Lag0031032 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0031032
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
Descriptionkinesin-like protein KIN-14I
Locationchr11: 3990127 .. 3992137 (-)
RNA-Seq ExpressionLag0031032
SyntenyLag0031032
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCTTTCCTTAACTATTTTTACATTAGAAATCCGCAATAGTTCTCAAAATGGACTTAGTGTACCAGATGCTAACCTTGTAAGGGTGTCGTCAACTTCGGATGTCATTAATCTAATGAACCTTGGCCAAAGGAACCGTGCCGTTGGGGCAACGGCTCTAAATGACCGTAGTAGCCGTTCCCATAGGTAAGTAATCCGTATATCTTGTGATGTTGAGAATGTGTATGGCTTCACCAGGCACCAGGCTAACTTCAATGTGAATCATTTTGTAGTTGCTTGACGGTTCATGTTCAAGGAAGAGATTTGACGTCCGGAGCCGTTCTCCGTGGCTGTATGCATCTTGTTGATTTGGCAGGAAGTGAGAGAGTTGATAAGTCTGAGGTGACTGGAGATAGACTGAAAGAGGCACAGCATATCAATAAATCTCTTTCTGCTCTAGGTGATGTGATTGCTTCTCTTGCCCAGAAGAATCCCCATGTCCCTTACAGAAACAGCAAATTGACACAACTTCTTCAAGATTCACTTGGTAAGCTGAAAGTATACATACTCTATAGCTATCGATCCACGCTCGATATCCCTCCTAGATTGACCAACAATTTGAAACGTATTTGTATCTTTTTAGGTGGGCAAGCGAAGACATTGATGTTTGTTCACATAAGCCCAGAACCTGATGCTGTTGGAGAGACATTAAGCACACTTAAATTTGCAGAGAGAGTTGCAACGGTTGAACTCGGAGCTGCTCGAGTGAACAAAGATACTTCAGATGTGAAGGAGCTCAAAGAGCAGGTATTCACATTTTGGTCTCATTTAATCTCAAGCATCTTCCTACAAGCTTTCTATTAATACTCATCAAGTGGAAACTTTACTTCATGTCAGATTGCAAGCTTAAAGGCAGCACTTGCAAGGAAAGAGGGGGCACCACAACATGTTCAACATCATGCTTCTGGGAACTCTGAGAAATTCAAGTCAAAAGCTACTACTGAGTTATCACCTTGCCAGCCTAAAAGTCAGGATGTAGATGTGTTGGTTGAACATACCATCCGCCGTCAGCCTATGGGCGATGTAGGCAACATCGAGGTATTTCTTTTCCCTATCAAATGAGGCATTTATTCAAAATAGTTAACATAGCCATTGTGATTTCATTTCATTCTGTTTGTCATTAATGAACAATAGTTAAAGGTATTAGTTCTTTCAAAAGTCTCTTTCCCCAAGTAAATTGTCCAGTTATTTGGTTGACATTACTCTAACTTGAAGTTTTACCTCAACATCAATGCTCCAGCTTCATAATAACTCTGCAATAAGACAGAAGAGGCAAAGCTTTGACATGGATGAGCTGTTGGCCAATTCACCTCCATGGCCACCCGTCAGTAGCCCTTGCCTGAACTACGGGGAGGACGATAAAGACACGGTCTCAGGCGAATGGGTTGACAAAGTAATGGTGAACAAGCAAGACGTGAACCAAGTCGAGAACCCATTAGGGTGCTGGGAGGCAGAAAATGGTCACCTAAATGATGTCTTCTATCAGAAATATCTTCAAGACTCCTCCAAACTATATACAGAACAAGGATATGGTATGTTAACTGGAGCCAACCGATTCAACATGGCTGGCATTGATGATATAGACGATCTCGACGCTGGAACAAGCGACTCATCCGAGCCAGATTTGCTCTGGCAATTTAACCACTCCAAACTTACAAGTATAGGCAACGGAATCGGATCAAAAACCAAGAAGCCGAATGGTGGAAAGCCAGTAAAGAGCCCAGAATTAAGGTAACAAAACTATACTTAATCTATGTATGCATAGTTTTAACTACAAAGTCTATCTATTGTTGTGGCTTCTTTTGATGGAGTTGTTAAATCTGTTGATCTGCAGCAAGAACTTCAGTTCTTCAATGGGCCCTTCTCCTTCACAGAAGATATCATCAAATGGAGTTGCCCTCCCACTGCACAGAAATGGGAGACAACCTGCTTCTGCTGATAACAAACGCAGAACTGGAAATAGAAAACAGTAA

mRNA sequence

ATGCTTTCCTTAACTATTTTTACATTAGAAATCCGCAATAGTTCTCAAAATGGACTTAGTGTACCAGATGCTAACCTTGTAAGGGTGTCGTCAACTTCGGATGTCATTAATCTAATGAACCTTGGCCAAAGGAACCGTGCCGTTGGGGCAACGGCTCTAAATGACCGTAGTAGCCGTTCCCATAGTTGCTTGACGGTTCATGTTCAAGGAAGAGATTTGACGTCCGGAGCCGTTCTCCGTGGCTGTATGCATCTTGTTGATTTGGCAGGAAGTGAGAGAGTTGATAAGTCTGAGGTGACTGGAGATAGACTGAAAGAGGCACAGCATATCAATAAATCTCTTTCTGCTCTAGGTGATGTGATTGCTTCTCTTGCCCAGAAGAATCCCCATGTCCCTTACAGAAACAGCAAATTGACACAACTTCTTCAAGATTCACTTGGTGGGCAAGCGAAGACATTGATGTTTGTTCACATAAGCCCAGAACCTGATGCTGTTGGAGAGACATTAAGCACACTTAAATTTGCAGAGAGAGTTGCAACGGTTGAACTCGGAGCTGCTCGAGTGAACAAAGATACTTCAGATGTGAAGGAGCTCAAAGAGCAGATTGCAAGCTTAAAGGCAGCACTTGCAAGGAAAGAGGGGGCACCACAACATGTTCAACATCATGCTTCTGGGAACTCTGAGAAATTCAAGTCAAAAGCTACTACTGAGTTATCACCTTGCCAGCCTAAAAGTCAGGATGTAGATGTGTTGGTTGAACATACCATCCGCCGTCAGCCTATGGGCGATGTAGGCAACATCGAGCTTCATAATAACTCTGCAATAAGACAGAAGAGGCAAAGCTTTGACATGGATGAGCTGTTGGCCAATTCACCTCCATGGCCACCCGTCAGTAGCCCTTGCCTGAACTACGGGGAGGACGATAAAGACACGGTCTCAGGCGAATGGGTTGACAAAGTAATGGTGAACAAGCAAGACGTGAACCAAGTCGAGAACCCATTAGGGTGCTGGGAGGCAGAAAATGGTCACCTAAATGATGTCTTCTATCAGAAATATCTTCAAGACTCCTCCAAACTATATACAGAACAAGGATATGGTATGTTAACTGGAGCCAACCGATTCAACATGGCTGGCATTGATGATATAGACGATCTCGACGCTGGAACAAGCGACTCATCCGAGCCAGATTTGCTCTGGCAATTTAACCACTCCAAACTTACAAGTATAGGCAACGGAATCGGATCAAAAACCAAGAAGCCGAATGGTGGAAAGCCAGTAAAGAGCCCAGAATTAAGCAAGAACTTCAGTTCTTCAATGGGCCCTTCTCCTTCACAGAAGATATCATCAAATGGAGTTGCCCTCCCACTGCACAGAAATGGGAGACAACCTGCTTCTGCTGATAACAAACGCAGAACTGGAAATAGAAAACAGTAA

Coding sequence (CDS)

ATGCTTTCCTTAACTATTTTTACATTAGAAATCCGCAATAGTTCTCAAAATGGACTTAGTGTACCAGATGCTAACCTTGTAAGGGTGTCGTCAACTTCGGATGTCATTAATCTAATGAACCTTGGCCAAAGGAACCGTGCCGTTGGGGCAACGGCTCTAAATGACCGTAGTAGCCGTTCCCATAGTTGCTTGACGGTTCATGTTCAAGGAAGAGATTTGACGTCCGGAGCCGTTCTCCGTGGCTGTATGCATCTTGTTGATTTGGCAGGAAGTGAGAGAGTTGATAAGTCTGAGGTGACTGGAGATAGACTGAAAGAGGCACAGCATATCAATAAATCTCTTTCTGCTCTAGGTGATGTGATTGCTTCTCTTGCCCAGAAGAATCCCCATGTCCCTTACAGAAACAGCAAATTGACACAACTTCTTCAAGATTCACTTGGTGGGCAAGCGAAGACATTGATGTTTGTTCACATAAGCCCAGAACCTGATGCTGTTGGAGAGACATTAAGCACACTTAAATTTGCAGAGAGAGTTGCAACGGTTGAACTCGGAGCTGCTCGAGTGAACAAAGATACTTCAGATGTGAAGGAGCTCAAAGAGCAGATTGCAAGCTTAAAGGCAGCACTTGCAAGGAAAGAGGGGGCACCACAACATGTTCAACATCATGCTTCTGGGAACTCTGAGAAATTCAAGTCAAAAGCTACTACTGAGTTATCACCTTGCCAGCCTAAAAGTCAGGATGTAGATGTGTTGGTTGAACATACCATCCGCCGTCAGCCTATGGGCGATGTAGGCAACATCGAGCTTCATAATAACTCTGCAATAAGACAGAAGAGGCAAAGCTTTGACATGGATGAGCTGTTGGCCAATTCACCTCCATGGCCACCCGTCAGTAGCCCTTGCCTGAACTACGGGGAGGACGATAAAGACACGGTCTCAGGCGAATGGGTTGACAAAGTAATGGTGAACAAGCAAGACGTGAACCAAGTCGAGAACCCATTAGGGTGCTGGGAGGCAGAAAATGGTCACCTAAATGATGTCTTCTATCAGAAATATCTTCAAGACTCCTCCAAACTATATACAGAACAAGGATATGGTATGTTAACTGGAGCCAACCGATTCAACATGGCTGGCATTGATGATATAGACGATCTCGACGCTGGAACAAGCGACTCATCCGAGCCAGATTTGCTCTGGCAATTTAACCACTCCAAACTTACAAGTATAGGCAACGGAATCGGATCAAAAACCAAGAAGCCGAATGGTGGAAAGCCAGTAAAGAGCCCAGAATTAAGCAAGAACTTCAGTTCTTCAATGGGCCCTTCTCCTTCACAGAAGATATCATCAAATGGAGTTGCCCTCCCACTGCACAGAAATGGGAGACAACCTGCTTCTGCTGATAACAAACGCAGAACTGGAAATAGAAAACAGTAA

Protein sequence

MLSLTIFTLEIRNSSQNGLSVPDANLVRVSSTSDVINLMNLGQRNRAVGATALNDRSSRSHSCLTVHVQGRDLTSGAVLRGCMHLVDLAGSERVDKSEVTGDRLKEAQHINKSLSALGDVIASLAQKNPHVPYRNSKLTQLLQDSLGGQAKTLMFVHISPEPDAVGETLSTLKFAERVATVELGAARVNKDTSDVKELKEQIASLKAALARKEGAPQHVQHHASGNSEKFKSKATTELSPCQPKSQDVDVLVEHTIRRQPMGDVGNIELHNNSAIRQKRQSFDMDELLANSPPWPPVSSPCLNYGEDDKDTVSGEWVDKVMVNKQDVNQVENPLGCWEAENGHLNDVFYQKYLQDSSKLYTEQGYGMLTGANRFNMAGIDDIDDLDAGTSDSSEPDLLWQFNHSKLTSIGNGIGSKTKKPNGGKPVKSPELSKNFSSSMGPSPSQKISSNGVALPLHRNGRQPASADNKRRTGNRKQ
Homology
BLAST of Lag0031032 vs. NCBI nr
Match: XP_008454311.2 (PREDICTED: kinesin-4 [Cucumis melo])

