Lag0031066 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0031066 vs. NCBI nr
Match: XP_023523223.1 (long chain acyl-CoA synthetase 1 [Cucurbita pepo subsp. pepo] >XP_023523224.1 long chain acyl-CoA synthetase 1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1256.9 bits (3251), Expect = 0.0e+00
Identity = 601/659 (91.20%), Postives = 639/659 (96.97%), Query Frame = 0

Query: 1   MKSFSIKVEEGREGKDGKPSVGPVYRNVLSKDGFPPLYEDLSTSWDLFSVSAKKYAEKQM 60
           MKSFSIKVEEG+EGKDGKPSVGPVYRNVL+KDGFPPLYEDLST+WD+FSVSAKKYA K+M
Sbjct: 1   MKSFSIKVEEGKEGKDGKPSVGPVYRNVLTKDGFPPLYEDLSTTWDIFSVSAKKYAGKRM 60

Query: 61  LGWRKFVEGKPGPYIWKTYKEVYVDVLNIGSALRAAGAEPGSRIGIYGANCPQWIIAMEA 120
           LGWRKFVEGKPGPYIWKTY+EVYVDVLNIGSALRA GA+ GSRIGIYGANCPQWIIAMEA
Sbjct: 61  LGWRKFVEGKPGPYIWKTYQEVYVDVLNIGSALRAVGAKAGSRIGIYGANCPQWIIAMEA 120

Query: 121 CNAHSLICVPLYDTLGHGAVNFIIDHAEIDIVFVQDKKAKELLNSESTNPKRLKVVVCFT 180
           CNAHSL+ VPLYDTLGHGAVN+II+HAEIDI+F+QDKKAKELL+S STNPKRLKV++CFT
Sbjct: 121 CNAHSLVAVPLYDTLGHGAVNYIIEHAEIDIIFIQDKKAKELLSSGSTNPKRLKVIICFT 180

Query: 181 SLKGEEKTSAVGNGMTPYSWDEFLRLGKENPSEITPPKPLDICTIMYTSGTSGTPKGVVL 240
           SLKGEEKTSAVGNG+ PYSWDEFLRLGKE+P EI PP+PLD+CTIMYTSGTSGTPKGVVL
Sbjct: 181 SLKGEEKTSAVGNGIKPYSWDEFLRLGKEDPFEIVPPRPLDVCTIMYTSGTSGTPKGVVL 240

Query: 241 THETVTTFIRGVDIYLEQFEDKMTEDDVYLSFLPLAHILDRTVEEYFFRKGASVGYYHGD 300
           THET+TTFIRGVD+++EQFEDKMTEDDVYLSFLPLAHILDRTVEEYFFR GASVGYYHGD
Sbjct: 241 THETITTFIRGVDVFMEQFEDKMTEDDVYLSFLPLAHILDRTVEEYFFRNGASVGYYHGD 300

Query: 301 LNAIKDDLMELKPTLLVGVPRVFERIHEGIKQAVQILNPVRRKAFDLLYKYKLSWLNSGY 360
           L+AIKDDLMELKPTLLVGVPRVFE+IHEGIK+AVQ+LNPVRRKAFDLLYKYKLSW+NSGY
Sbjct: 301 LDAIKDDLMELKPTLLVGVPRVFEKIHEGIKEAVQVLNPVRRKAFDLLYKYKLSWMNSGY 360

Query: 361 KQKYASPLADFLAFRKVKARLGGRLRLIISGGAALSSEVEEFLRVTCCAFFVQGYGLTET 420
           KQK ASPLADFLAFRKVKARLGGRLRLIISGGAALS+EVEEFLRVTCCA FVQGYGLTET
Sbjct: 361 KQKDASPLADFLAFRKVKARLGGRLRLIISGGAALSAEVEEFLRVTCCALFVQGYGLTET 420

Query: 421 CGPTTIGFPDEMCMLGTVGAVTLYNELRLEEVPEMGYNPLGERPCGEICVRGKTVSNEYY 480
           CGPTT+GFPDEMCMLG VGAVTLYNELRLEEVPEMGY+PLG+RPCGEICVRGKTV NEYY
Sbjct: 421 CGPTTVGFPDEMCMLGNVGAVTLYNELRLEEVPEMGYDPLGDRPCGEICVRGKTVFNEYY 480

Query: 481 KNPELTKEAIKDGWFHTGDIGEILPNGVLKIIDRKKNLIKLSQGEYIALEYLENVYSITP 540
           KNPELTKE+IKDGWFHTGDIGEI PNGV+KIIDRKKNLIKLSQGEYIALEYLENVYS+TP
Sbjct: 481 KNPELTKESIKDGWFHTGDIGEIFPNGVVKIIDRKKNLIKLSQGEYIALEYLENVYSVTP 540

Query: 541 IVEDIWIFGNSFKSMLVAVVVLHEENTKKWANSNGFLCSFSELCSLEQLRHYVLSELTST 600
           IVEDIWI+GNSFKS LVAVVVLHEENTKKWANSNGFLCSFSELC LEQLR YVLSELTST
Sbjct: 541 IVEDIWIYGNSFKSKLVAVVVLHEENTKKWANSNGFLCSFSELCCLEQLRQYVLSELTST 600

Query: 601 AERNKLKRFEYIKGVVLESRPFDIEDDLVTATLKKKRNKLLKHYEEEINAEYKNLEANR 660
           AER KLKRFEYIKGVVLESRPFDIED+LVTATLKKKRN LLKHYE+EINAEYKNLEA R
Sbjct: 601 AERTKLKRFEYIKGVVLESRPFDIEDELVTATLKKKRNNLLKHYEKEINAEYKNLEAKR 659

BLAST of Lag0031066 vs. NCBI nr
Match: KAG6606924.1 (Long chain acyl-CoA synthetase 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1255.7 bits (3248), Expect = 0.0e+00
Identity = 600/659 (91.05%), Postives = 639/659 (96.97%), Query Frame = 0

Query: 1   MKSFSIKVEEGREGKDGKPSVGPVYRNVLSKDGFPPLYEDLSTSWDLFSVSAKKYAEKQM 60
           MKSFSIKVEEG+EGKDGKPSVGPVYRNVL+KDGFPPLYEDLST+WD+FSVSAKKYA K+M
Sbjct: 1   MKSFSIKVEEGKEGKDGKPSVGPVYRNVLTKDGFPPLYEDLSTTWDIFSVSAKKYAGKRM 60

Query: 61  LGWRKFVEGKPGPYIWKTYKEVYVDVLNIGSALRAAGAEPGSRIGIYGANCPQWIIAMEA 120
           LGWRKFVEGKPGPYIWKTY+EVYVDVLNIGSALRA GA+ GSRIGIYGANCPQWIIAMEA
Sbjct: 61  LGWRKFVEGKPGPYIWKTYQEVYVDVLNIGSALRAVGAKAGSRIGIYGANCPQWIIAMEA 120

Query: 121 CNAHSLICVPLYDTLGHGAVNFIIDHAEIDIVFVQDKKAKELLNSESTNPKRLKVVVCFT 180
           CNAHSL+ VPLYDTLGHGAVN+II+HAEIDI+F+QDKKAKELL+S STNPKRLKV++CFT
Sbjct: 121 CNAHSLVAVPLYDTLGHGAVNYIIEHAEIDIIFIQDKKAKELLSSGSTNPKRLKVIICFT 180

Query: 181 SLKGEEKTSAVGNGMTPYSWDEFLRLGKENPSEITPPKPLDICTIMYTSGTSGTPKGVVL 240
           SLKGEEKTSAVGNG+ PYSWDEFLRLGKE+P EI PP+PLD+CTIMYTSGTSGTPKGVVL
Sbjct: 181 SLKGEEKTSAVGNGIKPYSWDEFLRLGKEDPFEIVPPRPLDVCTIMYTSGTSGTPKGVVL 240

Query: 241 THETVTTFIRGVDIYLEQFEDKMTEDDVYLSFLPLAHILDRTVEEYFFRKGASVGYYHGD 300
           THET+TTFIRGVD+++EQFEDKMTEDDVYLSFLPLAHILDRTVEEYFFR GASVGYYHGD
Sbjct: 241 THETITTFIRGVDVFMEQFEDKMTEDDVYLSFLPLAHILDRTVEEYFFRNGASVGYYHGD 300

Query: 301 LNAIKDDLMELKPTLLVGVPRVFERIHEGIKQAVQILNPVRRKAFDLLYKYKLSWLNSGY 360
           L+AIKDDLMELKPTLLVGVPRVFE+IHEGIK+AVQ+LNPVRRKAFDLLYKYKLSW+NSGY
Sbjct: 301 LDAIKDDLMELKPTLLVGVPRVFEKIHEGIKEAVQVLNPVRRKAFDLLYKYKLSWMNSGY 360

Query: 361 KQKYASPLADFLAFRKVKARLGGRLRLIISGGAALSSEVEEFLRVTCCAFFVQGYGLTET 420
           KQK ASPLADFLAFRKVKARLGGRLRLIISGGAALS+EVEEFLRVTCCA FVQGYGLTET
Sbjct: 361 KQKDASPLADFLAFRKVKARLGGRLRLIISGGAALSAEVEEFLRVTCCALFVQGYGLTET 420

Query: 421 CGPTTIGFPDEMCMLGTVGAVTLYNELRLEEVPEMGYNPLGERPCGEICVRGKTVSNEYY 480
           CGPTT+GFPDEMCMLG VGAVTLYNELRLEEVPEMGY+PLG+RPCGEICVRGKTV NEYY
Sbjct: 421 CGPTTVGFPDEMCMLGNVGAVTLYNELRLEEVPEMGYDPLGDRPCGEICVRGKTVFNEYY 480

Query: 481 KNPELTKEAIKDGWFHTGDIGEILPNGVLKIIDRKKNLIKLSQGEYIALEYLENVYSITP 540
           KNPELTKE+IKDGWFHTGDIGEI PNGV+KIIDRKKNLIKLSQGEYIALEYLENVYS+TP
Sbjct: 481 KNPELTKESIKDGWFHTGDIGEIFPNGVVKIIDRKKNLIKLSQGEYIALEYLENVYSVTP 540

Query: 541 IVEDIWIFGNSFKSMLVAVVVLHEENTKKWANSNGFLCSFSELCSLEQLRHYVLSELTST 600
           IVEDIWI+GNSFKS LVAVVVLHEENTKKWANSNGFLCSFSELC LEQLR YVLSELTST
Sbjct: 541 IVEDIWIYGNSFKSKLVAVVVLHEENTKKWANSNGFLCSFSELCCLEQLRQYVLSELTST 600

Query: 601 AERNKLKRFEYIKGVVLESRPFDIEDDLVTATLKKKRNKLLKHYEEEINAEYKNLEANR 660
           AER KLKRFEYIKGVVLESRPFDIED+LVTATLKKKRN L+KHYE+EINAEYKNLEA R
Sbjct: 601 AERTKLKRFEYIKGVVLESRPFDIEDELVTATLKKKRNNLVKHYEKEINAEYKNLEAKR 659

BLAST of Lag0031066 vs. NCBI nr
Match: XP_022998412.1 (long chain acyl-CoA synthetase 1-like [Cucurbita maxima])

HSP 1 Score: 1254.6 bits (3245), Expect = 0.0e+00
Identity = 600/659 (91.05%), Postives = 637/659 (96.66%), Query Frame = 0

Query: 1   MKSFSIKVEEGREGKDGKPSVGPVYRNVLSKDGFPPLYEDLSTSWDLFSVSAKKYAEKQM 60
           MKSFSIKVEEG EGKDGKPSVGPVYRNVL+KDGFPPLYEDLST+WD+FSVSAKKYA K+M
Sbjct: 1   MKSFSIKVEEGTEGKDGKPSVGPVYRNVLTKDGFPPLYEDLSTTWDIFSVSAKKYAGKRM 60

Query: 61  LGWRKFVEGKPGPYIWKTYKEVYVDVLNIGSALRAAGAEPGSRIGIYGANCPQWIIAMEA 120
           LGWRKFVEGKPGPYIWKTY+EVYVDVLNIGSALRA GA+ GSRIGIYGANCPQWIIAMEA
Sbjct: 61  LGWRKFVEGKPGPYIWKTYQEVYVDVLNIGSALRAVGAKAGSRIGIYGANCPQWIIAMEA 120

Query: 121 CNAHSLICVPLYDTLGHGAVNFIIDHAEIDIVFVQDKKAKELLNSESTNPKRLKVVVCFT 180
           CNAHSL+ VPLYDTLGHGAVN+II+HAEIDI+F+QDKKAKELL+SESTNPKRLKV++CFT
Sbjct: 121 CNAHSLVAVPLYDTLGHGAVNYIIEHAEIDIIFIQDKKAKELLSSESTNPKRLKVIICFT 180

Query: 181 SLKGEEKTSAVGNGMTPYSWDEFLRLGKENPSEITPPKPLDICTIMYTSGTSGTPKGVVL 240
           SLKGEEK +AVGNG+ PYSWDEFLRLGKE+P EI PP+PLD+CTIMYTSGTSGTPKGVVL
Sbjct: 181 SLKGEEKAAAVGNGIKPYSWDEFLRLGKEDPFEIVPPRPLDVCTIMYTSGTSGTPKGVVL 240

