Lag0031118 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0031118
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	Hydroxymethylbilane synthase
Location	chr11: 4993853 .. 4996500 (-)
RNA-Seq Expression	Lag0031118
Synteny	Lag0031118

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGTGTTTTATCTTCCCCTTCTATAAGCCACTCTCCAATGGCTCCTCCTTCTAATGTTCGCTCCCTTTCCCTTCTTGGGTTCTCCTCTCTTTCCCTTAAACCTCCAGCTTTCTCTAATGGCGCCAAGAAATTTCATGGGTTTCCCCTTATAAGAGCCTCCGTTGCTGTGGAGCAGCAGACAGAGAAGACCAAGGTCGCCCTCGTCAGAATTGGCACCAGAGGAAGGTGATTTTTGGGTCTTTTTTAGATTTCCTTTTCCTTTGATGGTTTTGAAATGTCTTTAGTTTCTAAATTTTGAGTTTCGTAATGTGGGGCATTGTGTTGTTTGTTGAAGGGTTTGGATCAGTTCAATTTTTGTTGATATCTAAGTAGAATTTGAAAGTTTTCAGTATGCTTGTCATGATTCTGGTGCTGTGGAAACTTGTATGTGTCACTCTGGCTCCACTGAAGGAGTACTTTTCTTGCTTGGATAGAGAGGTTTTCTTATCAAGAGTGGTAGTTGCAGTTTTTCTTATGGTTGTGAATCAGATTATCAAGAATGCTAAGGAGTGGAAAATGAACCAAATCAAAGAAAACAATAGATTCCTGAGGACTAACAATGGTGGAGAATCTCCCCTTCCTCCTTTTGTGTGCTTGAGTTCTGAATTGATTAAGTTTAGGAAAAGTATTCATGTTGCTAACGGCATCGATCATGGTAGTGTTGTTTTAACTTACAATCTAAATGTTAGGATCCAATCTGCTTCTGTTAGAGTTGAATAAGATCTTTATTAGAAGAGCAAGTTTTTGCTGGTAGGAACTAAGGGGCTTGTAATTTTCTTATCGAAGCTGGGAACTATGTCTATGAACTTAAAGTACTTCATTCTCTAGCTGGTTATATCCTTGTTTGATCTAAATCTTGTGCTGGATTGCTTTATCTTCTTCTGTGTCACATATATTTTTCTTGTGTAGGACAGTCCATTAGCCCTTGCCCAGGCTCATGAGACAAGAGACAAACTAATGGCTATCCATCCCGAGCTAGCCGAAGATGGGGCCATTCAGATTGTTATAATCAAAACAACCGGTGACAAAATACTCTCTCAGCCACTTGCAGACATTGGTGGAAAAGGCTTATTTACTAAAGAGATAGATGATGCGCTTATTAATGGCGACATAGACATTGCTGTTCACTCAATGAAAGATGTACCGACTTACTTACCTGAAAAAACCATCCTCCCGTGTAATCTTCCAAGGGAAGATGTCCGTGATGCATTCATTTCTCTAAGTGCGGGTTCACTCGCTGAGCTTCCAGCTGGAAGCGTCATTGGTACAGCTTCACTGAGAAGAAAGTCTCAGCTACTGAATAGATATCCATCCCTCAATGTAAGTATGCTGTGTTGTTTAGCAGAACAATGTCCAAAAGTTTATGTTTTTATGTTTACATCGCTGCAAGAGTTAATAAGTTGTAGATCTCAGCCTCTAATGAATGAAATTGGTTCCTTAAAGATATGATAGAAAGAATGTCTCTTTCGTTTCCTTTCTGCCTTGATTGATCTTTTAAAATGTTGTTCTGCATTACTGCTTCTATGTTGTCTCAACAAATTCCTTGTTTTTCCTTCTTATTCATTGCTGATACAATGAGTTGTTGCATTGTTCGACAATCGACGATTACCGGTTATTTTCTTATAGGCACTTGAAGATTCTCTGCTTCCCCCACCTTTCGTTTCGTTAAATTTAGCATTCCTGTCTCTTGATGGAACCATTTAGGACTCTGTGATTTTAAGGATGTTAAAGAGTCTTAAAGTCTTCAATATTGTGGGTTGATTCTGATACTCTCTCTTGTTTTTTCCCCCGGTTGGTTGGTGTCATTTGGCAGGTGCTGGAGAATTTTCGGGGCAATGTCCAAACAAGGTTGAGAAAACTGAATGAAGGAGTGGTCCAAGCAACACTATTGGCTCTAGCTGGACTTCGGCGTTTAAATATGACAGAAAACGTTACTTCGATCCTTTCAATAGATGAAATGCTTCCAGCTGTTGCTCAGGGAGCAATCGGGATTGCCTGTCGAAGTGATGATGACAAAATGGTACGTTTTCATCGGCTATGGACACTACATCTAGCTGAGAAGACAATTGTTTCTACCTGTCTTATCACCATGTACCATTAAAATTCATAGCTTGAGAAGAAAAAACTGAATGATATCTTCTTATTTCTCTTGGAGTTGAACTTTTTGGTTTCTCATTTGCAGGCCAATTACCTAGCCTCATTAAACCATGAGGAAACAAGACTGGCTGTTGTTTGTGAGAGAGCATTCCTTGAGACTCTTGATGGATCTTGCCGAACTCCGATCGCAGGATATGCTTCCAGAGATGAAGATGGCAATTGTATATTTAAAGGGTTGGTGGCTTCCCCGGATGGAACTCGAGGTACGAACAACGTCCATGTTCAGATCAAATAACTTTTACTCTCATTCTCATTAGTCATTAGCTTCTCCTCCATTGCTAGACCATTGAATATCTTCATATTTTAAGCCTTACCCCTGTTCCTTCCTCCATTTCAGTTCTTGAAACTTCTCGACGAGGCCTATACGCCATCGACGATATGATTGCAATGGGAAAGGATGCTGGGAAGGAGCTTCTTTCTCGAGCAGGTCCCGGTTTTTTTGATAGCTAG

mRNA sequence

ATGGGTGTTTTATCTTCCCCTTCTATAAGCCACTCTCCAATGGCTCCTCCTTCTAATGTTCGCTCCCTTTCCCTTCTTGGGTTCTCCTCTCTTTCCCTTAAACCTCCAGCTTTCTCTAATGGCGCCAAGAAATTTCATGGGTTTCCCCTTATAAGAGCCTCCGTTGCTGTGGAGCAGCAGACAGAGAAGACCAAGGTCGCCCTCGTCAGAATTGGCACCAGAGGAAGTATGCTTGTCATGATTCTGGTGCTGTGGAAACTTGTATGTGTCACTCTGGCTCCACTGAAGGAGTACTTTTCTTGCTTGGATAGAGAGGTTTTCTTATCAAGAGTGGTAGTTGCAGTTTTTCTTATGGTTGTGAATCAGATTATCAAGAATGCTAAGGAGTGGAAAATGAACCAAATCAAAGAAAACAATAGATTCCTGAGGACTAACAATGGTGGAGAATCTCCCCTTCCTCCTTTTGTGTGCTTGAGTTCTGAATTGATTAAGTTTAGGAAAAGTATTCATGTTGCTAACGGCATCGATCATGGTAGTGTTGTTTTAACTTACAATCTAAATGTTAGGATCCAATCTGCTTCTGTTAGAGACAGTCCATTAGCCCTTGCCCAGGCTCATGAGACAAGAGACAAACTAATGGCTATCCATCCCGAGCTAGCCGAAGATGGGGCCATTCAGATTGTTATAATCAAAACAACCGGTGACAAAATACTCTCTCAGCCACTTGCAGACATTGGTGGAAAAGGCTTATTTACTAAAGAGATAGATGATGCGCTTATTAATGGCGACATAGACATTGCTGTTCACTCAATGAAAGATGTACCGACTTACTTACCTGAAAAAACCATCCTCCCGTGTAATCTTCCAAGGGAAGATGTCCGTGATGCATTCATTTCTCTAAGTGCGGGTTCACTCGCTGAGCTTCCAGCTGGAAGCGTCATTGGTACAGCTTCACTGAGAAGAAAGTCTCAGCTACTGAATAGATATCCATCCCTCAATGTGCTGGAGAATTTTCGGGGCAATGTCCAAACAAGGTTGAGAAAACTGAATGAAGGAGTGGTCCAAGCAACACTATTGGCTCTAGCTGGACTTCGGCGTTTAAATATGACAGAAAACGTTACTTCGATCCTTTCAATAGATGAAATGCTTCCAGCTGTTGCTCAGGGAGCAATCGGGATTGCCTGTCGAAGTGATGATGACAAAATGGCCAATTACCTAGCCTCATTAAACCATGAGGAAACAAGACTGGCTGTTGTTTGTGAGAGAGCATTCCTTGAGACTCTTGATGGATCTTGCCGAACTCCGATCGCAGGATATGCTTCCAGAGATGAAGATGGCAATTGTATATTTAAAGGGTTGGTGGCTTCCCCGGATGGAACTCGAGTTCTTGAAACTTCTCGACGAGGCCTATACGCCATCGACGATATGATTGCAATGGGAAAGGATGCTGGGAAGGAGCTTCTTTCTCGAGCAGGTCCCGGTTTTTTTGATAGCTAG

