Lag0031011 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0031011
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionGamma-tubulin complex component
Locationchr11: 3820725 .. 3829046 (-)
RNA-Seq ExpressionLag0031011
SyntenyLag0031011
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTACACGAGCTTCTACTTGCTCTACTGGGTTACACCGGCGATCTGATAATCGACGAAAGAGAGCATTACAACTCTCTAGGGTTGAATCACTTAACTCCCGATGCCCCCATTTCCGGCGAACCCACATTCAAGCTCGCCCCCGATATCTCCTTTCTCGAACCGAGCGAAAGGTACGCCCACAACCCACTTCAAATGGATTCTTTTGTATAGATTGTCGATGATGGTTTTCTATTTTCGGATTCAGGGATCTAATTCAGAGGATCATTGTGTTAGGATTTTACTATAGAGAGCTCGACCGTTTTGCTACTAAGTCTAGGAATTTGAGTTGGATTCGTTCTGGCAATGGGTCTTTGTTAGCCAATTCTGCGGAGTCGTCTAAAGAAAAAACTGAAAACCCAAGTGTGTATAGGAGGGCCATAGCTAATGGCATTGTAGAGATACTGTCTGTTTATAGATCTGCTGTTCTTCATGTGGAGCAGAAACTGTTGTCTGAAACGGTGCCAATTTTGGCAGTAGTCACGCAGGGCCTCGATAAGGTTGGTTCTAAGATGATGCCCTTTGCTCTGTAGGTAATTTTGTTGTCCTAGCTTTTACTGTATTGGTCAATGGGTTTGATTGTAAGATTATGCCTTGAATTTGACTTGTAGATTGTATTTTCTAGTTCTTTGTTCTTTTTCCACCTCTGCATCAACTCATTTTAGAGATTGAGCGTGACGATATTCGAGGTGGCCAACTACTTAATCTTCTACACAAACGTTGTCACTGTGGTGTGCCTGAATTGCAGACTTGCATTCAAAGGTATTCAAGTCCTTTCCTGTTTCTTGCATGGAAAAAAAAACATCACATGAGAGTAATGTGATACCAGTGATCTCTCATTAGGGCTTAATCAGTGTTTTAAAAGGCTCAAGGCGCACTAAGGCGCAATGGCCTCCTGGGTCCTAGGCGCAAGGCGCACAAAAAGGCGCGGGCTTTATTATGTAAGGCGCACTATATAGAAAAAAATTTACAAAAATATATACATGTATATAAAACACTTTCATGGCAAGTAATGAAGTTTTTAACCAAGAAAAATAGATAAAAGATAAATAAGGTTCAAATATTAGTCTTCCAACATAGAGTCAAATGTCAATACTCCACAAAGTGAGTTTAGTATTGTTAATATGCTTTCCTTGTTGCTTGCTCATTTGTTTATTCACTCATGTATGAACAGCATAAGAGCTGGTATTCTTGGATTCTTTCTTCGCTTACAAGCTAAATTTAAAATTAGCTTCCTAAAAGTATTTATGTTTAAAATAAGTTAAAACATATATAAAATTGTGAAGCCCCAGAGGCGCCTAAGGCACGCGCCTTCCTCACTGCGCCTTGCGCCTAGGCGCGTCTTTTAAAACACTGGGCTTAATTAAGATGGTACTGGGTGTACACTCCTAGAAAGAATGAGCTTTCTATTAGCAACACATACTATGGTAGAATACATTTGAATCTAAGATTAACAGAGTACCTTACCTATTGAATGAGAATAGAAGTAAATGAGGAGACGAATAGGGAAGTTAAAAAGGAGAAGAAGAATAGGGAAGAAGGAATTTCAATATTCATTATTGTATTTAATAGTGGAGTTCTTAGATTTTGGTCCATGTTCTAATATTTCAATTCAATTGGAAGATGTACACTGGTTGGAAATTGCACTGTTCATTTCTGGTTCAAAAGAAACTGTATGTGCGAAATGTCCATCAATGCATATAGTTCCTCTCAAATGAATATTTAAATTGTTAATTCTTCATTGTAGATCATGCGGCAAGATTACTCTGCTGGTCTACTTGTTTTTGGAAGTTCATGCTTGATTCATTAAATTATTATTTTGTGTTTTTTTTCCTATCTGGCCCTCTGCGAAAAGAATAGGCTCCTTTGGCATGGTCATCAGGTATTGTACAATCAACTAGCGTCATGGATGGTTTATGGGATCCTTCAAGATAAGCATGGAGAATTTTTCATCAGAAGGTAACACTAAGAATTGTTGTATCTTATTATAGCAAACATGGGGTCGGATTGTGTATACATTGTTTCAGACTCTCAGTAGATAATAGTTCGACACGGTTGGTATTTGAGAGATCGCTTAAGGTCTCAATGATGCAGAAAGGGTCTTTGGTGGGGGTGCTGCCGTAATTGCTTAATAAATATGGTGAACGTTTGCTTAAGTCCATCTACACTATCTTATTTTGGCATCTGAGAACTGATGGCCTTATGGATGTATTTTTGAGTCAGTCCTTCAAAGGATTTTCCTTGATGAATATACTGTTTTATTCACAAGTAATAGAAATCTCATCCAGTGGGAATCATAGTTTCTCTTAAAATAAGAAAGAAGAAAGAAGAAATCTTGTCCTGTGAAAAAACTGTGAATTTGATATGGAGGAATTTTCATAATCGAGAAATACAATAAATACAATCTTCTTATCTATTATATCCCATGTGCTAAAGAAGAAGTTATCAAGAACCGAATGGGGTTACTGTTTGCAATTGTTCCCATAGCCCATCATACAAGATCTGGGATCTTGTCTTCTCTCATGAATGCCACTTAATGCAGAAATAGATACTTGGAATTTTATTATTAAAAAAACGAGTTAATTTTGAGGATCAGCTCACTTACAAAATTTTGGAATTTATTGTCATGGCAGTTGACCCGGTCTACTGCCAGATGAACGGACAACCTACTAAACACACAAACGAACTATTAACTAACTACTAGTCACTTTTACAACTCTCTCAGCTACGTGGTAATGGTATTTAGTCGTTGTACATGTGCTTTCTGATTTCTTATGTTGTACTATTTTTCACTTTATCATAGGAAAAATGTGTCTAGTTTCTGATCACATATGTCTTTTTCCTTGCTGGTGATAGCTGTTATATATATATATATATTATTTAGTCAAAGTAGAGTAAAAAATGTTTCACAAAGATAAAAGTAAAATATAACAAGCAAGTTTAATAGAGAGAGCTTACAAGGAAGAAATGAAACAAAGAAACAGTTCTGTAGAGAGGATAAATGGTCAATCTTGAAGTAACTCTTTAAGATTTAATTTTTCTTGTTCATATTCTTTTGTATGCATTTGATAATTCTTACATCCTAGAAATGCACGAGTCAATTTATGATCATCGAACTGCTCAGGCAGGATGACAGGGAGACTGAACAAGGCTCATCTGTACAAGACATGTCAGAGAAGTTAGGACGCTTATCAACTGATGAATCTTTGACTGATTGGCACTTGGGATTTCACATTTACCTGGTATATTCATTGCCACGTTCTACCAACATTACAGTTCCTTTTTTTTTTTTTTTTTTTTAATGATCACTGCTTTTAGTTCTTGTGTGGTAACTTAAATTTGACATGAATTTTGATGGCATTTTCAACGTCTGTGTGATTTGTTCTGAGTTCTTCTCGACTGGTTTTTTCACCTTCATTTTTCTTGTGATAGCTATTTGATTGTTACTTCTCTTTCTCCCCTTTTTGATAGATGACAATACTCCTCTTGAGGCAATCTTTTGGTTTATCTGAGGCTTTGGTCTCAACTCTCAAGAGATCTGTTCACTTGTTACCTGTCTTAACGAATTTCCTCCTCATTCTATACGATTGATAGTGGCACACATTGATTTCTCATGCATAAACTTTTCCATACAGCCCCACAATTTTCCCCATAAATTGGTTTCAGATTTTTAGACTGCAGAAATTGTTCAATAGCCTCATCCCTTTTACACATGAAAAGGAACTAACTATGCCATCCATCTCTTCCTTAATAATTACATTAGCTAGGAATCTAGGATCAACTTAGAGATATTGACTTTTAGACTCAATGAGAATTCAAAGATCTTAAGGAGTTTGAACCAATTTTGCATTTTTCATGTATTAGCTGAGTTGAAAATGATCTTGTCAACAATGAATTAAGAGAGAGATGGATGGATGGATGGATATCATCCCTCCCAATGGTGAGACCTTCAATGATGTTAGCCTCAACACTCTTTCCCCCAGGCTACTAAAATAATGAGTCACTAATGTTTATAAAAGAAAAGAACATGATAGGCAATAATCATATCCATTTCGAAGTAGAATGATGAGCAAATATGATAGCAAGGTCTTAAGTTGGCGGTAAAAGGAAGGGGCCAAGACTAAGAGGTTACCTAATTTAAGTATTGCTTTTACCTTCCTCTGTATGTTACCAACTTCCCTAGCAGTGTTGAATCTGGTGGGATATGTATTCGCTGTAATGTCAGAAAAGGCCTCTCTGGCTGTTTCTCTTCTCTCTGCCCTCCCCCCATAGTATGTACGAGGTTGATTGAATAATAATTTTCTCCATCGTCTTCTGAGCTGGAAGTGGGTTTGATACTGAATTTAATTGCCCACTAATATTTTCTTCTCTCACTCTGTTTTCAGGATATGCTTCCTGATTATATCCATATGCGGGTTGCAGAAGCAATTCTTTTTGCTGGTAAAGCCATTAGAGTTCTTCGGAACCCAAGCAATGCCTTTTGGTGTCAGGGTACTGGAAATCAGTCACATTCACAGATACCTAGACTACCTCTGAATATTAAAGGAAATACAAGGAACTTCCCCCTTCAAAAGGAACCTTTTGTTGCCACAAAGCTGACTGGAGAAGAATTACTTCTGCAATCTGAAGCAGATAAGATAGAAGCCATGCTTTTAGATCTCAAGGTTTATATAGCTTCGATTAGATTACCACTGGAACATAGCTTAACTTAAATTTGTTTGCTGTTTCTGCCTCACATATGTGCTTAAAAATGGTTCATTTATCTCTTCTCTAATACAACCCAATCACTTATTAGAGAAAATGGACTTTCATTATGTGCAGGAATCATCCGAGTTTCACAAGAGATCATTTGAGTCTGCTGTTGATTCAATTAGGGCTATTGCGGCTAGTCATCTTTGGCAGGTTGTGATTGTGGAACATTTAGTTCTACCTTTTATTTCATCATTTTATTATTGACTAATTCATATCTCTGCAGCTTGTAGTTGTACGTGCTGACCTCAATGGACACCTCAAGGCCCTAAAAGACTATTTCCTTTTAGCAAAAGGTGATTTCTTCCAGGTAAAAGATGGACTAATCTGAATTTAATTTCTTCTTTTGTTTATACTTCAACCAAGGATCTTTTATAGGCGATATGAAATTATAATTGTCCATTTGTATGTTGAGGAAGATTGGTTCCCAATTTCTGTGGTCCTTGAGAAGAAACTTTAATTAAGATGACGTGTGTTTATATTCAATATTCATCTTCAACTTGTAAAGCTGTTGGAATCTTTCGATGATACTCCATAGAAGAGGAATCTTAGTTGTCTTCAGAGGTAAATTAAAGTAACATTATTTAAGATATTAAGTTTATGCTTTCTAATTTAGAATTATTCATTTTCTGCTATCATTTCTATGGAATGTGATTAAGTTCACTTTGAGAATTTAAAGTTTTCATCACATGATATGCTAAATTTTCCACATCAACTTCTACAATGGGAATTGTGTTCCGAGTATTAGAATTTGATTACTTTTAAGCTTTCTTACATATGCATTTTCAAATTTTTGCTTTGAAATGGTGTCTGAGATGCTATTATCTCTACTTAATTTTAGCTTTGATCAAAATCTTTTGTTGCTATGAGCTAACTGGTGTTAGATCATTCCTTATTGTTTTTTCATATTATGCTAATACCATGTTTTGCTATGCTAGTGTTTTCTTGAGGAAAGTCGCCATTTGATGCGCCTACCCCCTCGCCAGTCGACTGCTGAAGCTGATCTTATGATCCCATTTCAGTTGGTAATTTTCCTTTTGGGCCAGACGTTCTCAACTATTCCAAATTCTTCTCTTACCAAAAGAAACTATTCAAATTCTGCATAATGTATTTGAAATGCAGATGTATCATTTAATTTGTGTTTGAATATTAAAAAAAAGTGTTTGAATAAACTTCAACATAATTTACATTCCATGTAGGCTGCGACAAAGACTACAAGTGAAGAAGACAAATACTTTTCAAGAGTATCATTGCGGTTAGTTGTGGTGTTTTTTTTTTAAATGGGGAACTAGCTTTCATCTAATATGATATTTGACTGGAAAATTGGTTTATATTTCTGATTACGTTATTTGAAGTTCTTAAACGTGTCTTTGGCAATCCTTTTCACTTCTACTAGGATGCCATCATTTGGAGTCAAAGTCAAGTCTGCACAAGGCGACTCGAAGGAAAAATCTTACACTGATGGAAACCTCGGTGGCGCTCTATCAAATTTGTCTTCAGATATGTCTCTTGATGGCTGGGATGGTGTTGCTCTTGAATACTACATTGATTGGCCCCTGCAGTTATTCTTTACTCAAGAAGTGCTCTCTAAGTAAGCATTGGCTGTTACTTTTCTTTTTTCTTTTTTCTTTTTGGATAATTGGAGTGTCGAGGAACCACTACTTGATGGCTGGGAGGACCTCGCTAATCTTCAAAGGCTTCGCCTACATCAAGGCCAGCTCCAAGAGACAAAAAGGCTTTAGTAAATTATGAATCTTTTGGTTGTCCCATTTGCTTTTAAGCAAAAGGATAGACTGAATACATTCTTATTAACAGAATTGCTTCACCTTTTGACCACTAACAAGATGAATGGTCCAGGTATTGCAGGGTCTTCCAGTATTTATTGCGGCTGAAACGAACACAGATGGAATTGGAGAAATCATGGGCGTCCCTAATGCACCAAGATCATGCTGATTTTGCCAAAAATCGCCGTGCTCGATTTGATGGTTCAATATCTTGGCAAAGGCAACGTTTTAGACGGATGTGGCGTGTAAGAGAACACATGGCATTCTTGATCAGAAATCTTCAGTTTTACATCCAGGTTAGAATCAATGCCTTCTTTTGACTTCAATATCATGTACTTTAAAGACCACTCATCATTTGTACCTAAATTAAATTTGTTATTCATCCCCTAGGTTGACGTGATTGAATCTCAATGGAATATTTTGCAAGACCATATCCAAGATTCTCACGACTTTACCGAGCTCGTGGGGTTTCATCAAGAGTACGTATATCAACTAATTTCATATGGTTTTTGCTTAAATCACATAGATTTTCTCAATAAGTCTTCTCTAAGAACTCATCTTTGCTATCCTTTCAAGCATGCACTATCAATGATTTGTCTCGCATGCATATTTCTTATTTTTTTTCCTTTTGATTAGTTGGGTCTGGATAAGCTGATCTTTGTAGGTTTCAGAGATAATCTATTGTAATAAATTGAAAACTTGTGCTAATGCATGTAAGAACCCGGTGCAGGTACTTATCCGCTCTAATTTCACAGTCATTCTTGGATATCGGTTCCGTTTCAAGGATACTGGATGGCATAATGAAGCTTTGCCTGCAGTTTTGCTGGAGTATAGAAAATCAAGACAGTAATTCAGACCCTCCTGAACTAGAGCACCTGACTGAGGTAACAATAACATATGGTTTACATATCTCTTGTAGATTTACAGAAAAAAAAAATTAGTTCATATCTCTTGATCAATTCCTACAATGGTATAGTGCCCTCATTATGTGACATGATTACACTTGATTTTAGGAATTCAACAAGAAATCCAACTCCCTATACACGATTTTGCGAAGTAGCAGGCTGGTTGGGAGTCAGCGGGCTCCATTCTTGAGACGTTTCTTGATGCGTTTGAATTTCAATTCCTTCTTCGAGGTAAAAATCTCGTCAATGTTTCTCTTCTCTTTTCTTGTAGCTAGGACTCCAAGATGTTTCGAAATACACTCAAGATGTTAGGTTAAAGTCTCTGGGTTGAAAACTTAGGCCCCGTTTGATAACCATTTGGTTTTTGAAAATTAAGCTTATAAACACATACTTCCACCTATAAGTTTCTTGGTTTTGGTATCAATCTTCTACCTTGTTTTCAAAAACCAAGTTCATAAAAAAAAAAAAAAGTAGTTTTCAAAAACTTGTTTTTGTTTTTAAAATTTGGCTAGGAATTCAAATGCAACATTAACTAGGATGAAAGCCATGGTAGGGAATTTAGGAGAAAACAAACATAATTTTCAAAAACCAAATAGTTATCAAACTGGGTCTTAATAACTTAAAACCTTTATCCCGCTATGAGCGATAATGCAAAGAGTGCAGCACCCGATACCTTGAATATTAAAAAAGTGTTTTCCTTATGGATTTTTAGTTTTGTAAAGTATGATGTTTGTTTTGTTTGTTGAATGTTTATTATGTTACTTGTACAACAGGCCACTGCAAGAGGGGTGCTAAACGTTGTTAGGCCACGTCCAGCAGCACTTCCAGTACTAAATCAACAATAG

