Lag0031120 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0031120
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	acyl-CoA--sterol O-acyltransferase 1-like
Location	chr11: 5008824 .. 5009876 (-)
RNA-Seq Expression	Lag0031120
Synteny	Lag0031120

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCCGCCGATGAGATTCGTATCTTCCTCCAAGCTATGCTTCAAGTTTCATCTTCCCTCTGTTTCTGTTTCGCCATTAGAAACTCAATCCCAAATGGCTTCTTCAAATTCCTCTTAATCCTCCCTCTCTTCTTCCTCTTCCTCTACATTCCTCTCCAGTTTCACACCATCCATCTTCAGGGCGGCGTCGGCTTCTTCATCGGCTGGCTCGGCAGCTTCAAGCTCCTCCTCTTCGCCTTCGGCAAAGGCCCTCTCTGCTCCGCCGCCACCTCCTCCTCCCTCCGCCGCTTCCTCGCCGTTGGATCCCTCCCAATCGAAATCCCCGACGAAGAATCGCCGCCCAACCGCTCCGATCCTCTCACCTTCCCCGCTAAGTTAGGGTTCCTAATTCTCACCATCGCCGGGATCTATTTCAAAAATCAATTCCATCCAAACGCAGCCCTAGTTTTCTTCTGCCTCCTAATTTACCTCCTCCTCGAGATTCTCATCGGCAGCGCCGCCGCCCTGGCCAAGGCGGCGCTCGGCGTTGAACTATTGCCGTATTTCCACGAGCCGTATCTCTCTTCTTCGTTGCAGGACTTTTGGGGGCGGCGATGGAATCTAATGACATCCAGAATCCTCCGCCTCGCCGTCTACGATCCGTGCCGGAAACTAGCCGTCGCCGTCGTCGGGAGGAAACCGGCGGTGATATTGGCGGTGATGGCGACGTTCGCCGTCTCCGGTCTGATGCACGAACTGATCTACTTCTACATGGGGAGACTGAGGCCGACATGGGAGGTGACTTGCTTCTTCCTGCTACATGGAGTTTGTTTGGCGGCGGAGATGACGGTGAAGACGGCGGTCGGCGGGAGGTTTCGGCCTCCGTGGATTGTGACGGTGCCGATGACGATTGGGTTCATAATGGTGACGAGTTTTTGGCTGTTTTTTCCGCAGTTTTTAAGGTTTAAAGCGGACGTTAGAATGTTTGAAGAGCACGCGGCTTTGGGCGCGTTGGTTAAGAACGTGACGGGCGAGTGGATCACGCCCTTGAAATTCAGCTCCTTTCTAAGTTAA

mRNA sequence

ATGGCCGCCGATGAGATTCGTATCTTCCTCCAAGCTATGCTTCAAGTTTCATCTTCCCTCTGTTTCTGTTTCGCCATTAGAAACTCAATCCCAAATGGCTTCTTCAAATTCCTCTTAATCCTCCCTCTCTTCTTCCTCTTCCTCTACATTCCTCTCCAGTTTCACACCATCCATCTTCAGGGCGGCGTCGGCTTCTTCATCGGCTGGCTCGGCAGCTTCAAGCTCCTCCTCTTCGCCTTCGGCAAAGGCCCTCTCTGCTCCGCCGCCACCTCCTCCTCCCTCCGCCGCTTCCTCGCCGTTGGATCCCTCCCAATCGAAATCCCCGACGAAGAATCGCCGCCCAACCGCTCCGATCCTCTCACCTTCCCCGCTAAGTTAGGGTTCCTAATTCTCACCATCGCCGGGATCTATTTCAAAAATCAATTCCATCCAAACGCAGCCCTAGTTTTCTTCTGCCTCCTAATTTACCTCCTCCTCGAGATTCTCATCGGCAGCGCCGCCGCCCTGGCCAAGGCGGCGCTCGGCGTTGAACTATTGCCGTATTTCCACGAGCCGTATCTCTCTTCTTCGTTGCAGGACTTTTGGGGGCGGCGATGGAATCTAATGACATCCAGAATCCTCCGCCTCGCCGTCTACGATCCGTGCCGGAAACTAGCCGTCGCCGTCGTCGGGAGGAAACCGGCGGTGATATTGGCGGTGATGGCGACGTTCGCCGTCTCCGGTCTGATGCACGAACTGATCTACTTCTACATGGGGAGACTGAGGCCGACATGGGAGGTGACTTGCTTCTTCCTGCTACATGGAGTTTGTTTGGCGGCGGAGATGACGGTGAAGACGGCGGTCGGCGGGAGGTTTCGGCCTCCGTGGATTGTGACGGTGCCGATGACGATTGGGTTCATAATGGTGACGAGTTTTTGGCTGTTTTTTCCGCAGTTTTTAAGGTTTAAAGCGGACGTTAGAATGTTTGAAGAGCACGCGGCTTTGGGCGCGTTGGTTAAGAACGTGACGGGCGAGTGGATCACGCCCTTGAAATTCAGCTCCTTTCTAAGTTAA

Coding sequence (CDS)

ATGGCCGCCGATGAGATTCGTATCTTCCTCCAAGCTATGCTTCAAGTTTCATCTTCCCTCTGTTTCTGTTTCGCCATTAGAAACTCAATCCCAAATGGCTTCTTCAAATTCCTCTTAATCCTCCCTCTCTTCTTCCTCTTCCTCTACATTCCTCTCCAGTTTCACACCATCCATCTTCAGGGCGGCGTCGGCTTCTTCATCGGCTGGCTCGGCAGCTTCAAGCTCCTCCTCTTCGCCTTCGGCAAAGGCCCTCTCTGCTCCGCCGCCACCTCCTCCTCCCTCCGCCGCTTCCTCGCCGTTGGATCCCTCCCAATCGAAATCCCCGACGAAGAATCGCCGCCCAACCGCTCCGATCCTCTCACCTTCCCCGCTAAGTTAGGGTTCCTAATTCTCACCATCGCCGGGATCTATTTCAAAAATCAATTCCATCCAAACGCAGCCCTAGTTTTCTTCTGCCTCCTAATTTACCTCCTCCTCGAGATTCTCATCGGCAGCGCCGCCGCCCTGGCCAAGGCGGCGCTCGGCGTTGAACTATTGCCGTATTTCCACGAGCCGTATCTCTCTTCTTCGTTGCAGGACTTTTGGGGGCGGCGATGGAATCTAATGACATCCAGAATCCTCCGCCTCGCCGTCTACGATCCGTGCCGGAAACTAGCCGTCGCCGTCGTCGGGAGGAAACCGGCGGTGATATTGGCGGTGATGGCGACGTTCGCCGTCTCCGGTCTGATGCACGAACTGATCTACTTCTACATGGGGAGACTGAGGCCGACATGGGAGGTGACTTGCTTCTTCCTGCTACATGGAGTTTGTTTGGCGGCGGAGATGACGGTGAAGACGGCGGTCGGCGGGAGGTTTCGGCCTCCGTGGATTGTGACGGTGCCGATGACGATTGGGTTCATAATGGTGACGAGTTTTTGGCTGTTTTTTCCGCAGTTTTTAAGGTTTAAAGCGGACGTTAGAATGTTTGAAGAGCACGCGGCTTTGGGCGCGTTGGTTAAGAACGTGACGGGCGAGTGGATCACGCCCTTGAAATTCAGCTCCTTTCTAAGTTAA

Protein sequence

MAADEIRIFLQAMLQVSSSLCFCFAIRNSIPNGFFKFLLILPLFFLFLYIPLQFHTIHLQGGVGFFIGWLGSFKLLLFAFGKGPLCSAATSSSLRRFLAVGSLPIEIPDEESPPNRSDPLTFPAKLGFLILTIAGIYFKNQFHPNAALVFFCLLIYLLLEILIGSAAALAKAALGVELLPYFHEPYLSSSLQDFWGRRWNLMTSRILRLAVYDPCRKLAVAVVGRKPAVILAVMATFAVSGLMHELIYFYMGRLRPTWEVTCFFLLHGVCLAAEMTVKTAVGGRFRPPWIVTVPMTIGFIMVTSFWLFFPQFLRFKADVRMFEEHAALGALVKNVTGEWITPLKFSSFLS

Homology

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BLAST of Lag0031120 vs. NCBI nr
Match: XP_038905685.1 (acyl-CoA--sterol O-acyltransferase 1-like [Benincasa hispida])

HSP 1 Score: 544.3 bits (1401), Expect = 7.8e-151
Identity = 272/349 (77.94%), Postives = 298/349 (85.39%), Query Frame = 0


Query: 3   ADEIRIFLQAMLQVSSSLCFCFAIRNSIPNGFFKFLLILPLFFLFLYIPLQFHTIHLQGG 62
           A EIRIFLQ +  VSSSLCFCF IRNSIPNGF KFLLILP FFLFLYIPLQF +IH QG 
Sbjct: 2   AAEIRIFLQTLFLVSSSLCFCFTIRNSIPNGFLKFLLILPFFFLFLYIPLQFQSIHFQGS 61

