Lag0031049 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0031049
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionrRNA adenine N(6)-methyltransferase
Locationchr11: 4206620 .. 4208242 (-)
RNA-Seq ExpressionLag0031049
SyntenyLag0031049
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGGGAGGCAAGATGAAAAGGGAGAAGCCAAAAGCAGCGGCGAAGAGTGCGCCGTATCAGGGAGGGATTTCGTTCCACAAATCCAAGGGTCAGCATATTCTTAAGAATCCCTTACTGGTCGACTCTATAGTTCAAAAGTCTGGAATCAAGAGCACCGATGTCATCCTCGAAATCGGTCCCGGTACTGGTAATCTCACCAAGAAGCTTCTAGAATGCGGTAAGATGGTTATTGCTGTCGAGCTCGATCCTCGTATGGTTCTCGAGTTGCAGAGGCGCTTTCAGGACACTCCCTATTCTTCTCGCTTGAAGGTGGGTTTTCGTTTCGTTTGATCCTTTAGGTTTTTATTGAGTGATTGGTTATCCAAAAATTGGAGTTTCTTTTGTCCATTATTTATCCATCTGCTTCCTATCAATTGGGTTTTGTTTGATGATCTGGTTTAGTTGATATATTGTTCTTGAATCTTCTGCTCATTGCTATTCTGGTTATCCCTCCCTTTTGTTGGAGGAAAAATAATCTCACTAAGACTCAATTTCTTTCAGTTGTATGAAGTACTTGTACCAACATTTAATCTTTCCCTTCTGTAGTAGGCAAAATGTAATTGGGAAGGGAGAAGGATCTTCTTCAAAGTAGTTTTGCTTACCTAAAAAACAGTCCCTTCCTTAATCTGCCCCATTGACTGGTTATTTCCCATCAAATTATCTTAATAACTGTTTGAATGAACCAACCAGAGTGTTCTGTAGTTGCAGATGTGTGCTTGTGCTTGATTAAGTTGGCTGAGAAGTGAGTTTGTACTGGTTATTTTACTTGATTCTTAATTTGATTTTCTCCAATAATTGACTAGGTCTTCAACTTGATGTGCTCAGGTTATTCAAGGAGATGTGCTCAAGACTGAGCTTCCCTATTTTGATATATGTGTGGCCAATATCCCTTATCAAATATCATCTCCTCTCACATTCAAGTTGCTAAATCATCAACCTTCTTTTCGGTGCGCAATCATAATGTTCCAGAGGGAATTTGCAATGAGGCTAGTTGCTCAACCTGGAGACAAACTTTACTGCCGTTTGACTGTAAACACCCAACTCCTAGCTCGAGTATCTCACCTCCTCAAAGTTGGGAAGAACAATTTTCGTCCTCCGCCAAAGGTGGACTCATCTGTAGTTCGAATTGAGCCCAGGAAACCACGTATTGAGGTGAAGCAAAAGGAGTGGGATGGATTTCTGAGAATCTGTTTCAATCGAAAGAACAAAACTCTTGGTTCAATTTTTAGGCAGAAGAGTGTTCTTTCATTGCTAGAGAAGAACTACAAAACATTGTGTGCACTTAAAATCCCTCTGCAAAGTTCTGCAGATAATGATGATGGTGCTTTTGATTATTCGAGATTTGGCGAGTCAAATGAGGATCAAACCATGGAAGTAGACGACGATGGAGCTGACGAGGAGATGGATATGGATGATAGTGATGCTGAAGGTGGGGAAGGGGAGGCTTCTGAATTTAAGGATAAAGTCTTGGGTGTTTTGAAGGAGGGAGATTTTGAAGAAAAAAGGTCGTCTAAACTCACCTTACAGGAGTTTTTATACCTACTCTCATTGTTCAACAAAGCTGGCATACATTTTTCTTGA

mRNA sequence

ATGGCGGGAGGCAAGATGAAAAGGGAGAAGCCAAAAGCAGCGGCGAAGAGTGCGCCGTATCAGGGAGGGATTTCGTTCCACAAATCCAAGGGTCAGCATATTCTTAAGAATCCCTTACTGGTCGACTCTATAGTTCAAAAGTCTGGAATCAAGAGCACCGATGTCATCCTCGAAATCGGTCCCGGTACTGGTAATCTCACCAAGAAGCTTCTAGAATGCGGTAAGATGGTTATTGCTGTCGAGCTCGATCCTCGTATGGTTCTCGAGTTGCAGAGGCGCTTTCAGGACACTCCCTATTCTTCTCGCTTGAAGGTTATTCAAGGAGATGTGCTCAAGACTGAGCTTCCCTATTTTGATATATGTGTGGCCAATATCCCTTATCAAATATCATCTCCTCTCACATTCAAGTTGCTAAATCATCAACCTTCTTTTCGGTGCGCAATCATAATGTTCCAGAGGGAATTTGCAATGAGGCTAGTTGCTCAACCTGGAGACAAACTTTACTGCCGTTTGACTGTAAACACCCAACTCCTAGCTCGAGTATCTCACCTCCTCAAAGTTGGGAAGAACAATTTTCGTCCTCCGCCAAAGGTGGACTCATCTGTAGTTCGAATTGAGCCCAGGAAACCACGTATTGAGGTGAAGCAAAAGGAGTGGGATGGATTTCTGAGAATCTGTTTCAATCGAAAGAACAAAACTCTTGGTTCAATTTTTAGGCAGAAGAGTGTTCTTTCATTGCTAGAGAAGAACTACAAAACATTGTGTGCACTTAAAATCCCTCTGCAAAGTTCTGCAGATAATGATGATGGTGCTTTTGATTATTCGAGATTTGGCGAGTCAAATGAGGATCAAACCATGGAAGTAGACGACGATGGAGCTGACGAGGAGATGGATATGGATGATAGTGATGCTGAAGGTGGGGAAGGGGAGGCTTCTGAATTTAAGGATAAAGTCTTGGGTGTTTTGAAGGAGGGAGATTTTGAAGAAAAAAGGTCGTCTAAACTCACCTTACAGGAGTTTTTATACCTACTCTCATTGTTCAACAAAGCTGGCATACATTTTTCTTGA

Coding sequence (CDS)

ATGGCGGGAGGCAAGATGAAAAGGGAGAAGCCAAAAGCAGCGGCGAAGAGTGCGCCGTATCAGGGAGGGATTTCGTTCCACAAATCCAAGGGTCAGCATATTCTTAAGAATCCCTTACTGGTCGACTCTATAGTTCAAAAGTCTGGAATCAAGAGCACCGATGTCATCCTCGAAATCGGTCCCGGTACTGGTAATCTCACCAAGAAGCTTCTAGAATGCGGTAAGATGGTTATTGCTGTCGAGCTCGATCCTCGTATGGTTCTCGAGTTGCAGAGGCGCTTTCAGGACACTCCCTATTCTTCTCGCTTGAAGGTTATTCAAGGAGATGTGCTCAAGACTGAGCTTCCCTATTTTGATATATGTGTGGCCAATATCCCTTATCAAATATCATCTCCTCTCACATTCAAGTTGCTAAATCATCAACCTTCTTTTCGGTGCGCAATCATAATGTTCCAGAGGGAATTTGCAATGAGGCTAGTTGCTCAACCTGGAGACAAACTTTACTGCCGTTTGACTGTAAACACCCAACTCCTAGCTCGAGTATCTCACCTCCTCAAAGTTGGGAAGAACAATTTTCGTCCTCCGCCAAAGGTGGACTCATCTGTAGTTCGAATTGAGCCCAGGAAACCACGTATTGAGGTGAAGCAAAAGGAGTGGGATGGATTTCTGAGAATCTGTTTCAATCGAAAGAACAAAACTCTTGGTTCAATTTTTAGGCAGAAGAGTGTTCTTTCATTGCTAGAGAAGAACTACAAAACATTGTGTGCACTTAAAATCCCTCTGCAAAGTTCTGCAGATAATGATGATGGTGCTTTTGATTATTCGAGATTTGGCGAGTCAAATGAGGATCAAACCATGGAAGTAGACGACGATGGAGCTGACGAGGAGATGGATATGGATGATAGTGATGCTGAAGGTGGGGAAGGGGAGGCTTCTGAATTTAAGGATAAAGTCTTGGGTGTTTTGAAGGAGGGAGATTTTGAAGAAAAAAGGTCGTCTAAACTCACCTTACAGGAGTTTTTATACCTACTCTCATTGTTCAACAAAGCTGGCATACATTTTTCTTGA

