Lag0031124 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0031124
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	acyl-CoA--sterol O-acyltransferase 1-like
Location	chr11: 5039600 .. 5040714 (-)
RNA-Seq Expression	Lag0031124
Synteny	Lag0031124

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAAGGTGAGATCATGAACTTCATCCAAGTGTGGCTCACAGTCTCTGCATCTCTCTGTTACTGCCACCTCATCGGAAACCATGTCCGACCAGGCCGCCGCCGCCTGATCTCCATCCTCCCCGTCGTCTGTCTCTTCCTCTACCTCCCTCTCCGCCTCTCCTCCGCCCATCTCGGCGGCGCCACCGCCTTCTTCGTCGCTTGGCTTGCCAATTTCAAGCTCCTCCTCTTCTCCTTCAACCAAGGCCCTTTATCTTCCCATCCGCCTCTGCCTCTCCGCCGATTCCTCGCTGCTTCCTGTTTGCCGATCAAAATCCTTCAAAACCCTTCTCGAAATTCACAAAATGGCGAATACCCATCTCCCAAATCGGGCCCAAATCGCCGGCAAATCCAAAATCAAGAACACCCAATTCAGAAATCGTTCCTCAGCTACGCAATCAAAGCTCTGTTCGTGGCATTCATGGTGAAACTCTACGATTACAGTGAACTTATGAACCAGAAAGTGATTTTAGCCATCTACAGTTTGCACGTTTACTTTCTCCTCGAACTGATCCTCGCCGCCGCGAAATTCCTCGCCCGGAATCTCATCGGCGCCGAGCTCGAGCCGCAGTTCAACGATCCGTTTTTCTCCACTTCGCTTCAGGACTTTTGGGGCGGAGATGGAATCTGATGGTGACGGGGATTTTGAATCCGACGGTGTACAAACCGACGCTCCGGCTGGTCTCGCCCGTTGTCGGCCGGAAGTGGGCGCCACTGCCGGCGGTGTTGGCGACGTTTGGGGTGTCGGCGTTGATGCATGAGCTTATTTTTTTCTACTTGGGCCGGAAGAGGCCCGATTGGGGAATTTCGATGTTTTTTGTGGTTCATGGGGTGGCTTTGACGGCGGAGATTTTGGCGAAGAAGACTTTGCCGGCGAAATGGCGGCTGCCGGCGGCGGTGTCGGGGCCGTTGACGGTGGTGTTTGTGTTCGTCACCGGAATTTGGTTGTTCTTCCCGCAGTTCGTACGGTGTGGGATCGACGTTAAGGCGTTTGCCGAGTACGCGGCGGTCGGCGATTTTGTGAGACGATGTGTCGTTCTAATCGGCGAGTTGTTGGGATTTGATTTGAATTTCTGA

mRNA sequence

ATGGAAGGTGAGATCATGAACTTCATCCAAGTGTGGCTCACAGTCTCTGCATCTCTCTGTTACTGCCACCTCATCGGAAACCATGTCCGACCAGGCCGCCGCCGCCTGATCTCCATCCTCCCCGTCGTCTGTCTCTTCCTCTACCTCCCTCTCCGCCTCTCCTCCGCCCATCTCGGCGGCGCCACCGCCTTCTTCGTCGCTTGGCTTGCCAATTTCAAGCTCCTCCTCTTCTCCTTCAACCAAGGCCCTTTATCTTCCCATCCGCCTCTGCCTCTCCGCCGATTCCTCGCTGCTTCCTGTTTGCCGATCAAAATCCTTCAAAACCCTTCTCGAAATTCACAAAATGGCGAATACCCATCTCCCAAATCGGGCCCAAATCGCCGGCAAATCCAAAATCAAGAACACCCAATTCAGAAATCGTTCCTCAGCTACGCAATCAAAGCTCTGTTCGTGGCATTCATGTTTGCACGTTTACTTTCTCCTCGAACTGATCCTCGCCGCCGCGAAATTCCTCGCCCGGAATCTCATCGGCGCCGAGCTCGAGCCGCAGTTCAACGATCCGTTTTTCTCCACTTCGCTTCAGGACTTTTGGGGCGGAGATGGAATCTGATGGTGACGGGGATTTTGAATCCGACGGTGTACAAACCGACGCTCCGGCTGGTCTCGCCCGTTGTCGGCCGGAAGTGGGCGCCACTGCCGGCGGTGTTGGCGACGTTTGGGGTGTCGGCGTTGATGCATGAGCTTATTTTTTTCTACTTGGGCCGGAAGAGGCCCGATTGGGGAATTTCGATGTTTTTTGTGGTTCATGGGGTGGCTTTGACGGCGGAGATTTTGGCGAAGAAGACTTTGCCGGCGAAATGGCGGCTGCCGGCGGCGGTGTCGGGGCCGTTGACGGTGGTGTTTGTGTTCGTCACCGGAATTTGGTTGTTCTTCCCGCAGTTCGTACGGTGTGGGATCGACGTTAAGGCGTTTGCCGAGTACGCGGCGGTCGGCGATTTTGTGAGACGATGTGTCGTTCTAATCGGCGAGTTGTTGGGATTTGATTTGAATTTCTGA

Coding sequence (CDS)

ATGGAAGGTGAGATCATGAACTTCATCCAAGTGTGGCTCACAGTCTCTGCATCTCTCTGTTACTGCCACCTCATCGGAAACCATGTCCGACCAGGCCGCCGCCGCCTGATCTCCATCCTCCCCGTCGTCTGTCTCTTCCTCTACCTCCCTCTCCGCCTCTCCTCCGCCCATCTCGGCGGCGCCACCGCCTTCTTCGTCGCTTGGCTTGCCAATTTCAAGCTCCTCCTCTTCTCCTTCAACCAAGGCCCTTTATCTTCCCATCCGCCTCTGCCTCTCCGCCGATTCCTCGCTGCTTCCTGTTTGCCGATCAAAATCCTTCAAAACCCTTCTCGAAATTCACAAAATGGCGAATACCCATCTCCCAAATCGGGCCCAAATCGCCGGCAAATCCAAAATCAAGAACACCCAATTCAGAAATCGTTCCTCAGCTACGCAATCAAAGCTCTGTTCGTGGCATTCATGTTTGCACGTTTACTTTCTCCTCGAACTGATCCTCGCCGCCGCGAAATTCCTCGCCCGGAATCTCATCGGCGCCGAGCTCGAGCCGCAGTTCAACGATCCGTTTTTCTCCACTTCGCTTCAGGACTTTTGGGGCGGAGATGGAATCTGATGGTGACGGGGATTTTGAATCCGACGGTGTACAAACCGACGCTCCGGCTGGTCTCGCCCGTTGTCGGCCGGAAGTGGGCGCCACTGCCGGCGGTGTTGGCGACGTTTGGGGTGTCGGCGTTGATGCATGAGCTTATTTTTTTCTACTTGGGCCGGAAGAGGCCCGATTGGGGAATTTCGATGTTTTTTGTGGTTCATGGGGTGGCTTTGACGGCGGAGATTTTGGCGAAGAAGACTTTGCCGGCGAAATGGCGGCTGCCGGCGGCGGTGTCGGGGCCGTTGACGGTGGTGTTTGTGTTCGTCACCGGAATTTGGTTGTTCTTCCCGCAGTTCGTACGGTGTGGGATCGACGTTAAGGCGTTTGCCGAGTACGCGGCGGTCGGCGATTTTGTGAGACGATGTGTCGTTCTAATCGGCGAGTTGTTGGGATTTGATTTGAATTTCTGA

Protein sequence

MEGEIMNFIQVWLTVSASLCYCHLIGNHVRPGRRRLISILPVVCLFLYLPLRLSSAHLGGATAFFVAWLANFKLLLFSFNQGPLSSHPPLPLRRFLAASCLPIKILQNPSRNSQNGEYPSPKSGPNRRQIQNQEHPIQKSFLSYAIKALFVAFMFARLLSPRTDPRRREIPRPESHRRRARAAVQRSVFLHFASGLLGRRWNLMVTGILNPTVYKPTLRLVSPVVGRKWAPLPAVLATFGVSALMHELIFFYLGRKRPDWGISMFFVVHGVALTAEILAKKTLPAKWRLPAAVSGPLTVVFVFVTGIWLFFPQFVRCGIDVKAFAEYAAVGDFVRRCVVLIGELLGFDLNF

Homology

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BLAST of Lag0031124 vs. NCBI nr
Match: XP_022152669.1 (acyl-CoA--sterol O-acyltransferase 1-like [Momordica charantia])

HSP 1 Score: 429.9 bits (1104), Expect = 2.2e-116
Identity = 242/367 (65.94%), Postives = 268/367 (73.02%), Query Frame = 0