Coding sequence (CDS)

ATGGGTGTTTTATCTTCCCCTTCTATAAGCCACTCTCCAATGGCTCCTCCTTCTAATGTTCGCTCCCTTTCCCTTCTTGGGTTCTCCTCTCTTTCCCTTAAACCTCCAGCTTTCTCTAATGGCGCCAAGAAATTTCATGGGTTTCCCCTTATAAGAGCCTCCGTTGCTGTGGAGCAGCAGACAGAGAAGACCAAGGTCGCCCTCGTCAGAATTGGCACCAGAGGAAGTATGCTTGTCATGATTCTGGTGCTGTGGAAACTTGTATGTGTCACTCTGGCTCCACTGAAGGAGTACTTTTCTTGCTTGGATAGAGAGGTTTTCTTATCAAGAGTGGTAGTTGCAGTTTTTCTTATGGTTGTGAATCAGATTATCAAGAATGCTAAGGAGTGGAAAATGAACCAAATCAAAGAAAACAATAGATTCCTGAGGACTAACAATGGTGGAGAATCTCCCCTTCCTCCTTTTGTGTGCTTGAGTTCTGAATTGATTAAGTTTAGGAAAAGTATTCATGTTGCTAACGGCATCGATCATGGTAGTGTTGTTTTAACTTACAATCTAAATGTTAGGATCCAATCTGCTTCTGTTAGAGACAGTCCATTAGCCCTTGCCCAGGCTCATGAGACAAGAGACAAACTAATGGCTATCCATCCCGAGCTAGCCGAAGATGGGGCCATTCAGATTGTTATAATCAAAACAACCGGTGACAAAATACTCTCTCAGCCACTTGCAGACATTGGTGGAAAAGGCTTATTTACTAAAGAGATAGATGATGCGCTTATTAATGGCGACATAGACATTGCTGTTCACTCAATGAAAGATGTACCGACTTACTTACCTGAAAAAACCATCCTCCCGTGTAATCTTCCAAGGGAAGATGTCCGTGATGCATTCATTTCTCTAAGTGCGGGTTCACTCGCTGAGCTTCCAGCTGGAAGCGTCATTGGTACAGCTTCACTGAGAAGAAAGTCTCAGCTACTGAATAGATATCCATCCCTCAATGTGCTGGAGAATTTTCGGGGCAATGTCCAAACAAGGTTGAGAAAACTGAATGAAGGAGTGGTCCAAGCAACACTATTGGCTCTAGCTGGACTTCGGCGTTTAAATATGACAGAAAACGTTACTTCGATCCTTTCAATAGATGAAATGCTTCCAGCTGTTGCTCAGGGAGCAATCGGGATTGCCTGTCGAAGTGATGATGACAAAATGGCCAATTACCTAGCCTCATTAAACCATGAGGAAACAAGACTGGCTGTTGTTTGTGAGAGAGCATTCCTTGAGACTCTTGATGGATCTTGCCGAACTCCGATCGCAGGATATGCTTCCAGAGATGAAGATGGCAATTGTATATTTAAAGGGTTGGTGGCTTCCCCGGATGGAACTCGAGTTCTTGAAACTTCTCGACGAGGCCTATACGCCATCGACGATATGATTGCAATGGGAAAGGATGCTGGGAAGGAGCTTCTTTCTCGAGCAGGTCCCGGTTTTTTTGATAGCTAG

Protein sequence

MGVLSSPSISHSPMAPPSNVRSLSLLGFSSLSLKPPAFSNGAKKFHGFPLIRASVAVEQQTEKTKVALVRIGTRGSMLVMILVLWKLVCVTLAPLKEYFSCLDREVFLSRVVVAVFLMVVNQIIKNAKEWKMNQIKENNRFLRTNNGGESPLPPFVCLSSELIKFRKSIHVANGIDHGSVVLTYNLNVRIQSASVRDSPLALAQAHETRDKLMAIHPELAEDGAIQIVIIKTTGDKILSQPLADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSAGSLAELPAGSVIGTASLRRKSQLLNRYPSLNVLENFRGNVQTRLRKLNEGVVQATLLALAGLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDKMANYLASLNHEETRLAVVCERAFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGLYAIDDMIAMGKDAGKELLSRAGPGFFDS

Homology

[Close]

BLAST of Lag0031118 vs. NCBI nr
Match: XP_038906286.1 (porphobilinogen deaminase, chloroplastic [Benincasa hispida])

HSP 1 Score: 653.3 bits (1684), Expect = 1.7e-183
Identity = 364/498 (73.09%), Postives = 368/498 (73.90%), Query Frame = 0


Query: 1   MGVLSSPSISHSPMAPPSNVRSLSLLGFSSLSLKPPAFSNGAKKFHGFPLIRASVAVEQQ 60
           MGVLSSPS+SHSPM  P NV SLSLLGFSSLSLKPPAFSNGAKKFHGF LIRASVAVEQQ
Sbjct: 1   MGVLSSPSVSHSPMPRPRNVGSLSLLGFSSLSLKPPAFSNGAKKFHGFGLIRASVAVEQQ 60

Query: 61  TEKTKVALVRIGTRGSMLVMILVLWKLVCVTLAPLKEYFSCLDREVFLSRVVVAVFLMVV 120
           TEKTKVALVRIGTRG                                             
Sbjct: 61  TEKTKVALVRIGTRG--------------------------------------------- 120

Query: 121 NQIIKNAKEWKMNQIKENNRFLRTNNGGESPLPPFVCLSSELIKFRKSIHVANGIDHGSV 180
                                                                       
Sbjct: 121 ------------------------------------------------------------ 180

Query: 181 VLTYNLNVRIQSASVRDSPLALAQAHETRDKLMAIHPELAEDGAIQIVIIKTTGDKILSQ 240
                            SPLALAQAHETRDKLMA HPELAEDGAIQIV+IKTTGDKILSQ
Sbjct: 181 -----------------SPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQ 240

Query: 241 PLADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISL 300
           PLADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISL
Sbjct: 241 PLADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISL 300

Query: 301 SAGSLAELPAGSVIGTASLRRKSQLLNRYPSLNVLENFRGNVQTRLRKLNEGVVQATLLA 360
           SAGSLAELPAGS+IGTASLRRKSQLLNRYPSL VLENFRGNVQTRLRKLNEGVVQATLLA
Sbjct: 301 SAGSLAELPAGSIIGTASLRRKSQLLNRYPSLKVLENFRGNVQTRLRKLNEGVVQATLLA 360

Query: 361 LAGLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDKMANYLASLNHEETRLAVVC 420
           LAGLRRLNMTENVTSILSIDEMLPAVAQGAIGIACR+DDDKMANYLASLNHEETRLAVVC
Sbjct: 361 LAGLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRTDDDKMANYLASLNHEETRLAVVC 376

Query: 421 ERAFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGLYAIDDMIAMG 480
           ERAFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRG YAIDDMIAMG
Sbjct: 421 ERAFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGPYAIDDMIAMG 376

