Lag0031060 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0031060
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionDEAD-box ATP-dependent RNA helicase
Locationchr11: 4292769 .. 4296494 (+)
RNA-Seq ExpressionLag0031060
SyntenyLag0031060
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCGAAGAGGAAGTTCGGCTTTGAAGGGTTTGGAATCAACCGGCAAACGACTTACAATTTCGAGCGCTCCCAAGCTCCTCAGCGGCTTTATGTTCCTCCGTCGTCGCGAGGCCATGGACACGACAACTACGAAGATACCGACGTTGATAATATAGAATATGATGATAATGAAACGGAGGAGGCGAGTGGTAATGGCGAAGCTGGCGGAGGCGCTGCTGGTGGTGGTGAAGAAGAAGAAATTGATCCTTTAGATGCTTTCATGGAGGGAATTCATGAAGAGATGAGAGCGCCGCCGCCGCCGAAACCCAAGGAGAAGGTTGATAAGTATAGGGACGACGAGGAGGATGACCCAATGGAGAGCTTTCTCAGGGCAAAGAAGGACGTGGGACTGACACTGGCAGCTGATGCGCTTCATGCTGGTTATGATTCGGACGAGGAGGTTTATGCGGCAGCTAAGGCTGTGGATGCAGGGATGGTCGAGTACGACTCTGATGATAATATGCTGATTCTTGAGAAGAAAAAAATCGAACCAATTCCTGCTCTAGATCATAGCTCCATTGATTACGAGCCGTTCAATAAAGATTTCTATGAGGAGAAAGCTTCAATATCAGGTCATTTTTTAGTAGGAAGTTATGTTTTAGTTTTGTTTCTGAAGTTGGTTATATTTCAGTATTTGCTTTTGCACTTTTTCTTATATTTTCTCTTTGGCTTATGTGACTGCCTTTGATGATTTTGTGTTCATGGCCATTAATTTATCTTCTCCAACTTCTTTTAAATAATTGCTGGTTAAATCGAAATGAAAGTGGACCGAAGTTGGTATTTTTTTCTCTAGGGAAGGAAGAACTTTGTGATGTATGGAGACTACGATTTTTTTCTCTCTCTCTCATGGAAAGAGTTATTGGAAAGAGTTGAAGGTTTGCTAAGATATGCAAACTTCATTCCATTTAATTAAATTGGGTGTTTTAGCCTTTTTATTGTTCTTTTGTATGTCTGAGTACGTCTCCACCTTTTATTTTTCTTGCCTTCCCTTGCTTGCAGTTTCCTAGGGTAAAGTTGGTGAGTTTTGGAAAATTTAAATGATTAATAAGGCTCTAAATTTGTTGGGTGTTTTTTATTCTATGCTTCATCTTTAGGGATGAGTGAGGAGGAAGTTTCTGAGTACCGGAAGAGTTTGGCTATTCGTGTGTCGGGTTTTGATGTTCCTAGGCCAATTAAAACATTTGAAGACTGTGGATTTTCCCCTCAACTTATGAATGCTATCAAAAAGCAAGGGTATGAGAAGCCTACTTCTATACAGTGCCAAGCTATGCCGATTGTGCTTTCAGGGAGAGATATCATTGGTATAGCAAAAACAGGTTCCGGTAAAACTGCTGCTTTTGTTCTTCCGATGATTGTTCATATTATGGATCAACCTGAACTTGAAAAAGAAGAGGGTCCTATTGGAGTGATCTGTGCTCCCACCAGGGAACTCGCACACCAAATATACCTAGAGTGTAAAAAGTTTTCAAAAGCACACGGGTTACGTGTCTCTGCTGTATATGGTGGGATGTCTAAATTTGATCAGCTTAAAGAACTCAAAGCTGGATGTGAGATAGTTGTTGCCACTCCTGGCAGACTGATTGATATGATAAAAATGAAAGCTTTGACAATGTCAAAAGCAACATACCTGGTACTTGATGAAGCCGATAGAATGTTTGACCTTGGATTTGAACCCCAAATTCGATCCATTGTTGGTCAGATTCGGCCAGACCGACAGACCTTACTCTTTTCCGCAACAATGCCTCGTAAGATTGAAAAGTTAGCTCGAGAAATTCTCACTGATCCTGTAAGAGTTACAGTAGGAGAGGTTGGGATGGCTAACGAAGATATCACACAGGTTGTTCACGTGCTTCCTTCTGATCTGGAGAAATTGCCTTGGCTTCTTGAGAAGTTACCTGAAATGATTGACGATGGTGATGTGTTGGTGTTTGCTTCTAAAAAAGCCACAGTGGATGAGATTGAATCTCAACTTCAGCAGAAAGGTTTTAAGGTTGCAGCTCTTCATGGTGACAAAGACCAGGCATCTCGAATGGAAACTCTGCAAAAATTTAAATCTGGTGTCTATCATGTTCTTATTGCAACTGATGTTGCTGCCCGTGGGCTTGACATCAAGTCTATCAAGTCTGTAGTGAACTTCGACATTGCAAAAGACATGGACATGCATGTTCATCGTATTGGTAGAACAGGTCGAGCTGGTGACAAAGATGGGAGAGCACACACCCTTATAACCCAGAAAGAGGCTCGCTTTGCTGGTGATTTAGTTAATAGTTTGATTGCTGCTGGCCAGAATGTTTCAGTGGAGCTGATGGATCTTGCAATGAAGGTAATTTTTCGGTATATGCATATTATTAGGTTTACAGTAAGCTAATGACCTATGAAACTATGCTTATAACTTACCGTTTTTTTCCTTCCCAATTTGTTAGTCTGAAATCAGTTTTTCTTCTAATTTTTTTCACACAAATTATGACTTTAACATCTCACATTTTAGTGGCTGCAGAAATGTTTAGAAGACACTTTGCTATGTTGCTATCAAGAATTAGTGTACTTAAAAAACAGGCCTTCTTTAGGAAATGAATAGCGTTATTCTTAATAGCCATGGATTTAAATGTGTATCACTGATAATAGACGGTTCATTGATGGGAGAATTGTTTTCTCCGAAATTGACGATTTTCTATCTGAACATGATCAAGTTCCATCTGATTAATTTGTTTAACAGAAGATGCCTTCTTGGCCTTGATTTATTCTCTGTCCGGCAATGCCTCGTTGTGGGCTTTTGTGGCTGACTCTTGTAGTAATATTCTATTATTATTTATTGTTAACAATTGGGGCTCCCTCTTGTAAGTTTGTTAGGAGCTTTCTACTGTTATTTGTATGTCTGTTTTCTTAGCCTTGCCTTGCTTATTCTTGTTCTTCTTGTGAAGCATAGTTGTTTGAGAAAGTTGCTAGTATTAGTATAACTTTTTGTTGAACCATTTACCTTTGAAATCATTATAGGATGGGCGATTCAGGTCAAAGCGTGATGCAAGGAAAAAAGGAGGTATTATTCAACATTCGACTGCAATTTCTCTTCCAAGCTGTGAATTATCAACGTATTGATAGAACTTACCAATTATGCACATTGTTGTCTTTGTAGGTGGTGGTGGTGGTGGGAAGAAGGGTAAAGGGAGAGGTGGCAATGGTCGAGGCGTACGTGGAGTGGATTTCGGTCTAGGAATCGGTTACAACCCAGAAAGCAGTGGTAGCAGTCCTTCAACAAACAATGTTCAAAGTCGATCAGCAGCTGTAAATTCTTTGAGGACAGGAATGATAGCGCAGTTTAAAAGCAATTTCGTCGCTGCTTCGTCAGCCCCCCCTAGTCAGGGTTCAAACAACAGCTATAGTGTGCCAGCAAACAAAAGGCCCACATTAGCAGGCTTTGTATCGGGTGGTTCGATTGGCGGGGGTGCGAACAGCCCTGCACCTCCTGTGCCAAGAGCGAACTCATACGTTCCGAATGCTGGAGAATACTCGAGCCAAAAGAACTCAGAAAGGTTGAAAAATCTTACCTTCTTTCTGTTTGTTGGAACTTTTACTACTATGTTTGTATACTTTTATCAATATCCTCATTTGGTGCCTCATATGATCTGATTACAGTTCTGGCGATAGGCCTCGAGAAAGGAGGAGACCCTCTGGCTGGGACAGATGA

mRNA sequence

ATGTCGAAGAGGAAGTTCGGCTTTGAAGGGTTTGGAATCAACCGGCAAACGACTTACAATTTCGAGCGCTCCCAAGCTCCTCAGCGGCTTTATGTTCCTCCGTCGTCGCGAGGCCATGGACACGACAACTACGAAGATACCGACGTTGATAATATAGAATATGATGATAATGAAACGGAGGAGGCGAGTGGTAATGGCGAAGCTGGCGGAGGCGCTGCTGGTGGTGGTGAAGAAGAAGAAATTGATCCTTTAGATGCTTTCATGGAGGGAATTCATGAAGAGATGAGAGCGCCGCCGCCGCCGAAACCCAAGGAGAAGGTTGATAAGTATAGGGACGACGAGGAGGATGACCCAATGGAGAGCTTTCTCAGGGCAAAGAAGGACGTGGGACTGACACTGGCAGCTGATGCGCTTCATGCTGGTTATGATTCGGACGAGGAGGTTTATGCGGCAGCTAAGGCTGTGGATGCAGGGATGGTCGAGTACGACTCTGATGATAATATGCTGATTCTTGAGAAGAAAAAAATCGAACCAATTCCTGCTCTAGATCATAGCTCCATTGATTACGAGCCGTTCAATAAAGATTTCTATGAGGAGAAAGCTTCAATATCAGGGATGAGTGAGGAGGAAGTTTCTGAGTACCGGAAGAGTTTGGCTATTCGTGTGTCGGGTTTTGATGTTCCTAGGCCAATTAAAACATTTGAAGACTGTGGATTTTCCCCTCAACTTATGAATGCTATCAAAAAGCAAGGGTATGAGAAGCCTACTTCTATACAGTGCCAAGCTATGCCGATTGTGCTTTCAGGGAGAGATATCATTGGTATAGCAAAAACAGGTTCCGGTAAAACTGCTGCTTTTGTTCTTCCGATGATTGTTCATATTATGGATCAACCTGAACTTGAAAAAGAAGAGGGTCCTATTGGAGTGATCTGTGCTCCCACCAGGGAACTCGCACACCAAATATACCTAGAGTGTAAAAAGTTTTCAAAAGCACACGGGTTACGTGTCTCTGCTGTATATGGTGGGATGTCTAAATTTGATCAGCTTAAAGAACTCAAAGCTGGATGTGAGATAGTTGTTGCCACTCCTGGCAGACTGATTGATATGATAAAAATGAAAGCTTTGACAATGTCAAAAGCAACATACCTGGTACTTGATGAAGCCGATAGAATGTTTGACCTTGGATTTGAACCCCAAATTCGATCCATTGTTGGTCAGATTCGGCCAGACCGACAGACCTTACTCTTTTCCGCAACAATGCCTCGTAAGATTGAAAAGTTAGCTCGAGAAATTCTCACTGATCCTGTAAGAGTTACAGTAGGAGAGGTTGGGATGGCTAACGAAGATATCACACAGGTTGTTCACGTGCTTCCTTCTGATCTGGAGAAATTGCCTTGGCTTCTTGAGAAGTTACCTGAAATGATTGACGATGGTGATGTGTTGGTGTTTGCTTCTAAAAAAGCCACAGTGGATGAGATTGAATCTCAACTTCAGCAGAAAGGTTTTAAGGTTGCAGCTCTTCATGGTGACAAAGACCAGGCATCTCGAATGGAAACTCTGCAAAAATTTAAATCTGGTGTCTATCATGTTCTTATTGCAACTGATGTTGCTGCCCGTGGGCTTGACATCAAGTCTATCAAGTCTGTAGTGAACTTCGACATTGCAAAAGACATGGACATGCATGTTCATCGTATTGGTAGAACAGGTCGAGCTGGTGACAAAGATGGGAGAGCACACACCCTTATAACCCAGAAAGAGGCTCGCTTTGCTGGTGATTTAGTTAATAGTTTGATTGCTGCTGGCCAGAATGTTTCAGTGGAGCTGATGGATCTTGCAATGAAGGATGGGCGATTCAGGTCAAAGCGTGATGCAAGGAAAAAAGGAGGTGGTGGTGGTGGTGGGAAGAAGGGTAAAGGGAGAGGTGGCAATGGTCGAGGCGTACGTGGAGTGGATTTCGGTCTAGGAATCGGTTACAACCCAGAAAGCAGTGGTAGCAGTCCTTCAACAAACAATGTTCAAAGTCGATCAGCAGCTGTAAATTCTTTGAGGACAGGAATGATAGCGCAGTTTAAAAGCAATTTCGTCGCTGCTTCGTCAGCCCCCCCTAGTCAGGGTTCAAACAACAGCTATAGTGTGCCAGCAAACAAAAGGCCCACATTAGCAGGCTTTGTATCGGGTGGTTCGATTGGCGGGGGTGCGAACAGCCCTGCACCTCCTGTGCCAAGAGCGAACTCATACGTTCCGAATGCTGGAGAATACTCGAGCCAAAAGAACTCAGAAAGTTCTGGCGATAGGCCTCGAGAAAGGAGGAGACCCTCTGGCTGGGACAGATGA

Coding sequence (CDS)

ATGTCGAAGAGGAAGTTCGGCTTTGAAGGGTTTGGAATCAACCGGCAAACGACTTACAATTTCGAGCGCTCCCAAGCTCCTCAGCGGCTTTATGTTCCTCCGTCGTCGCGAGGCCATGGACACGACAACTACGAAGATACCGACGTTGATAATATAGAATATGATGATAATGAAACGGAGGAGGCGAGTGGTAATGGCGAAGCTGGCGGAGGCGCTGCTGGTGGTGGTGAAGAAGAAGAAATTGATCCTTTAGATGCTTTCATGGAGGGAATTCATGAAGAGATGAGAGCGCCGCCGCCGCCGAAACCCAAGGAGAAGGTTGATAAGTATAGGGACGACGAGGAGGATGACCCAATGGAGAGCTTTCTCAGGGCAAAGAAGGACGTGGGACTGACACTGGCAGCTGATGCGCTTCATGCTGGTTATGATTCGGACGAGGAGGTTTATGCGGCAGCTAAGGCTGTGGATGCAGGGATGGTCGAGTACGACTCTGATGATAATATGCTGATTCTTGAGAAGAAAAAAATCGAACCAATTCCTGCTCTAGATCATAGCTCCATTGATTACGAGCCGTTCAATAAAGATTTCTATGAGGAGAAAGCTTCAATATCAGGGATGAGTGAGGAGGAAGTTTCTGAGTACCGGAAGAGTTTGGCTATTCGTGTGTCGGGTTTTGATGTTCCTAGGCCAATTAAAACATTTGAAGACTGTGGATTTTCCCCTCAACTTATGAATGCTATCAAAAAGCAAGGGTATGAGAAGCCTACTTCTATACAGTGCCAAGCTATGCCGATTGTGCTTTCAGGGAGAGATATCATTGGTATAGCAAAAACAGGTTCCGGTAAAACTGCTGCTTTTGTTCTTCCGATGATTGTTCATATTATGGATCAACCTGAACTTGAAAAAGAAGAGGGTCCTATTGGAGTGATCTGTGCTCCCACCAGGGAACTCGCACACCAAATATACCTAGAGTGTAAAAAGTTTTCAAAAGCACACGGGTTACGTGTCTCTGCTGTATATGGTGGGATGTCTAAATTTGATCAGCTTAAAGAACTCAAAGCTGGATGTGAGATAGTTGTTGCCACTCCTGGCAGACTGATTGATATGATAAAAATGAAAGCTTTGACAATGTCAAAAGCAACATACCTGGTACTTGATGAAGCCGATAGAATGTTTGACCTTGGATTTGAACCCCAAATTCGATCCATTGTTGGTCAGATTCGGCCAGACCGACAGACCTTACTCTTTTCCGCAACAATGCCTCGTAAGATTGAAAAGTTAGCTCGAGAAATTCTCACTGATCCTGTAAGAGTTACAGTAGGAGAGGTTGGGATGGCTAACGAAGATATCACACAGGTTGTTCACGTGCTTCCTTCTGATCTGGAGAAATTGCCTTGGCTTCTTGAGAAGTTACCTGAAATGATTGACGATGGTGATGTGTTGGTGTTTGCTTCTAAAAAAGCCACAGTGGATGAGATTGAATCTCAACTTCAGCAGAAAGGTTTTAAGGTTGCAGCTCTTCATGGTGACAAAGACCAGGCATCTCGAATGGAAACTCTGCAAAAATTTAAATCTGGTGTCTATCATGTTCTTATTGCAACTGATGTTGCTGCCCGTGGGCTTGACATCAAGTCTATCAAGTCTGTAGTGAACTTCGACATTGCAAAAGACATGGACATGCATGTTCATCGTATTGGTAGAACAGGTCGAGCTGGTGACAAAGATGGGAGAGCACACACCCTTATAACCCAGAAAGAGGCTCGCTTTGCTGGTGATTTAGTTAATAGTTTGATTGCTGCTGGCCAGAATGTTTCAGTGGAGCTGATGGATCTTGCAATGAAGGATGGGCGATTCAGGTCAAAGCGTGATGCAAGGAAAAAAGGAGGTGGTGGTGGTGGTGGGAAGAAGGGTAAAGGGAGAGGTGGCAATGGTCGAGGCGTACGTGGAGTGGATTTCGGTCTAGGAATCGGTTACAACCCAGAAAGCAGTGGTAGCAGTCCTTCAACAAACAATGTTCAAAGTCGATCAGCAGCTGTAAATTCTTTGAGGACAGGAATGATAGCGCAGTTTAAAAGCAATTTCGTCGCTGCTTCGTCAGCCCCCCCTAGTCAGGGTTCAAACAACAGCTATAGTGTGCCAGCAAACAAAAGGCCCACATTAGCAGGCTTTGTATCGGGTGGTTCGATTGGCGGGGGTGCGAACAGCCCTGCACCTCCTGTGCCAAGAGCGAACTCATACGTTCCGAATGCTGGAGAATACTCGAGCCAAAAGAACTCAGAAAGTTCTGGCGATAGGCCTCGAGAAAGGAGGAGACCCTCTGGCTGGGACAGATGA