Query: 1   MEGEIMNFIQVWLTVSASLCYCHLIGNHVRPGRRRLISILPVVCLFLYLPLRLSSAHLGG 60
           ME EI+NFI VWLTV ASLCYC+LIG +VRPG RRLISI PVVCLFLYLPLRLSSAHLGG
Sbjct: 1   MENEIINFINVWLTVCASLCYCYLIGKNVRPGGRRLISIFPVVCLFLYLPLRLSSAHLGG 60

Query: 61  ATAFFVAWLANFKLLLFSFNQGPLSSHPPLPLRRFLAASCLPIKILQNPSRNSQNGEYPS 120
            TAFF+AWLANFKLLLFSF  GPLSS PPLPLRRFLA SCLPIKI +N  + SQNGE  S
Sbjct: 61  PTAFFIAWLANFKLLLFSFAGGPLSSDPPLPLRRFLAVSCLPIKIQRNHLQISQNGENTS 120

Query: 121 PKSGPNRRQIQNQEHPI-QKSFLSYAIKALFVAFM-----FARLLSPRTDP--------- 180
           PKS PN  QI   E P+  KSFLSYAIK   VA M     ++  + P+            
Sbjct: 121 PKSSPNAPQI---ETPLPSKSFLSYAIKGFLVALMVKLYDYSEFMHPKLVMCIYSWHIYF 180

Query: 181 -RRREIPRPESHRRRARAAVQRSVF--LHFASGL---LGRRWNLMVTGILNPTVYKPTLR 240
                +   +   R    A     F   +F++ L    GRRWNLMVTGILNPTVYKPT+R
Sbjct: 181 LLEIILAAAKFLARNLLGAELEPQFNDPYFSTSLQDFWGRRWNLMVTGILNPTVYKPTIR 240

Query: 241 LVSPVVGRKWAPLPAVLATFGVSALMHELIFFYLGRKRPDWGISMFFVVHGVALTAEILA 300
           L +PVVGRKWAPL AVLATF VSA+MHELIF+YLGR RPDW +S FF VHGVALT EILA
Sbjct: 241 LAAPVVGRKWAPLLAVLATFFVSAMMHELIFYYLGRMRPDWEVSRFFAVHGVALTVEILA 300

Query: 301 KKTLPAKW-RLPAAVSGPLTVVFVFVTGIWLFFPQFVRCGIDVKAFAEYAAVGDFVRRCV 346
           KK  P K  R+PA VS PLTV FVFVTGIW+FFPQFVRCGIDVKAFAEYAAV DF  + +
Sbjct: 301 KKAWPEKCRRVPAVVSVPLTVGFVFVTGIWMFFPQFVRCGIDVKAFAEYAAVSDFFTKRL 360

[Close]

BLAST of Lag0031124 vs. NCBI nr
Match: XP_022998466.1 (acyl-CoA--sterol O-acyltransferase 1-like [Cucurbita maxima])

HSP 1 Score: 354.8 bits (909), Expect = 8.8e-94
Identity = 190/360 (52.78%), Postives = 234/360 (65.00%), Query Frame = 0


Query: 1   MEGEIMNFIQVWLTVSASLCYCHLIGNHVRPGRRRLISILPVVCLFLYLPLRLSSAHLGG 60
           MEGEI+NFI+VWLTV  SLCYCH  G  + PG  R +++ P+V LF +LPL+L+S HLGG
Sbjct: 1   MEGEILNFIKVWLTVCISLCYCHATGKLIPPGAGRFLAVAPIVVLFFFLPLQLTSIHLGG 60

Query: 61  ATAFFVAWLANFKLLLFSFNQGPLSSHPPLPLRRFLAASCLPIKILQNPSRNSQNGEYPS 120
           +T+FF+AWLANFKLLLF+   GPLS+ PPLPLRRFLA SCLPIKILQN            
Sbjct: 61  STSFFIAWLANFKLLLFTAGDGPLSAEPPLPLRRFLALSCLPIKILQN------------ 120

Query: 121 PKSGPNRRQIQNQEHPIQKSFLSYAIKALFVAFM-----FARLLSPR------------- 180
            KS PN  +      P  KSFL+Y+IKA+ VA M     ++  + P+             
Sbjct: 121 EKSCPNNNRSDTTLKP--KSFLNYSIKAVIVALMVKLYDYSEFIHPKVVLFIYSWHLYFL 180

Query: 181 -------TDPRRREIPRPESHRRRARAAVQRSVFLHFASGLLGRRWNLMVTGILNPTVYK 240
                  T    R +   E          Q   F        GRRWNLM T IL PTVYK
Sbjct: 181 LEIILAITQFFGRNVLGIE-----LEPQFQDPYFSTSLQDFWGRRWNLMATNILQPTVYK 240

Query: 241 PTLRLVSPVVGRKWAPLPAVLATFGVSALMHELIFFYLGRKRPDWGISMFFVVHGVALTA 300
           P++++ + V+GRKWAPLPAV+ATF VSA+MHELIFFY+GR RP+W  + FFV+HGVALT 
Sbjct: 241 PSVKIAAKVIGRKWAPLPAVMATFLVSAMMHELIFFYMGRMRPNWECTWFFVIHGVALTV 300

Query: 301 EILAKKTLPAKWRLPAAVSGPLTVVFVFVTGIWLFFPQFVRCGIDVKAFAEYAAVGDFVR 336
           EI+ KK LP KWRLP AVS P+T+ F+F T IWLFFPQFVR  +DV+AF EYAA+  FV+
Sbjct: 301 EIMVKKVLPEKWRLPPAVSVPVTLAFIFPTAIWLFFPQFVRLKLDVRAFEEYAALVTFVK 341

[Close]

BLAST of Lag0031124 vs. NCBI nr
Match: XP_022934183.1 (acyl-CoA--sterol O-acyltransferase 1-like [Cucurbita moschata])

HSP 1 Score: 354.8 bits (909), Expect = 8.8e-94
Identity = 197/356 (55.34%), Postives = 239/356 (67.13%), Query Frame = 0


Query: 1   MEGEIMNFIQVWLTVSASLCYCHLIGNHVRPGRRRLISILPVVCLFLYLPLRLSSAHLGG 60
           MEGE++NFI VWL V  SLCYCH  G  + PG  R ++I PVV LF  LPL+L+S HLGG
Sbjct: 1   MEGEVINFIMVWLIVCMSLCYCHTAGKILPPGAGRFLAIAPVVVLFFLLPLQLTSVHLGG 60

Query: 61  ATAFFVAWLANFKLLLFSFNQGPLSSHPPLPLRRFLAASCLPIKILQNPSRNSQNGEYPS 120
           +TAFF+AWLANFKLLLF+   GPLS+ PPLPLRRFLA SCLPIKILQNPS      E  S
Sbjct: 61  STAFFIAWLANFKLLLFTAGNGPLSATPPLPLRRFLALSCLPIKILQNPS------EKQS 120

Query: 121 PKSGPNRRQIQNQEHPIQKSFLSYAIKALFVAFM-----FARLLSPRTDP---------R 180
           PK   N  +  NQE    KSFL+Y IKAL VA M     ++  + P+             
Sbjct: 121 PK---NHHETLNQE---PKSFLNYWIKALIVALMVKLYDYSEFIHPKVILFIYSWHVYFF 180

Query: 181 RREIPRPESHRRRARAAVQRSVFLH---FASGL---LGRRWNLMVTGILNPTVYKPTLRL 240
              I        R    ++     H   F++ L    GRRWNLM T IL PT+YKPT+++
Sbjct: 181 LEIILAITQFFGRTLLGIELEPQFHDPYFSTSLQDFWGRRWNLMATNILQPTIYKPTVKI 240

Query: 241 VSPVVGRKWAPLPAVLATFGVSALMHELIFFYLGRKRPDWGISMFFVVHGVALTAEILAK 300
            + VVGRKWAPLP V+ATF VSA+MHELIF+YLGR +P+W ++ FFV+HG ALT EIL K
Sbjct: 241 AAKVVGRKWAPLPGVMATFLVSAIMHELIFYYLGRMKPNWELTWFFVIHGFALTVEILVK 300

Query: 301 KTLPAKWRLPAAVSGPLTVVFVFVTGIWLFFPQFVRCGIDVKAFAEYAAVGDFVRR 337
           K LP K RLPAAVS P+T+VF+F T IWLFFPQF+R  IDVKAF EYAA+  F+++
Sbjct: 301 KVLPEKRRLPAAVSVPVTLVFIFSTAIWLFFPQFMRLKIDVKAFEEYAALVSFIKK 344

[Close]

BLAST of Lag0031124 vs. NCBI nr
Match: XP_022982638.1 (acyl-CoA--sterol O-acyltransferase 1-like [Cucurbita maxima])