mRNA sequence

ATGTTACACGAGCTTCTACTTGCTCTACTGGGTTACACCGGCGATCTGATAATCGACGAAAGAGAGCATTACAACTCTCTAGGGTTGAATCACTTAACTCCCGATGCCCCCATTTCCGGCGAACCCACATTCAAGCTCGCCCCCGATATCTCCTTTCTCGAACCGAGCGAAAGGGATCTAATTCAGAGGATCATTGTGTTAGGATTTTACTATAGAGAGCTCGACCGTTTTGCTACTAAGTCTAGGAATTTGAGTTGGATTCGTTCTGGCAATGGGTCTTTGTTAGCCAATTCTGCGGAGTCGTCTAAAGAAAAAACTGAAAACCCAAGTGTGTATAGGAGGGCCATAGCTAATGGCATTGTAGAGATACTGTCTGTTTATAGATCTGCTGTTCTTCATGTGGAGCAGAAACTGTTGTCTGAAACGGTGCCAATTTTGGCAGTAGTCACGCAGGGCCTCGATAAGTTCTTTGTTCTTTTTCCACCTCTGCATCAACTCATTTTAGAGATTGAGCGTGACGATATTCGAGGTGGCCAACTACTTAATCTTCTACACAAACGTTGTCACTGTGGTGTGCCTGAATTGCAGACTTGCATTCAAAGGCTCCTTTGGCATGGTCATCAGGTATTGTACAATCAACTAGCGTCATGGATGGTTTATGGGATCCTTCAAGATAAGCATGGAGAATTTTTCATCAGAAGGCAGGATGACAGGGAGACTGAACAAGGCTCATCTGTACAAGACATGTCAGAGAAGTTAGGACGCTTATCAACTGATGAATCTTTGACTGATTGGCACTTGGGATTTCACATTTACCTGGATATGCTTCCTGATTATATCCATATGCGGGTTGCAGAAGCAATTCTTTTTGCTGGTAAAGCCATTAGAGTTCTTCGGAACCCAAGCAATGCCTTTTGGTGTCAGGGTACTGGAAATCAGTCACATTCACAGATACCTAGACTACCTCTGAATATTAAAGGAAATACAAGGAACTTCCCCCTTCAAAAGGAACCTTTTGTTGCCACAAAGCTGACTGGAGAAGAATTACTTCTGCAATCTGAAGCAGATAAGATAGAAGCCATGCTTTTAGATCTCAAGGAATCATCCGAGTTTCACAAGAGATCATTTGAGTCTGCTGTTGATTCAATTAGGGCTATTGCGGCTAGTCATCTTTGGCAGCTTGTAGTTGTACGTGCTGACCTCAATGGACACCTCAAGGCCCTAAAAGACTATTTCCTTTTAGCAAAAGGTGATTTCTTCCAGTGTTTTCTTGAGGAAAGTCGCCATTTGATGCGCCTACCCCCTCGCCAGTCGACTGCTGAAGCTGATCTTATGATCCCATTTCAGTTGGCTGCGACAAAGACTACAAGTGAAGAAGACAAATACTTTTCAAGAGTATCATTGCGGATGCCATCATTTGGAGTCAAAGTCAAGTCTGCACAAGGCGACTCGAAGGAAAAATCTTACACTGATGGAAACCTCGGTGGCGCTCTATCAAATTTGTCTTCAGATATGTCTCTTGATGGCTGGGATGGTGTTGCTCTTGAATACTACATTGATTGGCCCCTGCAGTTATTCTTTACTCAAGAAGTGCTCTCTAAGTATTGCAGGGTCTTCCAGTATTTATTGCGGCTGAAACGAACACAGATGGAATTGGAGAAATCATGGGCGTCCCTAATGCACCAAGATCATGCTGATTTTGCCAAAAATCGCCGTGCTCGATTTGATGGTTCAATATCTTGGCAAAGGCAACGTTTTAGACGGATGTGGCGTGTAAGAGAACACATGGCATTCTTGATCAGAAATCTTCAGTTTTACATCCAGGTTGACGTGATTGAATCTCAATGGAATATTTTGCAAGACCATATCCAAGATTCTCACGACTTTACCGAGCTCGTGGGGTTTCATCAAGAGTACTTATCCGCTCTAATTTCACAGTCATTCTTGGATATCGGTTCCGTTTCAAGGATACTGGATGGCATAATGAAGCTTTGCCTGCAGTTTTGCTGGAGTATAGAAAATCAAGACAGTAATTCAGACCCTCCTGAACTAGAGCACCTGACTGAGGAATTCAACAAGAAATCCAACTCCCTATACACGATTTTGCGAAGTAGCAGGCTGGTTGGGAGTCAGCGGGCTCCATTCTTGAGACGTTTCTTGATGCGTTTGAATTTCAATTCCTTCTTCGAGGCCACTGCAAGAGGGGTGCTAAACGTTGTTAGGCCACGTCCAGCAGCACTTCCAGTACTAAATCAACAATAG

Coding sequence (CDS)

ATGTTACACGAGCTTCTACTTGCTCTACTGGGTTACACCGGCGATCTGATAATCGACGAAAGAGAGCATTACAACTCTCTAGGGTTGAATCACTTAACTCCCGATGCCCCCATTTCCGGCGAACCCACATTCAAGCTCGCCCCCGATATCTCCTTTCTCGAACCGAGCGAAAGGGATCTAATTCAGAGGATCATTGTGTTAGGATTTTACTATAGAGAGCTCGACCGTTTTGCTACTAAGTCTAGGAATTTGAGTTGGATTCGTTCTGGCAATGGGTCTTTGTTAGCCAATTCTGCGGAGTCGTCTAAAGAAAAAACTGAAAACCCAAGTGTGTATAGGAGGGCCATAGCTAATGGCATTGTAGAGATACTGTCTGTTTATAGATCTGCTGTTCTTCATGTGGAGCAGAAACTGTTGTCTGAAACGGTGCCAATTTTGGCAGTAGTCACGCAGGGCCTCGATAAGTTCTTTGTTCTTTTTCCACCTCTGCATCAACTCATTTTAGAGATTGAGCGTGACGATATTCGAGGTGGCCAACTACTTAATCTTCTACACAAACGTTGTCACTGTGGTGTGCCTGAATTGCAGACTTGCATTCAAAGGCTCCTTTGGCATGGTCATCAGGTATTGTACAATCAACTAGCGTCATGGATGGTTTATGGGATCCTTCAAGATAAGCATGGAGAATTTTTCATCAGAAGGCAGGATGACAGGGAGACTGAACAAGGCTCATCTGTACAAGACATGTCAGAGAAGTTAGGACGCTTATCAACTGATGAATCTTTGACTGATTGGCACTTGGGATTTCACATTTACCTGGATATGCTTCCTGATTATATCCATATGCGGGTTGCAGAAGCAATTCTTTTTGCTGGTAAAGCCATTAGAGTTCTTCGGAACCCAAGCAATGCCTTTTGGTGTCAGGGTACTGGAAATCAGTCACATTCACAGATACCTAGACTACCTCTGAATATTAAAGGAAATACAAGGAACTTCCCCCTTCAAAAGGAACCTTTTGTTGCCACAAAGCTGACTGGAGAAGAATTACTTCTGCAATCTGAAGCAGATAAGATAGAAGCCATGCTTTTAGATCTCAAGGAATCATCCGAGTTTCACAAGAGATCATTTGAGTCTGCTGTTGATTCAATTAGGGCTATTGCGGCTAGTCATCTTTGGCAGCTTGTAGTTGTACGTGCTGACCTCAATGGACACCTCAAGGCCCTAAAAGACTATTTCCTTTTAGCAAAAGGTGATTTCTTCCAGTGTTTTCTTGAGGAAAGTCGCCATTTGATGCGCCTACCCCCTCGCCAGTCGACTGCTGAAGCTGATCTTATGATCCCATTTCAGTTGGCTGCGACAAAGACTACAAGTGAAGAAGACAAATACTTTTCAAGAGTATCATTGCGGATGCCATCATTTGGAGTCAAAGTCAAGTCTGCACAAGGCGACTCGAAGGAAAAATCTTACACTGATGGAAACCTCGGTGGCGCTCTATCAAATTTGTCTTCAGATATGTCTCTTGATGGCTGGGATGGTGTTGCTCTTGAATACTACATTGATTGGCCCCTGCAGTTATTCTTTACTCAAGAAGTGCTCTCTAAGTATTGCAGGGTCTTCCAGTATTTATTGCGGCTGAAACGAACACAGATGGAATTGGAGAAATCATGGGCGTCCCTAATGCACCAAGATCATGCTGATTTTGCCAAAAATCGCCGTGCTCGATTTGATGGTTCAATATCTTGGCAAAGGCAACGTTTTAGACGGATGTGGCGTGTAAGAGAACACATGGCATTCTTGATCAGAAATCTTCAGTTTTACATCCAGGTTGACGTGATTGAATCTCAATGGAATATTTTGCAAGACCATATCCAAGATTCTCACGACTTTACCGAGCTCGTGGGGTTTCATCAAGAGTACTTATCCGCTCTAATTTCACAGTCATTCTTGGATATCGGTTCCGTTTCAAGGATACTGGATGGCATAATGAAGCTTTGCCTGCAGTTTTGCTGGAGTATAGAAAATCAAGACAGTAATTCAGACCCTCCTGAACTAGAGCACCTGACTGAGGAATTCAACAAGAAATCCAACTCCCTATACACGATTTTGCGAAGTAGCAGGCTGGTTGGGAGTCAGCGGGCTCCATTCTTGAGACGTTTCTTGATGCGTTTGAATTTCAATTCCTTCTTCGAGGCCACTGCAAGAGGGGTGCTAAACGTTGTTAGGCCACGTCCAGCAGCACTTCCAGTACTAAATCAACAATAG