Protein sequence

MSKRKFGFEGFGINRQTTYNFERSQAPQRLYVPPSSRGHGHDNYEDTDVDNIEYDDNETEEASGNGEAGGGAAGGGEEEEIDPLDAFMEGIHEEMRAPPPPKPKEKVDKYRDDEEDDPMESFLRAKKDVGLTLAADALHAGYDSDEEVYAAAKAVDAGMVEYDSDDNMLILEKKKIEPIPALDHSSIDYEPFNKDFYEEKASISGMSEEEVSEYRKSLAIRVSGFDVPRPIKTFEDCGFSPQLMNAIKKQGYEKPTSIQCQAMPIVLSGRDIIGIAKTGSGKTAAFVLPMIVHIMDQPELEKEEGPIGVICAPTRELAHQIYLECKKFSKAHGLRVSAVYGGMSKFDQLKELKAGCEIVVATPGRLIDMIKMKALTMSKATYLVLDEADRMFDLGFEPQIRSIVGQIRPDRQTLLFSATMPRKIEKLAREILTDPVRVTVGEVGMANEDITQVVHVLPSDLEKLPWLLEKLPEMIDDGDVLVFASKKATVDEIESQLQQKGFKVAALHGDKDQASRMETLQKFKSGVYHVLIATDVAARGLDIKSIKSVVNFDIAKDMDMHVHRIGRTGRAGDKDGRAHTLITQKEARFAGDLVNSLIAAGQNVSVELMDLAMKDGRFRSKRDARKKGGGGGGGKKGKGRGGNGRGVRGVDFGLGIGYNPESSGSSPSTNNVQSRSAAVNSLRTGMIAQFKSNFVAASSAPPSQGSNNSYSVPANKRPTLAGFVSGGSIGGGANSPAPPVPRANSYVPNAGEYSSQKNSESSGDRPRERRRPSGWDR
Homology
BLAST of Lag0031060 vs. NCBI nr
Match: XP_008454276.1 (PREDICTED: DEAD-box ATP-dependent RNA helicase 24 [Cucumis melo])

HSP 1 Score: 1436.8 bits (3718), Expect = 0.0e+00
Identity = 743/777 (95.62%), Postives = 760/777 (97.81%), Query Frame = 0

Query: 1   MSKRKFGFEGFGINRQTTYNFERSQAPQRLYVPPSSRGHGHDNYEDTDVDNIEYDDNETE 60
           MSKRKFGFEGFGINRQTTYNFERSQAPQRLYVPPSSRGHGHDNYEDTDVDNIEYDDN+ E
Sbjct: 1   MSKRKFGFEGFGINRQTTYNFERSQAPQRLYVPPSSRGHGHDNYEDTDVDNIEYDDNDGE 60

Query: 61  EASGNGEAGGGAAGGGEEEEIDPLDAFMEGIHEEMRAPPPPKPKEKVDKYRDDEEDDPME 120
           EASGN + G GAA G EEEEIDPLDAFMEGIHEEM+APPPPKPKEKVDKYRDDEEDDPME
Sbjct: 61  EASGNDDGGAGAA-GAEEEEIDPLDAFMEGIHEEMKAPPPPKPKEKVDKYRDDEEDDPME 120

Query: 121 SFLRAKKDVGLTLAADALHAGYDSDEEVYAAAKAVDAGMVEYDSDDNMLILEKKKIEPIP 180
           SFLRAKKDVGLTLAADALHAGYDSDEEVYAAAKAVDAGMVEYDSDDNMLI+EKKKIEPIP
Sbjct: 121 SFLRAKKDVGLTLAADALHAGYDSDEEVYAAAKAVDAGMVEYDSDDNMLIVEKKKIEPIP 180

Query: 181 ALDHSSIDYEPFNKDFYEEKASISGMSEEEVSEYRKSLAIRVSGFDVPRPIKTFEDCGFS 240
           ALDHSSIDYEPFNKDFYEEKASISGMSEEEVSEYRKSLAIRVSGFDVPRPIKTFEDCGFS
Sbjct: 181 ALDHSSIDYEPFNKDFYEEKASISGMSEEEVSEYRKSLAIRVSGFDVPRPIKTFEDCGFS 240

Query: 241 PQLMNAIKKQGYEKPTSIQCQAMPIVLSGRDIIGIAKTGSGKTAAFVLPMIVHIMDQPEL 300
           PQLMNAIKKQGYEKPTSIQCQAMPIVLSG DIIGIAKTGSGKTAAFVLPMIVHIMDQPEL
Sbjct: 241 PQLMNAIKKQGYEKPTSIQCQAMPIVLSGGDIIGIAKTGSGKTAAFVLPMIVHIMDQPEL 300

Query: 301 EKEEGPIGVICAPTRELAHQIYLECKKFSKAHGLRVSAVYGGMSKFDQLKELKAGCEIVV 360
           EKEEGPIGVICAPTRELAHQIYLECKKFSKAHGLRVSAVYGGMSKFDQLKELKAGCEIVV
Sbjct: 301 EKEEGPIGVICAPTRELAHQIYLECKKFSKAHGLRVSAVYGGMSKFDQLKELKAGCEIVV 360

Query: 361 ATPGRLIDMIKMKALTMSKATYLVLDEADRMFDLGFEPQIRSIVGQIRPDRQTLLFSATM 420
           ATPGRLIDMIK+KALTMSKATYLVLDEADRMFDLGFEPQIRSIVGQIRPDRQTLLFSATM
Sbjct: 361 ATPGRLIDMIKLKALTMSKATYLVLDEADRMFDLGFEPQIRSIVGQIRPDRQTLLFSATM 420

Query: 421 PRKIEKLAREILTDPVRVTVGEVGMANEDITQVVHVLPSDLEKLPWLLEKLPEMIDDGDV 480
           PRK+EKLAREILTDPVRVTVGEVGMANEDITQVVHVLPSDLEKLPWLLEKLPEMIDDGDV
Sbjct: 421 PRKVEKLAREILTDPVRVTVGEVGMANEDITQVVHVLPSDLEKLPWLLEKLPEMIDDGDV 480

Query: 481 LVFASKKATVDEIESQLQQKGFKVAALHGDKDQASRMETLQKFKSGVYHVLIATDVAARG 540
           LVFASKKATVDE+ESQL QKGFKVAALHGDKDQASRMETLQKFKSGVYHVLIATDVAARG
Sbjct: 481 LVFASKKATVDEVESQLLQKGFKVAALHGDKDQASRMETLQKFKSGVYHVLIATDVAARG 540

Query: 541 LDIKSIKSVVNFDIAKDMDMHVHRIGRTGRAGDKDGRAHTLITQKEARFAGDLVNSLIAA 600
           LDIKSIKSVVNFDIAKDMDMHVHRIGRTGRAGDKDGRAHTLITQKEARFAG+LVNSLIAA
Sbjct: 541 LDIKSIKSVVNFDIAKDMDMHVHRIGRTGRAGDKDGRAHTLITQKEARFAGELVNSLIAA 600

Query: 601 GQNVSVELMDLAMKDGRFRSKRDARKKGGGGGGGKKGKGRGGNGRGVRGVDFGLGIGYNP 660
           GQNVSVELMDLAMKDGRFRSKRDARKKGGGGGGGKKGK RG NGRGVRGVDFGLGIGYNP
Sbjct: 601 GQNVSVELMDLAMKDGRFRSKRDARKKGGGGGGGKKGKSRGSNGRGVRGVDFGLGIGYNP 660

Query: 661 ESSGSSPSTNNVQSRSAAVNSLRTGMIAQFKSNFVAASSAPPSQGSNNSYSVPANKRPTL 720
           E SGS+PST NVQSRSAAVNSLRTGM+AQFKSNFVAASSAPPSQGS+NSY++PANKRPTL
Sbjct: 661 EGSGSTPSTTNVQSRSAAVNSLRTGMMAQFKSNFVAASSAPPSQGSSNSYNMPANKRPTL 720

Query: 721 AGFVSGGSIGGGANSPAPPVPRANSYVPNAGEYSSQKNSESSGDRPRERRRPSGWDR 778
           +GFVSGGSIGGGANSPAPPV R NSY+PN  EYSSQKN+ESS DRPRER+RPSGWDR
Sbjct: 721 SGFVSGGSIGGGANSPAPPVSRGNSYMPNPVEYSSQKNTESSSDRPRERKRPSGWDR 776

BLAST of Lag0031060 vs. NCBI nr
Match: XP_022948807.1 (DEAD-box ATP-dependent RNA helicase 24 isoform X2 [Cucurbita moschata])

HSP 1 Score: 1431.4 bits (3704), Expect = 0.0e+00
Identity = 743/778 (95.50%), Postives = 762/778 (97.94%), Query Frame = 0

Query: 1   MSKRKFGFEGFGINRQTTYNFERSQAPQRLYVPPSSRGHGHDNYEDTDVDNIEYDDNETE 60
           MSKRKFGFEGFGINRQTTYNFERSQAPQRLYVPPSSRGHGHDNYEDTDVDNIEYDDNE E
Sbjct: 1   MSKRKFGFEGFGINRQTTYNFERSQAPQRLYVPPSSRGHGHDNYEDTDVDNIEYDDNEAE 60

Query: 61  EASGNGEAGGGAAGGGEEEEIDPLDAFMEGIHEEMRAPPPPKPKEKVDKYRDDEEDDPME 120
           EASGNG+   G AGGGEEEEIDPLDAFMEGIHEEMRAPPPPKPKEKVDKYRD+EEDDPME
Sbjct: 61  EASGNGDNVAG-AGGGEEEEIDPLDAFMEGIHEEMRAPPPPKPKEKVDKYRDEEEDDPME 120

Query: 121 SFLRAKKDVGLTLAADALHAGYDSDEEVYAAAKAVDAGMVEYDSDDNMLILEKKKIEPIP 180
           SFL+AKKDVGLTLAADALHAGYDSDEEVYAAAKAVDA MVEYDSDDNMLI+EKKKIE IP
Sbjct: 121 SFLKAKKDVGLTLAADALHAGYDSDEEVYAAAKAVDAAMVEYDSDDNMLIVEKKKIESIP 180

Query: 181 ALDHSSIDYEPFNKDFYEEKASISGMSEEEVSEYRKSLAIRVSGFDVPRPIKTFEDCGFS 240
           AL+HSSIDYEPFNKDFYEEKASISGMSEEEVSEYRK+LAIRVSGFDVPRPIKTFED GFS
Sbjct: 181 ALEHSSIDYEPFNKDFYEEKASISGMSEEEVSEYRKNLAIRVSGFDVPRPIKTFEDSGFS 240

Query: 241 PQLMNAIKKQGYEKPTSIQCQAMPIVLSGRDIIGIAKTGSGKTAAFVLPMIVHIMDQPEL 300
           PQLMNAIKKQGYEKPTSIQCQAMPIVLSGRDIIGIAKTGSGKTAAFVLPMIVHIMDQPEL
Sbjct: 241 PQLMNAIKKQGYEKPTSIQCQAMPIVLSGRDIIGIAKTGSGKTAAFVLPMIVHIMDQPEL 300

Query: 301 EKEEGPIGVICAPTRELAHQIYLECKKFSKAHGLRVSAVYGGMSKFDQLKELKAGCEIVV 360
           EKEEGPIGVICAPTRELAHQIYLECKKFSK HGLRVSAVYGGMSKFDQ+KELKAGCEIVV
Sbjct: 301 EKEEGPIGVICAPTRELAHQIYLECKKFSKVHGLRVSAVYGGMSKFDQIKELKAGCEIVV 360

Query: 361 ATPGRLIDMIKMKALTMSKATYLVLDEADRMFDLGFEPQIRSIVGQIRPDRQTLLFSATM 420
           ATPGRLIDMIKMKALTMSKATYLVLDEADRMFDLGFEPQIRSIVGQIRPDRQTLLFSATM
Sbjct: 361 ATPGRLIDMIKMKALTMSKATYLVLDEADRMFDLGFEPQIRSIVGQIRPDRQTLLFSATM 420

Query: 421 PRKIEKLAREILTDPVRVTVGEVGMANEDITQVVHVLPSDLEKLPWLLEKLPEMIDDGDV 480
           PRK+EKLAREILTDPVRVTVGEVGMANEDITQVVHVLPSDLEKLPWLLEKLPEMIDDGDV
Sbjct: 421 PRKVEKLAREILTDPVRVTVGEVGMANEDITQVVHVLPSDLEKLPWLLEKLPEMIDDGDV 480

Query: 481 LVFASKKATVDEIESQLQQKGFKVAALHGDKDQASRMETLQKFKSGVYHVLIATDVAARG 540
           LVFASKKATVDEIESQL QKGFKVAALHGDKDQASRMETLQKFKSGVYH LIATDVAARG
Sbjct: 481 LVFASKKATVDEIESQLLQKGFKVAALHGDKDQASRMETLQKFKSGVYHALIATDVAARG 540

Query: 541 LDIKSIKSVVNFDIAKDMDMHVHRIGRTGRAGDKDGRAHTLITQKEARFAGDLVNSLIAA 600
           LDIKSIKSVVNFDIAKDMDMHVHRIGRTGRAGD+DG+AHTLITQKEARFAG+LVNSLIAA
Sbjct: 541 LDIKSIKSVVNFDIAKDMDMHVHRIGRTGRAGDRDGKAHTLITQKEARFAGELVNSLIAA 600

Query: 601 GQNVSVELMDLAMKDGRFRSKRDARKKGGGGGGGKKGKGR-GGNGRGVRGVDFGLGIGYN 660
           GQNVS+ELMDLAMKDGRFRSKRDARKKGGGGGGGKKGKGR GGNGRGVRGVDFGLGIGYN
Sbjct: 601 GQNVSMELMDLAMKDGRFRSKRDARKKGGGGGGGKKGKGRGGGNGRGVRGVDFGLGIGYN 660

Query: 661 PESSGSSPSTNNVQSRSAAVNSLRTGMIAQFKSNFVAASSAPPSQGSNNSYSVPANKRPT 720
           PESSGS+PSTNNVQSRSAAVNSLRTGM+AQFKS+FVAASSAPPSQGSN+SYS+PANKRPT
Sbjct: 661 PESSGSNPSTNNVQSRSAAVNSLRTGMMAQFKSSFVAASSAPPSQGSNSSYSMPANKRPT 720

Query: 721 LAGFVSGGSIGGGANSPAPPVPRANSYVPNAGEYSSQKNSESSGDRPRERRRPSGWDR 778
           LAGFVSGGSIGGGAN P PP PRANSYVPNAGE+SSQKNSESSGDR +ERRRPSGWDR
Sbjct: 721 LAGFVSGGSIGGGANRPPPPPPRANSYVPNAGEFSSQKNSESSGDRSKERRRPSGWDR 777

BLAST of Lag0031060 vs. NCBI nr
Match: XP_023523273.1 (DEAD-box ATP-dependent RNA helicase 24 isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1428.7 bits (3697), Expect = 0.0e+00
Identity = 746/783 (95.27%), Postives = 764/783 (97.57%), Query Frame = 0

Query: 1   MSKRKFGFEGFGINRQTTYNFERSQAPQRLYVPPSSRGHGHDNYEDTDVDNIEYDDNETE 60
           MSKRKFGFEGFGINRQTTYNFERSQAPQRLYVPPSSRGHGHDNYEDTDVDNIEYDDNE E
Sbjct: 1   MSKRKFGFEGFGINRQTTYNFERSQAPQRLYVPPSSRGHGHDNYEDTDVDNIEYDDNEAE 60

Query: 61  EASGNGE--AGGGA-AGGGEEEEIDPLDAFMEGIHEEMRAPPPPKPKEKVDKYRDDEEDD 120
           EASGNG+  AG GA AGGGEEEEIDPLDAFMEGIHEEMRAPPPPKPKEKVDKYRDDEEDD
Sbjct: 61  EASGNGDNVAGAGAGAGGGEEEEIDPLDAFMEGIHEEMRAPPPPKPKEKVDKYRDDEEDD 120

Query: 121 PMESFLRAKKDVGLTLAADALHAGYDSDEEVYAAAKAVDAGMVEYDSDDNMLILEKKKIE 180
           PMESFL+AKKDVGLTLAADALHAGYDSDEEVYAAAKAVDA MVEYDSDDNMLI+EKKKIE
Sbjct: 121 PMESFLKAKKDVGLTLAADALHAGYDSDEEVYAAAKAVDAAMVEYDSDDNMLIVEKKKIE 180

Query: 181 PIPALDHSSIDYEPFNKDFYEEKASISGMSEEEVSEYRKSLAIRVSGFDVPRPIKTFEDC 240
            IPAL+HSSIDYEPFNKDFYEEKASISGMSEEEVSEYRK+LAIRVSGFDVPRPIKTFED 
Sbjct: 181 SIPALEHSSIDYEPFNKDFYEEKASISGMSEEEVSEYRKNLAIRVSGFDVPRPIKTFEDS 240

Query: 241 GFSPQLMNAIKKQGYEKPTSIQCQAMPIVLSGRDIIGIAKTGSGKTAAFVLPMIVHIMDQ 300
           GFSPQLMNAIKKQGYEKPTSIQCQAMPIVLSGRDIIGIAKTGSGKTAAFVLPMIVHIMDQ
Sbjct: 241 GFSPQLMNAIKKQGYEKPTSIQCQAMPIVLSGRDIIGIAKTGSGKTAAFVLPMIVHIMDQ 300

Query: 301 PELEKEEGPIGVICAPTRELAHQIYLECKKFSKAHGLRVSAVYGGMSKFDQLKELKAGCE 360
           PELEKEEGPIGVICAPTRELAHQIYLECKKFSK HGLRVSAVYGGMSKFDQ+KELKAGCE
Sbjct: 301 PELEKEEGPIGVICAPTRELAHQIYLECKKFSKVHGLRVSAVYGGMSKFDQIKELKAGCE 360