Query: 63  VGFFIGWLGSFKLLLFAFGKGPLCSAATSSSLRRFLAVGSLPIEIPDEESPPNRSD---- 122
           +GFFIGWLGSFKLLLFAFGKGPLCSAA SSSLR FLAVGSLPIEIPD +   N S     
Sbjct: 62  IGFFIGWLGSFKLLLFAFGKGPLCSAAASSSLRHFLAVGSLPIEIPDHKPAANHSHSNPV 121

Query: 123 -PLTFPAKLGFLILTIAGIYFKNQFHPNAALVFFCLLIYLLLEILIGSAAALAKAALGVE 182
            PL+F +KLG LILTI  IYFKN FHPNA L+FFC +IYLLLEIL+G AAALAKA LGVE
Sbjct: 122 LPLSFSSKLGLLILTIVAIYFKNLFHPNAILIFFCFVIYLLLEILLGGAAALAKATLGVE 181

Query: 183 LLPYFHEPYLSSSLQDFWGRRWNLMTSRILRLAVYDPCRKLAVAVVGRKPAVILAVMATF 242
           LLPYF EPYLS SLQDFWGRRWNLMTSRILRL VYDPCRK+ + +VGRKPA ++A+ ATF
Sbjct: 182 LLPYFDEPYLSDSLQDFWGRRWNLMTSRILRLVVYDPCRKITIGIVGRKPAAMMAMTATF 241

Query: 243 AVSGLMHELIYFYMGRLRPTWEVTCFFLLHGVCLAAEMTVKTAVGGRFRPPWIVTVPMTI 302
           AVSGLMHELIYFYMGRL PTWEVTCFFLLHGVCLAAEM +K+AVGGRFR P +V+  +TI
Sbjct: 242 AVSGLMHELIYFYMGRLAPTWEVTCFFLLHGVCLAAEMAIKSAVGGRFRLPGVVSAALTI 301

Query: 303 GFIMVTSFWLFFPQFLRFKADVRMFEEHAALGALVKNVTGEWITPLKFS 347
           GF+M TSFWLFFPQFL+ K+DVRMFEE+AALGA VK+VTG+ ITPL  S
Sbjct: 302 GFVMTTSFWLFFPQFLKLKSDVRMFEEYAALGAFVKSVTGQLITPLNIS 350

[Close]

BLAST of Lag0031120 vs. NCBI nr
Match: XP_038903196.1 (acyl-CoA--sterol O-acyltransferase 1-like [Benincasa hispida])

HSP 1 Score: 530.0 bits (1364), Expect = 1.5e-146
Identity = 266/350 (76.00%), Postives = 292/350 (83.43%), Query Frame = 0


Query: 3   ADEIRIFLQAMLQVSSSLCFCFAIRNSIPNGFFKFLLILPLFFLFLYIPLQFHTIHLQGG 62
           A EI   LQ +  VSSSLCFCF IRNSIPNGF KFLLILP FFLFLYIPLQF TIH QG 
Sbjct: 2   AAEIHSLLQTLFLVSSSLCFCFTIRNSIPNGFLKFLLILPFFFLFLYIPLQFQTIHFQGP 61

Query: 63  VGFFIGWLGSFKLLLFAFGKGPLCSAATSSSLRRFLAVGSLPIEIPDEESPPNRSD---- 122
           +GFFIGWLGSFKLLLFAFGKGPL SAATSSSL RFLAVGSLPIEIPD +SPPN S+    
Sbjct: 62  IGFFIGWLGSFKLLLFAFGKGPLSSAATSSSLPRFLAVGSLPIEIPDHQSPPNHSNPKLV 121

Query: 123 -PLTFPAKLGFLILTIAGIYFKNQFHPNAALVFFCLLIYLLLEILIGSAAALAKAALGVE 182
            P ++ AKL FLILT+  I FKN FHPNAAL+F+CLLIY  LE LIGS AA+AKA LGVE
Sbjct: 122 LPFSYSAKLAFLILTMVAINFKNHFHPNAALLFYCLLIYFFLEFLIGSTAAVAKAVLGVE 181

Query: 183 LLPYFHEPYLSSSLQDFWGRRWNLMTSRILRLAVYDPCRKLAVAVVGRKPAVILAVMATF 242
           LLPYF+EPYLS SLQDFWGRRWNLMTSRILRL VYDPCRKL + V+GRKPA +LAVMATF
Sbjct: 182 LLPYFNEPYLSDSLQDFWGRRWNLMTSRILRLTVYDPCRKLTIGVIGRKPATMLAVMATF 241

Query: 243 AVSGLMHELIYFYMGRLRPTWEVTCFFLLHGVCLAAEMTVKTAVGGRFRPPWIVTVPMTI 302
            VSGLMHELIYFY+GRL PTWEVTCFFL+HGVCL  EM VK A GGRFRPP +V++P+TI
Sbjct: 242 VVSGLMHELIYFYIGRLAPTWEVTCFFLVHGVCLVTEMAVKLAAGGRFRPPQVVSIPLTI 301

Query: 303 GFIMVTSFWLFFPQFLRFKADVRMFEEHAALGALVKNVTGEWITPLKFSS 348
           GF+M T  WLFFPQ LR K DVRMFEE+AA+GA VK++T + ITP KF S
Sbjct: 302 GFVMGTGCWLFFPQCLRAKMDVRMFEEYAAIGAFVKSITVDLITPFKFLS 351

[Close]

BLAST of Lag0031120 vs. NCBI nr
Match: KAG7018062.1 (Acyl-CoA--sterol O-acyltransferase 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 529.6 bits (1363), Expect = 2.0e-146
Identity = 269/353 (76.20%), Postives = 302/353 (85.55%), Query Frame = 0


Query: 1   MAADEIRIFLQAMLQVSSSLCFCFAIRNSIPNGFFKFLLILPLFFLFLYIPLQFHTIHLQ 60
           + A EI  F+Q +  ++SSLC CF+IRNSIPNGF KFL +LP FFLFLYIPLQF TIHL+
Sbjct: 187 LMAAEIHAFIQTLFLIASSLCLCFSIRNSIPNGFLKFLFVLPFFFLFLYIPLQFQTIHLR 246

Query: 61  GGVGFFIGWLGSFKLLLFAFGKGPLCSAATSSSLRRFLAVGSLPIEIPDEESPPNRSD-- 120
           G +GFFIGWLGSFKLLLFAFGKGPLC AATSSSLRRFLAV  LPI+IP+ +SPPNRSD  
Sbjct: 247 GTLGFFIGWLGSFKLLLFAFGKGPLCFAATSSSLRRFLAVACLPIDIPEHKSPPNRSDPD 306

Query: 121 ---PLTFPAKLGFLILTIAGIYFKNQFHPNAALVFFCLLIYLLLEILIGSAAALAKAALG 180
              PL+F AKLGFLILT A IYFKN+F+PN  L+FFCLLIYLLLEILIG A ALA+A LG
Sbjct: 307 PLLPLSFAAKLGFLILTFAAIYFKNRFYPNVVLIFFCLLIYLLLEILIGGATALAEAVLG 366

Query: 181 VELLPYFHEPYLSSSLQDFWGRRWNLMTSRILRLAVYDPCRKLAVAVVGRKPAVILAVMA 240
           VELLP+F EPYLS SLQDFWGRRWNL++SRILR AV+DPCRKLA  VVG+KPA I+AVMA
Sbjct: 367 VELLPFFDEPYLSDSLQDFWGRRWNLVSSRILRPAVFDPCRKLAGTVVGKKPAAIIAVMA 426

Query: 241 TFAVSGLMHELIYFYMGRLRPTWEVTCFFLLHGVCLAAEMTVKTAVGGRFRPPWIVTVPM 300
           TFAVSG+MHELIYFYMGRL PTWEV+ FFL+HGVCLAAEM VK+AVGGRFRPP +V+  +
Sbjct: 427 TFAVSGVMHELIYFYMGRLTPTWEVSWFFLIHGVCLAAEMAVKSAVGGRFRPPRVVSGVL 486

Query: 301 TIGFIMVTSFWLFFPQFLRFKADVRMFEEHAALGALVKNVTGEWITPLKFSSF 349
           TIGF+M TSFWLFFPQ LRFKADVRM EE+AALGA VK+VT E I PL F SF
Sbjct: 487 TIGFVMATSFWLFFPQLLRFKADVRMLEEYAALGAFVKSVTAELIGPL-FISF 538

[Close]

BLAST of Lag0031120 vs. NCBI nr
Match: XP_022934181.1 (acyl-CoA--sterol O-acyltransferase 1-like [Cucurbita moschata] >KAG6581558.1 Acyl-CoA--sterol O-acyltransferase 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 529.3 bits (1362), Expect = 2.6e-146
Identity = 269/351 (76.64%), Postives = 301/351 (85.75%), Query Frame = 0