HSP 1 Score: 850.5 bits (2196), Expect = 7.0e-243
Identity = 435/469 (92.75%), Postives = 450/469 (95.95%), Query Frame = 0

Query: 9    LEIRNSSQNGLSVPDANLVRVSSTSDVINLMNLGQRNRAVGATALNDRSSRSHSCLTVHV 68
            LEIRNSSQNGLSVPDAN+V VSST D+INLMNLGQRNRAVGATALNDRSSRSHSCLTVHV
Sbjct: 555  LEIRNSSQNGLSVPDANIVSVSSTLDIINLMNLGQRNRAVGATALNDRSSRSHSCLTVHV 614

Query: 69   QGRDLTSGAVLRGCMHLVDLAGSERVDKSEVTGDRLKEAQHINKSLSALGDVIASLAQKN 128
            QGRDLTSGA+LRGCMHLVDLAGSERVDKSEVTGDRLKEAQHINKSLSALGDVIASLAQKN
Sbjct: 615  QGRDLTSGAILRGCMHLVDLAGSERVDKSEVTGDRLKEAQHINKSLSALGDVIASLAQKN 674

Query: 129  PHVPYRNSKLTQLLQDSLGGQAKTLMFVHISPEPDAVGETLSTLKFAERVATVELGAARV 188
            PHVPYRNSKLTQLLQDSLGGQAKTLMFVHISPEPDA+GETLSTLKFAERVATVELGAARV
Sbjct: 675  PHVPYRNSKLTQLLQDSLGGQAKTLMFVHISPEPDAIGETLSTLKFAERVATVELGAARV 734

Query: 189  NKDTSDVKELKEQIASLKAALARKEGAPQHVQHHASGNSEKFKSKATTELSPCQPKSQDV 248
            NKDTSDVKELKEQIASLKAALARKEGA QH    ASGNS+KFK+KA  ELSP +PKSQDV
Sbjct: 735  NKDTSDVKELKEQIASLKAALARKEGAQQHTPLPASGNSDKFKTKA-NELSPFRPKSQDV 794

Query: 249  DVLVEHTIRRQPMGDVGNIELHNNSAIRQKRQSFDMDELLANSPPWPPVSSPCLNYGEDD 308
            DVL+EHTIRRQPMGDVGNIELHNNSAIRQKRQSFDMDE+LANSPPWPPVSSPCLNY ED+
Sbjct: 795  DVLIEHTIRRQPMGDVGNIELHNNSAIRQKRQSFDMDEMLANSPPWPPVSSPCLNYREDE 854

Query: 309  KDTVSGEWVDKVMVNKQDVNQVENPLGCWEAENGHLNDVFYQKYLQDSSKLYTEQGYGML 368
            KDT SGEWVDKVMVNKQDVNQ+ENPLGCWEAENG+LND+FYQKYLQDSSKLYTEQGY ML
Sbjct: 855  KDTASGEWVDKVMVNKQDVNQIENPLGCWEAENGNLNDIFYQKYLQDSSKLYTEQGYSML 914

Query: 369  TGANRFNMAGIDDIDDLDAGTSDSSEPDLLWQFNHSKLTSIGNGIGSKTKKPNGGKPVKS 428
            TGANRFNM GIDDIDDLDAGTSDSSEPDLLWQFN SKLTSIG+GIGSKTKKPN GKPVKS
Sbjct: 915  TGANRFNMVGIDDIDDLDAGTSDSSEPDLLWQFNQSKLTSIGSGIGSKTKKPNSGKPVKS 974

Query: 429  PELSKNFSSSMGPSPSQKISSNGVALPLHRNGRQPASADNKRRTGNRKQ 478
            PELSKNF+SSMGPSPSQKI SNGVA PLHRNGRQP SADNKRRTGNRKQ
Sbjct: 975  PELSKNFNSSMGPSPSQKI-SNGVAHPLHRNGRQPTSADNKRRTGNRKQ 1021

BLAST of Lag0031032 vs. NCBI nr
Match: KAA0044397.1 (kinesin-4 [Cucumis melo var. makuwa])

HSP 1 Score: 849.0 bits (2192), Expect = 2.0e-242
Identity = 434/469 (92.54%), Postives = 450/469 (95.95%), Query Frame = 0

Query: 9    LEIRNSSQNGLSVPDANLVRVSSTSDVINLMNLGQRNRAVGATALNDRSSRSHSCLTVHV 68
            ++IRNSSQNGLSVPDANLV VSST D+INLMNLGQRNRAVGATALNDRSSRSHSCLTVHV
Sbjct: 600  IKIRNSSQNGLSVPDANLVSVSSTLDIINLMNLGQRNRAVGATALNDRSSRSHSCLTVHV 659

Query: 69   QGRDLTSGAVLRGCMHLVDLAGSERVDKSEVTGDRLKEAQHINKSLSALGDVIASLAQKN 128
            QGRDLTSGA+LRGCMHLVDLAGSERVDKSEVTGDRLKEAQHINKSLSALGDVIASLAQKN
Sbjct: 660  QGRDLTSGAILRGCMHLVDLAGSERVDKSEVTGDRLKEAQHINKSLSALGDVIASLAQKN 719

Query: 129  PHVPYRNSKLTQLLQDSLGGQAKTLMFVHISPEPDAVGETLSTLKFAERVATVELGAARV 188
            PHVPYRNSKLTQLLQDSLGGQAKTLMFVHISPEPDA+GETLSTLKFAERVATVELGAARV
Sbjct: 720  PHVPYRNSKLTQLLQDSLGGQAKTLMFVHISPEPDAIGETLSTLKFAERVATVELGAARV 779

Query: 189  NKDTSDVKELKEQIASLKAALARKEGAPQHVQHHASGNSEKFKSKATTELSPCQPKSQDV 248
            NKDTSDVKELKEQIASLKAALARKEGA QH    ASGNS+KFK+KA  ELSP +PKSQDV
Sbjct: 780  NKDTSDVKELKEQIASLKAALARKEGAQQHTPLPASGNSDKFKTKA-NELSPFRPKSQDV 839

Query: 249  DVLVEHTIRRQPMGDVGNIELHNNSAIRQKRQSFDMDELLANSPPWPPVSSPCLNYGEDD 308
            DVL+EHTIRRQPMGDVGNIELHNNSAIRQKRQSFDMDE+LANSPPWPPVSSPCLNY ED+
Sbjct: 840  DVLIEHTIRRQPMGDVGNIELHNNSAIRQKRQSFDMDEMLANSPPWPPVSSPCLNYREDE 899

Query: 309  KDTVSGEWVDKVMVNKQDVNQVENPLGCWEAENGHLNDVFYQKYLQDSSKLYTEQGYGML 368
            KDT SGEWVDKVMVNKQDVNQ+ENPLGCWEAENG+LND+FYQKYLQDSSKLYTEQGY ML
Sbjct: 900  KDTASGEWVDKVMVNKQDVNQIENPLGCWEAENGNLNDIFYQKYLQDSSKLYTEQGYSML 959

Query: 369  TGANRFNMAGIDDIDDLDAGTSDSSEPDLLWQFNHSKLTSIGNGIGSKTKKPNGGKPVKS 428
            TGANRFNM GIDDIDDLDAGTSDSSEPDLLWQFN SKLTSIG+GIGSKTKKPN GKPVKS
Sbjct: 960  TGANRFNMVGIDDIDDLDAGTSDSSEPDLLWQFNQSKLTSIGSGIGSKTKKPNSGKPVKS 1019

Query: 429  PELSKNFSSSMGPSPSQKISSNGVALPLHRNGRQPASADNKRRTGNRKQ 478
            PELSKNF+SSMGPSPSQKI SNGVA PLHRNGRQP SADNKRRTGNRKQ
Sbjct: 1020 PELSKNFNSSMGPSPSQKI-SNGVAHPLHRNGRQPTSADNKRRTGNRKQ 1066

BLAST of Lag0031032 vs. NCBI nr
Match: TYK29525.1 (kinesin-4 [Cucumis melo var. makuwa])

HSP 1 Score: 848.2 bits (2190), Expect = 3.5e-242
Identity = 433/469 (92.32%), Postives = 450/469 (95.95%), Query Frame = 0

Query: 9    LEIRNSSQNGLSVPDANLVRVSSTSDVINLMNLGQRNRAVGATALNDRSSRSHSCLTVHV 68
            ++IRNSSQNGLSVPDAN+V VSST D+INLMNLGQRNRAVGATALNDRSSRSHSCLTVHV
Sbjct: 567  IKIRNSSQNGLSVPDANIVSVSSTLDIINLMNLGQRNRAVGATALNDRSSRSHSCLTVHV 626

Query: 69   QGRDLTSGAVLRGCMHLVDLAGSERVDKSEVTGDRLKEAQHINKSLSALGDVIASLAQKN 128
            QGRDLTSGA+LRGCMHLVDLAGSERVDKSEVTGDRLKEAQHINKSLSALGDVIASLAQKN
Sbjct: 627  QGRDLTSGAILRGCMHLVDLAGSERVDKSEVTGDRLKEAQHINKSLSALGDVIASLAQKN 686

Query: 129  PHVPYRNSKLTQLLQDSLGGQAKTLMFVHISPEPDAVGETLSTLKFAERVATVELGAARV 188
            PHVPYRNSKLTQLLQDSLGGQAKTLMFVHISPEPDA+GETLSTLKFAERVATVELGAARV
Sbjct: 687  PHVPYRNSKLTQLLQDSLGGQAKTLMFVHISPEPDAIGETLSTLKFAERVATVELGAARV 746

Query: 189  NKDTSDVKELKEQIASLKAALARKEGAPQHVQHHASGNSEKFKSKATTELSPCQPKSQDV 248
            NKDTSDVKELKEQIASLKAALARKEGA QH    ASGNS+KFK+KA  ELSP +PKSQDV
Sbjct: 747  NKDTSDVKELKEQIASLKAALARKEGAQQHTPLPASGNSDKFKTKA-NELSPFRPKSQDV 806

Query: 249  DVLVEHTIRRQPMGDVGNIELHNNSAIRQKRQSFDMDELLANSPPWPPVSSPCLNYGEDD 308
            DVL+EHTIRRQPMGDVGNIELHNNSAIRQKRQSFDMDE+LANSPPWPPVSSPCLNY ED+
Sbjct: 807  DVLIEHTIRRQPMGDVGNIELHNNSAIRQKRQSFDMDEMLANSPPWPPVSSPCLNYREDE 866

Query: 309  KDTVSGEWVDKVMVNKQDVNQVENPLGCWEAENGHLNDVFYQKYLQDSSKLYTEQGYGML 368
            KDT SGEWVDKVMVNKQDVNQ+ENPLGCWEAENG+LND+FYQKYLQDSSKLYTEQGY ML
Sbjct: 867  KDTASGEWVDKVMVNKQDVNQIENPLGCWEAENGNLNDIFYQKYLQDSSKLYTEQGYSML 926