Query: 241 THETVTTFIRGVDIYLEQFEDKMTEDDVYLSFLPLAHILDRTVEEYFFRKGASVGYYHGD 300
           THET+TTFIRGVD+++EQFEDKMTEDDVYLSFLPLAHILDRTVEEYFFR GASVGYYHGD
Sbjct: 241 THETITTFIRGVDVFMEQFEDKMTEDDVYLSFLPLAHILDRTVEEYFFRNGASVGYYHGD 300

Query: 301 LNAIKDDLMELKPTLLVGVPRVFERIHEGIKQAVQILNPVRRKAFDLLYKYKLSWLNSGY 360
           L+AIKDDLMELKPTLLVGVPRVFE+IHEGIK+AVQ+LNPVRRKAFDLLYKYKLSW+NSGY
Sbjct: 301 LDAIKDDLMELKPTLLVGVPRVFEKIHEGIKEAVQVLNPVRRKAFDLLYKYKLSWMNSGY 360

Query: 361 KQKYASPLADFLAFRKVKARLGGRLRLIISGGAALSSEVEEFLRVTCCAFFVQGYGLTET 420
           KQK ASPLADFLAFRKVKARLGGRLRLIISGGAALS+EVEEFLRVTCCA FVQGYGLTET
Sbjct: 361 KQKDASPLADFLAFRKVKARLGGRLRLIISGGAALSAEVEEFLRVTCCALFVQGYGLTET 420

Query: 421 CGPTTIGFPDEMCMLGTVGAVTLYNELRLEEVPEMGYNPLGERPCGEICVRGKTVSNEYY 480
           CGPTT+GFPDEMCMLG VGAVTLYNELRLEEVPEMGYNPLG+RPCGEICVRGKTV NEYY
Sbjct: 421 CGPTTVGFPDEMCMLGKVGAVTLYNELRLEEVPEMGYNPLGDRPCGEICVRGKTVFNEYY 480

Query: 481 KNPELTKEAIKDGWFHTGDIGEILPNGVLKIIDRKKNLIKLSQGEYIALEYLENVYSITP 540
           KNPELTKE+IKDGWFHTGDIGEI PNGV+KIIDRKKNLIKLSQGEYIALEYLENVYS+TP
Sbjct: 481 KNPELTKESIKDGWFHTGDIGEIFPNGVVKIIDRKKNLIKLSQGEYIALEYLENVYSVTP 540

Query: 541 IVEDIWIFGNSFKSMLVAVVVLHEENTKKWANSNGFLCSFSELCSLEQLRHYVLSELTST 600
           IVEDIWI+GNSFKS LVAVVVLHEENTKKWANSNGFLCSFSELC LEQLR YVL ELTST
Sbjct: 541 IVEDIWIYGNSFKSKLVAVVVLHEENTKKWANSNGFLCSFSELCCLEQLRQYVLFELTST 600

Query: 601 AERNKLKRFEYIKGVVLESRPFDIEDDLVTATLKKKRNKLLKHYEEEINAEYKNLEANR 660
           AER KLKRFEYIKGVVLESRPFDIED+LVTATLKKKRN LLKHYE+EINAEYKNLEA R
Sbjct: 601 AERTKLKRFEYIKGVVLESRPFDIEDELVTATLKKKRNNLLKHYEKEINAEYKNLEAKR 659

BLAST of Lag0031066 vs. NCBI nr
Match: KAG7036628.1 (Long chain acyl-CoA synthetase 1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1254.2 bits (3244), Expect = 0.0e+00
Identity = 599/659 (90.90%), Postives = 639/659 (96.97%), Query Frame = 0

Query: 1   MKSFSIKVEEGREGKDGKPSVGPVYRNVLSKDGFPPLYEDLSTSWDLFSVSAKKYAEKQM 60
           MKSFSIKVEEG+EGKDGKPSVGPVYRNVL+KDGFPPLYEDLST+WD+FSVSAKKYA K+M
Sbjct: 1   MKSFSIKVEEGKEGKDGKPSVGPVYRNVLTKDGFPPLYEDLSTTWDIFSVSAKKYAGKRM 60

Query: 61  LGWRKFVEGKPGPYIWKTYKEVYVDVLNIGSALRAAGAEPGSRIGIYGANCPQWIIAMEA 120
           LGWRKFVEG+PGPYIWKTY+EVYVDVLNIGSALRA GA+ GSRIGIYGANCPQWIIAMEA
Sbjct: 61  LGWRKFVEGEPGPYIWKTYQEVYVDVLNIGSALRAVGAKAGSRIGIYGANCPQWIIAMEA 120

Query: 121 CNAHSLICVPLYDTLGHGAVNFIIDHAEIDIVFVQDKKAKELLNSESTNPKRLKVVVCFT 180
           CNAHSL+ VPLYDTLGHGAVN+II+HAEIDI+F+QDKKAKELL+S STNPKRLKV++CFT
Sbjct: 121 CNAHSLVAVPLYDTLGHGAVNYIIEHAEIDIIFIQDKKAKELLSSGSTNPKRLKVIICFT 180

Query: 181 SLKGEEKTSAVGNGMTPYSWDEFLRLGKENPSEITPPKPLDICTIMYTSGTSGTPKGVVL 240
           SLKGEEKTSAVGNG+ PYSWDEFLRLGKE+P EI PP+PLD+CTIMYTSGTSGTPKGVVL
Sbjct: 181 SLKGEEKTSAVGNGIKPYSWDEFLRLGKEDPFEIVPPRPLDVCTIMYTSGTSGTPKGVVL 240

Query: 241 THETVTTFIRGVDIYLEQFEDKMTEDDVYLSFLPLAHILDRTVEEYFFRKGASVGYYHGD 300
           THET+TTFIRGVD+++EQFEDKMTEDDVYLSFLPLAHILDRTVEEYFFR GASVGYYHGD
Sbjct: 241 THETITTFIRGVDVFMEQFEDKMTEDDVYLSFLPLAHILDRTVEEYFFRNGASVGYYHGD 300

Query: 301 LNAIKDDLMELKPTLLVGVPRVFERIHEGIKQAVQILNPVRRKAFDLLYKYKLSWLNSGY 360
           L+AIKDDLMELKPTLLVGVPRVFE+IHEGIK+AVQ+LNPVRRKAFDLLYKYKLSW+NSGY
Sbjct: 301 LDAIKDDLMELKPTLLVGVPRVFEKIHEGIKEAVQVLNPVRRKAFDLLYKYKLSWMNSGY 360

Query: 361 KQKYASPLADFLAFRKVKARLGGRLRLIISGGAALSSEVEEFLRVTCCAFFVQGYGLTET 420
           KQK ASPLADFLAFRKVKARLGGRLRLIISGGAALS+EVEEFLRVTCCA FVQGYGLTET
Sbjct: 361 KQKDASPLADFLAFRKVKARLGGRLRLIISGGAALSAEVEEFLRVTCCALFVQGYGLTET 420

Query: 421 CGPTTIGFPDEMCMLGTVGAVTLYNELRLEEVPEMGYNPLGERPCGEICVRGKTVSNEYY 480
           CGPTT+GFPDEMCMLG VGAVTLYNELRLEEVPEMGY+PLG+RPCGEICVRGKTV NEYY
Sbjct: 421 CGPTTVGFPDEMCMLGNVGAVTLYNELRLEEVPEMGYDPLGDRPCGEICVRGKTVFNEYY 480

Query: 481 KNPELTKEAIKDGWFHTGDIGEILPNGVLKIIDRKKNLIKLSQGEYIALEYLENVYSITP 540
           KNPELTKE+IKDGWFHTGDIGEI PNGV+KIIDRKKNLIKLSQGEYIALEYLENVYS+TP
Sbjct: 481 KNPELTKESIKDGWFHTGDIGEIFPNGVVKIIDRKKNLIKLSQGEYIALEYLENVYSVTP 540

Query: 541 IVEDIWIFGNSFKSMLVAVVVLHEENTKKWANSNGFLCSFSELCSLEQLRHYVLSELTST 600
           IVEDIWI+GNSFKS LVAVVVLHEENTKKWANSNGFLCSFSELC LEQLR YVLSELTST
Sbjct: 541 IVEDIWIYGNSFKSKLVAVVVLHEENTKKWANSNGFLCSFSELCCLEQLRQYVLSELTST 600

Query: 601 AERNKLKRFEYIKGVVLESRPFDIEDDLVTATLKKKRNKLLKHYEEEINAEYKNLEANR 660
           AER KLKRFEYIKGVVLESRPFDIED+LVTATLKKKRN L+KHYE+EINAEYKNLEA R
Sbjct: 601 AERTKLKRFEYIKGVVLESRPFDIEDELVTATLKKKRNNLVKHYEKEINAEYKNLEAKR 659

BLAST of Lag0031066 vs. NCBI nr
Match: XP_022948543.1 (long chain acyl-CoA synthetase 1-like [Cucurbita moschata])

HSP 1 Score: 1253.0 bits (3241), Expect = 0.0e+00
Identity = 599/659 (90.90%), Postives = 637/659 (96.66%), Query Frame = 0

Query: 1   MKSFSIKVEEGREGKDGKPSVGPVYRNVLSKDGFPPLYEDLSTSWDLFSVSAKKYAEKQM 60
           MKSFSIKVEEG EGKDGKPSVGPVYRNVL+KDGFPPLYEDLST+WD+FSVSAKKYA K+M
Sbjct: 1   MKSFSIKVEEGMEGKDGKPSVGPVYRNVLTKDGFPPLYEDLSTTWDIFSVSAKKYAGKRM 60

Query: 61  LGWRKFVEGKPGPYIWKTYKEVYVDVLNIGSALRAAGAEPGSRIGIYGANCPQWIIAMEA 120
           LGWRKFVEGKPGPYIWKTY+EVYVDVLNIGSALRA GA+ GSRIGIYGANCPQWIIAMEA
Sbjct: 61  LGWRKFVEGKPGPYIWKTYQEVYVDVLNIGSALRAVGAKAGSRIGIYGANCPQWIIAMEA 120

Query: 121 CNAHSLICVPLYDTLGHGAVNFIIDHAEIDIVFVQDKKAKELLNSESTNPKRLKVVVCFT 180
           CNAHSL+ VPLYDTLGHGAVN+II+HAEIDI+F+QDKKAKELL+S STNPKRLKV++CFT
Sbjct: 121 CNAHSLVAVPLYDTLGHGAVNYIIEHAEIDIIFIQDKKAKELLSSGSTNPKRLKVIICFT 180

Query: 181 SLKGEEKTSAVGNGMTPYSWDEFLRLGKENPSEITPPKPLDICTIMYTSGTSGTPKGVVL 240
           SLKGEEKTSAVGNG+ PYSWDEFLRLGKE+P EI PP+P D+CTIMYTSGTSGTPKGVVL
Sbjct: 181 SLKGEEKTSAVGNGIKPYSWDEFLRLGKEDPFEIVPPRPFDVCTIMYTSGTSGTPKGVVL 240

Query: 241 THETVTTFIRGVDIYLEQFEDKMTEDDVYLSFLPLAHILDRTVEEYFFRKGASVGYYHGD 300
           THET+TTFIRGVD+++EQFEDKMTEDDVYLSFLPLAHILDRTVEEYFFR GASVGYYHGD
Sbjct: 241 THETITTFIRGVDVFMEQFEDKMTEDDVYLSFLPLAHILDRTVEEYFFRNGASVGYYHGD 300

Query: 301 LNAIKDDLMELKPTLLVGVPRVFERIHEGIKQAVQILNPVRRKAFDLLYKYKLSWLNSGY 360
           L+AIKDDLMELKPTLLVGVPRVFE+IHEGIK+AVQ+LNPVRRKAFDLLYKYKLSW+NSGY
Sbjct: 301 LDAIKDDLMELKPTLLVGVPRVFEKIHEGIKEAVQVLNPVRRKAFDLLYKYKLSWMNSGY 360

Query: 361 KQKYASPLADFLAFRKVKARLGGRLRLIISGGAALSSEVEEFLRVTCCAFFVQGYGLTET 420
           KQK ASPLADFLAFRKVKARLGGRLRLIISGGAALS+EVEEFLRVTCCA FVQGYGLTET
Sbjct: 361 KQKDASPLADFLAFRKVKARLGGRLRLIISGGAALSAEVEEFLRVTCCALFVQGYGLTET 420

Query: 421 CGPTTIGFPDEMCMLGTVGAVTLYNELRLEEVPEMGYNPLGERPCGEICVRGKTVSNEYY 480
           CGPTT+GFPDEMCMLG VGAVTLYNELRLEEVPEMGY+PLG+RPCGEICVRGKTV NEYY
Sbjct: 421 CGPTTVGFPDEMCMLGNVGAVTLYNELRLEEVPEMGYDPLGDRPCGEICVRGKTVFNEYY 480

Query: 481 KNPELTKEAIKDGWFHTGDIGEILPNGVLKIIDRKKNLIKLSQGEYIALEYLENVYSITP 540
           KNPELTKE+IKDGWFHTGDIGEI PNGV+KIIDRKKNLIKLSQGEYIALEYLENVYS+TP
Sbjct: 481 KNPELTKESIKDGWFHTGDIGEIFPNGVVKIIDRKKNLIKLSQGEYIALEYLENVYSVTP 540

Query: 541 IVEDIWIFGNSFKSMLVAVVVLHEENTKKWANSNGFLCSFSELCSLEQLRHYVLSELTST 600
           IVEDIWI+GNSFKS LVAVVVLHEENTKKWANSNGFLCSFSELC LEQLR YVLSELTST
Sbjct: 541 IVEDIWIYGNSFKSKLVAVVVLHEENTKKWANSNGFLCSFSELCCLEQLRQYVLSELTST 600