HSP 1 Score: 354.4 bits (908), Expect = 1.2e-93
Identity = 199/356 (55.90%), Postives = 238/356 (66.85%), Query Frame = 0


Query: 1   MEGEIMNFIQVWLTVSASLCYCHLIGNHVRPGRRRLISILPVVCLFLYLPLRLSSAHLGG 60
           MEGEI+NF  VWL V  SLCYCH  G  + PG  R ++I PVV LF  LPL+L+S HLGG
Sbjct: 1   MEGEIINFTMVWLIVCMSLCYCHTAGKILHPGAGRFLAISPVVLLFFLLPLQLTSVHLGG 60

Query: 61  ATAFFVAWLANFKLLLFSFNQGPLSSHPPLPLRRFLAASCLPIKILQNPSRNSQNGEYPS 120
           ATAFF+AWLANFKLLLF+   GPLS+ PPLPLRRFLA SCLPIKILQNPS      E  S
Sbjct: 61  ATAFFIAWLANFKLLLFAAGNGPLSATPPLPLRRFLALSCLPIKILQNPS------EKQS 120

Query: 121 PKSGPNRRQIQNQEHPIQKSFLSYAIKALFVAFM-----FARLLSPRTDP---------R 180
           PK   N  +  NQE    KSFL+Y IKAL VA M     ++  + P+             
Sbjct: 121 PK---NHHETLNQE---PKSFLNYWIKALIVALMVKLYDYSEFIHPKVILFIYSWHVYFF 180

Query: 181 RREIPRPESHRRRARAAVQRSVFLH---FASGL---LGRRWNLMVTGILNPTVYKPTLRL 240
              I        R    ++     H   F++ L    GRRWNLM T IL PTVYKPT+++
Sbjct: 181 LEIILAITQFFGRTLLGIELEPQFHDPYFSTSLQDFWGRRWNLMATNILQPTVYKPTVKI 240

Query: 241 VSPVVGRKWAPLPAVLATFGVSALMHELIFFYLGRKRPDWGISMFFVVHGVALTAEILAK 300
            + VVGRKWAPLP V+ATF VSA+MHELIF+YLGR +P+W ++ FFV+HG ALT EIL K
Sbjct: 241 AAKVVGRKWAPLPGVMATFLVSAIMHELIFYYLGRMKPNWELTWFFVIHGFALTVEILVK 300

Query: 301 KTLPAKWRLPAAVSGPLTVVFVFVTGIWLFFPQFVRCGIDVKAFAEYAAVGDFVRR 337
           K LP K RLPAAVS P+T+VF+F T IWLFFPQF+R  IDVKAF EYAA+  F+++
Sbjct: 301 KVLPEKRRLPAAVSVPVTLVFIFSTAIWLFFPQFMRLKIDVKAFEEYAALVSFMKK 344

[Close]

BLAST of Lag0031124 vs. NCBI nr
Match: XP_023527814.1 (acyl-CoA--sterol O-acyltransferase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 354.4 bits (908), Expect = 1.2e-93
Identity = 198/356 (55.62%), Postives = 238/356 (66.85%), Query Frame = 0


Query: 1   MEGEIMNFIQVWLTVSASLCYCHLIGNHVRPGRRRLISILPVVCLFLYLPLRLSSAHLGG 60
           MEGEI+NF  VWL V  SLCYCH  G  + PG  R ++I PVV LF  LPL+L+S HLGG
Sbjct: 1   MEGEIINFTMVWLIVCMSLCYCHTAGKILPPGAGRFLAIAPVVLLFFLLPLQLTSVHLGG 60

Query: 61  ATAFFVAWLANFKLLLFSFNQGPLSSHPPLPLRRFLAASCLPIKILQNPSRNSQNGEYPS 120
           ATAFF+AWLANFKLLLF+   GPLS+ PPLPLRRFLA SCLPIKILQNPS      E  S
Sbjct: 61  ATAFFIAWLANFKLLLFAAGNGPLSATPPLPLRRFLALSCLPIKILQNPS------EKQS 120

Query: 121 PKSGPNRRQIQNQEHPIQKSFLSYAIKALFVAFM-----FARLLSPRTDP---------R 180
           PK   N  +  NQE    KSFL+Y IKAL VA M     ++  + P+             
Sbjct: 121 PK---NHHETLNQE---PKSFLNYWIKALIVALMVKLYDYSEFIHPKVILFIYSWHVYFF 180

Query: 181 RREIPRPESHRRRARAAVQRSVFLH---FASGL---LGRRWNLMVTGILNPTVYKPTLRL 240
              I        R    ++     H   F++ L    GRRWNLM T IL PT+YKPT+++
Sbjct: 181 LEIILAITQFFGRTLLGIELEPQFHDPYFSTSLQDFWGRRWNLMATNILQPTIYKPTVKI 240

Query: 241 VSPVVGRKWAPLPAVLATFGVSALMHELIFFYLGRKRPDWGISMFFVVHGVALTAEILAK 300
            + VVGRKWAPLP V+ATF VSA+MHELIF+YLGR +P+W ++ FFV+HG ALT EIL K
Sbjct: 241 AAKVVGRKWAPLPGVMATFLVSAIMHELIFYYLGRMKPNWELTWFFVIHGFALTVEILVK 300

Query: 301 KTLPAKWRLPAAVSGPLTVVFVFVTGIWLFFPQFVRCGIDVKAFAEYAAVGDFVRR 337
           K LP K RLPAAVS P+T+VF+F T IWLFFPQF+R  IDVKAF EYAA+  F+++
Sbjct: 301 KVLPEKRRLPAAVSVPVTLVFIFSTAIWLFFPQFMRLKIDVKAFEEYAALVSFIKK 344

[Close]

BLAST of Lag0031124 vs. ExPASy Swiss-Prot
Match: Q9SV07 (Acyl-CoA--sterol O-acyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=ASAT1 PE=1 SV=1)

HSP 1 Score: 241.5 bits (615), Expect = 1.4e-62
Identity = 151/348 (43.39%), Postives = 193/348 (55.46%), Query Frame = 0


Query: 7   NFIQVWLTVSASLCYCHLIGNHVRPGRRRLISILPVVCLFLYLPLRLSSAHLGGATAFFV 66
           +FI+ W  V  SLCY   I   V  G +RLI   PV  +F  +P  + S HL G TAFF+
Sbjct: 3   SFIKAWGLVIISLCYTFFIAKLVPKGIKRLILFFPVFLIFFIVPFLIYSLHLLGITAFFI 62

Query: 67  AWLANFKLLLFSFNQGPLSS-HPPLPLRRFLAASCLPIKILQNPSRNSQNGEYPSPKSGP 126
           AWLANFKLLLF+  +GPLSS H PL L  FLA SCLPIKI  +P + ++   +     GP
Sbjct: 63  AWLANFKLLLFALGRGPLSSNHKPLSLPIFLAVSCLPIKIQLSP-KPTKTHSHEGSTEGP 122

Query: 127 NRRQIQNQEHPIQKSFLSYAIKALFVAFMFARLLSPRTDPRRREIPRPESH-------RR 186
                           L Y IKA+FV  +          P +  +     H         
Sbjct: 123 ----------------LIYTIKAVFVVLIIKAYEYSTKLPEKVVLTLYAIHIYFALEIIL 182

Query: 187 RARAAVQRSVF----------LHFASGL---LGRRWNLMVTGILNPTVYKPTLRLVSPVV 246
            A AA  R++            + A+ L    GRRWNLMVTGIL PTVY+P+L+L S V+
Sbjct: 183 AATAAAVRAMSDLELEPQFNKPYLATSLQDFWGRRWNLMVTGILRPTVYEPSLQLFS-VL 242

Query: 247 GRKWAPLPAVLATFGVSALMHELIFFYLGRKRPDWGISMFFVVHGVALTAEILAKKTLPA 306
           G  ++ + A   TF VS +MHELIFFY+GR RPDW +  FF+++G   T EI  KKT+  
Sbjct: 243 GPNYSQILAAFGTFVVSGIMHELIFFYMGRLRPDWKMMWFFLINGFCTTVEIAIKKTING 302

Query: 307 KWRLPAAVSGPLTVVFVFVTGIWLFFPQFVRCGIDVKAFAEYAAVGDF 334
           +WR P A+S  LT+ FV VT +WLF P+F RC I  KA  EYAA+G F
Sbjct: 303 RWRFPKAISQVLTLTFVMVTALWLFLPEFNRCNIVEKALDEYAAIGAF 332

[Close]

BLAST of Lag0031124 vs. ExPASy Swiss-Prot
Match: Q9XGY6 (Long-chain-alcohol O-fatty-acyltransferase OS=Simmondsia chinensis OX=3999 PE=1 SV=1)

HSP 1 Score: 218.4 bits (555), Expect = 1.3e-55
Identity = 142/360 (39.44%), Postives = 197/360 (54.72%), Query Frame = 0