Protein sequence

MAGGKMKREKPKAAAKSAPYQGGISFHKSKGQHILKNPLLVDSIVQKSGIKSTDVILEIGPGTGNLTKKLLECGKMVIAVELDPRMVLELQRRFQDTPYSSRLKVIQGDVLKTELPYFDICVANIPYQISSPLTFKLLNHQPSFRCAIIMFQREFAMRLVAQPGDKLYCRLTVNTQLLARVSHLLKVGKNNFRPPPKVDSSVVRIEPRKPRIEVKQKEWDGFLRICFNRKNKTLGSIFRQKSVLSLLEKNYKTLCALKIPLQSSADNDDGAFDYSRFGESNEDQTMEVDDDGADEEMDMDDSDAEGGEGEASEFKDKVLGVLKEGDFEEKRSSKLTLQEFLYLLSLFNKAGIHFS
Homology
BLAST of Lag0031049 vs. NCBI nr
Match: XP_023525787.1 (ribosomal RNA small subunit methyltransferase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 673.7 bits (1737), Expect = 8.7e-190
Identity = 342/355 (96.34%), Postives = 348/355 (98.03%), Query Frame = 0

Query: 1   MAGGKMKREKPKAAAKSAPYQGGISFHKSKGQHILKNPLLVDSIVQKSGIKSTDVILEIG 60
           MAGGKMKREKPKA+AK+APYQGGISFHKSKGQHILKNP+LVDSIVQKSGIKSTDVILEIG
Sbjct: 1   MAGGKMKREKPKASAKTAPYQGGISFHKSKGQHILKNPMLVDSIVQKSGIKSTDVILEIG 60

Query: 61  PGTGNLTKKLLECGKMVIAVELDPRMVLELQRRFQDTPYSSRLKVIQGDVLKTELPYFDI 120
           PGTGNLTKKLLECGKMVIAVELDPRMVLELQRRFQDTPYSSRLKVIQGDVLKTELPYFDI
Sbjct: 61  PGTGNLTKKLLECGKMVIAVELDPRMVLELQRRFQDTPYSSRLKVIQGDVLKTELPYFDI 120

Query: 121 CVANIPYQISSPLTFKLLNHQPSFRCAIIMFQREFAMRLVAQPGDKLYCRLTVNTQLLAR 180
           CVANIPYQISSPLTFKLLNHQPSFRCAIIMFQREFAMRLVAQPGDKLYCRLTVNTQLL+R
Sbjct: 121 CVANIPYQISSPLTFKLLNHQPSFRCAIIMFQREFAMRLVAQPGDKLYCRLTVNTQLLSR 180

Query: 181 VSHLLKVGKNNFRPPPKVDSSVVRIEPRKPRIEVKQKEWDGFLRICFNRKNKTLGSIFRQ 240
           VSHLLKVGKNNFRPPPKVDSSVVRIEPRKPRIEVKQKEWDGFLRICFNRKNKTLGSIFRQ
Sbjct: 181 VSHLLKVGKNNFRPPPKVDSSVVRIEPRKPRIEVKQKEWDGFLRICFNRKNKTLGSIFRQ 240

Query: 241 KSVLSLLEKNYKTLCALKIPLQSSADNDDGAFDYSRFGESNEDQTMEVDDDGADEEMDMD 300
           KSVLSLLEKNYKTLCAL IP Q SAD DDG FDYSRFG SNEDQTMEVDDDG DEEMDMD
Sbjct: 241 KSVLSLLEKNYKTLCALNIPQQGSADKDDGDFDYSRFGNSNEDQTMEVDDDGGDEEMDMD 300

Query: 301 DSDAEGGEGEASEFKDKVLGVLKEGDFEEKRSSKLTLQEFLYLLSLFNKAGIHFS 356
           DSDAEGGEGEASEFKDKV+GVLKEGDFEEKRSSKL+LQEFLYLLSLFNKAGIHFS
Sbjct: 301 DSDAEGGEGEASEFKDKVMGVLKEGDFEEKRSSKLSLQEFLYLLSLFNKAGIHFS 355

BLAST of Lag0031049 vs. NCBI nr
Match: XP_022998306.1 (ribosomal RNA small subunit methyltransferase [Cucurbita maxima])

HSP 1 Score: 672.5 bits (1734), Expect = 1.9e-189
Identity = 342/355 (96.34%), Postives = 348/355 (98.03%), Query Frame = 0

Query: 1   MAGGKMKREKPKAAAKSAPYQGGISFHKSKGQHILKNPLLVDSIVQKSGIKSTDVILEIG 60
           MAGGKMKREKPKA+AK+APYQGGISFHKSKGQHILKNP+LVDSIVQKSGIKSTDVILEIG
Sbjct: 1   MAGGKMKREKPKASAKTAPYQGGISFHKSKGQHILKNPMLVDSIVQKSGIKSTDVILEIG 60

Query: 61  PGTGNLTKKLLECGKMVIAVELDPRMVLELQRRFQDTPYSSRLKVIQGDVLKTELPYFDI 120
           PGTGNLTKKLLECGKMVIAVELDPRMVLELQRRFQDTPYSSRLKVIQGDVLKTELPYFDI
Sbjct: 61  PGTGNLTKKLLECGKMVIAVELDPRMVLELQRRFQDTPYSSRLKVIQGDVLKTELPYFDI 120

Query: 121 CVANIPYQISSPLTFKLLNHQPSFRCAIIMFQREFAMRLVAQPGDKLYCRLTVNTQLLAR 180
           CVANIPYQISSPLTFKLLNHQPSFRCAIIMFQREFAMRLVAQPGDKLYCRLTVNTQLL+R
Sbjct: 121 CVANIPYQISSPLTFKLLNHQPSFRCAIIMFQREFAMRLVAQPGDKLYCRLTVNTQLLSR 180

Query: 181 VSHLLKVGKNNFRPPPKVDSSVVRIEPRKPRIEVKQKEWDGFLRICFNRKNKTLGSIFRQ 240
           VSHLLKVGKNNFRPPPKVDSSVVRIEPRKPRIEVKQKEWDGFLRICFNRKNKTLGSIFRQ
Sbjct: 181 VSHLLKVGKNNFRPPPKVDSSVVRIEPRKPRIEVKQKEWDGFLRICFNRKNKTLGSIFRQ 240

Query: 241 KSVLSLLEKNYKTLCALKIPLQSSADNDDGAFDYSRFGESNEDQTMEVDDDGADEEMDMD 300
           KSVLSLLEKNYKTLCAL IP Q SAD DDG FDYSRFG SNEDQTMEVDDDGADEEMDMD
Sbjct: 241 KSVLSLLEKNYKTLCALNIPQQGSADKDDGDFDYSRFGNSNEDQTMEVDDDGADEEMDMD 300

Query: 301 DSDAEGGEGEASEFKDKVLGVLKEGDFEEKRSSKLTLQEFLYLLSLFNKAGIHFS 356
            SDAEGGEGEASEFKDKV+GVLKEGDFEEKRSSKL+LQEFLYLLSLFNKAGIHFS
Sbjct: 301 GSDAEGGEGEASEFKDKVMGVLKEGDFEEKRSSKLSLQEFLYLLSLFNKAGIHFS 355

BLAST of Lag0031049 vs. NCBI nr
Match: KAG6606941.1 (Ribosomal RNA small subunit methyltransferase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 671.8 bits (1732), Expect = 3.3e-189
Identity = 341/355 (96.06%), Postives = 347/355 (97.75%), Query Frame = 0

Query: 1   MAGGKMKREKPKAAAKSAPYQGGISFHKSKGQHILKNPLLVDSIVQKSGIKSTDVILEIG 60
           MAGGKMKREKPKA AK+APYQGGISFHKSKGQHILKNP+LVDSIVQKSGIKSTDVILEIG
Sbjct: 1   MAGGKMKREKPKALAKTAPYQGGISFHKSKGQHILKNPMLVDSIVQKSGIKSTDVILEIG 60

Query: 61  PGTGNLTKKLLECGKMVIAVELDPRMVLELQRRFQDTPYSSRLKVIQGDVLKTELPYFDI 120
           PGTGNLTKKLLECGKMVIAVELDPRMVLELQRRFQDTPYSSRLKVIQGDVLKTELPYFDI
Sbjct: 61  PGTGNLTKKLLECGKMVIAVELDPRMVLELQRRFQDTPYSSRLKVIQGDVLKTELPYFDI 120

Query: 121 CVANIPYQISSPLTFKLLNHQPSFRCAIIMFQREFAMRLVAQPGDKLYCRLTVNTQLLAR 180
           CVANIPYQISSPLTFKLLNHQPSFRCAIIMFQREFAMRLVAQPGDKLYCRLTVNTQLL+R
Sbjct: 121 CVANIPYQISSPLTFKLLNHQPSFRCAIIMFQREFAMRLVAQPGDKLYCRLTVNTQLLSR 180

Query: 181 VSHLLKVGKNNFRPPPKVDSSVVRIEPRKPRIEVKQKEWDGFLRICFNRKNKTLGSIFRQ 240
           VSHLLKVGKNNFRPPPKVDSSVVRIEPRKPRIEVKQKEWDGFLRICFNRKNKTLGSIFRQ
Sbjct: 181 VSHLLKVGKNNFRPPPKVDSSVVRIEPRKPRIEVKQKEWDGFLRICFNRKNKTLGSIFRQ 240