Protein sequence

MLHELLLALLGYTGDLIIDEREHYNSLGLNHLTPDAPISGEPTFKLAPDISFLEPSERDLIQRIIVLGFYYRELDRFATKSRNLSWIRSGNGSLLANSAESSKEKTENPSVYRRAIANGIVEILSVYRSAVLHVEQKLLSETVPILAVVTQGLDKFFVLFPPLHQLILEIERDDIRGGQLLNLLHKRCHCGVPELQTCIQRLLWHGHQVLYNQLASWMVYGILQDKHGEFFIRRQDDRETEQGSSVQDMSEKLGRLSTDESLTDWHLGFHIYLDMLPDYIHMRVAEAILFAGKAIRVLRNPSNAFWCQGTGNQSHSQIPRLPLNIKGNTRNFPLQKEPFVATKLTGEELLLQSEADKIEAMLLDLKESSEFHKRSFESAVDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDFFQCFLEESRHLMRLPPRQSTAEADLMIPFQLAATKTTSEEDKYFSRVSLRMPSFGVKVKSAQGDSKEKSYTDGNLGGALSNLSSDMSLDGWDGVALEYYIDWPLQLFFTQEVLSKYCRVFQYLLRLKRTQMELEKSWASLMHQDHADFAKNRRARFDGSISWQRQRFRRMWRVREHMAFLIRNLQFYIQVDVIESQWNILQDHIQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRILDGIMKLCLQFCWSIENQDSNSDPPELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFLRRFLMRLNFNSFFEATARGVLNVVRPRPAALPVLNQQ
Homology
BLAST of Lag0031011 vs. NCBI nr
Match: XP_038879485.1 (gamma-tubulin complex component 4 homolog [Benincasa hispida])

HSP 1 Score: 1419.1 bits (3672), Expect = 0.0e+00
Identity = 716/754 (94.96%), Postives = 730/754 (96.82%), Query Frame = 0

Query: 1   MLHELLLALLGYTGDLIIDEREHYNSLGLNHLTPDAPISGEPTFKLAPDISFLEPSERDL 60
           MLHELLL+LLGYTGDLIIDEREHYNSLGLNHL  DAPISGEPTF LAPDISFLEPSERDL
Sbjct: 1   MLHELLLSLLGYTGDLIIDEREHYNSLGLNHLPLDAPISGEPTFTLAPDISFLEPSERDL 60

Query: 61  IQRIIVLGFYYRELDRFATKSRNLSWIRSGNGSLLANSAESSKEKTENPSVYRRAIANGI 120
           IQRIIVLGFYYRELDRFATKSRNLSWIRSGNGS LANS ESSK+K ENPSVYRRAIANGI
Sbjct: 61  IQRIIVLGFYYRELDRFATKSRNLSWIRSGNGSSLANSTESSKDKIENPSVYRRAIANGI 120

Query: 121 VEILSVYRSAVLHVEQKLLSETVPILAVVTQGLDKFFVLFPPLHQLILEIERDDIRGGQL 180
           VEILS+YRSAVLHVEQKLLSETVPILA+VTQGLDKF+VLFPPLHQLILEIE DDIRGGQL
Sbjct: 121 VEILSIYRSAVLHVEQKLLSETVPILAIVTQGLDKFYVLFPPLHQLILEIEHDDIRGGQL 180

Query: 181 LNLLHKRCHCGVPELQTCIQRLLWHGHQVLYNQLASWMVYGILQDKHGEFFIRRQDDRET 240
           LNLLHKRCHCGVPELQTCIQRLLWHGHQV+YNQLASWMVYGILQDKHGEFFIRRQDDRET
Sbjct: 181 LNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYGILQDKHGEFFIRRQDDRET 240

Query: 241 EQGSSVQDMSEKLGRLSTDESLTDWHLGFHIYLDMLPDYIHMRVAEAILFAGKAIRVLRN 300
            QGSSVQD+SEKLGRLSTDESLTDWHLGFHI LDMLPDYIHMRVAE+ILFAGKAIRVLRN
Sbjct: 241 NQGSSVQDVSEKLGRLSTDESLTDWHLGFHICLDMLPDYIHMRVAESILFAGKAIRVLRN 300

Query: 301 PSNAFWCQGTGNQSHSQIPRLPLNIKGNTRNFPLQKEPFVATKLTGEELLLQSEADKIEA 360
           PSNAFWCQG GNQSHS +PRLPLNIKGNTRNFPLQKEPFVATKLTGEELLLQSEADKIEA
Sbjct: 301 PSNAFWCQGAGNQSHSHMPRLPLNIKGNTRNFPLQKEPFVATKLTGEELLLQSEADKIEA 360

Query: 361 MLLDLKESSEFHKRSFESAVDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDFF 420
           MLLDLKESSEF KRSFESAVDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDFF
Sbjct: 361 MLLDLKESSEFRKRSFESAVDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDFF 420

Query: 421 QCFLEESRHLMRLPPRQSTAEADLMIPFQLAATKTTSEEDKYFSRVSLRMPSFGVKVKSA 480
           QCFLEESRHLMRLPPRQSTAEADLMIPFQLAATKTTSEED+YFSRVSLRMPSFGVKVKSA
Sbjct: 421 QCFLEESRHLMRLPPRQSTAEADLMIPFQLAATKTTSEEDRYFSRVSLRMPSFGVKVKSA 480

Query: 481 QGDSKEKSYTDGNLGGALSNLSSDMSLDGWDGVALEYYIDWPLQLFFTQEVLSKYCRVFQ 540
           QGD KEK + DGN GGALSNL  DMSLDGWDGVALEYYIDWPLQLFFTQEVLSKYCRVFQ
Sbjct: 481 QGDPKEKPFIDGNPGGALSNLPLDMSLDGWDGVALEYYIDWPLQLFFTQEVLSKYCRVFQ 540

Query: 541 YLLRLKRTQMELEKSWASLMHQDHADFAKNRRARFDGSIS-WQRQRFRRMWRVREHMAFL 600
           YLLRLKRTQMELEKSWASLMHQDHADFA NR A+FDGSIS  QRQRFRRMWRVREHMAFL
Sbjct: 541 YLLRLKRTQMELEKSWASLMHQDHADFANNRNAQFDGSISPQQRQRFRRMWRVREHMAFL 600

Query: 601 IRNLQFYIQVDVIESQWNILQDHIQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRILD 660
           IRNLQFYIQVDVIESQWNILQDHIQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRILD
Sbjct: 601 IRNLQFYIQVDVIESQWNILQDHIQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRILD 660

Query: 661 GIMKLCLQFCWSIENQDSNSDPPELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFLRRF 720
           GIMKLCLQFCWS+ENQD +SDP ELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFLRRF
Sbjct: 661 GIMKLCLQFCWSLENQDGSSDPSELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFLRRF 720

Query: 721 LMRLNFNSFFEATARGVLNVVRPRPAALPVLNQQ 754
           LMRLNFNSFFEATARGVLNVVRPRPAALPVLNQQ
Sbjct: 721 LMRLNFNSFFEATARGVLNVVRPRPAALPVLNQQ 754

BLAST of Lag0031011 vs. NCBI nr
Match: XP_011660150.1 (gamma-tubulin complex component 4 homolog isoform X1 [Cucumis sativus] >XP_011660151.1 gamma-tubulin complex component 4 homolog isoform X1 [Cucumis sativus] >XP_031744625.1 gamma-tubulin complex component 4 homolog isoform X1 [Cucumis sativus] >KGN66813.2 hypothetical protein Csa_007116 [Cucumis sativus])

HSP 1 Score: 1417.5 bits (3668), Expect = 0.0e+00
Identity = 716/754 (94.96%), Postives = 731/754 (96.95%), Query Frame = 0

Query: 1   MLHELLLALLGYTGDLIIDEREHYNSLGLNHLTPDAPISGEPTFKLAPDISFLEPSERDL 60
           MLHELLLALLGYTGDLIIDEREHYNSLGL++L  DAPISGEPTFKLA DISFLEPSERDL
Sbjct: 1   MLHELLLALLGYTGDLIIDEREHYNSLGLDNLLVDAPISGEPTFKLASDISFLEPSERDL 60

Query: 61  IQRIIVLGFYYRELDRFATKSRNLSWIRSGNGSLLANSAESSKEKTENPSVYRRAIANGI 120
           IQRIIVLGFYYRELDRFATKSRNLSWIRSGN S LANS ESSK+K ENPSVYRRAIANGI
Sbjct: 61  IQRIIVLGFYYRELDRFATKSRNLSWIRSGNESSLANSTESSKDKIENPSVYRRAIANGI 120

Query: 121 VEILSVYRSAVLHVEQKLLSETVPILAVVTQGLDKFFVLFPPLHQLILEIERDDIRGGQL 180
           VEILS+YRSAVLHVEQKLLSE VPILA+VTQGLDKFFVLFPPLHQLI EIERDDIRGGQL
Sbjct: 121 VEILSIYRSAVLHVEQKLLSEMVPILAIVTQGLDKFFVLFPPLHQLIREIERDDIRGGQL 180

Query: 181 LNLLHKRCHCGVPELQTCIQRLLWHGHQVLYNQLASWMVYGILQDKHGEFFIRRQDDRET 240
           LNLLHKRCHCGVPELQTCIQRLLWHGHQV+YNQLASWMVYG+LQDKHGEFFIRRQDDRET
Sbjct: 181 LNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYGLLQDKHGEFFIRRQDDRET 240

Query: 241 EQGSSVQDMSEKLGRLSTDESLTDWHLGFHIYLDMLPDYIHMRVAEAILFAGKAIRVLRN 300
            QGSSVQDMSEKLGRLSTDESLTDWHLGFHIYLDMLPDYIHMRVAE+ILFAGKAIRVLRN
Sbjct: 241 NQGSSVQDMSEKLGRLSTDESLTDWHLGFHIYLDMLPDYIHMRVAESILFAGKAIRVLRN 300

Query: 301 PSNAFWCQGTGNQSHSQIPRLPLNIKGNTRNFPLQKEPFVATKLTGEELLLQSEADKIEA 360
           PSNAFWCQG GNQSHS +PRLPLN+KGNTRNFPLQKEPFVATKLTGEELLLQSEADKIEA
Sbjct: 301 PSNAFWCQGAGNQSHSNMPRLPLNVKGNTRNFPLQKEPFVATKLTGEELLLQSEADKIEA 360

Query: 361 MLLDLKESSEFHKRSFESAVDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDFF 420
           MLLDLKESSEFHKRSFESAVDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDFF
Sbjct: 361 MLLDLKESSEFHKRSFESAVDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDFF 420

Query: 421 QCFLEESRHLMRLPPRQSTAEADLMIPFQLAATKTTSEEDKYFSRVSLRMPSFGVKVKSA 480
           QCFLEESRHLMRLPPRQSTAEADLMIPFQLAATKTTSEED+YF RVSLRMPSFGVKVKSA
Sbjct: 421 QCFLEESRHLMRLPPRQSTAEADLMIPFQLAATKTTSEEDRYFCRVSLRMPSFGVKVKSA 480