Query: 481 KDAGKELLSRAGPGFFDS 499
           KDAGKELLSRAGPGFFDS
Sbjct: 481 KDAGKELLSRAGPGFFDS 376

[Close]

BLAST of Lag0031118 vs. NCBI nr
Match: XP_023523547.1 (porphobilinogen deaminase, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 642.5 bits (1656), Expect = 3.0e-180
Identity = 361/498 (72.49%), Postives = 363/498 (72.89%), Query Frame = 0


Query: 1   MGVLSSPSISHSPMAPPSNVRSLSLLGFSSLSLKPPAFSNGAKKFHGFPLIRASVAVEQQ 60
           MGV SSPSISH PMA P N+ SLS  GFSSLSLKPPAFS GAKKFHGF LIRASVAVEQQ
Sbjct: 18  MGVFSSPSISHYPMARPCNLGSLSRHGFSSLSLKPPAFSTGAKKFHGFGLIRASVAVEQQ 77

Query: 61  TEKTKVALVRIGTRGSMLVMILVLWKLVCVTLAPLKEYFSCLDREVFLSRVVVAVFLMVV 120
            EKTKVALVRIGTRG                                             
Sbjct: 78  AEKTKVALVRIGTRG--------------------------------------------- 137

Query: 121 NQIIKNAKEWKMNQIKENNRFLRTNNGGESPLPPFVCLSSELIKFRKSIHVANGIDHGSV 180
                                                                       
Sbjct: 138 ------------------------------------------------------------ 197

Query: 181 VLTYNLNVRIQSASVRDSPLALAQAHETRDKLMAIHPELAEDGAIQIVIIKTTGDKILSQ 240
                            SPLALAQAHETR+KLMAIHPELAEDGAIQIVIIKTTGDKILSQ
Sbjct: 198 -----------------SPLALAQAHETREKLMAIHPELAEDGAIQIVIIKTTGDKILSQ 257

Query: 241 PLADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISL 300
           PLADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISL
Sbjct: 258 PLADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISL 317

Query: 301 SAGSLAELPAGSVIGTASLRRKSQLLNRYPSLNVLENFRGNVQTRLRKLNEGVVQATLLA 360
           S GSLAELPAGSVIGTASLRRKSQLLNRYPSLNVLENFRGNVQTRLRKLNEGVVQATLLA
Sbjct: 318 SVGSLAELPAGSVIGTASLRRKSQLLNRYPSLNVLENFRGNVQTRLRKLNEGVVQATLLA 377

Query: 361 LAGLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDKMANYLASLNHEETRLAVVC 420
           LAGLRRLNMTE VTSILSIDEMLPAVAQGAIGIACRSDDDKMANYLASLNHEETRLAVVC
Sbjct: 378 LAGLRRLNMTETVTSILSIDEMLPAVAQGAIGIACRSDDDKMANYLASLNHEETRLAVVC 393

Query: 421 ERAFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGLYAIDDMIAMG 480
           ERAFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRG YAIDDMIAMG
Sbjct: 438 ERAFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGPYAIDDMIAMG 393

Query: 481 KDAGKELLSRAGPGFFDS 499
           KDAGKELLSRAGPGFFDS
Sbjct: 498 KDAGKELLSRAGPGFFDS 393

[Close]

BLAST of Lag0031118 vs. NCBI nr
Match: XP_022949290.1 (porphobilinogen deaminase, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 641.0 bits (1652), Expect = 8.7e-180
Identity = 360/498 (72.29%), Postives = 362/498 (72.69%), Query Frame = 0


Query: 1   MGVLSSPSISHSPMAPPSNVRSLSLLGFSSLSLKPPAFSNGAKKFHGFPLIRASVAVEQQ 60
           MGV SSPSISH PMA P N+ SLS  GFSSLSLKPPAFS G KKFHGF LIRASVAVEQQ
Sbjct: 1   MGVFSSPSISHYPMARPCNLGSLSRHGFSSLSLKPPAFSTGGKKFHGFGLIRASVAVEQQ 60

Query: 61  TEKTKVALVRIGTRGSMLVMILVLWKLVCVTLAPLKEYFSCLDREVFLSRVVVAVFLMVV 120
            EKTKVALVRIGTRG                                             
Sbjct: 61  AEKTKVALVRIGTRG--------------------------------------------- 120

Query: 121 NQIIKNAKEWKMNQIKENNRFLRTNNGGESPLPPFVCLSSELIKFRKSIHVANGIDHGSV 180
                                                                       
Sbjct: 121 ------------------------------------------------------------ 180

Query: 181 VLTYNLNVRIQSASVRDSPLALAQAHETRDKLMAIHPELAEDGAIQIVIIKTTGDKILSQ 240
                            SPLALAQAHETR+KLMAIHPELAEDGAIQIVIIKTTGDKILSQ
Sbjct: 181 -----------------SPLALAQAHETREKLMAIHPELAEDGAIQIVIIKTTGDKILSQ 240

Query: 241 PLADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISL 300
           PLADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISL
Sbjct: 241 PLADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISL 300

Query: 301 SAGSLAELPAGSVIGTASLRRKSQLLNRYPSLNVLENFRGNVQTRLRKLNEGVVQATLLA 360
           S GSLAELPAGSVIGTASLRRKSQLLNRYPSLNVLENFRGNVQTRLRKLNEGVVQATLLA
Sbjct: 301 SVGSLAELPAGSVIGTASLRRKSQLLNRYPSLNVLENFRGNVQTRLRKLNEGVVQATLLA 360

Query: 361 LAGLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDKMANYLASLNHEETRLAVVC 420
           LAGLRRLNMTE VTSILSIDEMLPAVAQGAIGIACRSDDDKMANYLASLNHEETRLAVVC
Sbjct: 361 LAGLRRLNMTETVTSILSIDEMLPAVAQGAIGIACRSDDDKMANYLASLNHEETRLAVVC 376

Query: 421 ERAFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGLYAIDDMIAMG 480
           ERAFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRG YAIDDMIAMG
Sbjct: 421 ERAFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGPYAIDDMIAMG 376

Query: 481 KDAGKELLSRAGPGFFDS 499
           KDAGKELLSRAGPGFFDS
Sbjct: 481 KDAGKELLSRAGPGFFDS 376

[Close]

BLAST of Lag0031118 vs. NCBI nr
Match: KAG7036601.1 (Porphobilinogen deaminase, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 639.8 bits (1649), Expect = 1.9e-179
Identity = 359/498 (72.09%), Postives = 362/498 (72.69%), Query Frame = 0


Query: 1   MGVLSSPSISHSPMAPPSNVRSLSLLGFSSLSLKPPAFSNGAKKFHGFPLIRASVAVEQQ 60
           MGV SSPSISH PMA P N+ SLS  GFSSLSLKPPAFS G KKFHGF LIRASVAVEQQ
Sbjct: 1   MGVFSSPSISHYPMARPCNLGSLSRHGFSSLSLKPPAFSTGGKKFHGFGLIRASVAVEQQ 60

Query: 61  TEKTKVALVRIGTRGSMLVMILVLWKLVCVTLAPLKEYFSCLDREVFLSRVVVAVFLMVV 120
            EKTKVALVRIGTRG                                             
Sbjct: 61  AEKTKVALVRIGTRG--------------------------------------------- 120

Query: 121 NQIIKNAKEWKMNQIKENNRFLRTNNGGESPLPPFVCLSSELIKFRKSIHVANGIDHGSV 180
                                                                       
Sbjct: 121 ------------------------------------------------------------ 180

Query: 181 VLTYNLNVRIQSASVRDSPLALAQAHETRDKLMAIHPELAEDGAIQIVIIKTTGDKILSQ 240
                            SPLALAQAHETR+KLMAIHPELAEDGAIQIVIIKTTGDKILSQ
Sbjct: 181 -----------------SPLALAQAHETREKLMAIHPELAEDGAIQIVIIKTTGDKILSQ 240

Query: 241 PLADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISL 300
           PLADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISL
Sbjct: 241 PLADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISL 300