Query: 601 AERNKLKRFEYIKGVVLESRPFDIEDDLVTATLKKKRNKLLKHYEEEINAEYKNLEANR 660
           AER KLKRFEYIKGVVLESRPFDIED+LVTATLKKKRN L+KHYE+EINAEYKNLEA R
Sbjct: 601 AERTKLKRFEYIKGVVLESRPFDIEDELVTATLKKKRNNLVKHYEKEINAEYKNLEAKR 659

BLAST of Lag0031066 vs. ExPASy Swiss-Prot
Match: O22898 (Long chain acyl-CoA synthetase 1 OS=Arabidopsis thaliana OX=3702 GN=LACS1 PE=2 SV=1)

HSP 1 Score: 946.8 bits (2446), Expect = 1.3e-274
Identity = 440/659 (66.77%), Postives = 539/659 (81.79%), Query Frame = 0

Query: 1   MKSFSIKVEEGREGKDGKPSVGPVYRNVLSKDGFPPLYEDLSTSWDLFSVSAKKYAEKQM 60
           MKSF+ KVEEG +G DGKPSVGPVYRN+LS+ GFPP+  +++T+WD+FS S +K+ +  M
Sbjct: 1   MKSFAAKVEEGVKGIDGKPSVGPVYRNLLSEKGFPPIDSEITTAWDIFSKSVEKFPDNNM 60

Query: 61  LGWRKFVEGKPGPYIWKTYKEVYVDVLNIGSALRAAGAEPGSRIGIYGANCPQWIIAMEA 120
           LGWR+ V+ K GPY+WKTYKEVY +VL IGSALRAAGAEPGSR+GIYG NCPQWIIAMEA
Sbjct: 61  LGWRRIVDEKVGPYMWKTYKEVYEEVLQIGSALRAAGAEPGSRVGIYGVNCPQWIIAMEA 120

Query: 121 CNAHSLICVPLYDTLGHGAVNFIIDHAEIDIVFVQDKKAKELLNSESTNPKRLKVVVCFT 180
           C AH+LICVPLYDTLG GAV++I++HAEID VFVQD K K LL  +    KRLK +V FT
Sbjct: 121 CAAHTLICVPLYDTLGSGAVDYIVEHAEIDFVFVQDTKIKGLLEPDCKCAKRLKAIVSFT 180

Query: 181 SLKGEEKTSAVGNGMTPYSWDEFLRLGKENPSEITPPKPLDICTIMYTSGTSGTPKGVVL 240
           ++  E    A   G+  YSW +FL +G+E P +  PPK  +ICTIMYTSGTSG PKGVVL
Sbjct: 181 NVSDELSHKASEIGVKTYSWIDFLHMGREKPEDTNPPKAFNICTIMYTSGTSGDPKGVVL 240

Query: 241 THETVTTFIRGVDIYLEQFEDKMTEDDVYLSFLPLAHILDRTVEEYFFRKGASVGYYHGD 300
           TH+ V TF+ G+D+Y++QFEDKMT DDVYLSFLPLAHILDR  EEYFFRKGASVGYYHG+
Sbjct: 241 THQAVATFVVGMDLYMDQFEDKMTHDDVYLSFLPLAHILDRMNEEYFFRKGASVGYYHGN 300

Query: 301 LNAIKDDLMELKPTLLVGVPRVFERIHEGIKQAVQILNPVRRKAFDLLYKYKLSWLNSGY 360
           LN ++DD+ ELKPT L GVPRVFERIHEGI++A+Q LNP RR  F+ LYK+KL+WLN GY
Sbjct: 301 LNVLRDDIQELKPTYLAGVPRVFERIHEGIQKALQELNPRRRFIFNALYKHKLAWLNRGY 360

Query: 361 KQKYASPLADFLAFRKVKARLGGRLRLIISGGAALSSEVEEFLRVTCCAFFVQGYGLTET 420
               ASP+ADF+AFRK++ +LGGR+RL++SGGA LS E+EEFLRVTCC F VQGYGLTET
Sbjct: 361 SHSKASPMADFIAFRKIRDKLGGRIRLLVSGGAPLSPEIEEFLRVTCCCFVVQGYGLTET 420

Query: 421 CGPTTIGFPDEMCMLGTVGAVTLYNELRLEEVPEMGYNPLGERPCGEICVRGKTVSNEYY 480
            G T +GFPDEMCMLGTVG   +YNE+RLEEV EMGY+PLGE P GEIC+RG+ + + YY
Sbjct: 421 LGGTALGFPDEMCMLGTVGIPAVYNEIRLEEVSEMGYDPLGENPAGEICIRGQCMFSGYY 480

Query: 481 KNPELTKEAIKDGWFHTGDIGEILPNGVLKIIDRKKNLIKLSQGEYIALEYLENVYSITP 540
           KNPELT+E +KDGWFHTGDIGEILPNGVLKIIDRKKNLIKLSQGEY+ALE+LEN++    
Sbjct: 481 KNPELTEEVMKDGWFHTGDIGEILPNGVLKIIDRKKNLIKLSQGEYVALEHLENIFGQNS 540

Query: 541 IVEDIWIFGNSFKSMLVAVVVLHEENTKKWANSNGFLCSFSELCSLEQLRHYVLSELTST 600
           +V+DIW++G+SFKSMLVAVVV + E   +WA   GF   F ELCS  +L+ +++SEL ST
Sbjct: 541 VVQDIWVYGDSFKSMLVAVVVPNPETVNRWAKDLGFTKPFEELCSFPELKEHIISELKST 600

Query: 601 AERNKLKRFEYIKGVVLESRPFDIEDDLVTATLKKKRNKLLKHYEEEINAEYKNLEANR 660
           AE+NKL++FEYIK V +E++PFD+E DLVTATLK +RN LLK+Y+ +I+  Y+ L + +
Sbjct: 601 AEKNKLRKFEYIKAVTVETKPFDVERDLVTATLKNRRNNLLKYYQVQIDEMYRKLASKK 659

BLAST of Lag0031066 vs. ExPASy Swiss-Prot
Match: Q9T0A0 (Long chain acyl-CoA synthetase 4 OS=Arabidopsis thaliana OX=3702 GN=LACS4 PE=2 SV=1)

HSP 1 Score: 677.2 bits (1746), Expect = 1.9e-193
Identity = 318/656 (48.48%), Postives = 446/656 (67.99%), Query Frame = 0

Query: 2   KSFSIKVEEGREGKDGKPSVGPVYRNVLSKDGFPPLYEDLSTSWDLFSVSAKKYAEKQML 61
           K +  +VEEG+EG DG+PSVGPVYR++ +KDGFP   E + + WD+F +S +KY    ML
Sbjct: 5   KKYIFQVEEGKEGSDGRPSVGPVYRSIFAKDGFPDPIEGMDSCWDVFRMSVEKYPNNPML 64

Query: 62  GWRKFVEGKPGPYIWKTYKEVYVDVLNIGSALRAAGAEPGSRIGIYGANCPQWIIAMEAC 121
           G R+ V+GKPG Y+W+TY+EVY  V+ +G++LR+ G +  ++ GIYGAN P+WII+MEAC
Sbjct: 65  GRREIVDGKPGKYVWQTYQEVYDIVMKLGNSLRSVGVKDEAKCGIYGANSPEWIISMEAC 124

Query: 122 NAHSLICVPLYDTLGHGAVNFIIDHAEIDIVFVQDKKAKELLNSESTNPKRLKVVVCFTS 181
           NAH L CVPLYDTLG  AV FII H+E+ IVFV++KK  EL  +   + + +K VV F  
Sbjct: 125 NAHGLYCVPLYDTLGADAVEFIISHSEVSIVFVEEKKISELFKTCPNSTEYMKTVVSFGG 184

Query: 182 LKGEEKTSAVGNGMTPYSWDEFLRLGKENPSEITPPKPLDICTIMYTSGTSGTPKGVVLT 241
           +  E+K  A   G+  Y+WDEFL+LG+    ++   K  DICTIMYTSGT+G PKGV+++
Sbjct: 185 VSREQKEEAETFGLVIYAWDEFLKLGEGKQYDLPIKKKSDICTIMYTSGTTGDPKGVMIS 244

Query: 242 HETVTTFIRGVDIYLEQFEDKMTEDDVYLSFLPLAHILDRTVEEYFFRKGASVGYYHGDL 301
           +E++ T I GV   L+   + +T  DVYLS+LPLAHI DR +EE F + GA++G++ GD+
Sbjct: 245 NESIVTLIAGVIRLLKSANEALTVKDVYLSYLPLAHIFDRVIEECFIQHGAAIGFWRGDV 304

Query: 302 NAIKDDLMELKPTLLVGVPRVFERIHEGIKQAVQILNPVRRKAFDLLYKYKLSWLNSGYK 361
             + +DL ELKPT+   VPRV +R++ G+++ +     +++  FD  + YK  ++  G  
Sbjct: 305 KLLIEDLAELKPTIFCAVPRVLDRVYSGLQKKLSDGGFLKKFIFDSAFSYKFGYMKKGQS 364

Query: 362 QKYASPLADFLAFRKVKARLGGRLRLIISGGAALSSEVEEFLRVTCCAFFVQGYGLTETC 421
              ASPL D L F KVK  LGG +R+I+SG A L+S VE FLRV  C   +QGYGLTE+C
Sbjct: 365 HVEASPLFDKLVFSKVKQGLGGNVRIILSGAAPLASHVESFLRVVACCHVLQGYGLTESC 424

Query: 422 GPTTIGFPDEMCMLGTVGAVTLYNELRLEEVPEMGYNPLGERPCGEICVRGKTVSNEYYK 481
             T +  PDE+ MLGTVG      ++RLE VPEM Y+ L     GEIC+RGKT+ + YYK
Sbjct: 425 AGTFVSLPDELGMLGTVGPPVPNVDIRLESVPEMEYDALASTARGEICIRGKTLFSGYYK 484

Query: 482 NPELTKEAIKDGWFHTGDIGEILPNGVLKIIDRKKNLIKLSQGEYIALEYLENVYSITPI 541
             +LTKE + DGW HTGD+GE  P+G +KIIDRKKN+ KLSQGEY+A+E +EN+Y     
Sbjct: 485 REDLTKEVLIDGWLHTGDVGEWQPDGSMKIIDRKKNIFKLSQGEYVAVENIENIYGEVQA 544

Query: 542 VEDIWIFGNSFKSMLVAVVVLHEENTKKWANSNGFLCSFSELCSLEQLRHYVLSELTSTA 601
           V+ +W++GNSF+S L+A+   ++   ++WA  NG    +  LC  E+ + ++L EL   A
Sbjct: 545 VDSVWVYGNSFESFLIAIANPNQHILERWAAENGVSGDYDALCQNEKAKEFILGELVKMA 604

Query: 602 ERNKLKRFEYIKGVVLESRPFDIEDDLVTATLKKKRNKLLKHYEEEINAEYKNLEA 658
           +  K+K FE IK + L+  PFD+E DL+T T KKKR +LLK+Y+  I+  YK + A
Sbjct: 605 KEKKMKGFEIIKAIHLDPVPFDMERDLLTPTFKKKRPQLLKYYQSVIDEMYKTINA 660

BLAST of Lag0031066 vs. ExPASy Swiss-Prot
Match: Q9T009 (Long chain acyl-CoA synthetase 5 OS=Arabidopsis thaliana OX=3702 GN=LACS5 PE=2 SV=1)

HSP 1 Score: 673.7 bits (1737), Expect = 2.1e-192
Identity = 322/652 (49.39%), Postives = 437/652 (67.02%), Query Frame = 0

Query: 2   KSFSIKVEEGREGKDGKPSVGPVYRNVLSKDGFPPLYEDLSTSWDLFSVSAKKYAEKQML 61
           K F  +VE  +E  DG PSVGPVYR+  +++GFP   + + + WD+F  + +KY   +ML
Sbjct: 5   KRFIFEVEAAKEATDGNPSVGPVYRSTFAQNGFPNPIDGIQSCWDIFRTAVEKYPNNRML 64

Query: 62  GWRKFVEGKPGPYIWKTYKEVYVDVLNIGSALRAAGAEPGSRIGIYGANCPQWIIAMEAC 121
           G R+   GK G Y+WKTYKEVY  V+ +G++LR+ G + G + GIYG NC +WII+MEAC
Sbjct: 65  GRREISNGKAGKYVWKTYKEVYDIVIKLGNSLRSCGIKEGEKCGIYGINCCEWIISMEAC 124

Query: 122 NAHSLICVPLYDTLGHGAVNFIIDHAEIDIVFVQDKKAKELLNSESTNPKRLKVVVCFTS 181
           NAH L CVPLYDTLG GAV FII HAE+ I FV++KK  EL  +   + K +K VV F  
Sbjct: 125 NAHGLYCVPLYDTLGAGAVEFIISHAEVSIAFVEEKKIPELFKTCPNSTKYMKTVVSFGG 184

Query: 182 LKGEEKTSAVGNGMTPYSWDEFLRLGKENPSEITPPKPLDICTIMYTSGTSGTPKGVVLT 241
           +K E+K  A   G+  +SWDEFL+LG+    E+   KP DICTIMYTSGT+G PKGV+++
Sbjct: 185 VKPEQKEEAEKLGLVIHSWDEFLKLGEGKQYELPIKKPSDICTIMYTSGTTGDPKGVMIS 244

