Lag0031126 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0031126
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	acyl-CoA--sterol O-acyltransferase 1-like
Location	chr11: 5055507 .. 5056562 (-)
RNA-Seq Expression	Lag0031126
Synteny	Lag0031126

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGGGAGAGATTTTCAACTTCGTCAAGGTCTGGTTAGCAGTCTGTATCTCTCTCTGTTACTGCCACGCCGCCGGAAAACTCCTCCCTCCCGGCGCCGGCCGCTTCCTCGCCGTCGCCCCCGTCGTCGCCCTCTTCCTCCTCCTCCCTCTGCAACTCACCTCCATCCACCTCGGCGGCTCCACCTCCTTCTTCGTCGCTTGGCTCGCTAACTTCAAGCTCCTCCTCTTCGCCGCTGGCGACGGCCCTCTCTCCGCCGCCCCGCCGCTCTCTCTCCGCCGCTTCCTCGCACTCTCCTGCTTGCCGATCAAGATCCTCCAAAACCCCTCGGAGATATCGAGCCCTAACAATCGCGCCGAAACCCTAACCCTAAGACCGAAATCGTTCGGAAATTACGCGATTAAAGCCCTAATCGTAGCGCTGATGGTGAAATCGTACGATTACTCTGAGTTTCTTCACCCGAAACTCGTTCTGTTCATTTATAGTTGGCATGTGTACTTCCTTCTCGAGATTATTTTGGCCATCACGCAGTTCCTCGGCCGGAATCTGCTCGGAATCGAGCTCGAGCCGCAGTTCAACGATCCGTATTTCTCGACTTCGCTTCAGGATTTTTGGGGGAGGCGGTGGAATCTGATGGCTACGAACATTCTTCAGCCGACGGTTTACAAGCCGTCGGTGAAGATCGCGGCTAAGGTCGTGGGGCGGAAGTGGGCGCCATTGCCGGCGGTGATGGCGACGTTTATGGTGTCGGCGATGATGCACGAACTGATATTTTACTATTTGGGTCGGATGAGGCCGAACTGGGAGATTACTTGGTTCTTTGTTATCCACGGGGTTGCTTTGACGGTGGAGATTCTGATGAAGAAGGCGTTGCCGGAGAAGTGGCGGGTGCCGGCGGTGGTGTCGGTGCCGGTGACTGTGGGTTTCATCTTCTGGACTGCGATTTGGTTGTTTTTTCCGCAGTTCGTGAGGTTGAAGATAGACGTGAGGGCCTTTGAGGAGTACGCTGCGTTGGTCACTTTTGTCAAGAAACTCGCTTTCTCATTGGTCAGTTGA

mRNA sequence

ATGGAGGGAGAGATTTTCAACTTCGTCAAGGTCTGGTTAGCAGTCTGTATCTCTCTCTGTTACTGCCACGCCGCCGGAAAACTCCTCCCTCCCGGCGCCGGCCGCTTCCTCGCCGTCGCCCCCGTCGTCGCCCTCTTCCTCCTCCTCCCTCTGCAACTCACCTCCATCCACCTCGGCGGCTCCACCTCCTTCTTCGTCGCTTGGCTCGCTAACTTCAAGCTCCTCCTCTTCGCCGCTGGCGACGGCCCTCTCTCCGCCGCCCCGCCGCTCTCTCTCCGCCGCTTCCTCGCACTCTCCTGCTTGCCGATCAAGATCCTCCAAAACCCCTCGGAGATATCGAGCCCTAACAATCGCGCCGAAACCCTAACCCTAAGACCGAAATCGTTCGGAAATTACGCGATTAAAGCCCTAATCGTAGCGCTGATGGTGAAATCGTACGATTACTCTGAGTTTCTTCACCCGAAACTCGTTCTGTTCATTTATAGTTGGCATGTGTACTTCCTTCTCGAGATTATTTTGGCCATCACGCAGTTCCTCGGCCGGAATCTGCTCGGAATCGAGCTCGAGCCGCAGTTCAACGATCCGTATTTCTCGACTTCGCTTCAGGATTTTTGGGGGAGGCGGTGGAATCTGATGGCTACGAACATTCTTCAGCCGACGGTTTACAAGCCGTCGGTGAAGATCGCGGCTAAGGTCGTGGGGCGGAAGTGGGCGCCATTGCCGGCGGTGATGGCGACGTTTATGGTGTCGGCGATGATGCACGAACTGATATTTTACTATTTGGGTCGGATGAGGCCGAACTGGGAGATTACTTGGTTCTTTGTTATCCACGGGGTTGCTTTGACGGTGGAGATTCTGATGAAGAAGGCGTTGCCGGAGAAGTGGCGGGTGCCGGCGGTGGTGTCGGTGCCGGTGACTGTGGGTTTCATCTTCTGGACTGCGATTTGGTTGTTTTTTCCGCAGTTCGTGAGGTTGAAGATAGACGTGAGGGCCTTTGAGGAGTACGCTGCGTTGGTCACTTTTGTCAAGAAACTCGCTTTCTCATTGGTCAGTTGA

Coding sequence (CDS)

ATGGAGGGAGAGATTTTCAACTTCGTCAAGGTCTGGTTAGCAGTCTGTATCTCTCTCTGTTACTGCCACGCCGCCGGAAAACTCCTCCCTCCCGGCGCCGGCCGCTTCCTCGCCGTCGCCCCCGTCGTCGCCCTCTTCCTCCTCCTCCCTCTGCAACTCACCTCCATCCACCTCGGCGGCTCCACCTCCTTCTTCGTCGCTTGGCTCGCTAACTTCAAGCTCCTCCTCTTCGCCGCTGGCGACGGCCCTCTCTCCGCCGCCCCGCCGCTCTCTCTCCGCCGCTTCCTCGCACTCTCCTGCTTGCCGATCAAGATCCTCCAAAACCCCTCGGAGATATCGAGCCCTAACAATCGCGCCGAAACCCTAACCCTAAGACCGAAATCGTTCGGAAATTACGCGATTAAAGCCCTAATCGTAGCGCTGATGGTGAAATCGTACGATTACTCTGAGTTTCTTCACCCGAAACTCGTTCTGTTCATTTATAGTTGGCATGTGTACTTCCTTCTCGAGATTATTTTGGCCATCACGCAGTTCCTCGGCCGGAATCTGCTCGGAATCGAGCTCGAGCCGCAGTTCAACGATCCGTATTTCTCGACTTCGCTTCAGGATTTTTGGGGGAGGCGGTGGAATCTGATGGCTACGAACATTCTTCAGCCGACGGTTTACAAGCCGTCGGTGAAGATCGCGGCTAAGGTCGTGGGGCGGAAGTGGGCGCCATTGCCGGCGGTGATGGCGACGTTTATGGTGTCGGCGATGATGCACGAACTGATATTTTACTATTTGGGTCGGATGAGGCCGAACTGGGAGATTACTTGGTTCTTTGTTATCCACGGGGTTGCTTTGACGGTGGAGATTCTGATGAAGAAGGCGTTGCCGGAGAAGTGGCGGGTGCCGGCGGTGGTGTCGGTGCCGGTGACTGTGGGTTTCATCTTCTGGACTGCGATTTGGTTGTTTTTTCCGCAGTTCGTGAGGTTGAAGATAGACGTGAGGGCCTTTGAGGAGTACGCTGCGTTGGTCACTTTTGTCAAGAAACTCGCTTTCTCATTGGTCAGTTGA

Protein sequence

MEGEIFNFVKVWLAVCISLCYCHAAGKLLPPGAGRFLAVAPVVALFLLLPLQLTSIHLGGSTSFFVAWLANFKLLLFAAGDGPLSAAPPLSLRRFLALSCLPIKILQNPSEISSPNNRAETLTLRPKSFGNYAIKALIVALMVKSYDYSEFLHPKLVLFIYSWHVYFLLEIILAITQFLGRNLLGIELEPQFNDPYFSTSLQDFWGRRWNLMATNILQPTVYKPSVKIAAKVVGRKWAPLPAVMATFMVSAMMHELIFYYLGRMRPNWEITWFFVIHGVALTVEILMKKALPEKWRVPAVVSVPVTVGFIFWTAIWLFFPQFVRLKIDVRAFEEYAALVTFVKKLAFSLVS

Homology

[Close]

BLAST of Lag0031126 vs. NCBI nr
Match: XP_022948970.1 (acyl-CoA--sterol O-acyltransferase 1-like [Cucurbita moschata] >KAG6606895.1 Acyl-CoA--sterol O-acyltransferase 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 607.8 bits (1566), Expect = 5.8e-170
Identity = 302/350 (86.29%), Postives = 321/350 (91.71%), Query Frame = 0


Query: 1   MEGEIFNFVKVWLAVCISLCYCHAAGKLLPPGAGRFLAVAPVVALFLLLPLQLTSIHLGG 60
           MEGEI NF+KVWL VCISLCYCHA GKLL PG GRFLAVAP+V LF LLPLQLTSIHLGG
Sbjct: 1   MEGEILNFIKVWLTVCISLCYCHATGKLLAPGPGRFLAVAPIVVLFFLLPLQLTSIHLGG 60