Query: 301 SAGSLAELPAGSVIGTASLRRKSQLLNRYPSLNVLENFRGNVQTRLRKLNEGVVQATLLA 360
           S GSLAELPAGSVIGTASLRRKSQLLNRYPSLNVLENFRGNVQTRLRKLNEGVVQATLLA
Sbjct: 301 SVGSLAELPAGSVIGTASLRRKSQLLNRYPSLNVLENFRGNVQTRLRKLNEGVVQATLLA 360

Query: 361 LAGLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDKMANYLASLNHEETRLAVVC 420
           LAGLRRLNMTE VTSILSIDEMLPAVAQGAIGIACRSDDDKMANYL+SLNHEETRLAVVC
Sbjct: 361 LAGLRRLNMTETVTSILSIDEMLPAVAQGAIGIACRSDDDKMANYLSSLNHEETRLAVVC 376

Query: 421 ERAFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGLYAIDDMIAMG 480
           ERAFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRG YAIDDMIAMG
Sbjct: 421 ERAFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGPYAIDDMIAMG 376

Query: 481 KDAGKELLSRAGPGFFDS 499
           KDAGKELLSRAGPGFFDS
Sbjct: 481 KDAGKELLSRAGPGFFDS 376

[Close]

BLAST of Lag0031118 vs. NCBI nr
Match: KAG6606898.1 (Porphobilinogen deaminase, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 638.3 bits (1645), Expect = 5.7e-179
Identity = 358/498 (71.89%), Postives = 362/498 (72.69%), Query Frame = 0


Query: 1   MGVLSSPSISHSPMAPPSNVRSLSLLGFSSLSLKPPAFSNGAKKFHGFPLIRASVAVEQQ 60
           MGV SSPSISH PMA P N+ SLS  GFSSLSLKPPAFS G KKFHGF LIRASVAVEQQ
Sbjct: 1   MGVFSSPSISHYPMARPCNLGSLSRHGFSSLSLKPPAFSTGGKKFHGFGLIRASVAVEQQ 60

Query: 61  TEKTKVALVRIGTRGSMLVMILVLWKLVCVTLAPLKEYFSCLDREVFLSRVVVAVFLMVV 120
            EKTKVALVRIGTRG                                             
Sbjct: 61  AEKTKVALVRIGTRG--------------------------------------------- 120

Query: 121 NQIIKNAKEWKMNQIKENNRFLRTNNGGESPLPPFVCLSSELIKFRKSIHVANGIDHGSV 180
                                                                       
Sbjct: 121 ------------------------------------------------------------ 180

Query: 181 VLTYNLNVRIQSASVRDSPLALAQAHETRDKLMAIHPELAEDGAIQIVIIKTTGDKILSQ 240
                            SPLALAQAHETR+KLMAIHPELAEDGAIQIVIIKTTGDKILSQ
Sbjct: 181 -----------------SPLALAQAHETREKLMAIHPELAEDGAIQIVIIKTTGDKILSQ 240

Query: 241 PLADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISL 300
           PLADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISL
Sbjct: 241 PLADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISL 300

Query: 301 SAGSLAELPAGSVIGTASLRRKSQLLNRYPSLNVLENFRGNVQTRLRKLNEGVVQATLLA 360
           S GSLAELPAGSVIGTASLRRKSQLLNRYPSLNVLENFRGNVQTRLRKLNEGVVQATLLA
Sbjct: 301 SVGSLAELPAGSVIGTASLRRKSQLLNRYPSLNVLENFRGNVQTRLRKLNEGVVQATLLA 360

Query: 361 LAGLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDKMANYLASLNHEETRLAVVC 420
           LAGLRRLNMTE VTSILSIDEMLPAVAQGAIGIACRSDDDKMANYL+SLNHEETRLAVVC
Sbjct: 361 LAGLRRLNMTETVTSILSIDEMLPAVAQGAIGIACRSDDDKMANYLSSLNHEETRLAVVC 376

Query: 421 ERAFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGLYAIDDMIAMG 480
           ERAFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDG+RVLETSRRG YAIDDMIAMG
Sbjct: 421 ERAFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGSRVLETSRRGPYAIDDMIAMG 376

Query: 481 KDAGKELLSRAGPGFFDS 499
           KDAGKELLSRAGPGFFDS
Sbjct: 481 KDAGKELLSRAGPGFFDS 376

[Close]

BLAST of Lag0031118 vs. ExPASy Swiss-Prot
Match: Q43082 (Porphobilinogen deaminase, chloroplastic OS=Pisum sativum OX=3888 GN=HEMC PE=1 SV=1)

HSP 1 Score: 528.9 bits (1361), Expect = 6.3e-149
Identity = 267/303 (88.12%), Postives = 288/303 (95.05%), Query Frame = 0


Query: 196 RDSPLALAQAHETRDKLMAIHPELAEDGAIQIVIIKTTGDKILSQPLADIGGKGLFTKEI 255
           R SPLALAQAHETRDKLMA H ELAE+GAIQIVIIKTTGDKILSQPLADIGGKGLFTKEI
Sbjct: 67  RGSPLALAQAHETRDKLMASHTELAEEGAIQIVIIKTTGDKILSQPLADIGGKGLFTKEI 126

Query: 256 DDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSAGSLAELPAGSVIG 315
           D+ALINGDIDIAVHSMKDVPTYLPE+TILPCNLPREDVRDAFISLSA SLA+LPAGSVIG
Sbjct: 127 DEALINGDIDIAVHSMKDVPTYLPEETILPCNLPREDVRDAFISLSAASLADLPAGSVIG 186

Query: 316 TASLRRKSQLLNRYPSLNVLENFRGNVQTRLRKLNEGVVQATLLALAGLRRLNMTENVTS 375
           TASLRRKSQ+L+RYPSL V +NFRGNVQTRLRKL+EGVV+ATLLALAGL+RLNMTENVTS
Sbjct: 187 TASLRRKSQILHRYPSLTVQDNFRGNVQTRLRKLSEGVVKATLLALAGLKRLNMTENVTS 246

Query: 376 ILSIDEMLPAVAQGAIGIACRSDDDKMANYLASLNHEETRLAVVCERAFLETLDGSCRTP 435
            LSID+MLPAVAQGAIGIACRS+DDKMA YLASLNHEETRLA+ CERAFL TLDGSCRTP
Sbjct: 247 TLSIDDMLPAVAQGAIGIACRSNDDKMAEYLASLNHEETRLAISCERAFLTTLDGSCRTP 306

Query: 436 IAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGLYAIDDMIAMGKDAGKELLSRAGPGF 495
           IAGYASRD+DGNC+F+GLVASPDGTRVLETSR G Y  +DM+ +GKDAG+ELLSRAGPGF
Sbjct: 307 IAGYASRDKDGNCLFRGLVASPDGTRVLETSRIGSYTYEDMMKIGKDAGEELLSRAGPGF 366

Query: 496 FDS 499
           F+S
Sbjct: 367 FNS 369

[Close]

BLAST of Lag0031118 vs. ExPASy Swiss-Prot
Match: Q43316 (Porphobilinogen deaminase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=HEMC PE=1 SV=1)

HSP 1 Score: 510.0 bits (1312), Expect = 3.0e-143
Identity = 258/325 (79.38%), Postives = 285/325 (87.69%), Query Frame = 0


Query: 172 ANGIDHGSVVLTYNLNVRIQSASVRDSPLALAQAHETRDKLMAIHPELAEDGAIQIVIIK 231
           ++G     V +       I     R SPLALAQA+ETR+KL   HPEL EDGAI I IIK
Sbjct: 56  SSGFVKACVAVEQKTRTAIIRIGTRGSPLALAQAYETREKLKKKHPELVEDGAIHIEIIK 115

Query: 232 TTGDKILSQPLADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPRE 291
           TTGDKILSQPLADIGGKGLFTKEID+ALING IDIAVHSMKDVPTYLPEKTILPCNLPRE
Sbjct: 116 TTGDKILSQPLADIGGKGLFTKEIDEALINGHIDIAVHSMKDVPTYLPEKTILPCNLPRE 175