Query: 241 KSVLSLLEKNYKTLCALKIPLQSSADNDDGAFDYSRFGESNEDQTMEVDDDGADEEMDMD 300
           KSVLSLLEKNYKTLCAL IP Q SAD DDG FDYSRFG SNEDQTMEVDDDG DEEMDMD
Sbjct: 241 KSVLSLLEKNYKTLCALNIPQQGSADKDDGDFDYSRFGNSNEDQTMEVDDDGGDEEMDMD 300

Query: 301 DSDAEGGEGEASEFKDKVLGVLKEGDFEEKRSSKLTLQEFLYLLSLFNKAGIHFS 356
           DSDAEGG+GEASEFKDKV+GVLKEGDFEEKRSSKL+LQEFLYLLSLFNKAGIHFS
Sbjct: 301 DSDAEGGDGEASEFKDKVMGVLKEGDFEEKRSSKLSLQEFLYLLSLFNKAGIHFS 355

BLAST of Lag0031049 vs. NCBI nr
Match: KAG7036644.1 (Ribosomal RNA small subunit methyltransferase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 669.5 bits (1726), Expect = 1.6e-188
Identity = 340/355 (95.77%), Postives = 346/355 (97.46%), Query Frame = 0

Query: 1   MAGGKMKREKPKAAAKSAPYQGGISFHKSKGQHILKNPLLVDSIVQKSGIKSTDVILEIG 60
           MAGGKMKREKPKA AK+APYQGGISFHKSKGQHILKNP+LVDSIVQKSGIKSTDVILEIG
Sbjct: 1   MAGGKMKREKPKALAKTAPYQGGISFHKSKGQHILKNPMLVDSIVQKSGIKSTDVILEIG 60

Query: 61  PGTGNLTKKLLECGKMVIAVELDPRMVLELQRRFQDTPYSSRLKVIQGDVLKTELPYFDI 120
           PGTGNLTKKLLECGKMVIAVELDPRMVLELQRRFQDTPYSSRLKVIQGDVLKTELPYFDI
Sbjct: 61  PGTGNLTKKLLECGKMVIAVELDPRMVLELQRRFQDTPYSSRLKVIQGDVLKTELPYFDI 120

Query: 121 CVANIPYQISSPLTFKLLNHQPSFRCAIIMFQREFAMRLVAQPGDKLYCRLTVNTQLLAR 180
           CVANIPYQISSPLTFKLLNHQPSFRCAIIMFQREFAMRLVAQPGDKLYCRLTVNTQLL+R
Sbjct: 121 CVANIPYQISSPLTFKLLNHQPSFRCAIIMFQREFAMRLVAQPGDKLYCRLTVNTQLLSR 180

Query: 181 VSHLLKVGKNNFRPPPKVDSSVVRIEPRKPRIEVKQKEWDGFLRICFNRKNKTLGSIFRQ 240
           VSHLLKVGKNNFRPPPKVDSSVVRIEPRKPRIEVKQKEWDGFLRICFNRKNKTLGSIFRQ
Sbjct: 181 VSHLLKVGKNNFRPPPKVDSSVVRIEPRKPRIEVKQKEWDGFLRICFNRKNKTLGSIFRQ 240

Query: 241 KSVLSLLEKNYKTLCALKIPLQSSADNDDGAFDYSRFGESNEDQTMEVDDDGADEEMDMD 300
           KSVLSLLEKNYKTLCAL IP Q SAD DDG FDYSRFG  NEDQTMEVDDDG DEEMDMD
Sbjct: 241 KSVLSLLEKNYKTLCALNIPQQGSADKDDGDFDYSRFGNLNEDQTMEVDDDGGDEEMDMD 300

Query: 301 DSDAEGGEGEASEFKDKVLGVLKEGDFEEKRSSKLTLQEFLYLLSLFNKAGIHFS 356
           DSDAEGG+GEASEFKDKV+GVLKEGDFEEKRSSKL+LQEFLYLLSLFNKAGIHFS
Sbjct: 301 DSDAEGGDGEASEFKDKVMGVLKEGDFEEKRSSKLSLQEFLYLLSLFNKAGIHFS 355

BLAST of Lag0031049 vs. NCBI nr
Match: XP_022949401.1 (ribosomal RNA small subunit methyltransferase [Cucurbita moschata])

HSP 1 Score: 669.1 bits (1725), Expect = 2.1e-188
Identity = 339/355 (95.49%), Postives = 347/355 (97.75%), Query Frame = 0

Query: 1   MAGGKMKREKPKAAAKSAPYQGGISFHKSKGQHILKNPLLVDSIVQKSGIKSTDVILEIG 60
           MAGGKMKREKPKA+AK+APYQGGISFHKSKGQHILKNP+LVDSIVQKSGIKSTDVILEIG
Sbjct: 1   MAGGKMKREKPKASAKTAPYQGGISFHKSKGQHILKNPMLVDSIVQKSGIKSTDVILEIG 60

Query: 61  PGTGNLTKKLLECGKMVIAVELDPRMVLELQRRFQDTPYSSRLKVIQGDVLKTELPYFDI 120
           PGTGNLTKKLLECGKMVIAVELDPRMVLELQRRFQDTPY+SRLKVIQGDVLKTELPYFDI
Sbjct: 61  PGTGNLTKKLLECGKMVIAVELDPRMVLELQRRFQDTPYASRLKVIQGDVLKTELPYFDI 120

Query: 121 CVANIPYQISSPLTFKLLNHQPSFRCAIIMFQREFAMRLVAQPGDKLYCRLTVNTQLLAR 180
           CVANIPYQISSPLTFKLLNHQPSFRCAIIMFQREFAMRLVAQPGDKLYCRLTVNTQLL+R
Sbjct: 121 CVANIPYQISSPLTFKLLNHQPSFRCAIIMFQREFAMRLVAQPGDKLYCRLTVNTQLLSR 180

Query: 181 VSHLLKVGKNNFRPPPKVDSSVVRIEPRKPRIEVKQKEWDGFLRICFNRKNKTLGSIFRQ 240
           VSHLLKVGKNNFRPPPKVDSSVVRIEPRKPRIEVKQKEWDGFLRICFNRKNKTLGSIFRQ
Sbjct: 181 VSHLLKVGKNNFRPPPKVDSSVVRIEPRKPRIEVKQKEWDGFLRICFNRKNKTLGSIFRQ 240

Query: 241 KSVLSLLEKNYKTLCALKIPLQSSADNDDGAFDYSRFGESNEDQTMEVDDDGADEEMDMD 300
           KSVLSLLEKNYKTLCAL IP Q SAD DDG FDYSRFG  NEDQTMEVDDDG DEEMDMD
Sbjct: 241 KSVLSLLEKNYKTLCALNIPQQGSADKDDGDFDYSRFGNLNEDQTMEVDDDGGDEEMDMD 300

Query: 301 DSDAEGGEGEASEFKDKVLGVLKEGDFEEKRSSKLTLQEFLYLLSLFNKAGIHFS 356
           DSDAEGG+GEASEFKDKV+GVLKEGDFEEKRSSKL+LQEFLYLLSLFNKAGIHFS
Sbjct: 301 DSDAEGGDGEASEFKDKVMGVLKEGDFEEKRSSKLSLQEFLYLLSLFNKAGIHFS 355

BLAST of Lag0031049 vs. ExPASy Swiss-Prot
Match: O22268 (Ribosomal RNA small subunit methyltransferase OS=Arabidopsis thaliana OX=3702 GN=DIM1A PE=1 SV=1)

HSP 1 Score: 510.0 bits (1312), Expect = 2.2e-143
Identity = 272/357 (76.19%), Postives = 306/357 (85.71%), Query Frame = 0

Query: 1   MAGGKMKREKPKAA--AKSAPYQGGISFHKSKGQHILKNPLLVDSIVQKSGIKSTDVILE 60
           MAGGK+++EKPKA+  A S  YQGGISFHKSKGQHILKNPLLVDSIVQK+GIKSTDVILE
Sbjct: 1   MAGGKIRKEKPKASNRAPSNHYQGGISFHKSKGQHILKNPLLVDSIVQKAGIKSTDVILE 60

Query: 61  IGPGTGNLTKKLLECGKMVIAVELDPRMVLELQRRFQDTPYSSRLKVIQGDVLKTELPYF 120
           IGPGTGNLTKKLLE GK VIAVELD RMVLELQRRFQ TP+S+RLKVIQGDVLKTELP F
Sbjct: 61  IGPGTGNLTKKLLEAGKEVIAVELDSRMVLELQRRFQGTPFSNRLKVIQGDVLKTELPRF 120

Query: 121 DICVANIPYQISSPLTFKLLNHQPSFRCAIIMFQREFAMRLVAQPGDKLYCRLTVNTQLL 180
           DICVANIPYQISSPLTFKLL H  SFRCA+IM+QREFAMRLVAQPGD LYCRL+VNTQL 
Sbjct: 121 DICVANIPYQISSPLTFKLLFHPTSFRCAVIMYQREFAMRLVAQPGDNLYCRLSVNTQLY 180