Query: 1   MEGEIMNFIQVWLTVSASLCYCHLIGNHVRP--GRRRLISILPVVCLFLYLPLRLSSAHL 60
           +E E+  F +VW++  A+ CYC  +   V P  G  RL+ +LPVV LF++LPLRLSS HL
Sbjct: 3   VEKELKTFSEVWISAIAAACYCRFV-PAVAPHGGALRLLLLLPVVLLFIFLPLRLSSFHL 62

Query: 61  GGATAFFVAWLANFKLLLFSFNQGPLSSHPPLPLRRFLAASCLPIKILQNPSRNSQNGEY 120
           GG TA ++ WLANFKLLLF+F+ GPL S+P L L  F++ + LPIK   +PS + +    
Sbjct: 63  GGPTALYLVWLANFKLLLFAFHLGPL-SNPSLSLLHFISTTLLPIKFRDDPSNDHEK--- 122

Query: 121 PSPKSGPNRRQIQNQEHPIQKSFLSYAIKALFVAFMFARLLSPRTDPRR----------- 180
                  N+R +  +     +  + +  K +F A +  ++   R D              
Sbjct: 123 -------NKRTLSFE----WRKVVLFVAKLVFFAGIL-KIYEFRKDLPHFVISVLYCFHF 182

Query: 181 ---REIPRPESHRRRARAAVQRSVFLHFASGLL--------GRRWNLMVTGILNPTVYKP 240
               EI    S    ARA +   ++  F    L        GRRWNLMV+ IL  T Y+P
Sbjct: 183 YLGTEITLAAS-AVIARATLGLDLYPQFNEPYLATSLQDFWGRRWNLMVSDILGLTTYQP 242

Query: 241 TLRLVSPVVGRKWAPLPAVLATFGVSALMHELIFFYLGRKRPDWGISMFFVVHGVALTAE 300
             R++S  V  +W    A+L  F VS LMHE+ FFYL R RP W ++ FFV+HGV    E
Sbjct: 243 VRRVLSRWVRLRWEVAGAMLVAFTVSGLMHEVFFFYLTRARPSWEVTGFFVLHGVCTAVE 302

Query: 301 ILAKKTLPAKWRLPAAVSGPLTVVFVFVTGIWLFFPQFVRCGIDVKAFAEYAAVGDFVRR 337
           ++ KK +  K RL   VSG LTV FV VTG WLF PQ VR G+D+K   EY  + ++ ++
Sbjct: 303 MVVKKAVSGKVRLRREVSGALTVGFVMVTGGWLFLPQLVRHGVDLKTIDEYPVMFNYTQK 344

[Close]

BLAST of Lag0031124 vs. ExPASy Swiss-Prot
Match: Q9FJ76 (Probable long-chain-alcohol O-fatty-acyltransferase 5 OS=Arabidopsis thaliana OX=3702 GN=AT5 PE=2 SV=1)

HSP 1 Score: 200.3 bits (508), Expect = 3.6e-50
Identity = 130/348 (37.36%), Postives = 185/348 (53.16%), Query Frame = 0


Query: 1   MEGEIMNFIQVWLTVSASLCYCHLIGNHVRPGRRRLISILPVVCLFLYLPLRLSSAHLGG 60
           M+ E+ N I+VW++   S+ YC+ I   ++ G  R +S+ PV+ LFL LPL  SS HL  
Sbjct: 1   MDEELKNLIKVWVSAIISISYCYYIPPRIKSGAPRFLSVSPVLALFLVLPLFFSSLHLSL 60

Query: 61  ATAFFVAWLANFKLLLFSFNQGPLSSHPPLPLR--RFLAASCLPIKILQNPSRNSQNGEY 120
            TAFF+ WLANFKL+LFSF++GPL    P+P    RF   +C PIK+ QNP   SQN   
Sbjct: 61  ITAFFLTWLANFKLILFSFDKGPLI---PIPTNFPRFFCFTCFPIKVQQNP--KSQN-HL 120

Query: 121 PSPKSGPNRR--QIQNQEHPIQKSFLSYAIKALFVAFMFARLLSPRTDPRRREI------ 180
           P            +    +  +++     +  L+   ++  +    T  +          
Sbjct: 121 PKLVFAIKLAIFAVLLHLYSYRQNLSPTILLGLYFVHLYLEIEIILTFVKVVVFISLGCD 180

Query: 181 PRPESHRRRARAAVQRSVFLHFASGLLGRRWNLMVTGILNPTVYKPTLRLVSPVVGRKWA 240
             P+S++     ++Q            GRRWNLMV  IL P VY P  R+    +   WA
Sbjct: 181 LEPQSNKPYLATSLQ---------DFWGRRWNLMVPAILRPAVYAPMRRVSERKMSSGWA 240

Query: 241 PLPAVLATFGVSALMHELIFFYLGRKRPDWGISMFFVVHGVALTAEILAKK--TLPAKWR 300
             P +LA F VS L+HEL+FFYL R+ P   +++FFV+HGV    E+  KK  T+  +WR
Sbjct: 241 LFPGILAAFIVSGLVHELLFFYLIREMPTGEVTLFFVLHGVCTAVELAVKKKTTVAQRWR 300

Query: 301 LPAAVSGPLTVVFVFVTGIWLFFPQFVRCGIDVKAFAEYAAVGDFVRR 337
           L   VS  LTV FVFVTG WLF PQ  R G+  +  +E   + +F++R
Sbjct: 301 LSPGVSRVLTVGFVFVTGGWLFTPQLKRSGVMERFTSEAVLLVEFIKR 333

[Close]

BLAST of Lag0031124 vs. ExPASy Swiss-Prot
Match: Q9FJ72 (Probable long-chain-alcohol O-fatty-acyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=AT1 PE=2 SV=1)

HSP 1 Score: 194.9 bits (494), Expect = 1.5e-48
Identity = 130/359 (36.21%), Postives = 178/359 (49.58%), Query Frame = 0


Query: 1   MEGEIMNFIQVWLTVSASLCYCHLIGNHVRPGRRRLISILPVVCLFLYLPLRLSSAHLGG 60
           ME +  N I+VW++   SL YC+ I + +  G  RL+SILPV  LFL LPL LS  H   
Sbjct: 1   MEEKFRNLIEVWISALISLSYCYYISSKLSKGVLRLLSILPVCILFLVLPLFLSCVHFCA 60

Query: 61  ATAFFVAWLANFKLLLFSFNQGPLSSHPPLPLRRFLAASCLPIKILQNPSRNSQNGEYPS 120
            +  F++WLANFKLLLF+F++GPL   PP  L RF+  +CLPIKI Q+PS N+    +P 
Sbjct: 61  ISVLFLSWLANFKLLLFAFDEGPLFPLPP-KLSRFICFACLPIKIRQDPSPNAIPNLHPK 120

Query: 121 P----------------------KSGPNRRQIQNQEHPIQKSFLSYAIKALFVAFMFARL 180
           P                      + G  R  +          +L   +  +FV  + + L
Sbjct: 121 PMPKWVLAVKILVLGVLLHVYEYRDGLPRFVVLALY--CLHIYLEVELVLVFVGAVVSTL 180

Query: 181 LSPRTDPRRREIPRPESHRRRARAAVQRSVFLHFASGLLGRRWNLMVTGILNPTVYKPTL 240
           L    +P   E     S                       RRWNLMV+ +L  TV+ P  
Sbjct: 181 LGCNIEPVFNEPYLATS-----------------LQDFWSRRWNLMVSAVLRSTVHIPVQ 240

Query: 241 RLVSPVVGRKWAPLPAVLATFGVSALMHELIFFYLGRKRPDWGISMFFVVHGVALTAEIL 300
           R    ++    A    V+A+F VS LMHEL++FY+ RK P W ++ FFV+HG A   EI 
Sbjct: 241 RFFKRILSPDGAMFAGVMASFFVSGLMHELLYFYMIRKPPTWEVTCFFVLHGAATATEIA 300

Query: 301 AKKTLPAKWRLP--AAVSGPLTVVFVFVTGIWLFFPQFVRCGIDVKAFAEYAAVGDFVR 336
            K+T   +W  P   AVSG + + FV VTG+WLF  Q +R  +  KA  E   V D  +
Sbjct: 301 VKRT---QWLRPPHRAVSGLVVLTFVSVTGVWLFLAQVLRNNVHEKAIGECLLVLDLAK 336

[Close]

BLAST of Lag0031124 vs. ExPASy Swiss-Prot
Match: Q9FJ74 (Probable long-chain-alcohol O-fatty-acyltransferase 3 OS=Arabidopsis thaliana OX=3702 GN=AT3 PE=3 SV=1)

HSP 1 Score: 194.9 bits (494), Expect = 1.5e-48
Identity = 142/364 (39.01%), Postives = 192/364 (52.75%), Query Frame = 0