Query: 3   ADEIRIFLQAMLQVSSSLCFCFAIRNSIPNGFFKFLLILPLFFLFLYIPLQFHTIHLQGG 62
           A EI  F+Q +  ++SSLC CF+IRNSIPNGF KFL +LP FFLFLYIPLQF TIHL+G 
Sbjct: 2   AAEIHAFIQTLFLIASSLCLCFSIRNSIPNGFLKFLFVLPFFFLFLYIPLQFQTIHLRGT 61

Query: 63  VGFFIGWLGSFKLLLFAFGKGPLCSAATSSSLRRFLAVGSLPIEIPDEESPPNRSD---- 122
           +GFFIGWLGSFKLLLFAFGKGPLC AATSSSLRRFLAV  LPI+IP+ +SPPNRSD    
Sbjct: 62  LGFFIGWLGSFKLLLFAFGKGPLCFAATSSSLRRFLAVACLPIDIPEHKSPPNRSDPDPL 121

Query: 123 -PLTFPAKLGFLILTIAGIYFKNQFHPNAALVFFCLLIYLLLEILIGSAAALAKAALGVE 182
            PL+F AKLGFLILT A IYFKN+F+PN  L+FFCLLIYLLLEILIG A ALA+A LGVE
Sbjct: 122 LPLSFAAKLGFLILTFAAIYFKNRFYPNVVLIFFCLLIYLLLEILIGGATALAEAVLGVE 181

Query: 183 LLPYFHEPYLSSSLQDFWGRRWNLMTSRILRLAVYDPCRKLAVAVVGRKPAVILAVMATF 242
           LLP+F EPYLS SLQDFWGRRWNL++SRILR AV+DPCRKLA  VVG+KPA I+AVMATF
Sbjct: 182 LLPFFDEPYLSDSLQDFWGRRWNLVSSRILRPAVFDPCRKLAGTVVGKKPAAIIAVMATF 241

Query: 243 AVSGLMHELIYFYMGRLRPTWEVTCFFLLHGVCLAAEMTVKTAVGGRFRPPWIVTVPMTI 302
           AVSG+MHELIYFYMGRL PTWEV+ FFL+HGVCLAAEM VK+AVGGRFRPP +V+  +TI
Sbjct: 242 AVSGVMHELIYFYMGRLTPTWEVSWFFLIHGVCLAAEMAVKSAVGGRFRPPRVVSGVLTI 301

Query: 303 GFIMVTSFWLFFPQFLRFKADVRMFEEHAALGALVKNVTGEWITPLKFSSF 349
           GF+M TSFWLFFPQ LRFKADVRM EE+AALGA VK+VT E I PL F SF
Sbjct: 302 GFVMATSFWLFFPQLLRFKADVRMLEEYAALGAFVKSVTAELIGPL-FISF 351

[Close]

BLAST of Lag0031120 vs. NCBI nr
Match: XP_022982637.1 (acyl-CoA--sterol O-acyltransferase 1-like [Cucurbita maxima])

HSP 1 Score: 526.9 bits (1356), Expect = 1.3e-145
Identity = 268/351 (76.35%), Postives = 301/351 (85.75%), Query Frame = 0


Query: 3   ADEIRIFLQAMLQVSSSLCFCFAIRNSIPNGFFKFLLILPLFFLFLYIPLQFHTIHLQGG 62
           A EI  F+Q +  +SSSLC CF+IRNSIPNGF KF+ +LP FFLFLYIPLQF TIHL+G 
Sbjct: 2   AAEIHAFIQTLFLISSSLCLCFSIRNSIPNGFLKFIFVLPFFFLFLYIPLQFQTIHLRGT 61

Query: 63  VGFFIGWLGSFKLLLFAFGKGPLCSAATSSSLRRFLAVGSLPIEIPDEESPPNRSD---- 122
           +GFFIGWLGSFKLLLFAFGKGPLCSAATSSSLRRFLAV SLPI+IP+ + PPNRSD    
Sbjct: 62  LGFFIGWLGSFKLLLFAFGKGPLCSAATSSSLRRFLAVASLPIDIPEHKPPPNRSDPDPL 121

Query: 123 -PLTFPAKLGFLILTIAGIYFKNQFHPNAALVFFCLLIYLLLEILIGSAAALAKAALGVE 182
            PL+F AKLGFLILT A IYFKN+F+PN  L+FFCLLIYLLLEILIG A A+A+A LGVE
Sbjct: 122 LPLSFAAKLGFLILTFAAIYFKNRFYPNVVLIFFCLLIYLLLEILIGGATAVAEAVLGVE 181

Query: 183 LLPYFHEPYLSSSLQDFWGRRWNLMTSRILRLAVYDPCRKLAVAVVGRKPAVILAVMATF 242
           LLP+F EPYLS SLQDFWGRRWNL++SRILR AV+DPCRKLA  VVG+KPAVI+AVMATF
Sbjct: 182 LLPFFDEPYLSDSLQDFWGRRWNLVSSRILRPAVFDPCRKLAGTVVGKKPAVIIAVMATF 241

Query: 243 AVSGLMHELIYFYMGRLRPTWEVTCFFLLHGVCLAAEMTVKTAVGGRFRPPWIVTVPMTI 302
           AVSG+MHELIYFYMGRL PTWEV+ FFL+HGVCLAAEM VK+AVG RFR P +V+  +T+
Sbjct: 242 AVSGVMHELIYFYMGRLAPTWEVSWFFLIHGVCLAAEMAVKSAVGRRFRAPRVVSGVLTM 301

Query: 303 GFIMVTSFWLFFPQFLRFKADVRMFEEHAALGALVKNVTGEWITPLKFSSF 349
           GF+M TSFWLFFPQ LRFKADVRM EE+AALGA VKNVT E I PL F SF
Sbjct: 302 GFVMATSFWLFFPQLLRFKADVRMLEEYAALGAFVKNVTAELIGPL-FISF 351

[Close]

BLAST of Lag0031120 vs. ExPASy Swiss-Prot
Match: Q9SV07 (Acyl-CoA--sterol O-acyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=ASAT1 PE=1 SV=1)

HSP 1 Score: 257.3 bits (656), Expect = 2.5e-67
Identity = 140/334 (41.92%), Postives = 199/334 (59.58%), Query Frame = 0


Query: 9   FLQAMLQVSSSLCFCFAIRNSIPNGFFKFLLILPLFFLFLYIPLQFHTIHLQGGVGFFIG 68
           F++A   V  SLC+ F I   +P G  + +L  P+F +F  +P   +++HL G   FFI 
Sbjct: 4   FIKAWGLVIISLCYTFFIAKLVPKGIKRLILFFPVFLIFFIVPFLIYSLHLLGITAFFIA 63

Query: 69  WLGSFKLLLFAFGKGPLCSAATSSSLRRFLAVGSLPIEIPDEESPP-------NRSDPLT 128
           WL +FKLLLFA G+GPL S     SL  FLAV  LPI+I     P        +   PL 
Sbjct: 64  WLANFKLLLFALGRGPLSSNHKPLSLPIFLAVSCLPIKIQLSPKPTKTHSHEGSTEGPLI 123

Query: 129 FPAKLGFLILTIAGIYFKNQFHPNAALVFFCLLIYLLLEILIGSAAALAKAALGVELLPY 188
           +  K  F++L I    +  +      L  + + IY  LEI++ + AA  +A   +EL P 
Sbjct: 124 YTIKAVFVVLIIKAYEYSTKLPEKVVLTLYAIHIYFALEIILAATAAAVRAMSDLELEPQ 183

Query: 189 FHEPYLSSSLQDFWGRRWNLMTSRILRLAVYDPCRKLAVAVVGRKPAVILAVMATFAVSG 248
           F++PYL++SLQDFWGRRWNLM + ILR  VY+P  +L  +V+G   + ILA   TF VSG
Sbjct: 184 FNKPYLATSLQDFWGRRWNLMVTGILRPTVYEPSLQL-FSVLGPNYSQILAAFGTFVVSG 243

Query: 249 LMHELIYFYMGRLRPTWEVTCFFLLHGVCLAAEMTVKTAVGGRFRPPWIVTVPMTIGFIM 308
           +MHELI+FYMGRLRP W++  FFL++G C   E+ +K  + GR+R P  ++  +T+ F+M
Sbjct: 244 IMHELIFFYMGRLRPDWKMMWFFLINGFCTTVEIAIKKTINGRWRFPKAISQVLTLTFVM 303

Query: 309 VTSFWLFFPQFLRFKADVRMFEEHAALGALVKNV 336
           VT+ WLF P+F R     +  +E+AA+GA    V
Sbjct: 304 VTALWLFLPEFNRCNIVEKALDEYAAIGAFAVEV 336