Query: 481 QGDSKEKSYTDGNLGGALSNLSSDMSLDGWDGVALEYYIDWPLQLFFTQEVLSKYCRVFQ 540
           QGD KEK   DGN GGALSNLS DMSLDGWDGV+LEYYIDWPLQLFFTQEVLSKYCRVFQ
Sbjct: 481 QGDLKEKPIIDGNPGGALSNLSLDMSLDGWDGVSLEYYIDWPLQLFFTQEVLSKYCRVFQ 540

Query: 541 YLLRLKRTQMELEKSWASLMHQDHADFAKNRRARFDGSISWQ-RQRFRRMWRVREHMAFL 600
           YLLRLKRTQMELEKSWASLMHQDHADFA NR+A+FDGSIS Q RQRFRRMWRVREHMAFL
Sbjct: 541 YLLRLKRTQMELEKSWASLMHQDHADFANNRKAQFDGSISLQRRQRFRRMWRVREHMAFL 600

Query: 601 IRNLQFYIQVDVIESQWNILQDHIQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRILD 660
           IRNLQFYIQVDVIESQWNILQDHIQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRILD
Sbjct: 601 IRNLQFYIQVDVIESQWNILQDHIQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRILD 660

Query: 661 GIMKLCLQFCWSIENQDSNSDPPELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFLRRF 720
           GIMKLCLQFCWSIENQDS+SDP ELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFLRRF
Sbjct: 661 GIMKLCLQFCWSIENQDSSSDPSELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFLRRF 720

Query: 721 LMRLNFNSFFEATARGVLNVVRPRPAALPVLNQQ 754
           LMRLNFNSFFEATARGVLNVVRPRPAALPVLNQQ
Sbjct: 721 LMRLNFNSFFEATARGVLNVVRPRPAALPVLNQQ 754

BLAST of Lag0031011 vs. NCBI nr
Match: XP_022934806.1 (gamma-tubulin complex component 4 homolog [Cucurbita moschata] >XP_022934807.1 gamma-tubulin complex component 4 homolog [Cucurbita moschata])

HSP 1 Score: 1416.7 bits (3666), Expect = 0.0e+00
Identity = 714/754 (94.69%), Postives = 731/754 (96.95%), Query Frame = 0

Query: 1   MLHELLLALLGYTGDLIIDEREHYNSLGLNHLTPDAPISGEPTFKLAPDISFLEPSERDL 60
           MLHELLLALLGYTGDLIIDEREHYN LGLNHL PDAPISGEPTFKLAPDISFLEPSERDL
Sbjct: 1   MLHELLLALLGYTGDLIIDEREHYNLLGLNHLPPDAPISGEPTFKLAPDISFLEPSERDL 60

Query: 61  IQRIIVLGFYYRELDRFATKSRNLSWIRSGNGSLLANSAESSKEKTENPSVYRRAIANGI 120
           IQRIIVLGFYYRELDRFATKSRNLSWIRS NGS L NS ESSK+KTE PSVYRRAIANGI
Sbjct: 61  IQRIIVLGFYYRELDRFATKSRNLSWIRSSNGSSLPNSVESSKDKTEIPSVYRRAIANGI 120

Query: 121 VEILSVYRSAVLHVEQKLLSETVPILAVVTQGLDKFFVLFPPLHQLILEIERDDIRGGQL 180
           VEILSVYRSAVLHVEQKLLSETVPILA+VTQGLDKFFVLFPPLHQL+LEIERDDIRGGQL
Sbjct: 121 VEILSVYRSAVLHVEQKLLSETVPILAIVTQGLDKFFVLFPPLHQLVLEIERDDIRGGQL 180

Query: 181 LNLLHKRCHCGVPELQTCIQRLLWHGHQVLYNQLASWMVYGILQDKHGEFFIRRQDDRET 240
           LNLLHKRCHCGVPELQTCIQRLLWHGHQV+YNQLASWMVYGILQDKHGEFFIRRQD RET
Sbjct: 181 LNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYGILQDKHGEFFIRRQDGRET 240

Query: 241 EQGSSVQDMSEKLGRLSTDESLTDWHLGFHIYLDMLPDYIHMRVAEAILFAGKAIRVLRN 300
           +QGS+VQ +SEKLGRLSTDESLTDWHLGFHIYLDMLPDYIHM+VAE+ILFAGKAIRVLRN
Sbjct: 241 DQGSAVQVLSEKLGRLSTDESLTDWHLGFHIYLDMLPDYIHMKVAESILFAGKAIRVLRN 300

Query: 301 PSNAFWCQGTGNQSHSQIPRLPLNIKGNTRNFPLQKEPFVATKLTGEELLLQSEADKIEA 360
           PSNAFWCQG GNQSHS +PRLPL IKGNTRNFPLQKEPF ATKLTGEELLLQSEADKIEA
Sbjct: 301 PSNAFWCQGAGNQSHSHMPRLPLKIKGNTRNFPLQKEPFFATKLTGEELLLQSEADKIEA 360

Query: 361 MLLDLKESSEFHKRSFESAVDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDFF 420
           MLLDLKESSEFHKRSFESA+DSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDFF
Sbjct: 361 MLLDLKESSEFHKRSFESAIDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDFF 420

Query: 421 QCFLEESRHLMRLPPRQSTAEADLMIPFQLAATKTTSEEDKYFSRVSLRMPSFGVKVKSA 480
           QCFLEESRHLMRLPPRQSTAEADLMIPFQLAATKTTS+ED+YFSRVSLRMPSFGVKVKSA
Sbjct: 421 QCFLEESRHLMRLPPRQSTAEADLMIPFQLAATKTTSDEDRYFSRVSLRMPSFGVKVKSA 480

Query: 481 QGDSKEKSYTDGNLGGALSNLSSDMSLDGWDGVALEYYIDWPLQLFFTQEVLSKYCRVFQ 540
           QGD KEK+YTDGN GGALSNLSSDMSLDGWD VALEYYIDWPLQLFFTQEVLSKYCRVFQ
Sbjct: 481 QGDPKEKTYTDGNPGGALSNLSSDMSLDGWDSVALEYYIDWPLQLFFTQEVLSKYCRVFQ 540

Query: 541 YLLRLKRTQMELEKSWASLMHQDHADFAKNRRARFDGSISWQ-RQRFRRMWRVREHMAFL 600
           YLLRLKRTQMELEKSWASLMHQDHADF+KNR ARFDGSIS Q RQRFR+MWRVREHMAFL
Sbjct: 541 YLLRLKRTQMELEKSWASLMHQDHADFSKNRNARFDGSISPQKRQRFRQMWRVREHMAFL 600

Query: 601 IRNLQFYIQVDVIESQWNILQDHIQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRILD 660
           IRNLQFYIQVDVIESQWNILQD IQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRILD
Sbjct: 601 IRNLQFYIQVDVIESQWNILQDRIQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRILD 660

Query: 661 GIMKLCLQFCWSIENQDSNSDPPELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFLRRF 720
           GIMKLCLQFCWSIENQD +SDP ELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFLRRF
Sbjct: 661 GIMKLCLQFCWSIENQDKSSDPSELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFLRRF 720

Query: 721 LMRLNFNSFFEATARGVLNVVRPRPAALPVLNQQ 754
           LMRLNFNSFFEATARGVLNVV PRPAALPVLNQQ
Sbjct: 721 LMRLNFNSFFEATARGVLNVVGPRPAALPVLNQQ 754

BLAST of Lag0031011 vs. NCBI nr
Match: XP_023528229.1 (gamma-tubulin complex component 4 homolog [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1414.4 bits (3660), Expect = 0.0e+00
Identity = 712/754 (94.43%), Postives = 730/754 (96.82%), Query Frame = 0

Query: 1   MLHELLLALLGYTGDLIIDEREHYNSLGLNHLTPDAPISGEPTFKLAPDISFLEPSERDL 60
           MLHELLLALLGYTGDLIIDEREHYN LGLNHL PDAPISGEPTFKLAPDISFLEPSERDL
Sbjct: 1   MLHELLLALLGYTGDLIIDEREHYNLLGLNHLPPDAPISGEPTFKLAPDISFLEPSERDL 60

Query: 61  IQRIIVLGFYYRELDRFATKSRNLSWIRSGNGSLLANSAESSKEKTENPSVYRRAIANGI 120
           IQRIIV GFYYRELDRFATKSRNLSWIRS NGS L NS ESSK+KTE PSVYRRAIANGI
Sbjct: 61  IQRIIVFGFYYRELDRFATKSRNLSWIRSSNGSSLPNSVESSKDKTEIPSVYRRAIANGI 120

Query: 121 VEILSVYRSAVLHVEQKLLSETVPILAVVTQGLDKFFVLFPPLHQLILEIERDDIRGGQL 180
           VEILSVYRSAVLHVEQKLLSETVPILA+VTQGLDKFFVLFPPLHQL+LEIERDDIRGGQL
Sbjct: 121 VEILSVYRSAVLHVEQKLLSETVPILAIVTQGLDKFFVLFPPLHQLVLEIERDDIRGGQL 180

Query: 181 LNLLHKRCHCGVPELQTCIQRLLWHGHQVLYNQLASWMVYGILQDKHGEFFIRRQDDRET 240
           LNLLHKRCHCGVPELQTCIQRLLWHGHQV+YNQLASWMVYGILQDKHGEFFIRRQD RET
Sbjct: 181 LNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYGILQDKHGEFFIRRQDGRET 240

Query: 241 EQGSSVQDMSEKLGRLSTDESLTDWHLGFHIYLDMLPDYIHMRVAEAILFAGKAIRVLRN 300
           +QGS+VQ +SEKLGRLSTDESLTDWHLGFHIYLDMLPDYIHM+VAE+ILFAGKAIRVLRN
Sbjct: 241 DQGSAVQVLSEKLGRLSTDESLTDWHLGFHIYLDMLPDYIHMKVAESILFAGKAIRVLRN 300

Query: 301 PSNAFWCQGTGNQSHSQIPRLPLNIKGNTRNFPLQKEPFVATKLTGEELLLQSEADKIEA 360
           PSNAFWCQG GNQSHS +PRLPL IKGNTRNFPLQKEPF ATKLTGEELLLQSEADKIEA
Sbjct: 301 PSNAFWCQGAGNQSHSHMPRLPLKIKGNTRNFPLQKEPFFATKLTGEELLLQSEADKIEA 360

Query: 361 MLLDLKESSEFHKRSFESAVDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDFF 420
           MLLDLKESSEFHKRSFESA+DSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDFF
Sbjct: 361 MLLDLKESSEFHKRSFESAIDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDFF 420

Query: 421 QCFLEESRHLMRLPPRQSTAEADLMIPFQLAATKTTSEEDKYFSRVSLRMPSFGVKVKSA 480
           QCFLEESRHLMRLPPRQSTAEADLMIPFQLAATKTTS+ED+YFSRVSLRMPSFGVKVKSA
Sbjct: 421 QCFLEESRHLMRLPPRQSTAEADLMIPFQLAATKTTSDEDRYFSRVSLRMPSFGVKVKSA 480

Query: 481 QGDSKEKSYTDGNLGGALSNLSSDMSLDGWDGVALEYYIDWPLQLFFTQEVLSKYCRVFQ 540
           QGD KEK+YTDGN GGALSN+SSDMSLDGWDGVALEYYIDWPLQLFFTQEVLSKYCRVFQ
Sbjct: 481 QGDPKEKTYTDGNPGGALSNISSDMSLDGWDGVALEYYIDWPLQLFFTQEVLSKYCRVFQ 540

Query: 541 YLLRLKRTQMELEKSWASLMHQDHADFAKNRRARFDGSISWQ-RQRFRRMWRVREHMAFL 600
           YLLRLKRTQMELEKSWASLMHQDHADF+KNR  RFDGSIS Q RQRFR+MWRVREHMAFL
Sbjct: 541 YLLRLKRTQMELEKSWASLMHQDHADFSKNRNDRFDGSISPQKRQRFRQMWRVREHMAFL 600

Query: 601 IRNLQFYIQVDVIESQWNILQDHIQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRILD 660
           IRNLQFYIQVDVIESQWNILQD IQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRILD
Sbjct: 601 IRNLQFYIQVDVIESQWNILQDRIQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRILD 660

Query: 661 GIMKLCLQFCWSIENQDSNSDPPELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFLRRF 720
           GIMKLCLQFCWSIENQD +SDP ELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFLRRF
Sbjct: 661 GIMKLCLQFCWSIENQDKSSDPSELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFLRRF 720

Query: 721 LMRLNFNSFFEATARGVLNVVRPRPAALPVLNQQ 754
           LMRLNFNSFFEATARGVLNVV PRPAALPVLNQQ
Sbjct: 721 LMRLNFNSFFEATARGVLNVVGPRPAALPVLNQQ 754