Query: 1   MEGEIMNFIQVWLTVSASLCYCHLIGNHVRPGRRRLISILPVVCLFLYLPLRLSSAHLGG 60
           ME E+ NFI++W++   S+ YC+ +   ++ G  RL+S+LPV  LFL  PL  S  H  G
Sbjct: 1   MEEELKNFIKLWISAIISISYCYYLSTGIKAGVFRLLSVLPVCALFLVFPLFFSYVHFSG 60

Query: 61  ATAFFVAWLANFKLLLFSFNQGPLSSHPPLP--LRRFLAASCLPIKILQNPSRNSQNGEY 120
             AFF++WLANFKL+LFSF+QGPLS   PLP  L RF+  +C PIK  QNP  N QN + 
Sbjct: 61  CMAFFLSWLANFKLILFSFDQGPLS---PLPRTLSRFICITCFPIKPQQNP--NIQNYKI 120

Query: 121 P----SPKSGPNRRQIQNQEHPIQKSFLSYAIKALF----------VAFMFARLLSPRTD 180
           P    + K       +Q  E+   K +LS A+  +F          + FM  + L   T 
Sbjct: 121 PIWLFAIKVVIFVVLLQMYEY---KQYLSPALLLVFNSLHIFLELEIVFMLVKALVFITL 180

Query: 181 PRRREIPRPESHRRRARAAVQRSVFLHFASGLLGRRWNLMVTGILNPTVYKPTLRLVSPV 240
               E   P+S+      ++Q            GRRWNLMV  IL P VY P  R+    
Sbjct: 181 GCDLE---PQSNEPYLATSLQ---------DFWGRRWNLMVPAILRPAVYLPARRMACRK 240

Query: 241 VGRKWAPLPAVLATFGVSALMHELIFFYLGRKRPDWGISMFFVVHGVALTAEILAKKTLP 300
           V    A    V A F VS  +HE++FFYL R+ P   ++ FF++HGV   AE+  KK+  
Sbjct: 241 VNSDQAMFLGVFAAFLVSGAVHEMLFFYLTREVPTGEVTWFFLLHGVCTVAEVAVKKSTF 300

Query: 301 AK--WRLPAAVSGPLTVVFVFVTGIWLFFPQFVRCGIDVKAFAEYAAVGDFVRRCVVLIG 347
            +  WR+   VS  LTV FV VT  W FFP  +R GI  +  +E     DFV+   +L+ 
Sbjct: 301 VRRWWRVSPTVSRLLTVGFVVVTSGWFFFP-LIRSGIIERLASEALMCIDFVKHKFLLL- 341

[Close]

BLAST of Lag0031124 vs. ExPASy TrEMBL
Match: A0A6J1DGQ4 (acyl-CoA--sterol O-acyltransferase 1-like OS=Momordica charantia OX=3673 GN=LOC111020333 PE=3 SV=1)

HSP 1 Score: 429.9 bits (1104), Expect = 1.0e-116
Identity = 242/367 (65.94%), Postives = 268/367 (73.02%), Query Frame = 0


Query: 1   MEGEIMNFIQVWLTVSASLCYCHLIGNHVRPGRRRLISILPVVCLFLYLPLRLSSAHLGG 60
           ME EI+NFI VWLTV ASLCYC+LIG +VRPG RRLISI PVVCLFLYLPLRLSSAHLGG
Sbjct: 1   MENEIINFINVWLTVCASLCYCYLIGKNVRPGGRRLISIFPVVCLFLYLPLRLSSAHLGG 60

Query: 61  ATAFFVAWLANFKLLLFSFNQGPLSSHPPLPLRRFLAASCLPIKILQNPSRNSQNGEYPS 120
            TAFF+AWLANFKLLLFSF  GPLSS PPLPLRRFLA SCLPIKI +N  + SQNGE  S
Sbjct: 61  PTAFFIAWLANFKLLLFSFAGGPLSSDPPLPLRRFLAVSCLPIKIQRNHLQISQNGENTS 120

Query: 121 PKSGPNRRQIQNQEHPI-QKSFLSYAIKALFVAFM-----FARLLSPRTDP--------- 180
           PKS PN  QI   E P+  KSFLSYAIK   VA M     ++  + P+            
Sbjct: 121 PKSSPNAPQI---ETPLPSKSFLSYAIKGFLVALMVKLYDYSEFMHPKLVMCIYSWHIYF 180

Query: 181 -RRREIPRPESHRRRARAAVQRSVF--LHFASGL---LGRRWNLMVTGILNPTVYKPTLR 240
                +   +   R    A     F   +F++ L    GRRWNLMVTGILNPTVYKPT+R
Sbjct: 181 LLEIILAAAKFLARNLLGAELEPQFNDPYFSTSLQDFWGRRWNLMVTGILNPTVYKPTIR 240

Query: 241 LVSPVVGRKWAPLPAVLATFGVSALMHELIFFYLGRKRPDWGISMFFVVHGVALTAEILA 300
           L +PVVGRKWAPL AVLATF VSA+MHELIF+YLGR RPDW +S FF VHGVALT EILA
Sbjct: 241 LAAPVVGRKWAPLLAVLATFFVSAMMHELIFYYLGRMRPDWEVSRFFAVHGVALTVEILA 300

Query: 301 KKTLPAKW-RLPAAVSGPLTVVFVFVTGIWLFFPQFVRCGIDVKAFAEYAAVGDFVRRCV 346
           KK  P K  R+PA VS PLTV FVFVTGIW+FFPQFVRCGIDVKAFAEYAAV DF  + +
Sbjct: 301 KKAWPEKCRRVPAVVSVPLTVGFVFVTGIWMFFPQFVRCGIDVKAFAEYAAVSDFFTKRL 360

[Close]

BLAST of Lag0031124 vs. ExPASy TrEMBL
Match: A0A6J1KAA2 (acyl-CoA--sterol O-acyltransferase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111493091 PE=3 SV=1)

HSP 1 Score: 354.8 bits (909), Expect = 4.3e-94
Identity = 190/360 (52.78%), Postives = 234/360 (65.00%), Query Frame = 0


Query: 1   MEGEIMNFIQVWLTVSASLCYCHLIGNHVRPGRRRLISILPVVCLFLYLPLRLSSAHLGG 60
           MEGEI+NFI+VWLTV  SLCYCH  G  + PG  R +++ P+V LF +LPL+L+S HLGG
Sbjct: 1   MEGEILNFIKVWLTVCISLCYCHATGKLIPPGAGRFLAVAPIVVLFFFLPLQLTSIHLGG 60

Query: 61  ATAFFVAWLANFKLLLFSFNQGPLSSHPPLPLRRFLAASCLPIKILQNPSRNSQNGEYPS 120
           +T+FF+AWLANFKLLLF+   GPLS+ PPLPLRRFLA SCLPIKILQN            
Sbjct: 61  STSFFIAWLANFKLLLFTAGDGPLSAEPPLPLRRFLALSCLPIKILQN------------ 120

Query: 121 PKSGPNRRQIQNQEHPIQKSFLSYAIKALFVAFM-----FARLLSPR------------- 180
            KS PN  +      P  KSFL+Y+IKA+ VA M     ++  + P+             
Sbjct: 121 EKSCPNNNRSDTTLKP--KSFLNYSIKAVIVALMVKLYDYSEFIHPKVVLFIYSWHLYFL 180

Query: 181 -------TDPRRREIPRPESHRRRARAAVQRSVFLHFASGLLGRRWNLMVTGILNPTVYK 240
                  T    R +   E          Q   F        GRRWNLM T IL PTVYK
Sbjct: 181 LEIILAITQFFGRNVLGIE-----LEPQFQDPYFSTSLQDFWGRRWNLMATNILQPTVYK 240

Query: 241 PTLRLVSPVVGRKWAPLPAVLATFGVSALMHELIFFYLGRKRPDWGISMFFVVHGVALTA 300
           P++++ + V+GRKWAPLPAV+ATF VSA+MHELIFFY+GR RP+W  + FFV+HGVALT 
Sbjct: 241 PSVKIAAKVIGRKWAPLPAVMATFLVSAMMHELIFFYMGRMRPNWECTWFFVIHGVALTV 300

Query: 301 EILAKKTLPAKWRLPAAVSGPLTVVFVFVTGIWLFFPQFVRCGIDVKAFAEYAAVGDFVR 336
           EI+ KK LP KWRLP AVS P+T+ F+F T IWLFFPQFVR  +DV+AF EYAA+  FV+
Sbjct: 301 EIMVKKVLPEKWRLPPAVSVPVTLAFIFPTAIWLFFPQFVRLKLDVRAFEEYAALVTFVK 341

[Close]