Query: 242 HETVTTFIRGVDIYLEQFEDKMTEDDVYLSFLPLAHILDRTVEEYFFRKGASVGYYHGDL 301
           +E++ T   GV  +L      ++E DVY+S+LPLAH+ DR +EE   + G S+G++ GD+
Sbjct: 245 NESIVTITTGVMHFLGNVNASLSEKDVYISYLPLAHVFDRAIEECIIQVGGSIGFWRGDV 304

Query: 302 NAIKDDLMELKPTLLVGVPRVFERIHEGIKQAVQILNPVRRKAFDLLYKYKLSWLNSGYK 361
             + +DL ELKP++   VPRV +R++ G++Q +      ++K FD+ + YK   +  G  
Sbjct: 305 KLLIEDLGELKPSIFCAVPRVLDRVYTGLQQKLSGGGFFKKKVFDVAFSYKFGNMKKGQS 364

Query: 362 QKYASPLADFLAFRKVKARLGGRLRLIISGGAALSSEVEEFLRVTCCAFFVQGYGLTETC 421
              ASP  D L F KVK  LGG +R+I+SG A L+S +E FLRV  C   +QGYGLTE+C
Sbjct: 365 HVAASPFCDKLVFNKVKQGLGGNVRIILSGAAPLASHIESFLRVVACCNVLQGYGLTESC 424

Query: 422 GPTTIGFPDEMCMLGTVGAVTLYNELRLEEVPEMGYNPLGERPCGEICVRGKTVSNEYYK 481
             T   FPDE+ MLGTVG      ++RLE VPEM Y+ LG  P GEIC+RGKT+ + YYK
Sbjct: 425 AGTFATFPDELDMLGTVGPPVPNVDIRLESVPEMNYDALGSTPRGEICIRGKTLFSGYYK 484

Query: 482 NPELTKEAIKDGWFHTGDIGEILPNGVLKIIDRKKNLIKLSQGEYIALEYLENVYSITPI 541
             +LTKE   DGW HTGD+GE  PNG +KIIDRKKN+ KL+QGEY+A+E LENVYS   +
Sbjct: 485 REDLTKEVFIDGWLHTGDVGEWQPNGSMKIIDRKKNIFKLAQGEYVAVENLENVYSQVEV 544

Query: 542 VEDIWIFGNSFKSMLVAVVVLHEENTKKWANSNGFLCSFSELCSLEQLRHYVLSELTSTA 601
           +E IW++GNSF+S LVA+    ++  ++WA  NG    F+ +C   + + ++L EL  TA
Sbjct: 545 IESIWVYGNSFESFLVAIANPAQQTLERWAVENGVNGDFNSICQNAKAKAFILGELVKTA 604

Query: 602 ERNKLKRFEYIKGVVLESRPFDIEDDLVTATLKKKRNKLLKHYEEEINAEYK 654
           + NKLK FE IK V LE   FD+E DL+T T KKKR +LLK+Y+  I+  YK
Sbjct: 605 KENKLKGFEIIKDVHLEPVAFDMERDLLTPTYKKKRPQLLKYYQNVIHEMYK 656

BLAST of Lag0031066 vs. ExPASy Swiss-Prot
Match: Q9C7W4 (Long chain acyl-CoA synthetase 3 OS=Arabidopsis thaliana OX=3702 GN=LACS3 PE=2 SV=1)

HSP 1 Score: 667.2 bits (1720), Expect = 2.0e-190
Identity = 315/651 (48.39%), Postives = 446/651 (68.51%), Query Frame = 0

Query: 4   FSIKVEEGREGKD-GKPSVGPVYRNVLSKDGFPPLYEDLSTSWDLFSVSAKKYAEKQMLG 63
           + ++VE+G++G D G PSVGPVYR++ +KDGFP   +DL ++WD+F +S +K     MLG
Sbjct: 6   YIVEVEKGKQGVDGGSPSVGPVYRSIYAKDGFPEPPDDLVSAWDIFRLSVEKSPNNPMLG 65

Query: 64  WRKFVEGKPGPYIWKTYKEVYVDVLNIGSALRAAGAEPGSRIGIYGANCPQWIIAMEACN 123
            R+ V+GK G Y+W+TYKEV+  V+ +G+++R  G   G + GIYGAN P+WII+MEACN
Sbjct: 66  RREIVDGKAGKYVWQTYKEVHNVVIKLGNSIRTIGVGKGDKCGIYGANSPEWIISMEACN 125

Query: 124 AHSLICVPLYDTLGHGAVNFIIDHAEIDIVFVQDKKAKELLNSESTNPKRLKVVVCFTSL 183
           AH L CVPLYDTLG GA+ FII HAE+ + F ++ K  ELL +   + K LK +V F  +
Sbjct: 126 AHGLYCVPLYDTLGAGAIEFIICHAEVSLAFAEENKISELLKTAPKSTKYLKYIVSFGEV 185

Query: 184 KGEEKTSAVGNGMTPYSWDEFLRLGKENPSEITPPKPLDICTIMYTSGTSGTPKGVVLTH 243
              ++  A  + +T YSWD+FL+LG+    E+   +  D+CTIMYTSGT+G PKGV+LT+
Sbjct: 186 TNNQRVEAERHRLTIYSWDQFLKLGEGKHYELPEKRRSDVCTIMYTSGTTGDPKGVLLTN 245

Query: 244 ETVTTFIRGVDIYLEQFEDKMTEDDVYLSFLPLAHILDRTVEEYFFRKGASVGYYHGDLN 303
           E++   + GV   L+  ++++T  DVYLS+LPLAHI DR +EE    + AS+G++ GD+ 
Sbjct: 246 ESIIHLLEGVKKLLKTIDEELTSKDVYLSYLPLAHIFDRVIEELCIYEAASIGFWRGDVK 305

Query: 304 AIKDDLMELKPTLLVGVPRVFERIHEGIKQAVQILNPVRRKAFDLLYKYKLSWLNSGYKQ 363
            + +D+  LKPT+   VPRV ERI+ G++Q +     V++K F+  +KYK   +  G   
Sbjct: 306 ILIEDIAALKPTVFCAVPRVLERIYTGLQQKLSDGGFVKKKLFNFAFKYKHKNMEKGQPH 365

Query: 364 KYASPLADFLAFRKVKARLGGRLRLIISGGAALSSEVEEFLRVTCCAFFVQGYGLTETCG 423
           + ASP+AD + F+KVK  LGG +RLI+SG A L++ +E FLRV  CA  +QGYGLTE+CG
Sbjct: 366 EQASPIADKIVFKKVKEGLGGNVRLILSGAAPLAAHIESFLRVVACAHVLQGYGLTESCG 425

Query: 424 PTTIGFPDEMCMLGTVGAVTLYNELRLEEVPEMGYNPLGERPCGEICVRGKTVSNEYYKN 483
            T +  P+E+ MLGTVG      ++RLE VPEMGY+ L   P GEIC+RGKT+ + YYK 
Sbjct: 426 GTFVSIPNELSMLGTVGPPVPNVDIRLESVPEMGYDALASNPRGEICIRGKTLFSGYYKR 485

Query: 484 PELTKEAIKDGWFHTGDIGEILPNGVLKIIDRKKNLIKLSQGEYIALEYLENVYSITPIV 543
            +LT+E   DGW HTGD+GE  P+G +KIIDRKKN+ KLSQGEY+A+E LEN+YS    +
Sbjct: 486 EDLTQEVFIDGWLHTGDVGEWQPDGAMKIIDRKKNIFKLSQGEYVAVENLENIYSHVAAI 545

Query: 544 EDIWIFGNSFKSMLVAVVVLHEENTKKWANSNGFLCSFSELCSLEQLRHYVLSELTSTAE 603
           E IW++GNS++S LVAVV   +   + WA  +     F  +C  ++ + +VL E    A+
Sbjct: 546 ESIWVYGNSYESYLVAVVCPSKIQIEHWAKEHKVSGDFESICRNQKTKEFVLGEFNRVAK 605

Query: 604 RNKLKRFEYIKGVVLESRPFDIEDDLVTATLKKKRNKLLKHYEEEINAEYK 654
             KLK FE IKGV L++ PFD+E DL+T + K KR +LLK+Y++EI+  YK
Sbjct: 606 DKKLKGFELIKGVHLDTVPFDMERDLITPSYKMKRPQLLKYYQKEIDEMYK 656

BLAST of Lag0031066 vs. ExPASy Swiss-Prot
Match: M4ISH1 (Probable CoA ligase CCL6 OS=Humulus lupulus OX=3486 GN=CCL6 PE=2 SV=1)

HSP 1 Score: 621.3 bits (1601), Expect = 1.2e-176
Identity = 300/657 (45.66%), Postives = 419/657 (63.77%), Query Frame = 0

Query: 3   SFSIKVEEGREGKDGKPSVGPVYRNVLSKDGFPPLYEDLSTSWDLFSVSAKKYAEKQMLG 62
           S+++KVEE R   +  PS GPVYR++ ++DG   L E L + W+  S S K+  +  MLG
Sbjct: 2   SYTVKVEEARPATEKMPSAGPVYRSIYARDGLLELPEGLQSPWEFLSGSVKRSPKTPMLG 61

Query: 63  WRKFVEGKPGPYIWKTYKEVYVDVLNIGSALRAAGAEPGSRIGIYGANCPQWIIAMEACN 122
            R+  + + GPY+W TY+EV+ + + + SA+R+ G  PG R GIYG NCPQWI+AM+AC 
Sbjct: 62  RRQIKDSEAGPYVWLTYQEVHDEAIRMASAMRSRGVNPGDRCGIYGTNCPQWIVAMQACY 121

Query: 123 AHSLICVPLYDTLGHGAVNFIIDHAEIDIVFVQDKKAKELLNSESTNPKRLKVVVCFTSL 182
           +H++  VPLYDTLG  AV FII+H E+ I FVQ+ K   +L+        LK +V F ++
Sbjct: 122 SHAITYVPLYDTLGPNAVEFIINHGEVSIAFVQENKISAILSCLPNCSSLLKTIVSFGNI 181

Query: 183 KGEEKTSAVGNGMTPYSWDEFLRLGKENPSEITPPKPLDICTIMYTSGTSGTPKGVVLTH 242
              +K      G++ +SW+EF +LG  +  E+      DICT+MYTSG +G PKGV+LT+
Sbjct: 182 TSVQKKETEALGVSCFSWEEFSQLGNLS-GELPEKHRTDICTLMYTSGATGEPKGVILTN 241

Query: 243 ETVTTFIRGVDIYLEQFEDKMTEDDVYLSFLPLAHILDRTVEEYFFRKGASVGYYHGDLN 302
           E +   I   D  LE  +   +E+DVY S+LPLAH+ D+ VE Y   KG+++GY+ GD+ 
Sbjct: 242 EAIMAEILSTDNMLELTDKVFSEEDVYFSYLPLAHVYDQIVENYCIYKGSAIGYWRGDIR 301

Query: 303 AIKDDLMELKPTLLVGVPRVFERIHEGIKQAVQILNPVRRKAFDLLYKYKLSWLNSGYKQ 362
            + DDL ELKPT+  GVPRV++RI+ GI   V     +++  F   Y YK++ +  G   
Sbjct: 302 FLMDDLQELKPTIFCGVPRVYDRIYAGIFHKVSSGGTLKKMLFQYAYNYKMANMEKGLPH 361

Query: 363 KYASPLADFLAFRKVKARLGGRLRLIISGGAALSSEVEEFLRVTCCAFFVQGYGLTETCG 422
             A+PL D L F K+K   GGR+RL+ SG A L   VEE+LRVT CA   QGYGLTE+CG
Sbjct: 362 GQAAPLMDKLFFDKIKQGFGGRIRLMFSGAAPLPHHVEEYLRVTSCAALSQGYGLTESCG 421

Query: 423 PTTIGFPDEMCMLGTVGAVTLYNELRLEEVPEMGYNPLGERPCGEICVRGKTVSNEYYKN 482
                  +   M+GTVG      E RLE VPEMGY+ L ++P GEIC+RG T+ + Y+K 
Sbjct: 422 GCLTSIANIFPMIGTVGVPMTTIEARLESVPEMGYDALSDKPRGEICLRGTTLFSGYHKR 481

Query: 483 PELTKEAIKDGWFHTGDIGEILPNGVLKIIDRKKNLIKLSQGEYIALEYLENVYSITPIV 542
            +LTK+ + DGWFHTGDIGE  PNG +KIIDRKKN+ KLSQGEY+ +E +E +Y   P++
Sbjct: 482 EDLTKDVLVDGWFHTGDIGEWQPNGAMKIIDRKKNIFKLSQGEYVPVENIEGIYLQCPLI 541

Query: 543 EDIWIFGNSFKSMLVAVVVLHEENTKKWANSNGFLCSFSELCSLEQLRHYVLSELTSTAE 602
             IW++GNSF+S LVAVVV      + WA +      F  LC   +   Y+L EL STA+
Sbjct: 542 ASIWVYGNSFESFLVAVVVPERLALENWAANRNLTDDFKSLCENPKASKYILDELNSTAK 601

