYLALHLRMEKDVWVRTGCLPGLSPEY 376
           KLRC+V + AL+++ PI ELGN L  RMR  +GPYLALHLR EKD+   TGC   L+ E 
Sbjct: 386 KLRCRVNYRALKYSAPIEELGNVLVSRMRQDRGPYLALHLRYEKDMLAFTGCSHSLTAEE 445

Query: 377 DEMINNERIRRPELLTARSNMSYHDRKLAGLCPLNAYEVMRLLKALGAPRDTRIYWAGGE 435
           DE +   R           N +  +R+L G CPL   E   LL+AL  P  +RIY   GE
Sbjct: 446 DEELRQMRYEVSHWKEKEINGT--ERRLQGGCPLTPRETSLLLRALEFPSSSRIYLVAGE 501

BLAST of Lag0031186 vs. ExPASy TrEMBL
Match: A0A6J1F5I4 (O-fucosyltransferase family protein OS=Cucurbita moschata OX=3662 GN=LOC111442337 PE=3 SV=1)

HSP 1 Score: 818.1 bits (2112), Expect = 2.0e-233
Identity = 413/444 (93.02%), Postives = 428/444 (96.40%), Query Frame = 0

Query: 1   MAKKNSNSKKQCYISVPSQIINSLSSSSLQSLLDSPKKSSKTYNKYLSLSTFRSSKKLWL 60
           MAKKNSNSKKQCYISVPSQIINSLSSSSLQSLLDSPKKSSK Y+KYLSL T+R+SKKLWL
Sbjct: 1   MAKKNSNSKKQCYISVPSQIINSLSSSSLQSLLDSPKKSSKHYDKYLSLFTYRASKKLWL 60

Query: 61  LAIFLFGLLGMLKLSFNLGPLIAYVSYPCFTSQSQSQGFVSNGFLKSGVIDTEKENVLQD 120
           LAIFLFG LGMLKL+FNL PLIAYVSYPCFTSQSQSQG VSNG LKSGVID E+EN L+D
Sbjct: 61  LAIFLFGFLGMLKLTFNLDPLIAYVSYPCFTSQSQSQGLVSNGVLKSGVIDRERENALKD 120

Query: 121 EIVNDSVQSLRPISNGVLKSDEGFGEENEQQEFWKQPDGLGYKPCLDFSQEYKKSTTGIV 180
           E +NDS+QSLRPISNGVLKSDEGFG+ NE QEFWKQPDGLGYKPCLDFSQEYKK+TTG++
Sbjct: 121 ESMNDSIQSLRPISNGVLKSDEGFGDGNEPQEFWKQPDGLGYKPCLDFSQEYKKTTTGVI 180

Query: 181 SERTKYLMVVVSGGMNQQRNQIVDAVVIARILGAALVVPILQVNVIWGDESEFSDIFDLE 240
           SERTKYLMVVVSGGMNQQRNQIVDAVVIARILGAALVVPI QVNVIWGDESEFSDIFDLE
Sbjct: 181 SERTKYLMVVVSGGMNQQRNQIVDAVVIARILGAALVVPIFQVNVIWGDESEFSDIFDLE 240

Query: 241 HFKSVLADDVHVVSSLPSTHLMTRPVEEKSPPHQVSPSWIRSRYLRKLRREGVLLLRGLD 300
           HFK+VLADDVH+VSSLPSTHLMTRPVEEKS PH VSPSWIRSRYLRKLRREGVLLLRGLD
Sbjct: 241 HFKNVLADDVHIVSSLPSTHLMTRPVEEKSTPHHVSPSWIRSRYLRKLRREGVLLLRGLD 300

Query: 301 SRLSKDLPSDLQKLRCKVAFHALRFAPPIVELGNKLTERMRSKGPYLALHLRMEKDVWVR 360
           SRLSKDLPSDLQKLRCKVAFHALRFAPPIVELGNKLTERMRSKGPYLALHLRMEKDVWVR
Sbjct: 301 SRLSKDLPSDLQKLRCKVAFHALRFAPPIVELGNKLTERMRSKGPYLALHLRMEKDVWVR 360

Query: 361 TGCLPGLSPEYDEMINNERIRRPELLTARSNMSYHDRKLAGLCPLNAYEVMRLLKALGAP 420
           TGCLPGLSPEYDEMINNERIRRPELLTARSNMSYHDRKLAGLCPLNAYEVMRLLKALGAP
Sbjct: 361 TGCLPGLSPEYDEMINNERIRRPELLTARSNMSYHDRKLAGLCPLNAYEVMRLLKALGAP 420

Query: 421 RDTRIYWAGGEPLGGKEALQPLTR 445
            DTRIYWAGG+PLGGKEALQPL R
Sbjct: 421 GDTRIYWAGGQPLGGKEALQPLIR 444

BLAST of Lag0031186 vs. ExPASy TrEMBL
Match: A0A6J1J5X6 (O-fucosyltransferase family protein OS=Cucurbita maxima OX=3661 GN=LOC111482060 PE=3 SV=1)