BLAST of Lag0031124 vs. ExPASy TrEMBL
Match: A0A6J1F6Y6 (acyl-CoA--sterol O-acyltransferase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111441427 PE=3 SV=1)

HSP 1 Score: 354.8 bits (909), Expect = 4.3e-94
Identity = 197/356 (55.34%), Postives = 239/356 (67.13%), Query Frame = 0


Query: 1   MEGEIMNFIQVWLTVSASLCYCHLIGNHVRPGRRRLISILPVVCLFLYLPLRLSSAHLGG 60
           MEGE++NFI VWL V  SLCYCH  G  + PG  R ++I PVV LF  LPL+L+S HLGG
Sbjct: 1   MEGEVINFIMVWLIVCMSLCYCHTAGKILPPGAGRFLAIAPVVVLFFLLPLQLTSVHLGG 60

Query: 61  ATAFFVAWLANFKLLLFSFNQGPLSSHPPLPLRRFLAASCLPIKILQNPSRNSQNGEYPS 120
           +TAFF+AWLANFKLLLF+   GPLS+ PPLPLRRFLA SCLPIKILQNPS      E  S
Sbjct: 61  STAFFIAWLANFKLLLFTAGNGPLSATPPLPLRRFLALSCLPIKILQNPS------EKQS 120

Query: 121 PKSGPNRRQIQNQEHPIQKSFLSYAIKALFVAFM-----FARLLSPRTDP---------R 180
           PK   N  +  NQE    KSFL+Y IKAL VA M     ++  + P+             
Sbjct: 121 PK---NHHETLNQE---PKSFLNYWIKALIVALMVKLYDYSEFIHPKVILFIYSWHVYFF 180

Query: 181 RREIPRPESHRRRARAAVQRSVFLH---FASGL---LGRRWNLMVTGILNPTVYKPTLRL 240
              I        R    ++     H   F++ L    GRRWNLM T IL PT+YKPT+++
Sbjct: 181 LEIILAITQFFGRTLLGIELEPQFHDPYFSTSLQDFWGRRWNLMATNILQPTIYKPTVKI 240

Query: 241 VSPVVGRKWAPLPAVLATFGVSALMHELIFFYLGRKRPDWGISMFFVVHGVALTAEILAK 300
            + VVGRKWAPLP V+ATF VSA+MHELIF+YLGR +P+W ++ FFV+HG ALT EIL K
Sbjct: 241 AAKVVGRKWAPLPGVMATFLVSAIMHELIFYYLGRMKPNWELTWFFVIHGFALTVEILVK 300

Query: 301 KTLPAKWRLPAAVSGPLTVVFVFVTGIWLFFPQFVRCGIDVKAFAEYAAVGDFVRR 337
           K LP K RLPAAVS P+T+VF+F T IWLFFPQF+R  IDVKAF EYAA+  F+++
Sbjct: 301 KVLPEKRRLPAAVSVPVTLVFIFSTAIWLFFPQFMRLKIDVKAFEEYAALVSFIKK 344

[Close]

BLAST of Lag0031124 vs. ExPASy TrEMBL
Match: A0A6J1J5C4 (acyl-CoA--sterol O-acyltransferase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111481453 PE=3 SV=1)

HSP 1 Score: 354.4 bits (908), Expect = 5.6e-94
Identity = 199/356 (55.90%), Postives = 238/356 (66.85%), Query Frame = 0


Query: 1   MEGEIMNFIQVWLTVSASLCYCHLIGNHVRPGRRRLISILPVVCLFLYLPLRLSSAHLGG 60
           MEGEI+NF  VWL V  SLCYCH  G  + PG  R ++I PVV LF  LPL+L+S HLGG
Sbjct: 1   MEGEIINFTMVWLIVCMSLCYCHTAGKILHPGAGRFLAISPVVLLFFLLPLQLTSVHLGG 60

Query: 61  ATAFFVAWLANFKLLLFSFNQGPLSSHPPLPLRRFLAASCLPIKILQNPSRNSQNGEYPS 120
           ATAFF+AWLANFKLLLF+   GPLS+ PPLPLRRFLA SCLPIKILQNPS      E  S
Sbjct: 61  ATAFFIAWLANFKLLLFAAGNGPLSATPPLPLRRFLALSCLPIKILQNPS------EKQS 120

Query: 121 PKSGPNRRQIQNQEHPIQKSFLSYAIKALFVAFM-----FARLLSPRTDP---------R 180
           PK   N  +  NQE    KSFL+Y IKAL VA M     ++  + P+             
Sbjct: 121 PK---NHHETLNQE---PKSFLNYWIKALIVALMVKLYDYSEFIHPKVILFIYSWHVYFF 180

Query: 181 RREIPRPESHRRRARAAVQRSVFLH---FASGL---LGRRWNLMVTGILNPTVYKPTLRL 240
              I        R    ++     H   F++ L    GRRWNLM T IL PTVYKPT+++
Sbjct: 181 LEIILAITQFFGRTLLGIELEPQFHDPYFSTSLQDFWGRRWNLMATNILQPTVYKPTVKI 240

Query: 241 VSPVVGRKWAPLPAVLATFGVSALMHELIFFYLGRKRPDWGISMFFVVHGVALTAEILAK 300
            + VVGRKWAPLP V+ATF VSA+MHELIF+YLGR +P+W ++ FFV+HG ALT EIL K
Sbjct: 241 AAKVVGRKWAPLPGVMATFLVSAIMHELIFYYLGRMKPNWELTWFFVIHGFALTVEILVK 300

Query: 301 KTLPAKWRLPAAVSGPLTVVFVFVTGIWLFFPQFVRCGIDVKAFAEYAAVGDFVRR 337
           K LP K RLPAAVS P+T+VF+F T IWLFFPQF+R  IDVKAF EYAA+  F+++
Sbjct: 301 KVLPEKRRLPAAVSVPVTLVFIFSTAIWLFFPQFMRLKIDVKAFEEYAALVSFMKK 344

[Close]

BLAST of Lag0031124 vs. ExPASy TrEMBL
Match: A0A6J1GBE5 (acyl-CoA--sterol O-acyltransferase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111452458 PE=3 SV=1)

HSP 1 Score: 351.3 bits (900), Expect = 4.7e-93
Identity = 189/360 (52.50%), Postives = 233/360 (64.72%), Query Frame = 0


Query: 1   MEGEIMNFIQVWLTVSASLCYCHLIGNHVRPGRRRLISILPVVCLFLYLPLRLSSAHLGG 60
           MEGEI+NFI+VWLTV  SLCYCH  G  + PG  R +++ P+V LF  LPL+L+S HLGG
Sbjct: 1   MEGEILNFIKVWLTVCISLCYCHATGKLLAPGPGRFLAVAPIVVLFFLLPLQLTSIHLGG 60

Query: 61  ATAFFVAWLANFKLLLFSFNQGPLSSHPPLPLRRFLAASCLPIKILQNPSRNSQNGEYPS 120
           +T+FF+AWLANFKLLLF+   GPLS+ PPLPLRRFLA SCLPIKILQN            
Sbjct: 61  STSFFIAWLANFKLLLFAAGDGPLSAEPPLPLRRFLALSCLPIKILQN------------ 120

Query: 121 PKSGPNRRQIQNQEHPIQKSFLSYAIKALFVAFM-----FARLLSPR------------- 180
            KS PN  +      P  KSFL+Y+IKA+ VA M     ++  + P+             
Sbjct: 121 EKSCPNNNRSDTTLKP--KSFLNYSIKAVIVALMVKLYDYSEFIHPKVVLFIYSWHLYFL 180

Query: 181 -------TDPRRREIPRPESHRRRARAAVQRSVFLHFASGLLGRRWNLMVTGILNPTVYK 240
                  T    R +   E          Q   F        GRRWNLM T IL PTVYK
Sbjct: 181 LEIILAITQFFGRNVLGIE-----LEPQFQDPYFSTSLQDFWGRRWNLMATNILQPTVYK 240

Query: 241 PTLRLVSPVVGRKWAPLPAVLATFGVSALMHELIFFYLGRKRPDWGISMFFVVHGVALTA 300
           P++++ + V+GRKWAPLPAV+ATF VSA+MHELIF+Y+GR RP+W  + FFV+HGVALT 
Sbjct: 241 PSVKIAAKVIGRKWAPLPAVMATFLVSAMMHELIFYYMGRMRPNWECTWFFVIHGVALTV 300

Query: 301 EILAKKTLPAKWRLPAAVSGPLTVVFVFVTGIWLFFPQFVRCGIDVKAFAEYAAVGDFVR 336
           EI+ KK LP KWRLP AVS P+T+ F+F T IWLFFPQFVR  +DV+AF EYAA+  FV+
Sbjct: 301 EIMVKKVLPEKWRLPPAVSVPVTLAFIFPTAIWLFFPQFVRLKLDVRAFEEYAALVTFVK 341