BLAST of Lag0031011 vs. NCBI nr
Match: KAA0035230.1 (gamma-tubulin complex component 4-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 1414.1 bits (3659), Expect = 0.0e+00
Identity = 716/755 (94.83%), Postives = 731/755 (96.82%), Query Frame = 0

Query: 1   MLHELLLALLGYTGDLIIDEREHYNSLGLNHLTPDAPISGEPTFKLAPDISFLEPSERDL 60
           MLHELLLALLGYTGDLIIDEREHYNSLGLN+L  DAPISGEPTFKLA DISFLEPSERDL
Sbjct: 1   MLHELLLALLGYTGDLIIDEREHYNSLGLNNLLVDAPISGEPTFKLASDISFLEPSERDL 60

Query: 61  IQRIIVLGFYYRELDRFATKSRNLSWIRSGNGSLLANSAESSKEKTENPSVYRRAIANGI 120
           IQRIIVLGFYYRELDRFATKSRNLSWIRSGN S LANS ESSK+K ENPSVYRRAIANGI
Sbjct: 61  IQRIIVLGFYYRELDRFATKSRNLSWIRSGNESSLANSTESSKDKIENPSVYRRAIANGI 120

Query: 121 VEILSVYRSAVLHVEQKLLSETVPILAVVTQGLDKFFVLFPPLHQLILEIERDDIRGGQL 180
           VEILS+YRSAVLHVEQKLLSETVPILA+VTQGLDKFFVLFPPLHQLI EIERDDI+GGQL
Sbjct: 121 VEILSIYRSAVLHVEQKLLSETVPILAIVTQGLDKFFVLFPPLHQLIREIERDDIQGGQL 180

Query: 181 LNLLHKRCHCGVPELQTCIQRLLWHGHQVLYNQLASWMVYGILQDKHGEFFIRRQDDRET 240
           LNLLHKRCHCGVPELQTCIQRLLWHGHQV+YNQLASWMVYG+LQDKHGEFFIRRQDDRET
Sbjct: 181 LNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYGLLQDKHGEFFIRRQDDRET 240

Query: 241 EQGSSVQDMSEKLGRLSTDESLTDWHLGFHIYLDMLPDYIHMRVAEAILFAGKAIRVLRN 300
            QGSSVQDMSEKLGRLSTDESLTDWHLGFHIYLDMLPDYIHMRVAE+ILFAGKAIRVLRN
Sbjct: 241 NQGSSVQDMSEKLGRLSTDESLTDWHLGFHIYLDMLPDYIHMRVAESILFAGKAIRVLRN 300

Query: 301 PSNAFWCQGTGNQSHSQIPRLPLNIKGNTRNFPLQKEPFVATKLTGEELLLQSEADKIEA 360
           PSNAFWCQ  GNQSHS +PRLPLN+KGNTRNFPLQKEPFVATKLTGEELLLQSEADKIEA
Sbjct: 301 PSNAFWCQSAGNQSHSNMPRLPLNVKGNTRNFPLQKEPFVATKLTGEELLLQSEADKIEA 360

Query: 361 MLLDLKESSEFHKRSFESAVDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDFF 420
           MLLDLKESSEFHKRSFESAVDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDFF
Sbjct: 361 MLLDLKESSEFHKRSFESAVDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDFF 420

Query: 421 QCFLEESRHLMRLPPRQSTAEADLMIPFQLAATKTTSEEDKYFSRVSLRMPSFGVKVKSA 480
           QCFLEESRHLMRLPPRQSTAEADLMIPFQLAATKTTSEED+YFSRVSLRMPSFGVKVKSA
Sbjct: 421 QCFLEESRHLMRLPPRQSTAEADLMIPFQLAATKTTSEEDRYFSRVSLRMPSFGVKVKSA 480

Query: 481 QGDSKEKSYTDGNLGGAL-SNLSSDMSLDGWDGVALEYYIDWPLQLFFTQEVLSKYCRVF 540
           QGD KEK   DGN GGAL SNLS DMSLDGWDGV+LEYYIDWPLQLFFTQEVLSKYCRVF
Sbjct: 481 QGDLKEKPIIDGNPGGALSSNLSLDMSLDGWDGVSLEYYIDWPLQLFFTQEVLSKYCRVF 540

Query: 541 QYLLRLKRTQMELEKSWASLMHQDHADFAKNRRARFDGSISWQ-RQRFRRMWRVREHMAF 600
           QYLLRLKRTQMELEKSWASLMHQDHADFA NR A+FDGSIS Q RQRFRRMWRVREHMAF
Sbjct: 541 QYLLRLKRTQMELEKSWASLMHQDHADFANNRNAQFDGSISLQRRQRFRRMWRVREHMAF 600

Query: 601 LIRNLQFYIQVDVIESQWNILQDHIQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRIL 660
           LIRNLQFYIQVDVIESQWNILQDHIQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRIL
Sbjct: 601 LIRNLQFYIQVDVIESQWNILQDHIQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRIL 660

Query: 661 DGIMKLCLQFCWSIENQDSNSDPPELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFLRR 720
           DGIMKLCLQFCWSIENQDS+SDP ELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFLRR
Sbjct: 661 DGIMKLCLQFCWSIENQDSSSDPSELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFLRR 720

Query: 721 FLMRLNFNSFFEATARGVLNVVRPRPAALPVLNQQ 754
           FLMRLNFNSFFEATARGVLNVVRPRPAALP+LNQQ
Sbjct: 721 FLMRLNFNSFFEATARGVLNVVRPRPAALPILNQQ 755

BLAST of Lag0031011 vs. ExPASy Swiss-Prot
Match: Q9SC88 (Gamma-tubulin complex component 4 homolog OS=Medicago truncatula OX=3880 GN=85P PE=2 SV=1)

HSP 1 Score: 1073.5 bits (2775), Expect = 7.6e-313
Identity = 560/758 (73.88%), Postives = 637/758 (84.04%), Query Frame = 0

Query: 1   MLHELLLALLGYTGDLIIDEREHYNSLGLNHLTPDAPISGEPTFKLAPDISFLEPSERDL 60
           MLHELLLALLGYTGDLIID R+       N+L+ + PIS E TFKLAPDISF++PS+R+L
Sbjct: 1   MLHELLLALLGYTGDLIIDRRD-------NNLSANTPISDECTFKLAPDISFIDPSDREL 60

Query: 61  IQRIIVLGFYYRELDRFATKSRNLSWIRSGNGSLLANSAESSKEKTENPSVYRRAIANGI 120
           I+RII LGFYYREL+RF+ KSRNL+WIRS N + L N         E PSVYRRA+ANGI
Sbjct: 61  IERIITLGFYYRELERFSAKSRNLNWIRSENANPLENK--------EKPSVYRRALANGI 120

Query: 121 VEILSVYRSAVLHVEQKLLSETVPILAVVTQGLDKFFVLFPPLHQLILEIERDDIRGGQL 180
           VEIL+VY S++LH+EQ LLSET+PILA VTQGL+KFF L PPL++LIL+IER DIRGG+L
Sbjct: 121 VEILAVYSSSILHIEQLLLSETMPILATVTQGLNKFFSLLPPLYELILKIERGDIRGGEL 180

Query: 181 LNLLHKRCHCGVPELQTCIQRLLWHGHQVLYNQLASWMVYGILQDKHGEFFIRRQDDRET 240
           LNLLHK+CHCGVPELQTCIQRLLWHGHQV+YNQLASWMVYGIL+D+HGEFFI RQ+ R+ 
Sbjct: 181 LNLLHKKCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYGILEDRHGEFFISRQEGRDV 240

Query: 241 EQGSSVQDMSEKLGRLST-DESLTDWHLGFHIYLDMLPDYIHMRVAEAILFAGKAIRVLR 300
           E  SS Q++SEKL RLST D SL+DWH+GFHI LDMLP+YI MRVAE+ILFAGKA+RVLR
Sbjct: 241 ENSSSHQEISEKLSRLSTADASLSDWHMGFHISLDMLPEYIPMRVAESILFAGKAVRVLR 300

Query: 301 NPSNAFWCQGTGNQSHSQIPRLPLNIKGNTRNFPLQKEPFVATKLTGEELLLQSEADKIE 360
           NPS +F  Q   +  + Q P+    I G    F  Q+EP + T +  E+LL QSEADKIE
Sbjct: 301 NPSPSFLSQ---DDVYPQEPKRFPKIHGFEGRFNFQREPIINTGMRVEDLLPQSEADKIE 360

Query: 361 AMLLDLKESSEFHKRSFESAVDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDF 420
            MLLDLKESSEFHKRSFE AVDSI+AIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDF
Sbjct: 361 NMLLDLKESSEFHKRSFECAVDSIQAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDF 420

Query: 421 FQCFLEESRHLMRLPPRQSTAEADLMIPFQLAATKTTSEEDKYFSRVSLRMPSFGVKVKS 480
           FQCFLEESR LMRLPPRQSTAEADLM+PFQLA+ KT  EEDKYFS+VSLRMPS+G+ VK 
Sbjct: 421 FQCFLEESRQLMRLPPRQSTAEADLMVPFQLASLKTIGEEDKYFSKVSLRMPSYGITVKP 480

Query: 481 A--QGDSKEKSYTDGNLGGALSNLSSDMSLDGWDGVALEYYIDWPLQLFFTQEVLSKYCR 540
           +         +  DG  G ++SN SS+MS+DGWDG+ALEY I+WPL LFFTQEVLS+Y +
Sbjct: 481 SLLNVPKATSAAADGISGASISNASSEMSVDGWDGIALEYSIEWPLHLFFTQEVLSRYLK 540

Query: 541 VFQYLLRLKRTQMELEKSWASLMHQDHADFAKNRRARFDGS-ISWQR-QRFRRMWRVREH 600
           VFQYLLRLKRTQMELEK WAS+MHQ H+ FAKN+++  D S I+ QR QRFR MWRVREH
Sbjct: 541 VFQYLLRLKRTQMELEKLWASVMHQYHSIFAKNKKSDQDKSPITQQRDQRFRSMWRVREH 600

Query: 601 MAFLIRNLQFYIQVDVIESQWNILQDHIQDSHDFTELVGFHQEYLSALISQSFLDIGSVS 660
           MAFLIRNLQFYIQVDVIESQWNILQ HIQDSHDFTELVGFHQEYLSALISQ+FLDIGSVS
Sbjct: 601 MAFLIRNLQFYIQVDVIESQWNILQSHIQDSHDFTELVGFHQEYLSALISQTFLDIGSVS 660

Query: 661 RILDGIMKLCLQFCWSIENQDSNSDPPELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPF 720
           RILDGIMKLCLQFCW+IENQD+ S+  ELEH+ EEFNKKSNSLYTILRSSRL GSQR PF
Sbjct: 661 RILDGIMKLCLQFCWNIENQDNFSNTSELEHIAEEFNKKSNSLYTILRSSRLAGSQRTPF 720

Query: 721 LRRFLMRLNFNSFFEATARGVLNVVRPRPAALPVLNQQ 754
           LRRFL+RLN NSFFE+TA+ V+NVVRPRP   P LNQ+
Sbjct: 721 LRRFLLRLNLNSFFESTAKEVMNVVRPRP-TFPGLNQR 739

BLAST of Lag0031011 vs. ExPASy Swiss-Prot
Match: Q9M350 (Gamma-tubulin complex component 4 OS=Arabidopsis thaliana OX=3702 GN=GCP4 PE=2 SV=2)

HSP 1 Score: 1065.1 bits (2753), Expect = 3.7e-310
Identity = 562/749 (75.03%), Postives = 630/749 (84.11%), Query Frame = 0

Query: 1   MLHELLLALLGYTGDLIIDEREHYNSLGLNHLTPDAPISGEPTFKLAPDISFLEPSERDL 60
           MLHELLLALLG+TGDLI+DERE   +LGL     D+P+S E TFKLAPDISF+EPSERDL
Sbjct: 1   MLHELLLALLGFTGDLIVDEREQRKTLGL-AFNSDSPLSDECTFKLAPDISFIEPSERDL 60

Query: 61  IQRIIVLGFYYRELDRFATKSRNLSWIRSGNG-SLLANSAESSKEKTE-NPSVYRRAIAN 120
           I+R+I LGFYYRELDRFA KSRNLSWIRS      L  + E SK+  E  PSVYRRAIAN
Sbjct: 61  IERLIKLGFYYRELDRFAKKSRNLSWIRSVTSVHPLERADELSKQSREKKPSVYRRAIAN 120