Query: 369  TGANRFNMAGIDDIDDLDAGTSDSSEPDLLWQFNHSKLTSIGNGIGSKTKKPNGGKPVKS 428
            TGANRFNM GIDDIDDLDAGTSDSSEPDLLWQFN SKLTSIG+GIGSKTKKPN GKPVKS
Sbjct: 927  TGANRFNMVGIDDIDDLDAGTSDSSEPDLLWQFNQSKLTSIGSGIGSKTKKPNSGKPVKS 986

Query: 429  PELSKNFSSSMGPSPSQKISSNGVALPLHRNGRQPASADNKRRTGNRKQ 478
            PELSKNF+SSMGPSPSQKI SNGVA PLHRNGRQP SADNKRRTGNRKQ
Sbjct: 987  PELSKNFNSSMGPSPSQKI-SNGVAHPLHRNGRQPTSADNKRRTGNRKQ 1033

BLAST of Lag0031032 vs. NCBI nr
Match: QWT43339.1 (kinesin-like protein KIN14J [Citrullus lanatus subsp. vulgaris])

HSP 1 Score: 843.2 bits (2177), Expect = 1.1e-240
Identity = 432/463 (93.30%), Postives = 444/463 (95.90%), Query Frame = 0

Query: 15   SQNGLSVPDANLVRVSSTSDVINLMNLGQRNRAVGATALNDRSSRSHSCLTVHVQGRDLT 74
            SQNGLSVPDANLV VSST D+INLMNLGQRNRAVGATALNDRSSRSHSCLTVHVQGRDLT
Sbjct: 557  SQNGLSVPDANLVSVSSTLDIINLMNLGQRNRAVGATALNDRSSRSHSCLTVHVQGRDLT 616

Query: 75   SGAVLRGCMHLVDLAGSERVDKSEVTGDRLKEAQHINKSLSALGDVIASLAQKNPHVPYR 134
            SGA+LRGCMHLVDLAGSERVDKSEVTGDRLKEAQHINKSLSALGDVIASLAQKNPHVPYR
Sbjct: 617  SGAILRGCMHLVDLAGSERVDKSEVTGDRLKEAQHINKSLSALGDVIASLAQKNPHVPYR 676

Query: 135  NSKLTQLLQDSLGGQAKTLMFVHISPEPDAVGETLSTLKFAERVATVELGAARVNKDTSD 194
            NSKLTQLLQDSLGGQAKTLMFVHISPEPDA+GETLSTLKFAERVATVELGAARVNKDTSD
Sbjct: 677  NSKLTQLLQDSLGGQAKTLMFVHISPEPDAIGETLSTLKFAERVATVELGAARVNKDTSD 736

Query: 195  VKELKEQIASLKAALARKEGAPQHVQHHASGNSEKFKSKATTELSPCQPKSQDVDVLVEH 254
            VKELKEQIASLKAALARKEGA QH    ASGNSEKFK+KA +ELSP +PKSQDVDVLVEH
Sbjct: 737  VKELKEQIASLKAALARKEGA-QHTPLPASGNSEKFKTKA-SELSPFRPKSQDVDVLVEH 796

Query: 255  TIRRQPMGDVGNIELHNNSAIRQKRQSFDMDELLANSPPWPPVSSPCLNYGEDDKDTVSG 314
            TIRRQPMGDVGNIELHNNSA+RQKRQSFDMDELLANSPPWPPVS+PCLNY EDDKDT SG
Sbjct: 797  TIRRQPMGDVGNIELHNNSAMRQKRQSFDMDELLANSPPWPPVSTPCLNYREDDKDTASG 856

Query: 315  EWVDKVMVNKQDVNQVENPLGCWEAENGHLNDVFYQKYLQDSSKLYTEQGYGMLTGANRF 374
            EWVDKVMVNKQDVNQ+ENPLGCWEAENGHLNDVFYQKYLQDSSKLYTEQGY MLTG NRF
Sbjct: 857  EWVDKVMVNKQDVNQIENPLGCWEAENGHLNDVFYQKYLQDSSKLYTEQGYNMLTGVNRF 916

Query: 375  NMAGIDDIDDLDAGTSDSSEPDLLWQFNHSKLTSIGNGIGSKTKKPNGGKPVKSPELSKN 434
            NM GIDDIDDLD GTSDSSEPDLLWQFN SKL+S+G+GIGSKTKKPNGGKP KSPELSKN
Sbjct: 917  NMVGIDDIDDLDTGTSDSSEPDLLWQFNQSKLSSLGSGIGSKTKKPNGGKPAKSPELSKN 976

Query: 435  FSSSMGPSPSQKISSNGVALPLHRNGRQPASADNKRRTGNRKQ 478
            FSSSMGPSPSQKISSNGVA PLHRNGRQP SADNKRRTGNRKQ
Sbjct: 977  FSSSMGPSPSQKISSNGVAHPLHRNGRQPNSADNKRRTGNRKQ 1017

BLAST of Lag0031032 vs. NCBI nr
Match: XP_022155292.1 (kinesin-like protein KIN-14I [Momordica charantia] >XP_022155293.1 kinesin-like protein KIN-14I [Momordica charantia] >XP_022155294.1 kinesin-like protein KIN-14I [Momordica charantia])

HSP 1 Score: 841.3 bits (2172), Expect = 4.2e-240
Identity = 435/471 (92.36%), Postives = 452/471 (95.97%), Query Frame = 0

Query: 9    LEIRNSSQNGLSVPDANLVRVSSTSDVINLMNLGQRNRAVGATALNDRSSRSHSCLTVHV 68
            LEIRNSSQNGLSVPDANLV VSSTSDVINLMNLGQRNRAVGATALNDRSSRSHSCLTVHV
Sbjct: 547  LEIRNSSQNGLSVPDANLVNVSSTSDVINLMNLGQRNRAVGATALNDRSSRSHSCLTVHV 606

Query: 69   QGRDLTSGAVLRGCMHLVDLAGSERVDKSEVTGDRLKEAQHINKSLSALGDVIASLAQKN 128
            QGRDLTSGAVLRGCMHLVDLAGSERVDKSEVTGDRLKEAQHINKSLSALGDVIASLA KN
Sbjct: 607  QGRDLTSGAVLRGCMHLVDLAGSERVDKSEVTGDRLKEAQHINKSLSALGDVIASLAHKN 666

Query: 129  PHVPYRNSKLTQLLQDSLGGQAKTLMFVHISPEPDAVGETLSTLKFAERVATVELGAARV 188
            PHVPYRNSKLTQLLQDSLGGQAKTLMFVHISPEPDA+GETLSTLKFAERVATVELGAARV
Sbjct: 667  PHVPYRNSKLTQLLQDSLGGQAKTLMFVHISPEPDAIGETLSTLKFAERVATVELGAARV 726

Query: 189  NKDTSDVKELKEQIASLKAALARKEGAPQHVQHHASGNSEKFKSKATTELSPCQPKSQDV 248
            NKDT+DVKELK+QIASLKAALARKEGAPQ +QH A GNSEKFK+KA +ELSPCQPKSQD+
Sbjct: 727  NKDTTDVKELKDQIASLKAALARKEGAPQQIQHPALGNSEKFKTKA-SELSPCQPKSQDL 786

Query: 249  DVLVEHTIRRQPMGDVGNIELHNNSAIRQKRQSFDMDELLANSPPWPPVSSPCLNYGEDD 308
            DVLVEH IRRQPMGDVGNIELHNNSA+RQKRQSFDMDELLANSPPWPP+SSPCLNYGEDD
Sbjct: 787  DVLVEHAIRRQPMGDVGNIELHNNSALRQKRQSFDMDELLANSPPWPPISSPCLNYGEDD 846

Query: 309  KDTVSGEWVDKVMVNKQDVNQVENPLGCWEAENGHLNDVFYQKYLQDSSK-LYTEQGYGM 368
            KDT SGEWVDKVMVNKQDVN+VEN LGCWE ENG+LNDVFYQKYLQDSSK +YT+Q YGM
Sbjct: 847  KDTGSGEWVDKVMVNKQDVNRVENSLGCWEVENGNLNDVFYQKYLQDSSKMMYTDQAYGM 906

Query: 369  LTGANRFNMAGIDDIDDLDAGTSDSSEPDLLWQFN-HSKLTSIGNGIGSKTKKPNGGKPV 428
            L GANRFN+ GIDDIDDLDAGTSDSSEPDLLWQFN HSKL SIGN IGSKTKKPN GKPV
Sbjct: 907  LAGANRFNVVGIDDIDDLDAGTSDSSEPDLLWQFNHHSKLASIGNSIGSKTKKPN-GKPV 966

Query: 429  KSPELSKNFSSSMGPSPSQKISSNGVALPLHRNGRQPASADNKRRTGNRKQ 478
            KSP+LSKNFSSS+GPSPSQKI SNGVALPLHRNGRQPASAD+KRRTGNRKQ
Sbjct: 967  KSPDLSKNFSSSLGPSPSQKI-SNGVALPLHRNGRQPASADSKRRTGNRKQ 1014

BLAST of Lag0031032 vs. ExPASy Swiss-Prot
Match: F4IL57 (Kinesin-like protein KIN-14I OS=Arabidopsis thaliana OX=3702 GN=KIN14I PE=2 SV=1)

HSP 1 Score: 483.8 bits (1244), Expect = 2.2e-135
Identity = 288/475 (60.63%), Postives = 337/475 (70.95%), Query Frame = 0

Query: 9   LEIRNSSQNGLSVPDANLVRVSSTSDVINLMNLGQRNRAVGATALNDRSSRSHSCLTVHV 68
           LEIRNSSQ GLSVPDA+LV VSST DVI+LM  G +NRAVG+TALNDRSSRSHSCLTVHV
Sbjct: 552 LEIRNSSQKGLSVPDASLVPVSSTFDVIDLMKTGHKNRAVGSTALNDRSSRSHSCLTVHV 611

Query: 69  QGRDLTSGAVLRGCMHLVDLAGSERVDKSEVTGDRLKEAQHINKSLSALGDVIASLAQKN 128
           QGRDLTSGAVLRGCMHLVDLAGSERVDKSEVTGDRLKEAQHIN+SLSALGDVIASLA KN
Sbjct: 612 QGRDLTSGAVLRGCMHLVDLAGSERVDKSEVTGDRLKEAQHINRSLSALGDVIASLAHKN 671

Query: 129 PHVPYRNSKLTQLLQDSLGGQAKTLMFVHISPEPDAVGETLSTLKFAERVATVELGAARV 188
           PHVPYRNSKLTQLLQDSLGGQAKTLMFVHISPE DAVGET+STLKFAERVATVELGAARV
Sbjct: 672 PHVPYRNSKLTQLLQDSLGGQAKTLMFVHISPEADAVGETISTLKFAERVATVELGAARV 731

Query: 189 NKDTSDVKELKEQIASLKAALARKEG-APQHVQHHASGNSEKFKSKATTELSPCQPKSQD 248
           N DTSDVKELKEQIA+LKAALARKE  + Q+      G SEK K+K              
Sbjct: 732 NNDTSDVKELKEQIATLKAALARKEAESQQNNILKTPGGSEKHKAK-------------- 791