Query: 61  STSFFVAWLANFKLLLFAAGDGPLSAAPPLSLRRFLALSCLPIKILQNPSEISSPNNRAE 120
           STSFF+AWLANFKLLLFAAGDGPLSA PPL LRRFLALSCLPIKILQN  E S PNN   
Sbjct: 61  STSFFIAWLANFKLLLFAAGDGPLSAEPPLPLRRFLALSCLPIKILQN--EKSCPNNNRS 120

Query: 121 TLTLRPKSFGNYAIKALIVALMVKSYDYSEFLHPKLVLFIYSWHVYFLLEIILAITQFLG 180
             TL+PKSF NY+IKA+IVALMVK YDYSEF+HPK+VLFIYSWH+YFLLEIILAITQF G
Sbjct: 121 DTTLKPKSFLNYSIKAVIVALMVKLYDYSEFIHPKVVLFIYSWHLYFLLEIILAITQFFG 180

Query: 181 RNLLGIELEPQFNDPYFSTSLQDFWGRRWNLMATNILQPTVYKPSVKIAAKVVGRKWAPL 240
           RN+LGIELEPQF DPYFSTSLQDFWGRRWNLMATNILQPTVYKPSVKIAAKV+GRKWAPL
Sbjct: 181 RNVLGIELEPQFQDPYFSTSLQDFWGRRWNLMATNILQPTVYKPSVKIAAKVIGRKWAPL 240

Query: 241 PAVMATFMVSAMMHELIFYYLGRMRPNWEITWFFVIHGVALTVEILMKKALPEKWRVPAV 300
           PAVMATF+VSAMMHELIFYY+GRMRPNWE TWFFVIHGVALTVEI++KK LPEKWR+P  
Sbjct: 241 PAVMATFLVSAMMHELIFYYMGRMRPNWECTWFFVIHGVALTVEIMVKKVLPEKWRLPPA 300

Query: 301 VSVPVTVGFIFWTAIWLFFPQFVRLKIDVRAFEEYAALVTFVKKLAFSLV 351
           VSVPVT+ FIF TAIWLFFPQFVRLK+DVRAFEEYAALVTFVK +AFSLV
Sbjct: 301 VSVPVTLAFIFPTAIWLFFPQFVRLKLDVRAFEEYAALVTFVKNVAFSLV 348

[Close]

BLAST of Lag0031126 vs. NCBI nr
Match: XP_022998466.1 (acyl-CoA--sterol O-acyltransferase 1-like [Cucurbita maxima])

HSP 1 Score: 607.4 bits (1565), Expect = 7.5e-170
Identity = 300/350 (85.71%), Postives = 321/350 (91.71%), Query Frame = 0


Query: 1   MEGEIFNFVKVWLAVCISLCYCHAAGKLLPPGAGRFLAVAPVVALFLLLPLQLTSIHLGG 60
           MEGEI NF+KVWL VCISLCYCHA GKL+PPGAGRFLAVAP+V LF  LPLQLTSIHLGG
Sbjct: 1   MEGEILNFIKVWLTVCISLCYCHATGKLIPPGAGRFLAVAPIVVLFFFLPLQLTSIHLGG 60

Query: 61  STSFFVAWLANFKLLLFAAGDGPLSAAPPLSLRRFLALSCLPIKILQNPSEISSPNNRAE 120
           STSFF+AWLANFKLLLF AGDGPLSA PPL LRRFLALSCLPIKILQN  E S PNN   
Sbjct: 61  STSFFIAWLANFKLLLFTAGDGPLSAEPPLPLRRFLALSCLPIKILQN--EKSCPNNNRS 120

Query: 121 TLTLRPKSFGNYAIKALIVALMVKSYDYSEFLHPKLVLFIYSWHVYFLLEIILAITQFLG 180
             TL+PKSF NY+IKA+IVALMVK YDYSEF+HPK+VLFIYSWH+YFLLEIILAITQF G
Sbjct: 121 DTTLKPKSFLNYSIKAVIVALMVKLYDYSEFIHPKVVLFIYSWHLYFLLEIILAITQFFG 180

Query: 181 RNLLGIELEPQFNDPYFSTSLQDFWGRRWNLMATNILQPTVYKPSVKIAAKVVGRKWAPL 240
           RN+LGIELEPQF DPYFSTSLQDFWGRRWNLMATNILQPTVYKPSVKIAAKV+GRKWAPL
Sbjct: 181 RNVLGIELEPQFQDPYFSTSLQDFWGRRWNLMATNILQPTVYKPSVKIAAKVIGRKWAPL 240

Query: 241 PAVMATFMVSAMMHELIFYYLGRMRPNWEITWFFVIHGVALTVEILMKKALPEKWRVPAV 300
           PAVMATF+VSAMMHELIF+Y+GRMRPNWE TWFFVIHGVALTVEI++KK LPEKWR+P  
Sbjct: 241 PAVMATFLVSAMMHELIFFYMGRMRPNWECTWFFVIHGVALTVEIMVKKVLPEKWRLPPA 300

Query: 301 VSVPVTVGFIFWTAIWLFFPQFVRLKIDVRAFEEYAALVTFVKKLAFSLV 351
           VSVPVT+ FIF TAIWLFFPQFVRLK+DVRAFEEYAALVTFVK +AFSLV
Sbjct: 301 VSVPVTLAFIFPTAIWLFFPQFVRLKLDVRAFEEYAALVTFVKNVAFSLV 348

[Close]

BLAST of Lag0031126 vs. NCBI nr
Match: XP_022934183.1 (acyl-CoA--sterol O-acyltransferase 1-like [Cucurbita moschata])

HSP 1 Score: 599.7 bits (1545), Expect = 1.6e-167
Identity = 294/351 (83.76%), Postives = 320/351 (91.17%), Query Frame = 0


Query: 1   MEGEIFNFVKVWLAVCISLCYCHAAGKLLPPGAGRFLAVAPVVALFLLLPLQLTSIHLGG 60
           MEGE+ NF+ VWL VC+SLCYCH AGK+LPPGAGRFLA+APVV LF LLPLQLTS+HLGG
Sbjct: 1   MEGEVINFIMVWLIVCMSLCYCHTAGKILPPGAGRFLAIAPVVVLFFLLPLQLTSVHLGG 60

Query: 61  STSFFVAWLANFKLLLFAAGDGPLSAAPPLSLRRFLALSCLPIKILQNPSEISSPNNRAE 120
           ST+FF+AWLANFKLLLF AG+GPLSA PPL LRRFLALSCLPIKILQNPSE  SP N  E
Sbjct: 61  STAFFIAWLANFKLLLFTAGNGPLSATPPLPLRRFLALSCLPIKILQNPSEKQSPKNHHE 120

Query: 121 TLTLRPKSFGNYAIKALIVALMVKSYDYSEFLHPKLVLFIYSWHVYFLLEIILAITQFLG 180
           TL   PKSF NY IKALIVALMVK YDYSEF+HPK++LFIYSWHVYF LEIILAITQF G
Sbjct: 121 TLNQEPKSFLNYWIKALIVALMVKLYDYSEFIHPKVILFIYSWHVYFFLEIILAITQFFG 180

Query: 181 RNLLGIELEPQFNDPYFSTSLQDFWGRRWNLMATNILQPTVYKPSVKIAAKVVGRKWAPL 240
           R LLGIELEPQF+DPYFSTSLQDFWGRRWNLMATNILQPT+YKP+VKIAAKVVGRKWAPL
Sbjct: 181 RTLLGIELEPQFHDPYFSTSLQDFWGRRWNLMATNILQPTIYKPTVKIAAKVVGRKWAPL 240

Query: 241 PAVMATFMVSAMMHELIFYYLGRMRPNWEITWFFVIHGVALTVEILMKKALPEKWRVPAV 300
           P VMATF+VSA+MHELIFYYLGRM+PNWE+TWFFVIHG ALTVEIL+KK LPEK R+PA 
Sbjct: 241 PGVMATFLVSAIMHELIFYYLGRMKPNWELTWFFVIHGFALTVEILVKKVLPEKRRLPAA 300

Query: 301 VSVPVTVGFIFWTAIWLFFPQFVRLKIDVRAFEEYAALVTFVKKLAFSLVS 352
           VSVPVT+ FIF TAIWLFFPQF+RLKIDV+AFEEYAALV+F+KKLAF LVS
Sbjct: 301 VSVPVTLVFIFSTAIWLFFPQFMRLKIDVKAFEEYAALVSFIKKLAFPLVS 351

[Close]

BLAST of Lag0031126 vs. NCBI nr
Match: XP_023527814.1 (acyl-CoA--sterol O-acyltransferase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 599.0 bits (1543), Expect = 2.7e-167
Identity = 295/351 (84.05%), Postives = 320/351 (91.17%), Query Frame = 0