Query: 603 RNKLKRFEYIKGVVLESRPFDIEDDLVTATLKKKRNKLLKHYEEEINAEYKNLEANR 660
           +++L+ FE +K V LE  PFD+E DL+T T K KR +LLK+Y++ ++  Y   +  R
Sbjct: 602 KHQLRGFEMLKAVYLEPNPFDMERDLITPTFKLKRPQLLKYYKDHVDKLYSEAKEAR 657

BLAST of Lag0031066 vs. ExPASy TrEMBL
Match: A0A6J1K7Y2 (long chain acyl-CoA synthetase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111493051 PE=4 SV=1)

HSP 1 Score: 1254.6 bits (3245), Expect = 0.0e+00
Identity = 600/659 (91.05%), Postives = 637/659 (96.66%), Query Frame = 0

Query: 1   MKSFSIKVEEGREGKDGKPSVGPVYRNVLSKDGFPPLYEDLSTSWDLFSVSAKKYAEKQM 60
           MKSFSIKVEEG EGKDGKPSVGPVYRNVL+KDGFPPLYEDLST+WD+FSVSAKKYA K+M
Sbjct: 1   MKSFSIKVEEGTEGKDGKPSVGPVYRNVLTKDGFPPLYEDLSTTWDIFSVSAKKYAGKRM 60

Query: 61  LGWRKFVEGKPGPYIWKTYKEVYVDVLNIGSALRAAGAEPGSRIGIYGANCPQWIIAMEA 120
           LGWRKFVEGKPGPYIWKTY+EVYVDVLNIGSALRA GA+ GSRIGIYGANCPQWIIAMEA
Sbjct: 61  LGWRKFVEGKPGPYIWKTYQEVYVDVLNIGSALRAVGAKAGSRIGIYGANCPQWIIAMEA 120

Query: 121 CNAHSLICVPLYDTLGHGAVNFIIDHAEIDIVFVQDKKAKELLNSESTNPKRLKVVVCFT 180
           CNAHSL+ VPLYDTLGHGAVN+II+HAEIDI+F+QDKKAKELL+SESTNPKRLKV++CFT
Sbjct: 121 CNAHSLVAVPLYDTLGHGAVNYIIEHAEIDIIFIQDKKAKELLSSESTNPKRLKVIICFT 180

Query: 181 SLKGEEKTSAVGNGMTPYSWDEFLRLGKENPSEITPPKPLDICTIMYTSGTSGTPKGVVL 240
           SLKGEEK +AVGNG+ PYSWDEFLRLGKE+P EI PP+PLD+CTIMYTSGTSGTPKGVVL
Sbjct: 181 SLKGEEKAAAVGNGIKPYSWDEFLRLGKEDPFEIVPPRPLDVCTIMYTSGTSGTPKGVVL 240

Query: 241 THETVTTFIRGVDIYLEQFEDKMTEDDVYLSFLPLAHILDRTVEEYFFRKGASVGYYHGD 300
           THET+TTFIRGVD+++EQFEDKMTEDDVYLSFLPLAHILDRTVEEYFFR GASVGYYHGD
Sbjct: 241 THETITTFIRGVDVFMEQFEDKMTEDDVYLSFLPLAHILDRTVEEYFFRNGASVGYYHGD 300

Query: 301 LNAIKDDLMELKPTLLVGVPRVFERIHEGIKQAVQILNPVRRKAFDLLYKYKLSWLNSGY 360
           L+AIKDDLMELKPTLLVGVPRVFE+IHEGIK+AVQ+LNPVRRKAFDLLYKYKLSW+NSGY
Sbjct: 301 LDAIKDDLMELKPTLLVGVPRVFEKIHEGIKEAVQVLNPVRRKAFDLLYKYKLSWMNSGY 360

Query: 361 KQKYASPLADFLAFRKVKARLGGRLRLIISGGAALSSEVEEFLRVTCCAFFVQGYGLTET 420
           KQK ASPLADFLAFRKVKARLGGRLRLIISGGAALS+EVEEFLRVTCCA FVQGYGLTET
Sbjct: 361 KQKDASPLADFLAFRKVKARLGGRLRLIISGGAALSAEVEEFLRVTCCALFVQGYGLTET 420

Query: 421 CGPTTIGFPDEMCMLGTVGAVTLYNELRLEEVPEMGYNPLGERPCGEICVRGKTVSNEYY 480
           CGPTT+GFPDEMCMLG VGAVTLYNELRLEEVPEMGYNPLG+RPCGEICVRGKTV NEYY
Sbjct: 421 CGPTTVGFPDEMCMLGKVGAVTLYNELRLEEVPEMGYNPLGDRPCGEICVRGKTVFNEYY 480

Query: 481 KNPELTKEAIKDGWFHTGDIGEILPNGVLKIIDRKKNLIKLSQGEYIALEYLENVYSITP 540
           KNPELTKE+IKDGWFHTGDIGEI PNGV+KIIDRKKNLIKLSQGEYIALEYLENVYS+TP
Sbjct: 481 KNPELTKESIKDGWFHTGDIGEIFPNGVVKIIDRKKNLIKLSQGEYIALEYLENVYSVTP 540

Query: 541 IVEDIWIFGNSFKSMLVAVVVLHEENTKKWANSNGFLCSFSELCSLEQLRHYVLSELTST 600
           IVEDIWI+GNSFKS LVAVVVLHEENTKKWANSNGFLCSFSELC LEQLR YVL ELTST
Sbjct: 541 IVEDIWIYGNSFKSKLVAVVVLHEENTKKWANSNGFLCSFSELCCLEQLRQYVLFELTST 600

Query: 601 AERNKLKRFEYIKGVVLESRPFDIEDDLVTATLKKKRNKLLKHYEEEINAEYKNLEANR 660
           AER KLKRFEYIKGVVLESRPFDIED+LVTATLKKKRN LLKHYE+EINAEYKNLEA R
Sbjct: 601 AERTKLKRFEYIKGVVLESRPFDIEDELVTATLKKKRNNLLKHYEKEINAEYKNLEAKR 659

BLAST of Lag0031066 vs. ExPASy TrEMBL
Match: A0A6J1G9K8 (long chain acyl-CoA synthetase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111452190 PE=4 SV=1)

HSP 1 Score: 1253.0 bits (3241), Expect = 0.0e+00
Identity = 599/659 (90.90%), Postives = 637/659 (96.66%), Query Frame = 0

Query: 1   MKSFSIKVEEGREGKDGKPSVGPVYRNVLSKDGFPPLYEDLSTSWDLFSVSAKKYAEKQM 60
           MKSFSIKVEEG EGKDGKPSVGPVYRNVL+KDGFPPLYEDLST+WD+FSVSAKKYA K+M
Sbjct: 1   MKSFSIKVEEGMEGKDGKPSVGPVYRNVLTKDGFPPLYEDLSTTWDIFSVSAKKYAGKRM 60

Query: 61  LGWRKFVEGKPGPYIWKTYKEVYVDVLNIGSALRAAGAEPGSRIGIYGANCPQWIIAMEA 120
           LGWRKFVEGKPGPYIWKTY+EVYVDVLNIGSALRA GA+ GSRIGIYGANCPQWIIAMEA
Sbjct: 61  LGWRKFVEGKPGPYIWKTYQEVYVDVLNIGSALRAVGAKAGSRIGIYGANCPQWIIAMEA 120

Query: 121 CNAHSLICVPLYDTLGHGAVNFIIDHAEIDIVFVQDKKAKELLNSESTNPKRLKVVVCFT 180
           CNAHSL+ VPLYDTLGHGAVN+II+HAEIDI+F+QDKKAKELL+S STNPKRLKV++CFT
Sbjct: 121 CNAHSLVAVPLYDTLGHGAVNYIIEHAEIDIIFIQDKKAKELLSSGSTNPKRLKVIICFT 180

Query: 181 SLKGEEKTSAVGNGMTPYSWDEFLRLGKENPSEITPPKPLDICTIMYTSGTSGTPKGVVL 240
           SLKGEEKTSAVGNG+ PYSWDEFLRLGKE+P EI PP+P D+CTIMYTSGTSGTPKGVVL
Sbjct: 181 SLKGEEKTSAVGNGIKPYSWDEFLRLGKEDPFEIVPPRPFDVCTIMYTSGTSGTPKGVVL 240

Query: 241 THETVTTFIRGVDIYLEQFEDKMTEDDVYLSFLPLAHILDRTVEEYFFRKGASVGYYHGD 300
           THET+TTFIRGVD+++EQFEDKMTEDDVYLSFLPLAHILDRTVEEYFFR GASVGYYHGD
Sbjct: 241 THETITTFIRGVDVFMEQFEDKMTEDDVYLSFLPLAHILDRTVEEYFFRNGASVGYYHGD 300

Query: 301 LNAIKDDLMELKPTLLVGVPRVFERIHEGIKQAVQILNPVRRKAFDLLYKYKLSWLNSGY 360
           L+AIKDDLMELKPTLLVGVPRVFE+IHEGIK+AVQ+LNPVRRKAFDLLYKYKLSW+NSGY
Sbjct: 301 LDAIKDDLMELKPTLLVGVPRVFEKIHEGIKEAVQVLNPVRRKAFDLLYKYKLSWMNSGY 360

Query: 361 KQKYASPLADFLAFRKVKARLGGRLRLIISGGAALSSEVEEFLRVTCCAFFVQGYGLTET 420
           KQK ASPLADFLAFRKVKARLGGRLRLIISGGAALS+EVEEFLRVTCCA FVQGYGLTET
Sbjct: 361 KQKDASPLADFLAFRKVKARLGGRLRLIISGGAALSAEVEEFLRVTCCALFVQGYGLTET 420

Query: 421 CGPTTIGFPDEMCMLGTVGAVTLYNELRLEEVPEMGYNPLGERPCGEICVRGKTVSNEYY 480
           CGPTT+GFPDEMCMLG VGAVTLYNELRLEEVPEMGY+PLG+RPCGEICVRGKTV NEYY
Sbjct: 421 CGPTTVGFPDEMCMLGNVGAVTLYNELRLEEVPEMGYDPLGDRPCGEICVRGKTVFNEYY 480

Query: 481 KNPELTKEAIKDGWFHTGDIGEILPNGVLKIIDRKKNLIKLSQGEYIALEYLENVYSITP 540
           KNPELTKE+IKDGWFHTGDIGEI PNGV+KIIDRKKNLIKLSQGEYIALEYLENVYS+TP
Sbjct: 481 KNPELTKESIKDGWFHTGDIGEIFPNGVVKIIDRKKNLIKLSQGEYIALEYLENVYSVTP 540

Query: 541 IVEDIWIFGNSFKSMLVAVVVLHEENTKKWANSNGFLCSFSELCSLEQLRHYVLSELTST 600
           IVEDIWI+GNSFKS LVAVVVLHEENTKKWANSNGFLCSFSELC LEQLR YVLSELTST
Sbjct: 541 IVEDIWIYGNSFKSKLVAVVVLHEENTKKWANSNGFLCSFSELCCLEQLRQYVLSELTST 600

Query: 601 AERNKLKRFEYIKGVVLESRPFDIEDDLVTATLKKKRNKLLKHYEEEINAEYKNLEANR 660
           AER KLKRFEYIKGVVLESRPFDIED+LVTATLKKKRN L+KHYE+EINAEYKNLEA R
Sbjct: 601 AERTKLKRFEYIKGVVLESRPFDIEDELVTATLKKKRNNLVKHYEKEINAEYKNLEAKR 659

BLAST of Lag0031066 vs. ExPASy TrEMBL
Match: A0A6J1DV89 (long chain acyl-CoA synthetase 1 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111023830 PE=4 SV=1)

HSP 1 Score: 1241.9 bits (3212), Expect = 0.0e+00
Identity = 602/657 (91.63%), Postives = 628/657 (95.59%), Query Frame = 0

Query: 1   MKSFSIKVEEGREGKDGKPSVGPVYRNVLSKDGFPPLYEDLSTSWDLFSVSAKKYAEKQM 60
           MKSFS+KVEEGREGKDGKPS+GPVYRN LSKD FPP Y+DLSTSWDLFSVSAKK+A K+M
Sbjct: 1   MKSFSVKVEEGREGKDGKPSIGPVYRNFLSKDEFPPPYDDLSTSWDLFSVSAKKHAAKRM 60

Query: 61  LGWRKFVEGKPGPYIWKTYKEVYVDVLNIGSALRAAGAEPGSRIGIYGANCPQWIIAMEA 120
           LGWRKFVEGKPGPY+WKTYKEVY DVLNIGSALRA GA+PGSRIGIYGANCPQWIIAMEA
Sbjct: 61  LGWRKFVEGKPGPYVWKTYKEVYGDVLNIGSALRAVGAKPGSRIGIYGANCPQWIIAMEA 120

Query: 121 CNAHSLICVPLYDTLGHGAVNFIIDHAEIDIVFVQDKKAKELLNSESTNPKRLKVVVCFT 180
           CNAHSLICVPLYDTLGHGAVNFIIDHAEIDIVFVQDKK KELLN EST PKRLKVVVCFT
Sbjct: 121 CNAHSLICVPLYDTLGHGAVNFIIDHAEIDIVFVQDKKVKELLNPESTYPKRLKVVVCFT 180

Query: 181 SLKGEEKTSAVGNGMTPYSWDEFLRLGKENPSEITPPKPLDICTIMYTSGTSGTPKGVVL 240
           SLK EEKTSAV  G+ PYSWDEFL  GKENP EI PP+PL ICTIMYTSGTSGTPKGVVL
Sbjct: 181 SLKKEEKTSAVEIGIKPYSWDEFLHSGKENPFEIVPPQPLSICTIMYTSGTSGTPKGVVL 240