Query: 361 IVVATPGRLIDMIKMKALTMSKATYLVLDEADRMFDLGFEPQIRSIVGQIRPDRQTLLFS 420
           IVVATPGRLIDMIKMKALTMSKATYLVLDEADRMFDLGFEPQIRSIVGQIRPDRQTLLFS
Sbjct: 361 IVVATPGRLIDMIKMKALTMSKATYLVLDEADRMFDLGFEPQIRSIVGQIRPDRQTLLFS 420

Query: 421 ATMPRKIEKLAREILTDPVRVTVGEVGMANEDITQVVHVLPSDLEKLPWLLEKLPEMIDD 480
           ATMPRK+EKLAREILTDPVRVTVGEVGMANEDITQVVHVLPSDLEKLPWLLEKLPEMIDD
Sbjct: 421 ATMPRKVEKLAREILTDPVRVTVGEVGMANEDITQVVHVLPSDLEKLPWLLEKLPEMIDD 480

Query: 481 GDVLVFASKKATVDEIESQLQQKGFKVAALHGDKDQASRMETLQKFKSGVYHVLIATDVA 540
           GDVLVFASKKATVDEIESQL QKGFKVAALHGDKDQASRMETLQKFKSGVYH LIATDVA
Sbjct: 481 GDVLVFASKKATVDEIESQLLQKGFKVAALHGDKDQASRMETLQKFKSGVYHALIATDVA 540

Query: 541 ARGLDIKSIKSVVNFDIAKDMDMHVHRIGRTGRAGDKDGRAHTLITQKEARFAGDLVNSL 600
           ARGLDIKSIKSVVNFDIAKDMDMHVHRIGRTGRAGD+DG+AHTLITQKEARFAG+LVNSL
Sbjct: 541 ARGLDIKSIKSVVNFDIAKDMDMHVHRIGRTGRAGDRDGKAHTLITQKEARFAGELVNSL 600

Query: 601 IAAGQNVSVELMDLAMKDGRFRSKRDARKK--GGGGGGGKKGKGRGG-NGRGVRGVDFGL 660
           IAAGQNVS+ELMDLAMKDGRFRSKRDARKK  GGGGGGGKKGKGRGG NGRGVRGVDFGL
Sbjct: 601 IAAGQNVSMELMDLAMKDGRFRSKRDARKKGGGGGGGGGKKGKGRGGSNGRGVRGVDFGL 660

Query: 661 GIGYNPESSGSSPSTNNVQSRSAAVNSLRTGMIAQFKSNFVAASSAPPSQGSNNSYSVPA 720
           GIGYNPESSGS+PSTNNVQSRSAAVNSLRTGM+AQFKS+FVAASSAPPSQGSN+ YS+PA
Sbjct: 661 GIGYNPESSGSNPSTNNVQSRSAAVNSLRTGMMAQFKSSFVAASSAPPSQGSNSGYSMPA 720

Query: 721 NKRPTLAGFVSGGSIGGGANSPAPPVPRANSYVPNAGEYSSQKNSESSGDRPRERRRPSG 778
           NKRPTLAGFVSGGSIGGGAN P PP PRANSYVPNAGE+SSQKNSESSGDR +ERRRPSG
Sbjct: 721 NKRPTLAGFVSGGSIGGGANRPPPPPPRANSYVPNAGEFSSQKNSESSGDRSKERRRPSG 780

BLAST of Lag0031060 vs. NCBI nr
Match: XP_023523272.1 (DEAD-box ATP-dependent RNA helicase 24 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1427.9 bits (3695), Expect = 0.0e+00
Identity = 746/785 (95.03%), Postives = 764/785 (97.32%), Query Frame = 0

Query: 1   MSKRKFGFEGFGINRQTTYNFERSQAPQRLYVPPSSRGHGHDNYEDTDVDNIEYDDNETE 60
           MSKRKFGFEGFGINRQTTYNFERSQAPQRLYVPPSSRGHGHDNYEDTDVDNIEYDDNE E
Sbjct: 1   MSKRKFGFEGFGINRQTTYNFERSQAPQRLYVPPSSRGHGHDNYEDTDVDNIEYDDNEAE 60

Query: 61  EASGNGE--AGGGA-AGGGEEEEIDPLDAFMEGIHEEMRAPPPPKPKEKVDKYRDDEEDD 120
           EASGNG+  AG GA AGGGEEEEIDPLDAFMEGIHEEMRAPPPPKPKEKVDKYRDDEEDD
Sbjct: 61  EASGNGDNVAGAGAGAGGGEEEEIDPLDAFMEGIHEEMRAPPPPKPKEKVDKYRDDEEDD 120

Query: 121 PMESFLRAKKDVGLTLAADALHAGYDSDEEVYAAAKAVDAGMVEYDSDDNMLILEKKKIE 180
           PMESFL+AKKDVGLTLAADALHAGYDSDEEVYAAAKAVDA MVEYDSDDNMLI+EKKKIE
Sbjct: 121 PMESFLKAKKDVGLTLAADALHAGYDSDEEVYAAAKAVDAAMVEYDSDDNMLIVEKKKIE 180

Query: 181 PIPALDHSSIDYEPFNKDFYEEKASISGMSEEEVSEYRKSLAIRVSGFDVPRPIKTFEDC 240
            IPAL+HSSIDYEPFNKDFYEEKASISGMSEEEVSEYRK+LAIRVSGFDVPRPIKTFED 
Sbjct: 181 SIPALEHSSIDYEPFNKDFYEEKASISGMSEEEVSEYRKNLAIRVSGFDVPRPIKTFEDS 240

Query: 241 GFSPQLMNAIKKQGYEKPTSIQCQAMPIVLSGRDIIGIAKTGSGKTAAFVLPMIVHIMDQ 300
           GFSPQLMNAIKKQGYEKPTSIQCQAMPIVLSGRDIIGIAKTGSGKTAAFVLPMIVHIMDQ
Sbjct: 241 GFSPQLMNAIKKQGYEKPTSIQCQAMPIVLSGRDIIGIAKTGSGKTAAFVLPMIVHIMDQ 300

Query: 301 PELEKEEGPIGVICAPTRELAHQIYLECKKFSKAHGLRVSAVYGGMSKFDQLKELKAGCE 360
           PELEKEEGPIGVICAPTRELAHQIYLECKKFSK HGLRVSAVYGGMSKFDQ+KELKAGCE
Sbjct: 301 PELEKEEGPIGVICAPTRELAHQIYLECKKFSKVHGLRVSAVYGGMSKFDQIKELKAGCE 360

Query: 361 IVVATPGRLIDMIKMKALTMSKATYLVLDEADRMFDLGFEPQIRSIVGQIRPDRQTLLFS 420
           IVVATPGRLIDMIKMKALTMSKATYLVLDEADRMFDLGFEPQIRSIVGQIRPDRQTLLFS
Sbjct: 361 IVVATPGRLIDMIKMKALTMSKATYLVLDEADRMFDLGFEPQIRSIVGQIRPDRQTLLFS 420

Query: 421 ATMPRKIEKLAREILTDPVRVTVGEVGMANEDITQVVHVLPSDLEKLPWLLEKLPEMIDD 480
           ATMPRK+EKLAREILTDPVRVTVGEVGMANEDITQVVHVLPSDLEKLPWLLEKLPEMIDD
Sbjct: 421 ATMPRKVEKLAREILTDPVRVTVGEVGMANEDITQVVHVLPSDLEKLPWLLEKLPEMIDD 480

Query: 481 GDVLVFASKKATVDEIESQLQQKGFKVAALHGDKDQASRMETLQKFKSGVYHVLIATDVA 540
           GDVLVFASKKATVDEIESQL QKGFKVAALHGDKDQASRMETLQKFKSGVYH LIATDVA
Sbjct: 481 GDVLVFASKKATVDEIESQLLQKGFKVAALHGDKDQASRMETLQKFKSGVYHALIATDVA 540

Query: 541 ARGLDIKSIKSVVNFDIAKDMDMHVHRIGRTGRAGDKDGRAHTLITQKEARFAGDLVNSL 600
           ARGLDIKSIKSVVNFDIAKDMDMHVHRIGRTGRAGD+DG+AHTLITQKEARFAG+LVNSL
Sbjct: 541 ARGLDIKSIKSVVNFDIAKDMDMHVHRIGRTGRAGDRDGKAHTLITQKEARFAGELVNSL 600

Query: 601 IAAGQNVSVELMDLAMKDGRFRSKRDARKK----GGGGGGGKKGKGRGG-NGRGVRGVDF 660
           IAAGQNVS+ELMDLAMKDGRFRSKRDARKK    GGGGGGGKKGKGRGG NGRGVRGVDF
Sbjct: 601 IAAGQNVSMELMDLAMKDGRFRSKRDARKKGGVGGGGGGGGKKGKGRGGSNGRGVRGVDF 660

Query: 661 GLGIGYNPESSGSSPSTNNVQSRSAAVNSLRTGMIAQFKSNFVAASSAPPSQGSNNSYSV 720
           GLGIGYNPESSGS+PSTNNVQSRSAAVNSLRTGM+AQFKS+FVAASSAPPSQGSN+ YS+
Sbjct: 661 GLGIGYNPESSGSNPSTNNVQSRSAAVNSLRTGMMAQFKSSFVAASSAPPSQGSNSGYSM 720

Query: 721 PANKRPTLAGFVSGGSIGGGANSPAPPVPRANSYVPNAGEYSSQKNSESSGDRPRERRRP 778
           PANKRPTLAGFVSGGSIGGGAN P PP PRANSYVPNAGE+SSQKNSESSGDR +ERRRP
Sbjct: 721 PANKRPTLAGFVSGGSIGGGANRPPPPPPRANSYVPNAGEFSSQKNSESSGDRSKERRRP 780

BLAST of Lag0031060 vs. NCBI nr
Match: XP_022998395.1 (DEAD-box ATP-dependent RNA helicase 24 isoform X2 [Cucurbita maxima])

HSP 1 Score: 1426.4 bits (3691), Expect = 0.0e+00
Identity = 740/778 (95.12%), Postives = 761/778 (97.81%), Query Frame = 0

Query: 1   MSKRKFGFEGFGINRQTTYNFERSQAPQRLYVPPSSRGHGHDNYEDTDVDNIEYDDNETE 60
           MSKRKFGFEGFGINRQTTYNFERSQAPQRLYVPPSSRGHGHDNYEDTDVDNIEYDDNE E
Sbjct: 1   MSKRKFGFEGFGINRQTTYNFERSQAPQRLYVPPSSRGHGHDNYEDTDVDNIEYDDNEAE 60

Query: 61  EASGNGEAGGGAAGGGEEEEIDPLDAFMEGIHEEMRAPPPPKPKEKVDKYRDDEEDDPME 120
           EASGNG+   G AGGGEEEEIDPLDAFMEGIHEEM+APPPPKPKEKVDKYRDDEEDDPME
Sbjct: 61  EASGNGDNVAG-AGGGEEEEIDPLDAFMEGIHEEMKAPPPPKPKEKVDKYRDDEEDDPME 120

Query: 121 SFLRAKKDVGLTLAADALHAGYDSDEEVYAAAKAVDAGMVEYDSDDNMLILEKKKIEPIP 180
           SFL+AKKDVGLTLAADALHAGYDSDEEVYAAAKAVDA MVEYDSDDNMLI+EKKKIE IP
Sbjct: 121 SFLKAKKDVGLTLAADALHAGYDSDEEVYAAAKAVDAAMVEYDSDDNMLIVEKKKIESIP 180

Query: 181 ALDHSSIDYEPFNKDFYEEKASISGMSEEEVSEYRKSLAIRVSGFDVPRPIKTFEDCGFS 240
           AL+HSSIDYEPFNKDFYEEKASISGMSEEEVSEYRK+LAIRVSGFDVPRPIKTFED GFS
Sbjct: 181 ALEHSSIDYEPFNKDFYEEKASISGMSEEEVSEYRKNLAIRVSGFDVPRPIKTFEDSGFS 240

Query: 241 PQLMNAIKKQGYEKPTSIQCQAMPIVLSGRDIIGIAKTGSGKTAAFVLPMIVHIMDQPEL 300
            QLMNAIKKQGYEKPTSIQCQAMPIVLSGRDIIGIAKTGSGKTA+FVLPMIVHIMDQPEL
Sbjct: 241 LQLMNAIKKQGYEKPTSIQCQAMPIVLSGRDIIGIAKTGSGKTASFVLPMIVHIMDQPEL 300

Query: 301 EKEEGPIGVICAPTRELAHQIYLECKKFSKAHGLRVSAVYGGMSKFDQLKELKAGCEIVV 360
           EKEEGPIGVICAPTRELAHQIYLECKKFSK HGLRVSAVYGGMSKFDQ+KELKAGCEIVV
Sbjct: 301 EKEEGPIGVICAPTRELAHQIYLECKKFSKVHGLRVSAVYGGMSKFDQIKELKAGCEIVV 360

Query: 361 ATPGRLIDMIKMKALTMSKATYLVLDEADRMFDLGFEPQIRSIVGQIRPDRQTLLFSATM 420
           ATPGRLIDMIKMKALTMSKATYLVLDEADRMFDLGFEPQIRSIVGQIRPDRQTLLFSATM
Sbjct: 361 ATPGRLIDMIKMKALTMSKATYLVLDEADRMFDLGFEPQIRSIVGQIRPDRQTLLFSATM 420

Query: 421 PRKIEKLAREILTDPVRVTVGEVGMANEDITQVVHVLPSDLEKLPWLLEKLPEMIDDGDV 480
           PRK+EKLAREILTDPVRVTVGEVGMANEDITQVVHVLPSDLEKLPWLLEKLPEMIDDGDV
Sbjct: 421 PRKVEKLAREILTDPVRVTVGEVGMANEDITQVVHVLPSDLEKLPWLLEKLPEMIDDGDV 480

Query: 481 LVFASKKATVDEIESQLQQKGFKVAALHGDKDQASRMETLQKFKSGVYHVLIATDVAARG 540
           LVFASKKATVDEIESQL QKGFKVAALHGDKDQASRMETLQKFKSGVYH LIATDVAARG
Sbjct: 481 LVFASKKATVDEIESQLLQKGFKVAALHGDKDQASRMETLQKFKSGVYHALIATDVAARG 540

Query: 541 LDIKSIKSVVNFDIAKDMDMHVHRIGRTGRAGDKDGRAHTLITQKEARFAGDLVNSLIAA 600
           LDIKSIKSVVNFDIAKDMDMHVHRIGRTGRAGD+DG+AHTLITQKEARFAG+LVNSLIAA
Sbjct: 541 LDIKSIKSVVNFDIAKDMDMHVHRIGRTGRAGDRDGKAHTLITQKEARFAGELVNSLIAA 600

Query: 601 GQNVSVELMDLAMKDGRFRSKRDARKKGGGGGGGKKGKGR-GGNGRGVRGVDFGLGIGYN 660
           GQNVS+ELMDLAMKDGRFRSKRDARKKGGGGGGGKKGKGR GGNGRGVRGVDFGLGIGYN
Sbjct: 601 GQNVSMELMDLAMKDGRFRSKRDARKKGGGGGGGKKGKGRGGGNGRGVRGVDFGLGIGYN 660

Query: 661 PESSGSSPSTNNVQSRSAAVNSLRTGMIAQFKSNFVAASSAPPSQGSNNSYSVPANKRPT 720
           PESSGS+PSTNNVQSRSAAVNSLRTGM+AQFK++FVAASSAPPSQGSN+SYS+PANKRPT
Sbjct: 661 PESSGSNPSTNNVQSRSAAVNSLRTGMMAQFKNSFVAASSAPPSQGSNSSYSMPANKRPT 720

Query: 721 LAGFVSGGSIGGGANSPAPPVPRANSYVPNAGEYSSQKNSESSGDRPRERRRPSGWDR 778
           LAGFVSGGSIGGGAN P PP PRANSYVPNAGE+SSQKNSESSGDR +ERRRPSGWDR
Sbjct: 721 LAGFVSGGSIGGGANRPPPPAPRANSYVPNAGEFSSQKNSESSGDRSKERRRPSGWDR 777

BLAST of Lag0031060 vs. ExPASy Swiss-Prot
Match: O22907 (DEAD-box ATP-dependent RNA helicase 24 OS=Arabidopsis thaliana OX=3702 GN=RH24 PE=1 SV=2)

HSP 1 Score: 1122.5 bits (2902), Expect = 0.0e+00
Identity = 595/780 (76.28%), Postives = 682/780 (87.44%), Query Frame = 0

Query: 1   MSKRKFGFEGFGINRQTTYNFERSQAPQRLYVPPSSRGHGHDNYEDTDVDNIEYDDNETE 60
           MS RKFG EGFGINRQT+Y+FERSQAPQRLYVPPSSR  G DN ED D+DNI+Y +NE  
Sbjct: 1   MSNRKFGMEGFGINRQTSYSFERSQAPQRLYVPPSSR--GGDNSEDADLDNIDYMENEEA 60

Query: 61  EASGNGEAGGGAAGGGEEEEIDPLDAFMEGIHEEMRAPPPPKPKEKVDKYRDDEEDDPME 120
           E     E G  AA GGE +EIDPLDAFMEGIH+EM++ PPPKPKEK+++Y+DD +DDP+E
Sbjct: 61  EEDIE-EGGSAAASGGEVDEIDPLDAFMEGIHQEMKSAPPPKPKEKLERYKDD-DDDPVE 120

Query: 121 SFLRAKKDVGLTLAADALHAGYDSDEEVYAAAKAVDAGMVEYDSDDNMLILEKKKIEPIP 180
           S+L+AKKD+GLTLAADAL+AGY+SDEEVYAAAKAVDAGM++YDSDDN ++++K+KIEPI 
Sbjct: 121 SYLKAKKDLGLTLAADALNAGYNSDEEVYAAAKAVDAGMLDYDSDDNPIVVDKRKIEPIT 180