Query: 292 DVRDAFISLSAGSLAELPAGSVIGTASLRRKSQLLNRYPSLNVLENFRGNVQTRLRKLNE 351
           DVRDAFI L+A +LAELPAGSV+GTASLRRKSQ+L++YP+L+V ENFRGNVQTRL KL  
Sbjct: 176 DVRDAFICLTAATLAELPAGSVVGTASLRRKSQILHKYPALHVEENFRGNVQTRLSKLQG 235

Query: 352 GVVQATLLALAGLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDKMANYLASLNH 411
           G VQATLLALAGL+RL+MTENV SILS+DEMLPAVAQGAIGIACR+DDDKMA YLASLNH
Sbjct: 236 GKVQATLLALAGLKRLSMTENVASILSLDEMLPAVAQGAIGIACRTDDDKMATYLASLNH 295

Query: 412 EETRLAVVCERAFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGLY 471
           EETRLA+ CERAFLETLDGSCRTPIAGYAS+DE+GNCIF+GLVASPDGT+VLETSR+G Y
Sbjct: 296 EETRLAISCERAFLETLDGSCRTPIAGYASKDEEGNCIFRGLVASPDGTKVLETSRKGPY 355

Query: 472 AIDDMIAMGKDAGKELLSRAGPGFF 497
             +DM+ MGKDAG+ELLSRAGPGFF
Sbjct: 356 VYEDMVKMGKDAGQELLSRAGPGFF 380

[Close]

BLAST of Lag0031118 vs. ExPASy Swiss-Prot
Match: Q6H6D2 (Porphobilinogen deaminase, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=HEMC PE=2 SV=1)

HSP 1 Score: 473.0 bits (1216), Expect = 4.1e-132
Identity = 239/303 (78.88%), Postives = 264/303 (87.13%), Query Frame = 0


Query: 196 RDSPLALAQAHETRDKLMAIHPELAEDGAIQIVIIKTTGDKILSQPLADIGGKGLFTKEI 255
           R SPLALAQAHETRDKL A H ELAE+GA++IVIIKTTGD IL +PLADIGGKGLFTKEI
Sbjct: 55  RGSPLALAQAHETRDKLKAAHSELAEEGAVEIVIIKTTGDMILDKPLADIGGKGLFTKEI 114

Query: 256 DDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSAGSLAELPAGSVIG 315
           DDAL+ G IDIAVHSMKDVPTYLPE TILPCNLPREDVRDAFI L+A SLAELPAGSV+G
Sbjct: 115 DDALLQGRIDIAVHSMKDVPTYLPEGTILPCNLPREDVRDAFICLTASSLAELPAGSVVG 174

Query: 316 TASLRRKSQLLNRYPSLNVLENFRGNVQTRLRKLNEGVVQATLLALAGLRRLNMTENVTS 375
           +ASLRR+SQ+L +YPSL V+ NFRGNVQTRLRKL EG V ATLLALAGL+RLNM E  TS
Sbjct: 175 SASLRRQSQILYKYPSLKVV-NFRGNVQTRLRKLKEGDVHATLLALAGLKRLNMAETATS 234

Query: 376 ILSIDEMLPAVAQGAIGIACRSDDDKMANYLASLNHEETRLAVVCERAFLETLDGSCRTP 435
           +LS+DEMLPAVAQGAIGIACRS DD M NYL+SLNHE+TRLAV CER FL  LDG+CRTP
Sbjct: 235 VLSVDEMLPAVAQGAIGIACRSSDDTMMNYLSSLNHEDTRLAVACEREFLSVLDGNCRTP 294

Query: 436 IAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGLYAIDDMIAMGKDAGKELLSRAGPGF 495
           IA YASRD+DGNC F+GL+ASPDG+ V ETSR G Y  D M+ MGKDAG EL ++AGPGF
Sbjct: 295 IAAYASRDKDGNCSFRGLLASPDGSTVYETSRTGPYDFDIMVEMGKDAGHELKAKAGPGF 354

Query: 496 FDS 499
           FDS
Sbjct: 355 FDS 356

[Close]

BLAST of Lag0031118 vs. ExPASy Swiss-Prot
Match: Q3SVF3 (Porphobilinogen deaminase OS=Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255) OX=323098 GN=hemC PE=3 SV=1)

HSP 1 Score: 302.8 bits (774), Expect = 7.4e-81
Identity = 167/307 (54.40%), Postives = 206/307 (67.10%), Query Frame = 0


Query: 190 IQSASVRDSPLALAQAHETRDKLMAIHPELAEDGAIQIVIIKTTGDKILSQPLADIGGKG 249
           + +   R SPLALAQAHE RD+L   H    E   I I  I+T+GD I  +PL D+GGKG
Sbjct: 10  LATIGTRGSPLALAQAHEVRDRLARAHQVAPE--RIAIKTIRTSGDAIQDRPLFDVGGKG 69

Query: 250 LFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSAGSLAELP 309
           LFTKEI++AL+ G ID AVHS KDVPT+LP+ T LP  LPREDVRD FIS  AGSL +LP
Sbjct: 70  LFTKEIEEALLAGTIDFAVHSSKDVPTFLPDATWLPAFLPREDVRDVFISPHAGSLNDLP 129

Query: 310 AGSVIGTASLRRKSQLLNRYPSLNVLENFRGNVQTRLRKLNEGVVQATLLALAGLRRLNM 369
           AG+ +GTASLRR++ +L   P L V  + RGNV+TRLRK++ G   ATLLALAGL RL +
Sbjct: 130 AGATVGTASLRRQAMVLKLRPDLKV-NSLRGNVETRLRKISVGEADATLLALAGLNRLGL 189

Query: 370 TENVTSILSIDEMLPAVAQGAIGIACRSDDDKMANYLASLNHEETRLAVVCERAFLETLD 429
            +  T IL  DE LPAV QGAI I  R DDD++  ++ ++   ET +A+  ER+FL  LD
Sbjct: 190 QDKATRILETDEFLPAVGQGAIAIESRRDDDRINAFVKAIGDPETEVALSAERSFLALLD 249

Query: 430 GSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGLYAIDDMIAMGKDAGKELLS 489
           GSCRTPI G+   + D    F+GL+ SPDGT   ET+R G  A  D  A+G DA  EL  
Sbjct: 250 GSCRTPIGGHCRVNGD-RIDFRGLIISPDGTEFYETTREGARA--DAAALGADAAHELRE 309

Query: 490 RAGPGFF 497
           RAG  FF
Sbjct: 310 RAGEKFF 310

[Close]

BLAST of Lag0031118 vs. ExPASy Swiss-Prot
Match: A1B853 (Porphobilinogen deaminase OS=Paracoccus denitrificans (strain Pd 1222) OX=318586 GN=hemC PE=3 SV=1)

HSP 1 Score: 302.4 bits (773), Expect = 9.7e-81
Identity = 167/302 (55.30%), Postives = 212/302 (70.20%), Query Frame = 0


Query: 196 RDSPLALAQAHETRDKLMAIHPELAEDGAIQIVIIKTTGDKILSQPLADIGGKGLFTKEI 255
           R S LALAQAHETRD+LMA H  LA D A +IV+IKTTGD++L +PL +IGGKGLFT+EI
Sbjct: 16  RGSALALAQAHETRDRLMAAH-GLAAD-AFRIVVIKTTGDRVLDRPLKEIGGKGLFTREI 75

Query: 256 DDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSAGSLAELPAGSVIG 315
           +DAL+  +IDIAVHSMKD+PT  PE  ++ C LPREDVRDAF+S    +++ELP G+V+G
Sbjct: 76  EDALLAHEIDIAVHSMKDMPTIQPEGLVIDCYLPREDVRDAFVSAQFAAISELPQGAVVG 135

Query: 316 TASLRRKSQLLNRYPSLNVLENFRGNVQTRLRKLNEGVVQATLLALAGLRRLNMTENVTS 375
           ++SLRR++QL  R P L ++E FRGNVQTRL+KL +GV  AT LA+AGL RL M      
Sbjct: 136 SSSLRRRAQLAARRPDLKLVE-FRGNVQTRLKKLEDGVAVATFLAMAGLTRLGMLHVARG 195