HSP 1 Score: 815.1 bits (2104), Expect = 1.7e-232
Identity = 411/444 (92.57%), Postives = 427/444 (96.17%), Query Frame = 0

Query: 1   MAKKNSNSKKQCYISVPSQIINSLSSSSLQSLLDSPKKSSKTYNKYLSLSTFRSSKKLWL 60
           MAKKNSNSKKQCYISVPSQIINSLSSSSLQSLLDSPKKSSK Y+KYLSL T+R+SKKLWL
Sbjct: 1   MAKKNSNSKKQCYISVPSQIINSLSSSSLQSLLDSPKKSSKHYDKYLSLFTYRASKKLWL 60

Query: 61  LAIFLFGLLGMLKLSFNLGPLIAYVSYPCFTSQSQSQGFVSNGFLKSGVIDTEKENVLQD 120
           LAIFLFG LGMLKL+FNL PL AYVSYPCF SQSQSQG VSNG LKSGV+D E+EN L+D
Sbjct: 61  LAIFLFGFLGMLKLTFNLDPLFAYVSYPCFMSQSQSQGLVSNGALKSGVMDRERENALKD 120

Query: 121 EIVNDSVQSLRPISNGVLKSDEGFGEENEQQEFWKQPDGLGYKPCLDFSQEYKKSTTGIV 180
           E +NDS+QSLRPISNGVLKSDEGFG+ NEQQEFWKQPDGLGYKPCLDFSQEYKK+TTG++
Sbjct: 121 ESMNDSIQSLRPISNGVLKSDEGFGDGNEQQEFWKQPDGLGYKPCLDFSQEYKKTTTGVI 180

Query: 181 SERTKYLMVVVSGGMNQQRNQIVDAVVIARILGAALVVPILQVNVIWGDESEFSDIFDLE 240
           SERTKYLMVVVSGGMNQQRNQIVDAVVIARILGAALVVPI QVNVIWGDESEFSDIFDLE
Sbjct: 181 SERTKYLMVVVSGGMNQQRNQIVDAVVIARILGAALVVPIFQVNVIWGDESEFSDIFDLE 240

Query: 241 HFKSVLADDVHVVSSLPSTHLMTRPVEEKSPPHQVSPSWIRSRYLRKLRREGVLLLRGLD 300
           HFK+VLADDVH+VSSLPSTHLMTRPVEEKS PH VSPSWIRSRYLRKLRREGVLLLRGLD
Sbjct: 241 HFKNVLADDVHIVSSLPSTHLMTRPVEEKSTPHHVSPSWIRSRYLRKLRREGVLLLRGLD 300

Query: 301 SRLSKDLPSDLQKLRCKVAFHALRFAPPIVELGNKLTERMRSKGPYLALHLRMEKDVWVR 360
           SRLSKDLPSDLQKLRCKVAFHALRFAPPIVELGNKLTERMRSKGPYLALHLRMEKDVWVR
Sbjct: 301 SRLSKDLPSDLQKLRCKVAFHALRFAPPIVELGNKLTERMRSKGPYLALHLRMEKDVWVR 360

Query: 361 TGCLPGLSPEYDEMINNERIRRPELLTARSNMSYHDRKLAGLCPLNAYEVMRLLKALGAP 420
           TGCLPGLSPEYDEMINNERIRRPELLTARSNMSYHDRKLAGLCPLNAYEVMRLLKALGAP
Sbjct: 361 TGCLPGLSPEYDEMINNERIRRPELLTARSNMSYHDRKLAGLCPLNAYEVMRLLKALGAP 420

Query: 421 RDTRIYWAGGEPLGGKEALQPLTR 445
            DTRIYWAGG+PLGGKEALQPL R
Sbjct: 421 GDTRIYWAGGQPLGGKEALQPLIR 444

BLAST of Lag0031186 vs. ExPASy TrEMBL
Match: A0A0A0L9W4 (O-fucosyltransferase family protein OS=Cucumis sativus OX=3659 GN=Csa_3G702070 PE=3 SV=1)

HSP 1 Score: 802.0 bits (2070), Expect = 1.5e-228
Identity = 409/443 (92.33%), Postives = 422/443 (95.26%), Query Frame = 0

Query: 1   MAKKNSNSKKQCYISVPSQIINSLSSSSLQSLLDSPKKSSKTYNKYLSLSTFRSSKKLWL 60
           MAKKNSNSKKQ YISVPSQIINSLSSSSLQSLLDSPKKSSK YNK+LSLST+RSS KLWL
Sbjct: 1   MAKKNSNSKKQAYISVPSQIINSLSSSSLQSLLDSPKKSSKNYNKFLSLSTYRSSNKLWL 60

Query: 61  LAIFLFGLLGMLKLSFNLGPLIAYVSYPCFTSQSQSQGFVSNGFLKSGVIDTEKENVLQD 120
           LAIFLFGL GMLKL+FN  PLI YVSYPCFTSQ QSQG VSNG LKSG+ID ++EN L+D
Sbjct: 61  LAIFLFGLFGMLKLTFNPNPLIPYVSYPCFTSQWQSQGLVSNGVLKSGLIDRDRENALKD 120