Query: 181 ALDHSSIDYEPFNKDFYEEKASISGMSEEEVSEYRKSLAIRVSGFDVPRPIKTFEDCGFS 240
           ALDHSSIDYEP NKDFYEE  SISGM+E+E ++YR+ L IRVSGFDV RP+KTFEDCGFS
Sbjct: 181 ALDHSSIDYEPINKDFYEELESISGMTEQETTDYRQRLGIRVSGFDVHRPVKTFEDCGFS 240

Query: 241 PQLMNAIKKQGYEKPTSIQCQAMPIVLSGRDIIGIAKTGSGKTAAFVLPMIVHIMDQPEL 300
            Q+M+AIKKQ YEKPT+IQCQA+PIVLSGRD+IGIAKTGSGKTAAFVLPMIVHIMDQPEL
Sbjct: 241 SQIMSAIKKQAYEKPTAIQCQALPIVLSGRDVIGIAKTGSGKTAAFVLPMIVHIMDQPEL 300

Query: 301 EKEEGPIGVICAPTRELAHQIYLECKKFSKAHGLRVSAVYGGMSKFDQLKELKAGCEIVV 360
           +++EGPIGVICAPTRELAHQI+LE KKFSKA+GLRVSAVYGGMSK +Q KELKAGCEIVV
Sbjct: 301 QRDEGPIGVICAPTRELAHQIFLEAKKFSKAYGLRVSAVYGGMSKHEQFKELKAGCEIVV 360

Query: 361 ATPGRLIDMIKMKALTMSKATYLVLDEADRMFDLGFEPQIRSIVGQIRPDRQTLLFSATM 420
           ATPGRLIDM+KMKALTM +A+YLVLDEADRMFDLGFEPQ+RSIVGQIRPDRQTLLFSATM
Sbjct: 361 ATPGRLIDMLKMKALTMMRASYLVLDEADRMFDLGFEPQVRSIVGQIRPDRQTLLFSATM 420

Query: 421 PRKIEKLAREILTDPVRVTVGEVGMANEDITQVVHVLPSDLEKLPWLLEKLPEMIDDGDV 480
           P K+EKLAREIL+DP+RVTVGEVGMANEDITQVV+V+PSD EKLPWLLEKLP MID+GDV
Sbjct: 421 PWKVEKLAREILSDPIRVTVGEVGMANEDITQVVNVIPSDAEKLPWLLEKLPGMIDEGDV 480

Query: 481 LVFASKKATVDEIESQLQQKGFKVAALHGDKDQASRMETLQKFKSGVYHVLIATDVAARG 540
           LVFASKKATVDEIE+QL    FKVAALHGDKDQASRMETLQKFKSGV+HVLIATDVAARG
Sbjct: 481 LVFASKKATVDEIEAQLTLNSFKVAALHGDKDQASRMETLQKFKSGVHHVLIATDVAARG 540

Query: 541 LDIKSIKSVVNFDIAKDMDMHVHRIGRTGRAGDKDGRAHTLITQKEARFAGDLVNSLIAA 600
           LDIKS+K+VVN+DIAKDMDMHVHRIGRTGRAGD+DG A+TL+TQ+EARFAG+LVNSL+AA
Sbjct: 541 LDIKSLKTVVNYDIAKDMDMHVHRIGRTGRAGDRDGVAYTLVTQREARFAGELVNSLVAA 600

Query: 601 GQNVSVELMDLAMKDGRFRSKRDARKKGGGGGGGKKGKGRGGNGRGVRGVDFGLGIGYNP 660
           GQNV  EL DLAMKDGRF+SKRD RK      GGKKG+G GG  +GVRGVDFGLGIG++ 
Sbjct: 601 GQNVPPELTDLAMKDGRFKSKRDGRK------GGKKGRGGGGGNKGVRGVDFGLGIGFSS 660

Query: 661 ESSGSSPSTNNVQSRSAAVNSLRTGMIAQFKSNFVAASSAPPSQGSNNSYSVPANKRPTL 720
           ESS  +PS+    SRS A+NS+RTG++AQFK++FVAA+   PS   N +Y    NKRP+L
Sbjct: 661 ESS-RTPSSKAAPSRSGAINSVRTGVMAQFKNSFVAAT---PSNPQNQAY---PNKRPSL 720

Query: 721 AGFVSGGSIGGG---ANSPAPPV-PRANSYVPNAGEYSSQKNSESSGDRPRERRRPSGWD 777
            GFVSGG+IGG      S APPV P  N+   N    SSQ +S+SS +RPRER+R SGWD
Sbjct: 721 MGFVSGGTIGGDMGRTQSQAPPVAPTQNASSHN----SSQNHSQSSENRPRERKRRSGWD 759

BLAST of Lag0031060 vs. ExPASy Swiss-Prot
Match: Q10MH8 (DEAD-box ATP-dependent RNA helicase 24 OS=Oryza sativa subsp. japonica OX=39947 GN=Os03g0308500 PE=2 SV=1)

HSP 1 Score: 1065.1 bits (2753), Expect = 3.8e-310
Identity = 582/791 (73.58%), Postives = 654/791 (82.68%), Query Frame = 0

Query: 1   MSKR-KFGFEGFGINRQTTYNFERSQAPQRLYVPPSSRGHGHDNYEDTDVDNIEYDDNET 60
           MSKR K G  GF I R T+Y+FERSQ PQRLYVP           +D D+D+I + D+  
Sbjct: 1   MSKRPKLG--GFSIPRPTSYSFERSQPPQRLYVPA----------DDPDLDDIAFSDDAA 60

Query: 61  EEASGNGEAGGGAAGGGEEEEIDPLDAFMEGIHEEMRAPPPPKPKEKVDKYRDDEEDDPM 120
             +      GGGAA  G+EEEIDPLDAFM  I EE+RAPPP    E + +   D+EDDP+
Sbjct: 61  APSDAPPAGGGGAA--GDEEEIDPLDAFMAEIQEEIRAPPPAPKPEALRRADSDDEDDPV 120

Query: 121 ESFLRAKKDVGLTLAADALHAGYDSDEEVYAAAKAVDAGMVEYDSDDNMLILEKKKIEPI 180
           ESFLRAKKD GL LAADA+HAGYDSDEEVYAAAKAVDAGM+EYDSDDN ++++KKKIEPI
Sbjct: 121 ESFLRAKKDSGLALAADAMHAGYDSDEEVYAAAKAVDAGMMEYDSDDNPIVVDKKKIEPI 180

Query: 181 PALDHSSIDYEPFNKDFYEEKASISGMSEEEVSEYRKSLAIRVSGFDVPRPIKTFEDCGF 240
           P LDHS+I+YEPFNKDFYEEK S+SGMSE+EV++Y KSLAIRVSGFDVPRPIK+F DCGF
Sbjct: 181 PPLDHSTIEYEPFNKDFYEEKPSVSGMSEQEVADYMKSLAIRVSGFDVPRPIKSFADCGF 240

Query: 241 SPQLMNAIKKQGYEKPTSIQCQAMPIVLSGRDIIGIAKTGSGKTAAFVLPMIVHIMDQPE 300
             QLMNAI KQGYEKPT+IQCQA+PIVLSGRDIIGIAKTGSGKTAAFVLPMIVHIMDQPE
Sbjct: 241 PVQLMNAIAKQGYEKPTTIQCQALPIVLSGRDIIGIAKTGSGKTAAFVLPMIVHIMDQPE 300

Query: 301 LEKEEGPIGVICAPTRELAHQIYLECKKFSKAHGLRVSAVYGGMSKFDQLKELKAGCEIV 360
           LEKEEGPIGV+CAPTRELAHQIYLE KKF+K + LRV+AVYGG+SKFDQ KELKAGCEIV
Sbjct: 301 LEKEEGPIGVVCAPTRELAHQIYLEAKKFAKPYNLRVAAVYGGVSKFDQFKELKAGCEIV 360

Query: 361 VATPGRLIDMIKMKALTMSKATYLVLDEADRMFDLGFEPQIRSIVGQIRPDRQTLLFSAT 420
           +ATPGRLID++KMKAL M +ATYLVLDEADRMFDLGFEPQIRSIVGQIRPDRQTLLFSAT
Sbjct: 361 IATPGRLIDLLKMKALKMFRATYLVLDEADRMFDLGFEPQIRSIVGQIRPDRQTLLFSAT 420

Query: 421 MPRKIEKLAREILTDPVRVTVGEVGMANEDITQVVHVLPSDLEKLPWLLEKLPEMIDDGD 480
           MP K+E+LAREILTDP+RVTVG+VG ANEDI QVV+VLPSD EK+PWLLEKLP MIDDGD
Sbjct: 421 MPYKVERLAREILTDPIRVTVGQVGSANEDIKQVVNVLPSDAEKMPWLLEKLPGMIDDGD 480

Query: 481 VLVFASKKATVDEIESQLQQKGFKVAALHGDKDQASRMETLQKFKSGVYHVLIATDVAAR 540
           VLVFA+KKA VDEIESQL Q+GF++AALHGDKDQASRMETLQKFKSGVYHVL+ATDVAAR
Sbjct: 481 VLVFAAKKARVDEIESQLNQRGFRIAALHGDKDQASRMETLQKFKSGVYHVLVATDVAAR 540

Query: 541 GLDIKSIKSVVNFDIAKDMDMHVHRIGRTGRAGDKDGRAHTLITQKEARFAGDLVNSLIA 600
           GLDIKSIK+VVNFDIAK+MDMH+HRIGRTGRAGDKDG A+TLITQKE RFAG+LV+ LIA
Sbjct: 541 GLDIKSIKTVVNFDIAKEMDMHIHRIGRTGRAGDKDGTAYTLITQKEVRFAGELVHCLIA 600

Query: 601 AGQNVSVELMDLAMKDGRFRSKRDARKKG-------GGGGGGKKGKGRGGNGRGVRGVDF 660
           AGQ+V  ELMDLAMKDGRFR+ RD+RK G       GGGGGG  G G  G GRGVRGVDF
Sbjct: 601 AGQDVPNELMDLAMKDGRFRANRDSRKGGKKSGKGKGGGGGGGGGSGARGRGRGVRGVDF 660

Query: 661 GLGIGYNPESSGSSPSTNNVQSRSAAVNSLRTGMIAQFKSNFVAASSAPPSQGSNNSYSV 720
           GLGIGYN E SGS P+      RSAA+NSL+TGM+  FKS+FV+ASS+     S  S   
Sbjct: 661 GLGIGYNAE-SGSVPA-----PRSAALNSLKTGMMQNFKSSFVSASSSNTPSNSAPSRGA 720

Query: 721 PAN-KRPTLAGFVSGGSIGGGANS-----PAPPVPRANSYVPNAGEYSSQKNSESSGDRP 778
           P++  RP L GFVSGG+IGG AN      PAP    A+    N  E ++  N ESS DR 
Sbjct: 721 PSSFVRPALRGFVSGGTIGGDANQARAVLPAPSFVPASRPAENTVE-NANPNPESSRDRT 770

BLAST of Lag0031060 vs. ExPASy Swiss-Prot
Match: Q810A7 (ATP-dependent RNA helicase DDX42 OS=Mus musculus OX=10090 GN=Ddx42 PE=1 SV=3)

HSP 1 Score: 529.3 bits (1362), Expect = 7.6e-149
Identity = 342/824 (41.50%), Postives = 472/824 (57.28%), Query Frame = 0

Query: 2   SKRKFGFEGFGIN-------------------RQTTYNFERSQAPQ--RLYVPPSSRGHG 61
           +KR FGF GF I+                     ++  F +S  PQ    Y   S R + 
Sbjct: 10  TKRGFGFGGFAISAGKKEEAKLPQQSHSAFGAASSSSGFGKSAPPQLPSFYKIGSKRANF 69

Query: 62  HDN---YEDTDVDNIEYDDNETEEASGNGEAGGGAAGGGEEEEIDPLDAFMEGIHEE--- 121
            +    +ED + D+   D       +        +     + + DPL+AFM  + ++   
Sbjct: 70  DEENAYFEDEEEDSSNVDLPYIPAENSPTRQQFHSKPADSDSDDDPLEAFMAEVEDQAAR 129

Query: 122 --MRAPPPPKPKEKVDKYRDD-EEDDPMESFLRAKKDVGLTLAADALHAGYDSDEEVYAA 181
              R     K ++ V   RDD EE+D  E++ R                 Y ++      
Sbjct: 130 DMKRLEEKDKERKNVKGIRDDIEEEDDQEAYFR-----------------YMAENPTAGV 189

Query: 182 AKAVDAGMVEYDSDDNMLILEKKKIEPIPALDHSSIDYEPFNKDFYEEKASISGMSEEEV 241
            +  +   +EYDSD N +   KK I+P+P +DHS IDY PF K+FY E   I+ ++ +++
Sbjct: 190 VQEEEEDNLEYDSDGNPIAPSKKIIDPLPPIDHSEIDYPPFEKNFYNEHEEITNLTPQQL 249

Query: 242 SEYRKSLAIRVSGFDVPRPIKTFEDCGFSPQLMNAIKKQGYEKPTSIQCQAMPIVLSGRD 301
            + R  L +RVSG   PRP  +F   GF  QLM+ I+K  Y +PT IQCQ +P+ LSGRD
Sbjct: 250 IDLRHKLNLRVSGAAPPRPGSSFAHFGFDEQLMHQIRKSEYTQPTPIQCQGVPVALSGRD 309

Query: 302 IIGIAKTGSGKTAAFVLPMIVHIMDQPELEKEEGPIGVICAPTRELAHQIYLECKKFSKA 361
           +IGIAKTGSGKTAAF+ PM++HIMDQ ELE  +GPI VI  PTREL  QI+ ECK+F KA
Sbjct: 310 MIGIAKTGSGKTAAFIWPMLIHIMDQKELEPGDGPIAVIVCPTRELCQQIHAECKRFGKA 369

Query: 362 HGLRVSAVYGGMSKFDQLKELKAGCEIVVATPGRLIDMIKMKALTMSKATYLVLDEADRM 421
           + LR  AVYGG S ++Q K L+ G EIVV TPGRLID +K KA  + + +YLV DEADRM
Sbjct: 370 YNLRSVAVYGGGSMWEQAKALQEGAEIVVCTPGRLIDHVKKKATNLQRVSYLVFDEADRM 429

Query: 422 FDLGFEPQIRSIVGQIRPDRQTLLFSATMPRKIEKLAREILTDPVRVTVGEVGMANEDIT 481
           FD+GFE Q+RSI   +RPDRQTLLFSAT  +KIEKLAR+IL DP+RV  G++G ANED+T
Sbjct: 430 FDMGFEYQVRSIASHVRPDRQTLLFSATFRKKIEKLARDILIDPIRVVQGDIGEANEDVT 489

Query: 482 QVVHVLPSDLEKLPWLLEKLPEMIDDGDVLVFASKKATVDEIESQLQQKGFKVAALHGDK 541
           Q+V +L S   K  WL  +L E    G VL+F +KKA  +E+ S L+Q+G  +  LHGD 
Sbjct: 490 QIVEILHSGPSKWNWLTRRLVEFTSSGSVLLFVTKKANAEELASNLKQEGHNLGLLHGDM 549

Query: 542 DQASRMETLQKFKSGVYHVLIATDVAARGLDIKSIKSVVNFDIAKDMDMHVHRIGRTGRA 601
           DQ+ R + +  FK     VL+ATDVAARGLDI SIK+V+N+D+A+D+D H HRIGRTGRA
Sbjct: 550 DQSERNKVISDFKKKDIPVLVATDVAARGLDIPSIKTVINYDVARDIDTHTHRIGRTGRA 609

Query: 602 GDKDGRAHTLITQKEARFAGDLVNSLIAAGQNVSVELMDLAMKDGRFRSKRDARKKG--- 661
           G+K G A+TL+T K++ FAGDLV +L  A Q+VS EL+DLAM++  FR  R    KG   
Sbjct: 610 GEK-GVAYTLLTPKDSNFAGDLVRNLEGANQHVSKELLDLAMQNAWFRKSRFKGGKGKKL 669

Query: 662 --GGGGGGKKGKGRGGNGRGVRGVDFGLGIGYNPESSGSSPSTNNVQSRSAAVNSLRTGM 721
             GGGG G + +   G+    RG +  +   Y        PST  +  R   + +++   
Sbjct: 670 NIGGGGLGYRERPGLGSENSDRGNNNNVMSNY----EAYKPSTGAMGDR---LTAMKAAF 729

Query: 722 IAQFKSNFVAASSAPPSQGSNNSYSVPANKRPTLAGFVSGGSIGG--------GANSPAP 769
            +Q+KS+FVAAS +    G++++ +         +G+ S GS+          G NSP  
Sbjct: 730 QSQYKSHFVAASLSNQKAGTSSAGA---------SGWTSAGSLNSVPTNSAQQGHNSPDN 789

BLAST of Lag0031060 vs. ExPASy Swiss-Prot
Match: Q86XP3 (ATP-dependent RNA helicase DDX42 OS=Homo sapiens OX=9606 GN=DDX42 PE=1 SV=1)

HSP 1 Score: 528.9 bits (1361), Expect = 9.9e-149
Identity = 336/793 (42.37%), Postives = 457/793 (57.63%), Query Frame = 0

Query: 2   SKRKFGFEGFGIN-------------------RQTTYNFERSQAPQ--RLYVPPSSRGHG 61
           +KR FGF GF I+                     ++  F +S  PQ    Y   S R + 
Sbjct: 10  TKRGFGFGGFAISAGKKEEPKLPQQSHSAFGATSSSSGFGKSAPPQLPSFYKIGSKRANF 69

Query: 62  HDN---YEDTDVDNIEYDDNETEEASGNGEAGGGAAGGGEEEEIDPLDAFMEGIHEE--- 121
            +    +ED + D+   D       +        +     + + DPL+AFM  + ++   
Sbjct: 70  DEENAYFEDEEEDSSNVDLPYIPAENSPTRQQFHSKPVDSDSDDDPLEAFMAEVEDQAAR 129

Query: 122 --MRAPPPPKPKEKVDKYRDD-EEDDPMESFLRAKKDVGLTLAADALHAGYDSDEEVYAA 181
              R     K ++ V   RDD EE+D  E++ R                 Y ++      
Sbjct: 130 DMKRLEEKDKERKNVKGIRDDIEEEDDQEAYFR-----------------YMAENPTAGV 189