[Close]

BLAST of Lag0031120 vs. ExPASy Swiss-Prot
Match: Q9XGY6 (Long-chain-alcohol O-fatty-acyltransferase OS=Simmondsia chinensis OX=3999 PE=1 SV=1)

HSP 1 Score: 237.3 bits (604), Expect = 2.7e-61
Identity = 135/335 (40.30%), Postives = 196/335 (58.51%), Query Frame = 0


Query: 5   EIRIFLQAMLQVSSSLCFCFAIRNSIPN-GFFKFLLILPLFFLFLYIPLQFHTIHLQGGV 64
           E++ F +  +   ++ C+C  +    P+ G  + LL+LP+  LF+++PL+  + HL G  
Sbjct: 6   ELKTFSEVWISAIAAACYCRFVPAVAPHGGALRLLLLLPVVLLFIFLPLRLSSFHLGGPT 65

Query: 65  GFFIGWLGSFKLLLFAFGKGPLCSAATSSSLRRFLAVGSLPIEIPDEESPPNRSDPLT-- 124
             ++ WL +FKLLLFAF  GPL +   S SL  F++   LPI+  D+ S  +  +  T  
Sbjct: 66  ALYLVWLANFKLLLFAFHLGPLSN--PSLSLLHFISTTLLPIKFRDDPSNDHEKNKRTLS 125

Query: 125 --------FPAKLGFLILTIAGIYFKNQFHPNAALVFFCLLIYLLLEILIGSAAALAKAA 184
                   F AKL F    +    F+         V +C   YL  EI + ++A +A+A 
Sbjct: 126 FEWRKVVLFVAKLVFFAGILKIYEFRKDLPHFVISVLYCFHFYLGTEITLAASAVIARAT 185

Query: 185 LGVELLPYFHEPYLSSSLQDFWGRRWNLMTSRILRLAVYDPCRKLAVAVVGRKPAVILAV 244
           LG++L P F+EPYL++SLQDFWGRRWNLM S IL L  Y P R++    V  +  V  A+
Sbjct: 186 LGLDLYPQFNEPYLATSLQDFWGRRWNLMVSDILGLTTYQPVRRVLSRWVRLRWEVAGAM 245

Query: 245 MATFAVSGLMHELIYFYMGRLRPTWEVTCFFLLHGVCLAAEMTVKTAVGGRFRPPWIVTV 304
           +  F VSGLMHE+ +FY+ R RP+WEVT FF+LHGVC A EM VK AV G+ R    V+ 
Sbjct: 246 LVAFTVSGLMHEVFFFYLTRARPSWEVTGFFVLHGVCTAVEMVVKKAVSGKVRLRREVSG 305

Query: 305 PMTIGFIMVTSFWLFFPQFLRFKADVRMFEEHAAL 329
            +T+GF+MVT  WLF PQ +R   D++  +E+  +
Sbjct: 306 ALTVGFVMVTGGWLFLPQLVRHGVDLKTIDEYPVM 338

[Close]

BLAST of Lag0031120 vs. ExPASy Swiss-Prot
Match: Q9FJ75 (Probable long-chain-alcohol O-fatty-acyltransferase 4 OS=Arabidopsis thaliana OX=3702 GN=AT4 PE=2 SV=1)

HSP 1 Score: 229.6 bits (584), Expect = 5.6e-59
Identity = 132/332 (39.76%), Postives = 189/332 (56.93%), Query Frame = 0


Query: 4   DEIRIFLQAMLQVSSSLCFCFAIRNSIPNGFFKFLLILPLFFLFLYIPLQFHTIHLQGGV 63
           +E+   ++  +    S+ +C+     I +G F+ L +LP+  LFL +PL   ++H  G  
Sbjct: 3   EELMSLIKVWVYAIISISYCYYTSTRIKSGVFRLLSVLPVCVLFLVLPLFVSSVHFSGST 62

Query: 64  GFFIGWLGSFKLLLFAFGKGPLCSAATSSSLRRFLAVGSLPIEIPDEESPPNRSDPLTFP 123
            FF+ WL +FKL+LF+F +GPL      S+L RF+     PI++     P N+     F 
Sbjct: 63  AFFLSWLANFKLILFSFDQGPL--FPVPSNLSRFVCFTCFPIKLQQNPKPQNQMPKWGFA 122

Query: 124 AKLGFLILTIAGIYFKNQFHPNAALVFFCLLIYLLLEILIGSAAALAKAALGVELLPYFH 183
            KL F  + +    +K    P   LV + L IYL  EIL+     L   +L  +L P+F+
Sbjct: 123 VKLAFFGVLLHMYEYKQHMSPTVLLVLYSLHIYLEYEILLAPLKVLLSISLWCDLEPHFN 182

Query: 184 EPYLSSSLQDFWGRRWNLMTSRILRLAVYDPCRKLAVAVVGRKPAVILAVMATFAVSGLM 243
           EPYLS+SLQDFWGRRWNLM   ILR AVY P R++A   +    A+ L V A+F VSG++
Sbjct: 183 EPYLSTSLQDFWGRRWNLMVPAILRPAVYLPVRQMAGRKMNSDQALFLGVFASFLVSGVV 242

Query: 244 HELIYFYMGRLRPTWEVTCFFLLHGVCLAAEMTVK-TAVGGRFRPPWIVTVPMTIGFIMV 303
           HELI+FY  R  PT EVT FF+LHGVC AAE   K T +  R++   +V+  +T+GF+++
Sbjct: 243 HELIFFYFTRESPTGEVTLFFVLHGVCTAAECAAKRTRLVRRWKVSQMVSRLLTVGFVVM 302

Query: 304 TSFWLFFPQFLRFKADVRMFEEHAALGALVKN 335
           T  WLFFP   R     R+ +E       VK+
Sbjct: 303 TGGWLFFPHLARSGMIERLADEAFLFIGFVKH 332

[Close]

BLAST of Lag0031120 vs. ExPASy Swiss-Prot
Match: Q9FJ74 (Probable long-chain-alcohol O-fatty-acyltransferase 3 OS=Arabidopsis thaliana OX=3702 GN=AT3 PE=3 SV=1)

HSP 1 Score: 215.7 bits (548), Expect = 8.4e-55
Identity = 128/310 (41.29%), Postives = 181/310 (58.39%), Query Frame = 0


Query: 4   DEIRIFLQAMLQVSSSLCFCFAIRNSIPNGFFKFLLILPLFFLFLYIPLQFHTIHLQGGV 63
           +E++ F++  +    S+ +C+ +   I  G F+ L +LP+  LFL  PL F  +H  G +
Sbjct: 3   EELKNFIKLWISAIISISYCYYLSTGIKAGVFRLLSVLPVCALFLVFPLFFSYVHFSGCM 62

Query: 64  GFFIGWLGSFKLLLFAFGKGPLCSAATSSSLRRFLAVGSLPIEIPDEESPPNRSDPL-TF 123
            FF+ WL +FKL+LF+F +GPL  +    +L RF+ +   PI+     +  N   P+  F
Sbjct: 63  AFFLSWLANFKLILFSFDQGPL--SPLPRTLSRFICITCFPIKPQQNPNIQNYKIPIWLF 122

Query: 124 PAKLGFLILTIAGIYFKNQFHPNAALVFFCLLIYLLLEILIGSAAALAKAALGVELLPYF 183
             K+   ++ +    +K    P   LVF  L I+L LEI+     AL    LG +L P  
Sbjct: 123 AIKVVIFVVLLQMYEYKQYLSPALLLVFNSLHIFLELEIVFMLVKALVFITLGCDLEPQS 182

Query: 184 HEPYLSSSLQDFWGRRWNLMTSRILRLAVYDPCRKLAVAVVGRKPAVILAVMATFAVSGL 243
           +EPYL++SLQDFWGRRWNLM   ILR AVY P R++A   V    A+ L V A F VSG 
Sbjct: 183 NEPYLATSLQDFWGRRWNLMVPAILRPAVYLPARRMACRKVNSDQAMFLGVFAAFLVSGA 242

Query: 244 MHELIYFYMGRLRPTWEVTCFFLLHGVCLAAEMTVK--TAVGGRFRPPWIVTVPMTIGFI 303
           +HE+++FY+ R  PT EVT FFLLHGVC  AE+ VK  T V   +R    V+  +T+GF+
Sbjct: 243 VHEMLFFYLTREVPTGEVTWFFLLHGVCTVAEVAVKKSTFVRRWWRVSPTVSRLLTVGFV 302

Query: 304 MVTSFWLFFP 311
           +VTS W FFP
Sbjct: 303 VVTSGWFFFP 310

[Close]

BLAST of Lag0031120 vs. ExPASy Swiss-Prot
Match: Q9FJ76 (Probable long-chain-alcohol O-fatty-acyltransferase 5 OS=Arabidopsis thaliana OX=3702 GN=AT5 PE=2 SV=1)