Query: 121 EIVNDSVQSLRPISNGVLKSDEGFGEENEQQEFWKQPDGLGYKPCLDFSQEYKKSTTGIV 180
           E+ +DS QSLRPIS GVLKSDEG    NEQ EFWKQPDGLGYKPCLDFS+EYKKSTTGIV
Sbjct: 121 EVTSDSFQSLRPISTGVLKSDEG----NEQGEFWKQPDGLGYKPCLDFSEEYKKSTTGIV 180

Query: 181 SERTKYLMVVVSGGMNQQRNQIVDAVVIARILGAALVVPILQVNVIWGDESEFSDIFDLE 240
           SERTKYLMVVVSGGMNQQRNQIVDAVVIARILGAALVVPILQVNVIWGDESEFSDIFDLE
Sbjct: 181 SERTKYLMVVVSGGMNQQRNQIVDAVVIARILGAALVVPILQVNVIWGDESEFSDIFDLE 240

Query: 241 HFKSVLADDVHVVSSLPSTHLMTRPVEEKSPPHQVSPSWIRSRYLRKLRREGVLLLRGLD 300
           HFK+VLADDVH+VSSLPSTHLMTRPVEEKSPPH VSPSWIRSRYLRKLRREGVLLLRGLD
Sbjct: 241 HFKNVLADDVHIVSSLPSTHLMTRPVEEKSPPHHVSPSWIRSRYLRKLRREGVLLLRGLD 300

Query: 301 SRLSKDLPSDLQKLRCKVAFHALRFAPPIVELGNKLTERMRSKGPYLALHLRMEKDVWVR 360
           SRLSKDLPSDLQKLRCKVAFHALRFAPPIVELGNKLTERMRSKGPYLALHLRMEKDVWVR
Sbjct: 301 SRLSKDLPSDLQKLRCKVAFHALRFAPPIVELGNKLTERMRSKGPYLALHLRMEKDVWVR 360

Query: 361 TGCLPGLSPEYDEMINNERIRRPELLTARSNMSYHDRKLAGLCPLNAYEVMRLLKALGAP 420
           TGCLPGLSPEYDEMINNERIRRPELLTARSNMSYHDRKLAGLCPLNAYEVMRLLKALGAP
Sbjct: 361 TGCLPGLSPEYDEMINNERIRRPELLTARSNMSYHDRKLAGLCPLNAYEVMRLLKALGAP 420

Query: 421 RDTRIYWAGGEPLGGKEALQPLT 444
           RDTRIYWAGG+PLGGKEALQPLT
Sbjct: 421 RDTRIYWAGGQPLGGKEALQPLT 439

BLAST of Lag0031186 vs. ExPASy TrEMBL
Match: A0A6J1GBZ8 (O-fucosyltransferase family protein OS=Cucurbita moschata OX=3662 GN=LOC111452776 PE=3 SV=1)

HSP 1 Score: 795.8 bits (2054), Expect = 1.0e-226
Identity = 407/445 (91.46%), Postives = 429/445 (96.40%), Query Frame = 0

Query: 1   MAKKNSNSKKQCYISVPSQIINSLSSSSLQSLLDSPKKSSKTYNKYLSLSTFRSSKKLWL 60
           MAKKNSNSKKQCYISVPSQIINSLS+SSLQSLLDSPKKSSKTYNKYLSLST+R+SKKLWL
Sbjct: 1   MAKKNSNSKKQCYISVPSQIINSLSTSSLQSLLDSPKKSSKTYNKYLSLSTYRNSKKLWL 60

Query: 61  LAIFLFGLLGMLKLSFNLGPLIAYVSYPCFTSQS--QSQGFVSNGFLKSGVIDTEKENVL 120
           LAIFLFGLLGMLKL+FNL PLI  VSYPCF+SQS  QSQ  VSNG LKSGVID EKENV+
Sbjct: 61  LAIFLFGLLGMLKLNFNLNPLI--VSYPCFSSQSLLQSQSLVSNGVLKSGVIDREKENVV 120

Query: 121 QDEIVNDSVQSLRPISNGVLKSDEGFGEENEQQEFWKQPDGLGYKPCLDFSQEYKKSTTG 180
           +D I+NDSVQSL+PISNGVLKS+EGF E +E+ EFWKQPDGLGYKPCLDFS++Y+KSTTG
Sbjct: 121 EDVIMNDSVQSLKPISNGVLKSNEGFEEGSEEVEFWKQPDGLGYKPCLDFSEDYRKSTTG 180

Query: 181 IVSERTKYLMVVVSGGMNQQRNQIVDAVVIARILGAALVVPILQVNVIWGDESEFSDIFD 240
           IVSER+KYLMVVVSGGMNQQRNQIVDAVVIARILGAALVVPILQVNVIWGDESEFSDIFD
Sbjct: 181 IVSERSKYLMVVVSGGMNQQRNQIVDAVVIARILGAALVVPILQVNVIWGDESEFSDIFD 240