Query: 182 AKAVDAGMVEYDSDDNMLILEKKKIEPIPALDHSSIDYEPFNKDFYEEKASISGMSEEEV 241
            +  +   +EYDSD N +   KK I+P+P +DHS IDY PF K+FY E   I+ ++ +++
Sbjct: 190 VQEEEEDNLEYDSDGNPIAPTKKIIDPLPPIDHSEIDYPPFEKNFYNEHEEITNLTPQQL 249

Query: 242 SEYRKSLAIRVSGFDVPRPIKTFEDCGFSPQLMNAIKKQGYEKPTSIQCQAMPIVLSGRD 301
            + R  L +RVSG   PRP  +F   GF  QLM+ I+K  Y +PT IQCQ +P+ LSGRD
Sbjct: 250 IDLRHKLNLRVSGAAPPRPGSSFAHFGFDEQLMHQIRKSEYTQPTPIQCQGVPVALSGRD 309

Query: 302 IIGIAKTGSGKTAAFVLPMIVHIMDQPELEKEEGPIGVICAPTRELAHQIYLECKKFSKA 361
           +IGIAKTGSGKTAAF+ PM++HIMDQ ELE  +GPI VI  PTREL  QI+ ECK+F KA
Sbjct: 310 MIGIAKTGSGKTAAFIWPMLIHIMDQKELEPGDGPIAVIVCPTRELCQQIHAECKRFGKA 369

Query: 362 HGLRVSAVYGGMSKFDQLKELKAGCEIVVATPGRLIDMIKMKALTMSKATYLVLDEADRM 421
           + LR  AVYGG S ++Q K L+ G EIVV TPGRLID +K KA  + + +YLV DEADRM
Sbjct: 370 YNLRSVAVYGGGSMWEQAKALQEGAEIVVCTPGRLIDHVKKKATNLQRVSYLVFDEADRM 429

Query: 422 FDLGFEPQIRSIVGQIRPDRQTLLFSATMPRKIEKLAREILTDPVRVTVGEVGMANEDIT 481
           FD+GFE Q+RSI   +RPDRQTLLFSAT  +KIEKLAR+IL DP+RV  G++G ANED+T
Sbjct: 430 FDMGFEYQVRSIASHVRPDRQTLLFSATFRKKIEKLARDILIDPIRVVQGDIGEANEDVT 489

Query: 482 QVVHVLPSDLEKLPWLLEKLPEMIDDGDVLVFASKKATVDEIESQLQQKGFKVAALHGDK 541
           Q+V +L S   K  WL  +L E    G VL+F +KKA  +E+ + L+Q+G  +  LHGD 
Sbjct: 490 QIVEILHSGPSKWNWLTRRLVEFTSSGSVLLFVTKKANAEELANNLKQEGHNLGLLHGDM 549

Query: 542 DQASRMETLQKFKSGVYHVLIATDVAARGLDIKSIKSVVNFDIAKDMDMHVHRIGRTGRA 601
           DQ+ R + +  FK     VL+ATDVAARGLDI SIK+V+N+D+A+D+D H HRIGRTGRA
Sbjct: 550 DQSERNKVISDFKKKDIPVLVATDVAARGLDIPSIKTVINYDVARDIDTHTHRIGRTGRA 609

Query: 602 GDKDGRAHTLITQKEARFAGDLVNSLIAAGQNVSVELMDLAMKDGRFRSKRDARKKG--- 661
           G+K G A+TL+T K++ FAGDLV +L  A Q+VS EL+DLAM++  FR  R    KG   
Sbjct: 610 GEK-GVAYTLLTPKDSNFAGDLVRNLEGANQHVSKELLDLAMQNAWFRKSRFKGGKGKKL 669

Query: 662 --GGGGGGKKGKGRGGNGRGVRGVDFGLGIGYNPESSGSSPSTNNVQSRSAAVNSLRTGM 721
             GGGG G + +     G G   +D G             PST  +  R   + +++   
Sbjct: 670 NIGGGGLGYRER----PGLGSENMDRG-NNNVMSNYEAYKPSTGAMGDR---LTAMKAAF 729

Query: 722 IAQFKSNFVAASSAPPSQGSNNSYSVPANKRPTLAGFVSGGSIGG--------GANSPAP 752
            +Q+KS+FVAAS +    GS+ + +         +G+ S GS+          G NSP  
Sbjct: 730 QSQYKSHFVAASLSNQKAGSSAAGA---------SGWTSAGSLNSVPTNSAQQGHNSPDS 767

BLAST of Lag0031060 vs. ExPASy Swiss-Prot
Match: Q54IV3 (Probable ATP-dependent RNA helicase ddx42 OS=Dictyostelium discoideum OX=44689 GN=ddx42 PE=3 SV=1)

HSP 1 Score: 526.9 bits (1356), Expect = 3.8e-148
Identity = 305/651 (46.85%), Postives = 416/651 (63.90%), Query Frame = 0

Query: 32  VPPSSR----GHGHDNYEDTDVDNIEYDDNETEEASGNGEAGGGAAGGGEEEEIDPLDAF 91
           VPP S          N+E+   +N   ++N  +E+    +         E++EIDPLDAF
Sbjct: 104 VPPKSSMTTLNKSPPNFENASSNNNNNNNNNNQESDSKNQ------NEDEDDEIDPLDAF 163

Query: 92  MEGIHEE------MRAPPPPKPKEKVDKYRDD-EEDDPMESFLRAKKDVGLTLAADALHA 151
           ME ++ +             + ++ +   RDD + +D  E F + ++     LA  +   
Sbjct: 164 MENVNAQAAIDNSKSIEKGQQQQQSLKSKRDDIDNEDDEEIFYKLRQK---QLANKSSKQ 223

Query: 152 GYDSDEEVYAAAKAVDAGMVEYDSDDNMLILEKKKIEPIPALDHSSIDYEPFNKDFYEEK 211
             D D + Y++    D    + +S  N     K+ IEP+P +DHS  +Y  FNK FYEE 
Sbjct: 224 QQDDDVD-YSSLDDDDGYFDDEESLKNGQSKGKRIIEPLPPIDHSKEEYIEFNKIFYEEH 283

Query: 212 ASISGMSEEEVSEYRKSLAIRVSGFDVPRPIKTFEDCGFSPQLMNAIKKQGYEKPTSIQC 271
             I+ ++EE+V E RK+L IR++G D+  P+ +F   GF   L+ AI KQ  E PT IQ 
Sbjct: 284 PDIANLTEEQVFEIRKNLDIRMTGTDLINPVTSFGHYGFDDILLQAIAKQSIETPTPIQK 343

Query: 272 QAMPIVLSGRDIIGIAKTGSGKTAAFVLPMIVHIMDQPELEKEEGPIGVICAPTRELAHQ 331
           QA+PI LSGRD+I IAKTGSGKTA F+ P I HIMDQP LEK +GPI +  APTRELAHQ
Sbjct: 344 QAIPIALSGRDLIAIAKTGSGKTATFIWPSISHIMDQPYLEKGDGPIALFLAPTRELAHQ 403

Query: 332 IYLECKKFSKAHGLRVSAVYGGMSKFDQLKELKAGCEIVVATPGRLIDMIKMKALTMSKA 391
           IYLE  K+SK   L+ + +YGG+SK  Q KELKAGCEI+VATPGRLIDMIK+KA  +++ 
Sbjct: 404 IYLETLKYSKYFKLKTTVLYGGVSKQQQCKELKAGCEIIVATPGRLIDMIKLKATKLNRV 463

Query: 392 TYLVLDEADRMFDLGFEPQIRSIVGQIRPDRQTLLFSATMPRKIEKLAREILTDPVRVTV 451
           +YLVLDEAD+MFD GF PQ+ SIV  +RPDRQTLLFSAT    +E+ AR IL+DP+++++
Sbjct: 464 SYLVLDEADKMFDFGFGPQVLSIVNHVRPDRQTLLFSATFKPNVEEFARTILSDPIKISI 523

Query: 452 GEVGMANEDITQVVHVLPSDLEKLPWLLEKLPEMIDDGDVLVFASKKATVDEIESQLQQK 511
           G +G AN DITQ+V VL SD +K  WL  +L  ++  G VL+F S K  V+++ S L + 
Sbjct: 524 GMIGSANSDITQIVQVLKSDSDKWNWLTNQLALLLSQGSVLIFVSTKVAVEQLSSNLTKF 583

Query: 512 GFKVAALHGDKDQASRMETLQKFKSGVYHVLIATDVAARGLDIKSIKSVVNFDIAKDMDM 571
           GF+   LHGDK+Q  R +T+Q FK G  ++LIATDVAARGLDI  IK+VVN+D ++D++ 
Sbjct: 584 GFQTCTLHGDKNQIERSQTIQTFKEGKINILIATDVAARGLDIPLIKNVVNYDTSRDIES 643

Query: 572 HVHRIGRTGRAGDKDGRAHTLITQKEARFAGDLVNSLIAAGQNVSVELMDLAMKDGRFRS 631
           H HRIGRTGRAG+  G A+TLIT K+  F+ DL+ +L +A Q V  EL+D+AM +  F+ 
Sbjct: 644 HTHRIGRTGRAGN-TGVAYTLITPKDIHFSVDLIKNLESASQFVPPELIDVAMNNPHFK- 703

Query: 632 KRDARKKGGGGGGGKKGKGRGGNGRGVRGVDFGLGIGYNPESSGSSPSTNN 672
               R++GGGGGG  +G+GRGG G G R    G G+ +N     S   + N
Sbjct: 704 ----RERGGGGGGSNRGRGRGGGGVGYRRNSRGGGVAFNSNRDSSRSDSQN 738

BLAST of Lag0031060 vs. ExPASy TrEMBL
Match: A0A1S3BY83 (DEAD-box ATP-dependent RNA helicase 24 OS=Cucumis melo OX=3656 GN=LOC103494724 PE=3 SV=1)

HSP 1 Score: 1436.8 bits (3718), Expect = 0.0e+00
Identity = 743/777 (95.62%), Postives = 760/777 (97.81%), Query Frame = 0

Query: 1   MSKRKFGFEGFGINRQTTYNFERSQAPQRLYVPPSSRGHGHDNYEDTDVDNIEYDDNETE 60
           MSKRKFGFEGFGINRQTTYNFERSQAPQRLYVPPSSRGHGHDNYEDTDVDNIEYDDN+ E
Sbjct: 1   MSKRKFGFEGFGINRQTTYNFERSQAPQRLYVPPSSRGHGHDNYEDTDVDNIEYDDNDGE 60

Query: 61  EASGNGEAGGGAAGGGEEEEIDPLDAFMEGIHEEMRAPPPPKPKEKVDKYRDDEEDDPME 120
           EASGN + G GAA G EEEEIDPLDAFMEGIHEEM+APPPPKPKEKVDKYRDDEEDDPME
Sbjct: 61  EASGNDDGGAGAA-GAEEEEIDPLDAFMEGIHEEMKAPPPPKPKEKVDKYRDDEEDDPME 120

Query: 121 SFLRAKKDVGLTLAADALHAGYDSDEEVYAAAKAVDAGMVEYDSDDNMLILEKKKIEPIP 180
           SFLRAKKDVGLTLAADALHAGYDSDEEVYAAAKAVDAGMVEYDSDDNMLI+EKKKIEPIP
Sbjct: 121 SFLRAKKDVGLTLAADALHAGYDSDEEVYAAAKAVDAGMVEYDSDDNMLIVEKKKIEPIP 180

Query: 181 ALDHSSIDYEPFNKDFYEEKASISGMSEEEVSEYRKSLAIRVSGFDVPRPIKTFEDCGFS 240
           ALDHSSIDYEPFNKDFYEEKASISGMSEEEVSEYRKSLAIRVSGFDVPRPIKTFEDCGFS
Sbjct: 181 ALDHSSIDYEPFNKDFYEEKASISGMSEEEVSEYRKSLAIRVSGFDVPRPIKTFEDCGFS 240

Query: 241 PQLMNAIKKQGYEKPTSIQCQAMPIVLSGRDIIGIAKTGSGKTAAFVLPMIVHIMDQPEL 300
           PQLMNAIKKQGYEKPTSIQCQAMPIVLSG DIIGIAKTGSGKTAAFVLPMIVHIMDQPEL
Sbjct: 241 PQLMNAIKKQGYEKPTSIQCQAMPIVLSGGDIIGIAKTGSGKTAAFVLPMIVHIMDQPEL 300

Query: 301 EKEEGPIGVICAPTRELAHQIYLECKKFSKAHGLRVSAVYGGMSKFDQLKELKAGCEIVV 360
           EKEEGPIGVICAPTRELAHQIYLECKKFSKAHGLRVSAVYGGMSKFDQLKELKAGCEIVV
Sbjct: 301 EKEEGPIGVICAPTRELAHQIYLECKKFSKAHGLRVSAVYGGMSKFDQLKELKAGCEIVV 360

Query: 361 ATPGRLIDMIKMKALTMSKATYLVLDEADRMFDLGFEPQIRSIVGQIRPDRQTLLFSATM 420
           ATPGRLIDMIK+KALTMSKATYLVLDEADRMFDLGFEPQIRSIVGQIRPDRQTLLFSATM
Sbjct: 361 ATPGRLIDMIKLKALTMSKATYLVLDEADRMFDLGFEPQIRSIVGQIRPDRQTLLFSATM 420

Query: 421 PRKIEKLAREILTDPVRVTVGEVGMANEDITQVVHVLPSDLEKLPWLLEKLPEMIDDGDV 480
           PRK+EKLAREILTDPVRVTVGEVGMANEDITQVVHVLPSDLEKLPWLLEKLPEMIDDGDV
Sbjct: 421 PRKVEKLAREILTDPVRVTVGEVGMANEDITQVVHVLPSDLEKLPWLLEKLPEMIDDGDV 480

Query: 481 LVFASKKATVDEIESQLQQKGFKVAALHGDKDQASRMETLQKFKSGVYHVLIATDVAARG 540
           LVFASKKATVDE+ESQL QKGFKVAALHGDKDQASRMETLQKFKSGVYHVLIATDVAARG
Sbjct: 481 LVFASKKATVDEVESQLLQKGFKVAALHGDKDQASRMETLQKFKSGVYHVLIATDVAARG 540

Query: 541 LDIKSIKSVVNFDIAKDMDMHVHRIGRTGRAGDKDGRAHTLITQKEARFAGDLVNSLIAA 600
           LDIKSIKSVVNFDIAKDMDMHVHRIGRTGRAGDKDGRAHTLITQKEARFAG+LVNSLIAA
Sbjct: 541 LDIKSIKSVVNFDIAKDMDMHVHRIGRTGRAGDKDGRAHTLITQKEARFAGELVNSLIAA 600

Query: 601 GQNVSVELMDLAMKDGRFRSKRDARKKGGGGGGGKKGKGRGGNGRGVRGVDFGLGIGYNP 660
           GQNVSVELMDLAMKDGRFRSKRDARKKGGGGGGGKKGK RG NGRGVRGVDFGLGIGYNP
Sbjct: 601 GQNVSVELMDLAMKDGRFRSKRDARKKGGGGGGGKKGKSRGSNGRGVRGVDFGLGIGYNP 660

Query: 661 ESSGSSPSTNNVQSRSAAVNSLRTGMIAQFKSNFVAASSAPPSQGSNNSYSVPANKRPTL 720
           E SGS+PST NVQSRSAAVNSLRTGM+AQFKSNFVAASSAPPSQGS+NSY++PANKRPTL
Sbjct: 661 EGSGSTPSTTNVQSRSAAVNSLRTGMMAQFKSNFVAASSAPPSQGSSNSYNMPANKRPTL 720

Query: 721 AGFVSGGSIGGGANSPAPPVPRANSYVPNAGEYSSQKNSESSGDRPRERRRPSGWDR 778
           +GFVSGGSIGGGANSPAPPV R NSY+PN  EYSSQKN+ESS DRPRER+RPSGWDR
Sbjct: 721 SGFVSGGSIGGGANSPAPPVSRGNSYMPNPVEYSSQKNTESSSDRPRERKRPSGWDR 776

BLAST of Lag0031060 vs. ExPASy TrEMBL
Match: A0A6J1GA90 (DEAD-box ATP-dependent RNA helicase 24 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111452364 PE=3 SV=1)

HSP 1 Score: 1431.4 bits (3704), Expect = 0.0e+00
Identity = 743/778 (95.50%), Postives = 762/778 (97.94%), Query Frame = 0

Query: 1   MSKRKFGFEGFGINRQTTYNFERSQAPQRLYVPPSSRGHGHDNYEDTDVDNIEYDDNETE 60
           MSKRKFGFEGFGINRQTTYNFERSQAPQRLYVPPSSRGHGHDNYEDTDVDNIEYDDNE E
Sbjct: 1   MSKRKFGFEGFGINRQTTYNFERSQAPQRLYVPPSSRGHGHDNYEDTDVDNIEYDDNEAE 60

Query: 61  EASGNGEAGGGAAGGGEEEEIDPLDAFMEGIHEEMRAPPPPKPKEKVDKYRDDEEDDPME 120
           EASGNG+   G AGGGEEEEIDPLDAFMEGIHEEMRAPPPPKPKEKVDKYRD+EEDDPME
Sbjct: 61  EASGNGDNVAG-AGGGEEEEIDPLDAFMEGIHEEMRAPPPPKPKEKVDKYRDEEEDDPME 120

Query: 121 SFLRAKKDVGLTLAADALHAGYDSDEEVYAAAKAVDAGMVEYDSDDNMLILEKKKIEPIP 180
           SFL+AKKDVGLTLAADALHAGYDSDEEVYAAAKAVDA MVEYDSDDNMLI+EKKKIE IP
Sbjct: 121 SFLKAKKDVGLTLAADALHAGYDSDEEVYAAAKAVDAAMVEYDSDDNMLIVEKKKIESIP 180