Query: 376 ILSIDEMLPAVAQGAIGIACRSDDDKMANYLASLNHEETRLAVVCERAFLETLDGSCRTP 435
            +  DEMLPAVAQG IG+  R+DD + A+ LA+++  ++ L V  ERAFL  LDGSC+TP
Sbjct: 196 AVEPDEMLPAVAQGCIGVERRADDARTASLLAAISDRDSALRVTAERAFLARLDGSCQTP 255

Query: 436 IAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGLYAIDDMIAMGKDAGKELLSRAGPGF 495
           IAG A    D     +G +  PDG+ V+   R G  A  D  AMG D  +EL  RA   F
Sbjct: 256 IAGLAELQGD-RLRLRGEILRPDGSEVIAAERVGPAA--DGAAMGTDLAEELRGRAPADF 311

Query: 496 FD 498
           FD
Sbjct: 316 FD 311

[Close]

BLAST of Lag0031118 vs. ExPASy TrEMBL
Match: A0A6J1GCC7 (Hydroxymethylbilane synthase OS=Cucurbita moschata OX=3662 GN=LOC111452689 PE=3 SV=1)

HSP 1 Score: 641.0 bits (1652), Expect = 4.2e-180
Identity = 360/498 (72.29%), Postives = 362/498 (72.69%), Query Frame = 0


Query: 1   MGVLSSPSISHSPMAPPSNVRSLSLLGFSSLSLKPPAFSNGAKKFHGFPLIRASVAVEQQ 60
           MGV SSPSISH PMA P N+ SLS  GFSSLSLKPPAFS G KKFHGF LIRASVAVEQQ
Sbjct: 1   MGVFSSPSISHYPMARPCNLGSLSRHGFSSLSLKPPAFSTGGKKFHGFGLIRASVAVEQQ 60

Query: 61  TEKTKVALVRIGTRGSMLVMILVLWKLVCVTLAPLKEYFSCLDREVFLSRVVVAVFLMVV 120
            EKTKVALVRIGTRG                                             
Sbjct: 61  AEKTKVALVRIGTRG--------------------------------------------- 120

Query: 121 NQIIKNAKEWKMNQIKENNRFLRTNNGGESPLPPFVCLSSELIKFRKSIHVANGIDHGSV 180
                                                                       
Sbjct: 121 ------------------------------------------------------------ 180

Query: 181 VLTYNLNVRIQSASVRDSPLALAQAHETRDKLMAIHPELAEDGAIQIVIIKTTGDKILSQ 240
                            SPLALAQAHETR+KLMAIHPELAEDGAIQIVIIKTTGDKILSQ
Sbjct: 181 -----------------SPLALAQAHETREKLMAIHPELAEDGAIQIVIIKTTGDKILSQ 240

Query: 241 PLADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISL 300
           PLADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISL
Sbjct: 241 PLADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISL 300

Query: 301 SAGSLAELPAGSVIGTASLRRKSQLLNRYPSLNVLENFRGNVQTRLRKLNEGVVQATLLA 360
           S GSLAELPAGSVIGTASLRRKSQLLNRYPSLNVLENFRGNVQTRLRKLNEGVVQATLLA
Sbjct: 301 SVGSLAELPAGSVIGTASLRRKSQLLNRYPSLNVLENFRGNVQTRLRKLNEGVVQATLLA 360

Query: 361 LAGLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDKMANYLASLNHEETRLAVVC 420
           LAGLRRLNMTE VTSILSIDEMLPAVAQGAIGIACRSDDDKMANYLASLNHEETRLAVVC
Sbjct: 361 LAGLRRLNMTETVTSILSIDEMLPAVAQGAIGIACRSDDDKMANYLASLNHEETRLAVVC 376

Query: 421 ERAFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGLYAIDDMIAMG 480
           ERAFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRG YAIDDMIAMG
Sbjct: 421 ERAFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGPYAIDDMIAMG 376

Query: 481 KDAGKELLSRAGPGFFDS 499
           KDAGKELLSRAGPGFFDS
Sbjct: 481 KDAGKELLSRAGPGFFDS 376

[Close]

BLAST of Lag0031118 vs. ExPASy TrEMBL
Match: A0A6J1KA97 (Hydroxymethylbilane synthase OS=Cucurbita maxima OX=3661 GN=LOC111493083 PE=3 SV=1)

HSP 1 Score: 636.0 bits (1639), Expect = 1.4e-178
Identity = 357/498 (71.69%), Postives = 361/498 (72.49%), Query Frame = 0


Query: 1   MGVLSSPSISHSPMAPPSNVRSLSLLGFSSLSLKPPAFSNGAKKFHGFPLIRASVAVEQQ 60
           MGV SSPSISH PMA P N+ SLS  GFSSLSLKPPAFS G K+FHG  LIRASVAVEQQ
Sbjct: 1   MGVFSSPSISHYPMARPCNLGSLSRHGFSSLSLKPPAFSTGDKRFHGVGLIRASVAVEQQ 60

Query: 61  TEKTKVALVRIGTRGSMLVMILVLWKLVCVTLAPLKEYFSCLDREVFLSRVVVAVFLMVV 120
            EKTKVALVRIGTRG                                             
Sbjct: 61  AEKTKVALVRIGTRG--------------------------------------------- 120

Query: 121 NQIIKNAKEWKMNQIKENNRFLRTNNGGESPLPPFVCLSSELIKFRKSIHVANGIDHGSV 180
                                                                       
Sbjct: 121 ------------------------------------------------------------ 180

Query: 181 VLTYNLNVRIQSASVRDSPLALAQAHETRDKLMAIHPELAEDGAIQIVIIKTTGDKILSQ 240
                            SPLALAQAHETR+KLMAIHPELAEDGAIQIVIIKTTGDKILSQ
Sbjct: 181 -----------------SPLALAQAHETREKLMAIHPELAEDGAIQIVIIKTTGDKILSQ 240

Query: 241 PLADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISL 300
           PLADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISL
Sbjct: 241 PLADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISL 300

Query: 301 SAGSLAELPAGSVIGTASLRRKSQLLNRYPSLNVLENFRGNVQTRLRKLNEGVVQATLLA 360
           S GSLAELPAGSVIGTASLRRKSQLLNRYPSLNVLENFRGNVQTRLRKLNEGVVQATLLA
Sbjct: 301 SVGSLAELPAGSVIGTASLRRKSQLLNRYPSLNVLENFRGNVQTRLRKLNEGVVQATLLA 360

Query: 361 LAGLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDKMANYLASLNHEETRLAVVC 420
           LAGLRRLNMTE VTSILSIDEMLPAVAQGAIGIACRSDDDKMANYLASLNHEETRLAVVC
Sbjct: 361 LAGLRRLNMTETVTSILSIDEMLPAVAQGAIGIACRSDDDKMANYLASLNHEETRLAVVC 376

Query: 421 ERAFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGLYAIDDMIAMG 480
           ERAFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRG YAIDDM+AMG
Sbjct: 421 ERAFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGPYAIDDMVAMG 376

Query: 481 KDAGKELLSRAGPGFFDS 499
           KDAGKELLSRAGPGFFDS
Sbjct: 481 KDAGKELLSRAGPGFFDS 376

[Close]

BLAST of Lag0031118 vs. ExPASy TrEMBL
Match: A0A6J1IZW2 (Hydroxymethylbilane synthase OS=Cucurbita maxima OX=3661 GN=LOC111481450 PE=3 SV=1)

HSP 1 Score: 633.6 bits (1633), Expect = 6.8e-178
Identity = 354/498 (71.08%), Postives = 362/498 (72.69%), Query Frame = 0


Query: 1   MGVLSSPSISHSPMAPPSNVRSLSLLGFSSLSLKPPAFSNGAKKFHGFPLIRASVAVEQQ 60
           M  LSSPS+S+SPMA P N+ SLSL GFSSLSLKPPAF+N  KKFHGF LIRASVAVEQQ
Sbjct: 1   MVALSSPSVSNSPMARPCNLASLSLPGFSSLSLKPPAFTNAPKKFHGFGLIRASVAVEQQ 60

Query: 61  TEKTKVALVRIGTRGSMLVMILVLWKLVCVTLAPLKEYFSCLDREVFLSRVVVAVFLMVV 120
            EKTKVAL+RIGTRG                                             
Sbjct: 61  PEKTKVALLRIGTRG--------------------------------------------- 120