Query: 241 LEHFKSVLADDVHVVSSLPSTHLMTRPVEEKSPPHQVSPSWIRSRYLRKLRREGVLLLRG 300
           LEHFK+VLADDVH+VSSLPSTHLMTRPVE+KSPPH VSPSWIRSRYLRKLRREGVLLLRG
Sbjct: 241 LEHFKNVLADDVHIVSSLPSTHLMTRPVEQKSPPHHVSPSWIRSRYLRKLRREGVLLLRG 300

Query: 301 LDSRLSKDLPSDLQKLRCKVAFHALRFAPPIVELGNKLTERMRSKGPYLALHLRMEKDVW 360
           LDSRLSKDLPSDLQKLRCKVAF ALRFAPPIVELGNKLTERMRSKG YLALHLRMEKDVW
Sbjct: 301 LDSRLSKDLPSDLQKLRCKVAFRALRFAPPIVELGNKLTERMRSKGQYLALHLRMEKDVW 360

Query: 361 VRTGCLPGLSPEYDEMINNERIRRPELLTARSNMSYHDRKLAGLCPLNAYEVMRLLKALG 420
           VRTGCLPGLSP+YDEMINNERI+RPELLTARSNMSYHDRKLAGLCPLNAYEVMRLLKALG
Sbjct: 361 VRTGCLPGLSPKYDEMINNERIQRPELLTARSNMSYHDRKLAGLCPLNAYEVMRLLKALG 420

Query: 421 APRDTRIYWAGGEPLGGKEALQPLT 444
           APRDTRIYWAGG+PLGGKEALQPLT
Sbjct: 421 APRDTRIYWAGGQPLGGKEALQPLT 443

BLAST of Lag0031186 vs. ExPASy TrEMBL
Match: A0A5D3CKB7 (O-fucosyltransferase family protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold25G00110 PE=3 SV=1)

HSP 1 Score: 795.4 bits (2053), Expect = 1.4e-226
Identity = 406/443 (91.65%), Postives = 419/443 (94.58%), Query Frame = 0

Query: 1   MAKKNSNSKKQCYISVPSQIINSLSSSSLQSLLDSPKKSSKTYNKYLSLSTFRSSKKLWL 60
           MAKKNSNSKKQ YISVPSQIINSLSSSSLQSLLDSPKKSSK YNK+ SLST+RSS KLWL
Sbjct: 1   MAKKNSNSKKQAYISVPSQIINSLSSSSLQSLLDSPKKSSKNYNKFFSLSTYRSSNKLWL 60

Query: 61  LAIFLFGLLGMLKLSFNLGPLIAYVSYPCFTSQSQSQGFVSNGFLKSGVIDTEKENVLQD 120
           LAIFLFGL GMLKL+FN  PL  YVSYPCFTSQ QSQG VSNG LKSG+ID ++EN L+D
Sbjct: 61  LAIFLFGLFGMLKLTFNPNPLFPYVSYPCFTSQWQSQGLVSNGVLKSGLIDRDRENALKD 120

Query: 121 EIVNDSVQSLRPISNGVLKSDEGFGEENEQQEFWKQPDGLGYKPCLDFSQEYKKSTTGIV 180
           E+ +DS QSLR IS GVLKSDEG    NEQ EFWKQPDGLGYKPCLDFS+EYKKSTTGIV
Sbjct: 121 EVTSDSFQSLRRISTGVLKSDEG----NEQGEFWKQPDGLGYKPCLDFSEEYKKSTTGIV 180

Query: 181 SERTKYLMVVVSGGMNQQRNQIVDAVVIARILGAALVVPILQVNVIWGDESEFSDIFDLE 240
           SERTKYLMVVVSGGMNQQRNQIVDAVVIARILGAALVVPILQVNVIWGDESEFSDIFDLE
Sbjct: 181 SERTKYLMVVVSGGMNQQRNQIVDAVVIARILGAALVVPILQVNVIWGDESEFSDIFDLE 240

Query: 241 HFKSVLADDVHVVSSLPSTHLMTRPVEEKSPPHQVSPSWIRSRYLRKLRREGVLLLRGLD 300
           HFK+VLADDVH+VSSLPSTHLMTRPVEEKSPPH VSPSWIRSRYLRKLRREGVLLLRGLD
Sbjct: 241 HFKNVLADDVHIVSSLPSTHLMTRPVEEKSPPHHVSPSWIRSRYLRKLRREGVLLLRGLD 300

Query: 301 SRLSKDLPSDLQKLRCKVAFHALRFAPPIVELGNKLTERMRSKGPYLALHLRMEKDVWVR 360
           SRLSKDLPSDLQKLRCKVAFHALRFAPPIVELGNKLTERMRSKGPYLALHLRMEKDVWVR
Sbjct: 301 SRLSKDLPSDLQKLRCKVAFHALRFAPPIVELGNKLTERMRSKGPYLALHLRMEKDVWVR 360

Query: 361 TGCLPGLSPEYDEMINNERIRRPELLTARSNMSYHDRKLAGLCPLNAYEVMRLLKALGAP 420
           TGCLPGLSPEYDEMINNERIRRPELLTARSNMSYHDRKLAGLCPLNAYEVMRLLKALGAP
Sbjct: 361 TGCLPGLSPEYDEMINNERIRRPELLTARSNMSYHDRKLAGLCPLNAYEVMRLLKALGAP 420