Query: 241 THETVTTFIRGVDIYLEQFEDKMTEDDVYLSFLPLAHILDRTVEEYFFRKGASVGYYHGD 300
           THET+TTFIRGVD++LEQF+DKMTEDDVYLSFLPLAHILDR VEEYFFRKGASVGYYHGD
Sbjct: 241 THETITTFIRGVDVFLEQFDDKMTEDDVYLSFLPLAHILDRAVEEYFFRKGASVGYYHGD 300

Query: 301 LNAIKDDLMELKPTLLVGVPRVFERIHEGIKQAVQILNPVRRKAFDLLYKYKLSWLNSGY 360
           LN IK+DLMELKPT+LVGVPRVFERIHEGIKQAVQ LNPVRRKAFDLLYKYKLSWLNSGY
Sbjct: 301 LNEIKEDLMELKPTVLVGVPRVFERIHEGIKQAVQELNPVRRKAFDLLYKYKLSWLNSGY 360

Query: 361 KQKYASPLADFLAFRKVKARLGGRLRLIISGGAALSSEVEEFLRVTCCAFFVQGYGLTET 420
           KQKYASPLADFLAFRKVKARLGGR+RLIISGGAALS+EVEEFLRVTCCAFFVQGYGLTET
Sbjct: 361 KQKYASPLADFLAFRKVKARLGGRIRLIISGGAALSAEVEEFLRVTCCAFFVQGYGLTET 420

Query: 421 CGPTTIGFPDEMCMLGTVGAVTLYNELRLEEVPEMGYNPLGERPCGEICVRGKTVSNEYY 480
           CGPTTIGFPDEMCMLGTVGAVTLYNEL LEEVPEMGYNPLG+ PCGEICVRGKTV NEYY
Sbjct: 421 CGPTTIGFPDEMCMLGTVGAVTLYNELCLEEVPEMGYNPLGDPPCGEICVRGKTVFNEYY 480

Query: 481 KNPELTKEAIKDGWFHTGDIGEILPNGVLKIIDRKKNLIKLSQGEYIALEYLENVYSITP 540
           KNPELTKE+IKDGWFHTGDIGE+LPNGVLKIIDRKKNLIKLSQGEYIALEYLENVYSITP
Sbjct: 481 KNPELTKESIKDGWFHTGDIGEMLPNGVLKIIDRKKNLIKLSQGEYIALEYLENVYSITP 540

Query: 541 IVEDIWIFGNSFKSMLVAVVVLHEENTKKWANSNGFLCSFSELCSLEQLRHYVLSELTST 600
           IVEDIWI+GNSFKSMLVAVVVLHEENTK+WA SNGFLCSFSELCSL+Q+RHYVLSELTST
Sbjct: 541 IVEDIWIYGNSFKSMLVAVVVLHEENTKRWAKSNGFLCSFSELCSLDQVRHYVLSELTST 600

Query: 601 AERNKLKRFEYIKGVVLESRPFDIEDDLVTATLKKKRNKLLKHYEEEINAEYKNLEA 658
           AERNKLKRFEYIKGVVLES PFD+EDDLVTATLKKKRNKLLK+YE EINA YK LEA
Sbjct: 601 AERNKLKRFEYIKGVVLESHPFDVEDDLVTATLKKKRNKLLKYYEVEINAVYKKLEA 657

BLAST of Lag0031066 vs. ExPASy TrEMBL
Match: A0A6J1DS92 (long chain acyl-CoA synthetase 1 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111023830 PE=4 SV=1)

HSP 1 Score: 1241.9 bits (3212), Expect = 0.0e+00
Identity = 602/657 (91.63%), Postives = 628/657 (95.59%), Query Frame = 0

Query: 1   MKSFSIKVEEGREGKDGKPSVGPVYRNVLSKDGFPPLYEDLSTSWDLFSVSAKKYAEKQM 60
           MKSFS+KVEEGREGKDGKPS+GPVYRN LSKD FPP Y+DLSTSWDLFSVSAKK+A K+M
Sbjct: 13  MKSFSVKVEEGREGKDGKPSIGPVYRNFLSKDEFPPPYDDLSTSWDLFSVSAKKHAAKRM 72

Query: 61  LGWRKFVEGKPGPYIWKTYKEVYVDVLNIGSALRAAGAEPGSRIGIYGANCPQWIIAMEA 120
           LGWRKFVEGKPGPY+WKTYKEVY DVLNIGSALRA GA+PGSRIGIYGANCPQWIIAMEA
Sbjct: 73  LGWRKFVEGKPGPYVWKTYKEVYGDVLNIGSALRAVGAKPGSRIGIYGANCPQWIIAMEA 132

Query: 121 CNAHSLICVPLYDTLGHGAVNFIIDHAEIDIVFVQDKKAKELLNSESTNPKRLKVVVCFT 180
           CNAHSLICVPLYDTLGHGAVNFIIDHAEIDIVFVQDKK KELLN EST PKRLKVVVCFT
Sbjct: 133 CNAHSLICVPLYDTLGHGAVNFIIDHAEIDIVFVQDKKVKELLNPESTYPKRLKVVVCFT 192

Query: 181 SLKGEEKTSAVGNGMTPYSWDEFLRLGKENPSEITPPKPLDICTIMYTSGTSGTPKGVVL 240
           SLK EEKTSAV  G+ PYSWDEFL  GKENP EI PP+PL ICTIMYTSGTSGTPKGVVL
Sbjct: 193 SLKKEEKTSAVEIGIKPYSWDEFLHSGKENPFEIVPPQPLSICTIMYTSGTSGTPKGVVL 252

Query: 241 THETVTTFIRGVDIYLEQFEDKMTEDDVYLSFLPLAHILDRTVEEYFFRKGASVGYYHGD 300
           THET+TTFIRGVD++LEQF+DKMTEDDVYLSFLPLAHILDR VEEYFFRKGASVGYYHGD
Sbjct: 253 THETITTFIRGVDVFLEQFDDKMTEDDVYLSFLPLAHILDRAVEEYFFRKGASVGYYHGD 312

Query: 301 LNAIKDDLMELKPTLLVGVPRVFERIHEGIKQAVQILNPVRRKAFDLLYKYKLSWLNSGY 360
           LN IK+DLMELKPT+LVGVPRVFERIHEGIKQAVQ LNPVRRKAFDLLYKYKLSWLNSGY
Sbjct: 313 LNEIKEDLMELKPTVLVGVPRVFERIHEGIKQAVQELNPVRRKAFDLLYKYKLSWLNSGY 372

Query: 361 KQKYASPLADFLAFRKVKARLGGRLRLIISGGAALSSEVEEFLRVTCCAFFVQGYGLTET 420
           KQKYASPLADFLAFRKVKARLGGR+RLIISGGAALS+EVEEFLRVTCCAFFVQGYGLTET
Sbjct: 373 KQKYASPLADFLAFRKVKARLGGRIRLIISGGAALSAEVEEFLRVTCCAFFVQGYGLTET 432

Query: 421 CGPTTIGFPDEMCMLGTVGAVTLYNELRLEEVPEMGYNPLGERPCGEICVRGKTVSNEYY 480
           CGPTTIGFPDEMCMLGTVGAVTLYNEL LEEVPEMGYNPLG+ PCGEICVRGKTV NEYY
Sbjct: 433 CGPTTIGFPDEMCMLGTVGAVTLYNELCLEEVPEMGYNPLGDPPCGEICVRGKTVFNEYY 492

Query: 481 KNPELTKEAIKDGWFHTGDIGEILPNGVLKIIDRKKNLIKLSQGEYIALEYLENVYSITP 540
           KNPELTKE+IKDGWFHTGDIGE+LPNGVLKIIDRKKNLIKLSQGEYIALEYLENVYSITP
Sbjct: 493 KNPELTKESIKDGWFHTGDIGEMLPNGVLKIIDRKKNLIKLSQGEYIALEYLENVYSITP 552

Query: 541 IVEDIWIFGNSFKSMLVAVVVLHEENTKKWANSNGFLCSFSELCSLEQLRHYVLSELTST 600
           IVEDIWI+GNSFKSMLVAVVVLHEENTK+WA SNGFLCSFSELCSL+Q+RHYVLSELTST
Sbjct: 553 IVEDIWIYGNSFKSMLVAVVVLHEENTKRWAKSNGFLCSFSELCSLDQVRHYVLSELTST 612

Query: 601 AERNKLKRFEYIKGVVLESRPFDIEDDLVTATLKKKRNKLLKHYEEEINAEYKNLEA 658
           AERNKLKRFEYIKGVVLES PFD+EDDLVTATLKKKRNKLLK+YE EINA YK LEA
Sbjct: 613 AERNKLKRFEYIKGVVLESHPFDVEDDLVTATLKKKRNKLLKYYEVEINAVYKKLEA 669

BLAST of Lag0031066 vs. ExPASy TrEMBL
Match: A0A1S4DWM9 (long chain acyl-CoA synthetase 1 OS=Cucumis melo OX=3656 GN=LOC103489907 PE=4 SV=1)

HSP 1 Score: 1237.2 bits (3200), Expect = 0.0e+00
Identity = 597/656 (91.01%), Postives = 627/656 (95.58%), Query Frame = 0

Query: 1   MKSFSIKVEEGREGKDGKPSVGPVYRNVLSKDGFPPLYEDLSTSWDLFSVSAKKYAEKQM 60
           MK FS+KVEEG+EGKDG+PSVGPVYRNVL++  FP  YEDLSTSWDLFSVSAKKYA+++M
Sbjct: 1   MKRFSVKVEEGKEGKDGEPSVGPVYRNVLAEKEFPSPYEDLSTSWDLFSVSAKKYADRRM 60

Query: 61  LGWRKFVEGKPGPYIWKTYKEVYVDVLNIGSALRAAGAEPGSRIGIYGANCPQWIIAMEA 120
           LGWRKFVEGKPGPYIWKTYKEVY DVLNIGSALRA GAEPGSRIGIYGANCPQWI+AMEA
Sbjct: 61  LGWRKFVEGKPGPYIWKTYKEVYDDVLNIGSALRAIGAEPGSRIGIYGANCPQWIVAMEA 120

Query: 121 CNAHSLICVPLYDTLGHGAVNFIIDHAEIDIVFVQDKKAKELLNSESTNPKRLKVVVCFT 180
           CNAHSLICVPLYDTLGHGAVNFIIDHAEIDI+F+QDKKA ELLNSE   P+RLKVV+CFT
Sbjct: 121 CNAHSLICVPLYDTLGHGAVNFIIDHAEIDIIFIQDKKAIELLNSECKIPQRLKVVICFT 180

Query: 181 SLKGEEKTSAVGNGMTPYSWDEFLRLGKENPSEITPPKPLDICTIMYTSGTSGTPKGVVL 240
           SLK EEK SA  NGM PYSWDEFLRLGKENPSEI+PP+PLDICTIMYTSGTSGTPKGVVL
Sbjct: 181 SLKEEEKISATNNGMKPYSWDEFLRLGKENPSEISPPRPLDICTIMYTSGTSGTPKGVVL 240

Query: 241 THETVTTFIRGVDIYLEQFEDKMTEDDVYLSFLPLAHILDRTVEEYFFRKGASVGYYHGD 300
           T ET+TTFIRGVDI +EQFEDKMTEDDVYLSFLPLAHILDRTVEEYFFRKGASVGYYHGD
Sbjct: 241 TQETITTFIRGVDIQMEQFEDKMTEDDVYLSFLPLAHILDRTVEEYFFRKGASVGYYHGD 300

Query: 301 LNAIKDDLMELKPTLLVGVPRVFERIHEGIKQAVQILNPVRRKAFDLLYKYKLSWLNSGY 360
           LNAIKDDLMELKPTLLVGVPRVFE+IHEGIKQAVQ+LNPVRRKAFDLLYKYKLSW+NSGY
Sbjct: 301 LNAIKDDLMELKPTLLVGVPRVFEKIHEGIKQAVQVLNPVRRKAFDLLYKYKLSWMNSGY 360

Query: 361 KQKYASPLADFLAFRKVKARLGGRLRLIISGGAALSSEVEEFLRVTCCAFFVQGYGLTET 420
           KQK ASPLADFLAFRKVKARLGGRLRLIISGGAALS+EVEEFLRVTCCAFFVQGYGLTET
Sbjct: 361 KQKDASPLADFLAFRKVKARLGGRLRLIISGGAALSTEVEEFLRVTCCAFFVQGYGLTET 420

Query: 421 CGPTTIGFPDEMCMLGTVGAVTLYNELRLEEVPEMGYNPLGERPCGEICVRGKTVSNEYY 480
           CGPTTIGFPDEMCMLG VG VTL+NELRLEEVP+MGYNPLG+RPCGEICVRGKTV  EYY
Sbjct: 421 CGPTTIGFPDEMCMLGNVGGVTLFNELRLEEVPDMGYNPLGDRPCGEICVRGKTVFTEYY 480

Query: 481 KNPELTKEAIKDGWFHTGDIGEILPNGVLKIIDRKKNLIKLSQGEYIALEYLENVYSITP 540
           K+PELTKE+IKDGWFHTGDIGE+ PNGV+KIIDRKKNLIKLSQGEYIALEYLENVYSITP
Sbjct: 481 KDPELTKESIKDGWFHTGDIGEMFPNGVMKIIDRKKNLIKLSQGEYIALEYLENVYSITP 540