Query: 249 VDVLVEHTIRRQPMGDVGNIELHNNSAIRQKRQSFDMDELLANSPPWPPVSSPCLNYGED 308
                            G +E+HNN+ + +K +S +++E+  NSPPWPPV+SP   Y ED
Sbjct: 792 ----------------TGEVEIHNNNIMTKKSESCEVEEITVNSPPWPPVASPGQAYRED 851

Query: 309 DKDTVSGEWVDKVMVN--KQDVNQVENPLGCWEAEN--GHLNDVFYQKYL-QDSSKLYTE 368
           D+   S EWVDKVMVN  + ++ +VE+  G    EN  G L + FY++ L  D+S++++E
Sbjct: 852 DRSFGSSEWVDKVMVNNRQDEMRRVESLWGGATTENGIGILPEDFYRRDLASDTSRIFSE 911

Query: 369 QGYGMLTGANRFNMAGIDDIDDLDAGTSDSSEPDLLWQFNHSKLTSIGNGIGSKTKKPNG 428
             Y +  G N       +  DDLDA TSDSSEPDLLWQFN S      + I SK KKP  
Sbjct: 912 HSYNIFMGNN-------NSTDDLDAATSDSSEPDLLWQFNQSTKIPTRSNIESKLKKP-V 971

Query: 429 GKPVKSPELSKNFSSSMG-PSPSQKISSNGVALPLHRNGRQPASADNKRRTGNRK 477
            KP++SP+   N ++++  P  SQK+  NG      R  +Q   AD KR+  N +
Sbjct: 972 SKPIRSPQSRNNSNNTVSRPLASQKV-GNG-----PRGMKQFGPADMKRKATNAR 982

BLAST of Lag0031032 vs. ExPASy Swiss-Prot
Match: Q10MN5 (Kinesin-like protein KIN-14F OS=Oryza sativa subsp. japonica OX=39947 GN=KIN14F PE=2 SV=2)

HSP 1 Score: 456.8 bits (1174), Expect = 2.9e-127
Identity = 269/475 (56.63%), Postives = 332/475 (69.89%), Query Frame = 0

Query: 9   LEIRNSSQNGLSVPDANLVRVSSTSDVINLMNLGQRNRAVGATALNDRSSRSHSCLTVHV 68
           LEIRN+SQNGL+VPDA+LV V+ST DV+ LMN+GQ+NRAVGATALNDRSSRSHSCLTVHV
Sbjct: 543 LEIRNNSQNGLNVPDASLVCVASTMDVMELMNVGQKNRAVGATALNDRSSRSHSCLTVHV 602

Query: 69  QGRDLTSGAVLRGCMHLVDLAGSERVDKSEVTGDRLKEAQHINKSLSALGDVIASLAQKN 128
           QGRDLTSG +LRGCMHLVDLAGSERVDKSEVTG+RLKEAQHINKSLSALGDVIASLAQK+
Sbjct: 603 QGRDLTSGTILRGCMHLVDLAGSERVDKSEVTGERLKEAQHINKSLSALGDVIASLAQKS 662

Query: 129 PHVPYRNSKLTQLLQDSLGGQAKTLMFVHISPEPDAVGETLSTLKFAERVATVELGAARV 188
            HVPYRNSKLTQLLQDSLGGQAKTLMFVHISPE DA+GE++STLKFAERV+TVELGAAR+
Sbjct: 663 AHVPYRNSKLTQLLQDSLGGQAKTLMFVHISPESDALGESISTLKFAERVSTVELGAARL 722

Query: 189 NKDTSDVKELKEQIASLKAALARKE-GAPQHVQHHASGNSEKFKSKATTELSPCQPKSQD 248
           NK++ +VKELKEQIA LK++LA K+ G+ Q++       + K  S   +       +   
Sbjct: 723 NKESGEVKELKEQIARLKSSLAMKDSGSEQNINRDPEAFNMKMPSPGFS-----NRRQGS 782

Query: 249 VDVLVEHTIRRQPMGDVGNIELHNNSAIRQKRQSFDMDELLA--NSPPWPPVSSPCLNYG 308
            +++   T  RQPM DVGNIE+  N  +RQK+ SFD+ +LLA  +SP WP   S      
Sbjct: 783 CELVSSQTNFRQPMEDVGNIEVRANPTLRQKKPSFDLQDLLASNDSPSWPDSISRANFQM 842

Query: 309 EDDKDTVSGEWVDKVMVNKQDVNQVENPLGCWEAENGHLNDVFYQK---------YLQDS 368
            +++ T+ GEW+DKV+VN        N +G WE ++  L D FYQ+         YL+++
Sbjct: 843 GEERVTIGGEWIDKVVVNN------NNSVGDWEGDSAALPDFFYQRYHSGTRDKQYLRNN 902

Query: 369 SKLYTEQGYGMLTGANRFNMAGIDDIDDLDAGTSDSSEPDLLWQFNHSKLTSIGNGIGSK 428
           S+   + G        RF     DD DD+D  TSDSSE D LWQFN   + S  +  GSK
Sbjct: 903 SR--KKDGNEFEQQRPRFYSTNTDDSDDIDIATSDSSESDALWQFNVQSINSSISENGSK 962

Query: 429 TKKPNGGKPVKSPELSKNFSSSMGPSPSQKISSNGVALPLHRNGRQPASADNKRR 472
            KKP     ++    ++    S  PS S+K +SNG     +R+GRQP S  + RR
Sbjct: 963 IKKPQ--TKLRESSDTRTPLHSQIPSASRK-TSNG-----NRSGRQPLSGSDSRR 996

BLAST of Lag0031032 vs. ExPASy Swiss-Prot
Match: O81635 (Kinesin-like protein KIN-14G OS=Arabidopsis thaliana OX=3702 GN=KIN14G PE=1 SV=2)

HSP 1 Score: 382.5 bits (981), Expect = 7.0e-105
Identity = 242/469 (51.60%), Postives = 306/469 (65.25%), Query Frame = 0

Query: 9   LEIRNSSQNGLSVPDANLVRVSSTSDVINLMNLGQRNRAVGATALNDRSSRSHSCLTVHV 68
           LEIRN+S NG++VP+A+LV VSST DVI LM+LG  NRAV +TA+NDRSSRSHSC+TVHV
Sbjct: 549 LEIRNNSHNGINVPEASLVPVSSTDDVIQLMDLGHMNRAVSSTAMNDRSSRSHSCVTVHV 608

Query: 69  QGRDLTSGAVLRGCMHLVDLAGSERVDKSEVTGDRLKEAQHINKSLSALGDVIASLAQKN 128
           QGRDLTSG++L G MHLVDLAGSERVDKSEVTGDRLKEAQHINKSLSALGDVI+SL+QK 
Sbjct: 609 QGRDLTSGSILHGSMHLVDLAGSERVDKSEVTGDRLKEAQHINKSLSALGDVISSLSQKT 668

Query: 129 PHVPYRNSKLTQLLQDSLGGQAKTLMFVHISPEPDAVGETLSTLKFAERVATVELGAARV 188
            HVPYRNSKLTQLLQDSLGG AKTLMFVHISPEPD +GET+STLKFAERV +VELGAARV
Sbjct: 669 SHVPYRNSKLTQLLQDSLGGSAKTLMFVHISPEPDTLGETISTLKFAERVGSVELGAARV 728

Query: 189 NKDTSDVKELKEQIASLKAALARKEGAPQHVQHHASG-NSEKFKSKATTELSPCQPKSQD 248
           NKD S+VKELKEQIA+LK AL RK G    VQ  A   N E+   + + E    +PK   
Sbjct: 729 NKDNSEVKELKEQIANLKMALVRK-GNGNDVQPTAIPINRERISRRRSLETPTIRPKLPT 788

Query: 249 VDVLVEHTIRRQPMGDVGNIELHNNSAIRQKRQSFDMDELL-ANSPPWPPVSSPCLNYGE 308
           +     ++  R  + D+   E  N+S    +R S D+ EL+ ++SP WP      LN  +
Sbjct: 789 MGNTSNNS--RPQIMDLSGPEAFNDSTASSRRHSLDIHELMKSSSPAWP---RQPLNGKD 848

Query: 309 DDKDTVSGEWVDKVMVNKQDVNQVENPLGCWEAENGHLNDVFYQKYLQDSSKLYTEQGYG 368
           +D+++ SGEW+DK     +++ Q +NP         +  + FYQ  +     LY      
Sbjct: 849 EDRESKSGEWIDK----HEELIQNQNP---------NSPEQFYQSMVPQQQSLY------ 908

Query: 369 MLTGANRFNMAGIDDIDDLDAGTSDSSEPDLLW----QFNHSKLTSIGNGIGSKTKKPNG 428
              G   F +  I D +  +A TSD S+ DLLW    Q N  K+++I N    K KK   
Sbjct: 909 --GGKQDFEVQSITDNESDEAATSDCSDSDLLWRLSVQVNVPKVSNIQNSANPKPKKIQ- 968

Query: 429 GKPVKSPELSKNFSSSMGPSPSQKISSNGVALPLHRNGRQPASADNKRR 472
            +  K  E +++   S+ P+PS++  +   + P      Q  + D KRR
Sbjct: 969 PRTAKLSE-TRSLIPSLIPAPSKRPPNTVNSQP------QRPTRDGKRR 982

BLAST of Lag0031032 vs. ExPASy Swiss-Prot
Match: Q0IMS9 (Kinesin-like protein KIN-14Q OS=Oryza sativa subsp. japonica OX=39947 GN=KIN14Q PE=1 SV=1)

HSP 1 Score: 377.9 bits (969), Expect = 1.7e-103
Identity = 239/475 (50.32%), Postives = 301/475 (63.37%), Query Frame = 0

Query: 9   LEIRNSSQNGLSVPDANLVRVSSTSDVINLMNLGQRNRAVGATALNDRSSRSHSCLTVHV 68
           LEIRN+ Q GL+VPDA++V V+ST+DV+ LMN GQ+NRAVG+TA+NDRSSRSHSCL+VHV
Sbjct: 527 LEIRNTPQKGLAVPDASIVPVTSTADVVELMNQGQKNRAVGSTAINDRSSRSHSCLSVHV 586

Query: 69  QGRDLTSGAVLRGCMHLVDLAGSERVDKSEVTGDRLKEAQHINKSLSALGDVIASLAQKN 128
           QG+ LTSGA+LRGCMHLVDLAGSERVDKSEV GDRLKEAQ+INKSLSALGDVIASLAQKN
Sbjct: 587 QGKYLTSGAMLRGCMHLVDLAGSERVDKSEVVGDRLKEAQYINKSLSALGDVIASLAQKN 646

Query: 129 PHVPYRNSKLTQLLQDSLGGQAKTLMFVHISPEPDAVGETLSTLKFAERVATVELGAARV 188
            HVPYRNSKLTQLLQDSLGGQAKTLMFVH+SPE DAVGET+STLKFAERVA+VELGAA+ 
Sbjct: 647 SHVPYRNSKLTQLLQDSLGGQAKTLMFVHVSPELDAVGETISTLKFAERVASVELGAAKA 706

Query: 189 NKDTSDVKELKEQIASLKAALARKEGAPQHVQHHASGNSEKFKSKATTELSPCQPKSQDV 248
           NK+ S+V+ELKEQIA+LKAALA+KEG P+++Q              +T+ SP   + +  
Sbjct: 707 NKEGSEVRELKEQIATLKAALAKKEGEPENIQ--------------STQSSPDMYRIKRG 766