Query: 121 NQIIKNAKEWKMNQIKENNRFLRTNNGGESPLPPFVCLSSELIKFRKSIHVANGIDHGSV 180
                                                                       
Sbjct: 121 ------------------------------------------------------------ 180

Query: 181 VLTYNLNVRIQSASVRDSPLALAQAHETRDKLMAIHPELAEDGAIQIVIIKTTGDKILSQ 240
                            SPLALAQAHETRDKLMA HPELAEDGAIQIV+IKTTGDKILSQ
Sbjct: 181 -----------------SPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQ 240

Query: 241 PLADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISL 300
           PLADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISL
Sbjct: 241 PLADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISL 300

Query: 301 SAGSLAELPAGSVIGTASLRRKSQLLNRYPSLNVLENFRGNVQTRLRKLNEGVVQATLLA 360
            AGSLAELPAGSV+GTASLRRKSQLLNRYPSL VLENFRGNVQTRL+KLNEGVVQATLLA
Sbjct: 301 RAGSLAELPAGSVVGTASLRRKSQLLNRYPSLEVLENFRGNVQTRLKKLNEGVVQATLLA 360

Query: 361 LAGLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDKMANYLASLNHEETRLAVVC 420
           LAGLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDKMANYLASLNHEETRLAVVC
Sbjct: 361 LAGLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDKMANYLASLNHEETRLAVVC 376

Query: 421 ERAFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGLYAIDDMIAMG 480
           ERAFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRG YAIDDMIAMG
Sbjct: 421 ERAFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGSYAIDDMIAMG 376

Query: 481 KDAGKELLSRAGPGFFDS 499
           KDAGKELLSRAGPGFFDS
Sbjct: 481 KDAGKELLSRAGPGFFDS 376

[Close]

BLAST of Lag0031118 vs. ExPASy TrEMBL
Match: A0A6J1DII9 (Hydroxymethylbilane synthase OS=Momordica charantia OX=3673 GN=LOC111020357 PE=3 SV=1)

HSP 1 Score: 632.1 bits (1629), Expect = 2.0e-177
Identity = 353/498 (70.88%), Postives = 361/498 (72.49%), Query Frame = 0


Query: 1   MGVLSSPSISHSPMAPPSNVRSLSLLGFSSLSLKPPAFSNGAKKFHGFPLIRASVAVEQQ 60
           MGV SSP I+HSPMA P NV SLS LGFSSLSLKPPAF NG KKF G  L+RASVAVEQQ
Sbjct: 1   MGVFSSPPITHSPMARPCNVGSLSPLGFSSLSLKPPAFCNGGKKFGGLGLVRASVAVEQQ 60

Query: 61  TEKTKVALVRIGTRGSMLVMILVLWKLVCVTLAPLKEYFSCLDREVFLSRVVVAVFLMVV 120
           TEKTKVALVRIGTRG                                             
Sbjct: 61  TEKTKVALVRIGTRG--------------------------------------------- 120

Query: 121 NQIIKNAKEWKMNQIKENNRFLRTNNGGESPLPPFVCLSSELIKFRKSIHVANGIDHGSV 180
                                                                       
Sbjct: 121 ------------------------------------------------------------ 180

Query: 181 VLTYNLNVRIQSASVRDSPLALAQAHETRDKLMAIHPELAEDGAIQIVIIKTTGDKILSQ 240
                            SPLALAQA+ETRDKLMAIHPELAEDGAIQI+IIKTTGDKILSQ
Sbjct: 181 -----------------SPLALAQAYETRDKLMAIHPELAEDGAIQIIIIKTTGDKILSQ 240

Query: 241 PLADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISL 300
           PLADIGGKGLFTKEIDDALIN DIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISL
Sbjct: 241 PLADIGGKGLFTKEIDDALINSDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISL 300

Query: 301 SAGSLAELPAGSVIGTASLRRKSQLLNRYPSLNVLENFRGNVQTRLRKLNEGVVQATLLA 360
           SAGSLAELPAGSVIGTASLRRKSQLLNRYPSL VLENFRGNVQTRLRKLNEGVVQATLLA
Sbjct: 301 SAGSLAELPAGSVIGTASLRRKSQLLNRYPSLKVLENFRGNVQTRLRKLNEGVVQATLLA 360

Query: 361 LAGLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDKMANYLASLNHEETRLAVVC 420
           LAGLRRLNMTENVTS+LSIDEMLPAVAQGAIGIACRS+DDKMANYLASLNHEETRLAVVC
Sbjct: 361 LAGLRRLNMTENVTSVLSIDEMLPAVAQGAIGIACRSNDDKMANYLASLNHEETRLAVVC 376

Query: 421 ERAFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGLYAIDDMIAMG 480
           ERAFLE LDGSCRTPIAGYAS+DEDGNCIFKGLVASPDGTRVLETSRRG YAIDDM+AMG
Sbjct: 421 ERAFLEMLDGSCRTPIAGYASKDEDGNCIFKGLVASPDGTRVLETSRRGAYAIDDMVAMG 376

Query: 481 KDAGKELLSRAGPGFFDS 499
           KDAGKELLSRAGPGFFDS
Sbjct: 481 KDAGKELLSRAGPGFFDS 376

[Close]

BLAST of Lag0031118 vs. ExPASy TrEMBL
Match: A0A6J1F354 (Hydroxymethylbilane synthase OS=Cucurbita moschata OX=3662 GN=LOC111441758 PE=3 SV=1)

HSP 1 Score: 631.7 bits (1628), Expect = 2.6e-177
Identity = 353/498 (70.88%), Postives = 360/498 (72.29%), Query Frame = 0


Query: 1   MGVLSSPSISHSPMAPPSNVRSLSLLGFSSLSLKPPAFSNGAKKFHGFPLIRASVAVEQQ 60
           M  LSSPS+SHSPMA P N+ SLSL GFSSLSLKPPAF+NG KK HGF LIRASVAVEQQ
Sbjct: 1   MVALSSPSVSHSPMAHPCNLASLSLPGFSSLSLKPPAFTNGPKKLHGFGLIRASVAVEQQ 60

Query: 61  TEKTKVALVRIGTRGSMLVMILVLWKLVCVTLAPLKEYFSCLDREVFLSRVVVAVFLMVV 120
             KTKVAL+RIGTRG                                             
Sbjct: 61  AGKTKVALLRIGTRG--------------------------------------------- 120

Query: 121 NQIIKNAKEWKMNQIKENNRFLRTNNGGESPLPPFVCLSSELIKFRKSIHVANGIDHGSV 180
                                                                       
Sbjct: 121 ------------------------------------------------------------ 180

Query: 181 VLTYNLNVRIQSASVRDSPLALAQAHETRDKLMAIHPELAEDGAIQIVIIKTTGDKILSQ 240
                            SPLALAQAHETRDKLMA HPELAEDGAIQIV+IKTTGDKILSQ
Sbjct: 181 -----------------SPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQ 240

Query: 241 PLADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISL 300
           PLADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISL
Sbjct: 241 PLADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISL 300

Query: 301 SAGSLAELPAGSVIGTASLRRKSQLLNRYPSLNVLENFRGNVQTRLRKLNEGVVQATLLA 360
            AGSLAELPAGSV+GTASLRRKSQLLNRYPSL VLENFRGNVQTRL+KLNEGVVQATLLA
Sbjct: 301 RAGSLAELPAGSVVGTASLRRKSQLLNRYPSLEVLENFRGNVQTRLKKLNEGVVQATLLA 360

Query: 361 LAGLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDKMANYLASLNHEETRLAVVC 420
           LAGLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDKMA YLASLNHEETRLAVVC
Sbjct: 361 LAGLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDKMAEYLASLNHEETRLAVVC 376

Query: 421 ERAFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGLYAIDDMIAMG 480
           ERAFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRG YAIDDMIAMG
Sbjct: 421 ERAFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGSYAIDDMIAMG 376

Query: 481 KDAGKELLSRAGPGFFDS 499
           KDAGKELLSRAGPGFFDS
Sbjct: 481 KDAGKELLSRAGPGFFDS 376

[Close]