Query: 181 ARVSHLLKVGKNNFRPPPKVDSSVVRIEPRKPRIEVKQKEWDGFLRICFNRKNKTLGSIF 240
           ARVSHLLKVGKNNFRPPPKVDSSVVRIEPR+P  +V +KEWDGFLR+CF RKNKTLGSIF
Sbjct: 181 ARVSHLLKVGKNNFRPPPKVDSSVVRIEPRRPGPQVNKKEWDGFLRVCFIRKNKTLGSIF 240

Query: 241 RQKSVLSLLEKNYKTLCALKIPLQSSADNDDGAFDYSRFGESNEDQTMEVDDDGADEEMD 300
           +QKSVLS+LEKN+KTL A+   LQ   +N + A + +     ++   ME DD+  D++ D
Sbjct: 241 KQKSVLSMLEKNFKTLQAVLASLQ---NNGEPALNTTSMDLGDQSMGMEDDDNEMDDD-D 300

Query: 301 MDDSDAEGGEGEASEFKDKVLGVLKEGDFEEKRSSKLTLQEFLYLLSLFNKAGIHFS 356
           M+  + EG  GE SEFK+KV+ VLKEG FEEKRSSKL+ QEFLYLLSLFNK+GIHF+
Sbjct: 301 MEMDEGEGDGGETSEFKEKVMNVLKEGGFEEKRSSKLSQQEFLYLLSLFNKSGIHFT 353

BLAST of Lag0031049 vs. ExPASy Swiss-Prot
Match: Q54QK7 (Probable dimethyladenosine transferase OS=Dictyostelium discoideum OX=44689 GN=dimt1 PE=3 SV=1)

HSP 1 Score: 346.7 bits (888), Expect = 3.2e-94
Identity = 184/352 (52.27%), Postives = 239/352 (67.90%), Query Frame = 0

Query: 3   GGKMKREKPKAAAKSAPYQGGISFHKSKGQHILKNPLLVDSIVQKSGIKSTDVILEIGPG 62
           G   K EK K+A  +  ++     +KS GQH+LKNPL++D+IV KS +KSTD +LEIGPG
Sbjct: 8   GVDAKVEKTKSATAARHHE--FQMNKSYGQHLLKNPLIIDAIVDKSQLKSTDTVLEIGPG 67

Query: 63  TGNLTKKLLECGKMVIAVELDPRMVLELQRRFQDTPYSSRLKVIQGDVLKTELPYFDICV 122
           TGNLT KLLE  K VIA+E+DPRM  ELQ+R   +PY+  L++I GD LK +LPYFD+CV
Sbjct: 68  TGNLTMKLLENCKKVIAIEVDPRMAAELQKRVAASPYAQHLQIILGDFLKVDLPYFDVCV 127

Query: 123 ANIPYQISSPLTFKLLNHQPSFRCAIIMFQREFAMRLVAQPGDKLYCRLTVNTQLLARVS 182
           AN+PYQISSPLTFKLL H+P FR A++MFQ+EFA+RL A+PGD LYCRL+VNTQLL++V+
Sbjct: 128 ANVPYQISSPLTFKLLAHRPIFRTAVLMFQKEFALRLGAKPGDSLYCRLSVNTQLLSKVT 187

Query: 183 HLLKVGKNNFRPPPKVDSSVVRIEPRKPRIEVKQKEWDGFLRICFNRKNKTLGSIFRQKS 242
           HL+KVGKNNF PPPKV+S+VVRIEP  P   +   EWDG +++CF+RKNKTL  IFR  S
Sbjct: 188 HLMKVGKNNFLPPPKVESAVVRIEPFNPPPPINFVEWDGLVKLCFSRKNKTLSGIFRVSS 247

Query: 243 VLSLLEKNYKTLCALKIPLQSSADNDDGAFDYSRFGESNEDQTMEVDDDGADEEMDMDDS 302
           V+  L +NYKT CAL+                   G+ N         DG+DE+M     
Sbjct: 248 VIETLNQNYKTYCALE-------------------GKMN--------TDGSDEQM----- 307

Query: 303 DAEGGEGEASEFKDKVLGVLKEGDFEEKRSSKLTLQEFLYLLSLFNKAGIHF 355
                       K+ ++  L + DF + RSSKL + +FL LL+ F++ GIHF
Sbjct: 308 ------------KELIIKTLTDNDFLDSRSSKLDINDFLKLLNKFHETGIHF 313

BLAST of Lag0031049 vs. ExPASy Swiss-Prot
Match: Q9UNQ2 (Probable dimethyladenosine transferase OS=Homo sapiens OX=9606 GN=DIMT1 PE=1 SV=1)

HSP 1 Score: 338.6 bits (867), Expect = 8.7e-92
Identity = 177/353 (50.14%), Postives = 232/353 (65.72%), Query Frame = 0

Query: 3   GGKMKREKPKAAAKSAPYQGGISFHKSKGQHILKNPLLVDSIVQKSGIKSTDVILEIGPG 62
           G +  R++ +   KSA   GG+ F+   GQHILKNPL+++SI+ K+ ++ TDV+LE+GPG
Sbjct: 10  GRRRGRQEQRRELKSA---GGLMFNTGIGQHILKNPLIINSIIDKAALRPTDVVLEVGPG 69

Query: 63  TGNLTKKLLECGKMVIAVELDPRMVLELQRRFQDTPYSSRLKVIQGDVLKTELPYFDICV 122
           TGN+T KLLE  K V+A ELDPR+V EL +R Q TP +S+L+V+ GDVLKT+LP+FD CV
Sbjct: 70  TGNMTVKLLEKAKKVVACELDPRLVAELHKRVQGTPVASKLQVLVGDVLKTDLPFFDTCV 129

Query: 123 ANIPYQISSPLTFKLLNHQPSFRCAIIMFQREFAMRLVAQPGDKLYCRLTVNTQLLARVS 182
           AN+PYQISSP  FKLL H+P FRCAI+MFQREFA+RLVA+PGDKLYCRL++NTQLLARV 
Sbjct: 130 ANLPYQISSPFVFKLLLHRPFFRCAILMFQREFALRLVAKPGDKLYCRLSINTQLLARVD 189

Query: 183 HLLKVGKNNFRPPPKVDSSVVRIEPRKPRIEVKQKEWDGFLRICFNRKNKTLGSIFRQKS 242
           HL+KVGKNNFRPPPKV+SSVVRIEP+ P   +  +EWDG +RI F RKNKTL + F+  +
Sbjct: 190 HLMKVGKNNFRPPPKVESSVVRIEPKNPPPPINFQEWDGLVRITFVRKNKTLSAAFKSSA 249

Query: 243 VLSLLEKNYKTLCALKIPLQSSADNDDGAFDYSRFGESNEDQTMEVDDDGADEEMDMDDS 302
           V  LLEKNY+  C++                            + + +D           
Sbjct: 250 VQQLLEKNYRIHCSV--------------------------HNIIIPED----------- 309

Query: 303 DAEGGEGEASEFKDKVLGVLKEGDFEEKRSSKLTLQEFLYLLSLFNKAGIHFS 356
                        DK+  +L    F +KR+  + + +F+ LL  FN  GIHFS
Sbjct: 310 ---------FSIADKIQQILTSTGFSDKRARSMDIDDFIRLLHGFNAEGIHFS 313

BLAST of Lag0031049 vs. ExPASy Swiss-Prot
Match: Q9D0D4 (Probable dimethyladenosine transferase OS=Mus musculus OX=10090 GN=Dimt1 PE=2 SV=1)

HSP 1 Score: 338.6 bits (867), Expect = 8.7e-92
Identity = 173/334 (51.80%), Postives = 223/334 (66.77%), Query Frame = 0

Query: 22  GGISFHKSKGQHILKNPLLVDSIVQKSGIKSTDVILEIGPGTGNLTKKLLECGKMVIAVE 81
           GG+ F+   GQHILKNPL+V+SI+ K+ ++ TDV+LE+GPGTGN+T KLLE  K V+A E
Sbjct: 26  GGLMFNTGIGQHILKNPLIVNSIIDKAALRPTDVVLEVGPGTGNMTVKLLEKAKKVVACE 85

Query: 82  LDPRMVLELQRRFQDTPYSSRLKVIQGDVLKTELPYFDICVANIPYQISSPLTFKLLNHQ 141
           LDPR+V EL +R Q TP +S+L+V+ GDVLK++LP+FD CVAN+PYQISSP  FKLL H+
Sbjct: 86  LDPRLVAELHKRVQGTPLASKLQVLVGDVLKSDLPFFDACVANLPYQISSPFVFKLLLHR 145