HSP 1 Score: 207.2 bits (526), Expect = 3.0e-52
Identity = 124/332 (37.35%), Postives = 183/332 (55.12%), Query Frame = 0


Query: 4   DEIRIFLQAMLQVSSSLCFCFAIRNSIPNGFFKFLLILPLFFLFLYIPLQFHTIHLQGGV 63
           +E++  ++  +    S+ +C+ I   I +G  +FL + P+  LFL +PL F ++HL    
Sbjct: 3   EELKNLIKVWVSAIISISYCYYIPPRIKSGAPRFLSVSPVLALFLVLPLFFSSLHLSLIT 62

Query: 64  GFFIGWLGSFKLLLFAFGKGPLCSAATSSSLRRFLAVGSLPIEIPDEESPPNRSDPLTFP 123
            FF+ WL +FKL+LF+F KGPL    T  +  RF      PI++       N    L F 
Sbjct: 63  AFFLTWLANFKLILFSFDKGPLIPIPT--NFPRFFCFTCFPIKVQQNPKSQNHLPKLVFA 122

Query: 124 AKLGFLILTIAGIYFKNQFHPNAALVFFCLLIYLLLEILIGSAAALAKAALGVELLPYFH 183
            KL    + +    ++    P   L  + + +YL +EI++     +   +LG +L P  +
Sbjct: 123 IKLAIFAVLLHLYSYRQNLSPTILLGLYFVHLYLEIEIILTFVKVVVFISLGCDLEPQSN 182

Query: 184 EPYLSSSLQDFWGRRWNLMTSRILRLAVYDPCRKLAVAVVGRKPAVILAVMATFAVSGLM 243
           +PYL++SLQDFWGRRWNLM   ILR AVY P R+++   +    A+   ++A F VSGL+
Sbjct: 183 KPYLATSLQDFWGRRWNLMVPAILRPAVYAPMRRVSERKMSSGWALFPGILAAFIVSGLV 242

Query: 244 HELIYFYMGRLRPTWEVTCFFLLHGVCLAAEMTV--KTAVGGRFRPPWIVTVPMTIGFIM 303
           HEL++FY+ R  PT EVT FF+LHGVC A E+ V  KT V  R+R    V+  +T+GF+ 
Sbjct: 243 HELLFFYLIREMPTGEVTLFFVLHGVCTAVELAVKKKTTVAQRWRLSPGVSRVLTVGFVF 302

Query: 304 VTSFWLFFPQFLRFKADVRMFEEHAALGALVK 334
           VT  WLF PQ  R     R   E   L   +K
Sbjct: 303 VTGGWLFTPQLKRSGVMERFTSEAVLLVEFIK 332

[Close]

BLAST of Lag0031120 vs. ExPASy TrEMBL
Match: A0A6J1F144 (acyl-CoA--sterol O-acyltransferase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111441426 PE=3 SV=1)

HSP 1 Score: 529.3 bits (1362), Expect = 1.3e-146
Identity = 269/351 (76.64%), Postives = 301/351 (85.75%), Query Frame = 0


Query: 3   ADEIRIFLQAMLQVSSSLCFCFAIRNSIPNGFFKFLLILPLFFLFLYIPLQFHTIHLQGG 62
           A EI  F+Q +  ++SSLC CF+IRNSIPNGF KFL +LP FFLFLYIPLQF TIHL+G 
Sbjct: 2   AAEIHAFIQTLFLIASSLCLCFSIRNSIPNGFLKFLFVLPFFFLFLYIPLQFQTIHLRGT 61

Query: 63  VGFFIGWLGSFKLLLFAFGKGPLCSAATSSSLRRFLAVGSLPIEIPDEESPPNRSD---- 122
           +GFFIGWLGSFKLLLFAFGKGPLC AATSSSLRRFLAV  LPI+IP+ +SPPNRSD    
Sbjct: 62  LGFFIGWLGSFKLLLFAFGKGPLCFAATSSSLRRFLAVACLPIDIPEHKSPPNRSDPDPL 121

Query: 123 -PLTFPAKLGFLILTIAGIYFKNQFHPNAALVFFCLLIYLLLEILIGSAAALAKAALGVE 182
            PL+F AKLGFLILT A IYFKN+F+PN  L+FFCLLIYLLLEILIG A ALA+A LGVE
Sbjct: 122 LPLSFAAKLGFLILTFAAIYFKNRFYPNVVLIFFCLLIYLLLEILIGGATALAEAVLGVE 181

Query: 183 LLPYFHEPYLSSSLQDFWGRRWNLMTSRILRLAVYDPCRKLAVAVVGRKPAVILAVMATF 242
           LLP+F EPYLS SLQDFWGRRWNL++SRILR AV+DPCRKLA  VVG+KPA I+AVMATF
Sbjct: 182 LLPFFDEPYLSDSLQDFWGRRWNLVSSRILRPAVFDPCRKLAGTVVGKKPAAIIAVMATF 241

Query: 243 AVSGLMHELIYFYMGRLRPTWEVTCFFLLHGVCLAAEMTVKTAVGGRFRPPWIVTVPMTI 302
           AVSG+MHELIYFYMGRL PTWEV+ FFL+HGVCLAAEM VK+AVGGRFRPP +V+  +TI
Sbjct: 242 AVSGVMHELIYFYMGRLTPTWEVSWFFLIHGVCLAAEMAVKSAVGGRFRPPRVVSGVLTI 301

Query: 303 GFIMVTSFWLFFPQFLRFKADVRMFEEHAALGALVKNVTGEWITPLKFSSF 349
           GF+M TSFWLFFPQ LRFKADVRM EE+AALGA VK+VT E I PL F SF
Sbjct: 302 GFVMATSFWLFFPQLLRFKADVRMLEEYAALGAFVKSVTAELIGPL-FISF 351

[Close]

BLAST of Lag0031120 vs. ExPASy TrEMBL
Match: A0A6J1IX47 (acyl-CoA--sterol O-acyltransferase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111481451 PE=3 SV=1)

HSP 1 Score: 526.9 bits (1356), Expect = 6.3e-146
Identity = 268/351 (76.35%), Postives = 301/351 (85.75%), Query Frame = 0


Query: 3   ADEIRIFLQAMLQVSSSLCFCFAIRNSIPNGFFKFLLILPLFFLFLYIPLQFHTIHLQGG 62
           A EI  F+Q +  +SSSLC CF+IRNSIPNGF KF+ +LP FFLFLYIPLQF TIHL+G 
Sbjct: 2   AAEIHAFIQTLFLISSSLCLCFSIRNSIPNGFLKFIFVLPFFFLFLYIPLQFQTIHLRGT 61

Query: 63  VGFFIGWLGSFKLLLFAFGKGPLCSAATSSSLRRFLAVGSLPIEIPDEESPPNRSD---- 122
           +GFFIGWLGSFKLLLFAFGKGPLCSAATSSSLRRFLAV SLPI+IP+ + PPNRSD    
Sbjct: 62  LGFFIGWLGSFKLLLFAFGKGPLCSAATSSSLRRFLAVASLPIDIPEHKPPPNRSDPDPL 121

Query: 123 -PLTFPAKLGFLILTIAGIYFKNQFHPNAALVFFCLLIYLLLEILIGSAAALAKAALGVE 182
            PL+F AKLGFLILT A IYFKN+F+PN  L+FFCLLIYLLLEILIG A A+A+A LGVE
Sbjct: 122 LPLSFAAKLGFLILTFAAIYFKNRFYPNVVLIFFCLLIYLLLEILIGGATAVAEAVLGVE 181

Query: 183 LLPYFHEPYLSSSLQDFWGRRWNLMTSRILRLAVYDPCRKLAVAVVGRKPAVILAVMATF 242
           LLP+F EPYLS SLQDFWGRRWNL++SRILR AV+DPCRKLA  VVG+KPAVI+AVMATF
Sbjct: 182 LLPFFDEPYLSDSLQDFWGRRWNLVSSRILRPAVFDPCRKLAGTVVGKKPAVIIAVMATF 241

Query: 243 AVSGLMHELIYFYMGRLRPTWEVTCFFLLHGVCLAAEMTVKTAVGGRFRPPWIVTVPMTI 302
           AVSG+MHELIYFYMGRL PTWEV+ FFL+HGVCLAAEM VK+AVG RFR P +V+  +T+
Sbjct: 242 AVSGVMHELIYFYMGRLAPTWEVSWFFLIHGVCLAAEMAVKSAVGRRFRAPRVVSGVLTM 301

Query: 303 GFIMVTSFWLFFPQFLRFKADVRMFEEHAALGALVKNVTGEWITPLKFSSF 349
           GF+M TSFWLFFPQ LRFKADVRM EE+AALGA VKNVT E I PL F SF
Sbjct: 302 GFVMATSFWLFFPQLLRFKADVRMLEEYAALGAFVKNVTAELIGPL-FISF 351

[Close]