Query: 421 RDTRIYWAGGEPLGGKEALQPLT 444
           RDTRIYWAGG+PLGGKEALQPLT
Sbjct: 421 RDTRIYWAGGQPLGGKEALQPLT 439

BLAST of Lag0031186 vs. TAIR 10
Match: AT2G44500.1 (O-fucosyltransferase family protein )

HSP 1 Score: 548.9 bits (1413), Expect = 4.3e-156
Identity = 292/454 (64.32%), Postives = 345/454 (75.99%), Query Frame = 0

Query: 4   KNSNS-----KKQCYISVPSQIINSLSSSSLQSLLDSPKKSSKTYNKYLSLSTFRSSKKL 63
           KNSNS     KK  YISVPSQIINSLSSSSLQSLL SPKKSS++ N++   S    + ++
Sbjct: 5   KNSNSNSFNKKKVSYISVPSQIINSLSSSSLQSLLVSPKKSSRSTNRF---SFSYRNPRI 64

Query: 64  WLLAIFLFGLLGMLKLSFNLGPL-IAYVSYPCFTSQSQ-----SQGFVSN-GFLKSGVID 123
           W   +FL  L GMLKL FN+ P+ + +  YPC T+Q        Q   S+ G  +  ++ 
Sbjct: 65  WFFTLFLVSLFGMLKLGFNVDPISLPFSRYPCSTTQQPLSFDGEQNAASHLGLAQEPILS 124

Query: 124 TEKENVLQDEIVNDSVQSLRPISNGVLKSDEGFGEE-NEQQEFWKQPDGLGYKPCLDFSQ 183
           T   N                 SN +++ + G  E    + +FWKQPDGLG+KPCL F+ 
Sbjct: 125 TGSSN-----------------SNAIIQLNGGKNETLLTEGDFWKQPDGLGFKPCLGFTS 184

Query: 184 EYKKSTTGIVSERTKYLMVVVSGGMNQQRNQIVDAVVIARILGAALVVPILQVNVIWGDE 243
           +Y+K +  I+  R KYL+VVVSGGMNQQRNQIVDAVVIARILGA+LVVP+LQVNVIWGDE
Sbjct: 185 QYRKDSNSILKNRWKYLLVVVSGGMNQQRNQIVDAVVIARILGASLVVPVLQVNVIWGDE 244

Query: 244 SEFSDIFDLEHFKSVLADDVHVVSSLPSTHLMTRPVEEKSPPHQVSPSWIRSRYLRKLRR 303
           SEF+DIFDLEHFK VLADDVH+VSSLPSTH+MTRPVEEK  P   SP WIR+ YL+++ R
Sbjct: 245 SEFADIFDLEHFKDVLADDVHIVSSLPSTHVMTRPVEEKRTPLHASPQWIRAHYLKRINR 304

Query: 304 EGVLLLRGLDSRLSKDLPSDLQKLRCKVAFHALRFAPPIVELGNKLTERMRSKGPYLALH 363
           E VLLLRGLDSRLSKDLPSDLQKLRCKVAF ALRF+P I+ELGNKL  RMR++G YL+LH
Sbjct: 305 ERVLLLRGLDSRLSKDLPSDLQKLRCKVAFQALRFSPRILELGNKLASRMRNQGQYLSLH 364

Query: 364 LRMEKDVWVRTGCLPGLSPEYDEMINNERIRRPELLTARSNMSYHDRKLAGLCPLNAYEV 423
           LRMEKDVWVRTGCLPGL+PEYDE++N+ER R PELLT RSNM+YH+RKLAGLCPL A EV
Sbjct: 365 LRMEKDVWVRTGCLPGLTPEYDEIVNSERERHPELLTGRSNMTYHERKLAGLCPLTALEV 424

Query: 424 MRLLKALGAPRDTRIYWAGGEPLGGKEALQPLTR 445
            RLLKAL AP+D RIYWAGGEPLGGKE L+PLT+
Sbjct: 425 TRLLKALEAPKDARIYWAGGEPLGGKEVLEPLTK 438

BLAST of Lag0031186 vs. TAIR 10
Match: AT3G07900.1 (O-fucosyltransferase family protein )

HSP 1 Score: 498.0 bits (1281), Expect = 8.7e-141
Identity = 277/454 (61.01%), Postives = 334/454 (73.57%), Query Frame = 0

Query: 4   KNSNSKKQ-CYISVPSQIINSLSSSSLQSLLDSPKKSSKTYNKYLSLSTFRSSKKLWLLA 63
           KNSN K +  YISVP+QIINS+SSSSL   LD+      T   + +L     + KLW  +
Sbjct: 7   KNSNKKNRVSYISVPAQIINSVSSSSLHKFLDNKSSKKNTSKFFFNL----RNPKLWAFS 66