Query: 142 PSFRCAIIMFQREFAMRLVAQPGDKLYCRLTVNTQLLARVSHLLKVGKNNFRPPPKVDSS 201
           P FRCAI+MFQREFA+RLVA+PGDKLYCRL++NTQLLARV HL+KVGKNNFRPPPKV+SS
Sbjct: 146 PFFRCAILMFQREFALRLVAKPGDKLYCRLSINTQLLARVDHLMKVGKNNFRPPPKVESS 205

Query: 202 VVRIEPRKPRIEVKQKEWDGFLRICFNRKNKTLGSIFRQKSVLSLLEKNYKTLCALKIPL 261
           VVRIEP+ P   +  +EWDG +RI F RKNKTL + F+  +V  LLEKNY+  C++    
Sbjct: 206 VVRIEPKNPPPPINFQEWDGLVRITFVRKNKTLSAAFKSSAVQQLLEKNYRIHCSV---- 265

Query: 262 QSSADNDDGAFDYSRFGESNEDQTMEVDDDGADEEMDMDDSDAEGGEGEASEFKDKVLGV 321
                                 Q   + +D                        DK+  +
Sbjct: 266 ----------------------QNTVIPED--------------------FSIADKIQQI 313

Query: 322 LKEGDFEEKRSSKLTLQEFLYLLSLFNKAGIHFS 356
           L    F +KR+  + + +F+ LL  FN  GIHFS
Sbjct: 326 LTSTGFSDKRARSMDIDDFIRLLHGFNAEGIHFS 313

BLAST of Lag0031049 vs. ExPASy Swiss-Prot
Match: Q2KHT8 (Probable dimethyladenosine transferase OS=Bos taurus OX=9913 GN=DIMT1 PE=2 SV=1)

HSP 1 Score: 338.2 bits (866), Expect = 1.1e-91
Identity = 177/354 (50.00%), Postives = 233/354 (65.82%), Query Frame = 0

Query: 2   AGGKMKREKPKAAAKSAPYQGGISFHKSKGQHILKNPLLVDSIVQKSGIKSTDVILEIGP 61
           +G + +R++ +   KSA   GG+ F+   GQHILKNPL+V+SI+ K+ ++ TDV+LE+GP
Sbjct: 9   SGRRRERQQQRGQLKSA---GGLMFNTGIGQHILKNPLIVNSIIDKAALRPTDVVLEVGP 68

Query: 62  GTGNLTKKLLECGKMVIAVELDPRMVLELQRRFQDTPYSSRLKVIQGDVLKTELPYFDIC 121
           GTGN+T KLLE  K VIA ELDPR+V EL +R Q TP +S+L+V+ GDVLK +LP+FD C
Sbjct: 69  GTGNMTVKLLEKAKKVIACELDPRLVAELHKRVQGTPLASKLQVMVGDVLKADLPFFDAC 128

Query: 122 VANIPYQISSPLTFKLLNHQPSFRCAIIMFQREFAMRLVAQPGDKLYCRLTVNTQLLARV 181
           VAN+PYQISSP  FKLL H+P FRCA++MFQREFA+RLVA+PGDKLYCRL++NTQLLARV
Sbjct: 129 VANLPYQISSPFVFKLLLHRPFFRCAVLMFQREFALRLVAKPGDKLYCRLSINTQLLARV 188

Query: 182 SHLLKVGKNNFRPPPKVDSSVVRIEPRKPRIEVKQKEWDGFLRICFNRKNKTLGSIFRQK 241
            HL+KVGKNNFRPPPKV+SSVVRIEP+ P   +  +EWDG +RI F RKNKTL + F+  
Sbjct: 189 DHLMKVGKNNFRPPPKVESSVVRIEPKNPPPPINFQEWDGLVRITFVRKNKTLSAAFKSS 248

Query: 242 SVLSLLEKNYKTLCALKIPLQSSADNDDGAFDYSRFGESNEDQTMEVDDDGADEEMDMDD 301
           +V  LLEKNY+  C++                            + + +D          
Sbjct: 249 AVQQLLEKNYRIHCSV--------------------------HNIIIPED---------- 308

Query: 302 SDAEGGEGEASEFKDKVLGVLKEGDFEEKRSSKLTLQEFLYLLSLFNKAGIHFS 356
                         DK+  +L    F +KR+  + + +F+ LL  FN  GIHFS
Sbjct: 309 ----------FSIADKIQQILTSTGFSDKRARSMDIDDFIRLLHGFNAEGIHFS 313

BLAST of Lag0031049 vs. ExPASy TrEMBL
Match: A0A6J1K9W6 (rRNA adenine N(6)-methyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111492981 PE=3 SV=1)

HSP 1 Score: 672.5 bits (1734), Expect = 9.4e-190
Identity = 342/355 (96.34%), Postives = 348/355 (98.03%), Query Frame = 0

Query: 1   MAGGKMKREKPKAAAKSAPYQGGISFHKSKGQHILKNPLLVDSIVQKSGIKSTDVILEIG 60
           MAGGKMKREKPKA+AK+APYQGGISFHKSKGQHILKNP+LVDSIVQKSGIKSTDVILEIG
Sbjct: 1   MAGGKMKREKPKASAKTAPYQGGISFHKSKGQHILKNPMLVDSIVQKSGIKSTDVILEIG 60

Query: 61  PGTGNLTKKLLECGKMVIAVELDPRMVLELQRRFQDTPYSSRLKVIQGDVLKTELPYFDI 120
           PGTGNLTKKLLECGKMVIAVELDPRMVLELQRRFQDTPYSSRLKVIQGDVLKTELPYFDI
Sbjct: 61  PGTGNLTKKLLECGKMVIAVELDPRMVLELQRRFQDTPYSSRLKVIQGDVLKTELPYFDI 120

Query: 121 CVANIPYQISSPLTFKLLNHQPSFRCAIIMFQREFAMRLVAQPGDKLYCRLTVNTQLLAR 180
           CVANIPYQISSPLTFKLLNHQPSFRCAIIMFQREFAMRLVAQPGDKLYCRLTVNTQLL+R
Sbjct: 121 CVANIPYQISSPLTFKLLNHQPSFRCAIIMFQREFAMRLVAQPGDKLYCRLTVNTQLLSR 180

Query: 181 VSHLLKVGKNNFRPPPKVDSSVVRIEPRKPRIEVKQKEWDGFLRICFNRKNKTLGSIFRQ 240
           VSHLLKVGKNNFRPPPKVDSSVVRIEPRKPRIEVKQKEWDGFLRICFNRKNKTLGSIFRQ
Sbjct: 181 VSHLLKVGKNNFRPPPKVDSSVVRIEPRKPRIEVKQKEWDGFLRICFNRKNKTLGSIFRQ 240

Query: 241 KSVLSLLEKNYKTLCALKIPLQSSADNDDGAFDYSRFGESNEDQTMEVDDDGADEEMDMD 300
           KSVLSLLEKNYKTLCAL IP Q SAD DDG FDYSRFG SNEDQTMEVDDDGADEEMDMD
Sbjct: 241 KSVLSLLEKNYKTLCALNIPQQGSADKDDGDFDYSRFGNSNEDQTMEVDDDGADEEMDMD 300

Query: 301 DSDAEGGEGEASEFKDKVLGVLKEGDFEEKRSSKLTLQEFLYLLSLFNKAGIHFS 356
            SDAEGGEGEASEFKDKV+GVLKEGDFEEKRSSKL+LQEFLYLLSLFNKAGIHFS
Sbjct: 301 GSDAEGGEGEASEFKDKVMGVLKEGDFEEKRSSKLSLQEFLYLLSLFNKAGIHFS 355

BLAST of Lag0031049 vs. ExPASy TrEMBL
Match: A0A6J1DPX6 (rRNA adenine N(6)-methyltransferase OS=Momordica charantia OX=3673 GN=LOC111022457 PE=3 SV=1)

HSP 1 Score: 669.1 bits (1725), Expect = 1.0e-188
Identity = 341/355 (96.06%), Postives = 347/355 (97.75%), Query Frame = 0

Query: 1   MAGGKMKREKPKAAAKSAPYQGGISFHKSKGQHILKNPLLVDSIVQKSGIKSTDVILEIG 60
           MAGGKMKREKPKAA KS PYQGGISFHKSKGQHILKNPLLVDSIVQKSGIKSTDVILEIG
Sbjct: 1   MAGGKMKREKPKAATKSTPYQGGISFHKSKGQHILKNPLLVDSIVQKSGIKSTDVILEIG 60

Query: 61  PGTGNLTKKLLECGKMVIAVELDPRMVLELQRRFQDTPYSSRLKVIQGDVLKTELPYFDI 120
           PGTGNLTKKLLECGKMVIAVELDPRMVLELQRRFQDTPYS+RLKVIQGDVLKTELPYFDI
Sbjct: 61  PGTGNLTKKLLECGKMVIAVELDPRMVLELQRRFQDTPYSTRLKVIQGDVLKTELPYFDI 120