Query: 121 GIVEILSVYRSAVLHVEQKLLSETVPILAVVTQGLDKFFVLFPPLHQLILEIERDDIRGG 180
           GI EILSVYRSAVLH+EQKLL+ET PILA VT+GL+KFFVLFPPL+++ILEIERDDIRGG
Sbjct: 121 GIGEILSVYRSAVLHIEQKLLAETTPILATVTEGLNKFFVLFPPLYEVILEIERDDIRGG 180

Query: 181 QLLNLLHKRCHCGVPELQTCIQRLLWHGHQVLYNQLASWMVYGILQDKHGEFFIRRQDDR 240
           QLLN+L+KRCHCGVPEL+TC+QRLLW+GHQV+YNQLA+WMVYGILQD HGEFFI+RQDD 
Sbjct: 181 QLLNVLNKRCHCGVPELRTCLQRLLWNGHQVMYNQLAAWMVYGILQDPHGEFFIKRQDDG 240

Query: 241 ETEQGSSVQDMSEKLGRLSTDE-SLTDWHLGFHIYLDMLPDYIHMRVAEAILFAGKAIRV 300
           + +  SS +++SEKL R S  E SLTDWH GFHI LDMLPDYI MR+ E+ILFAGKAIRV
Sbjct: 241 DLDHRSSQEEVSEKLARTSVHETSLTDWHSGFHISLDMLPDYIPMRLGESILFAGKAIRV 300

Query: 301 LRNPSNAFWCQGTGNQSHSQIPRLPLNIKGNTRNFPLQKEPFVATKLTGEELLLQSEADK 360
           LRNPS AF  Q   ++S  Q  R    I+G   +   + E  +   LTG ELL QSEADK
Sbjct: 301 LRNPSPAF--QFQKDKSFQQTMRGSQRIRGFMHSDFPETETELDADLTGGELLPQSEADK 360

Query: 361 IEAMLLDLKESSEFHKRSFESAVDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKG 420
           IEAML DLKESSEFHKRSFE  VDS+RAIAASHLWQLVVVRADLNGHLKALKDYFLL KG
Sbjct: 361 IEAMLKDLKESSEFHKRSFECTVDSVRAIAASHLWQLVVVRADLNGHLKALKDYFLLEKG 420

Query: 421 DFFQCFLEESRHLMRLPPRQSTAEADLMIPFQLAATKTTSEEDKYFSRVSLRMPSFGVKV 480
           DFFQCFLEESR LMRLPPRQST E+DLM+PFQLAATKT +EEDKYFSRVSLRMPSFGV V
Sbjct: 421 DFFQCFLEESRQLMRLPPRQSTGESDLMVPFQLAATKTIAEEDKYFSRVSLRMPSFGVTV 480

Query: 481 KSAQGDSKEKSYTDGNLGGALSNLSSDMSLDGWDGVALEYYIDWPLQLFFTQEVLSKYCR 540
           +S+Q D         +L G  +NL+SD S+DGWD +ALEY +DWP+QLFFTQEVLSKY +
Sbjct: 481 RSSQADMVRSKV---SLTGK-ANLTSDTSVDGWDAIALEYSVDWPMQLFFTQEVLSKYLK 540

Query: 541 VFQYLLRLKRTQMELEKSWASLMHQDHADFAKNRRARFDGSISWQ-RQRFRRMWRVREHM 600
           VFQYL+RLKRTQMELEKSWAS+MHQDH + A++R+   +GS S Q RQ  R MWRVREHM
Sbjct: 541 VFQYLIRLKRTQMELEKSWASVMHQDHIESAQHRKDGLNGSTSQQRRQGIRPMWRVREHM 600

Query: 601 AFLIRNLQFYIQVDVIESQWNILQDHIQDSHDFTELVGFHQEYLSALISQSFLDIGSVSR 660
           AFLIRNLQFYIQVDVIESQW +LQ HI DS DFTELVGFHQEYLSALISQSFLDIGSVSR
Sbjct: 601 AFLIRNLQFYIQVDVIESQWKVLQTHIHDSQDFTELVGFHQEYLSALISQSFLDIGSVSR 660

Query: 661 ILDGIMKLCLQFCWSIENQDSNSDPPELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFL 720
           ILD IMKLCLQFCW+IENQ+SN +  ELE++ EEFNKKSNSLYTILRSS+L GSQRAPFL
Sbjct: 661 ILDSIMKLCLQFCWNIENQESNPNTSELENIAEEFNKKSNSLYTILRSSKLAGSQRAPFL 720

Query: 721 RRFLMRLNFNSFFEATARGVLNVVRPRPA 746
           RRFL+RLNFNSF+EATARGVLNVVR RPA
Sbjct: 721 RRFLLRLNFNSFYEATARGVLNVVRQRPA 742

BLAST of Lag0031011 vs. ExPASy Swiss-Prot
Match: Q9UGJ1 (Gamma-tubulin complex component 4 OS=Homo sapiens OX=9606 GN=TUBGCP4 PE=1 SV=1)

HSP 1 Score: 303.9 bits (777), Expect = 5.0e-81
Identity = 220/750 (29.33%), Postives = 363/750 (48.40%), Query Frame = 0

Query: 1   MLHELLLALLGYTGDLIIDEREHYNSLGLNHLTPDAPISGEPTFKLAPDISFLEPSERDL 60
           M+HELLLAL GY G +    +                 SG    +++ D  FL PSE  +
Sbjct: 1   MIHELLLALSGYPGSIFTWNKR----------------SG---LQVSQDFPFLHPSETSV 60

Query: 61  IQRIIVLGFYYRELDRFATKSRNLSWIRSGNGSLLANSAESSKEKTEN-PSVYRRAIANG 120
           + R+  LG  Y     F         I    G +       S++       +Y RA   G
Sbjct: 61  LNRLCRLGTDYIRFTEF---------IEQYTGHVQQQDHHPSQQGQGGLHGIYLRAFCTG 120

Query: 121 IVEILSVYRSAVLHVEQKLLSETVPILAVVTQGLDKFFVLFPPLHQLILEIERDDIRGGQ 180
           +  +L  YR A+L +EQ+ L +    ++ V   LD+F +LFP +  ++ +I+   I G Q
Sbjct: 121 LDSVLQPYRQALLDLEQEFLGDPHLSISHVNYFLDQFQLLFPSVMVVVEQIKSQKIHGCQ 180

Query: 181 LLNLLHKRCHCGVPELQTCIQRLLWHGHQVLYNQLASWMVYGILQDKHGEFFIRR----- 240
           +L  ++K    G+P +++ ++++L   H V+Y QL++WM++G+L D+H EFFI++     
Sbjct: 181 ILETVYKHSCGGLPPVRSALEKILAVCHGVMYKQLSAWMLHGLLLDQHEEFFIKQGPSSG 240

Query: 241 ----QDDRETEQ-------GSSVQDMSEKLGRLSTDESLTDWHLGFHIYLDMLPDYIHMR 300
               Q + + E        G  ++++ + L  +  +  L      F + +++LP YI +R
Sbjct: 241 NVSAQPEEDEEDLGIGGLTGKQLRELQD-LRLIEEENMLAPSLKQFSLRVEILPSYIPVR 300

Query: 301 VAEAILFAGKAIRVLRNPSNAFWCQGTGNQSHSQIPRLPLNIKGNTRNFPLQKEPFVATK 360
           VAE ILF G+++++  N +                                         
Sbjct: 301 VAEKILFVGESVQMFENQN---------------------------------------VN 360

Query: 361 LTGEELLLQSEADKIEAMLLDLKESSEFHKRSFESAVDSIRAIAASHLWQLVVVRADLNG 420
           LT +  +L+++ D   A L  LK+   F    FE  VD IR+  A HLW+L+V  +DL G
Sbjct: 361 LTRKGSILKNQEDTFAAELHRLKQQPLFSLVDFEQVVDRIRSTVAEHLWKLMVEESDLLG 420

Query: 421 HLKALKDYFLLAKGDFFQCFLEESRHLMRLPPRQSTAEADLMIPFQLAATKTTSEEDKYF 480
            LK +KD++LL +G+ FQ F++ ++H+++ PP   T E D+ + FQ +A K   ++D   
Sbjct: 421 QLKIIKDFYLLGRGELFQAFIDTAQHMLKTPPTAVT-EHDVNVAFQQSAHKVLLDDDNLL 480

Query: 481 SRVSLRMPSFGVKVKSAQGDSKEKSYTDGNLGGALSNLSSDMSLDGWDGVALEYYIDWPL 540
             + L +   G + K+    ++E        G +      +    GW  + L Y + WPL
Sbjct: 481 PLLHLTIEYHGKEHKADATQARE--------GPSRETSPREAPASGWAALGLSYKVQWPL 540

Query: 541 QLFFTQEVLSKYCRVFQYLLRLKRTQMELEKSWASLMHQDHADFAKNRRARFDGSISWQR 600
            + FT  VL KY  VF+YLL ++R Q EL+  WA  M + H                   
Sbjct: 541 HILFTPAVLEKYNVVFKYLLSVRRVQAELQHCWALQMQRKHLK---------------SN 600

Query: 601 QRFRRMWRVREHMAFLIRNLQFYIQVDVIESQWNILQDHIQDSHDFTELVGFHQEYLSAL 660
           Q     WR+R HMAFL+ NLQ+Y+QVDV+ESQ++ L   I  + DF  +   H  +LS L
Sbjct: 601 QTDAIKWRLRNHMAFLVDNLQYYLQVDVLESQFSQLLHQINSTRDFESIRLAHDHFLSNL 655

Query: 661 ISQSFLDIGSVSRILDGIMKLCLQFCWSIENQD----SNSDPPELEHLTEEFNKKSNSLY 720
           ++QSF+ +  V   L+ I+ LC  FC S+ +Q+          +L  L + F+++S+ L+
Sbjct: 661 LAQSFILLKPVFHCLNEILDLCHSFC-SLVSQNLGPLDERGAAQLSILVKGFSRQSSLLF 655

Query: 721 TILRSSRLVGSQRAPFLRRFLMRLNFNSFF 730
            IL S R    Q    L + L+RL++N ++
Sbjct: 721 KILSSVR--NHQINSDLAQLLLRLDYNKYY 655

BLAST of Lag0031011 vs. ExPASy Swiss-Prot
Match: Q9D4F8 (Gamma-tubulin complex component 4 OS=Mus musculus OX=10090 GN=Tubgcp4 PE=1 SV=2)

HSP 1 Score: 301.2 bits (770), Expect = 3.3e-80
Identity = 223/750 (29.73%), Postives = 364/750 (48.53%), Query Frame = 0

Query: 1   MLHELLLALLGYTGDLIIDEREHYNSLGLNHLTPDAPISGEPTFKLAPDISFLEPSERDL 60
           M+HELLLAL GY G +    +                 SG    +++ D  FL PSE  +
Sbjct: 1   MIHELLLALSGYPGSIFTWNKR----------------SG---LQVSQDFPFLHPSETSV 60

Query: 61  IQRIIVLGFYYRELDRFATKSRNLSWIRSGNGSLLANSAESSKEKTEN-PSVYRRAIANG 120
           + R+  LG  Y     F         I    G +        ++       +Y RA   G
Sbjct: 61  LNRLCRLGTDYIRFTEF---------IEQYTGHVQQQDHHPPQQGQGGLHGIYLRAFCTG 120

Query: 121 IVEILSVYRSAVLHVEQKLLSETVPILAVVTQGLDKFFVLFPPLHQLILEIERDDIRGGQ 180
           +  +L  YR A+L +EQ+ L++    ++ V   LD+F +LFP +  ++ +I+   I G Q
Sbjct: 121 LDSVLQPYRQALLDLEQEFLADPHLSISHVNYSLDQFQLLFPSVMVVVEQIKSQKIHGCQ 180

Query: 181 LLNLLHKRCHCGVPELQTCIQRLLWHGHQVLYNQLASWMVYGILQDKHGEFFIRRQDDRE 240
           +L  ++K    G+P +++ ++++L   H V+Y QL++WM++G+L D+H EFFI++     
Sbjct: 181 ILETVYKHSCGGLPPVRSALEKILAVCHGVMYKQLSAWMLHGLLLDQHEEFFIKQGPSSG 240

Query: 241 TEQGSSVQDMSEKLG---------------RLSTDESLTDWHL-GFHIYLDMLPDYIHMR 300
           T   + +++  E LG               RL  +E++    L  F + +++LP YI +R
Sbjct: 241 T-LSAQLEEDEEDLGIGGLTGKQLRELQDLRLIEEENMLAPSLKQFSLRVEILPSYIPVR 300

Query: 301 VAEAILFAGKAIRVLRNPSNAFWCQGTGNQSHSQIPRLPLNIKGNTRNFPLQKEPFVATK 360
           VAE ILF G+++++  N +                                         
Sbjct: 301 VAEKILFVGESVQMFENQN---------------------------------------VN 360