Query: 1   MEGEIFNFVKVWLAVCISLCYCHAAGKLLPPGAGRFLAVAPVVALFLLLPLQLTSIHLGG 60
           MEGEI NF  VWL VC+SLCYCH AGK+LPPGAGRFLA+APVV LF LLPLQLTS+HLGG
Sbjct: 1   MEGEIINFTMVWLIVCMSLCYCHTAGKILPPGAGRFLAIAPVVLLFFLLPLQLTSVHLGG 60

Query: 61  STSFFVAWLANFKLLLFAAGDGPLSAAPPLSLRRFLALSCLPIKILQNPSEISSPNNRAE 120
           +T+FF+AWLANFKLLLFAAG+GPLSA PPL LRRFLALSCLPIKILQNPSE  SP N  E
Sbjct: 61  ATAFFIAWLANFKLLLFAAGNGPLSATPPLPLRRFLALSCLPIKILQNPSEKQSPKNHHE 120

Query: 121 TLTLRPKSFGNYAIKALIVALMVKSYDYSEFLHPKLVLFIYSWHVYFLLEIILAITQFLG 180
           TL   PKSF NY IKALIVALMVK YDYSEF+HPK++LFIYSWHVYF LEIILAITQF G
Sbjct: 121 TLNQEPKSFLNYWIKALIVALMVKLYDYSEFIHPKVILFIYSWHVYFFLEIILAITQFFG 180

Query: 181 RNLLGIELEPQFNDPYFSTSLQDFWGRRWNLMATNILQPTVYKPSVKIAAKVVGRKWAPL 240
           R LLGIELEPQF+DPYFSTSLQDFWGRRWNLMATNILQPT+YKP+VKIAAKVVGRKWAPL
Sbjct: 181 RTLLGIELEPQFHDPYFSTSLQDFWGRRWNLMATNILQPTIYKPTVKIAAKVVGRKWAPL 240

Query: 241 PAVMATFMVSAMMHELIFYYLGRMRPNWEITWFFVIHGVALTVEILMKKALPEKWRVPAV 300
           P VMATF+VSA+MHELIFYYLGRM+PNWE+TWFFVIHG ALTVEIL+KK LPEK R+PA 
Sbjct: 241 PGVMATFLVSAIMHELIFYYLGRMKPNWELTWFFVIHGFALTVEILVKKVLPEKRRLPAA 300

Query: 301 VSVPVTVGFIFWTAIWLFFPQFVRLKIDVRAFEEYAALVTFVKKLAFSLVS 352
           VSVPVT+ FIF TAIWLFFPQF+RLKIDV+AFEEYAALV+F+KKLAF LVS
Sbjct: 301 VSVPVTLVFIFSTAIWLFFPQFMRLKIDVKAFEEYAALVSFIKKLAFPLVS 351

[Close]

BLAST of Lag0031126 vs. NCBI nr
Match: XP_023523550.1 (LOW QUALITY PROTEIN: acyl-CoA--sterol O-acyltransferase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 595.9 bits (1535), Expect = 2.3e-166
Identity = 296/347 (85.30%), Postives = 316/347 (91.07%), Query Frame = 0


Query: 1   MEGEIFNFVKVWLAVCISLCYCHAAGKLLPPGAGRFLAVAPVVALFLLLPLQLTSIHLGG 60
           MEGEI NF+KVWL VCISLCYCHA GKLLPPGAGRFLAVAP+V LF LLPLQLTSIHLGG
Sbjct: 1   MEGEILNFIKVWLTVCISLCYCHATGKLLPPGAGRFLAVAPIVVLFFLLPLQLTSIHLGG 60

Query: 61  STSFFVAWLANFKLLLFAAGDGPLSAAPPLSLRRFLALSCLPIKILQNPSEISSPNNRAE 120
           STSFF+AWLANFKLLLFAAGDGPLSA  PL LRRFLALSCLPIKILQN  E S PNN   
Sbjct: 61  STSFFIAWLANFKLLLFAAGDGPLSAEQPLPLRRFLALSCLPIKILQN--EKSCPNNNRS 120

Query: 121 TLTLRPKSFGNYAIKALIVALMVKSYDYSEFLHPKLVLFIYSWHVYFLLEIILAITQFLG 180
             TL+PKSF NY+IKA+IVALMVK YDYSEF+HPK+VLFIYSWH+YFLLEIILAITQF G
Sbjct: 121 DTTLKPKSFLNYSIKAVIVALMVKLYDYSEFIHPKVVLFIYSWHLYFLLEIILAITQFFG 180

Query: 181 RNLLGIELEPQFNDPYFSTSLQDFWGRRWNLMATNILQPTVYKPSVKIAAKVVGRKWAPL 240
           RN+LGIELEPQF DPYFSTSLQDFWGRRWNLMATNILQPTVYKPSVKIAAKV+GRKWAPL
Sbjct: 181 RNVLGIELEPQFQDPYFSTSLQDFWGRRWNLMATNILQPTVYKPSVKIAAKVIGRKWAPL 240

Query: 241 PAVMATFMVSAMMHELIFYYLGRMRPNWEITWFFVIHGVALTVEILMKKALPEKWRVPAV 300
           PAVMATF+VSAMMHELIFYY+GRMRPNWE TWFFVIHGVALTVEI++KKALPEKWR+P  
Sbjct: 241 PAVMATFIVSAMMHELIFYYMGRMRPNWECTWFFVIHGVALTVEIMVKKALPEKWRLPPA 300

Query: 301 VSVPVTVGFIFWTAIWLFFPQFVRLKIDVRAFEEYAALVTFVKKLAF 348
           VSVPVT+ FIF TAIWLFFPQFVRL++DVRAFEEYAAL  F K+  F
Sbjct: 301 VSVPVTLAFIFPTAIWLFFPQFVRLQLDVRAFEEYAALXHFCKERRF 345

[Close]

BLAST of Lag0031126 vs. ExPASy Swiss-Prot
Match: Q9SV07 (Acyl-CoA--sterol O-acyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=ASAT1 PE=1 SV=1)

HSP 1 Score: 317.4 bits (812), Expect = 2.0e-85
Identity = 168/346 (48.55%), Postives = 233/346 (67.34%), Query Frame = 0


Query: 7   NFVKVWLAVCISLCYCHAAGKLLPPGAGRFLAVAPVVALFLLLPLQLTSIHLGGSTSFFV 66
           +F+K W  V ISLCY     KL+P G  R +   PV  +F ++P  + S+HL G T+FF+
Sbjct: 3   SFIKAWGLVIISLCYTFFIAKLVPKGIKRLILFFPVFLIFFIVPFLIYSLHLLGITAFFI 62

Query: 67  AWLANFKLLLFAAGDGPLSA-APPLSLRRFLALSCLPIKILQNPSEISSPNNRAETLTLR 126
           AWLANFKLLLFA G GPLS+   PLSL  FLA+SCLPIKI  +P    + ++   T    
Sbjct: 63  AWLANFKLLLFALGRGPLSSNHKPLSLPIFLAVSCLPIKIQLSPKPTKTHSHEGST---- 122

Query: 127 PKSFGNYAIKALIVALMVKSYDYSEFLHPKLVLFIYSWHVYFLLEIILAITQFLGRNLLG 186
            +    Y IKA+ V L++K+Y+YS  L  K+VL +Y+ H+YF LEIILA T    R +  
Sbjct: 123 -EGPLIYTIKAVFVVLIIKAYEYSTKLPEKVVLTLYAIHIYFALEIILAATAAAVRAMSD 182

Query: 187 IELEPQFNDPYFSTSLQDFWGRRWNLMATNILQPTVYKPSVKIAAKVVGRKWAPLPAVMA 246
           +ELEPQFN PY +TSLQDFWGRRWNLM T IL+PTVY+PS+++ + V+G  ++ + A   
Sbjct: 183 LELEPQFNKPYLATSLQDFWGRRWNLMVTGILRPTVYEPSLQLFS-VLGPNYSQILAAFG 242

Query: 247 TFMVSAMMHELIFYYLGRMRPNWEITWFFVIHGVALTVEILMKKALPEKWRVPAVVSVPV 306
           TF+VS +MHELIF+Y+GR+RP+W++ WFF+I+G   TVEI +KK +  +WR P  +S  +
Sbjct: 243 TFVVSGIMHELIFFYMGRLRPDWKMMWFFLINGFCTTVEIAIKKTINGRWRFPKAISQVL 302

Query: 307 TVGFIFWTAIWLFFPQFVRLKIDVRAFEEYAALVTFVKKLAFSLVS 352
           T+ F+  TA+WLF P+F R  I  +A +EYAA+  F  ++   L +
Sbjct: 303 TLTFVMVTALWLFLPEFNRCNIVEKALDEYAAIGAFAVEVRRKLTA 342

[Close]

BLAST of Lag0031126 vs. ExPASy Swiss-Prot
Match: Q9XGY6 (Long-chain-alcohol O-fatty-acyltransferase OS=Simmondsia chinensis OX=3999 PE=1 SV=1)

HSP 1 Score: 273.5 bits (698), Expect = 3.4e-72
Identity = 149/351 (42.45%), Postives = 217/351 (61.82%), Query Frame = 0