Query: 64  IFLFGLLGMLKLSFNLGPLIAYVSYPCFTSQSQSQGFVSNGFLKSGV-IDTEKENVLQDE 123
           +FL  +LG   +S  LG  +++       SQ QS    SNG  KS +     + +  Q E
Sbjct: 67  LFLLSILG---ISLRLGLCLSHFGSGDHESQLQSSD--SNGSPKSHMGFAYIRSSTTQVE 126

Query: 124 IVNDSVQSLRPISNGVLKSD------------EGFGEENEQQEFWKQPDGLGYKPCLDFS 183
           I N   +SL     GV K++             G G +++  +FWKQPDGLGYKPCLDFS
Sbjct: 127 ISNAKDRSL---DMGVEKNETFGGHQSHLIIPNGNGHDDKNYDFWKQPDGLGYKPCLDFS 186

Query: 184 QEYKKSTTGIVSERTKYLMVVVSGGMNQQRNQIVDAVVIARILGAALVVPILQVNVIWGD 243
            EY++ +  I+ ER KYLMVVVSGG+NQQ+ QIVDAVVIARILGA LVVPILQ+N+IWGD
Sbjct: 187 IEYRRESKKILVERRKYLMVVVSGGLNQQKIQIVDAVVIARILGAVLVVPILQINLIWGD 246

Query: 244 ESEFSDIFDLEHFKSVLADDVHVVSSLPSTHLMTRPVEEKSPPHQVSPSWIRSRYLRKLR 303
           ESEFSDIFDLE FKSVLA+DV +VS LP++ +MTRP E+ S P   SP WIRS Y ++  
Sbjct: 247 ESEFSDIFDLEQFKSVLANDVKIVSLLPASKVMTRPSEDGSMPFNASPQWIRSHYPKRFN 306

Query: 304 REGVLLLRGLDSRLSKDLPSDLQKLRCKVAFHALRFAPPIVELGNKLTERMRSKGPYLAL 363
           REGVLLLR LDSRLSKDLPSDLQKLRCKVAF AL+F+P ++E+G KL ERMRSKGPY+AL
Sbjct: 307 REGVLLLRRLDSRLSKDLPSDLQKLRCKVAFEALKFSPRVMEMGTKLAERMRSKGPYIAL 366

Query: 364 HLRMEKDVWVRTGCLPGLSPEYDEMINNERIRRPELLTARSNMSYHDRKLAGLCPLNAYE 423
           HLRMEKDVWVRTGCL GLS +YDE++N ERI+RPELLTA+S+M+ ++RKLAGLCPLNA E
Sbjct: 367 HLRMEKDVWVRTGCLSGLSSKYDEIVNIERIKRPELLTAKSSMTSNERKLAGLCPLNAKE 426

Query: 424 VMRLLKALGAPRDTRIYWAGGEPLGGKEALQPLT 444
           V RLL+ALGAPRD RIYWAGGEPLGGKEAL+PLT
Sbjct: 427 VTRLLRALGAPRDARIYWAGGEPLGGKEALKPLT 448

BLAST of Lag0031186 vs. TAIR 10
Match: AT2G44500.2 (O-fucosyltransferase family protein )

HSP 1 Score: 492.7 bits (1267), Expect = 3.6e-139
Identity = 266/422 (63.03%), Postives = 316/422 (74.88%), Query Frame = 0

Query: 4   KNSNS-----KKQCYISVPSQIINSLSSSSLQSLLDSPKKSSKTYNKYLSLSTFRSSKKL 63
           KNSNS     KK  YISVPSQIINSLSSSSLQSLL SPKKSS++ N++   S    + ++
Sbjct: 5   KNSNSNSFNKKKVSYISVPSQIINSLSSSSLQSLLVSPKKSSRSTNRF---SFSYRNPRI 64

Query: 64  WLLAIFLFGLLGMLKLSFNLGPL-IAYVSYPCFTSQSQ-----SQGFVSN-GFLKSGVID 123
           W   +FL  L GMLKL FN+ P+ + +  YPC T+Q        Q   S+ G  +  ++ 
Sbjct: 65  WFFTLFLVSLFGMLKLGFNVDPISLPFSRYPCSTTQQPLSFDGEQNAASHLGLAQEPILS 124

Query: 124 TEKENVLQDEIVNDSVQSLRPISNGVLKSDEGFGEE-NEQQEFWKQPDGLGYKPCLDFSQ 183
           T   N                 SN +++ + G  E    + +FWKQPDGLG+KPCL F+ 
Sbjct: 125 TGSSN-----------------SNAIIQLNGGKNETLLTEGDFWKQPDGLGFKPCLGFTS 184

Query: 184 EYKKSTTGIVSERTKYLMVVVSGGMNQQRNQIVDAVVIARILGAALVVPILQVNVIWGDE 243
           +Y+K +  I+  R KYL+VVVSGGMNQQRNQIVDAVVIARILGA+LVVP+LQVNVIWGDE
Sbjct: 185 QYRKDSNSILKNRWKYLLVVVSGGMNQQRNQIVDAVVIARILGASLVVPVLQVNVIWGDE 244