Query: 361 LTGEELLLQSEADKIEAMLLDLKESSEFHKRSFESAVDSIRAIAASHLWQLVVVRADLNG 420
           LT +  +L+++ D   A L  LK+   F    FE  VD IR+  A HLW+L+V  +DL G
Sbjct: 361 LTRKGSILKNQEDTFAAELHRLKQQPLFSLVDFEQVVDRIRSTVAEHLWKLMVEESDLLG 420

Query: 421 HLKALKDYFLLAKGDFFQCFLEESRHLMRLPPRQSTAEADLMIPFQLAATKTTSEEDKYF 480
            LK +KD++LL +G+ FQ F++ ++H+++ PP   T E D+ + FQ +A K   ++D   
Sbjct: 421 QLKIIKDFYLLGRGELFQAFIDTAQHMLKTPPTAVT-EHDVNVAFQQSAHKVLLDDDNLL 480

Query: 481 SRVSLRMPSFGVKVKSAQGDSKEKSYTDGNLGGALSNLSSDMSLDGWDGVALEYYIDWPL 540
             + L +   G   K+     +E    + +   A S+        GW  + L Y + WPL
Sbjct: 481 PLLHLTIEYHGKDHKADATQPREVPSRETSPREAPSS--------GWAALGLSYKVQWPL 540

Query: 541 QLFFTQEVLSKYCRVFQYLLRLKRTQMELEKSWASLMHQDHADFAKNRRARFDGSISWQR 600
            + FT  VL KY  VF+YLL ++R Q EL+  WA  M + H                   
Sbjct: 541 HILFTPAVLEKYNVVFKYLLSVRRVQAELQHCWALQMQRKHLK---------------SN 600

Query: 601 QRFRRMWRVREHMAFLIRNLQFYIQVDVIESQWNILQDHIQDSHDFTELVGFHQEYLSAL 660
           Q     WR+R HMAFL+ NLQ+Y+QVDV+ESQ++ L   I  + DF  +   H  +LS L
Sbjct: 601 QTDAVKWRLRNHMAFLVDNLQYYLQVDVLESQFSQLLHQINSTRDFESIRLAHDHFLSNL 655

Query: 661 ISQSFLDIGSVSRILDGIMKLCLQFCWSIENQD----SNSDPPELEHLTEEFNKKSNSLY 720
           ++QSF+ +  V   L+ I+ LC  FC S+ +Q+          +L  L + F+++S+ L+
Sbjct: 661 LAQSFILLKPVFHCLNEILDLCHSFC-SLVSQNLGPLDERGAAQLSILVKGFSRQSSLLF 655

Query: 721 TILRSSRLVGSQRAPFLRRFLMRLNFNSFF 730
            IL S R    Q    L + L+RL++N ++
Sbjct: 721 KILSSVR--NHQINSDLAQLLLRLDYNKYY 655

BLAST of Lag0031011 vs. ExPASy Swiss-Prot
Match: Q9VKU7 (Gamma-tubulin complex component 4 homolog OS=Drosophila melanogaster OX=7227 GN=Grip75 PE=1 SV=2)

HSP 1 Score: 167.2 bits (422), Expect = 7.3e-40
Identity = 172/703 (24.47%), Postives = 311/703 (44.24%), Query Frame = 0

Query: 52  FLEPSERDLIQRIIVLGFYYRELDRFATKSRNLSWIRSGNGSLLANSAESSKEKTENPSV 111
           F+ P ER++   II +   Y+E+++F   S      +S     L +S             
Sbjct: 31  FIHPCEREIFMDIIKIIKVYQEVEQFTHSSGR----KSDTHGELPDSLH---------GY 90

Query: 112 YRRAIANGIVEILSVYRSAVLHVEQKLLSETVPILAVVTQGLDKFFVLFPPLHQLILEIE 171
           Y   +A GI   L  Y + +  +E+  L      L+ V   L   F L   +  LI EI 
Sbjct: 91  YLLNLAKGIEMALEEYYAEIGRLEKYCLGNERNSLSYVYNALYAKFPLLVFMRNLITEIH 150

Query: 172 RDDIRGGQLLNLLHKRCHCGVPELQTCIQRLLWHGHQVLYNQLASWMVYGILQDKHGEFF 231
             ++RG  LL+ LH++C  G  +L+  I+ ++       ++ LA W+++G++ D H EFF
Sbjct: 151 VLNLRGCVLLHNLHQQCEHGDIQLEKAIKIIMKPVKNAFFSSLAHWLLFGVIDDVHSEFF 210

Query: 232 IRRQDDRETEQGSSVQDMSEKLGRLSTDESLTDWHLGFHIYLDMLPDYIHMRVAEAILFA 291
           I +    +   GSS    S     LS +++  D+   + + +  LP +  + +AE +LF 
Sbjct: 211 I-KFTPTDAVDGSSF-SKSATCSLLSAEKNPEDYIWQYEVNMSQLPGFFSIVLAEKVLFV 270

Query: 292 GKAIRVLRNPSNAFWCQGTGNQSHSQIPRLPLNIKGNTRNFPLQKEPFVATKLTGEEL-- 351
           G+ + V                      ++  N+K   +  PL  +     +L  +++  
Sbjct: 271 GQTVLVF---------------------KMGRNVKVKNKTDPLAAK---LAELDSDDIYQ 330

Query: 352 LLQSEADKIEAMLLDLKESSEFHKRSFESAVDSIRAIAASHLWQLVVVRADLNGHLKALK 411
           L      +   M++DL      +    E  +  I+   ++ L ++ V   DL   +  +K
Sbjct: 331 LWSGRESEFFKMVVDLSNEDTINVFRLEKVIIDIKNYVSARLSEIAVNEVDLERQMGLIK 390

Query: 412 DYFLLAKGDFFQCFLEESRHLMRLPPRQSTAEADLMIPFQLAATKT--TSEEDKYFSRVS 471
           D+FLL +G+F+  F   S+ +  +   +     ++   F+LAAT T  T + DK FS + 
Sbjct: 391 DFFLLGRGEFYLEFC--SQMVGTMETYREERFKNVTRSFELAATVTGITDDLDK-FSLIC 450

Query: 472 LRMPSFGVKVKSAQGDSKEKSYTDGNLGGALSNLSSDMSLDGWDGVALEYYIDWPLQLFF 531
            R              S+    +D N                  G++L+Y  +WPL L F
Sbjct: 451 QR------------STSEPDDTSDFNF---------------LQGLSLKYEYEWPLNLLF 510

Query: 532 TQEVLSKYCRVFQYLLRLKRTQMELEKSWASLMHQDHADFAKNRRARFDGSISWQRQRFR 591
           +   + +Y  +F++LL ++  Q E+++ WA           +  RA+    +        
Sbjct: 511 SPTTIERYNNIFRFLLIIRTYQYEIQRVWAK----------QTWRAKSAKDVPPN----N 570

Query: 592 RMWRVREHMAFLIRNLQFYIQVDVIESQWNILQDHIQDSHDFTELVGFHQEYLSALISQS 651
           ++  +R ++ F + N+Q+YIQVDV+ESQ+ IL + I+   DF  +   H  +L+ ++S  
Sbjct: 571 KIITLRNYLMFFLNNMQYYIQVDVLESQFGILMNVIKSRSDFEVIQRAHTVFLANVLSHC 630

Query: 652 FL---------DIGSVSR--ILDGIMKL---CLQFCWSIENQDSNSD-PPELEHLTEEFN 711
           FL           GS +R  I   ++KL   C +F    + +D + D   E++ L E F 
Sbjct: 631 FLLNESETQLNVTGSQNRNPIYGTLLKLFGICEKFAHMTQTKDPSDDLEDEVDQLNESFG 645

Query: 712 KKSNSLYTIL---RSSRLVGSQRAPFLRRFLMRLNFNSFFEAT 733
            +  SL  +L   +S+  +G      L + L+RL+FN +F A+
Sbjct: 691 VQIASLIQLLVDVKSASCLGP-----LSQLLLRLDFNCWFSAS 645

BLAST of Lag0031011 vs. ExPASy TrEMBL
Match: A0A6J1F2V2 (Gamma-tubulin complex component OS=Cucurbita moschata OX=3662 GN=LOC111441869 PE=3 SV=1)

HSP 1 Score: 1416.7 bits (3666), Expect = 0.0e+00
Identity = 714/754 (94.69%), Postives = 731/754 (96.95%), Query Frame = 0

Query: 1   MLHELLLALLGYTGDLIIDEREHYNSLGLNHLTPDAPISGEPTFKLAPDISFLEPSERDL 60
           MLHELLLALLGYTGDLIIDEREHYN LGLNHL PDAPISGEPTFKLAPDISFLEPSERDL
Sbjct: 1   MLHELLLALLGYTGDLIIDEREHYNLLGLNHLPPDAPISGEPTFKLAPDISFLEPSERDL 60

Query: 61  IQRIIVLGFYYRELDRFATKSRNLSWIRSGNGSLLANSAESSKEKTENPSVYRRAIANGI 120
           IQRIIVLGFYYRELDRFATKSRNLSWIRS NGS L NS ESSK+KTE PSVYRRAIANGI
Sbjct: 61  IQRIIVLGFYYRELDRFATKSRNLSWIRSSNGSSLPNSVESSKDKTEIPSVYRRAIANGI 120

Query: 121 VEILSVYRSAVLHVEQKLLSETVPILAVVTQGLDKFFVLFPPLHQLILEIERDDIRGGQL 180
           VEILSVYRSAVLHVEQKLLSETVPILA+VTQGLDKFFVLFPPLHQL+LEIERDDIRGGQL
Sbjct: 121 VEILSVYRSAVLHVEQKLLSETVPILAIVTQGLDKFFVLFPPLHQLVLEIERDDIRGGQL 180

Query: 181 LNLLHKRCHCGVPELQTCIQRLLWHGHQVLYNQLASWMVYGILQDKHGEFFIRRQDDRET 240
           LNLLHKRCHCGVPELQTCIQRLLWHGHQV+YNQLASWMVYGILQDKHGEFFIRRQD RET
Sbjct: 181 LNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYGILQDKHGEFFIRRQDGRET 240

Query: 241 EQGSSVQDMSEKLGRLSTDESLTDWHLGFHIYLDMLPDYIHMRVAEAILFAGKAIRVLRN 300
           +QGS+VQ +SEKLGRLSTDESLTDWHLGFHIYLDMLPDYIHM+VAE+ILFAGKAIRVLRN
Sbjct: 241 DQGSAVQVLSEKLGRLSTDESLTDWHLGFHIYLDMLPDYIHMKVAESILFAGKAIRVLRN 300

Query: 301 PSNAFWCQGTGNQSHSQIPRLPLNIKGNTRNFPLQKEPFVATKLTGEELLLQSEADKIEA 360
           PSNAFWCQG GNQSHS +PRLPL IKGNTRNFPLQKEPF ATKLTGEELLLQSEADKIEA
Sbjct: 301 PSNAFWCQGAGNQSHSHMPRLPLKIKGNTRNFPLQKEPFFATKLTGEELLLQSEADKIEA 360

Query: 361 MLLDLKESSEFHKRSFESAVDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDFF 420
           MLLDLKESSEFHKRSFESA+DSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDFF
Sbjct: 361 MLLDLKESSEFHKRSFESAIDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDFF 420

Query: 421 QCFLEESRHLMRLPPRQSTAEADLMIPFQLAATKTTSEEDKYFSRVSLRMPSFGVKVKSA 480
           QCFLEESRHLMRLPPRQSTAEADLMIPFQLAATKTTS+ED+YFSRVSLRMPSFGVKVKSA
Sbjct: 421 QCFLEESRHLMRLPPRQSTAEADLMIPFQLAATKTTSDEDRYFSRVSLRMPSFGVKVKSA 480

Query: 481 QGDSKEKSYTDGNLGGALSNLSSDMSLDGWDGVALEYYIDWPLQLFFTQEVLSKYCRVFQ 540
           QGD KEK+YTDGN GGALSNLSSDMSLDGWD VALEYYIDWPLQLFFTQEVLSKYCRVFQ
Sbjct: 481 QGDPKEKTYTDGNPGGALSNLSSDMSLDGWDSVALEYYIDWPLQLFFTQEVLSKYCRVFQ 540

Query: 541 YLLRLKRTQMELEKSWASLMHQDHADFAKNRRARFDGSISWQ-RQRFRRMWRVREHMAFL 600
           YLLRLKRTQMELEKSWASLMHQDHADF+KNR ARFDGSIS Q RQRFR+MWRVREHMAFL
Sbjct: 541 YLLRLKRTQMELEKSWASLMHQDHADFSKNRNARFDGSISPQKRQRFRQMWRVREHMAFL 600

Query: 601 IRNLQFYIQVDVIESQWNILQDHIQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRILD 660
           IRNLQFYIQVDVIESQWNILQD IQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRILD
Sbjct: 601 IRNLQFYIQVDVIESQWNILQDRIQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRILD 660