BLAST of Lag0031120 vs. ExPASy TrEMBL
Match: A0A6J1DI64 (acyl-CoA--sterol O-acyltransferase 1-like OS=Momordica charantia OX=3673 GN=LOC111020278 PE=3 SV=1)

HSP 1 Score: 522.7 bits (1345), Expect = 1.2e-144
Identity = 265/348 (76.15%), Postives = 299/348 (85.92%), Query Frame = 0


Query: 1   MAADEIRIFLQAMLQVSSSLCFCFAIRNSIPNGFFKFLLILPLFFLFLYIPLQFHTIHLQ 60
           + A EI IF+Q +L V SSLCFCF+IRNSIP+GF +FLLILP FFLFL IPLQFHTIHLQ
Sbjct: 218 LMATEIGIFVQTVLLVCSSLCFCFSIRNSIPHGFLRFLLILPFFFLFLCIPLQFHTIHLQ 277

Query: 61  GGVGFFIGWLGSFKLLLFAFGKGPLCSAATSSSLRRFLAVGSLPIEIP--DEESPPNRSD 120
           G +GFFIGWLGSFKLLLFAFGKGPLCSA  ++SLRRFLAVGSLPIEIP    ++P   S 
Sbjct: 278 GAIGFFIGWLGSFKLLLFAFGKGPLCSAPAAASLRRFLAVGSLPIEIPVHPPQAPAPDSP 337

Query: 121 P---LTFPAKLGFLILTIAGIYFKNQFHPNAALVFFCLLIYLLLEILIGSAAALAKAALG 180
           P   LTFPAKLG L+LT   IYF+N F+P A LVF+CLLIYLLLEIL+G AAALA+  LG
Sbjct: 338 PVLSLTFPAKLGLLLLTFCAIYFRNYFNPYAVLVFYCLLIYLLLEILVGGAAALARTLLG 397

Query: 181 VELLPYFHEPYLSSSLQDFWGRRWNLMTSRILRLAVYDPCRKLAVAVVGRKPAVILAVMA 240
           V+L PYF EPYLS+SLQ+FWGRRWNLMT+RILR+AVYDPCR++A A VG++ AV+LAV A
Sbjct: 398 VDLSPYFDEPYLSASLQEFWGRRWNLMTTRILRVAVYDPCRRVAGAAVGKRAAVMLAVAA 457

Query: 241 TFAVSGLMHELIYFYMGRLRPTWEVTCFFLLHGVCLAAEMTVKTAVGGRFRPPWIVTVPM 300
           TFAVSGLMHELIYFYMGR+ PTWEVTCFFLLHGVCL AEM VK A GGRFR P  V+ P+
Sbjct: 458 TFAVSGLMHELIYFYMGRMAPTWEVTCFFLLHGVCLPAEMAVKAAAGGRFRLPRAVSAPL 517

Query: 301 TIGFIMVTSFWLFFPQFLRFKADVRMFEEHAALGALVKNVTGEWITPL 344
           T+GF+MVTSFWLFFPQFLRFKADVRMFEE+AALGALVKNVTG+ ITPL
Sbjct: 518 TLGFVMVTSFWLFFPQFLRFKADVRMFEEYAALGALVKNVTGDMITPL 565

[Close]

BLAST of Lag0031120 vs. ExPASy TrEMBL
Match: A0A6J1KCL2 (acyl-CoA--sterol O-acyltransferase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111493090 PE=3 SV=1)

HSP 1 Score: 518.5 bits (1334), Expect = 2.2e-143
Identity = 267/351 (76.07%), Postives = 289/351 (82.34%), Query Frame = 0


Query: 3   ADEIRIFLQAMLQVSSSLCFCFAIRNSIPNGFFKFLLILPLFFLFLYIPLQFHTIHLQGG 62
           A EI IFLQ +LQVSSSLCFCF+IRNSIPNGF KFLLILP F LFLYIPLQF T+HLQG 
Sbjct: 2   AAEILIFLQTLLQVSSSLCFCFSIRNSIPNGFLKFLLILPFFLLFLYIPLQFQTLHLQGA 61

Query: 63  VGFFIGWLGSFKLLLFAFGKGPLCSAATSSSLRRFLAVGSLPIEIPDEESPPNRSD---- 122
           +GFFIGWL SFKLLLFAFGKG LCSAA SSSL RFL VGSLPI+I D +    R D    
Sbjct: 62  IGFFIGWLASFKLLLFAFGKGSLCSAAASSSLSRFLLVGSLPIDIYDHDPDSTRPDTTSV 121

Query: 123 -PLTFPAKLGFLILTIAGIYFKNQFHPNAALVFFCLLIYLLLEILIGSAAALAKAALGVE 182
            PL+  +KL FLIL+IA IY KN FHPN  L+ FC +IYLLLEI++G AAA+AKA LGVE
Sbjct: 122 LPLSLSSKLFFLILSIAAIYCKNYFHPNGTLIVFCFVIYLLLEIVLGGAAAVAKATLGVE 181

Query: 183 LLPYFHEPYLSSSLQDFWGRRWNLMTSRILRLAVYDPCRKLAVAVVGRKPAVILAVMATF 242
           LLP F EPYLSSSLQDFWGRRWNLM SRILRL+VYDPCR  A A+VGRKPA I+A+ ATF
Sbjct: 182 LLPCFDEPYLSSSLQDFWGRRWNLMVSRILRLSVYDPCRNFAAAIVGRKPAAIMAMTATF 241

Query: 243 AVSGLMHELIYFYMGRLRPTWEVTCFFLLHGVCLAAEMTVKTAVGGRFRPPWIVTVPMTI 302
           AVSGLMHELIYFYMGRL PTWEVT FFL+HG  LA EM VK AVG RFRPP IVTVP+TI
Sbjct: 242 AVSGLMHELIYFYMGRLAPTWEVTSFFLVHGFFLAVEMAVKNAVGERFRPPRIVTVPLTI 301

Query: 303 GFIMVTSFWLFFPQFLRFKADVRMFEEHAALGALVKNVTGEWITPLKFSSF 349
           GF+MVTSFWLFFPQF RFK DVRMFEEHAALGA VKNV GE IT +K  SF
Sbjct: 302 GFVMVTSFWLFFPQFFRFKPDVRMFEEHAALGAFVKNVAGELITSVKLISF 352

[Close]

BLAST of Lag0031120 vs. ExPASy TrEMBL
Match: A0A6J1GBB6 (acyl-CoA--sterol O-acyltransferase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111452615 PE=3 SV=1)

HSP 1 Score: 517.3 bits (1331), Expect = 5.0e-143
Identity = 265/351 (75.50%), Postives = 288/351 (82.05%), Query Frame = 0


Query: 3   ADEIRIFLQAMLQVSSSLCFCFAIRNSIPNGFFKFLLILPLFFLFLYIPLQFHTIHLQGG 62
           A EI IFLQ +LQVSSSLCFCF+IRNSIPNGF KFLLILP F LFLYIPLQF T+HLQG 
Sbjct: 2   AAEILIFLQTLLQVSSSLCFCFSIRNSIPNGFLKFLLILPFFLLFLYIPLQFQTLHLQGA 61

Query: 63  VGFFIGWLGSFKLLLFAFGKGPLCSAATSSSLRRFLAVGSLPIEIPDEESPPNRSD---- 122
           +GFFIGWL SFKLLLFAFGKGPLCSA  SSSLRRFL VGSLPI+I D +S   R D    
Sbjct: 62  IGFFIGWLASFKLLLFAFGKGPLCSAVASSSLRRFLLVGSLPIDIYDHDSASTRPDTNSV 121

Query: 123 -PLTFPAKLGFLILTIAGIYFKNQFHPNAALVFFCLLIYLLLEILIGSAAALAKAALGVE 182
            PL+  +KL FL L+IA IY KN FHPN  L+ FC +IYLLLEI++G AAA+AKA LGVE
Sbjct: 122 LPLSLSSKLFFLSLSIAAIYCKNYFHPNGTLIVFCFVIYLLLEIVLGGAAAVAKATLGVE 181

Query: 183 LLPYFHEPYLSSSLQDFWGRRWNLMTSRILRLAVYDPCRKLAVAVVGRKPAVILAVMATF 242
           LLP F EPYLSSSLQDFWGRRWNLM SRILRL+VYDPCR  A A+VGRKPA I+A  ATF
Sbjct: 182 LLPCFDEPYLSSSLQDFWGRRWNLMVSRILRLSVYDPCRNFAAAIVGRKPAAIMATTATF 241

Query: 243 AVSGLMHELIYFYMGRLRPTWEVTCFFLLHGVCLAAEMTVKTAVGGRFRPPWIVTVPMTI 302
           AVSGLMHELIYFY+GRL PTWEVT FFL+HG  LA EM VK AVG RFRPP IVTVP+TI
Sbjct: 242 AVSGLMHELIYFYLGRLAPTWEVTSFFLVHGFFLAVEMAVKNAVGERFRPPRIVTVPLTI 301