Query: 249 DVLVEHTIRRQPMGDVGNIELHNNSAIRQKRQSFDMDELLANSPPWPPVSSPCLNYGEDD 308
           + +      RQPM +VGN+E+ NN+   QK+ SF    +L+             N   D 
Sbjct: 767 NAIPAFPKNRQPMEEVGNLEVRNNATPMQKKASFQFSGVLSE------------NNSSDL 826

Query: 309 KDTVSG-EWVDKVMVNKQDVNQVENPLGCWEAENG--HLNDVFYQKYLQDSSKLYTEQGY 368
            +  +G +  D++ V     NQ EN     E E G   L   FYQ+Y  D  +       
Sbjct: 827 AENCNGIQKTDRMAVGN---NQFENGNSILELEPGATQLPTFFYQRYDPDKQR------- 886

Query: 369 GMLTGANRFNMAGIDDIDDLDAGTSDSSEPDLLWQFNHSKLTSIGNGIGSKTKKPNGGKP 428
                  R      DD D  DA TS  S+ ++L   +  K     +GI S+     G   
Sbjct: 887 ------RRAEPVETDDSDSFDAATSSPSDQEMLLSTSGLK----ADGIASR-----GAFI 946

Query: 429 VKSPELSKNFSSSMGPSPS----QKISSNGVALPLHRNGRQPASADNKRRTGNRK 477
           +K P+ +KN   +    P+      +S   +  P+  + + P S    RRT N K
Sbjct: 947 IKKPQ-TKNTKITATKIPNLAMKSPMSEKRLQTPIRNSKQLPFSTTGGRRTRNGK 949

BLAST of Lag0031032 vs. ExPASy Swiss-Prot
Match: B9G8P1 (Kinesin-like protein KIN-14P OS=Oryza sativa subsp. japonica OX=39947 GN=KIN14P PE=3 SV=2)

HSP 1 Score: 369.4 bits (947), Expect = 6.2e-101
Identity = 231/465 (49.68%), Postives = 294/465 (63.23%), Query Frame = 0

Query: 8   TLEIRNSSQNGLSVPDANLVRVSSTSDVINLMNLGQRNRAVGATALNDRSSRSHSCLTVH 67
           T++I+NSSQ G++VPDAN+V V+STSDVI+LMNLGQ+NRAV +TA+NDRSSRSHSCLTVH
Sbjct: 575 TVDIKNSSQKGIAVPDANIVPVTSTSDVIDLMNLGQKNRAVCSTAMNDRSSRSHSCLTVH 634

Query: 68  VQGRDLTSGAVLRGCMHLVDLAGSERVDKSEVTGDRLKEAQHINKSLSALGDVIASLAQK 127
           VQGRDLTS  VLRGCMHLVDLAGSERVDKSEV GDRLKEAQHINKSL+ALGDVIASLAQK
Sbjct: 635 VQGRDLTSRTVLRGCMHLVDLAGSERVDKSEVVGDRLKEAQHINKSLAALGDVIASLAQK 694

Query: 128 NPHVPYRNSKLTQLLQDSLGGQAKTLMFVHISPEPDAVGETLSTLKFAERVATVELGAAR 187
           N HVPYRNSKLTQLLQDSLGGQAKTLMFVHI+PEPDA+GE++STLKFAERVATVELGAA+
Sbjct: 695 NAHVPYRNSKLTQLLQDSLGGQAKTLMFVHIAPEPDAIGESISTLKFAERVATVELGAAK 754

Query: 188 VNKDTSDVKELKEQIASLKAALARKEGAPQHVQHHASGNSEKFKSKATTELSPCQPKSQD 247
            NK+  +VKELKEQIA LKAALA+K+G  + ++   S                  P    
Sbjct: 755 SNKEGGEVKELKEQIACLKAALAKKDGETESIRSTQS-----------------SPDIYR 814

Query: 248 VDVLVEHTIRRQPMGDVGNIELHNNSAIRQKRQSFDMDELLANSPPWPPVSSPCLNYGED 307
           + +       R PM +VGN+E  +N   RQK+++F++ ++  ++  W   SS        
Sbjct: 815 MRMGSAPPAFRNPMEEVGNLETRSNGTPRQKKRNFELPDVENDTSSWLDTSS-------- 874

Query: 308 DKDTVSGEWVDKVMVNKQDVNQVENPLGCWEAENGHLNDVFYQKYLQDSSKLYTEQGYGM 367
            K+   GEWV+       +      P       +   + VFYQ+   +    +       
Sbjct: 875 QKEAALGEWVNNSQFGSSNSLLELGP-------DATQDVVFYQRNSPEPQWSWA------ 934

Query: 368 LTGANRFNMAGIDDIDDLDAGTSDSSEPDLLWQFNHSKLTSIGNGIGSKTKKPNGGKPVK 427
                       +D DD +  TS SSE D++   +  K     NG  S  +K    K  K
Sbjct: 935 -------GSVATEDSDDFEVTTSCSSEQDMVRPTSAPKAPGSANGSASIARKAQ-PKGAK 991

Query: 428 SPELSKNFSSSMGPSPSQKISSNGVALPLHRNGRQ--PASADNKR 471
           S ++ ++ + +   +P QK   NG      +NG+Q   ++AD KR
Sbjct: 995 STDI-RSTNPAKRAAPLQK-KINGPPSASTKNGKQLSLSAADGKR 991

BLAST of Lag0031032 vs. ExPASy TrEMBL
Match: A0A1S3BZI8 (kinesin-4 OS=Cucumis melo OX=3656 GN=LOC103494750 PE=3 SV=1)

HSP 1 Score: 850.5 bits (2196), Expect = 3.4e-243
Identity = 435/469 (92.75%), Postives = 450/469 (95.95%), Query Frame = 0

Query: 9    LEIRNSSQNGLSVPDANLVRVSSTSDVINLMNLGQRNRAVGATALNDRSSRSHSCLTVHV 68
            LEIRNSSQNGLSVPDAN+V VSST D+INLMNLGQRNRAVGATALNDRSSRSHSCLTVHV
Sbjct: 555  LEIRNSSQNGLSVPDANIVSVSSTLDIINLMNLGQRNRAVGATALNDRSSRSHSCLTVHV 614

Query: 69   QGRDLTSGAVLRGCMHLVDLAGSERVDKSEVTGDRLKEAQHINKSLSALGDVIASLAQKN 128
            QGRDLTSGA+LRGCMHLVDLAGSERVDKSEVTGDRLKEAQHINKSLSALGDVIASLAQKN
Sbjct: 615  QGRDLTSGAILRGCMHLVDLAGSERVDKSEVTGDRLKEAQHINKSLSALGDVIASLAQKN 674

Query: 129  PHVPYRNSKLTQLLQDSLGGQAKTLMFVHISPEPDAVGETLSTLKFAERVATVELGAARV 188
            PHVPYRNSKLTQLLQDSLGGQAKTLMFVHISPEPDA+GETLSTLKFAERVATVELGAARV
Sbjct: 675  PHVPYRNSKLTQLLQDSLGGQAKTLMFVHISPEPDAIGETLSTLKFAERVATVELGAARV 734

Query: 189  NKDTSDVKELKEQIASLKAALARKEGAPQHVQHHASGNSEKFKSKATTELSPCQPKSQDV 248
            NKDTSDVKELKEQIASLKAALARKEGA QH    ASGNS+KFK+KA  ELSP +PKSQDV
Sbjct: 735  NKDTSDVKELKEQIASLKAALARKEGAQQHTPLPASGNSDKFKTKA-NELSPFRPKSQDV 794

Query: 249  DVLVEHTIRRQPMGDVGNIELHNNSAIRQKRQSFDMDELLANSPPWPPVSSPCLNYGEDD 308
            DVL+EHTIRRQPMGDVGNIELHNNSAIRQKRQSFDMDE+LANSPPWPPVSSPCLNY ED+
Sbjct: 795  DVLIEHTIRRQPMGDVGNIELHNNSAIRQKRQSFDMDEMLANSPPWPPVSSPCLNYREDE 854

Query: 309  KDTVSGEWVDKVMVNKQDVNQVENPLGCWEAENGHLNDVFYQKYLQDSSKLYTEQGYGML 368
            KDT SGEWVDKVMVNKQDVNQ+ENPLGCWEAENG+LND+FYQKYLQDSSKLYTEQGY ML
Sbjct: 855  KDTASGEWVDKVMVNKQDVNQIENPLGCWEAENGNLNDIFYQKYLQDSSKLYTEQGYSML 914

Query: 369  TGANRFNMAGIDDIDDLDAGTSDSSEPDLLWQFNHSKLTSIGNGIGSKTKKPNGGKPVKS 428
            TGANRFNM GIDDIDDLDAGTSDSSEPDLLWQFN SKLTSIG+GIGSKTKKPN GKPVKS
Sbjct: 915  TGANRFNMVGIDDIDDLDAGTSDSSEPDLLWQFNQSKLTSIGSGIGSKTKKPNSGKPVKS 974

Query: 429  PELSKNFSSSMGPSPSQKISSNGVALPLHRNGRQPASADNKRRTGNRKQ 478
            PELSKNF+SSMGPSPSQKI SNGVA PLHRNGRQP SADNKRRTGNRKQ
Sbjct: 975  PELSKNFNSSMGPSPSQKI-SNGVAHPLHRNGRQPTSADNKRRTGNRKQ 1021

BLAST of Lag0031032 vs. ExPASy TrEMBL
Match: A0A5A7TRA6 (Kinesin-4 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold46G001260 PE=3 SV=1)

HSP 1 Score: 849.0 bits (2192), Expect = 9.8e-243
Identity = 434/469 (92.54%), Postives = 450/469 (95.95%), Query Frame = 0

Query: 9    LEIRNSSQNGLSVPDANLVRVSSTSDVINLMNLGQRNRAVGATALNDRSSRSHSCLTVHV 68
            ++IRNSSQNGLSVPDANLV VSST D+INLMNLGQRNRAVGATALNDRSSRSHSCLTVHV
Sbjct: 600  IKIRNSSQNGLSVPDANLVSVSSTLDIINLMNLGQRNRAVGATALNDRSSRSHSCLTVHV 659

Query: 69   QGRDLTSGAVLRGCMHLVDLAGSERVDKSEVTGDRLKEAQHINKSLSALGDVIASLAQKN 128
            QGRDLTSGA+LRGCMHLVDLAGSERVDKSEVTGDRLKEAQHINKSLSALGDVIASLAQKN
Sbjct: 660  QGRDLTSGAILRGCMHLVDLAGSERVDKSEVTGDRLKEAQHINKSLSALGDVIASLAQKN 719

Query: 129  PHVPYRNSKLTQLLQDSLGGQAKTLMFVHISPEPDAVGETLSTLKFAERVATVELGAARV 188
            PHVPYRNSKLTQLLQDSLGGQAKTLMFVHISPEPDA+GETLSTLKFAERVATVELGAARV
Sbjct: 720  PHVPYRNSKLTQLLQDSLGGQAKTLMFVHISPEPDAIGETLSTLKFAERVATVELGAARV 779

Query: 189  NKDTSDVKELKEQIASLKAALARKEGAPQHVQHHASGNSEKFKSKATTELSPCQPKSQDV 248
            NKDTSDVKELKEQIASLKAALARKEGA QH    ASGNS+KFK+KA  ELSP +PKSQDV
Sbjct: 780  NKDTSDVKELKEQIASLKAALARKEGAQQHTPLPASGNSDKFKTKA-NELSPFRPKSQDV 839