Query: 181 ALDHSSIDYEPFNKDFYEEKASISGMSEEEVSEYRKSLAIRVSGFDVPRPIKTFEDCGFS 240
           AL+HSSIDYEPFNKDFYEEKASISGMSEEEVSEYRK+LAIRVSGFDVPRPIKTFED GFS
Sbjct: 181 ALEHSSIDYEPFNKDFYEEKASISGMSEEEVSEYRKNLAIRVSGFDVPRPIKTFEDSGFS 240

Query: 241 PQLMNAIKKQGYEKPTSIQCQAMPIVLSGRDIIGIAKTGSGKTAAFVLPMIVHIMDQPEL 300
           PQLMNAIKKQGYEKPTSIQCQAMPIVLSGRDIIGIAKTGSGKTAAFVLPMIVHIMDQPEL
Sbjct: 241 PQLMNAIKKQGYEKPTSIQCQAMPIVLSGRDIIGIAKTGSGKTAAFVLPMIVHIMDQPEL 300

Query: 301 EKEEGPIGVICAPTRELAHQIYLECKKFSKAHGLRVSAVYGGMSKFDQLKELKAGCEIVV 360
           EKEEGPIGVICAPTRELAHQIYLECKKFSK HGLRVSAVYGGMSKFDQ+KELKAGCEIVV
Sbjct: 301 EKEEGPIGVICAPTRELAHQIYLECKKFSKVHGLRVSAVYGGMSKFDQIKELKAGCEIVV 360

Query: 361 ATPGRLIDMIKMKALTMSKATYLVLDEADRMFDLGFEPQIRSIVGQIRPDRQTLLFSATM 420
           ATPGRLIDMIKMKALTMSKATYLVLDEADRMFDLGFEPQIRSIVGQIRPDRQTLLFSATM
Sbjct: 361 ATPGRLIDMIKMKALTMSKATYLVLDEADRMFDLGFEPQIRSIVGQIRPDRQTLLFSATM 420

Query: 421 PRKIEKLAREILTDPVRVTVGEVGMANEDITQVVHVLPSDLEKLPWLLEKLPEMIDDGDV 480
           PRK+EKLAREILTDPVRVTVGEVGMANEDITQVVHVLPSDLEKLPWLLEKLPEMIDDGDV
Sbjct: 421 PRKVEKLAREILTDPVRVTVGEVGMANEDITQVVHVLPSDLEKLPWLLEKLPEMIDDGDV 480

Query: 481 LVFASKKATVDEIESQLQQKGFKVAALHGDKDQASRMETLQKFKSGVYHVLIATDVAARG 540
           LVFASKKATVDEIESQL QKGFKVAALHGDKDQASRMETLQKFKSGVYH LIATDVAARG
Sbjct: 481 LVFASKKATVDEIESQLLQKGFKVAALHGDKDQASRMETLQKFKSGVYHALIATDVAARG 540

Query: 541 LDIKSIKSVVNFDIAKDMDMHVHRIGRTGRAGDKDGRAHTLITQKEARFAGDLVNSLIAA 600
           LDIKSIKSVVNFDIAKDMDMHVHRIGRTGRAGD+DG+AHTLITQKEARFAG+LVNSLIAA
Sbjct: 541 LDIKSIKSVVNFDIAKDMDMHVHRIGRTGRAGDRDGKAHTLITQKEARFAGELVNSLIAA 600

Query: 601 GQNVSVELMDLAMKDGRFRSKRDARKKGGGGGGGKKGKGR-GGNGRGVRGVDFGLGIGYN 660
           GQNVS+ELMDLAMKDGRFRSKRDARKKGGGGGGGKKGKGR GGNGRGVRGVDFGLGIGYN
Sbjct: 601 GQNVSMELMDLAMKDGRFRSKRDARKKGGGGGGGKKGKGRGGGNGRGVRGVDFGLGIGYN 660

Query: 661 PESSGSSPSTNNVQSRSAAVNSLRTGMIAQFKSNFVAASSAPPSQGSNNSYSVPANKRPT 720
           PESSGS+PSTNNVQSRSAAVNSLRTGM+AQFKS+FVAASSAPPSQGSN+SYS+PANKRPT
Sbjct: 661 PESSGSNPSTNNVQSRSAAVNSLRTGMMAQFKSSFVAASSAPPSQGSNSSYSMPANKRPT 720

Query: 721 LAGFVSGGSIGGGANSPAPPVPRANSYVPNAGEYSSQKNSESSGDRPRERRRPSGWDR 778
           LAGFVSGGSIGGGAN P PP PRANSYVPNAGE+SSQKNSESSGDR +ERRRPSGWDR
Sbjct: 721 LAGFVSGGSIGGGANRPPPPPPRANSYVPNAGEFSSQKNSESSGDRSKERRRPSGWDR 777

BLAST of Lag0031060 vs. ExPASy TrEMBL
Match: A0A6J1KCD6 (DEAD-box ATP-dependent RNA helicase 24 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111493042 PE=3 SV=1)

HSP 1 Score: 1426.4 bits (3691), Expect = 0.0e+00
Identity = 740/778 (95.12%), Postives = 761/778 (97.81%), Query Frame = 0

Query: 1   MSKRKFGFEGFGINRQTTYNFERSQAPQRLYVPPSSRGHGHDNYEDTDVDNIEYDDNETE 60
           MSKRKFGFEGFGINRQTTYNFERSQAPQRLYVPPSSRGHGHDNYEDTDVDNIEYDDNE E
Sbjct: 1   MSKRKFGFEGFGINRQTTYNFERSQAPQRLYVPPSSRGHGHDNYEDTDVDNIEYDDNEAE 60

Query: 61  EASGNGEAGGGAAGGGEEEEIDPLDAFMEGIHEEMRAPPPPKPKEKVDKYRDDEEDDPME 120
           EASGNG+   G AGGGEEEEIDPLDAFMEGIHEEM+APPPPKPKEKVDKYRDDEEDDPME
Sbjct: 61  EASGNGDNVAG-AGGGEEEEIDPLDAFMEGIHEEMKAPPPPKPKEKVDKYRDDEEDDPME 120

Query: 121 SFLRAKKDVGLTLAADALHAGYDSDEEVYAAAKAVDAGMVEYDSDDNMLILEKKKIEPIP 180
           SFL+AKKDVGLTLAADALHAGYDSDEEVYAAAKAVDA MVEYDSDDNMLI+EKKKIE IP
Sbjct: 121 SFLKAKKDVGLTLAADALHAGYDSDEEVYAAAKAVDAAMVEYDSDDNMLIVEKKKIESIP 180

Query: 181 ALDHSSIDYEPFNKDFYEEKASISGMSEEEVSEYRKSLAIRVSGFDVPRPIKTFEDCGFS 240
           AL+HSSIDYEPFNKDFYEEKASISGMSEEEVSEYRK+LAIRVSGFDVPRPIKTFED GFS
Sbjct: 181 ALEHSSIDYEPFNKDFYEEKASISGMSEEEVSEYRKNLAIRVSGFDVPRPIKTFEDSGFS 240

Query: 241 PQLMNAIKKQGYEKPTSIQCQAMPIVLSGRDIIGIAKTGSGKTAAFVLPMIVHIMDQPEL 300
            QLMNAIKKQGYEKPTSIQCQAMPIVLSGRDIIGIAKTGSGKTA+FVLPMIVHIMDQPEL
Sbjct: 241 LQLMNAIKKQGYEKPTSIQCQAMPIVLSGRDIIGIAKTGSGKTASFVLPMIVHIMDQPEL 300

Query: 301 EKEEGPIGVICAPTRELAHQIYLECKKFSKAHGLRVSAVYGGMSKFDQLKELKAGCEIVV 360
           EKEEGPIGVICAPTRELAHQIYLECKKFSK HGLRVSAVYGGMSKFDQ+KELKAGCEIVV
Sbjct: 301 EKEEGPIGVICAPTRELAHQIYLECKKFSKVHGLRVSAVYGGMSKFDQIKELKAGCEIVV 360

Query: 361 ATPGRLIDMIKMKALTMSKATYLVLDEADRMFDLGFEPQIRSIVGQIRPDRQTLLFSATM 420
           ATPGRLIDMIKMKALTMSKATYLVLDEADRMFDLGFEPQIRSIVGQIRPDRQTLLFSATM
Sbjct: 361 ATPGRLIDMIKMKALTMSKATYLVLDEADRMFDLGFEPQIRSIVGQIRPDRQTLLFSATM 420

Query: 421 PRKIEKLAREILTDPVRVTVGEVGMANEDITQVVHVLPSDLEKLPWLLEKLPEMIDDGDV 480
           PRK+EKLAREILTDPVRVTVGEVGMANEDITQVVHVLPSDLEKLPWLLEKLPEMIDDGDV
Sbjct: 421 PRKVEKLAREILTDPVRVTVGEVGMANEDITQVVHVLPSDLEKLPWLLEKLPEMIDDGDV 480

Query: 481 LVFASKKATVDEIESQLQQKGFKVAALHGDKDQASRMETLQKFKSGVYHVLIATDVAARG 540
           LVFASKKATVDEIESQL QKGFKVAALHGDKDQASRMETLQKFKSGVYH LIATDVAARG
Sbjct: 481 LVFASKKATVDEIESQLLQKGFKVAALHGDKDQASRMETLQKFKSGVYHALIATDVAARG 540

Query: 541 LDIKSIKSVVNFDIAKDMDMHVHRIGRTGRAGDKDGRAHTLITQKEARFAGDLVNSLIAA 600
           LDIKSIKSVVNFDIAKDMDMHVHRIGRTGRAGD+DG+AHTLITQKEARFAG+LVNSLIAA
Sbjct: 541 LDIKSIKSVVNFDIAKDMDMHVHRIGRTGRAGDRDGKAHTLITQKEARFAGELVNSLIAA 600

Query: 601 GQNVSVELMDLAMKDGRFRSKRDARKKGGGGGGGKKGKGR-GGNGRGVRGVDFGLGIGYN 660
           GQNVS+ELMDLAMKDGRFRSKRDARKKGGGGGGGKKGKGR GGNGRGVRGVDFGLGIGYN
Sbjct: 601 GQNVSMELMDLAMKDGRFRSKRDARKKGGGGGGGKKGKGRGGGNGRGVRGVDFGLGIGYN 660

Query: 661 PESSGSSPSTNNVQSRSAAVNSLRTGMIAQFKSNFVAASSAPPSQGSNNSYSVPANKRPT 720
           PESSGS+PSTNNVQSRSAAVNSLRTGM+AQFK++FVAASSAPPSQGSN+SYS+PANKRPT
Sbjct: 661 PESSGSNPSTNNVQSRSAAVNSLRTGMMAQFKNSFVAASSAPPSQGSNSSYSMPANKRPT 720

Query: 721 LAGFVSGGSIGGGANSPAPPVPRANSYVPNAGEYSSQKNSESSGDRPRERRRPSGWDR 778
           LAGFVSGGSIGGGAN P PP PRANSYVPNAGE+SSQKNSESSGDR +ERRRPSGWDR
Sbjct: 721 LAGFVSGGSIGGGANRPPPPAPRANSYVPNAGEFSSQKNSESSGDRSKERRRPSGWDR 777

BLAST of Lag0031060 vs. ExPASy TrEMBL
Match: A0A6J1GB10 (DEAD-box ATP-dependent RNA helicase 24 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111452364 PE=3 SV=1)

HSP 1 Score: 1426.4 bits (3691), Expect = 0.0e+00
Identity = 743/780 (95.26%), Postives = 762/780 (97.69%), Query Frame = 0

Query: 1   MSKRKFGFEGFGINRQTTYNFERSQAPQRLYVPPSSRGHGHDNYEDTDVDNIEYDDNETE 60
           MSKRKFGFEGFGINRQTTYNFERSQAPQRLYVPPSSRGHGHDNYEDTDVDNIEYDDNE E
Sbjct: 1   MSKRKFGFEGFGINRQTTYNFERSQAPQRLYVPPSSRGHGHDNYEDTDVDNIEYDDNEAE 60

Query: 61  EASGNGEAGGGAAGGGEEEEIDPLDAFMEGIHEEMRAPPPPKPKEKVDKYRDDEEDDPME 120
           EASGNG+   G AGGGEEEEIDPLDAFMEGIHEEMRAPPPPKPKEKVDKYRD+EEDDPME
Sbjct: 61  EASGNGDNVAG-AGGGEEEEIDPLDAFMEGIHEEMRAPPPPKPKEKVDKYRDEEEDDPME 120

Query: 121 SFLRAKKDVGLTLAADALHAGYDSDEEVYAAAKAVDAGMVEYDSDDNMLILEKKKIEPIP 180
           SFL+AKKDVGLTLAADALHAGYDSDEEVYAAAKAVDA MVEYDSDDNMLI+EKKKIE IP
Sbjct: 121 SFLKAKKDVGLTLAADALHAGYDSDEEVYAAAKAVDAAMVEYDSDDNMLIVEKKKIESIP 180

Query: 181 ALDHSSIDYEPFNKDFYEEKASISGMSEEEVSEYRKSLAIRVSGFDVPRPIKTFEDCGFS 240
           AL+HSSIDYEPFNKDFYEEKASISGMSEEEVSEYRK+LAIRVSGFDVPRPIKTFED GFS
Sbjct: 181 ALEHSSIDYEPFNKDFYEEKASISGMSEEEVSEYRKNLAIRVSGFDVPRPIKTFEDSGFS 240

Query: 241 PQLMNAIKKQGYEKPTSIQCQAMPIVLSGRDIIGIAKTGSGKTAAFVLPMIVHIMDQPEL 300
           PQLMNAIKKQGYEKPTSIQCQAMPIVLSGRDIIGIAKTGSGKTAAFVLPMIVHIMDQPEL
Sbjct: 241 PQLMNAIKKQGYEKPTSIQCQAMPIVLSGRDIIGIAKTGSGKTAAFVLPMIVHIMDQPEL 300

Query: 301 EKEEGPIGVICAPTRELAHQIYLECKKFSKAHGLRVSAVYGGMSKFDQLKELKAGCEIVV 360
           EKEEGPIGVICAPTRELAHQIYLECKKFSK HGLRVSAVYGGMSKFDQ+KELKAGCEIVV
Sbjct: 301 EKEEGPIGVICAPTRELAHQIYLECKKFSKVHGLRVSAVYGGMSKFDQIKELKAGCEIVV 360

Query: 361 ATPGRLIDMIKMKALTMSKATYLVLDEADRMFDLGFEPQIRSIVGQIRPDRQTLLFSATM 420
           ATPGRLIDMIKMKALTMSKATYLVLDEADRMFDLGFEPQIRSIVGQIRPDRQTLLFSATM
Sbjct: 361 ATPGRLIDMIKMKALTMSKATYLVLDEADRMFDLGFEPQIRSIVGQIRPDRQTLLFSATM 420

Query: 421 PRKIEKLAREILTDPVRVTVGEVGMANEDITQVVHVLPSDLEKLPWLLEKLPEMIDDGDV 480
           PRK+EKLAREILTDPVRVTVGEVGMANEDITQVVHVLPSDLEKLPWLLEKLPEMIDDGDV
Sbjct: 421 PRKVEKLAREILTDPVRVTVGEVGMANEDITQVVHVLPSDLEKLPWLLEKLPEMIDDGDV 480

Query: 481 LVFASKKATVDEIESQLQQKGFKVAALHGDKDQASRMETLQKFKSGVYHVLIATDVAARG 540
           LVFASKKATVDEIESQL QKGFKVAALHGDKDQASRMETLQKFKSGVYH LIATDVAARG
Sbjct: 481 LVFASKKATVDEIESQLLQKGFKVAALHGDKDQASRMETLQKFKSGVYHALIATDVAARG 540

Query: 541 LDIKSIKSVVNFDIAKDMDMHVHRIGRTGRAGDKDGRAHTLITQKEARFAGDLVNSLIAA 600
           LDIKSIKSVVNFDIAKDMDMHVHRIGRTGRAGD+DG+AHTLITQKEARFAG+LVNSLIAA
Sbjct: 541 LDIKSIKSVVNFDIAKDMDMHVHRIGRTGRAGDRDGKAHTLITQKEARFAGELVNSLIAA 600

Query: 601 GQNVSVELMDLAMKDGRFRSKRDARKK--GGGGGGGKKGKGR-GGNGRGVRGVDFGLGIG 660
           GQNVS+ELMDLAMKDGRFRSKRDARKK  GGGGGGGKKGKGR GGNGRGVRGVDFGLGIG
Sbjct: 601 GQNVSMELMDLAMKDGRFRSKRDARKKGGGGGGGGGKKGKGRGGGNGRGVRGVDFGLGIG 660

Query: 661 YNPESSGSSPSTNNVQSRSAAVNSLRTGMIAQFKSNFVAASSAPPSQGSNNSYSVPANKR 720
           YNPESSGS+PSTNNVQSRSAAVNSLRTGM+AQFKS+FVAASSAPPSQGSN+SYS+PANKR
Sbjct: 661 YNPESSGSNPSTNNVQSRSAAVNSLRTGMMAQFKSSFVAASSAPPSQGSNSSYSMPANKR 720

Query: 721 PTLAGFVSGGSIGGGANSPAPPVPRANSYVPNAGEYSSQKNSESSGDRPRERRRPSGWDR 778
           PTLAGFVSGGSIGGGAN P PP PRANSYVPNAGE+SSQKNSESSGDR +ERRRPSGWDR
Sbjct: 721 PTLAGFVSGGSIGGGANRPPPPPPRANSYVPNAGEFSSQKNSESSGDRSKERRRPSGWDR 779

BLAST of Lag0031060 vs. ExPASy TrEMBL
Match: A0A0A0KTB5 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G004270 PE=3 SV=1)

HSP 1 Score: 1423.7 bits (3684), Expect = 0.0e+00
Identity = 736/778 (94.60%), Postives = 759/778 (97.56%), Query Frame = 0

Query: 1   MSKRKFGFEGFGINRQTTYNFERSQAPQRLYVPPSSRGHGHDNYEDTDVDNIEYDDNETE 60
           MSKRKFGFEGFGINRQTTYNFERSQAPQRLYVPPSSRGHGHDNYEDTDVDNIEYDDN+ E
Sbjct: 1   MSKRKFGFEGFGINRQTTYNFERSQAPQRLYVPPSSRGHGHDNYEDTDVDNIEYDDNDGE 60