Query: 303 GFIMVTSFWLFFPQFLRFKADVRMFEEHAALGALVKNVTGEWITPLKFSSF 349
           GF+MVTSFWLFFPQF RFK DVRMFEEHAA+GA VKNV  E IT +K  SF
Sbjct: 302 GFVMVTSFWLFFPQFFRFKPDVRMFEEHAAMGAFVKNVAAELITSVKLISF 352

[Close]

BLAST of Lag0031120 vs. TAIR 10
Match: AT3G51970.1 (acyl-CoA sterol acyl transferase 1 )

HSP 1 Score: 257.3 bits (656), Expect = 1.8e-68
Identity = 140/334 (41.92%), Postives = 199/334 (59.58%), Query Frame = 0


Query: 9   FLQAMLQVSSSLCFCFAIRNSIPNGFFKFLLILPLFFLFLYIPLQFHTIHLQGGVGFFIG 68
           F++A   V  SLC+ F I   +P G  + +L  P+F +F  +P   +++HL G   FFI 
Sbjct: 4   FIKAWGLVIISLCYTFFIAKLVPKGIKRLILFFPVFLIFFIVPFLIYSLHLLGITAFFIA 63

Query: 69  WLGSFKLLLFAFGKGPLCSAATSSSLRRFLAVGSLPIEIPDEESPP-------NRSDPLT 128
           WL +FKLLLFA G+GPL S     SL  FLAV  LPI+I     P        +   PL 
Sbjct: 64  WLANFKLLLFALGRGPLSSNHKPLSLPIFLAVSCLPIKIQLSPKPTKTHSHEGSTEGPLI 123

Query: 129 FPAKLGFLILTIAGIYFKNQFHPNAALVFFCLLIYLLLEILIGSAAALAKAALGVELLPY 188
           +  K  F++L I    +  +      L  + + IY  LEI++ + AA  +A   +EL P 
Sbjct: 124 YTIKAVFVVLIIKAYEYSTKLPEKVVLTLYAIHIYFALEIILAATAAAVRAMSDLELEPQ 183

Query: 189 FHEPYLSSSLQDFWGRRWNLMTSRILRLAVYDPCRKLAVAVVGRKPAVILAVMATFAVSG 248
           F++PYL++SLQDFWGRRWNLM + ILR  VY+P  +L  +V+G   + ILA   TF VSG
Sbjct: 184 FNKPYLATSLQDFWGRRWNLMVTGILRPTVYEPSLQL-FSVLGPNYSQILAAFGTFVVSG 243

Query: 249 LMHELIYFYMGRLRPTWEVTCFFLLHGVCLAAEMTVKTAVGGRFRPPWIVTVPMTIGFIM 308
           +MHELI+FYMGRLRP W++  FFL++G C   E+ +K  + GR+R P  ++  +T+ F+M
Sbjct: 244 IMHELIFFYMGRLRPDWKMMWFFLINGFCTTVEIAIKKTINGRWRFPKAISQVLTLTFVM 303

Query: 309 VTSFWLFFPQFLRFKADVRMFEEHAALGALVKNV 336
           VT+ WLF P+F R     +  +E+AA+GA    V
Sbjct: 304 VTALWLFLPEFNRCNIVEKALDEYAAIGAFAVEV 336

[Close]

BLAST of Lag0031120 vs. TAIR 10
Match: AT5G55350.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 229.6 bits (584), Expect = 4.0e-60
Identity = 132/332 (39.76%), Postives = 189/332 (56.93%), Query Frame = 0


Query: 4   DEIRIFLQAMLQVSSSLCFCFAIRNSIPNGFFKFLLILPLFFLFLYIPLQFHTIHLQGGV 63
           +E+   ++  +    S+ +C+     I +G F+ L +LP+  LFL +PL   ++H  G  
Sbjct: 3   EELMSLIKVWVYAIISISYCYYTSTRIKSGVFRLLSVLPVCVLFLVLPLFVSSVHFSGST 62

Query: 64  GFFIGWLGSFKLLLFAFGKGPLCSAATSSSLRRFLAVGSLPIEIPDEESPPNRSDPLTFP 123
            FF+ WL +FKL+LF+F +GPL      S+L RF+     PI++     P N+     F 
Sbjct: 63  AFFLSWLANFKLILFSFDQGPL--FPVPSNLSRFVCFTCFPIKLQQNPKPQNQMPKWGFA 122

Query: 124 AKLGFLILTIAGIYFKNQFHPNAALVFFCLLIYLLLEILIGSAAALAKAALGVELLPYFH 183
            KL F  + +    +K    P   LV + L IYL  EIL+     L   +L  +L P+F+
Sbjct: 123 VKLAFFGVLLHMYEYKQHMSPTVLLVLYSLHIYLEYEILLAPLKVLLSISLWCDLEPHFN 182

Query: 184 EPYLSSSLQDFWGRRWNLMTSRILRLAVYDPCRKLAVAVVGRKPAVILAVMATFAVSGLM 243
           EPYLS+SLQDFWGRRWNLM   ILR AVY P R++A   +    A+ L V A+F VSG++
Sbjct: 183 EPYLSTSLQDFWGRRWNLMVPAILRPAVYLPVRQMAGRKMNSDQALFLGVFASFLVSGVV 242

Query: 244 HELIYFYMGRLRPTWEVTCFFLLHGVCLAAEMTVK-TAVGGRFRPPWIVTVPMTIGFIMV 303
           HELI+FY  R  PT EVT FF+LHGVC AAE   K T +  R++   +V+  +T+GF+++
Sbjct: 243 HELIFFYFTRESPTGEVTLFFVLHGVCTAAECAAKRTRLVRRWKVSQMVSRLLTVGFVVM 302

Query: 304 TSFWLFFPQFLRFKADVRMFEEHAALGALVKN 335
           T  WLFFP   R     R+ +E       VK+
Sbjct: 303 TGGWLFFPHLARSGMIERLADEAFLFIGFVKH 332

[Close]

BLAST of Lag0031120 vs. TAIR 10
Match: AT5G55360.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 215.7 bits (548), Expect = 5.9e-56
Identity = 128/310 (41.29%), Postives = 181/310 (58.39%), Query Frame = 0


Query: 4   DEIRIFLQAMLQVSSSLCFCFAIRNSIPNGFFKFLLILPLFFLFLYIPLQFHTIHLQGGV 63
           +E++ F++  +    S+ +C+ +   I  G F+ L +LP+  LFL  PL F  +H  G +
Sbjct: 3   EELKNFIKLWISAIISISYCYYLSTGIKAGVFRLLSVLPVCALFLVFPLFFSYVHFSGCM 62

Query: 64  GFFIGWLGSFKLLLFAFGKGPLCSAATSSSLRRFLAVGSLPIEIPDEESPPNRSDPL-TF 123
            FF+ WL +FKL+LF+F +GPL  +    +L RF+ +   PI+     +  N   P+  F
Sbjct: 63  AFFLSWLANFKLILFSFDQGPL--SPLPRTLSRFICITCFPIKPQQNPNIQNYKIPIWLF 122

Query: 124 PAKLGFLILTIAGIYFKNQFHPNAALVFFCLLIYLLLEILIGSAAALAKAALGVELLPYF 183
             K+   ++ +    +K    P   LVF  L I+L LEI+     AL    LG +L P  
Sbjct: 123 AIKVVIFVVLLQMYEYKQYLSPALLLVFNSLHIFLELEIVFMLVKALVFITLGCDLEPQS 182

Query: 184 HEPYLSSSLQDFWGRRWNLMTSRILRLAVYDPCRKLAVAVVGRKPAVILAVMATFAVSGL 243
           +EPYL++SLQDFWGRRWNLM   ILR AVY P R++A   V    A+ L V A F VSG 
Sbjct: 183 NEPYLATSLQDFWGRRWNLMVPAILRPAVYLPARRMACRKVNSDQAMFLGVFAAFLVSGA 242

Query: 244 MHELIYFYMGRLRPTWEVTCFFLLHGVCLAAEMTVK--TAVGGRFRPPWIVTVPMTIGFI 303
           +HE+++FY+ R  PT EVT FFLLHGVC  AE+ VK  T V   +R    V+  +T+GF+
Sbjct: 243 VHEMLFFYLTREVPTGEVTWFFLLHGVCTVAEVAVKKSTFVRRWWRVSPTVSRLLTVGFV 302

Query: 304 MVTSFWLFFP 311
           +VTS W FFP
Sbjct: 303 VVTSGWFFFP 310

[Close]

BLAST of Lag0031120 vs. TAIR 10
Match: AT5G55340.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 207.2 bits (526), Expect = 2.1e-53
Identity = 124/332 (37.35%), Postives = 183/332 (55.12%), Query Frame = 0