Query: 249  DVLVEHTIRRQPMGDVGNIELHNNSAIRQKRQSFDMDELLANSPPWPPVSSPCLNYGEDD 308
            DVL+EHTIRRQPMGDVGNIELHNNSAIRQKRQSFDMDE+LANSPPWPPVSSPCLNY ED+
Sbjct: 840  DVLIEHTIRRQPMGDVGNIELHNNSAIRQKRQSFDMDEMLANSPPWPPVSSPCLNYREDE 899

Query: 309  KDTVSGEWVDKVMVNKQDVNQVENPLGCWEAENGHLNDVFYQKYLQDSSKLYTEQGYGML 368
            KDT SGEWVDKVMVNKQDVNQ+ENPLGCWEAENG+LND+FYQKYLQDSSKLYTEQGY ML
Sbjct: 900  KDTASGEWVDKVMVNKQDVNQIENPLGCWEAENGNLNDIFYQKYLQDSSKLYTEQGYSML 959

Query: 369  TGANRFNMAGIDDIDDLDAGTSDSSEPDLLWQFNHSKLTSIGNGIGSKTKKPNGGKPVKS 428
            TGANRFNM GIDDIDDLDAGTSDSSEPDLLWQFN SKLTSIG+GIGSKTKKPN GKPVKS
Sbjct: 960  TGANRFNMVGIDDIDDLDAGTSDSSEPDLLWQFNQSKLTSIGSGIGSKTKKPNSGKPVKS 1019

Query: 429  PELSKNFSSSMGPSPSQKISSNGVALPLHRNGRQPASADNKRRTGNRKQ 478
            PELSKNF+SSMGPSPSQKI SNGVA PLHRNGRQP SADNKRRTGNRKQ
Sbjct: 1020 PELSKNFNSSMGPSPSQKI-SNGVAHPLHRNGRQPTSADNKRRTGNRKQ 1066

BLAST of Lag0031032 vs. ExPASy TrEMBL
Match: A0A5D3E2I5 (Kinesin-4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold655G001280 PE=3 SV=1)

HSP 1 Score: 848.2 bits (2190), Expect = 1.7e-242
Identity = 433/469 (92.32%), Postives = 450/469 (95.95%), Query Frame = 0

Query: 9    LEIRNSSQNGLSVPDANLVRVSSTSDVINLMNLGQRNRAVGATALNDRSSRSHSCLTVHV 68
            ++IRNSSQNGLSVPDAN+V VSST D+INLMNLGQRNRAVGATALNDRSSRSHSCLTVHV
Sbjct: 567  IKIRNSSQNGLSVPDANIVSVSSTLDIINLMNLGQRNRAVGATALNDRSSRSHSCLTVHV 626

Query: 69   QGRDLTSGAVLRGCMHLVDLAGSERVDKSEVTGDRLKEAQHINKSLSALGDVIASLAQKN 128
            QGRDLTSGA+LRGCMHLVDLAGSERVDKSEVTGDRLKEAQHINKSLSALGDVIASLAQKN
Sbjct: 627  QGRDLTSGAILRGCMHLVDLAGSERVDKSEVTGDRLKEAQHINKSLSALGDVIASLAQKN 686

Query: 129  PHVPYRNSKLTQLLQDSLGGQAKTLMFVHISPEPDAVGETLSTLKFAERVATVELGAARV 188
            PHVPYRNSKLTQLLQDSLGGQAKTLMFVHISPEPDA+GETLSTLKFAERVATVELGAARV
Sbjct: 687  PHVPYRNSKLTQLLQDSLGGQAKTLMFVHISPEPDAIGETLSTLKFAERVATVELGAARV 746

Query: 189  NKDTSDVKELKEQIASLKAALARKEGAPQHVQHHASGNSEKFKSKATTELSPCQPKSQDV 248
            NKDTSDVKELKEQIASLKAALARKEGA QH    ASGNS+KFK+KA  ELSP +PKSQDV
Sbjct: 747  NKDTSDVKELKEQIASLKAALARKEGAQQHTPLPASGNSDKFKTKA-NELSPFRPKSQDV 806

Query: 249  DVLVEHTIRRQPMGDVGNIELHNNSAIRQKRQSFDMDELLANSPPWPPVSSPCLNYGEDD 308
            DVL+EHTIRRQPMGDVGNIELHNNSAIRQKRQSFDMDE+LANSPPWPPVSSPCLNY ED+
Sbjct: 807  DVLIEHTIRRQPMGDVGNIELHNNSAIRQKRQSFDMDEMLANSPPWPPVSSPCLNYREDE 866

Query: 309  KDTVSGEWVDKVMVNKQDVNQVENPLGCWEAENGHLNDVFYQKYLQDSSKLYTEQGYGML 368
            KDT SGEWVDKVMVNKQDVNQ+ENPLGCWEAENG+LND+FYQKYLQDSSKLYTEQGY ML
Sbjct: 867  KDTASGEWVDKVMVNKQDVNQIENPLGCWEAENGNLNDIFYQKYLQDSSKLYTEQGYSML 926

Query: 369  TGANRFNMAGIDDIDDLDAGTSDSSEPDLLWQFNHSKLTSIGNGIGSKTKKPNGGKPVKS 428
            TGANRFNM GIDDIDDLDAGTSDSSEPDLLWQFN SKLTSIG+GIGSKTKKPN GKPVKS
Sbjct: 927  TGANRFNMVGIDDIDDLDAGTSDSSEPDLLWQFNQSKLTSIGSGIGSKTKKPNSGKPVKS 986

Query: 429  PELSKNFSSSMGPSPSQKISSNGVALPLHRNGRQPASADNKRRTGNRKQ 478
            PELSKNF+SSMGPSPSQKI SNGVA PLHRNGRQP SADNKRRTGNRKQ
Sbjct: 987  PELSKNFNSSMGPSPSQKI-SNGVAHPLHRNGRQPTSADNKRRTGNRKQ 1033

BLAST of Lag0031032 vs. ExPASy TrEMBL
Match: A0A6J1DMK6 (kinesin-like protein KIN-14I OS=Momordica charantia OX=3673 GN=LOC111022430 PE=3 SV=1)

HSP 1 Score: 841.3 bits (2172), Expect = 2.0e-240
Identity = 435/471 (92.36%), Postives = 452/471 (95.97%), Query Frame = 0

Query: 9    LEIRNSSQNGLSVPDANLVRVSSTSDVINLMNLGQRNRAVGATALNDRSSRSHSCLTVHV 68
            LEIRNSSQNGLSVPDANLV VSSTSDVINLMNLGQRNRAVGATALNDRSSRSHSCLTVHV
Sbjct: 547  LEIRNSSQNGLSVPDANLVNVSSTSDVINLMNLGQRNRAVGATALNDRSSRSHSCLTVHV 606

Query: 69   QGRDLTSGAVLRGCMHLVDLAGSERVDKSEVTGDRLKEAQHINKSLSALGDVIASLAQKN 128
            QGRDLTSGAVLRGCMHLVDLAGSERVDKSEVTGDRLKEAQHINKSLSALGDVIASLA KN
Sbjct: 607  QGRDLTSGAVLRGCMHLVDLAGSERVDKSEVTGDRLKEAQHINKSLSALGDVIASLAHKN 666

Query: 129  PHVPYRNSKLTQLLQDSLGGQAKTLMFVHISPEPDAVGETLSTLKFAERVATVELGAARV 188
            PHVPYRNSKLTQLLQDSLGGQAKTLMFVHISPEPDA+GETLSTLKFAERVATVELGAARV
Sbjct: 667  PHVPYRNSKLTQLLQDSLGGQAKTLMFVHISPEPDAIGETLSTLKFAERVATVELGAARV 726

Query: 189  NKDTSDVKELKEQIASLKAALARKEGAPQHVQHHASGNSEKFKSKATTELSPCQPKSQDV 248
            NKDT+DVKELK+QIASLKAALARKEGAPQ +QH A GNSEKFK+KA +ELSPCQPKSQD+
Sbjct: 727  NKDTTDVKELKDQIASLKAALARKEGAPQQIQHPALGNSEKFKTKA-SELSPCQPKSQDL 786

Query: 249  DVLVEHTIRRQPMGDVGNIELHNNSAIRQKRQSFDMDELLANSPPWPPVSSPCLNYGEDD 308
            DVLVEH IRRQPMGDVGNIELHNNSA+RQKRQSFDMDELLANSPPWPP+SSPCLNYGEDD
Sbjct: 787  DVLVEHAIRRQPMGDVGNIELHNNSALRQKRQSFDMDELLANSPPWPPISSPCLNYGEDD 846

Query: 309  KDTVSGEWVDKVMVNKQDVNQVENPLGCWEAENGHLNDVFYQKYLQDSSK-LYTEQGYGM 368
            KDT SGEWVDKVMVNKQDVN+VEN LGCWE ENG+LNDVFYQKYLQDSSK +YT+Q YGM
Sbjct: 847  KDTGSGEWVDKVMVNKQDVNRVENSLGCWEVENGNLNDVFYQKYLQDSSKMMYTDQAYGM 906

Query: 369  LTGANRFNMAGIDDIDDLDAGTSDSSEPDLLWQFN-HSKLTSIGNGIGSKTKKPNGGKPV 428
            L GANRFN+ GIDDIDDLDAGTSDSSEPDLLWQFN HSKL SIGN IGSKTKKPN GKPV
Sbjct: 907  LAGANRFNVVGIDDIDDLDAGTSDSSEPDLLWQFNHHSKLASIGNSIGSKTKKPN-GKPV 966

Query: 429  KSPELSKNFSSSMGPSPSQKISSNGVALPLHRNGRQPASADNKRRTGNRKQ 478
            KSP+LSKNFSSS+GPSPSQKI SNGVALPLHRNGRQPASAD+KRRTGNRKQ
Sbjct: 967  KSPDLSKNFSSSLGPSPSQKI-SNGVALPLHRNGRQPASADSKRRTGNRKQ 1014

BLAST of Lag0031032 vs. ExPASy TrEMBL
Match: A0A6J1F5B9 (kinesin-like protein KIN-14I isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111442299 PE=3 SV=1)

HSP 1 Score: 830.5 bits (2144), Expect = 3.6e-237
Identity = 428/471 (90.87%), Postives = 448/471 (95.12%), Query Frame = 0

Query: 9    LEIRNSSQNGLSVPDANLVRVSSTSDVINLMNLGQRNRAVGATALNDRSSRSHSCLTVHV 68
            LEIRNSSQNGLSVPDANLV VSST D+INLMNLG RNR VGATALNDRSSRSHSCLTVHV
Sbjct: 554  LEIRNSSQNGLSVPDANLVSVSSTLDIINLMNLGHRNRVVGATALNDRSSRSHSCLTVHV 613

Query: 69   QGRDLTSGAVLRGCMHLVDLAGSERVDKSEVTGDRLKEAQHINKSLSALGDVIASLAQKN 128
            QGRDLTSGA+LRGCMHLVDLAGSERVDKSEVTGDRLKEAQHINKSLSALGDVIASLAQKN
Sbjct: 614  QGRDLTSGAILRGCMHLVDLAGSERVDKSEVTGDRLKEAQHINKSLSALGDVIASLAQKN 673