Query: 61  EASGNGEAGGGAAGGGEEEEIDPLDAFMEGIHEEMRAPPPPKPKEKVDKYRDDEEDDPME 120
           EASGN + G GA+ G EEEEIDPLDAFMEGIHEEM+APPPPKPKEKVDKYRDD+E+DPME
Sbjct: 61  EASGNADGGAGAS-GAEEEEIDPLDAFMEGIHEEMKAPPPPKPKEKVDKYRDDDEEDPME 120

Query: 121 SFLRAKKDVGLTLAADALHAGYDSDEEVYAAAKAVDAGMVEYDSDDNMLILEKKKIEPIP 180
           SFLRAKKDVGLTLAADALHAGYDSDEEVYAAAKAVDAGMVEYDSDDNMLI+EKKKIEPIP
Sbjct: 121 SFLRAKKDVGLTLAADALHAGYDSDEEVYAAAKAVDAGMVEYDSDDNMLIVEKKKIEPIP 180

Query: 181 ALDHSSIDYEPFNKDFYEEKASISGMSEEEVSEYRKSLAIRVSGFDVPRPIKTFEDCGFS 240
           +LDHSSIDYEPFNKDFYEEKASISGMSEEEVSEYRKSLAIRVSGFDVPRP+KTFEDCGFS
Sbjct: 181 SLDHSSIDYEPFNKDFYEEKASISGMSEEEVSEYRKSLAIRVSGFDVPRPVKTFEDCGFS 240

Query: 241 PQLMNAIKKQGYEKPTSIQCQAMPIVLSGRDIIGIAKTGSGKTAAFVLPMIVHIMDQPEL 300
           PQLMNAIKKQGYEKPTSIQCQAMPIVLSG DIIGIAKTGSGKTAAFVLPMIVHIMDQPEL
Sbjct: 241 PQLMNAIKKQGYEKPTSIQCQAMPIVLSGGDIIGIAKTGSGKTAAFVLPMIVHIMDQPEL 300

Query: 301 EKEEGPIGVICAPTRELAHQIYLECKKFSKAHGLRVSAVYGGMSKFDQLKELKAGCEIVV 360
           EKEEGPIGVICAPTRELAHQIYLECKKFSKAHGLRVSAVYGGMSKFDQLKELKAGCEIVV
Sbjct: 301 EKEEGPIGVICAPTRELAHQIYLECKKFSKAHGLRVSAVYGGMSKFDQLKELKAGCEIVV 360

Query: 361 ATPGRLIDMIKMKALTMSKATYLVLDEADRMFDLGFEPQIRSIVGQIRPDRQTLLFSATM 420
           ATPGRLIDMIK+KALTMSKATYLVLDEADRMFDLGFEPQIRSIVGQIRPDRQTLLFSATM
Sbjct: 361 ATPGRLIDMIKLKALTMSKATYLVLDEADRMFDLGFEPQIRSIVGQIRPDRQTLLFSATM 420

Query: 421 PRKIEKLAREILTDPVRVTVGEVGMANEDITQVVHVLPSDLEKLPWLLEKLPEMIDDGDV 480
           PRK+EKLAREILTDPVRVTVGEVGMANEDITQVVHVLPSDLEKLPWLLEKLPEMIDDGDV
Sbjct: 421 PRKVEKLAREILTDPVRVTVGEVGMANEDITQVVHVLPSDLEKLPWLLEKLPEMIDDGDV 480

Query: 481 LVFASKKATVDEIESQLQQKGFKVAALHGDKDQASRMETLQKFKSGVYHVLIATDVAARG 540
           LVFASKKATVDE+ESQL QK FKVAALHGDKDQASRMETLQKFKSGVYHVLIATDVAARG
Sbjct: 481 LVFASKKATVDEVESQLLQKSFKVAALHGDKDQASRMETLQKFKSGVYHVLIATDVAARG 540

Query: 541 LDIKSIKSVVNFDIAKDMDMHVHRIGRTGRAGDKDGRAHTLITQKEARFAGDLVNSLIAA 600
           LDIKSIKSVVNFDIAKDMDMHVHRIGRTGRAGDKDGRAHTLITQKEARFAG+LVNSLIAA
Sbjct: 541 LDIKSIKSVVNFDIAKDMDMHVHRIGRTGRAGDKDGRAHTLITQKEARFAGELVNSLIAA 600

Query: 601 GQNVSVELMDLAMKDGRFRSKRDARKK-GGGGGGGKKGKGRGGNGRGVRGVDFGLGIGYN 660
           GQNVSVELMDLAMKDGRFRSKRDARKK GGGGGGGKKGK RG NGRGVRGVDFGLGIGYN
Sbjct: 601 GQNVSVELMDLAMKDGRFRSKRDARKKGGGGGGGGKKGKSRGSNGRGVRGVDFGLGIGYN 660

Query: 661 PESSGSSPSTNNVQSRSAAVNSLRTGMIAQFKSNFVAASSAPPSQGSNNSYSVPANKRPT 720
           PE +GS+PST NVQSRSAAVNSLRTGM+AQFKSNFVAASSAPP+QGS+NSY+VPANKRPT
Sbjct: 661 PEGTGSTPSTTNVQSRSAAVNSLRTGMMAQFKSNFVAASSAPPNQGSSNSYNVPANKRPT 720

Query: 721 LAGFVSGGSIGGGANSPAPPVPRANSYVPNAGEYSSQKNSESSGDRPRERRRPSGWDR 778
           L+GFVSGGSIGGGANSPAPPV R NSY+PN  EYSSQKN+ESS DRPRER+RPSGWDR
Sbjct: 721 LSGFVSGGSIGGGANSPAPPVSRGNSYMPNPVEYSSQKNTESSSDRPRERKRPSGWDR 777

BLAST of Lag0031060 vs. TAIR 10
Match: AT2G47330.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 1122.5 bits (2902), Expect = 0.0e+00
Identity = 595/780 (76.28%), Postives = 682/780 (87.44%), Query Frame = 0

Query: 1   MSKRKFGFEGFGINRQTTYNFERSQAPQRLYVPPSSRGHGHDNYEDTDVDNIEYDDNETE 60
           MS RKFG EGFGINRQT+Y+FERSQAPQRLYVPPSSR  G DN ED D+DNI+Y +NE  
Sbjct: 1   MSNRKFGMEGFGINRQTSYSFERSQAPQRLYVPPSSR--GGDNSEDADLDNIDYMENEEA 60

Query: 61  EASGNGEAGGGAAGGGEEEEIDPLDAFMEGIHEEMRAPPPPKPKEKVDKYRDDEEDDPME 120
           E     E G  AA GGE +EIDPLDAFMEGIH+EM++ PPPKPKEK+++Y+DD +DDP+E
Sbjct: 61  EEDIE-EGGSAAASGGEVDEIDPLDAFMEGIHQEMKSAPPPKPKEKLERYKDD-DDDPVE 120

Query: 121 SFLRAKKDVGLTLAADALHAGYDSDEEVYAAAKAVDAGMVEYDSDDNMLILEKKKIEPIP 180
           S+L+AKKD+GLTLAADAL+AGY+SDEEVYAAAKAVDAGM++YDSDDN ++++K+KIEPI 
Sbjct: 121 SYLKAKKDLGLTLAADALNAGYNSDEEVYAAAKAVDAGMLDYDSDDNPIVVDKRKIEPIT 180

Query: 181 ALDHSSIDYEPFNKDFYEEKASISGMSEEEVSEYRKSLAIRVSGFDVPRPIKTFEDCGFS 240
           ALDHSSIDYEP NKDFYEE  SISGM+E+E ++YR+ L IRVSGFDV RP+KTFEDCGFS
Sbjct: 181 ALDHSSIDYEPINKDFYEELESISGMTEQETTDYRQRLGIRVSGFDVHRPVKTFEDCGFS 240

Query: 241 PQLMNAIKKQGYEKPTSIQCQAMPIVLSGRDIIGIAKTGSGKTAAFVLPMIVHIMDQPEL 300
            Q+M+AIKKQ YEKPT+IQCQA+PIVLSGRD+IGIAKTGSGKTAAFVLPMIVHIMDQPEL
Sbjct: 241 SQIMSAIKKQAYEKPTAIQCQALPIVLSGRDVIGIAKTGSGKTAAFVLPMIVHIMDQPEL 300

Query: 301 EKEEGPIGVICAPTRELAHQIYLECKKFSKAHGLRVSAVYGGMSKFDQLKELKAGCEIVV 360
           +++EGPIGVICAPTRELAHQI+LE KKFSKA+GLRVSAVYGGMSK +Q KELKAGCEIVV
Sbjct: 301 QRDEGPIGVICAPTRELAHQIFLEAKKFSKAYGLRVSAVYGGMSKHEQFKELKAGCEIVV 360

Query: 361 ATPGRLIDMIKMKALTMSKATYLVLDEADRMFDLGFEPQIRSIVGQIRPDRQTLLFSATM 420
           ATPGRLIDM+KMKALTM +A+YLVLDEADRMFDLGFEPQ+RSIVGQIRPDRQTLLFSATM
Sbjct: 361 ATPGRLIDMLKMKALTMMRASYLVLDEADRMFDLGFEPQVRSIVGQIRPDRQTLLFSATM 420

Query: 421 PRKIEKLAREILTDPVRVTVGEVGMANEDITQVVHVLPSDLEKLPWLLEKLPEMIDDGDV 480
           P K+EKLAREIL+DP+RVTVGEVGMANEDITQVV+V+PSD EKLPWLLEKLP MID+GDV
Sbjct: 421 PWKVEKLAREILSDPIRVTVGEVGMANEDITQVVNVIPSDAEKLPWLLEKLPGMIDEGDV 480

Query: 481 LVFASKKATVDEIESQLQQKGFKVAALHGDKDQASRMETLQKFKSGVYHVLIATDVAARG 540
           LVFASKKATVDEIE+QL    FKVAALHGDKDQASRMETLQKFKSGV+HVLIATDVAARG
Sbjct: 481 LVFASKKATVDEIEAQLTLNSFKVAALHGDKDQASRMETLQKFKSGVHHVLIATDVAARG 540

Query: 541 LDIKSIKSVVNFDIAKDMDMHVHRIGRTGRAGDKDGRAHTLITQKEARFAGDLVNSLIAA 600
           LDIKS+K+VVN+DIAKDMDMHVHRIGRTGRAGD+DG A+TL+TQ+EARFAG+LVNSL+AA
Sbjct: 541 LDIKSLKTVVNYDIAKDMDMHVHRIGRTGRAGDRDGVAYTLVTQREARFAGELVNSLVAA 600

Query: 601 GQNVSVELMDLAMKDGRFRSKRDARKKGGGGGGGKKGKGRGGNGRGVRGVDFGLGIGYNP 660
           GQNV  EL DLAMKDGRF+SKRD RK      GGKKG+G GG  +GVRGVDFGLGIG++ 
Sbjct: 601 GQNVPPELTDLAMKDGRFKSKRDGRK------GGKKGRGGGGGNKGVRGVDFGLGIGFSS 660

Query: 661 ESSGSSPSTNNVQSRSAAVNSLRTGMIAQFKSNFVAASSAPPSQGSNNSYSVPANKRPTL 720
           ESS  +PS+    SRS A+NS+RTG++AQFK++FVAA+   PS   N +Y    NKRP+L
Sbjct: 661 ESS-RTPSSKAAPSRSGAINSVRTGVMAQFKNSFVAAT---PSNPQNQAY---PNKRPSL 720

Query: 721 AGFVSGGSIGGG---ANSPAPPV-PRANSYVPNAGEYSSQKNSESSGDRPRERRRPSGWD 777
            GFVSGG+IGG      S APPV P  N+   N    SSQ +S+SS +RPRER+R SGWD
Sbjct: 721 MGFVSGGTIGGDMGRTQSQAPPVAPTQNASSHN----SSQNHSQSSENRPRERKRRSGWD 759

BLAST of Lag0031060 vs. TAIR 10
Match: AT1G20920.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 427.9 bits (1099), Expect = 1.7e-119
Identity = 293/707 (41.44%), Postives = 402/707 (56.86%), Query Frame = 0

Query: 56   DNETEEASGNGEAGGGAAGGGEEEEIDPLDAFME-----GIHEEMRAPPPPKPKEKVDKY 115
            D E E A+   E+GG   G  +EEEIDPLDAFM       + +     PPP     V+  
Sbjct: 357  DLENETAATVSESGGD--GAVDEEEIDPLDAFMNTMVLPEVEKFCNGAPPP----AVNDG 416

Query: 116  RDDEEDDPMESFLRAKKDVGLTLAADALHAGYDSDEEVYAAAKAVDAGMVEYDSDDNMLI 175
              D + +  ES  R KK  G   A   +  G DSD + Y+  K  D   ++ D ++ M  
Sbjct: 417  TLDSKMNGKESGDRPKK--GFNKALGRIIQGEDSDSD-YSEPKNDDDPSLDEDDEEFMKR 476

Query: 176  LEKKKIEPIPALDHSSIDYEPFNKDFYEEKASISGMSEEEVSEYRKSLAIRVSGFDVPRP 235
            ++K K E +  +DHS I+YEPF K+FY E   IS M++EEV+ YRK L ++V G DVPRP
Sbjct: 477  VKKTKAEKLSLVDHSKIEYEPFRKNFYIEVKDISRMTQEEVNTYRKELELKVHGKDVPRP 536

Query: 236  IKTFEDCGFSPQLMNAIKKQGYEKPTSIQCQAMPIVLSGRDIIGIAKTGSGKTAAFVLPM 295
            IK +   G + ++++ +KK  YEKP  IQ QA+PI++SGRD IG+AKTGSGKT  FVLPM
Sbjct: 537  IKFWHQTGLTSKILDTMKKLNYEKPMPIQTQALPIIMSGRDCIGVAKTGSGKTLGFVLPM 596

Query: 296  IVHIMDQPELEKEEGPIGVICAPTRELAHQIYLECKKFSKAHGLRVSAVYGGMSKFDQLK 355
            + HI DQP +E  +GPIG++ APTREL  QI+ + +KFSK  G+R   VYGG     Q+ 
Sbjct: 597  LRHIKDQPPVEAGDGPIGLVMAPTRELVQQIHSDIRKFSKPLGIRCVPVYGGSGVAQQIS 656

Query: 356  ELKAGCEIVVATPGRLIDMI---KMKALTMSKATYLVLDEADRMFDLGFEPQIRSIVGQI 415
            ELK G EIVV TPGR+ID++     K   + + T+LV+DEADRMFD+GFEPQI  I+  I
Sbjct: 657  ELKRGTEIVVCTPGRMIDILCTSSGKITNLRRVTFLVMDEADRMFDMGFEPQITRIIQNI 716

Query: 416  RPDRQTLLFSATMPRKIEKLAREILTDPVRVTVGEVGMANEDITQVVHVLPSDLEKLPWL 475
            RP+RQT+LFSAT PR++E LAR++L  PV + VG   + N+DITQ+V V P + ++   L
Sbjct: 717  RPERQTVLFSATFPRQVETLARKVLNKPVEIQVGGRSVVNKDITQLVEVRP-ESDRFLRL 776

Query: 476  LEKLPEMIDDGDVLVFASKKATVDEIESQLQQKGFKVAALHGDKDQASRMETLQKFKSGV 535
            LE L E  + G +LVF   +   D +   + +  +   +LHG KDQ  R  T+  FK+ V
Sbjct: 777  LELLGEWSEKGKILVFVQSQEKCDALYRDMIKSSYPCLSLHGGKDQTDRESTISDFKNDV 836

Query: 536  YHVLIATDVAARGLDIKSIKSVVNFDIAKDMDMHVHRIGRTGRAGDKDGRAHTLITQKEA 595
             ++LIAT VAARGLD+K ++ VVNFD     + +VHR+GRTGRAG K G A T I++ +A
Sbjct: 837  CNLLIATSVAARGLDVKELELVVNFDAPNHYEDYVHRVGRTGRAGRK-GCAVTFISEDDA 896

Query: 596  RFAGDLVNSLIAAGQNVSVELMDLAMKDG-RFRSKRDARKKGGGGGGGKKGKGRGGNGRG 655
            ++A DLV +L  + Q V  +L  LA  DG   + K+   +  G G GG   K        
Sbjct: 897  KYAPDLVKALELSEQPVPDDLKALA--DGFMVKVKQGIEQAHGTGYGGSGFKFNEEEEEV 956

Query: 656  VRGVDFGLGIGYNPESSGSSPSTNNVQSRSAAVNSLRTGMIAQFKSNF--VAASSAPPSQ 715
             +         Y  E   S     N   R A       G I+Q ++ F  +AA +A    
Sbjct: 957  RKAAKKAQAKEYGFEEDKSDSEDENDVVRKAG-----GGEISQQQATFAQIAAIAAAAKA 1016

Query: 716  GSNNSYSVPANKRPTLAGFVSGGSIGGGANSPAPPVPRANSYVPNAG 752
             +    S P      LA        GGG  +    +P     +P+ G
Sbjct: 1017 AAAAPVSAPVTANQLLAN-------GGGLAAMPGVLPVTVPTLPSEG 1038

BLAST of Lag0031060 vs. TAIR 10
Match: AT1G20920.2 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 427.9 bits (1099), Expect = 1.7e-119
Identity = 293/707 (41.44%), Postives = 402/707 (56.86%), Query Frame = 0

Query: 56  DNETEEASGNGEAGGGAAGGGEEEEIDPLDAFME-----GIHEEMRAPPPPKPKEKVDKY 115
           D E E A+   E+GG   G  +EEEIDPLDAFM       + +     PPP     V+  
Sbjct: 19  DLENETAATVSESGGD--GAVDEEEIDPLDAFMNTMVLPEVEKFCNGAPPP----AVNDG 78