BLAST of Lag0031118 vs. TAIR 10
Match: AT5G08280.1 (hydroxymethylbilane synthase )

HSP 1 Score: 510.0 bits (1312), Expect = 2.2e-144
Identity = 258/325 (79.38%), Postives = 285/325 (87.69%), Query Frame = 0


Query: 172 ANGIDHGSVVLTYNLNVRIQSASVRDSPLALAQAHETRDKLMAIHPELAEDGAIQIVIIK 231
           ++G     V +       I     R SPLALAQA+ETR+KL   HPEL EDGAI I IIK
Sbjct: 56  SSGFVKACVAVEQKTRTAIIRIGTRGSPLALAQAYETREKLKKKHPELVEDGAIHIEIIK 115

Query: 232 TTGDKILSQPLADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPRE 291
           TTGDKILSQPLADIGGKGLFTKEID+ALING IDIAVHSMKDVPTYLPEKTILPCNLPRE
Sbjct: 116 TTGDKILSQPLADIGGKGLFTKEIDEALINGHIDIAVHSMKDVPTYLPEKTILPCNLPRE 175

Query: 292 DVRDAFISLSAGSLAELPAGSVIGTASLRRKSQLLNRYPSLNVLENFRGNVQTRLRKLNE 351
           DVRDAFI L+A +LAELPAGSV+GTASLRRKSQ+L++YP+L+V ENFRGNVQTRL KL  
Sbjct: 176 DVRDAFICLTAATLAELPAGSVVGTASLRRKSQILHKYPALHVEENFRGNVQTRLSKLQG 235

Query: 352 GVVQATLLALAGLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDKMANYLASLNH 411
           G VQATLLALAGL+RL+MTENV SILS+DEMLPAVAQGAIGIACR+DDDKMA YLASLNH
Sbjct: 236 GKVQATLLALAGLKRLSMTENVASILSLDEMLPAVAQGAIGIACRTDDDKMATYLASLNH 295

Query: 412 EETRLAVVCERAFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGLY 471
           EETRLA+ CERAFLETLDGSCRTPIAGYAS+DE+GNCIF+GLVASPDGT+VLETSR+G Y
Sbjct: 296 EETRLAISCERAFLETLDGSCRTPIAGYASKDEEGNCIFRGLVASPDGTKVLETSRKGPY 355

Query: 472 AIDDMIAMGKDAGKELLSRAGPGFF 497
             +DM+ MGKDAG+ELLSRAGPGFF
Sbjct: 356 VYEDMVKMGKDAGQELLSRAGPGFF 380

The following BLAST results are available for this feature:

BLAST of Lag0031118 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038906286.1	1.7e-183	73.09	porphobilinogen deaminase, chloroplastic [Benincasa hispida]	[more]
XP_023523547.1	3.0e-180	72.49	porphobilinogen deaminase, chloroplastic-like [Cucurbita pepo subsp. pepo]	[more]
XP_022949290.1	8.7e-180	72.29	porphobilinogen deaminase, chloroplastic-like [Cucurbita moschata]	[more]
KAG7036601.1	1.9e-179	72.09	Porphobilinogen deaminase, chloroplastic [Cucurbita argyrosperma subsp. argyrosp...	[more]
KAG6606898.1	5.7e-179	71.89	Porphobilinogen deaminase, chloroplastic, partial [Cucurbita argyrosperma subsp....	[more]

BLAST of Lag0031118 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q43082	6.3e-149	88.12	Porphobilinogen deaminase, chloroplastic OS=Pisum sativum OX=3888 GN=HEMC PE=1 S...	[more]
Q43316	3.0e-143	79.38	Porphobilinogen deaminase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=HEMC...	[more]
Q6H6D2	4.1e-132	78.88	Porphobilinogen deaminase, chloroplastic OS=Oryza sativa subsp. japonica OX=3994...	[more]
Q3SVF3	7.4e-81	54.40	Porphobilinogen deaminase OS=Nitrobacter winogradskyi (strain ATCC 25391 / DSM 1...	[more]
A1B853	9.7e-81	55.30	Porphobilinogen deaminase OS=Paracoccus denitrificans (strain Pd 1222) OX=318586...	[more]

BLAST of Lag0031118 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GCC7	4.2e-180	72.29	Hydroxymethylbilane synthase OS=Cucurbita moschata OX=3662 GN=LOC111452689 PE=3 ...	[more]
A0A6J1KA97	1.4e-178	71.69	Hydroxymethylbilane synthase OS=Cucurbita maxima OX=3661 GN=LOC111493083 PE=3 SV...	[more]
A0A6J1IZW2	6.8e-178	71.08	Hydroxymethylbilane synthase OS=Cucurbita maxima OX=3661 GN=LOC111481450 PE=3 SV...	[more]
A0A6J1DII9	2.0e-177	70.88	Hydroxymethylbilane synthase OS=Momordica charantia OX=3673 GN=LOC111020357 PE=3...	[more]
A0A6J1F354	2.6e-177	70.88	Hydroxymethylbilane synthase OS=Cucurbita moschata OX=3662 GN=LOC111441758 PE=3 ...	[more]

BLAST of Lag0031118 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G08280.1	2.2e-144	79.38	hydroxymethylbilane synthase	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000860	Porphobilinogen deaminase	PRINTS	PR00151	PORPHBDMNASE	coord: 333..350 score: 53.97 coord: 313..330 score: 56.08 coord: 234..254 score: 66.21 coord: 265..284 score: 61.19 coord: 420..437 score: 53.17
IPR000860	Porphobilinogen deaminase	TIGRFAM	TIGR00212	TIGR00212	coord: 195..489 e-value: 3.4E-97 score: 323.0
IPR000860	Porphobilinogen deaminase	PANTHER	PTHR11557	PORPHOBILINOGEN DEAMINASE	coord: 171..497 coord: 15..80
IPR000860	Porphobilinogen deaminase	HAMAP	MF_00260	Porphobil_deam	coord: 190..490 score: 33.19875
None	No IPR available	GENE3D	3.40.190.10		coord: 196..400 e-value: 3.0E-78 score: 263.9
None	No IPR available	GENE3D	3.40.190.10		coord: 292..392 e-value: 3.0E-78 score: 263.9
None	No IPR available	PANTHER	PTHR11557:SF8	BNAC02G01240D PROTEIN	coord: 171..497
None	No IPR available	PANTHER	PTHR11557:SF8	BNAC02G01240D PROTEIN	coord: 15..80
None	No IPR available	CDD	cd13648	PBP2_PBGD_1	coord: 196..469 e-value: 0.0 score: 510.031
None	No IPR available	SUPERFAMILY	53850	Periplasmic binding protein-like II	coord: 194..408
IPR036803	Porphobilinogen deaminase, C-terminal domain superfamily	GENE3D	3.30.160.40		coord: 411..497 e-value: 3.2E-23 score: 83.9
IPR036803	Porphobilinogen deaminase, C-terminal domain superfamily	SUPERFAMILY	54782	Porphobilinogen deaminase (hydroxymethylbilane synthase), C-terminal domain	coord: 410..497
IPR022417	Porphobilinogen deaminase, N-terminal	PFAM	PF01379	Porphobil_deam	coord: 196..403 e-value: 1.1E-72 score: 243.9
IPR022418	Porphobilinogen deaminase, C-terminal	PFAM	PF03900	Porphobil_deamC	coord: 416..488 e-value: 3.7E-15 score: 56.1
IPR022419	Porphobilinogen deaminase, dipyrromethane cofactor binding site	PROSITE	PS00533	PORPHOBILINOGEN_DEAM	coord: 421..437

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0031118.1	Lag0031118.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0018160	peptidyl-pyrromethane cofactor linkage
biological_process	GO:0006782	protoporphyrinogen IX biosynthetic process
biological_process	GO:0017157	regulation of exocytosis
biological_process	GO:0033014	tetrapyrrole biosynthetic process
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0005886	plasma membrane
molecular_function	GO:0005096	GTPase activator activity
molecular_function	GO:0004418	hydroxymethylbilane synthase activity
molecular_function	GO:0045159	myosin II binding
molecular_function	GO:0019905	syntaxin binding