Query: 4   DEIRIFLQAMLQVSSSLCFCFAIRNSIPNGFFKFLLILPLFFLFLYIPLQFHTIHLQGGV 63
           +E++  ++  +    S+ +C+ I   I +G  +FL + P+  LFL +PL F ++HL    
Sbjct: 3   EELKNLIKVWVSAIISISYCYYIPPRIKSGAPRFLSVSPVLALFLVLPLFFSSLHLSLIT 62

Query: 64  GFFIGWLGSFKLLLFAFGKGPLCSAATSSSLRRFLAVGSLPIEIPDEESPPNRSDPLTFP 123
            FF+ WL +FKL+LF+F KGPL    T  +  RF      PI++       N    L F 
Sbjct: 63  AFFLTWLANFKLILFSFDKGPLIPIPT--NFPRFFCFTCFPIKVQQNPKSQNHLPKLVFA 122

Query: 124 AKLGFLILTIAGIYFKNQFHPNAALVFFCLLIYLLLEILIGSAAALAKAALGVELLPYFH 183
            KL    + +    ++    P   L  + + +YL +EI++     +   +LG +L P  +
Sbjct: 123 IKLAIFAVLLHLYSYRQNLSPTILLGLYFVHLYLEIEIILTFVKVVVFISLGCDLEPQSN 182

Query: 184 EPYLSSSLQDFWGRRWNLMTSRILRLAVYDPCRKLAVAVVGRKPAVILAVMATFAVSGLM 243
           +PYL++SLQDFWGRRWNLM   ILR AVY P R+++   +    A+   ++A F VSGL+
Sbjct: 183 KPYLATSLQDFWGRRWNLMVPAILRPAVYAPMRRVSERKMSSGWALFPGILAAFIVSGLV 242

Query: 244 HELIYFYMGRLRPTWEVTCFFLLHGVCLAAEMTV--KTAVGGRFRPPWIVTVPMTIGFIM 303
           HEL++FY+ R  PT EVT FF+LHGVC A E+ V  KT V  R+R    V+  +T+GF+ 
Sbjct: 243 HELLFFYLIREMPTGEVTLFFVLHGVCTAVELAVKKKTTVAQRWRLSPGVSRVLTVGFVF 302

Query: 304 VTSFWLFFPQFLRFKADVRMFEEHAALGALVK 334
           VT  WLF PQ  R     R   E   L   +K
Sbjct: 303 VTGGWLFTPQLKRSGVMERFTSEAVLLVEFIK 332

[Close]

BLAST of Lag0031120 vs. TAIR 10
Match: AT5G55370.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 203.0 bits (515), Expect = 4.0e-52
Identity = 124/333 (37.24%), Postives = 181/333 (54.35%), Query Frame = 0


Query: 4   DEIRIFLQAMLQVSSSLCFCFAIRNSIPNGFFKFLLILPLFFLFLYIPLQFHTIHLQGGV 63
           +E+R  ++  +    S+ +C+ I + I  G  + L +LP+F +FL +PL F ++H     
Sbjct: 3   EELRNLIKVWISALISISYCYYISSKISKGVLRLLSLLPIFIIFLLLPLFFSSVHFCVIS 62

Query: 64  GFFIGWLGSFKLLLFAFGKGPLCSAATSSSLRRFLAVGSLPIEIPDEESPPNRSDPLTFP 123
           GFF  WL +FKL LFAF + PL  +   S+L RF      PI+I    S     +     
Sbjct: 63  GFFFTWLANFKLFLFAFDQEPL--SPLPSNLTRFFCFACFPIKINKNPSSNRIHNKPMSK 122

Query: 124 AKLGFLILTIAGI--YFKNQFHPN----AALVFFCLLIYLLLEILIGSAAALAKAALGVE 183
             L F +L  + +   ++N +       A L  F + +YL  E+++    AL    LG E
Sbjct: 123 WVLAFKLLIFSFLLHVYRNNYDSGLSRFAFLALFTIHVYLEAELILVFVGALMSMLLGCE 182

Query: 184 LLPYFHEPYLSSSLQDFWGRRWNLMTSRILRLAVYDPCRKLAVAVVGRKPAVILAVMATF 243
           + P F++PYL++SLQ+FW RRWNLM   +LR AV+ P ++    ++G   A    ++ATF
Sbjct: 183 MEPVFNDPYLATSLQEFWSRRWNLMVPAVLRPAVHIPVQRFCAPLLGLHRAFYAGMLATF 242

Query: 244 AVSGLMHELIYFYMGRLRPTWEVTCFFLLHGVCLAAEMTVKTAVGGRFRPPWIVTVPMTI 303
            VSGLMHELIYFY+ R  PTWEVTCFFLLHGV    E+ +K       R  W+ T    +
Sbjct: 243 IVSGLMHELIYFYVIRKSPTWEVTCFFLLHGVVTCLEIAMK-------RMRWLPTPRRAV 302

Query: 304 G------FIMVTSFWLFFPQFLRFKADVRMFEE 325
                  F++VT+ WLF+PQ LR     R+  E
Sbjct: 303 SGLAITVFLLVTAGWLFYPQMLRNDVHKRVISE 326

The following BLAST results are available for this feature:

BLAST of Lag0031120 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038905685.1	7.8e-151	77.94	acyl-CoA--sterol O-acyltransferase 1-like [Benincasa hispida]	[more]
XP_038903196.1	1.5e-146	76.00	acyl-CoA--sterol O-acyltransferase 1-like [Benincasa hispida]	[more]
KAG7018062.1	2.0e-146	76.20	Acyl-CoA--sterol O-acyltransferase 1, partial [Cucurbita argyrosperma subsp. arg...	[more]
XP_022934181.1	2.6e-146	76.64	acyl-CoA--sterol O-acyltransferase 1-like [Cucurbita moschata] >KAG6581558.1 Acy...	[more]
XP_022982637.1	1.3e-145	76.35	acyl-CoA--sterol O-acyltransferase 1-like [Cucurbita maxima]	[more]

BLAST of Lag0031120 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SV07	2.5e-67	41.92	Acyl-CoA--sterol O-acyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=ASAT1 PE...	[more]
Q9XGY6	2.7e-61	40.30	Long-chain-alcohol O-fatty-acyltransferase OS=Simmondsia chinensis OX=3999 PE=1 ...	[more]
Q9FJ75	5.6e-59	39.76	Probable long-chain-alcohol O-fatty-acyltransferase 4 OS=Arabidopsis thaliana OX...	[more]
Q9FJ74	8.4e-55	41.29	Probable long-chain-alcohol O-fatty-acyltransferase 3 OS=Arabidopsis thaliana OX...	[more]
Q9FJ76	3.0e-52	37.35	Probable long-chain-alcohol O-fatty-acyltransferase 5 OS=Arabidopsis thaliana OX...	[more]

BLAST of Lag0031120 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1F144	1.3e-146	76.64	acyl-CoA--sterol O-acyltransferase 1-like OS=Cucurbita moschata OX=3662 GN=LOC11...	[more]
A0A6J1IX47	6.3e-146	76.35	acyl-CoA--sterol O-acyltransferase 1-like OS=Cucurbita maxima OX=3661 GN=LOC1114...	[more]
A0A6J1DI64	1.2e-144	76.15	acyl-CoA--sterol O-acyltransferase 1-like OS=Momordica charantia OX=3673 GN=LOC1...	[more]
A0A6J1KCL2	2.2e-143	76.07	acyl-CoA--sterol O-acyltransferase 1-like OS=Cucurbita maxima OX=3661 GN=LOC1114...	[more]
A0A6J1GBB6	5.0e-143	75.50	acyl-CoA--sterol O-acyltransferase 1-like OS=Cucurbita moschata OX=3662 GN=LOC11...	[more]

BLAST of Lag0031120 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G51970.1	1.8e-68	41.92	acyl-CoA sterol acyl transferase 1	[more]
AT5G55350.1	4.0e-60	39.76	MBOAT (membrane bound O-acyl transferase) family protein	[more]
AT5G55360.1	5.9e-56	41.29	MBOAT (membrane bound O-acyl transferase) family protein	[more]
AT5G55340.1	2.1e-53	37.35	MBOAT (membrane bound O-acyl transferase) family protein	[more]
AT5G55370.1	4.0e-52	37.24	MBOAT (membrane bound O-acyl transferase) family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR032805	Wax synthase domain	PFAM	PF13813	MBOAT_2	coord: 180..265 e-value: 4.8E-17 score: 62.0
None	No IPR available	PANTHER	PTHR31595:SF46	ACYL-COA--STEROL O-ACYLTRANSFERASE 1	coord: 4..336
IPR044851	Wax synthase	PANTHER	PTHR31595	LONG-CHAIN-ALCOHOL O-FATTY-ACYLTRANSFERASE 3-RELATED	coord: 4..336

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0031120.1	Lag0031120.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006629	lipid metabolic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0008374	O-acyltransferase activity