Query: 116 RDDEEDDPMESFLRAKKDVGLTLAADALHAGYDSDEEVYAAAKAVDAGMVEYDSDDNMLI 175
             D + +  ES  R KK  G   A   +  G DSD + Y+  K  D   ++ D ++ M  
Sbjct: 79  TLDSKMNGKESGDRPKK--GFNKALGRIIQGEDSDSD-YSEPKNDDDPSLDEDDEEFMKR 138

Query: 176 LEKKKIEPIPALDHSSIDYEPFNKDFYEEKASISGMSEEEVSEYRKSLAIRVSGFDVPRP 235
           ++K K E +  +DHS I+YEPF K+FY E   IS M++EEV+ YRK L ++V G DVPRP
Sbjct: 139 VKKTKAEKLSLVDHSKIEYEPFRKNFYIEVKDISRMTQEEVNTYRKELELKVHGKDVPRP 198

Query: 236 IKTFEDCGFSPQLMNAIKKQGYEKPTSIQCQAMPIVLSGRDIIGIAKTGSGKTAAFVLPM 295
           IK +   G + ++++ +KK  YEKP  IQ QA+PI++SGRD IG+AKTGSGKT  FVLPM
Sbjct: 199 IKFWHQTGLTSKILDTMKKLNYEKPMPIQTQALPIIMSGRDCIGVAKTGSGKTLGFVLPM 258

Query: 296 IVHIMDQPELEKEEGPIGVICAPTRELAHQIYLECKKFSKAHGLRVSAVYGGMSKFDQLK 355
           + HI DQP +E  +GPIG++ APTREL  QI+ + +KFSK  G+R   VYGG     Q+ 
Sbjct: 259 LRHIKDQPPVEAGDGPIGLVMAPTRELVQQIHSDIRKFSKPLGIRCVPVYGGSGVAQQIS 318

Query: 356 ELKAGCEIVVATPGRLIDMI---KMKALTMSKATYLVLDEADRMFDLGFEPQIRSIVGQI 415
           ELK G EIVV TPGR+ID++     K   + + T+LV+DEADRMFD+GFEPQI  I+  I
Sbjct: 319 ELKRGTEIVVCTPGRMIDILCTSSGKITNLRRVTFLVMDEADRMFDMGFEPQITRIIQNI 378

Query: 416 RPDRQTLLFSATMPRKIEKLAREILTDPVRVTVGEVGMANEDITQVVHVLPSDLEKLPWL 475
           RP+RQT+LFSAT PR++E LAR++L  PV + VG   + N+DITQ+V V P + ++   L
Sbjct: 379 RPERQTVLFSATFPRQVETLARKVLNKPVEIQVGGRSVVNKDITQLVEVRP-ESDRFLRL 438

Query: 476 LEKLPEMIDDGDVLVFASKKATVDEIESQLQQKGFKVAALHGDKDQASRMETLQKFKSGV 535
           LE L E  + G +LVF   +   D +   + +  +   +LHG KDQ  R  T+  FK+ V
Sbjct: 439 LELLGEWSEKGKILVFVQSQEKCDALYRDMIKSSYPCLSLHGGKDQTDRESTISDFKNDV 498

Query: 536 YHVLIATDVAARGLDIKSIKSVVNFDIAKDMDMHVHRIGRTGRAGDKDGRAHTLITQKEA 595
            ++LIAT VAARGLD+K ++ VVNFD     + +VHR+GRTGRAG K G A T I++ +A
Sbjct: 499 CNLLIATSVAARGLDVKELELVVNFDAPNHYEDYVHRVGRTGRAGRK-GCAVTFISEDDA 558

Query: 596 RFAGDLVNSLIAAGQNVSVELMDLAMKDG-RFRSKRDARKKGGGGGGGKKGKGRGGNGRG 655
           ++A DLV +L  + Q V  +L  LA  DG   + K+   +  G G GG   K        
Sbjct: 559 KYAPDLVKALELSEQPVPDDLKALA--DGFMVKVKQGIEQAHGTGYGGSGFKFNEEEEEV 618

Query: 656 VRGVDFGLGIGYNPESSGSSPSTNNVQSRSAAVNSLRTGMIAQFKSNF--VAASSAPPSQ 715
            +         Y  E   S     N   R A       G I+Q ++ F  +AA +A    
Sbjct: 619 RKAAKKAQAKEYGFEEDKSDSEDENDVVRKAG-----GGEISQQQATFAQIAAIAAAAKA 678

Query: 716 GSNNSYSVPANKRPTLAGFVSGGSIGGGANSPAPPVPRANSYVPNAG 752
            +    S P      LA        GGG  +    +P     +P+ G
Sbjct: 679 AAAAPVSAPVTANQLLAN-------GGGLAAMPGVLPVTVPTLPSEG 700

BLAST of Lag0031060 vs. TAIR 10
Match: AT5G63120.2 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 406.0 bits (1042), Expect = 6.9e-113
Identity = 221/456 (48.46%), Postives = 297/456 (65.13%), Query Frame = 0

Query: 192 FNKDFYEEKASISGMSEEEVSEYRKSLAIRVSGFDVPRPIKTFEDCGFSPQLMNAIKKQG 251
           F K+FY E  ++  M+E++V+ YR    I V G DVP+P+K F+D  F   ++ AI K G
Sbjct: 125 FEKNFYVESPTVQAMTEQDVAMYRTERDISVEGRDVPKPMKMFQDANFPDNILEAIAKLG 184

Query: 252 YEKPTSIQCQAMPIVLSGRDIIGIAKTGSGKTAAFVLPMIVHIMDQPELEKEEGPIGVIC 311
           + +PT IQ Q  P+ L GRD+IGIA+TGSGKT A++LP +VH+  QP L +++GPI +I 
Sbjct: 185 FTEPTPIQAQGWPMALKGRDLIGIAETGSGKTLAYLLPALVHVSAQPRLGQDDGPIVLIL 244

Query: 312 APTRELAHQIYLECKKFSKAHGLRVSAVYGGMSKFDQLKELKAGCEIVVATPGRLIDMIK 371
           APTRELA QI  E +KF    G+R + +YGG  K  Q+++L+ G EIV+ATPGRLIDM++
Sbjct: 245 APTRELAVQIQEESRKFGLRSGVRSTCIYGGAPKGPQIRDLRRGVEIVIATPGRLIDMLE 304

Query: 372 MKALTMSKATYLVLDEADRMFDLGFEPQIRSIVGQIRPDRQTLLFSATMPRKIEKLAREI 431
            +   + + TYLVLDEADRM D+GFEPQIR IV QIRPDRQTLL+SAT PR++E LAR+ 
Sbjct: 305 CQHTNLKRVTYLVLDEADRMLDMGFEPQIRKIVSQIRPDRQTLLWSATWPREVETLARQF 364

Query: 432 LTDPVRVTVGEVGM-ANEDITQVVHVLPSDLEKLPWLLEKLPEMIDDGDVLVFASKKATV 491
           L DP +  +G   + AN+ I QV+ ++P+  EK   LL  L +++D   +L+F   K   
Sbjct: 365 LRDPYKAIIGSTDLKANQSINQVIEIVPTP-EKYNRLLTLLKQLMDGSKILIFVETKRGC 424

Query: 492 DEIESQLQQKGFKVAALHGDKDQASRMETLQKFKSGVYHVLIATDVAARGLDIKSIKSVV 551
           D++  QL+  G+   A+HGDK Q+ R   L +FKSG   ++ ATDVAARGLD+K IK VV
Sbjct: 425 DQVTRQLRMDGWPALAIHGDKTQSERDRVLAEFKSGRSPIMTATDVAARGLDVKDIKCVV 484

Query: 552 NFDIAKDMDMHVHRIGRTGRAGDKDGRAHTLITQKEARFAGDLVNSLIAAGQNVSVELMD 611
           N+D    ++ ++HRIGRTGRAG K G A T  T   A+FA +LV  L  AGQ V   L  
Sbjct: 485 NYDFPNTLEDYIHRIGRTGRAGAK-GMAFTFFTHDNAKFARELVKILQEAGQVVPPTLSA 544

Query: 612 LAMKDGRFRSKRDARKKGGGGGGGKKGKGRGGNGRG 647
           L    G            GG GGG+  + RGG GRG
Sbjct: 545 LVRSSG---------SGYGGSGGGRNFRPRGG-GRG 568

BLAST of Lag0031060 vs. TAIR 10
Match: AT3G09620.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 395.6 bits (1015), Expect = 9.3e-110
Identity = 272/651 (41.78%), Postives = 373/651 (57.30%), Query Frame = 0

Query: 57  NETEEASGNGEAGGGAAGGGEEEEIDPLDAFMEGIHEEMRAPPPPKPKEKVDKYRDDEED 116
           +E E A    E GG  A   +E+EIDPLDAFM      M  P   K    V     D + 
Sbjct: 229 SENETAVTVSENGGDRA--ADEDEIDPLDAFM----NTMVLPEVEKLSNIVIDGILDFKM 288

Query: 117 DPMESFLRAKKDVGLTLAADALHAGYDSDEEVYAAAKAVDAGMVEYDSDDNMLILEKKKI 176
           +  E+  +AKK      A   +  G DSD + Y+  K+ D   ++ D ++ M  ++K K 
Sbjct: 289 NGKETGDQAKKGFN-KAALGRIIQGEDSDSD-YSEPKSDDDPSLDEDDEEFMKRVKKTKA 348

Query: 177 EPIPALDHSSIDYEPFNKDFYEEKASISGMSEEEVSEYRKSLAIRVSGFDVPRPIKTFED 236
           E +  +DHS I+YEPF K+FY E   IS M+++ V+ YRK L ++V G DVPRPI+ +  
Sbjct: 349 EKLSLVDHSKIEYEPFRKNFYIEVKDISRMTQDAVNAYRKELELKVHGKDVPRPIQFWHQ 408

Query: 237 CGFSPQLMNAIKKQGYEKPTSIQCQAMPIVLSGRDIIGIAKTGSGKTAAFVLPMIVHIMD 296
            G + ++++ +KK  YEKP  IQ QA+PI++SGRD IG+AKTGSGKT  FVLPM+ HI D
Sbjct: 409 TGLTSKILDTLKKLNYEKPMPIQAQALPIIMSGRDCIGVAKTGSGKTLGFVLPMLRHIKD 468

Query: 297 QPELEKEEGPIGVICAPTRELAHQIYLECKKFSKAHGLRVSAVYGGMSKFDQLKELKAGC 356
           QP +E  +GPIG++ APTREL  QIY + +KFSKA G+    VYGG     Q+ ELK G 
Sbjct: 469 QPPVEAGDGPIGLVMAPTRELVQQIYSDIRKFSKALGIICVPVYGGSGVAQQISELKRGT 528

Query: 357 EIVVATPGRLIDMI---KMKALTMSKATYLVLDEADRMFDLGFEPQIRSIVGQIRPDRQT 416
           EIVV TPGR+ID++     K   + + TYLV+DEADRMFD+GFEPQI  IV  IRPDRQT
Sbjct: 529 EIVVCTPGRMIDILCTSSGKITNLRRVTYLVMDEADRMFDMGFEPQITRIVQNIRPDRQT 588

Query: 417 LLFSATMPRKIEKLAREILTDPVRVTVGEVGMANEDITQVVHVLPSDLEKLPWLLEKLPE 476
           +LFSAT PR++E LAR++L  PV + VG   + N+DITQ+V + P + E+   LLE L E
Sbjct: 589 VLFSATFPRQVETLARKVLNKPVEIQVGGRSVVNKDITQLVEIRP-ESERFSRLLELLGE 648

Query: 477 MIDDGDVLVFASKKATVDEIESQLQQKGFKVAALHGDKDQASRMETLQKFKSGVYHVLIA 536
             + G VLVF                               S+ +++  FKS V ++LIA
Sbjct: 649 WYEKGKVLVFV-----------------------------RSQEKSISDFKSDVCNLLIA 708

Query: 537 TDVAARGLDIKSIKSVVNFDIAKDMDMHVHRIGRTGRAGDKDGRAHTLITQKEARFAGDL 596
           T VAARGLD+K ++ VVNFD     + +VHR+GRTGRAG K G A T I++ +A++A DL
Sbjct: 709 TSVAARGLDVKELELVVNFDAPNHYEDYVHRVGRTGRAGRK-GCAVTFISEDDAKYAPDL 768

Query: 597 VNSLIAAGQNVSVELMDLAMKDGRFRSKRDARKKGGG---GGGGKKGKGRGGNGRGVRGV 656
           V +L  + Q V  ++   A+ +G     +   ++  G   GG G K        R     
Sbjct: 769 VKALELSEQPVPDDVK--AVAEGFMAKVKQGIEQAHGTGYGGSGFKFNEEEDEVRKAAKK 828

Query: 657 DFGLGIGYNPESSGSSPSTNNVQSRSAAVNSLRTGMIAQFKSNFVAASSAP 702
                 G+  E S  S   N+V  ++    S +   +AQ  +   AAS AP
Sbjct: 829 AQAKEYGFEEEKS-DSEDENDVVRKAGGDISQQQITLAQIAAIASAASKAP 837

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_008454276.10.0e+0095.62PREDICTED: DEAD-box ATP-dependent RNA helicase 24 [Cucumis melo][more]
XP_022948807.10.0e+0095.50DEAD-box ATP-dependent RNA helicase 24 isoform X2 [Cucurbita moschata][more]
XP_023523273.10.0e+0095.27DEAD-box ATP-dependent RNA helicase 24 isoform X2 [Cucurbita pepo subsp. pepo][more]
XP_023523272.10.0e+0095.03DEAD-box ATP-dependent RNA helicase 24 isoform X1 [Cucurbita pepo subsp. pepo][more]
XP_022998395.10.0e+0095.12DEAD-box ATP-dependent RNA helicase 24 isoform X2 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
O229070.0e+0076.28DEAD-box ATP-dependent RNA helicase 24 OS=Arabidopsis thaliana OX=3702 GN=RH24 P... [more]
Q10MH83.8e-31073.58DEAD-box ATP-dependent RNA helicase 24 OS=Oryza sativa subsp. japonica OX=39947 ... [more]
Q810A77.6e-14941.50ATP-dependent RNA helicase DDX42 OS=Mus musculus OX=10090 GN=Ddx42 PE=1 SV=3[more]
Q86XP39.9e-14942.37ATP-dependent RNA helicase DDX42 OS=Homo sapiens OX=9606 GN=DDX42 PE=1 SV=1[more]
Q54IV33.8e-14846.85Probable ATP-dependent RNA helicase ddx42 OS=Dictyostelium discoideum OX=44689 G... [more]
Match NameE-valueIdentityDescription
A0A1S3BY830.0e+0095.62DEAD-box ATP-dependent RNA helicase 24 OS=Cucumis melo OX=3656 GN=LOC103494724 P... [more]
A0A6J1GA900.0e+0095.50DEAD-box ATP-dependent RNA helicase 24 isoform X2 OS=Cucurbita moschata OX=3662 ... [more]
A0A6J1KCD60.0e+0095.12DEAD-box ATP-dependent RNA helicase 24 isoform X2 OS=Cucurbita maxima OX=3661 GN... [more]
A0A6J1GB100.0e+0095.26DEAD-box ATP-dependent RNA helicase 24 isoform X1 OS=Cucurbita moschata OX=3662 ... [more]
A0A0A0KTB50.0e+0094.60Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G004270 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT2G47330.10.0e+0076.28P-loop containing nucleoside triphosphate hydrolases superfamily protein [more]
AT1G20920.11.7e-11941.44P-loop containing nucleoside triphosphate hydrolases superfamily protein [more]
AT1G20920.21.7e-11941.44P-loop containing nucleoside triphosphate hydrolases superfamily protein [more]
AT5G63120.26.9e-11348.46P-loop containing nucleoside triphosphate hydrolases superfamily protein [more]
AT3G09620.19.3e-11041.78P-loop containing nucleoside triphosphate hydrolases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR014001Helicase superfamily 1/2, ATP-binding domainSMARTSM00487ultradead3coord: 251..453
e-value: 1.6E-65
score: 233.7
IPR014001Helicase superfamily 1/2, ATP-binding domainPROSITEPS51192HELICASE_ATP_BIND_1coord: 263..438
score: 33.263187
IPR001650Helicase, C-terminalSMARTSM00490helicmild6coord: 491..572
e-value: 7.0E-32
score: 121.9
IPR001650Helicase, C-terminalPFAMPF00271Helicase_Ccoord: 468..572
e-value: 3.7E-27
score: 94.9
IPR001650Helicase, C-terminalPROSITEPS51194HELICASE_CTERcoord: 463..612
score: 22.061138
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3D3.40.50.300coord: 194..438
e-value: 1.4E-91
score: 308.4
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3D3.40.50.300coord: 439..616
e-value: 1.0E-47
score: 163.7
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILY52540P-loop containing nucleoside triphosphate hydrolasescoord: 378..608
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILY52540P-loop containing nucleoside triphosphate hydrolasescoord: 215..441
IPR011545DEAD/DEAH box helicase domainPFAMPF00270DEADcoord: 256..427
e-value: 2.8E-52
score: 177.0
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 13..31
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 697..715
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 48..62
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..116
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 659..677
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 761..777
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 85..116
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 617..677
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 697..777
NoneNo IPR availablePANTHERPTHR47958ATP-DEPENDENT RNA HELICASE DBP3coord: 46..663
NoneNo IPR availablePANTHERPTHR47958:SF47DEAD-BOX ATP-DEPENDENT RNA HELICASE 24coord: 46..663
NoneNo IPR availableCDDcd17952DEADc_DDX42coord: 243..439
e-value: 4.29935E-132
score: 388.311
NoneNo IPR availableCDDcd18787SF2_C_DEADcoord: 450..582
e-value: 4.03286E-51
score: 172.692
IPR000629ATP-dependent RNA helicase DEAD-box, conserved sitePROSITEPS00039DEAD_ATP_HELICASEcoord: 384..392
IPR014014RNA helicase, DEAD-box type, Q motifPROSITEPS51195Q_MOTIFcoord: 232..260
score: 12.623852

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0031060.1Lag0031060.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005634 nucleus
molecular_function GO:0005524 ATP binding
molecular_function GO:0016787 hydrolase activity
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0003724 RNA helicase activity