[Close]

BLAST of Lag0031124 vs. TAIR 10
Match: AT3G51970.1 (acyl-CoA sterol acyl transferase 1 )

HSP 1 Score: 241.5 bits (615), Expect = 1.0e-63
Identity = 151/348 (43.39%), Postives = 193/348 (55.46%), Query Frame = 0


Query: 7   NFIQVWLTVSASLCYCHLIGNHVRPGRRRLISILPVVCLFLYLPLRLSSAHLGGATAFFV 66
           +FI+ W  V  SLCY   I   V  G +RLI   PV  +F  +P  + S HL G TAFF+
Sbjct: 3   SFIKAWGLVIISLCYTFFIAKLVPKGIKRLILFFPVFLIFFIVPFLIYSLHLLGITAFFI 62

Query: 67  AWLANFKLLLFSFNQGPLSS-HPPLPLRRFLAASCLPIKILQNPSRNSQNGEYPSPKSGP 126
           AWLANFKLLLF+  +GPLSS H PL L  FLA SCLPIKI  +P + ++   +     GP
Sbjct: 63  AWLANFKLLLFALGRGPLSSNHKPLSLPIFLAVSCLPIKIQLSP-KPTKTHSHEGSTEGP 122

Query: 127 NRRQIQNQEHPIQKSFLSYAIKALFVAFMFARLLSPRTDPRRREIPRPESH-------RR 186
                           L Y IKA+FV  +          P +  +     H         
Sbjct: 123 ----------------LIYTIKAVFVVLIIKAYEYSTKLPEKVVLTLYAIHIYFALEIIL 182

Query: 187 RARAAVQRSVF----------LHFASGL---LGRRWNLMVTGILNPTVYKPTLRLVSPVV 246
            A AA  R++            + A+ L    GRRWNLMVTGIL PTVY+P+L+L S V+
Sbjct: 183 AATAAAVRAMSDLELEPQFNKPYLATSLQDFWGRRWNLMVTGILRPTVYEPSLQLFS-VL 242

Query: 247 GRKWAPLPAVLATFGVSALMHELIFFYLGRKRPDWGISMFFVVHGVALTAEILAKKTLPA 306
           G  ++ + A   TF VS +MHELIFFY+GR RPDW +  FF+++G   T EI  KKT+  
Sbjct: 243 GPNYSQILAAFGTFVVSGIMHELIFFYMGRLRPDWKMMWFFLINGFCTTVEIAIKKTING 302

Query: 307 KWRLPAAVSGPLTVVFVFVTGIWLFFPQFVRCGIDVKAFAEYAAVGDF 334
           +WR P A+S  LT+ FV VT +WLF P+F RC I  KA  EYAA+G F
Sbjct: 303 RWRFPKAISQVLTLTFVMVTALWLFLPEFNRCNIVEKALDEYAAIGAF 332

[Close]

BLAST of Lag0031124 vs. TAIR 10
Match: AT5G55340.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 200.3 bits (508), Expect = 2.6e-51
Identity = 130/348 (37.36%), Postives = 185/348 (53.16%), Query Frame = 0


Query: 1   MEGEIMNFIQVWLTVSASLCYCHLIGNHVRPGRRRLISILPVVCLFLYLPLRLSSAHLGG 60
           M+ E+ N I+VW++   S+ YC+ I   ++ G  R +S+ PV+ LFL LPL  SS HL  
Sbjct: 1   MDEELKNLIKVWVSAIISISYCYYIPPRIKSGAPRFLSVSPVLALFLVLPLFFSSLHLSL 60

Query: 61  ATAFFVAWLANFKLLLFSFNQGPLSSHPPLPLR--RFLAASCLPIKILQNPSRNSQNGEY 120
            TAFF+ WLANFKL+LFSF++GPL    P+P    RF   +C PIK+ QNP   SQN   
Sbjct: 61  ITAFFLTWLANFKLILFSFDKGPLI---PIPTNFPRFFCFTCFPIKVQQNP--KSQN-HL 120

Query: 121 PSPKSGPNRR--QIQNQEHPIQKSFLSYAIKALFVAFMFARLLSPRTDPRRREI------ 180
           P            +    +  +++     +  L+   ++  +    T  +          
Sbjct: 121 PKLVFAIKLAIFAVLLHLYSYRQNLSPTILLGLYFVHLYLEIEIILTFVKVVVFISLGCD 180

Query: 181 PRPESHRRRARAAVQRSVFLHFASGLLGRRWNLMVTGILNPTVYKPTLRLVSPVVGRKWA 240
             P+S++     ++Q            GRRWNLMV  IL P VY P  R+    +   WA
Sbjct: 181 LEPQSNKPYLATSLQ---------DFWGRRWNLMVPAILRPAVYAPMRRVSERKMSSGWA 240

Query: 241 PLPAVLATFGVSALMHELIFFYLGRKRPDWGISMFFVVHGVALTAEILAKK--TLPAKWR 300
             P +LA F VS L+HEL+FFYL R+ P   +++FFV+HGV    E+  KK  T+  +WR
Sbjct: 241 LFPGILAAFIVSGLVHELLFFYLIREMPTGEVTLFFVLHGVCTAVELAVKKKTTVAQRWR 300

Query: 301 LPAAVSGPLTVVFVFVTGIWLFFPQFVRCGIDVKAFAEYAAVGDFVRR 337
           L   VS  LTV FVFVTG WLF PQ  R G+  +  +E   + +F++R
Sbjct: 301 LSPGVSRVLTVGFVFVTGGWLFTPQLKRSGVMERFTSEAVLLVEFIKR 333

[Close]

BLAST of Lag0031124 vs. TAIR 10
Match: AT5G55380.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 194.9 bits (494), Expect = 1.1e-49
Identity = 130/359 (36.21%), Postives = 178/359 (49.58%), Query Frame = 0


Query: 1   MEGEIMNFIQVWLTVSASLCYCHLIGNHVRPGRRRLISILPVVCLFLYLPLRLSSAHLGG 60
           ME +  N I+VW++   SL YC+ I + +  G  RL+SILPV  LFL LPL LS  H   
Sbjct: 1   MEEKFRNLIEVWISALISLSYCYYISSKLSKGVLRLLSILPVCILFLVLPLFLSCVHFCA 60

Query: 61  ATAFFVAWLANFKLLLFSFNQGPLSSHPPLPLRRFLAASCLPIKILQNPSRNSQNGEYPS 120
            +  F++WLANFKLLLF+F++GPL   PP  L RF+  +CLPIKI Q+PS N+    +P 
Sbjct: 61  ISVLFLSWLANFKLLLFAFDEGPLFPLPP-KLSRFICFACLPIKIRQDPSPNAIPNLHPK 120

Query: 121 P----------------------KSGPNRRQIQNQEHPIQKSFLSYAIKALFVAFMFARL 180
           P                      + G  R  +          +L   +  +FV  + + L
Sbjct: 121 PMPKWVLAVKILVLGVLLHVYEYRDGLPRFVVLALY--CLHIYLEVELVLVFVGAVVSTL 180

Query: 181 LSPRTDPRRREIPRPESHRRRARAAVQRSVFLHFASGLLGRRWNLMVTGILNPTVYKPTL 240
           L    +P   E     S                       RRWNLMV+ +L  TV+ P  
Sbjct: 181 LGCNIEPVFNEPYLATS-----------------LQDFWSRRWNLMVSAVLRSTVHIPVQ 240

Query: 241 RLVSPVVGRKWAPLPAVLATFGVSALMHELIFFYLGRKRPDWGISMFFVVHGVALTAEIL 300
           R    ++    A    V+A+F VS LMHEL++FY+ RK P W ++ FFV+HG A   EI 
Sbjct: 241 RFFKRILSPDGAMFAGVMASFFVSGLMHELLYFYMIRKPPTWEVTCFFVLHGAATATEIA 300

Query: 301 AKKTLPAKWRLP--AAVSGPLTVVFVFVTGIWLFFPQFVRCGIDVKAFAEYAAVGDFVR 336
            K+T   +W  P   AVSG + + FV VTG+WLF  Q +R  +  KA  E   V D  +
Sbjct: 301 VKRT---QWLRPPHRAVSGLVVLTFVSVTGVWLFLAQVLRNNVHEKAIGECLLVLDLAK 336

[Close]

BLAST of Lag0031124 vs. TAIR 10
Match: AT5G55360.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 194.9 bits (494), Expect = 1.1e-49
Identity = 142/364 (39.01%), Postives = 192/364 (52.75%), Query Frame = 0


Query: 1   MEGEIMNFIQVWLTVSASLCYCHLIGNHVRPGRRRLISILPVVCLFLYLPLRLSSAHLGG 60
           ME E+ NFI++W++   S+ YC+ +   ++ G  RL+S+LPV  LFL  PL  S  H  G
Sbjct: 1   MEEELKNFIKLWISAIISISYCYYLSTGIKAGVFRLLSVLPVCALFLVFPLFFSYVHFSG 60