Query: 121 CVANIPYQISSPLTFKLLNHQPSFRCAIIMFQREFAMRLVAQPGDKLYCRLTVNTQLLAR 180
           CVANIPYQISSPLTFKLLNHQPSFRCAIIMFQREFAMRLVAQPGDKLYCRLTVNTQLLAR
Sbjct: 121 CVANIPYQISSPLTFKLLNHQPSFRCAIIMFQREFAMRLVAQPGDKLYCRLTVNTQLLAR 180

Query: 181 VSHLLKVGKNNFRPPPKVDSSVVRIEPRKPRIEVKQKEWDGFLRICFNRKNKTLGSIFRQ 240
           VSHLLKVGKNNFRPPPKVDSSVVRIEPRKPRIEVKQKEWDGFLRICFNRKNKTLGSIFRQ
Sbjct: 181 VSHLLKVGKNNFRPPPKVDSSVVRIEPRKPRIEVKQKEWDGFLRICFNRKNKTLGSIFRQ 240

Query: 241 KSVLSLLEKNYKTLCALKIPLQSSADNDDGAFDYSRFGESNEDQTMEVDDDGADEEMDMD 300
           KSVL+LLEKNYKTLCAL +P Q SA+NDDG FDYSRFG+SNEDQTMEVDDDGADEEMDMD
Sbjct: 241 KSVLTLLEKNYKTLCALNLPQQGSANNDDGDFDYSRFGDSNEDQTMEVDDDGADEEMDMD 300

Query: 301 DSDAEGGEGEASEFKDKVLGVLKEGDFEEKRSSKLTLQEFLYLLSLFNKAGIHFS 356
           DSDAE GEGEASEFK+KVL VLKEGDFEEKRSSKLTLQEFLYLLSLFNKAGIHFS
Sbjct: 301 DSDAEVGEGEASEFKNKVLSVLKEGDFEEKRSSKLTLQEFLYLLSLFNKAGIHFS 355

BLAST of Lag0031049 vs. ExPASy TrEMBL
Match: A0A6J1GBX4 (rRNA adenine N(6)-methyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111452763 PE=3 SV=1)

HSP 1 Score: 669.1 bits (1725), Expect = 1.0e-188
Identity = 339/355 (95.49%), Postives = 347/355 (97.75%), Query Frame = 0

Query: 1   MAGGKMKREKPKAAAKSAPYQGGISFHKSKGQHILKNPLLVDSIVQKSGIKSTDVILEIG 60
           MAGGKMKREKPKA+AK+APYQGGISFHKSKGQHILKNP+LVDSIVQKSGIKSTDVILEIG
Sbjct: 1   MAGGKMKREKPKASAKTAPYQGGISFHKSKGQHILKNPMLVDSIVQKSGIKSTDVILEIG 60

Query: 61  PGTGNLTKKLLECGKMVIAVELDPRMVLELQRRFQDTPYSSRLKVIQGDVLKTELPYFDI 120
           PGTGNLTKKLLECGKMVIAVELDPRMVLELQRRFQDTPY+SRLKVIQGDVLKTELPYFDI
Sbjct: 61  PGTGNLTKKLLECGKMVIAVELDPRMVLELQRRFQDTPYASRLKVIQGDVLKTELPYFDI 120

Query: 121 CVANIPYQISSPLTFKLLNHQPSFRCAIIMFQREFAMRLVAQPGDKLYCRLTVNTQLLAR 180
           CVANIPYQISSPLTFKLLNHQPSFRCAIIMFQREFAMRLVAQPGDKLYCRLTVNTQLL+R
Sbjct: 121 CVANIPYQISSPLTFKLLNHQPSFRCAIIMFQREFAMRLVAQPGDKLYCRLTVNTQLLSR 180

Query: 181 VSHLLKVGKNNFRPPPKVDSSVVRIEPRKPRIEVKQKEWDGFLRICFNRKNKTLGSIFRQ 240
           VSHLLKVGKNNFRPPPKVDSSVVRIEPRKPRIEVKQKEWDGFLRICFNRKNKTLGSIFRQ
Sbjct: 181 VSHLLKVGKNNFRPPPKVDSSVVRIEPRKPRIEVKQKEWDGFLRICFNRKNKTLGSIFRQ 240

Query: 241 KSVLSLLEKNYKTLCALKIPLQSSADNDDGAFDYSRFGESNEDQTMEVDDDGADEEMDMD 300
           KSVLSLLEKNYKTLCAL IP Q SAD DDG FDYSRFG  NEDQTMEVDDDG DEEMDMD
Sbjct: 241 KSVLSLLEKNYKTLCALNIPQQGSADKDDGDFDYSRFGNLNEDQTMEVDDDGGDEEMDMD 300

Query: 301 DSDAEGGEGEASEFKDKVLGVLKEGDFEEKRSSKLTLQEFLYLLSLFNKAGIHFS 356
           DSDAEGG+GEASEFKDKV+GVLKEGDFEEKRSSKL+LQEFLYLLSLFNKAGIHFS
Sbjct: 301 DSDAEGGDGEASEFKDKVMGVLKEGDFEEKRSSKLSLQEFLYLLSLFNKAGIHFS 355

BLAST of Lag0031049 vs. ExPASy TrEMBL
Match: A0A5A7T0N9 (rRNA adenine N(6)-methyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1228G00080 PE=3 SV=1)

HSP 1 Score: 632.9 bits (1631), Expect = 8.2e-178
Identity = 331/356 (92.98%), Postives = 339/356 (95.22%), Query Frame = 0

Query: 1   MAGGKMKREKPKAAAKSA-PYQGGISFHKSKGQHILKNPLLVDSIVQKSGIKSTDVILEI 60
           MAGGK+KREKPKAAAK+A PYQGGISFHKSKGQHILKNPLLVDSIVQKSGIKSTDVILEI
Sbjct: 1   MAGGKIKREKPKAAAKAATPYQGGISFHKSKGQHILKNPLLVDSIVQKSGIKSTDVILEI 60

Query: 61  GPGTGNLTKKLLECGKMVIAVELDPRMVLELQRRFQDTPYSSRLKVIQGDVLKTELPYFD 120
           GPGTGNLTKKLLECGKMVIAVELDPRMVLELQRRFQDTPYSSRLKVIQGDVLKTELPYFD
Sbjct: 61  GPGTGNLTKKLLECGKMVIAVELDPRMVLELQRRFQDTPYSSRLKVIQGDVLKTELPYFD 120

Query: 121 ICVANIPYQISSPLTFKLLNHQPSFRCAIIMFQREFAMRLVAQPGDKLYCRLTVNTQLLA 180
           ICVANIPYQISSPLTFKLLNHQP+FRCAIIMFQREFAMRLVAQPGDKLYCRLTVNTQLLA
Sbjct: 121 ICVANIPYQISSPLTFKLLNHQPAFRCAIIMFQREFAMRLVAQPGDKLYCRLTVNTQLLA 180

Query: 181 RVSHLLKVGKNNFRPPPKVDSSVVRIEPRKPRIEVKQKEWDGFLRICFNRKNKTLGSIFR 240
           RVSHLLKVGKNNFRPPPKVDSSVVRIEPRKPRIEVKQKEWDGFLRICFNRKNKTLGSIFR
Sbjct: 181 RVSHLLKVGKNNFRPPPKVDSSVVRIEPRKPRIEVKQKEWDGFLRICFNRKNKTLGSIFR 240

Query: 241 QKSVLSLLEKNYKTLCALKIPLQSSADNDDGAFDYSRFGESNEDQTMEVDDDGADEEMDM 300
           QKSVLSLLEKNYKTLC+L I  Q SA NDD AFDY     SNEDQ+MEVD+DG DEEMDM
Sbjct: 241 QKSVLSLLEKNYKTLCSLNILQQGSAGNDDSAFDY-----SNEDQSMEVDEDGDDEEMDM 300

Query: 301 DDSDAEGGEGEASEFKDKVLGVLKEGDFEEKRSSKLTLQEFLYLLSLFNKAGIHFS 356
           +D DAE  EGEASEFK KV+GVLKEGDFEEKRSSKLTLQEFLYLLSLFNKAGIHFS
Sbjct: 301 EDGDAE--EGEASEFKGKVMGVLKEGDFEEKRSSKLTLQEFLYLLSLFNKAGIHFS 349

BLAST of Lag0031049 vs. ExPASy TrEMBL
Match: A0A1S3B8X1 (rRNA adenine N(6)-methyltransferase OS=Cucumis melo OX=3656 GN=LOC103487290 PE=3 SV=1)

HSP 1 Score: 632.9 bits (1631), Expect = 8.2e-178
Identity = 331/356 (92.98%), Postives = 339/356 (95.22%), Query Frame = 0

Query: 1   MAGGKMKREKPKAAAKSA-PYQGGISFHKSKGQHILKNPLLVDSIVQKSGIKSTDVILEI 60
           MAGGK+KREKPKAAAK+A PYQGGISFHKSKGQHILKNPLLVDSIVQKSGIKSTDVILEI
Sbjct: 1   MAGGKIKREKPKAAAKAATPYQGGISFHKSKGQHILKNPLLVDSIVQKSGIKSTDVILEI 60