Query: 244 SEFSDIFDLEHFKSVLADDVHVVSSLPSTHLMTRPVEEKSPPHQVSPSWIRSRYLRKLRR 303
           SEF+DIFDLEHFK VLADDVH+VSSLPSTH+MTRPVEEK  P   SP WIR+ YL+++ R
Sbjct: 245 SEFADIFDLEHFKDVLADDVHIVSSLPSTHVMTRPVEEKRTPLHASPQWIRAHYLKRINR 304

Query: 304 EGVLLLRGLDSRLSKDLPSDLQKLRCKVAFHALRFAPPIVELGNKLTERMRSKGPYLALH 363
           E VLLLRGLDSRLSKDLPSDLQKLRCKVAF ALRF+P I+ELGNKL  RMR++G YL+LH
Sbjct: 305 ERVLLLRGLDSRLSKDLPSDLQKLRCKVAFQALRFSPRILELGNKLASRMRNQGQYLSLH 364

Query: 364 LRMEKDVWVRTGCLPGLSPEYDEMINNERIRRPELLTARSNMSYHDRKLAGLCPLNAYEV 413
           LRMEKDVWVRTGCLPGL+PEYDE++N+ER R PELLT RSNM+YH+RKLAGLCPL A EV
Sbjct: 365 LRMEKDVWVRTGCLPGLTPEYDEIVNSERERHPELLTGRSNMTYHERKLAGLCPLTALEV 406

BLAST of Lag0031186 vs. TAIR 10
Match: AT5G63390.1 (O-fucosyltransferase family protein )

HSP 1 Score: 413.7 bits (1062), Expect = 2.2e-115
Identity = 207/363 (57.02%), Postives = 259/363 (71.35%), Query Frame = 0

Query: 82  IAYVSYPCFTSQSQSQGFVSNGFLKSGVIDTEKENVLQDEIVNDSVQSLRPISNGVLKSD 141
           I+++++   T  S      S   LK  + D +   +L    V+    +L P+     +  
Sbjct: 67  ISFLTFSLDTGSSTCVSTSSASSLKFFISDVDHRQILTSLAVS-GASTLFPLP---ARGG 126

Query: 142 EGFGEENEQQEFWKQPDGLGYKPCLDFSQEYKKSTTGIVSERTKYLMVVVSGGMNQQRNQ 201
                  E++EFWKQP+G GYKPCLDFS EYKK +  +  E+ ++L+VVVSGG+NQQRNQ
Sbjct: 127 HSGNMTEEEKEFWKQPNGEGYKPCLDFSLEYKKKSASVSKEKKRFLVVVVSGGLNQQRNQ 186

Query: 202 IVDAVVIARILGAALVVPILQVNVIWGDESEFSDIFDLEHFKSVLADDVHVVSSLPSTHL 261
           IVDAVVIA IL AALVVP+LQVN +WGDESEFSD+FD+EHFK  L  DV +VSSLPSTHL
Sbjct: 187 IVDAVVIAMILEAALVVPVLQVNRVWGDESEFSDLFDVEHFKKTLRSDVRIVSSLPSTHL 246

Query: 262 MTRPVEEKSPPHQVSPSWIRSRYLRKLRREGVLLLRGLDSRLSKDLPSDLQKLRCKVAFH 321
           M+R   E   P  VSP WIR++Y ++L  EG+L+L+GLDS+L+K+LP DLQKLRCKVAFH
Sbjct: 247 MSRQTIENQIPWDVSPVWIRAKYFKQLNEEGLLVLKGLDSKLAKNLPPDLQKLRCKVAFH 306

Query: 322 ALRFAPPIVELGNKLTERMRSKGPYLALHLRMEKDVWVRTGCLPGLSPEYDEMINNERIR 381
           ALRFA PI  LGNKLT RM  +GPY+ALHLR+EKDVWVRTGCL GL  E+D +I   R  
Sbjct: 307 ALRFAAPIENLGNKLTRRMWIEGPYIALHLRLEKDVWVRTGCLTGLGSEFDRIIAETRTS 366

Query: 382 RPELLTARSNMSYHDRKLAGLCPLNAYEVMRLLKALGAPRDTRIYWAGGEPLGGKEALQP 441
           +P  LT R N++Y +R+LAG CPLN YE+ RLLKALGAP +  IY AGGEP GG  AL+P
Sbjct: 367 QPRYLTGRLNLTYTERRLAGFCPLNVYEIARLLKALGAPSNASIYIAGGEPFGGSRALEP 425

Query: 442 LTR 445
           L +
Sbjct: 427 LAK 425

BLAST of Lag0031186 vs. TAIR 10
Match: AT5G35570.1 (O-fucosyltransferase family protein )

HSP 1 Score: 191.8 bits (486), Expect = 1.3e-48
Identity = 117/303 (38.61%), Postives = 165/303 (54.46%), Query Frame = 0

Query: 137 VLKSDEGFGEENEQQEF---WKQPDGLGYKPCLDFSQEYKKSTTGIVSERTKYLMVVVSG 196
           V+ + +  G++N   EF   W +P+   +  C+D S+  KK    + +    YL++  +G
Sbjct: 206 VMVASDVVGDQNSVVEFSGVWAKPESGNFSRCIDSSRSRKK----LGANTNGYLLINANG 265