Query: 61  ATAFFVAWLANFKLLLFSFNQGPLSSHPPLP--LRRFLAASCLPIKILQNPSRNSQNGEY 120
             AFF++WLANFKL+LFSF+QGPLS   PLP  L RF+  +C PIK  QNP  N QN + 
Sbjct: 61  CMAFFLSWLANFKLILFSFDQGPLS---PLPRTLSRFICITCFPIKPQQNP--NIQNYKI 120

Query: 121 P----SPKSGPNRRQIQNQEHPIQKSFLSYAIKALF----------VAFMFARLLSPRTD 180
           P    + K       +Q  E+   K +LS A+  +F          + FM  + L   T 
Sbjct: 121 PIWLFAIKVVIFVVLLQMYEY---KQYLSPALLLVFNSLHIFLELEIVFMLVKALVFITL 180

Query: 181 PRRREIPRPESHRRRARAAVQRSVFLHFASGLLGRRWNLMVTGILNPTVYKPTLRLVSPV 240
               E   P+S+      ++Q            GRRWNLMV  IL P VY P  R+    
Sbjct: 181 GCDLE---PQSNEPYLATSLQ---------DFWGRRWNLMVPAILRPAVYLPARRMACRK 240

Query: 241 VGRKWAPLPAVLATFGVSALMHELIFFYLGRKRPDWGISMFFVVHGVALTAEILAKKTLP 300
           V    A    V A F VS  +HE++FFYL R+ P   ++ FF++HGV   AE+  KK+  
Sbjct: 241 VNSDQAMFLGVFAAFLVSGAVHEMLFFYLTREVPTGEVTWFFLLHGVCTVAEVAVKKSTF 300

Query: 301 AK--WRLPAAVSGPLTVVFVFVTGIWLFFPQFVRCGIDVKAFAEYAAVGDFVRRCVVLIG 347
            +  WR+   VS  LTV FV VT  W FFP  +R GI  +  +E     DFV+   +L+ 
Sbjct: 301 VRRWWRVSPTVSRLLTVGFVVVTSGWFFFP-LIRSGIIERLASEALMCIDFVKHKFLLL- 341

[Close]

BLAST of Lag0031124 vs. TAIR 10
Match: AT5G55350.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 194.1 bits (492), Expect = 1.9e-49
Identity = 125/327 (38.23%), Postives = 176/327 (53.82%), Query Frame = 0


Query: 1   MEGEIMNFIQVWLTVSASLCYCHLIGNHVRPGRRRLISILPVVCLFLYLPLRLSSAHLGG 60
           ME E+M+ I+VW+    S+ YC+     ++ G  RL+S+LPV  LFL LPL +SS H  G
Sbjct: 1   MEEELMSLIKVWVYAIISISYCYYTSTRIKSGVFRLLSVLPVCVLFLVLPLFVSSVHFSG 60

Query: 61  ATAFFVAWLANFKLLLFSFNQGPLSSHPPLPLRRFLAASCLPIKILQNPSRNSQNGEYPS 120
           +TAFF++WLANFKL+LFSF+QGPL    P  L RF+  +C PIK+ QNP   +Q  ++  
Sbjct: 61  STAFFLSWLANFKLILFSFDQGPLFP-VPSNLSRFVCFTCFPIKLQQNPKPQNQMPKWGF 120

Query: 121 PKS----GPNRRQIQNQEHPIQKSFLSYAIKALFVAFMFARLLSPRTDPRRREI---PRP 180
                  G      + ++H      L   + +L +   +  LL+P        +     P
Sbjct: 121 AVKLAFFGVLLHMYEYKQHMSPTVLL--VLYSLHIYLEYEILLAPLKVLLSISLWCDLEP 180

Query: 181 ESHRRRARAAVQRSVFLHFASGLLGRRWNLMVTGILNPTVYKPTLRLVSPVVGRKWAPLP 240
             +      ++Q            GRRWNLMV  IL P VY P  ++    +    A   
Sbjct: 181 HFNEPYLSTSLQ---------DFWGRRWNLMVPAILRPAVYLPVRQMAGRKMNSDQALFL 240

Query: 241 AVLATFGVSALMHELIFFYLGRKRPDWGISMFFVVHGVALTAEILAKKT-LPAKWRLPAA 300
            V A+F VS ++HELIFFY  R+ P   +++FFV+HGV   AE  AK+T L  +W++   
Sbjct: 241 GVFASFLVSGVVHELIFFYFTRESPTGEVTLFFVLHGVCTAAECAAKRTRLVRRWKVSQM 300

Query: 301 VSGPLTVVFVFVTGIWLFFPQFVRCGI 320
           VS  LTV FV +TG WLFFP   R G+
Sbjct: 301 VSRLLTVGFVVMTGGWLFFPHLARSGM 315

The following BLAST results are available for this feature:

BLAST of Lag0031124 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022152669.1	2.2e-116	65.94	acyl-CoA--sterol O-acyltransferase 1-like [Momordica charantia]	[more]
XP_022998466.1	8.8e-94	52.78	acyl-CoA--sterol O-acyltransferase 1-like [Cucurbita maxima]	[more]
XP_022934183.1	8.8e-94	55.34	acyl-CoA--sterol O-acyltransferase 1-like [Cucurbita moschata]	[more]
XP_022982638.1	1.2e-93	55.90	acyl-CoA--sterol O-acyltransferase 1-like [Cucurbita maxima]	[more]
XP_023527814.1	1.2e-93	55.62	acyl-CoA--sterol O-acyltransferase 1-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of Lag0031124 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SV07	1.4e-62	43.39	Acyl-CoA--sterol O-acyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=ASAT1 PE...	[more]
Q9XGY6	1.3e-55	39.44	Long-chain-alcohol O-fatty-acyltransferase OS=Simmondsia chinensis OX=3999 PE=1 ...	[more]
Q9FJ76	3.6e-50	37.36	Probable long-chain-alcohol O-fatty-acyltransferase 5 OS=Arabidopsis thaliana OX...	[more]
Q9FJ72	1.5e-48	36.21	Probable long-chain-alcohol O-fatty-acyltransferase 1 OS=Arabidopsis thaliana OX...	[more]
Q9FJ74	1.5e-48	39.01	Probable long-chain-alcohol O-fatty-acyltransferase 3 OS=Arabidopsis thaliana OX...	[more]

BLAST of Lag0031124 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DGQ4	1.0e-116	65.94	acyl-CoA--sterol O-acyltransferase 1-like OS=Momordica charantia OX=3673 GN=LOC1...	[more]
A0A6J1KAA2	4.3e-94	52.78	acyl-CoA--sterol O-acyltransferase 1-like OS=Cucurbita maxima OX=3661 GN=LOC1114...	[more]
A0A6J1F6Y6	4.3e-94	55.34	acyl-CoA--sterol O-acyltransferase 1-like OS=Cucurbita moschata OX=3662 GN=LOC11...	[more]
A0A6J1J5C4	5.6e-94	55.90	acyl-CoA--sterol O-acyltransferase 1-like OS=Cucurbita maxima OX=3661 GN=LOC1114...	[more]
A0A6J1GBE5	4.7e-93	52.50	acyl-CoA--sterol O-acyltransferase 1-like OS=Cucurbita moschata OX=3662 GN=LOC11...	[more]

BLAST of Lag0031124 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G51970.1	1.0e-63	43.39	acyl-CoA sterol acyl transferase 1	[more]
AT5G55340.1	2.6e-51	37.36	MBOAT (membrane bound O-acyl transferase) family protein	[more]
AT5G55380.1	1.1e-49	36.21	MBOAT (membrane bound O-acyl transferase) family protein	[more]
AT5G55360.1	1.1e-49	39.01	MBOAT (membrane bound O-acyl transferase) family protein	[more]
AT5G55350.1	1.9e-49	38.23	MBOAT (membrane bound O-acyl transferase) family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR032805	Wax synthase domain	PFAM	PF13813	MBOAT_2	coord: 198..267 e-value: 1.1E-7 score: 32.0
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 108..129
None	No IPR available	PANTHER	PTHR31595:SF56	ACYL-COA--STEROL O-ACYLTRANSFERASE 1-LIKE	coord: 197..336 coord: 2..155
IPR044851	Wax synthase	PANTHER	PTHR31595	LONG-CHAIN-ALCOHOL O-FATTY-ACYLTRANSFERASE 3-RELATED	coord: 197..336 coord: 2..155

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0031124.1	Lag0031124.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006629	lipid metabolic process
cellular_component	GO:0016020	membrane
molecular_function	GO:0008374	O-acyltransferase activity