Query: 61  GPGTGNLTKKLLECGKMVIAVELDPRMVLELQRRFQDTPYSSRLKVIQGDVLKTELPYFD 120
           GPGTGNLTKKLLECGKMVIAVELDPRMVLELQRRFQDTPYSSRLKVIQGDVLKTELPYFD
Sbjct: 61  GPGTGNLTKKLLECGKMVIAVELDPRMVLELQRRFQDTPYSSRLKVIQGDVLKTELPYFD 120

Query: 121 ICVANIPYQISSPLTFKLLNHQPSFRCAIIMFQREFAMRLVAQPGDKLYCRLTVNTQLLA 180
           ICVANIPYQISSPLTFKLLNHQP+FRCAIIMFQREFAMRLVAQPGDKLYCRLTVNTQLLA
Sbjct: 121 ICVANIPYQISSPLTFKLLNHQPAFRCAIIMFQREFAMRLVAQPGDKLYCRLTVNTQLLA 180

Query: 181 RVSHLLKVGKNNFRPPPKVDSSVVRIEPRKPRIEVKQKEWDGFLRICFNRKNKTLGSIFR 240
           RVSHLLKVGKNNFRPPPKVDSSVVRIEPRKPRIEVKQKEWDGFLRICFNRKNKTLGSIFR
Sbjct: 181 RVSHLLKVGKNNFRPPPKVDSSVVRIEPRKPRIEVKQKEWDGFLRICFNRKNKTLGSIFR 240

Query: 241 QKSVLSLLEKNYKTLCALKIPLQSSADNDDGAFDYSRFGESNEDQTMEVDDDGADEEMDM 300
           QKSVLSLLEKNYKTLC+L I  Q SA NDD AFDY     SNEDQ+MEVD+DG DEEMDM
Sbjct: 241 QKSVLSLLEKNYKTLCSLNILQQGSAGNDDSAFDY-----SNEDQSMEVDEDGDDEEMDM 300

Query: 301 DDSDAEGGEGEASEFKDKVLGVLKEGDFEEKRSSKLTLQEFLYLLSLFNKAGIHFS 356
           +D DAE  EGEASEFK KV+GVLKEGDFEEKRSSKLTLQEFLYLLSLFNKAGIHFS
Sbjct: 301 EDGDAE--EGEASEFKGKVMGVLKEGDFEEKRSSKLTLQEFLYLLSLFNKAGIHFS 349

BLAST of Lag0031049 vs. TAIR 10
Match: AT2G47420.1 (Ribosomal RNA adenine dimethylase family protein )

HSP 1 Score: 510.0 bits (1312), Expect = 1.5e-144
Identity = 272/357 (76.19%), Postives = 306/357 (85.71%), Query Frame = 0

Query: 1   MAGGKMKREKPKAA--AKSAPYQGGISFHKSKGQHILKNPLLVDSIVQKSGIKSTDVILE 60
           MAGGK+++EKPKA+  A S  YQGGISFHKSKGQHILKNPLLVDSIVQK+GIKSTDVILE
Sbjct: 1   MAGGKIRKEKPKASNRAPSNHYQGGISFHKSKGQHILKNPLLVDSIVQKAGIKSTDVILE 60

Query: 61  IGPGTGNLTKKLLECGKMVIAVELDPRMVLELQRRFQDTPYSSRLKVIQGDVLKTELPYF 120
           IGPGTGNLTKKLLE GK VIAVELD RMVLELQRRFQ TP+S+RLKVIQGDVLKTELP F
Sbjct: 61  IGPGTGNLTKKLLEAGKEVIAVELDSRMVLELQRRFQGTPFSNRLKVIQGDVLKTELPRF 120

Query: 121 DICVANIPYQISSPLTFKLLNHQPSFRCAIIMFQREFAMRLVAQPGDKLYCRLTVNTQLL 180
           DICVANIPYQISSPLTFKLL H  SFRCA+IM+QREFAMRLVAQPGD LYCRL+VNTQL 
Sbjct: 121 DICVANIPYQISSPLTFKLLFHPTSFRCAVIMYQREFAMRLVAQPGDNLYCRLSVNTQLY 180

Query: 181 ARVSHLLKVGKNNFRPPPKVDSSVVRIEPRKPRIEVKQKEWDGFLRICFNRKNKTLGSIF 240
           ARVSHLLKVGKNNFRPPPKVDSSVVRIEPR+P  +V +KEWDGFLR+CF RKNKTLGSIF
Sbjct: 181 ARVSHLLKVGKNNFRPPPKVDSSVVRIEPRRPGPQVNKKEWDGFLRVCFIRKNKTLGSIF 240

Query: 241 RQKSVLSLLEKNYKTLCALKIPLQSSADNDDGAFDYSRFGESNEDQTMEVDDDGADEEMD 300
           +QKSVLS+LEKN+KTL A+   LQ   +N + A + +     ++   ME DD+  D++ D
Sbjct: 241 KQKSVLSMLEKNFKTLQAVLASLQ---NNGEPALNTTSMDLGDQSMGMEDDDNEMDDD-D 300

Query: 301 MDDSDAEGGEGEASEFKDKVLGVLKEGDFEEKRSSKLTLQEFLYLLSLFNKAGIHFS 356
           M+  + EG  GE SEFK+KV+ VLKEG FEEKRSSKL+ QEFLYLLSLFNK+GIHF+
Sbjct: 301 MEMDEGEGDGGETSEFKEKVMNVLKEGGFEEKRSSKLSQQEFLYLLSLFNKSGIHFT 353

BLAST of Lag0031049 vs. TAIR 10
Match: AT5G66360.2 (Ribosomal RNA adenine dimethylase family protein )

HSP 1 Score: 275.8 bits (704), Expect = 4.9e-74
Identity = 156/332 (46.99%), Postives = 216/332 (65.06%), Query Frame = 0

Query: 23  GISFHKSKGQHILKNPLLVDSIVQKSGIKSTDVILEIGPGTGNLTKKLLECGKMVIAVEL 82
           G+   KSKGQH+L N  ++DSIV+ S I+ TD +LEIGPGTGNLT KLLE  + V+AVEL
Sbjct: 60  GLFLCKSKGQHLLTNTRILDSIVRSSDIRPTDTVLEIGPGTGNLTMKLLEAAQNVVAVEL 119

Query: 83  DPRMVLELQRRFQDTPYSSRLKVIQGDVLKTELPYFDICVANIPYQISSPLTFKLLNHQP 142
           D RMV  L++R  D  ++ +L +IQ DVLKT+ P+FD+ VANIPY ISSPL  KL+    
Sbjct: 120 DKRMVEILRKRVSDHGFADKLTIIQKDVLKTDFPHFDLVVANIPYNISSPLVAKLVYGSN 179

Query: 143 SFRCAIIMFQREFAMRLVAQPGDKLYCRLTVNTQLLARVSHLLKVGKNNFRPPPKVDSSV 202
           +FR A ++ Q+EF+ RL+A PGD  + RL VN +L+A V  ++ V K  F PPPKVDSSV
Sbjct: 180 TFRSATLLLQKEFSRRLLANPGDSDFNRLAVNVKLVADVKFVMDVSKREFVPPPKVDSSV 239

Query: 203 VRIEPRKPRIEVKQKEWDGFLRICFNRKNKTLGSIFRQKSVLSLLEKNYKTLCALKIPLQ 262
           +RI P++   +V  +EW  F R CF +KNKTLGS+FRQK  +              + LQ
Sbjct: 240 IRITPKEIIPDVNVQEWLAFTRTCFGKKNKTLGSMFRQKKKV--------------MELQ 299

Query: 263 SSADNDDGAFDYSRFGESNEDQTMEVDDDGADEEMDMDDSDAEGGEGEASEFKDKVLGVL 322
           S +    G+ +     ++  D   +V++DG D+ + +D         +AS FK++V+ +L
Sbjct: 300 SLSAGRHGS-NVEVMNQTGGDSDSDVEEDGKDDLLCLDT--------DASMFKERVIEIL 359

Query: 323 KEGDFEEKRSSKLTLQEFLYLLSLFNKAGIHF 355
           +   FEEKR SKL+ +E L+LLSLFN+AGI F
Sbjct: 360 RTNGFEEKRPSKLSHRELLHLLSLFNQAGIFF 368

BLAST of Lag0031049 vs. TAIR 10
Match: AT5G66360.1 (Ribosomal RNA adenine dimethylase family protein )

HSP 1 Score: 231.9 bits (590), Expect = 8.1e-61
Identity = 141/332 (42.47%), Postives = 197/332 (59.34%), Query Frame = 0

Query: 23  GISFHKSKGQHILKNPLLVDSIVQKSGIKSTDVILEIGPGTGNLTKKLLECGKMVIAVEL 82
           G+   KSKGQH+L N  ++DSIV+ S I+ TD +LEIGPGTGNLT KLLE  + V+AVEL
Sbjct: 60  GLFLCKSKGQHLLTNTRILDSIVRSSDIRPTDTVLEIGPGTGNLTMKLLEAAQNVVAVEL 119