Query: 129  PHVPYRNSKLTQLLQDSLGGQAKTLMFVHISPEPDAVGETLSTLKFAERVATVELGAARV 188
            PHVPYRNSKLTQLLQDSLGGQAKTLMFVHISPEPDA+GETLSTLKFAERVATVELGAARV
Sbjct: 674  PHVPYRNSKLTQLLQDSLGGQAKTLMFVHISPEPDAIGETLSTLKFAERVATVELGAARV 733

Query: 189  NKDTSDVKELKEQIASLKAALARKEGA-PQHVQHHASGNSEKFKSKATTELSPCQPKSQD 248
            NKDTSDVKELKEQIASLKAALARKEGA  QH QH ASGN EKFK+KA +E+SP +PKSQD
Sbjct: 734  NKDTSDVKELKEQIASLKAALARKEGAQQQHAQHSASGN-EKFKTKA-SEISPFRPKSQD 793

Query: 249  VDVLVEHTIRRQPMGDVGNIELHNNSAIRQKRQSFDMDELLANSPPWPPVSSPCLNYGED 308
            VDVLVEH IRRQPMGDVGNIELHNNSA+RQKRQSFDMDELLANSPPWPPVSSPCLNY ED
Sbjct: 794  VDVLVEHAIRRQPMGDVGNIELHNNSALRQKRQSFDMDELLANSPPWPPVSSPCLNYRED 853

Query: 309  DKDTVSGEWVDKVMVNKQDVNQVENPL-GCWEAENGHLNDVFYQKYLQDSSKLYTEQGYG 368
            DKDT SGEWVDKVMVNKQDVN++ENPL GCWE ENGHLNDVF+QKYLQDSSKLYTEQG+ 
Sbjct: 854  DKDTASGEWVDKVMVNKQDVNRIENPLGGCWETENGHLNDVFHQKYLQDSSKLYTEQGFS 913

Query: 369  MLTGANRFNMAGIDDIDDLDAGTSDSSEPDLLWQFNHSKLTSIGNGIGSKTKKPNGGKPV 428
            MLTGANRFN+ GIDDIDD+DAGTSDSSEPDLLWQFNHSKLTSIG+GIGSKTKKPNGGK V
Sbjct: 914  MLTGANRFNLVGIDDIDDIDAGTSDSSEPDLLWQFNHSKLTSIGSGIGSKTKKPNGGKAV 973

Query: 429  KSPELSKNFSSSMGPSPSQKISSNGVALPLHRNGRQPASADNKRRTGNRKQ 478
            KSPELS+N SSSMGPSPSQKIS+   ALP++RNGRQPASA+NKRRTGNRKQ
Sbjct: 974  KSPELSRNLSSSMGPSPSQKISNGVGALPMNRNGRQPASAENKRRTGNRKQ 1022

BLAST of Lag0031032 vs. TAIR 10
Match: AT2G47500.1 (P-loop nucleoside triphosphate hydrolases superfamily protein with CH (Calponin Homology) domain )

HSP 1 Score: 483.8 bits (1244), Expect = 1.6e-136
Identity = 288/475 (60.63%), Postives = 337/475 (70.95%), Query Frame = 0

Query: 9   LEIRNSSQNGLSVPDANLVRVSSTSDVINLMNLGQRNRAVGATALNDRSSRSHSCLTVHV 68
           LEIRNSSQ GLSVPDA+LV VSST DVI+LM  G +NRAVG+TALNDRSSRSHSCLTVHV
Sbjct: 552 LEIRNSSQKGLSVPDASLVPVSSTFDVIDLMKTGHKNRAVGSTALNDRSSRSHSCLTVHV 611

Query: 69  QGRDLTSGAVLRGCMHLVDLAGSERVDKSEVTGDRLKEAQHINKSLSALGDVIASLAQKN 128
           QGRDLTSGAVLRGCMHLVDLAGSERVDKSEVTGDRLKEAQHIN+SLSALGDVIASLA KN
Sbjct: 612 QGRDLTSGAVLRGCMHLVDLAGSERVDKSEVTGDRLKEAQHINRSLSALGDVIASLAHKN 671

Query: 129 PHVPYRNSKLTQLLQDSLGGQAKTLMFVHISPEPDAVGETLSTLKFAERVATVELGAARV 188
           PHVPYRNSKLTQLLQDSLGGQAKTLMFVHISPE DAVGET+STLKFAERVATVELGAARV
Sbjct: 672 PHVPYRNSKLTQLLQDSLGGQAKTLMFVHISPEADAVGETISTLKFAERVATVELGAARV 731

Query: 189 NKDTSDVKELKEQIASLKAALARKEG-APQHVQHHASGNSEKFKSKATTELSPCQPKSQD 248
           N DTSDVKELKEQIA+LKAALARKE  + Q+      G SEK K+K              
Sbjct: 732 NNDTSDVKELKEQIATLKAALARKEAESQQNNILKTPGGSEKHKAK-------------- 791

Query: 249 VDVLVEHTIRRQPMGDVGNIELHNNSAIRQKRQSFDMDELLANSPPWPPVSSPCLNYGED 308
                            G +E+HNN+ + +K +S +++E+  NSPPWPPV+SP   Y ED
Sbjct: 792 ----------------TGEVEIHNNNIMTKKSESCEVEEITVNSPPWPPVASPGQAYRED 851

Query: 309 DKDTVSGEWVDKVMVN--KQDVNQVENPLGCWEAEN--GHLNDVFYQKYL-QDSSKLYTE 368
           D+   S EWVDKVMVN  + ++ +VE+  G    EN  G L + FY++ L  D+S++++E
Sbjct: 852 DRSFGSSEWVDKVMVNNRQDEMRRVESLWGGATTENGIGILPEDFYRRDLASDTSRIFSE 911

Query: 369 QGYGMLTGANRFNMAGIDDIDDLDAGTSDSSEPDLLWQFNHSKLTSIGNGIGSKTKKPNG 428
             Y +  G N       +  DDLDA TSDSSEPDLLWQFN S      + I SK KKP  
Sbjct: 912 HSYNIFMGNN-------NSTDDLDAATSDSSEPDLLWQFNQSTKIPTRSNIESKLKKP-V 971

Query: 429 GKPVKSPELSKNFSSSMG-PSPSQKISSNGVALPLHRNGRQPASADNKRRTGNRK 477
            KP++SP+   N ++++  P  SQK+  NG      R  +Q   AD KR+  N +
Sbjct: 972 SKPIRSPQSRNNSNNTVSRPLASQKV-GNG-----PRGMKQFGPADMKRKATNAR 982

BLAST of Lag0031032 vs. TAIR 10
Match: AT5G27000.1 (kinesin 4 )

HSP 1 Score: 382.5 bits (981), Expect = 5.0e-106
Identity = 242/469 (51.60%), Postives = 306/469 (65.25%), Query Frame = 0

Query: 9   LEIRNSSQNGLSVPDANLVRVSSTSDVINLMNLGQRNRAVGATALNDRSSRSHSCLTVHV 68
           LEIRN+S NG++VP+A+LV VSST DVI LM+LG  NRAV +TA+NDRSSRSHSC+TVHV
Sbjct: 549 LEIRNNSHNGINVPEASLVPVSSTDDVIQLMDLGHMNRAVSSTAMNDRSSRSHSCVTVHV 608

Query: 69  QGRDLTSGAVLRGCMHLVDLAGSERVDKSEVTGDRLKEAQHINKSLSALGDVIASLAQKN 128
           QGRDLTSG++L G MHLVDLAGSERVDKSEVTGDRLKEAQHINKSLSALGDVI+SL+QK 
Sbjct: 609 QGRDLTSGSILHGSMHLVDLAGSERVDKSEVTGDRLKEAQHINKSLSALGDVISSLSQKT 668

Query: 129 PHVPYRNSKLTQLLQDSLGGQAKTLMFVHISPEPDAVGETLSTLKFAERVATVELGAARV 188
            HVPYRNSKLTQLLQDSLGG AKTLMFVHISPEPD +GET+STLKFAERV +VELGAARV
Sbjct: 669 SHVPYRNSKLTQLLQDSLGGSAKTLMFVHISPEPDTLGETISTLKFAERVGSVELGAARV 728

Query: 189 NKDTSDVKELKEQIASLKAALARKEGAPQHVQHHASG-NSEKFKSKATTELSPCQPKSQD 248
           NKD S+VKELKEQIA+LK AL RK G    VQ  A   N E+   + + E    +PK   
Sbjct: 729 NKDNSEVKELKEQIANLKMALVRK-GNGNDVQPTAIPINRERISRRRSLETPTIRPKLPT 788

Query: 249 VDVLVEHTIRRQPMGDVGNIELHNNSAIRQKRQSFDMDELL-ANSPPWPPVSSPCLNYGE 308
           +     ++  R  + D+   E  N+S    +R S D+ EL+ ++SP WP      LN  +
Sbjct: 789 MGNTSNNS--RPQIMDLSGPEAFNDSTASSRRHSLDIHELMKSSSPAWP---RQPLNGKD 848

Query: 309 DDKDTVSGEWVDKVMVNKQDVNQVENPLGCWEAENGHLNDVFYQKYLQDSSKLYTEQGYG 368
           +D+++ SGEW+DK     +++ Q +NP         +  + FYQ  +     LY      
Sbjct: 849 EDRESKSGEWIDK----HEELIQNQNP---------NSPEQFYQSMVPQQQSLY------ 908

Query: 369 MLTGANRFNMAGIDDIDDLDAGTSDSSEPDLLW----QFNHSKLTSIGNGIGSKTKKPNG 428
              G   F +  I D +  +A TSD S+ DLLW    Q N  K+++I N    K KK   
Sbjct: 909 --GGKQDFEVQSITDNESDEAATSDCSDSDLLWRLSVQVNVPKVSNIQNSANPKPKKIQ- 968

Query: 429 GKPVKSPELSKNFSSSMGPSPSQKISSNGVALPLHRNGRQPASADNKRR 472
            +  K  E +++   S+ P+PS++  +   + P      Q  + D KRR
Sbjct: 969 PRTAKLSE-TRSLIPSLIPAPSKRPPNTVNSQP------QRPTRDGKRR 982

BLAST of Lag0031032 vs. TAIR 10
Match: AT1G09170.1 (P-loop nucleoside triphosphate hydrolases superfamily protein with CH (Calponin Homology) domain )

HSP 1 Score: 358.2 bits (918), Expect = 1.0e-98
Identity = 225/445 (50.56%), Postives = 291/445 (65.39%), Query Frame = 0

Query: 10  EIRNSSQNGLSVPDANLVRVSSTSDVINLMNLGQRNRAVGATALNDRSSRSHSCLTVHVQ 69
           +IRNS+Q+G++VP+A LV VS+TSDVI+LMN+GQ+NRAV ATA+NDRSSRSHSCLTVHVQ
Sbjct: 553 KIRNSTQDGINVPEATLVPVSTTSDVIHLMNIGQKNRAVSATAMNDRSSRSHSCLTVHVQ 612

Query: 70  GRDLTSGAVLRGCMHLVDLAGSERVDKSEVTGDRLKEAQHINKSLSALGDVIASLAQKNP 129
           G+DLTSG  LRG MHLVDLAGSER+DKSEVTGDRLKEAQHINKSLSALGDVIASL+QKN 
Sbjct: 613 GKDLTSGVTLRGSMHLVDLAGSERIDKSEVTGDRLKEAQHINKSLSALGDVIASLSQKNN 672