Query: 541 IVEDIWIFGNSFKSMLVAVVVLHEENTKKWANSNGFLCSFSELCSLEQLRHYVLSELTST 600
           IVEDIWI+GNSFKSMLVAVVVLHEENTKKWANSNGFLCSFSELC LEQLRHYVLSELTST
Sbjct: 541 IVEDIWIYGNSFKSMLVAVVVLHEENTKKWANSNGFLCSFSELCPLEQLRHYVLSELTST 600

Query: 601 AERNKLKRFEYIKGVVLESRPFDIEDDLVTATLKKKRNKLLKHYEEEINAEYKNLE 657
           AERNKLKRFEYIKGVVLES PFDIE DLVTATLKKKRN LLKHYE +INAEYKNLE
Sbjct: 601 AERNKLKRFEYIKGVVLESHPFDIEKDLVTATLKKKRNNLLKHYEAQINAEYKNLE 656

BLAST of Lag0031066 vs. TAIR 10
Match: AT2G47240.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 946.8 bits (2446), Expect = 9.2e-276
Identity = 440/659 (66.77%), Postives = 539/659 (81.79%), Query Frame = 0

Query: 1   MKSFSIKVEEGREGKDGKPSVGPVYRNVLSKDGFPPLYEDLSTSWDLFSVSAKKYAEKQM 60
           MKSF+ KVEEG +G DGKPSVGPVYRN+LS+ GFPP+  +++T+WD+FS S +K+ +  M
Sbjct: 1   MKSFAAKVEEGVKGIDGKPSVGPVYRNLLSEKGFPPIDSEITTAWDIFSKSVEKFPDNNM 60

Query: 61  LGWRKFVEGKPGPYIWKTYKEVYVDVLNIGSALRAAGAEPGSRIGIYGANCPQWIIAMEA 120
           LGWR+ V+ K GPY+WKTYKEVY +VL IGSALRAAGAEPGSR+GIYG NCPQWIIAMEA
Sbjct: 61  LGWRRIVDEKVGPYMWKTYKEVYEEVLQIGSALRAAGAEPGSRVGIYGVNCPQWIIAMEA 120

Query: 121 CNAHSLICVPLYDTLGHGAVNFIIDHAEIDIVFVQDKKAKELLNSESTNPKRLKVVVCFT 180
           C AH+LICVPLYDTLG GAV++I++HAEID VFVQD K K LL  +    KRLK +V FT
Sbjct: 121 CAAHTLICVPLYDTLGSGAVDYIVEHAEIDFVFVQDTKIKGLLEPDCKCAKRLKAIVSFT 180

Query: 181 SLKGEEKTSAVGNGMTPYSWDEFLRLGKENPSEITPPKPLDICTIMYTSGTSGTPKGVVL 240
           ++  E    A   G+  YSW +FL +G+E P +  PPK  +ICTIMYTSGTSG PKGVVL
Sbjct: 181 NVSDELSHKASEIGVKTYSWIDFLHMGREKPEDTNPPKAFNICTIMYTSGTSGDPKGVVL 240

Query: 241 THETVTTFIRGVDIYLEQFEDKMTEDDVYLSFLPLAHILDRTVEEYFFRKGASVGYYHGD 300
           TH+ V TF+ G+D+Y++QFEDKMT DDVYLSFLPLAHILDR  EEYFFRKGASVGYYHG+
Sbjct: 241 THQAVATFVVGMDLYMDQFEDKMTHDDVYLSFLPLAHILDRMNEEYFFRKGASVGYYHGN 300

Query: 301 LNAIKDDLMELKPTLLVGVPRVFERIHEGIKQAVQILNPVRRKAFDLLYKYKLSWLNSGY 360
           LN ++DD+ ELKPT L GVPRVFERIHEGI++A+Q LNP RR  F+ LYK+KL+WLN GY
Sbjct: 301 LNVLRDDIQELKPTYLAGVPRVFERIHEGIQKALQELNPRRRFIFNALYKHKLAWLNRGY 360

Query: 361 KQKYASPLADFLAFRKVKARLGGRLRLIISGGAALSSEVEEFLRVTCCAFFVQGYGLTET 420
               ASP+ADF+AFRK++ +LGGR+RL++SGGA LS E+EEFLRVTCC F VQGYGLTET
Sbjct: 361 SHSKASPMADFIAFRKIRDKLGGRIRLLVSGGAPLSPEIEEFLRVTCCCFVVQGYGLTET 420

Query: 421 CGPTTIGFPDEMCMLGTVGAVTLYNELRLEEVPEMGYNPLGERPCGEICVRGKTVSNEYY 480
            G T +GFPDEMCMLGTVG   +YNE+RLEEV EMGY+PLGE P GEIC+RG+ + + YY
Sbjct: 421 LGGTALGFPDEMCMLGTVGIPAVYNEIRLEEVSEMGYDPLGENPAGEICIRGQCMFSGYY 480

Query: 481 KNPELTKEAIKDGWFHTGDIGEILPNGVLKIIDRKKNLIKLSQGEYIALEYLENVYSITP 540
           KNPELT+E +KDGWFHTGDIGEILPNGVLKIIDRKKNLIKLSQGEY+ALE+LEN++    
Sbjct: 481 KNPELTEEVMKDGWFHTGDIGEILPNGVLKIIDRKKNLIKLSQGEYVALEHLENIFGQNS 540

Query: 541 IVEDIWIFGNSFKSMLVAVVVLHEENTKKWANSNGFLCSFSELCSLEQLRHYVLSELTST 600
           +V+DIW++G+SFKSMLVAVVV + E   +WA   GF   F ELCS  +L+ +++SEL ST
Sbjct: 541 VVQDIWVYGDSFKSMLVAVVVPNPETVNRWAKDLGFTKPFEELCSFPELKEHIISELKST 600

Query: 601 AERNKLKRFEYIKGVVLESRPFDIEDDLVTATLKKKRNKLLKHYEEEINAEYKNLEANR 660
           AE+NKL++FEYIK V +E++PFD+E DLVTATLK +RN LLK+Y+ +I+  Y+ L + +
Sbjct: 601 AEKNKLRKFEYIKAVTVETKPFDVERDLVTATLKNRRNNLLKYYQVQIDEMYRKLASKK 659

BLAST of Lag0031066 vs. TAIR 10
Match: AT2G47240.2 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 946.8 bits (2446), Expect = 9.2e-276
Identity = 440/659 (66.77%), Postives = 539/659 (81.79%), Query Frame = 0

Query: 1   MKSFSIKVEEGREGKDGKPSVGPVYRNVLSKDGFPPLYEDLSTSWDLFSVSAKKYAEKQM 60
           MKSF+ KVEEG +G DGKPSVGPVYRN+LS+ GFPP+  +++T+WD+FS S +K+ +  M
Sbjct: 1   MKSFAAKVEEGVKGIDGKPSVGPVYRNLLSEKGFPPIDSEITTAWDIFSKSVEKFPDNNM 60

Query: 61  LGWRKFVEGKPGPYIWKTYKEVYVDVLNIGSALRAAGAEPGSRIGIYGANCPQWIIAMEA 120
           LGWR+ V+ K GPY+WKTYKEVY +VL IGSALRAAGAEPGSR+GIYG NCPQWIIAMEA
Sbjct: 61  LGWRRIVDEKVGPYMWKTYKEVYEEVLQIGSALRAAGAEPGSRVGIYGVNCPQWIIAMEA 120

Query: 121 CNAHSLICVPLYDTLGHGAVNFIIDHAEIDIVFVQDKKAKELLNSESTNPKRLKVVVCFT 180
           C AH+LICVPLYDTLG GAV++I++HAEID VFVQD K K LL  +    KRLK +V FT
Sbjct: 121 CAAHTLICVPLYDTLGSGAVDYIVEHAEIDFVFVQDTKIKGLLEPDCKCAKRLKAIVSFT 180

Query: 181 SLKGEEKTSAVGNGMTPYSWDEFLRLGKENPSEITPPKPLDICTIMYTSGTSGTPKGVVL 240
           ++  E    A   G+  YSW +FL +G+E P +  PPK  +ICTIMYTSGTSG PKGVVL
Sbjct: 181 NVSDELSHKASEIGVKTYSWIDFLHMGREKPEDTNPPKAFNICTIMYTSGTSGDPKGVVL 240

Query: 241 THETVTTFIRGVDIYLEQFEDKMTEDDVYLSFLPLAHILDRTVEEYFFRKGASVGYYHGD 300
           TH+ V TF+ G+D+Y++QFEDKMT DDVYLSFLPLAHILDR  EEYFFRKGASVGYYHG+
Sbjct: 241 THQAVATFVVGMDLYMDQFEDKMTHDDVYLSFLPLAHILDRMNEEYFFRKGASVGYYHGN 300

Query: 301 LNAIKDDLMELKPTLLVGVPRVFERIHEGIKQAVQILNPVRRKAFDLLYKYKLSWLNSGY 360
           LN ++DD+ ELKPT L GVPRVFERIHEGI++A+Q LNP RR  F+ LYK+KL+WLN GY
Sbjct: 301 LNVLRDDIQELKPTYLAGVPRVFERIHEGIQKALQELNPRRRFIFNALYKHKLAWLNRGY 360

Query: 361 KQKYASPLADFLAFRKVKARLGGRLRLIISGGAALSSEVEEFLRVTCCAFFVQGYGLTET 420
               ASP+ADF+AFRK++ +LGGR+RL++SGGA LS E+EEFLRVTCC F VQGYGLTET
Sbjct: 361 SHSKASPMADFIAFRKIRDKLGGRIRLLVSGGAPLSPEIEEFLRVTCCCFVVQGYGLTET 420

Query: 421 CGPTTIGFPDEMCMLGTVGAVTLYNELRLEEVPEMGYNPLGERPCGEICVRGKTVSNEYY 480
            G T +GFPDEMCMLGTVG   +YNE+RLEEV EMGY+PLGE P GEIC+RG+ + + YY
Sbjct: 421 LGGTALGFPDEMCMLGTVGIPAVYNEIRLEEVSEMGYDPLGENPAGEICIRGQCMFSGYY 480

Query: 481 KNPELTKEAIKDGWFHTGDIGEILPNGVLKIIDRKKNLIKLSQGEYIALEYLENVYSITP 540
           KNPELT+E +KDGWFHTGDIGEILPNGVLKIIDRKKNLIKLSQGEY+ALE+LEN++    
Sbjct: 481 KNPELTEEVMKDGWFHTGDIGEILPNGVLKIIDRKKNLIKLSQGEYVALEHLENIFGQNS 540

Query: 541 IVEDIWIFGNSFKSMLVAVVVLHEENTKKWANSNGFLCSFSELCSLEQLRHYVLSELTST 600
           +V+DIW++G+SFKSMLVAVVV + E   +WA   GF   F ELCS  +L+ +++SEL ST
Sbjct: 541 VVQDIWVYGDSFKSMLVAVVVPNPETVNRWAKDLGFTKPFEELCSFPELKEHIISELKST 600

Query: 601 AERNKLKRFEYIKGVVLESRPFDIEDDLVTATLKKKRNKLLKHYEEEINAEYKNLEANR 660
           AE+NKL++FEYIK V +E++PFD+E DLVTATLK +RN LLK+Y+ +I+  Y+ L + +
Sbjct: 601 AEKNKLRKFEYIKAVTVETKPFDVERDLVTATLKNRRNNLLKYYQVQIDEMYRKLASKK 659

BLAST of Lag0031066 vs. TAIR 10
Match: AT4G23850.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 677.2 bits (1746), Expect = 1.4e-194
Identity = 318/656 (48.48%), Postives = 446/656 (67.99%), Query Frame = 0

Query: 2   KSFSIKVEEGREGKDGKPSVGPVYRNVLSKDGFPPLYEDLSTSWDLFSVSAKKYAEKQML 61
           K +  +VEEG+EG DG+PSVGPVYR++ +KDGFP   E + + WD+F +S +KY    ML
Sbjct: 5   KKYIFQVEEGKEGSDGRPSVGPVYRSIFAKDGFPDPIEGMDSCWDVFRMSVEKYPNNPML 64

Query: 62  GWRKFVEGKPGPYIWKTYKEVYVDVLNIGSALRAAGAEPGSRIGIYGANCPQWIIAMEAC 121
           G R+ V+GKPG Y+W+TY+EVY  V+ +G++LR+ G +  ++ GIYGAN P+WII+MEAC
Sbjct: 65  GRREIVDGKPGKYVWQTYQEVYDIVMKLGNSLRSVGVKDEAKCGIYGANSPEWIISMEAC 124

Query: 122 NAHSLICVPLYDTLGHGAVNFIIDHAEIDIVFVQDKKAKELLNSESTNPKRLKVVVCFTS 181
           NAH L CVPLYDTLG  AV FII H+E+ IVFV++KK  EL  +   + + +K VV F  
Sbjct: 125 NAHGLYCVPLYDTLGADAVEFIISHSEVSIVFVEEKKISELFKTCPNSTEYMKTVVSFGG 184

Query: 182 LKGEEKTSAVGNGMTPYSWDEFLRLGKENPSEITPPKPLDICTIMYTSGTSGTPKGVVLT 241
           +  E+K  A   G+  Y+WDEFL+LG+    ++   K  DICTIMYTSGT+G PKGV+++
Sbjct: 185 VSREQKEEAETFGLVIYAWDEFLKLGEGKQYDLPIKKKSDICTIMYTSGTTGDPKGVMIS 244