Query: 1   MEGEIFNFVKVWLAVCISLCYCHAAGKLLP-PGAGRFLAVAPVVALFLLLPLQLTSIHLG 60
           +E E+  F +VW++   + CYC     + P  GA R L + PVV LF+ LPL+L+S HLG
Sbjct: 3   VEKELKTFSEVWISAIAAACYCRFVPAVAPHGGALRLLLLLPVVLLFIFLPLRLSSFHLG 62

Query: 61  GSTSFFVAWLANFKLLLFAAGDGPLSAAPPLSLRRFLALSCLPIKILQNPSEISSPNNRA 120
           G T+ ++ WLANFKLLLFA   GPLS  P LSL  F++ + LPIK   +PS     N R 
Sbjct: 63  GPTALYLVWLANFKLLLFAFHLGPLS-NPSLSLLHFISTTLLPIKFRDDPSNDHEKNKR- 122

Query: 121 ETLTLRPKSFGNYAIKALIVALMVKSYDYSEFLHPKLVLFIYSWHVYFLLEIILAITQFL 180
            TL+   +    +  K +  A ++K Y++ + L   ++  +Y +H Y   EI LA +  +
Sbjct: 123 -TLSFEWRKVVLFVAKLVFFAGILKIYEFRKDLPHFVISVLYCFHFYLGTEITLAASAVI 182

Query: 181 GRNLLGIELEPQFNDPYFSTSLQDFWGRRWNLMATNILQPTVYKPSVKIAAKVVGRKWAP 240
            R  LG++L PQFN+PY +TSLQDFWGRRWNLM ++IL  T Y+P  ++ ++ V  +W  
Sbjct: 183 ARATLGLDLYPQFNEPYLATSLQDFWGRRWNLMVSDILGLTTYQPVRRVLSRWVRLRWEV 242

Query: 241 LPAVMATFMVSAMMHELIFYYLGRMRPNWEITWFFVIHGVALTVEILMKKALPEKWRVPA 300
             A++  F VS +MHE+ F+YL R RP+WE+T FFV+HGV   VE+++KKA+  K R+  
Sbjct: 243 AGAMLVAFTVSGLMHEVFFFYLTRARPSWEVTGFFVLHGVCTAVEMVVKKAVSGKVRLRR 302

Query: 301 VVSVPVTVGFIFWTAIWLFFPQFVRLKIDVRAFEEYAALVTFVKKLAFSLV 351
            VS  +TVGF+  T  WLF PQ VR  +D++  +EY  +  + +K    L+
Sbjct: 303 EVSGALTVGFVMVTGGWLFLPQLVRHGVDLKTIDEYPVMFNYTQKKLMGLL 350

[Close]

BLAST of Lag0031126 vs. ExPASy Swiss-Prot
Match: Q9FJ76 (Probable long-chain-alcohol O-fatty-acyltransferase 5 OS=Arabidopsis thaliana OX=3702 GN=AT5 PE=2 SV=1)

HSP 1 Score: 272.3 bits (695), Expect = 7.5e-72
Identity = 150/346 (43.35%), Postives = 214/346 (61.85%), Query Frame = 0


Query: 1   MEGEIFNFVKVWLAVCISLCYCHAAGKLLPPGAGRFLAVAPVVALFLLLPLQLTSIHLGG 60
           M+ E+ N +KVW++  IS+ YC+     +  GA RFL+V+PV+ALFL+LPL  +S+HL  
Sbjct: 1   MDEELKNLIKVWVSAIISISYCYYIPPRIKSGAPRFLSVSPVLALFLVLPLFFSSLHLSL 60

Query: 61  STSFFVAWLANFKLLLFAAGDGPLSAAPPLSLRRFLALSCLPIKILQNPSEISSPNNRAE 120
            T+FF+ WLANFKL+LF+   GPL    P +  RF   +C PIK+ QNP    S N+  +
Sbjct: 61  ITAFFLTWLANFKLILFSFDKGPLIPI-PTNFPRFFCFTCFPIKVQQNP---KSQNHLPK 120

Query: 121 TLTLRPKSFGNYAIKALIVALMVKSYDYSEFLHPKLVLFIYSWHVYFLLEIILAITQFLG 180
            +         +AIK  I A+++  Y Y + L P ++L +Y  H+Y  +EIIL   + + 
Sbjct: 121 LV---------FAIKLAIFAVLLHLYSYRQNLSPTILLGLYFVHLYLEIEIILTFVKVVV 180

Query: 181 RNLLGIELEPQFNDPYFSTSLQDFWGRRWNLMATNILQPTVYKPSVKIAAKVVGRKWAPL 240
              LG +LEPQ N PY +TSLQDFWGRRWNLM   IL+P VY P  +++ + +   WA  
Sbjct: 181 FISLGCDLEPQSNKPYLATSLQDFWGRRWNLMVPAILRPAVYAPMRRVSERKMSSGWALF 240

Query: 241 PAVMATFMVSAMMHELIFYYLGRMRPNWEITWFFVIHGVALTVEILMKK--ALPEKWRVP 300
           P ++A F+VS ++HEL+F+YL R  P  E+T FFV+HGV   VE+ +KK   + ++WR+ 
Sbjct: 241 PGILAAFIVSGLVHELLFFYLIREMPTGEVTLFFVLHGVCTAVELAVKKKTTVAQRWRLS 300

Query: 301 AVVSVPVTVGFIFWTAIWLFFPQFVRLKIDVRAFEEYAALVTFVKK 345
             VS  +TVGF+F T  WLF PQ  R  +  R   E   LV F+K+
Sbjct: 301 PGVSRVLTVGFVFVTGGWLFTPQLKRSGVMERFTSEAVLLVEFIKR 333

[Close]

BLAST of Lag0031126 vs. ExPASy Swiss-Prot
Match: Q9FJ75 (Probable long-chain-alcohol O-fatty-acyltransferase 4 OS=Arabidopsis thaliana OX=3702 GN=AT4 PE=2 SV=1)

HSP 1 Score: 263.1 bits (671), Expect = 4.6e-69
Identity = 148/350 (42.29%), Postives = 207/350 (59.14%), Query Frame = 0


Query: 1   MEGEIFNFVKVWLAVCISLCYCHAAGKLLPPGAGRFLAVAPVVALFLLLPLQLTSIHLGG 60
           ME E+ + +KVW+   IS+ YC+     +  G  R L+V PV  LFL+LPL ++S+H  G
Sbjct: 1   MEEELMSLIKVWVYAIISISYCYYTSTRIKSGVFRLLSVLPVCVLFLVLPLFVSSVHFSG 60

Query: 61  STSFFVAWLANFKLLLFAAGDGPLSAAPPLSLRRFLALSCLPIKILQNPSEISSPNNRAE 120
           ST+FF++WLANFKL+LF+   GPL   P  +L RF+  +C PIK+ QNP     P N+  
Sbjct: 61  STAFFLSWLANFKLILFSFDQGPLFPVPS-NLSRFVCFTCFPIKLQQNP----KPQNQ-- 120

Query: 121 TLTLRPKSFGNYAIKALIVALMVKSYDYSEFLHPKLVLFIYSWHVYFLLEIILAITQFLG 180
                PK    +A+K     +++  Y+Y + + P ++L +YS H+Y   EI+LA  + L 
Sbjct: 121 ----MPK--WGFAVKLAFFGVLLHMYEYKQHMSPTVLLVLYSLHIYLEYEILLAPLKVLL 180

Query: 181 RNLLGIELEPQFNDPYFSTSLQDFWGRRWNLMATNILQPTVYKPSVKIAAKVVGRKWAPL 240
              L  +LEP FN+PY STSLQDFWGRRWNLM   IL+P VY P  ++A + +    A  
Sbjct: 181 SISLWCDLEPHFNEPYLSTSLQDFWGRRWNLMVPAILRPAVYLPVRQMAGRKMNSDQALF 240

Query: 241 PAVMATFMVSAMMHELIFYYLGRMRPNWEITWFFVIHGVALTVEILMKKA-LPEKWRVPA 300
             V A+F+VS ++HELIF+Y  R  P  E+T FFV+HGV    E   K+  L  +W+V  
Sbjct: 241 LGVFASFLVSGVVHELIFFYFTRESPTGEVTLFFVLHGVCTAAECAAKRTRLVRRWKVSQ 300

Query: 301 VVSVPVTVGFIFWTAIWLFFPQFVRLKIDVRAFEEYAALVTFVKKLAFSL 350
           +VS  +TVGF+  T  WLFFP   R  +  R  +E    + FVK   F L
Sbjct: 301 MVSRLLTVGFVVMTGGWLFFPHLARSGMIERLADEAFLFIGFVKHKFFYL 337

[Close]

BLAST of Lag0031126 vs. ExPASy Swiss-Prot
Match: Q9FJ74 (Probable long-chain-alcohol O-fatty-acyltransferase 3 OS=Arabidopsis thaliana OX=3702 GN=AT3 PE=3 SV=1)

HSP 1 Score: 258.8 bits (660), Expect = 8.6e-68
Identity = 145/345 (42.03%), Postives = 208/345 (60.29%), Query Frame = 0