Query: 130 HVPYRNSKLTQLLQDSLGGQAKTLMFVHISPEPDAVGETLSTLKFAERVATVELGAARVN 189
           H+PYRNSKLTQLLQD+LGGQAKTLMF+HISPE + +GETLSTLKFAERVATV+LGAARVN
Sbjct: 673 HIPYRNSKLTQLLQDALGGQAKTLMFIHISPELEDLGETLSTLKFAERVATVDLGAARVN 732

Query: 190 KDTSDVKELKEQIASLKAALARKEGAPQHVQHHASGNSEKFKSKATTELSPCQPKSQDVD 249
           KDTS+VKELKEQIASLK ALARKE      Q       +K   K +  +S    KS +  
Sbjct: 733 KDTSEVKELKEQIASLKLALARKESGADQTQLQRPLTPDKLLRKKSLGVSSSFSKSANST 792

Query: 250 VLVEHTIRRQPMGDVGNIELHNNSAIRQKRQSFDMDELLANSPPWPPVSSPCLNYGEDDK 309
             V+   +   + DV +IE  ++SA      S D+  L+  SP W    +P  +  E+D 
Sbjct: 793 RQVQTKHKPSQIDDVNSIEGQSDSA-----SSLDLQGLV-GSPSW---KTPPRDGKEEDM 852

Query: 310 DTV--SGEWVDKVMVNKQDVNQVENPLGCWEAENGHLNDVFYQKYLQ--DSSKLYTEQGY 369
           + +    EWVDK   ++ ++ +   P      +         ++  +  D +K  +    
Sbjct: 853 EFIIPGSEWVDK---HEDEITRSSKPENRAHTQLEKRTSSLKREATRGVDKNKCNSSVDK 912

Query: 370 GMLTGANRFNMAGIDDIDDLD-AGTSDSSEPDLLWQFNHSKLTSIGNGIGSKTK-KPNGG 429
           G+      +     ++ ++ D   TSD SE +L+WQ N           GS TK K N  
Sbjct: 913 GLEVRKIPYE----EEANESDETATSDCSETNLMWQLNVQVNMPRPASNGSSTKLKKNQS 972

Query: 430 KPVKSPELSKNFSSSMGPSPSQKIS 449
           K  +    +++   S+ P+P++ +S
Sbjct: 973 KISRVAAETRSMIPSLIPTPTRTLS 981

BLAST of Lag0031032 vs. TAIR 10
Match: AT3G44730.1 (kinesin-like protein 1 )

HSP 1 Score: 308.5 bits (789), Expect = 9.2e-84
Identity = 176/325 (54.15%), Postives = 231/325 (71.08%), Query Frame = 0

Query: 9   LEIRNSSQ-NGLSVPDANLVRVSSTSDVINLMNLGQRNRAVGATALNDRSSRSHSCLTVH 68
           L+IRN+SQ NGL+VPDANL+ VS+T DV++LM +GQ+NRAVGATALN+RSSRSHS LTVH
Sbjct: 532 LDIRNNSQLNGLNVPDANLIPVSNTRDVLDLMRIGQKNRAVGATALNERSSRSHSVLTVH 591

Query: 69  VQGRDLTSGAVLRGCMHLVDLAGSERVDKSEVTGDRLKEAQHINKSLSALGDVIASLAQK 128
           VQG++L SG++LRGC+HLVDLAGSERV+KSE  G+RLKEAQHINKSLSALGDVI +LAQK
Sbjct: 592 VQGKELASGSILRGCLHLVDLAGSERVEKSEAVGERLKEAQHINKSLSALGDVIYALAQK 651

Query: 129 NPHVPYRNSKLTQLLQDSLGGQAKTLMFVHISPEPDAVGETLSTLKFAERVATVELGAAR 188
           + HVPYRNSKLTQ+LQDSLGGQAKTLMFVHI+PE +AVGET+STLKFA+RVA++ELGAAR
Sbjct: 652 SSHVPYRNSKLTQVLQDSLGGQAKTLMFVHINPEVNAVGETISTLKFAQRVASIELGAAR 711

Query: 189 VNKDTSDVKELKEQIASLKAALARKEGAPQHVQHHASGNSEKFKSKATTELSPCQPKSQD 248
            NK+T ++++LK++I+SLK+A+ +KE   + ++  +  N+   + +    +SP       
Sbjct: 712 SNKETGEIRDLKDEISSLKSAMEKKEAELEQLRSGSIRNTT--ECQRARAVSPFHLPRTG 771

Query: 249 VDVLVEHTIRRQPMGDVGNIELHNNSAIRQKRQSFDMDELLAN---SPPWPPVSSPCLNY 308
                +     QP     + E  + S  +Q++  F     L N   SP  P ++   LN 
Sbjct: 772 NGAGTKAEASPQPNDGTRSYETRSCSTGKQRKSGF--PSALRNREASPRMPNLAEERLNP 831

Query: 309 GEDDKDTVSGEWVDKVMVNKQDVNQ 330
               +   +         NK DV Q
Sbjct: 832 SPSRRSLSTDRASTIKSRNKPDVTQ 852

BLAST of Lag0031032 vs. TAIR 10
Match: AT3G10310.1 (P-loop nucleoside triphosphate hydrolases superfamily protein with CH (Calponin Homology) domain )

HSP 1 Score: 276.9 bits (707), Expect = 3.0e-74
Identity = 153/242 (63.22%), Postives = 188/242 (77.69%), Query Frame = 0

Query: 6   IFTLEIR--NSSQNGLSVPDANLVRVSSTSDVINLMNLGQRNRAVGATALNDRSSRSHSC 65
           +F + IR  +S  +GLS+PDA +  V+ST DV+ LM  G+ NRAV +T++N+RSSRSHS 
Sbjct: 476 LFLIYIRTCSSDDDGLSLPDATMHSVNSTKDVLQLMEAGEVNRAVSSTSMNNRSSRSHSI 535

Query: 66  LTVHVQGRDLTSGAVLRGCMHLVDLAGSERVDKSEVTGDRLKEAQHINKSLSALGDVIAS 125
             VHV+G+D TSG  LR C+HLVDLAGSERVDKSEVTGDRLKEAQ+INKSLS LGDVI++
Sbjct: 536 FMVHVRGKD-TSGGTLRSCLHLVDLAGSERVDKSEVTGDRLKEAQYINKSLSCLGDVISA 595

Query: 126 LAQKNPHVPYRNSKLTQLLQDSLGGQAKTLMFVHISPEPDAVGETLSTLKFAERVATVEL 185
           LAQKN H+PYRNSKLT LLQDSLGGQAKTLMF H+SPE D+ GET+STLKFA+RV+TVEL
Sbjct: 596 LAQKNSHIPYRNSKLTLLLQDSLGGQAKTLMFAHLSPEEDSFGETISTLKFAQRVSTVEL 655

Query: 186 GAARVNKDTSDVKELKEQIASLKAALARKEGAPQHVQHHASGNSEKFKSK-----ATTEL 241
           GAAR +K+T +V  LKEQI +LK AL  +E       ++ S  S++ KS      ATTE 
Sbjct: 656 GAARAHKETREVMHLKEQIENLKRALGTEE------WNNVSNGSKEIKSPFSRPIATTER 710

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_008454311.27.0e-24392.75PREDICTED: kinesin-4 [Cucumis melo][more]
KAA0044397.12.0e-24292.54kinesin-4 [Cucumis melo var. makuwa][more]
TYK29525.13.5e-24292.32kinesin-4 [Cucumis melo var. makuwa][more]
QWT43339.11.1e-24093.30kinesin-like protein KIN14J [Citrullus lanatus subsp. vulgaris][more]
XP_022155292.14.2e-24092.36kinesin-like protein KIN-14I [Momordica charantia] >XP_022155293.1 kinesin-like ... [more]
Match NameE-valueIdentityDescription
F4IL572.2e-13560.63Kinesin-like protein KIN-14I OS=Arabidopsis thaliana OX=3702 GN=KIN14I PE=2 SV=1[more]
Q10MN52.9e-12756.63Kinesin-like protein KIN-14F OS=Oryza sativa subsp. japonica OX=39947 GN=KIN14F ... [more]
O816357.0e-10551.60Kinesin-like protein KIN-14G OS=Arabidopsis thaliana OX=3702 GN=KIN14G PE=1 SV=2[more]
Q0IMS91.7e-10350.32Kinesin-like protein KIN-14Q OS=Oryza sativa subsp. japonica OX=39947 GN=KIN14Q ... [more]
B9G8P16.2e-10149.68Kinesin-like protein KIN-14P OS=Oryza sativa subsp. japonica OX=39947 GN=KIN14P ... [more]
Match NameE-valueIdentityDescription
A0A1S3BZI83.4e-24392.75kinesin-4 OS=Cucumis melo OX=3656 GN=LOC103494750 PE=3 SV=1[more]
A0A5A7TRA69.8e-24392.54Kinesin-4 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold46G001260 PE=3... [more]
A0A5D3E2I51.7e-24292.32Kinesin-4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold655G001280 PE=... [more]
A0A6J1DMK62.0e-24092.36kinesin-like protein KIN-14I OS=Momordica charantia OX=3673 GN=LOC111022430 PE=3... [more]
A0A6J1F5B93.6e-23790.87kinesin-like protein KIN-14I isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC1114... [more]
Match NameE-valueIdentityDescription
AT2G47500.11.6e-13660.63P-loop nucleoside triphosphate hydrolases superfamily protein with CH (Calponin ... [more]
AT5G27000.15.0e-10651.60kinesin 4 [more]
AT1G09170.11.0e-9850.56P-loop nucleoside triphosphate hydrolases superfamily protein with CH (Calponin ... [more]
AT3G44730.19.2e-8454.15kinesin-like protein 1 [more]
AT3G10310.13.0e-7463.22P-loop nucleoside triphosphate hydrolases superfamily protein with CH (Calponin ... [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 188..215
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 212..242
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 409..477
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 430..452
NoneNo IPR availablePANTHERPTHR47972KINESIN-LIKE PROTEIN KLP-3coord: 9..473
NoneNo IPR availablePANTHERPTHR47972:SF24P-LOOP NUCLEOSIDE TRIPHOSPHATE HYDROLASE SUPERFAMILY PROTEINcoord: 9..473
IPR001752Kinesin motor domainPRINTSPR00380KINESINHEAVYcoord: 131..152
score: 67.12
coord: 82..100
score: 52.94
coord: 51..68
score: 49.54
IPR001752Kinesin motor domainSMARTSM00129kinesin_4coord: 1..189
e-value: 1.7E-37
score: 140.6
IPR001752Kinesin motor domainPFAMPF00225Kinesincoord: 8..180
e-value: 3.6E-61
score: 206.9
IPR001752Kinesin motor domainPROSITEPS50067KINESIN_MOTOR_2coord: 1..181
score: 73.916702
IPR036961Kinesin motor domain superfamilyGENE3D3.40.850.10Kinesin motor domaincoord: 6..222
e-value: 2.1E-77
score: 262.3
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILY52540P-loop containing nucleoside triphosphate hydrolasescoord: 8..214

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0031032.1Lag0031032.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0007018 microtubule-based movement
molecular_function GO:0005524 ATP binding
molecular_function GO:0008017 microtubule binding
molecular_function GO:0003777 microtubule motor activity