Query: 242 HETVTTFIRGVDIYLEQFEDKMTEDDVYLSFLPLAHILDRTVEEYFFRKGASVGYYHGDL 301
           +E++ T I GV   L+   + +T  DVYLS+LPLAHI DR +EE F + GA++G++ GD+
Sbjct: 245 NESIVTLIAGVIRLLKSANEALTVKDVYLSYLPLAHIFDRVIEECFIQHGAAIGFWRGDV 304

Query: 302 NAIKDDLMELKPTLLVGVPRVFERIHEGIKQAVQILNPVRRKAFDLLYKYKLSWLNSGYK 361
             + +DL ELKPT+   VPRV +R++ G+++ +     +++  FD  + YK  ++  G  
Sbjct: 305 KLLIEDLAELKPTIFCAVPRVLDRVYSGLQKKLSDGGFLKKFIFDSAFSYKFGYMKKGQS 364

Query: 362 QKYASPLADFLAFRKVKARLGGRLRLIISGGAALSSEVEEFLRVTCCAFFVQGYGLTETC 421
              ASPL D L F KVK  LGG +R+I+SG A L+S VE FLRV  C   +QGYGLTE+C
Sbjct: 365 HVEASPLFDKLVFSKVKQGLGGNVRIILSGAAPLASHVESFLRVVACCHVLQGYGLTESC 424

Query: 422 GPTTIGFPDEMCMLGTVGAVTLYNELRLEEVPEMGYNPLGERPCGEICVRGKTVSNEYYK 481
             T +  PDE+ MLGTVG      ++RLE VPEM Y+ L     GEIC+RGKT+ + YYK
Sbjct: 425 AGTFVSLPDELGMLGTVGPPVPNVDIRLESVPEMEYDALASTARGEICIRGKTLFSGYYK 484

Query: 482 NPELTKEAIKDGWFHTGDIGEILPNGVLKIIDRKKNLIKLSQGEYIALEYLENVYSITPI 541
             +LTKE + DGW HTGD+GE  P+G +KIIDRKKN+ KLSQGEY+A+E +EN+Y     
Sbjct: 485 REDLTKEVLIDGWLHTGDVGEWQPDGSMKIIDRKKNIFKLSQGEYVAVENIENIYGEVQA 544

Query: 542 VEDIWIFGNSFKSMLVAVVVLHEENTKKWANSNGFLCSFSELCSLEQLRHYVLSELTSTA 601
           V+ +W++GNSF+S L+A+   ++   ++WA  NG    +  LC  E+ + ++L EL   A
Sbjct: 545 VDSVWVYGNSFESFLIAIANPNQHILERWAAENGVSGDYDALCQNEKAKEFILGELVKMA 604

Query: 602 ERNKLKRFEYIKGVVLESRPFDIEDDLVTATLKKKRNKLLKHYEEEINAEYKNLEA 658
           +  K+K FE IK + L+  PFD+E DL+T T KKKR +LLK+Y+  I+  YK + A
Sbjct: 605 KEKKMKGFEIIKAIHLDPVPFDMERDLLTPTFKKKRPQLLKYYQSVIDEMYKTINA 660

BLAST of Lag0031066 vs. TAIR 10
Match: AT4G11030.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 673.7 bits (1737), Expect = 1.5e-193
Identity = 322/652 (49.39%), Postives = 437/652 (67.02%), Query Frame = 0

Query: 2   KSFSIKVEEGREGKDGKPSVGPVYRNVLSKDGFPPLYEDLSTSWDLFSVSAKKYAEKQML 61
           K F  +VE  +E  DG PSVGPVYR+  +++GFP   + + + WD+F  + +KY   +ML
Sbjct: 5   KRFIFEVEAAKEATDGNPSVGPVYRSTFAQNGFPNPIDGIQSCWDIFRTAVEKYPNNRML 64

Query: 62  GWRKFVEGKPGPYIWKTYKEVYVDVLNIGSALRAAGAEPGSRIGIYGANCPQWIIAMEAC 121
           G R+   GK G Y+WKTYKEVY  V+ +G++LR+ G + G + GIYG NC +WII+MEAC
Sbjct: 65  GRREISNGKAGKYVWKTYKEVYDIVIKLGNSLRSCGIKEGEKCGIYGINCCEWIISMEAC 124

Query: 122 NAHSLICVPLYDTLGHGAVNFIIDHAEIDIVFVQDKKAKELLNSESTNPKRLKVVVCFTS 181
           NAH L CVPLYDTLG GAV FII HAE+ I FV++KK  EL  +   + K +K VV F  
Sbjct: 125 NAHGLYCVPLYDTLGAGAVEFIISHAEVSIAFVEEKKIPELFKTCPNSTKYMKTVVSFGG 184

Query: 182 LKGEEKTSAVGNGMTPYSWDEFLRLGKENPSEITPPKPLDICTIMYTSGTSGTPKGVVLT 241
           +K E+K  A   G+  +SWDEFL+LG+    E+   KP DICTIMYTSGT+G PKGV+++
Sbjct: 185 VKPEQKEEAEKLGLVIHSWDEFLKLGEGKQYELPIKKPSDICTIMYTSGTTGDPKGVMIS 244

Query: 242 HETVTTFIRGVDIYLEQFEDKMTEDDVYLSFLPLAHILDRTVEEYFFRKGASVGYYHGDL 301
           +E++ T   GV  +L      ++E DVY+S+LPLAH+ DR +EE   + G S+G++ GD+
Sbjct: 245 NESIVTITTGVMHFLGNVNASLSEKDVYISYLPLAHVFDRAIEECIIQVGGSIGFWRGDV 304

Query: 302 NAIKDDLMELKPTLLVGVPRVFERIHEGIKQAVQILNPVRRKAFDLLYKYKLSWLNSGYK 361
             + +DL ELKP++   VPRV +R++ G++Q +      ++K FD+ + YK   +  G  
Sbjct: 305 KLLIEDLGELKPSIFCAVPRVLDRVYTGLQQKLSGGGFFKKKVFDVAFSYKFGNMKKGQS 364

Query: 362 QKYASPLADFLAFRKVKARLGGRLRLIISGGAALSSEVEEFLRVTCCAFFVQGYGLTETC 421
              ASP  D L F KVK  LGG +R+I+SG A L+S +E FLRV  C   +QGYGLTE+C
Sbjct: 365 HVAASPFCDKLVFNKVKQGLGGNVRIILSGAAPLASHIESFLRVVACCNVLQGYGLTESC 424

Query: 422 GPTTIGFPDEMCMLGTVGAVTLYNELRLEEVPEMGYNPLGERPCGEICVRGKTVSNEYYK 481
             T   FPDE+ MLGTVG      ++RLE VPEM Y+ LG  P GEIC+RGKT+ + YYK
Sbjct: 425 AGTFATFPDELDMLGTVGPPVPNVDIRLESVPEMNYDALGSTPRGEICIRGKTLFSGYYK 484

Query: 482 NPELTKEAIKDGWFHTGDIGEILPNGVLKIIDRKKNLIKLSQGEYIALEYLENVYSITPI 541
             +LTKE   DGW HTGD+GE  PNG +KIIDRKKN+ KL+QGEY+A+E LENVYS   +
Sbjct: 485 REDLTKEVFIDGWLHTGDVGEWQPNGSMKIIDRKKNIFKLAQGEYVAVENLENVYSQVEV 544

Query: 542 VEDIWIFGNSFKSMLVAVVVLHEENTKKWANSNGFLCSFSELCSLEQLRHYVLSELTSTA 601
           +E IW++GNSF+S LVA+    ++  ++WA  NG    F+ +C   + + ++L EL  TA
Sbjct: 545 IESIWVYGNSFESFLVAIANPAQQTLERWAVENGVNGDFNSICQNAKAKAFILGELVKTA 604

Query: 602 ERNKLKRFEYIKGVVLESRPFDIEDDLVTATLKKKRNKLLKHYEEEINAEYK 654
           + NKLK FE IK V LE   FD+E DL+T T KKKR +LLK+Y+  I+  YK
Sbjct: 605 KENKLKGFEIIKDVHLEPVAFDMERDLLTPTYKKKRPQLLKYYQNVIHEMYK 656

BLAST of Lag0031066 vs. TAIR 10
Match: AT1G64400.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 667.2 bits (1720), Expect = 1.4e-191
Identity = 315/651 (48.39%), Postives = 446/651 (68.51%), Query Frame = 0

Query: 4   FSIKVEEGREGKD-GKPSVGPVYRNVLSKDGFPPLYEDLSTSWDLFSVSAKKYAEKQMLG 63
           + ++VE+G++G D G PSVGPVYR++ +KDGFP   +DL ++WD+F +S +K     MLG
Sbjct: 6   YIVEVEKGKQGVDGGSPSVGPVYRSIYAKDGFPEPPDDLVSAWDIFRLSVEKSPNNPMLG 65

Query: 64  WRKFVEGKPGPYIWKTYKEVYVDVLNIGSALRAAGAEPGSRIGIYGANCPQWIIAMEACN 123
            R+ V+GK G Y+W+TYKEV+  V+ +G+++R  G   G + GIYGAN P+WII+MEACN
Sbjct: 66  RREIVDGKAGKYVWQTYKEVHNVVIKLGNSIRTIGVGKGDKCGIYGANSPEWIISMEACN 125

Query: 124 AHSLICVPLYDTLGHGAVNFIIDHAEIDIVFVQDKKAKELLNSESTNPKRLKVVVCFTSL 183
           AH L CVPLYDTLG GA+ FII HAE+ + F ++ K  ELL +   + K LK +V F  +
Sbjct: 126 AHGLYCVPLYDTLGAGAIEFIICHAEVSLAFAEENKISELLKTAPKSTKYLKYIVSFGEV 185

Query: 184 KGEEKTSAVGNGMTPYSWDEFLRLGKENPSEITPPKPLDICTIMYTSGTSGTPKGVVLTH 243
              ++  A  + +T YSWD+FL+LG+    E+   +  D+CTIMYTSGT+G PKGV+LT+
Sbjct: 186 TNNQRVEAERHRLTIYSWDQFLKLGEGKHYELPEKRRSDVCTIMYTSGTTGDPKGVLLTN 245

Query: 244 ETVTTFIRGVDIYLEQFEDKMTEDDVYLSFLPLAHILDRTVEEYFFRKGASVGYYHGDLN 303
           E++   + GV   L+  ++++T  DVYLS+LPLAHI DR +EE    + AS+G++ GD+ 
Sbjct: 246 ESIIHLLEGVKKLLKTIDEELTSKDVYLSYLPLAHIFDRVIEELCIYEAASIGFWRGDVK 305

Query: 304 AIKDDLMELKPTLLVGVPRVFERIHEGIKQAVQILNPVRRKAFDLLYKYKLSWLNSGYKQ 363
            + +D+  LKPT+   VPRV ERI+ G++Q +     V++K F+  +KYK   +  G   
Sbjct: 306 ILIEDIAALKPTVFCAVPRVLERIYTGLQQKLSDGGFVKKKLFNFAFKYKHKNMEKGQPH 365

Query: 364 KYASPLADFLAFRKVKARLGGRLRLIISGGAALSSEVEEFLRVTCCAFFVQGYGLTETCG 423
           + ASP+AD + F+KVK  LGG +RLI+SG A L++ +E FLRV  CA  +QGYGLTE+CG
Sbjct: 366 EQASPIADKIVFKKVKEGLGGNVRLILSGAAPLAAHIESFLRVVACAHVLQGYGLTESCG 425

Query: 424 PTTIGFPDEMCMLGTVGAVTLYNELRLEEVPEMGYNPLGERPCGEICVRGKTVSNEYYKN 483
            T +  P+E+ MLGTVG      ++RLE VPEMGY+ L   P GEIC+RGKT+ + YYK 
Sbjct: 426 GTFVSIPNELSMLGTVGPPVPNVDIRLESVPEMGYDALASNPRGEICIRGKTLFSGYYKR 485

Query: 484 PELTKEAIKDGWFHTGDIGEILPNGVLKIIDRKKNLIKLSQGEYIALEYLENVYSITPIV 543
            +LT+E   DGW HTGD+GE  P+G +KIIDRKKN+ KLSQGEY+A+E LEN+YS    +
Sbjct: 486 EDLTQEVFIDGWLHTGDVGEWQPDGAMKIIDRKKNIFKLSQGEYVAVENLENIYSHVAAI 545

Query: 544 EDIWIFGNSFKSMLVAVVVLHEENTKKWANSNGFLCSFSELCSLEQLRHYVLSELTSTAE 603
           E IW++GNS++S LVAVV   +   + WA  +     F  +C  ++ + +VL E    A+
Sbjct: 546 ESIWVYGNSYESYLVAVVCPSKIQIEHWAKEHKVSGDFESICRNQKTKEFVLGEFNRVAK 605

Query: 604 RNKLKRFEYIKGVVLESRPFDIEDDLVTATLKKKRNKLLKHYEEEINAEYK 654
             KLK FE IKGV L++ PFD+E DL+T + K KR +LLK+Y++EI+  YK
Sbjct: 606 DKKLKGFELIKGVHLDTVPFDMERDLITPSYKMKRPQLLKYYQKEIDEMYK 656