Query: 1   MEGEIFNFVKVWLAVCISLCYCHAAGKLLPPGAGRFLAVAPVVALFLLLPLQLTSIHLGG 60
           ME E+ NF+K+W++  IS+ YC+     +  G  R L+V PV ALFL+ PL  + +H  G
Sbjct: 1   MEEELKNFIKLWISAIISISYCYYLSTGIKAGVFRLLSVLPVCALFLVFPLFFSYVHFSG 60

Query: 61  STSFFVAWLANFKLLLFAAGDGPLSAAPPLSLRRFLALSCLPIKILQNPSEISSPNNRAE 120
             +FF++WLANFKL+LF+   GPLS   P +L RF+ ++C PIK  QNP    +  N   
Sbjct: 61  CMAFFLSWLANFKLILFSFDQGPLSPL-PRTLSRFICITCFPIKPQQNP----NIQNYKI 120

Query: 121 TLTLRPKSFGNYAIKALIVALMVKSYDYSEFLHPKLVLFIYSWHVYFLLEIILAITQFLG 180
            + L       +AIK +I  ++++ Y+Y ++L P L+L   S H++  LEI+  + + L 
Sbjct: 121 PIWL-------FAIKVVIFVVLLQMYEYKQYLSPALLLVFNSLHIFLELEIVFMLVKALV 180

Query: 181 RNLLGIELEPQFNDPYFSTSLQDFWGRRWNLMATNILQPTVYKPSVKIAAKVVGRKWAPL 240
              LG +LEPQ N+PY +TSLQDFWGRRWNLM   IL+P VY P+ ++A + V    A  
Sbjct: 181 FITLGCDLEPQSNEPYLATSLQDFWGRRWNLMVPAILRPAVYLPARRMACRKVNSDQAMF 240

Query: 241 PAVMATFMVSAMMHELIFYYLGRMRPNWEITWFFVIHGVALTVEILMKKA--LPEKWRVP 300
             V A F+VS  +HE++F+YL R  P  E+TWFF++HGV    E+ +KK+  +   WRV 
Sbjct: 241 LGVFAAFLVSGAVHEMLFFYLTREVPTGEVTWFFLLHGVCTVAEVAVKKSTFVRRWWRVS 300

Query: 301 AVVSVPVTVGFIFWTAIWLFFPQFVRLKIDVRAFEEYAALVTFVK 344
             VS  +TVGF+  T+ W FFP  +R  I  R   E    + FVK
Sbjct: 301 PTVSRLLTVGFVVVTSGWFFFP-LIRSGIIERLASEALMCIDFVK 332

[Close]

BLAST of Lag0031126 vs. ExPASy TrEMBL
Match: A0A6J1GBE5 (acyl-CoA--sterol O-acyltransferase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111452458 PE=3 SV=1)

HSP 1 Score: 607.8 bits (1566), Expect = 2.8e-170
Identity = 302/350 (86.29%), Postives = 321/350 (91.71%), Query Frame = 0


Query: 1   MEGEIFNFVKVWLAVCISLCYCHAAGKLLPPGAGRFLAVAPVVALFLLLPLQLTSIHLGG 60
           MEGEI NF+KVWL VCISLCYCHA GKLL PG GRFLAVAP+V LF LLPLQLTSIHLGG
Sbjct: 1   MEGEILNFIKVWLTVCISLCYCHATGKLLAPGPGRFLAVAPIVVLFFLLPLQLTSIHLGG 60

Query: 61  STSFFVAWLANFKLLLFAAGDGPLSAAPPLSLRRFLALSCLPIKILQNPSEISSPNNRAE 120
           STSFF+AWLANFKLLLFAAGDGPLSA PPL LRRFLALSCLPIKILQN  E S PNN   
Sbjct: 61  STSFFIAWLANFKLLLFAAGDGPLSAEPPLPLRRFLALSCLPIKILQN--EKSCPNNNRS 120

Query: 121 TLTLRPKSFGNYAIKALIVALMVKSYDYSEFLHPKLVLFIYSWHVYFLLEIILAITQFLG 180
             TL+PKSF NY+IKA+IVALMVK YDYSEF+HPK+VLFIYSWH+YFLLEIILAITQF G
Sbjct: 121 DTTLKPKSFLNYSIKAVIVALMVKLYDYSEFIHPKVVLFIYSWHLYFLLEIILAITQFFG 180

Query: 181 RNLLGIELEPQFNDPYFSTSLQDFWGRRWNLMATNILQPTVYKPSVKIAAKVVGRKWAPL 240
           RN+LGIELEPQF DPYFSTSLQDFWGRRWNLMATNILQPTVYKPSVKIAAKV+GRKWAPL
Sbjct: 181 RNVLGIELEPQFQDPYFSTSLQDFWGRRWNLMATNILQPTVYKPSVKIAAKVIGRKWAPL 240

Query: 241 PAVMATFMVSAMMHELIFYYLGRMRPNWEITWFFVIHGVALTVEILMKKALPEKWRVPAV 300
           PAVMATF+VSAMMHELIFYY+GRMRPNWE TWFFVIHGVALTVEI++KK LPEKWR+P  
Sbjct: 241 PAVMATFLVSAMMHELIFYYMGRMRPNWECTWFFVIHGVALTVEIMVKKVLPEKWRLPPA 300

Query: 301 VSVPVTVGFIFWTAIWLFFPQFVRLKIDVRAFEEYAALVTFVKKLAFSLV 351
           VSVPVT+ FIF TAIWLFFPQFVRLK+DVRAFEEYAALVTFVK +AFSLV
Sbjct: 301 VSVPVTLAFIFPTAIWLFFPQFVRLKLDVRAFEEYAALVTFVKNVAFSLV 348

[Close]

BLAST of Lag0031126 vs. ExPASy TrEMBL
Match: A0A6J1KAA2 (acyl-CoA--sterol O-acyltransferase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111493091 PE=3 SV=1)

HSP 1 Score: 607.4 bits (1565), Expect = 3.7e-170
Identity = 300/350 (85.71%), Postives = 321/350 (91.71%), Query Frame = 0


Query: 1   MEGEIFNFVKVWLAVCISLCYCHAAGKLLPPGAGRFLAVAPVVALFLLLPLQLTSIHLGG 60
           MEGEI NF+KVWL VCISLCYCHA GKL+PPGAGRFLAVAP+V LF  LPLQLTSIHLGG
Sbjct: 1   MEGEILNFIKVWLTVCISLCYCHATGKLIPPGAGRFLAVAPIVVLFFFLPLQLTSIHLGG 60

Query: 61  STSFFVAWLANFKLLLFAAGDGPLSAAPPLSLRRFLALSCLPIKILQNPSEISSPNNRAE 120
           STSFF+AWLANFKLLLF AGDGPLSA PPL LRRFLALSCLPIKILQN  E S PNN   
Sbjct: 61  STSFFIAWLANFKLLLFTAGDGPLSAEPPLPLRRFLALSCLPIKILQN--EKSCPNNNRS 120

Query: 121 TLTLRPKSFGNYAIKALIVALMVKSYDYSEFLHPKLVLFIYSWHVYFLLEIILAITQFLG 180
             TL+PKSF NY+IKA+IVALMVK YDYSEF+HPK+VLFIYSWH+YFLLEIILAITQF G
Sbjct: 121 DTTLKPKSFLNYSIKAVIVALMVKLYDYSEFIHPKVVLFIYSWHLYFLLEIILAITQFFG 180

Query: 181 RNLLGIELEPQFNDPYFSTSLQDFWGRRWNLMATNILQPTVYKPSVKIAAKVVGRKWAPL 240
           RN+LGIELEPQF DPYFSTSLQDFWGRRWNLMATNILQPTVYKPSVKIAAKV+GRKWAPL
Sbjct: 181 RNVLGIELEPQFQDPYFSTSLQDFWGRRWNLMATNILQPTVYKPSVKIAAKVIGRKWAPL 240

Query: 241 PAVMATFMVSAMMHELIFYYLGRMRPNWEITWFFVIHGVALTVEILMKKALPEKWRVPAV 300
           PAVMATF+VSAMMHELIF+Y+GRMRPNWE TWFFVIHGVALTVEI++KK LPEKWR+P  
Sbjct: 241 PAVMATFLVSAMMHELIFFYMGRMRPNWECTWFFVIHGVALTVEIMVKKVLPEKWRLPPA 300

Query: 301 VSVPVTVGFIFWTAIWLFFPQFVRLKIDVRAFEEYAALVTFVKKLAFSLV 351
           VSVPVT+ FIF TAIWLFFPQFVRLK+DVRAFEEYAALVTFVK +AFSLV
Sbjct: 301 VSVPVTLAFIFPTAIWLFFPQFVRLKLDVRAFEEYAALVTFVKNVAFSLV 348

[Close]

BLAST of Lag0031126 vs. ExPASy TrEMBL
Match: A0A6J1F6Y6 (acyl-CoA--sterol O-acyltransferase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111441427 PE=3 SV=1)