Query: 83  DPRMVLELQRRFQDTPYSSRLKVIQGDVLKTELPYFDICVANIPYQISSPLTFKLLNHQP 142
           D RMV  L++R  D  ++ +L +IQ DVLKT+ P+FD+ VANIPY ISSPL  KL+    
Sbjct: 120 DKRMVEILRKRVSDHGFADKLTIIQKDVLKTDFPHFDLVVANIPYNISSPLVAKLVYGSN 179

Query: 143 SFRCAIIMFQREFAMRLVAQPGDKLYCRLTVNTQLLARVSHLLKVGKNNFRPPPKVDSSV 202
           +FR A ++ Q+EF+ RL+A PGD  + RL VN                            
Sbjct: 180 TFRSATLLLQKEFSRRLLANPGDSDFNRLAVN---------------------------- 239

Query: 203 VRIEPRKPRIEVKQKEWDGFLRICFNRKNKTLGSIFRQKSVLSLLEKNYKTLCALKIPLQ 262
           V+I P++   +V  +EW  F R CF +KNKTLGS+FRQK  +              + LQ
Sbjct: 240 VKITPKEIIPDVNVQEWLAFTRTCFGKKNKTLGSMFRQKKKV--------------MELQ 299

Query: 263 SSADNDDGAFDYSRFGESNEDQTMEVDDDGADEEMDMDDSDAEGGEGEASEFKDKVLGVL 322
           S +    G+ +     ++  D   +V++DG D+ + +D         +AS FK++V+ +L
Sbjct: 300 SLSAGRHGS-NVEVMNQTGGDSDSDVEEDGKDDLLCLDT--------DASMFKERVIEIL 340

Query: 323 KEGDFEEKRSSKLTLQEFLYLLSLFNKAGIHF 355
           +   FEEKR SKL+ +E L+LLSLFN+AGI F
Sbjct: 360 RTNGFEEKRPSKLSHRELLHLLSLFNQAGIFF 340

BLAST of Lag0031049 vs. TAIR 10
Match: AT1G01860.1 (Ribosomal RNA adenine dimethylase family protein )

HSP 1 Score: 96.3 bits (238), Expect = 5.3e-20
Identity = 78/268 (29.10%), Postives = 122/268 (45.52%), Query Frame = 0

Query: 4   GKMKREKPKAAAKSAPYQGGISFHKSKGQHILKNPLLVDSIVQKSGIKSTDVILEIGPGT 63
           GK   +   +  KS   +G     KS GQH + N  + D +   + +K  D +LEIGPGT
Sbjct: 50  GKSSPDDYHSTLKSLNSRGRFP-RKSLGQHYMLNSDINDQLASAADVKEGDFVLEIGPGT 109

Query: 64  GNLTKKLLECGKMVIAVELDPRMVLELQRRFQDTPYSSRLKVIQGDVLKTELPYFDI--- 123
           G+LT  L+  G  V+A+E DP MV  +  RF     S + KV+Q D +K  +    +   
Sbjct: 110 GSLTNVLINLGATVLAIEKDPHMVDLVSERFAG---SDKFKVLQEDFVKCHIRSHMLSIL 169

Query: 124 --------------CVANIPYQISSPLTFKLLNHQPSFRCAIIMFQREFAMRLVAQPGDK 183
                          V+N+P+ IS+ +   LL     F   +++ Q E A+RLV +P  +
Sbjct: 170 ETRRLSHPDSALAKVVSNLPFNISTDVVKLLLPMGDIFSKVVLLLQDEAALRLV-EPALR 229

Query: 184 L--YCRLTVNTQLLARVSHLLKVGKNNFRPPPKVDSSVVRIEPRKPR---IEVKQKEWDG 243
              Y  + +     +   +  +V + NF P PKVD++VV  + + PR        K +  
Sbjct: 230 TSEYRPINILINFYSEPEYNFRVPRENFFPQPKVDAAVVTFKLKHPRDYPDVSSTKNFFS 289

Query: 244 FLRICFNRKNKTLGSIFRQKSVLSLLEK 250
            +   FN K K L    +  S    +EK
Sbjct: 290 LVNSAFNGKRKMLRKSLQHISSSPDIEK 312

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_023525787.18.7e-19096.34ribosomal RNA small subunit methyltransferase [Cucurbita pepo subsp. pepo][more]
XP_022998306.11.9e-18996.34ribosomal RNA small subunit methyltransferase [Cucurbita maxima][more]
KAG6606941.13.3e-18996.06Ribosomal RNA small subunit methyltransferase, partial [Cucurbita argyrosperma s... [more]
KAG7036644.11.6e-18895.77Ribosomal RNA small subunit methyltransferase, partial [Cucurbita argyrosperma s... [more]
XP_022949401.12.1e-18895.49ribosomal RNA small subunit methyltransferase [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
O222682.2e-14376.19Ribosomal RNA small subunit methyltransferase OS=Arabidopsis thaliana OX=3702 GN... [more]
Q54QK73.2e-9452.27Probable dimethyladenosine transferase OS=Dictyostelium discoideum OX=44689 GN=d... [more]
Q9UNQ28.7e-9250.14Probable dimethyladenosine transferase OS=Homo sapiens OX=9606 GN=DIMT1 PE=1 SV=... [more]
Q9D0D48.7e-9251.80Probable dimethyladenosine transferase OS=Mus musculus OX=10090 GN=Dimt1 PE=2 SV... [more]
Q2KHT81.1e-9150.00Probable dimethyladenosine transferase OS=Bos taurus OX=9913 GN=DIMT1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1K9W69.4e-19096.34rRNA adenine N(6)-methyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111492981 ... [more]
A0A6J1DPX61.0e-18896.06rRNA adenine N(6)-methyltransferase OS=Momordica charantia OX=3673 GN=LOC1110224... [more]
A0A6J1GBX41.0e-18895.49rRNA adenine N(6)-methyltransferase OS=Cucurbita moschata OX=3662 GN=LOC11145276... [more]
A0A5A7T0N98.2e-17892.98rRNA adenine N(6)-methyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5... [more]
A0A1S3B8X18.2e-17892.98rRNA adenine N(6)-methyltransferase OS=Cucumis melo OX=3656 GN=LOC103487290 PE=3... [more]
Match NameE-valueIdentityDescription
AT2G47420.11.5e-14476.19Ribosomal RNA adenine dimethylase family protein [more]
AT5G66360.24.9e-7446.99Ribosomal RNA adenine dimethylase family protein [more]
AT5G66360.18.1e-6142.47Ribosomal RNA adenine dimethylase family protein [more]
AT1G01860.15.3e-2029.10Ribosomal RNA adenine dimethylase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR020598Ribosomal RNA adenine methylase transferase, N-terminalSMARTSM00650rADcneu6coord: 40..209
e-value: 5.4E-87
score: 305.0
NoneNo IPR availableGENE3D3.40.50.150Vaccinia Virus protein VP39coord: 26..210
e-value: 1.3E-67
score: 229.1
NoneNo IPR availableGENE3D1.10.8.480coord: 212..355
e-value: 3.9E-37
score: 128.7
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 284..307
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 282..315
NoneNo IPR availablePANTHERPTHR11727:SF7DIMETHYLADENOSINE TRANSFERASE-RELATEDcoord: 8..349
NoneNo IPR availableCDDcd02440AdoMet_MTasescoord: 56..138
e-value: 2.63831E-9
score: 52.4323
IPR011530Ribosomal RNA adenine dimethylaseTIGRFAMTIGR00755TIGR00755coord: 25..255
e-value: 9.2E-76
score: 252.5
IPR001737Ribosomal RNA adenine methyltransferase KsgA/ErmPFAMPF00398RrnaADcoord: 25..250
e-value: 3.6E-63
score: 213.1
IPR001737Ribosomal RNA adenine methyltransferase KsgA/ErmPANTHERPTHR11727DIMETHYLADENOSINE TRANSFERASEcoord: 8..349
IPR001737Ribosomal RNA adenine methyltransferase KsgA/ErmPROSITEPS51689SAM_RNA_A_N6_MTcoord: 26..351
score: 64.782639
IPR020596Ribosomal RNA adenine methylase transferase, conserved sitePROSITEPS01131RRNA_A_DIMETHcoord: 56..83
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILY53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 21..263

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0031049.1Lag0031049.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0051301 cell division
biological_process GO:0001708 cell fate specification
biological_process GO:0031167 rRNA methylation
biological_process GO:0000154 rRNA modification
biological_process GO:0006364 rRNA processing
cellular_component GO:0005759 mitochondrial matrix
cellular_component GO:0005730 nucleolus
molecular_function GO:0003723 RNA binding
molecular_function GO:0000179 rRNA (adenine-N6,N6-)-dimethyltransferase activity
molecular_function GO:0008649 rRNA methyltransferase activity