Query: 197 GMNQQRNQIVDAVVIARILGAALVVPILQVNVIWGDESEFSDIFDLEHFKSVLADDVHVV 256
           G+NQ R  I D V +A+I+ A LV+P L  +  W D+S F D+FD +HF   L DD+H+V
Sbjct: 266 GLNQMRFGICDMVAVAKIMKATLVLPSLDHSSYWADDSGFKDLFDWQHFIEELKDDIHIV 325

Query: 257 SSLPSTHLMTRPVEEKSPPHQVSPSWIRSRYLRKLRREGVLLLRGLDSRL-SKDLPSDLQ 316
             LPS      P   K+P       + +   L  L++  V+ L   DSRL + DLP  +Q
Sbjct: 326 EMLPSELAGIEPF-VKTPISWSKVGYYKKEVLPLLKQHIVMYLTHTDSRLANNDLPDSVQ 385

Query: 317 KLRCKVAFHALRFAPPIVELGNKLTERMR-SKGPYLALHLRMEKDVWVRTGCLPGLSPEY 376
           KLRC+V + AL+++ PI ELGN L  RMR  +GPYLALHLR EKD+   TGC   L+ E 
Sbjct: 386 KLRCRVNYRALKYSAPIEELGNVLVSRMRQDRGPYLALHLRYEKDMLAFTGCSHSLTAEE 445

Query: 377 DEMINNERIRRPELLTARSNMSYHDRKLAGLCPLNAYEVMRLLKALGAPRDTRIYWAGGE 435
           DE +   R           N +  +R+L G CPL   E   LL+AL  P  +RIY   GE
Sbjct: 446 DEELRQMRYEVSHWKEKEINGT--ERRLQGGCPLTPRETSLLLRALEFPSSSRIYLVAGE 501

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022935474.14.1e-23393.02O-fucosyltransferase 20-like [Cucurbita moschata] >KAG7018100.1 hypothetical pro... [more]
KAG6581598.14.1e-23393.02O-fucosyltransferase 20, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_038906082.11.5e-23293.47O-fucosyltransferase 20 [Benincasa hispida][more]
XP_022983465.13.4e-23292.57O-fucosyltransferase 20-like [Cucurbita maxima][more]
XP_023528632.13.2e-23091.89O-fucosyltransferase 20-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
O648846.0e-15564.32O-fucosyltransferase 20 OS=Arabidopsis thaliana OX=3702 GN=OFUT20 PE=1 SV=1[more]
A0A0B5GR441.9e-14863.72O-fucosyltransferase 20 OS=Brassica napus OX=3708 GN=OFUT20 PE=2 SV=2[more]
Q9SFC41.2e-13961.01O-fucosyltransferase 24 OS=Arabidopsis thaliana OX=3702 GN=OFUT24 PE=2 SV=1[more]
Q9FMW33.0e-11457.02O-fucosyltransferase 37 OS=Arabidopsis thaliana OX=3702 GN=OFUT37 PE=2 SV=1[more]
Q94BY41.9e-4738.61O-fucosyltransferase 35 OS=Arabidopsis thaliana OX=3702 GN=OFUT35 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1F5I42.0e-23393.02O-fucosyltransferase family protein OS=Cucurbita moschata OX=3662 GN=LOC11144233... [more]
A0A6J1J5X61.7e-23292.57O-fucosyltransferase family protein OS=Cucurbita maxima OX=3661 GN=LOC111482060 ... [more]
A0A0A0L9W41.5e-22892.33O-fucosyltransferase family protein OS=Cucumis sativus OX=3659 GN=Csa_3G702070 P... [more]
A0A6J1GBZ81.0e-22691.46O-fucosyltransferase family protein OS=Cucurbita moschata OX=3662 GN=LOC11145277... [more]
A0A5D3CKB71.4e-22691.65O-fucosyltransferase family protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5... [more]
Match NameE-valueIdentityDescription
AT2G44500.14.3e-15664.32O-fucosyltransferase family protein [more]
AT3G07900.18.7e-14161.01O-fucosyltransferase family protein [more]
AT2G44500.23.6e-13963.03O-fucosyltransferase family protein [more]
AT5G63390.12.2e-11557.02O-fucosyltransferase family protein [more]
AT5G35570.11.3e-4838.61O-fucosyltransferase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR024709Putative O-fucosyltransferase, plantPIRSFPIRSF009360UCP009360coord: 20..452
e-value: 8.9E-118
score: 392.3
IPR019378GDP-fucose protein O-fucosyltransferasePFAMPF10250O-FucTcoord: 186..444
e-value: 1.0E-42
score: 147.1
NoneNo IPR availablePANTHERPTHR31741:SF66O-FUCOSYLTRANSFERASE 20coord: 13..453
NoneNo IPR availablePANTHERPTHR31741OS02G0726500 PROTEIN-RELATEDcoord: 13..453

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0031186.1Lag0031186.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006004 fucose metabolic process
cellular_component GO:0005737 cytoplasm
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0016757 glycosyltransferase activity