HSP 1 Score: 599.7 bits (1545), Expect = 7.6e-168
Identity = 294/351 (83.76%), Postives = 320/351 (91.17%), Query Frame = 0


Query: 1   MEGEIFNFVKVWLAVCISLCYCHAAGKLLPPGAGRFLAVAPVVALFLLLPLQLTSIHLGG 60
           MEGE+ NF+ VWL VC+SLCYCH AGK+LPPGAGRFLA+APVV LF LLPLQLTS+HLGG
Sbjct: 1   MEGEVINFIMVWLIVCMSLCYCHTAGKILPPGAGRFLAIAPVVVLFFLLPLQLTSVHLGG 60

Query: 61  STSFFVAWLANFKLLLFAAGDGPLSAAPPLSLRRFLALSCLPIKILQNPSEISSPNNRAE 120
           ST+FF+AWLANFKLLLF AG+GPLSA PPL LRRFLALSCLPIKILQNPSE  SP N  E
Sbjct: 61  STAFFIAWLANFKLLLFTAGNGPLSATPPLPLRRFLALSCLPIKILQNPSEKQSPKNHHE 120

Query: 121 TLTLRPKSFGNYAIKALIVALMVKSYDYSEFLHPKLVLFIYSWHVYFLLEIILAITQFLG 180
           TL   PKSF NY IKALIVALMVK YDYSEF+HPK++LFIYSWHVYF LEIILAITQF G
Sbjct: 121 TLNQEPKSFLNYWIKALIVALMVKLYDYSEFIHPKVILFIYSWHVYFFLEIILAITQFFG 180

Query: 181 RNLLGIELEPQFNDPYFSTSLQDFWGRRWNLMATNILQPTVYKPSVKIAAKVVGRKWAPL 240
           R LLGIELEPQF+DPYFSTSLQDFWGRRWNLMATNILQPT+YKP+VKIAAKVVGRKWAPL
Sbjct: 181 RTLLGIELEPQFHDPYFSTSLQDFWGRRWNLMATNILQPTIYKPTVKIAAKVVGRKWAPL 240

Query: 241 PAVMATFMVSAMMHELIFYYLGRMRPNWEITWFFVIHGVALTVEILMKKALPEKWRVPAV 300
           P VMATF+VSA+MHELIFYYLGRM+PNWE+TWFFVIHG ALTVEIL+KK LPEK R+PA 
Sbjct: 241 PGVMATFLVSAIMHELIFYYLGRMKPNWELTWFFVIHGFALTVEILVKKVLPEKRRLPAA 300

Query: 301 VSVPVTVGFIFWTAIWLFFPQFVRLKIDVRAFEEYAALVTFVKKLAFSLVS 352
           VSVPVT+ FIF TAIWLFFPQF+RLKIDV+AFEEYAALV+F+KKLAF LVS
Sbjct: 301 VSVPVTLVFIFSTAIWLFFPQFMRLKIDVKAFEEYAALVSFIKKLAFPLVS 351

[Close]

BLAST of Lag0031126 vs. ExPASy TrEMBL
Match: A0A6J1J5C4 (acyl-CoA--sterol O-acyltransferase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111481453 PE=3 SV=1)

HSP 1 Score: 594.0 bits (1530), Expect = 4.2e-166
Identity = 294/351 (83.76%), Postives = 319/351 (90.88%), Query Frame = 0


Query: 1   MEGEIFNFVKVWLAVCISLCYCHAAGKLLPPGAGRFLAVAPVVALFLLLPLQLTSIHLGG 60
           MEGEI NF  VWL VC+SLCYCH AGK+L PGAGRFLA++PVV LF LLPLQLTS+HLGG
Sbjct: 1   MEGEIINFTMVWLIVCMSLCYCHTAGKILHPGAGRFLAISPVVLLFFLLPLQLTSVHLGG 60

Query: 61  STSFFVAWLANFKLLLFAAGDGPLSAAPPLSLRRFLALSCLPIKILQNPSEISSPNNRAE 120
           +T+FF+AWLANFKLLLFAAG+GPLSA PPL LRRFLALSCLPIKILQNPSE  SP N  E
Sbjct: 61  ATAFFIAWLANFKLLLFAAGNGPLSATPPLPLRRFLALSCLPIKILQNPSEKQSPKNHHE 120

Query: 121 TLTLRPKSFGNYAIKALIVALMVKSYDYSEFLHPKLVLFIYSWHVYFLLEIILAITQFLG 180
           TL   PKSF NY IKALIVALMVK YDYSEF+HPK++LFIYSWHVYF LEIILAITQF G
Sbjct: 121 TLNQEPKSFLNYWIKALIVALMVKLYDYSEFIHPKVILFIYSWHVYFFLEIILAITQFFG 180

Query: 181 RNLLGIELEPQFNDPYFSTSLQDFWGRRWNLMATNILQPTVYKPSVKIAAKVVGRKWAPL 240
           R LLGIELEPQF+DPYFSTSLQDFWGRRWNLMATNILQPTVYKP+VKIAAKVVGRKWAPL
Sbjct: 181 RTLLGIELEPQFHDPYFSTSLQDFWGRRWNLMATNILQPTVYKPTVKIAAKVVGRKWAPL 240

Query: 241 PAVMATFMVSAMMHELIFYYLGRMRPNWEITWFFVIHGVALTVEILMKKALPEKWRVPAV 300
           P VMATF+VSA+MHELIFYYLGRM+PNWE+TWFFVIHG ALTVEIL+KK LPEK R+PA 
Sbjct: 241 PGVMATFLVSAIMHELIFYYLGRMKPNWELTWFFVIHGFALTVEILVKKVLPEKRRLPAA 300

Query: 301 VSVPVTVGFIFWTAIWLFFPQFVRLKIDVRAFEEYAALVTFVKKLAFSLVS 352
           VSVPVT+ FIF TAIWLFFPQF+RLKIDV+AFEEYAALV+F+KKLAF LVS
Sbjct: 301 VSVPVTLVFIFSTAIWLFFPQFMRLKIDVKAFEEYAALVSFMKKLAFPLVS 351

[Close]

BLAST of Lag0031126 vs. ExPASy TrEMBL
Match: A0A0A0L9S2 (MBOAT_2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G684690 PE=3 SV=1)

HSP 1 Score: 563.1 bits (1450), Expect = 7.9e-157
Identity = 283/351 (80.63%), Postives = 305/351 (86.89%), Query Frame = 0


Query: 1   MEGEIFNFVKVWLAVCISLCYCHAAGKLLPPGAGRFLAVAPVVALFLLLPLQLTSIHLGG 60
           ME EI N  KVWL V ISL YCHA  KLL PG  RFLA+APVV +F LLPLQLTSIH GG
Sbjct: 28  MEAEIINLTKVWLTVFISLGYCHATAKLLNPGPARFLAIAPVVLIFFLLPLQLTSIHFGG 87

Query: 61  STSFFVAWLANFKLLLFAAGDGPLSAAPPLSLRRFLALSCLPIKILQNPSEISSP-NNRA 120
           +T FFV WLA FKLLL  AGDGPLS  PPL L RF+A+SCLPIKIL+NPSE S+P NNRA
Sbjct: 88  ATCFFVTWLATFKLLLLTAGDGPLSTTPPLPLHRFIAISCLPIKILENPSEKSAPKNNRA 147

Query: 121 ETLTLRPKSFGNYAIKALIVALMVKSYDYSEFLHPKLVLFIYSWHVYFLLEIILAITQFL 180
           ETL  +PKSF NY+I+ALIV LMVK YDYS F HPKLVLFIYSWHVY LLEIILAITQF 
Sbjct: 148 ETLNEKPKSFLNYSIRALIVVLMVKLYDYSHFFHPKLVLFIYSWHVYLLLEIILAITQFF 207

Query: 181 GRNLLGIELEPQFNDPYFSTSLQDFWGRRWNLMATNILQPTVYKPSVKIAAKVVGRKWAP 240
           GRNLLGIELEPQF DPY+STSLQDFWGRRWNLMATNILQP VYKP+VKIAA+V+GR WAP
Sbjct: 208 GRNLLGIELEPQFRDPYYSTSLQDFWGRRWNLMATNILQPAVYKPTVKIAARVIGRMWAP 267

Query: 241 LPAVMATFMVSAMMHELIFYYLGRMRPNWEITWFFVIHGVALTVEILMKKALPEKWRVPA 300
           LP VMATF+VSAMMHELIFYYLGRMRPNWEITWFFVIHG+ALTVEI+MKK LPEK R+PA
Sbjct: 268 LPGVMATFLVSAMMHELIFYYLGRMRPNWEITWFFVIHGIALTVEIMMKKMLPEKRRLPA 327

Query: 301 VVSVPVTVGFIFWTAIWLFFPQFVRLKIDVRAFEEYAALVTFVKKLAFSLV 351
            VSVPVT+ FIF TAIWLFFPQFVRL++DVRAFEEYAAL  FVKK+AFSLV
Sbjct: 328 KVSVPVTLVFIFSTAIWLFFPQFVRLRLDVRAFEEYAALANFVKKVAFSLV 378

[Close]

BLAST of Lag0031126 vs. TAIR 10
Match: AT3G51970.1 (acyl-CoA sterol acyl transferase 1 )

HSP 1 Score: 317.4 bits (812), Expect = 1.5e-86
Identity = 168/346 (48.55%), Postives = 233/346 (67.34%), Query Frame = 0


Query: 7   NFVKVWLAVCISLCYCHAAGKLLPPGAGRFLAVAPVVALFLLLPLQLTSIHLGGSTSFFV 66
           +F+K W  V ISLCY     KL+P G  R +   PV  +F ++P  + S+HL G T+FF+
Sbjct: 3   SFIKAWGLVIISLCYTFFIAKLVPKGIKRLILFFPVFLIFFIVPFLIYSLHLLGITAFFI 62

Query: 67  AWLANFKLLLFAAGDGPLSA-APPLSLRRFLALSCLPIKILQNPSEISSPNNRAETLTLR 126
           AWLANFKLLLFA G GPLS+   PLSL  FLA+SCLPIKI  +P    + ++   T    
Sbjct: 63  AWLANFKLLLFALGRGPLSSNHKPLSLPIFLAVSCLPIKIQLSPKPTKTHSHEGST---- 122

Query: 127 PKSFGNYAIKALIVALMVKSYDYSEFLHPKLVLFIYSWHVYFLLEIILAITQFLGRNLLG 186
            +    Y IKA+ V L++K+Y+YS  L  K+VL +Y+ H+YF LEIILA T    R +  
Sbjct: 123 -EGPLIYTIKAVFVVLIIKAYEYSTKLPEKVVLTLYAIHIYFALEIILAATAAAVRAMSD 182

Query: 187 IELEPQFNDPYFSTSLQDFWGRRWNLMATNILQPTVYKPSVKIAAKVVGRKWAPLPAVMA 246
           +ELEPQFN PY +TSLQDFWGRRWNLM T IL+PTVY+PS+++ + V+G  ++ + A   
Sbjct: 183 LELEPQFNKPYLATSLQDFWGRRWNLMVTGILRPTVYEPSLQLFS-VLGPNYSQILAAFG 242

Query: 247 TFMVSAMMHELIFYYLGRMRPNWEITWFFVIHGVALTVEILMKKALPEKWRVPAVVSVPV 306
           TF+VS +MHELIF+Y+GR+RP+W++ WFF+I+G   TVEI +KK +  +WR P  +S  +
Sbjct: 243 TFVVSGIMHELIFFYMGRLRPDWKMMWFFLINGFCTTVEIAIKKTINGRWRFPKAISQVL 302

Query: 307 TVGFIFWTAIWLFFPQFVRLKIDVRAFEEYAALVTFVKKLAFSLVS 352
           T+ F+  TA+WLF P+F R  I  +A +EYAA+  F  ++   L +
Sbjct: 303 TLTFVMVTALWLFLPEFNRCNIVEKALDEYAAIGAFAVEVRRKLTA 342

[Close]

BLAST of Lag0031126 vs. TAIR 10
Match: AT5G55340.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 272.3 bits (695), Expect = 5.4e-73
Identity = 150/346 (43.35%), Postives = 214/346 (61.85%), Query Frame = 0


Query: 1   MEGEIFNFVKVWLAVCISLCYCHAAGKLLPPGAGRFLAVAPVVALFLLLPLQLTSIHLGG 60
           M+ E+ N +KVW++  IS+ YC+     +  GA RFL+V+PV+ALFL+LPL  +S+HL  
Sbjct: 1   MDEELKNLIKVWVSAIISISYCYYIPPRIKSGAPRFLSVSPVLALFLVLPLFFSSLHLSL 60

Query: 61  STSFFVAWLANFKLLLFAAGDGPLSAAPPLSLRRFLALSCLPIKILQNPSEISSPNNRAE 120
            T+FF+ WLANFKL+LF+   GPL    P +  RF   +C PIK+ QNP    S N+  +
Sbjct: 61  ITAFFLTWLANFKLILFSFDKGPLIPI-PTNFPRFFCFTCFPIKVQQNP---KSQNHLPK 120

Query: 121 TLTLRPKSFGNYAIKALIVALMVKSYDYSEFLHPKLVLFIYSWHVYFLLEIILAITQFLG 180
            +         +AIK  I A+++  Y Y + L P ++L +Y  H+Y  +EIIL   + + 
Sbjct: 121 LV---------FAIKLAIFAVLLHLYSYRQNLSPTILLGLYFVHLYLEIEIILTFVKVVV 180

Query: 181 RNLLGIELEPQFNDPYFSTSLQDFWGRRWNLMATNILQPTVYKPSVKIAAKVVGRKWAPL 240
              LG +LEPQ N PY +TSLQDFWGRRWNLM   IL+P VY P  +++ + +   WA  
Sbjct: 181 FISLGCDLEPQSNKPYLATSLQDFWGRRWNLMVPAILRPAVYAPMRRVSERKMSSGWALF 240

Query: 241 PAVMATFMVSAMMHELIFYYLGRMRPNWEITWFFVIHGVALTVEILMKK--ALPEKWRVP 300
           P ++A F+VS ++HEL+F+YL R  P  E+T FFV+HGV   VE+ +KK   + ++WR+ 
Sbjct: 241 PGILAAFIVSGLVHELLFFYLIREMPTGEVTLFFVLHGVCTAVELAVKKKTTVAQRWRLS 300

Query: 301 AVVSVPVTVGFIFWTAIWLFFPQFVRLKIDVRAFEEYAALVTFVKK 345
             VS  +TVGF+F T  WLF PQ  R  +  R   E   LV F+K+
Sbjct: 301 PGVSRVLTVGFVFVTGGWLFTPQLKRSGVMERFTSEAVLLVEFIKR 333

[Close]

BLAST of Lag0031126 vs. TAIR 10
Match: AT5G55350.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 263.1 bits (671), Expect = 3.3e-70
Identity = 148/350 (42.29%), Postives = 207/350 (59.14%), Query Frame = 0


Query: 1   MEGEIFNFVKVWLAVCISLCYCHAAGKLLPPGAGRFLAVAPVVALFLLLPLQLTSIHLGG 60
           ME E+ + +KVW+   IS+ YC+     +  G  R L+V PV  LFL+LPL ++S+H  G
Sbjct: 1   MEEELMSLIKVWVYAIISISYCYYTSTRIKSGVFRLLSVLPVCVLFLVLPLFVSSVHFSG 60

Query: 61  STSFFVAWLANFKLLLFAAGDGPLSAAPPLSLRRFLALSCLPIKILQNPSEISSPNNRAE 120
           ST+FF++WLANFKL+LF+   GPL   P  +L RF+  +C PIK+ QNP     P N+  
Sbjct: 61  STAFFLSWLANFKLILFSFDQGPLFPVPS-NLSRFVCFTCFPIKLQQNP----KPQNQ-- 120

Query: 121 TLTLRPKSFGNYAIKALIVALMVKSYDYSEFLHPKLVLFIYSWHVYFLLEIILAITQFLG 180
                PK    +A+K     +++  Y+Y + + P ++L +YS H+Y   EI+LA  + L 
Sbjct: 121 ----MPK--WGFAVKLAFFGVLLHMYEYKQHMSPTVLLVLYSLHIYLEYEILLAPLKVLL 180

Query: 181 RNLLGIELEPQFNDPYFSTSLQDFWGRRWNLMATNILQPTVYKPSVKIAAKVVGRKWAPL 240
              L  +LEP FN+PY STSLQDFWGRRWNLM   IL+P VY P  ++A + +    A  
Sbjct: 181 SISLWCDLEPHFNEPYLSTSLQDFWGRRWNLMVPAILRPAVYLPVRQMAGRKMNSDQALF 240

Query: 241 PAVMATFMVSAMMHELIFYYLGRMRPNWEITWFFVIHGVALTVEILMKKA-LPEKWRVPA 300
             V A+F+VS ++HELIF+Y  R  P  E+T FFV+HGV    E   K+  L  +W+V  
Sbjct: 241 LGVFASFLVSGVVHELIFFYFTRESPTGEVTLFFVLHGVCTAAECAAKRTRLVRRWKVSQ 300

Query: 301 VVSVPVTVGFIFWTAIWLFFPQFVRLKIDVRAFEEYAALVTFVKKLAFSL 350
           +VS  +TVGF+  T  WLFFP   R  +  R  +E    + FVK   F L
Sbjct: 301 MVSRLLTVGFVVMTGGWLFFPHLARSGMIERLADEAFLFIGFVKHKFFYL 337

[Close]

BLAST of Lag0031126 vs. TAIR 10
Match: AT5G55360.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 258.8 bits (660), Expect = 6.1e-69
Identity = 145/345 (42.03%), Postives = 208/345 (60.29%), Query Frame = 0


Query: 1   MEGEIFNFVKVWLAVCISLCYCHAAGKLLPPGAGRFLAVAPVVALFLLLPLQLTSIHLGG 60
           ME E+ NF+K+W++  IS+ YC+     +  G  R L+V PV ALFL+ PL  + +H  G
Sbjct: 1   MEEELKNFIKLWISAIISISYCYYLSTGIKAGVFRLLSVLPVCALFLVFPLFFSYVHFSG 60

Query: 61  STSFFVAWLANFKLLLFAAGDGPLSAAPPLSLRRFLALSCLPIKILQNPSEISSPNNRAE 120
             +FF++WLANFKL+LF+   GPLS   P +L RF+ ++C PIK  QNP    +  N   
Sbjct: 61  CMAFFLSWLANFKLILFSFDQGPLSPL-PRTLSRFICITCFPIKPQQNP----NIQNYKI 120

Query: 121 TLTLRPKSFGNYAIKALIVALMVKSYDYSEFLHPKLVLFIYSWHVYFLLEIILAITQFLG 180
            + L       +AIK +I  ++++ Y+Y ++L P L+L   S H++  LEI+  + + L 
Sbjct: 121 PIWL-------FAIKVVIFVVLLQMYEYKQYLSPALLLVFNSLHIFLELEIVFMLVKALV 180

Query: 181 RNLLGIELEPQFNDPYFSTSLQDFWGRRWNLMATNILQPTVYKPSVKIAAKVVGRKWAPL 240
              LG +LEPQ N+PY +TSLQDFWGRRWNLM   IL+P VY P+ ++A + V    A  
Sbjct: 181 FITLGCDLEPQSNEPYLATSLQDFWGRRWNLMVPAILRPAVYLPARRMACRKVNSDQAMF 240

Query: 241 PAVMATFMVSAMMHELIFYYLGRMRPNWEITWFFVIHGVALTVEILMKKA--LPEKWRVP 300
             V A F+VS  +HE++F+YL R  P  E+TWFF++HGV    E+ +KK+  +   WRV 
Sbjct: 241 LGVFAAFLVSGAVHEMLFFYLTREVPTGEVTWFFLLHGVCTVAEVAVKKSTFVRRWWRVS 300

Query: 301 AVVSVPVTVGFIFWTAIWLFFPQFVRLKIDVRAFEEYAALVTFVK 344
             VS  +TVGF+  T+ W FFP  +R  I  R   E    + FVK
Sbjct: 301 PTVSRLLTVGFVVVTSGWFFFP-LIRSGIIERLASEALMCIDFVK 332

[Close]

BLAST of Lag0031126 vs. TAIR 10
Match: AT5G55380.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 246.5 bits (628), Expect = 3.1e-65
Identity = 138/347 (39.77%), Postives = 203/347 (58.50%), Query Frame = 0


Query: 1   MEGEIFNFVKVWLAVCISLCYCHAAGKLLPPGAGRFLAVAPVVALFLLLPLQLTSIHLGG 60
           ME +  N ++VW++  ISL YC+     L  G  R L++ PV  LFL+LPL L+ +H   
Sbjct: 1   MEEKFRNLIEVWISALISLSYCYYISSKLSKGVLRLLSILPVCILFLVLPLFLSCVHFCA 60

Query: 61  STSFFVAWLANFKLLLFAAGDGPLSAAPPLSLRRFLALSCLPIKILQNPSEISSPNNRAE 120
            +  F++WLANFKLLLFA  +GPL   PP  L RF+  +CLPIKI Q+PS  + PN    
Sbjct: 61  ISVLFLSWLANFKLLLFAFDEGPLFPLPP-KLSRFICFACLPIKIRQDPSPNAIPN---- 120

Query: 121 TLTLRPKSFGNY--AIKALIVALMVKSYDYSEFLHPKLVLFIYSWHVYFLLEIILAITQF 180
              L PK    +  A+K L++ +++  Y+Y + L   +VL +Y  H+Y  +E++L     
Sbjct: 121 ---LHPKPMPKWVLAVKILVLGVLLHVYEYRDGLPRFVVLALYCLHIYLEVELVLVFVGA 180

Query: 181 LGRNLLGIELEPQFNDPYFSTSLQDFWGRRWNLMATNILQPTVYKPSVKIAAKVVGRKWA 240
           +   LLG  +EP FN+PY +TSLQDFW RRWNLM + +L+ TV+ P  +   +++    A
Sbjct: 181 VVSTLLGCNIEPVFNEPYLATSLQDFWSRRWNLMVSAVLRSTVHIPVQRFFKRILSPDGA 240

Query: 241 PLPAVMATFMVSAMMHELIFYYLGRMRPNWEITWFFVIHGVALTVEILMKKALPEKWRVP 300
               VMA+F VS +MHEL+++Y+ R  P WE+T FFV+HG A   EI +K+    +W  P
Sbjct: 241 MFAGVMASFFVSGLMHELLYFYMIRKPPTWEVTCFFVLHGAATATEIAVKRT---QWLRP 300

Query: 301 --AVVSVPVTVGFIFWTAIWLFFPQFVRLKIDVRAFEEYAALVTFVK 344
               VS  V + F+  T +WLF  Q +R  +  +A  E   ++   K
Sbjct: 301 PHRAVSGLVVLTFVSVTGVWLFLAQVLRNNVHEKAIGECLLVLDLAK 336

The following BLAST results are available for this feature:

BLAST of Lag0031126 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022948970.1	5.8e-170	86.29	acyl-CoA--sterol O-acyltransferase 1-like [Cucurbita moschata] >KAG6606895.1 Acy...	[more]
XP_022998466.1	7.5e-170	85.71	acyl-CoA--sterol O-acyltransferase 1-like [Cucurbita maxima]	[more]
XP_022934183.1	1.6e-167	83.76	acyl-CoA--sterol O-acyltransferase 1-like [Cucurbita moschata]	[more]
XP_023527814.1	2.7e-167	84.05	acyl-CoA--sterol O-acyltransferase 1-like [Cucurbita pepo subsp. pepo]	[more]
XP_023523550.1	2.3e-166	85.30	LOW QUALITY PROTEIN: acyl-CoA--sterol O-acyltransferase 1-like [Cucurbita pepo s...	[more]

BLAST of Lag0031126 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SV07	2.0e-85	48.55	Acyl-CoA--sterol O-acyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=ASAT1 PE...	[more]
Q9XGY6	3.4e-72	42.45	Long-chain-alcohol O-fatty-acyltransferase OS=Simmondsia chinensis OX=3999 PE=1 ...	[more]
Q9FJ76	7.5e-72	43.35	Probable long-chain-alcohol O-fatty-acyltransferase 5 OS=Arabidopsis thaliana OX...	[more]
Q9FJ75	4.6e-69	42.29	Probable long-chain-alcohol O-fatty-acyltransferase 4 OS=Arabidopsis thaliana OX...	[more]
Q9FJ74	8.6e-68	42.03	Probable long-chain-alcohol O-fatty-acyltransferase 3 OS=Arabidopsis thaliana OX...	[more]

BLAST of Lag0031126 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GBE5	2.8e-170	86.29	acyl-CoA--sterol O-acyltransferase 1-like OS=Cucurbita moschata OX=3662 GN=LOC11...	[more]
A0A6J1KAA2	3.7e-170	85.71	acyl-CoA--sterol O-acyltransferase 1-like OS=Cucurbita maxima OX=3661 GN=LOC1114...	[more]
A0A6J1F6Y6	7.6e-168	83.76	acyl-CoA--sterol O-acyltransferase 1-like OS=Cucurbita moschata OX=3662 GN=LOC11...	[more]
A0A6J1J5C4	4.2e-166	83.76	acyl-CoA--sterol O-acyltransferase 1-like OS=Cucurbita maxima OX=3661 GN=LOC1114...	[more]
A0A0A0L9S2	7.9e-157	80.63	MBOAT_2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G684690 PE=...	[more]

BLAST of Lag0031126 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G51970.1	1.5e-86	48.55	acyl-CoA sterol acyl transferase 1	[more]
AT5G55340.1	5.4e-73	43.35	MBOAT (membrane bound O-acyl transferase) family protein	[more]
AT5G55350.1	3.3e-70	42.29	MBOAT (membrane bound O-acyl transferase) family protein	[more]
AT5G55360.1	6.1e-69	42.03	MBOAT (membrane bound O-acyl transferase) family protein	[more]
AT5G55380.1	3.1e-65	39.77	MBOAT (membrane bound O-acyl transferase) family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR017088	Wax synthase, Magnoliopsida	PIRSF	PIRSF037006	Wax_synthase	coord: 1..351 e-value: 6.7E-120 score: 398.3
IPR032805	Wax synthase domain	PFAM	PF13813	MBOAT_2	coord: 189..275 e-value: 1.1E-15 score: 57.7
None	No IPR available	PANTHER	PTHR31595:SF46	ACYL-COA--STEROL O-ACYLTRANSFERASE 1	coord: 2..346
IPR044851	Wax synthase	PANTHER	PTHR31595	LONG-CHAIN-ALCOHOL O-FATTY-ACYLTRANSFERASE 3-RELATED	coord: 2..346

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0031126.1	Lag0031126.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006629	lipid metabolic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0008374	O-acyltransferase activity