Query: 661 GIMKLCLQFCWSIENQDSNSDPPELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFLRRF 720
           GIMKLCLQFCWSIENQD +SDP ELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFLRRF
Sbjct: 661 GIMKLCLQFCWSIENQDKSSDPSELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFLRRF 720

Query: 721 LMRLNFNSFFEATARGVLNVVRPRPAALPVLNQQ 754
           LMRLNFNSFFEATARGVLNVV PRPAALPVLNQQ
Sbjct: 721 LMRLNFNSFFEATARGVLNVVGPRPAALPVLNQQ 754

BLAST of Lag0031011 vs. ExPASy TrEMBL
Match: A0A5A7SW23 (Gamma-tubulin complex component OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold228G00060 PE=3 SV=1)

HSP 1 Score: 1414.1 bits (3659), Expect = 0.0e+00
Identity = 716/755 (94.83%), Postives = 731/755 (96.82%), Query Frame = 0

Query: 1   MLHELLLALLGYTGDLIIDEREHYNSLGLNHLTPDAPISGEPTFKLAPDISFLEPSERDL 60
           MLHELLLALLGYTGDLIIDEREHYNSLGLN+L  DAPISGEPTFKLA DISFLEPSERDL
Sbjct: 1   MLHELLLALLGYTGDLIIDEREHYNSLGLNNLLVDAPISGEPTFKLASDISFLEPSERDL 60

Query: 61  IQRIIVLGFYYRELDRFATKSRNLSWIRSGNGSLLANSAESSKEKTENPSVYRRAIANGI 120
           IQRIIVLGFYYRELDRFATKSRNLSWIRSGN S LANS ESSK+K ENPSVYRRAIANGI
Sbjct: 61  IQRIIVLGFYYRELDRFATKSRNLSWIRSGNESSLANSTESSKDKIENPSVYRRAIANGI 120

Query: 121 VEILSVYRSAVLHVEQKLLSETVPILAVVTQGLDKFFVLFPPLHQLILEIERDDIRGGQL 180
           VEILS+YRSAVLHVEQKLLSETVPILA+VTQGLDKFFVLFPPLHQLI EIERDDI+GGQL
Sbjct: 121 VEILSIYRSAVLHVEQKLLSETVPILAIVTQGLDKFFVLFPPLHQLIREIERDDIQGGQL 180

Query: 181 LNLLHKRCHCGVPELQTCIQRLLWHGHQVLYNQLASWMVYGILQDKHGEFFIRRQDDRET 240
           LNLLHKRCHCGVPELQTCIQRLLWHGHQV+YNQLASWMVYG+LQDKHGEFFIRRQDDRET
Sbjct: 181 LNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYGLLQDKHGEFFIRRQDDRET 240

Query: 241 EQGSSVQDMSEKLGRLSTDESLTDWHLGFHIYLDMLPDYIHMRVAEAILFAGKAIRVLRN 300
            QGSSVQDMSEKLGRLSTDESLTDWHLGFHIYLDMLPDYIHMRVAE+ILFAGKAIRVLRN
Sbjct: 241 NQGSSVQDMSEKLGRLSTDESLTDWHLGFHIYLDMLPDYIHMRVAESILFAGKAIRVLRN 300

Query: 301 PSNAFWCQGTGNQSHSQIPRLPLNIKGNTRNFPLQKEPFVATKLTGEELLLQSEADKIEA 360
           PSNAFWCQ  GNQSHS +PRLPLN+KGNTRNFPLQKEPFVATKLTGEELLLQSEADKIEA
Sbjct: 301 PSNAFWCQSAGNQSHSNMPRLPLNVKGNTRNFPLQKEPFVATKLTGEELLLQSEADKIEA 360

Query: 361 MLLDLKESSEFHKRSFESAVDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDFF 420
           MLLDLKESSEFHKRSFESAVDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDFF
Sbjct: 361 MLLDLKESSEFHKRSFESAVDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDFF 420

Query: 421 QCFLEESRHLMRLPPRQSTAEADLMIPFQLAATKTTSEEDKYFSRVSLRMPSFGVKVKSA 480
           QCFLEESRHLMRLPPRQSTAEADLMIPFQLAATKTTSEED+YFSRVSLRMPSFGVKVKSA
Sbjct: 421 QCFLEESRHLMRLPPRQSTAEADLMIPFQLAATKTTSEEDRYFSRVSLRMPSFGVKVKSA 480

Query: 481 QGDSKEKSYTDGNLGGAL-SNLSSDMSLDGWDGVALEYYIDWPLQLFFTQEVLSKYCRVF 540
           QGD KEK   DGN GGAL SNLS DMSLDGWDGV+LEYYIDWPLQLFFTQEVLSKYCRVF
Sbjct: 481 QGDLKEKPIIDGNPGGALSSNLSLDMSLDGWDGVSLEYYIDWPLQLFFTQEVLSKYCRVF 540

Query: 541 QYLLRLKRTQMELEKSWASLMHQDHADFAKNRRARFDGSISWQ-RQRFRRMWRVREHMAF 600
           QYLLRLKRTQMELEKSWASLMHQDHADFA NR A+FDGSIS Q RQRFRRMWRVREHMAF
Sbjct: 541 QYLLRLKRTQMELEKSWASLMHQDHADFANNRNAQFDGSISLQRRQRFRRMWRVREHMAF 600

Query: 601 LIRNLQFYIQVDVIESQWNILQDHIQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRIL 660
           LIRNLQFYIQVDVIESQWNILQDHIQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRIL
Sbjct: 601 LIRNLQFYIQVDVIESQWNILQDHIQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRIL 660

Query: 661 DGIMKLCLQFCWSIENQDSNSDPPELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFLRR 720
           DGIMKLCLQFCWSIENQDS+SDP ELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFLRR
Sbjct: 661 DGIMKLCLQFCWSIENQDSSSDPSELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFLRR 720

Query: 721 FLMRLNFNSFFEATARGVLNVVRPRPAALPVLNQQ 754
           FLMRLNFNSFFEATARGVLNVVRPRPAALP+LNQQ
Sbjct: 721 FLMRLNFNSFFEATARGVLNVVRPRPAALPILNQQ 755

BLAST of Lag0031011 vs. ExPASy TrEMBL
Match: A0A6J1DP06 (Gamma-tubulin complex component OS=Momordica charantia OX=3673 GN=LOC111022444 PE=3 SV=1)

HSP 1 Score: 1406.3 bits (3639), Expect = 0.0e+00
Identity = 712/754 (94.43%), Postives = 730/754 (96.82%), Query Frame = 0

Query: 1   MLHELLLALLGYTGDLIIDEREHYNSLGLNHLTPDAPISGEPTFKLAPDISFLEPSERDL 60
           MLHELLLALLGYTGDLIIDEREHYNSLGLNHL PDAPISGE TFKLA DISFLEPSERDL
Sbjct: 1   MLHELLLALLGYTGDLIIDEREHYNSLGLNHLPPDAPISGESTFKLASDISFLEPSERDL 60

Query: 61  IQRIIVLGFYYRELDRFATKSRNLSWIRSGNGSLLANSAESSKEKTENPSVYRRAIANGI 120
           IQRIIVLGFYYRELDRFATKSRNLSWIRSGNGS LA SAESSK KTENPSVYRRAIANGI
Sbjct: 61  IQRIIVLGFYYRELDRFATKSRNLSWIRSGNGSSLAKSAESSKAKTENPSVYRRAIANGI 120

Query: 121 VEILSVYRSAVLHVEQKLLSETVPILAVVTQGLDKFFVLFPPLHQLILEIERDDIRGGQL 180
           VEILS+YRSAVLHVEQKLLSETVPILA+VTQGLDKFFVLFPPLHQL+LEIERDDIRGGQL
Sbjct: 121 VEILSIYRSAVLHVEQKLLSETVPILAIVTQGLDKFFVLFPPLHQLVLEIERDDIRGGQL 180

Query: 181 LNLLHKRCHCGVPELQTCIQRLLWHGHQVLYNQLASWMVYGILQDKHGEFFIRRQDDRET 240
           LNLLHKRCHCGVPELQTCIQRLLWHGHQV+YNQLASWMVYGILQD HGEFFIRRQDDRET
Sbjct: 181 LNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYGILQDNHGEFFIRRQDDRET 240

Query: 241 EQGSSVQDMSEKLGRLSTDESLTDWHLGFHIYLDMLPDYIHMRVAEAILFAGKAIRVLRN 300
           EQGSSVQDMSEKLGRLSTD+SLTDWHLGFHIYLDMLPDYIHM VAE+ILFAGKAIRVLRN
Sbjct: 241 EQGSSVQDMSEKLGRLSTDDSLTDWHLGFHIYLDMLPDYIHMWVAESILFAGKAIRVLRN 300

Query: 301 PSNAFWCQGTGNQSHSQIPRLPLNIKGNTRNFPLQKEPFVATKLTGEELLLQSEADKIEA 360
           PSNAFWCQG G QSHS I R+PLNIKGNTRNFPLQKEPF+ATKLTGEELLLQSEADKIEA
Sbjct: 301 PSNAFWCQGAGYQSHSNISRVPLNIKGNTRNFPLQKEPFIATKLTGEELLLQSEADKIEA 360

Query: 361 MLLDLKESSEFHKRSFESAVDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDFF 420
           MLLDLKE+SEFHKRSFESAVDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDFF
Sbjct: 361 MLLDLKEASEFHKRSFESAVDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDFF 420

Query: 421 QCFLEESRHLMRLPPRQSTAEADLMIPFQLAATKTTSEEDKYFSRVSLRMPSFGVKVKSA 480
           QCFLEESRHLMRLPPRQSTAEADLMIPFQLAATKTTSEEDKYFSRVSLRMPSFGV+VKS 
Sbjct: 421 QCFLEESRHLMRLPPRQSTAEADLMIPFQLAATKTTSEEDKYFSRVSLRMPSFGVRVKST 480

Query: 481 QGDSKEKSYTDGNLGGALSNLSSDMSLDGWDGVALEYYIDWPLQLFFTQEVLSKYCRVFQ 540
           Q    EK+YTDGN G ALSNLSSDMSLDGWDGVALEYYIDWPLQLFFTQEVLSKYCRVFQ
Sbjct: 481 Q----EKTYTDGNPGAALSNLSSDMSLDGWDGVALEYYIDWPLQLFFTQEVLSKYCRVFQ 540

Query: 541 YLLRLKRTQMELEKSWASLMHQDHADFAKNRRARFDGSI-SWQRQRFRRMWRVREHMAFL 600
           YLLRLKRTQMELEKSWASLMHQDHADFA+NRRA+ DGS+ S QRQRFRRMWRVREHMAFL
Sbjct: 541 YLLRLKRTQMELEKSWASLMHQDHADFAQNRRAQRDGSVSSHQRQRFRRMWRVREHMAFL 600

Query: 601 IRNLQFYIQVDVIESQWNILQDHIQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRILD 660
           IRNLQFYIQVDVIESQWNILQDHIQDSHDFT+LVG+HQEYLSALISQSFLDIGSVSRILD
Sbjct: 601 IRNLQFYIQVDVIESQWNILQDHIQDSHDFTDLVGYHQEYLSALISQSFLDIGSVSRILD 660

Query: 661 GIMKLCLQFCWSIENQDSNSDPPELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFLRRF 720
           GIMKLCLQFCWSIENQDS+S+P ELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFLRRF
Sbjct: 661 GIMKLCLQFCWSIENQDSSSNPSELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFLRRF 720

Query: 721 LMRLNFNSFFEATARGVLNVVRPRPAALPVLNQQ 754
           LMRLNFNSFFEATARGVLNVVR RPAALPVLNQQ
Sbjct: 721 LMRLNFNSFFEATARGVLNVVRARPAALPVLNQQ 750

BLAST of Lag0031011 vs. ExPASy TrEMBL
Match: A0A5A7VFR5 (Gamma-tubulin complex component OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold418G00280 PE=3 SV=1)

HSP 1 Score: 1406.0 bits (3638), Expect = 0.0e+00
Identity = 714/755 (94.57%), Postives = 728/755 (96.42%), Query Frame = 0

Query: 1   MLHELLLALLGYTGDLIIDEREHYNSLGLNHLTPDAPISGEPTFKLAPDISFLEPSERDL 60
           MLHELLLALLGYTGDLIIDEREHYNSLGLN+L  DAPISGEPTFKLA DISFLEPSERDL
Sbjct: 1   MLHELLLALLGYTGDLIIDEREHYNSLGLNNLLVDAPISGEPTFKLASDISFLEPSERDL 60

Query: 61  IQRIIVLGFYYRELDRFATKSRNLSWIRSGNGSLLANSAESSKEKTENPSVYRRAIANGI 120
           IQRIIVLGFYYRELDRFATKSRNLSWIRSGN S LA S ESSK+K ENPSVYRRAIANGI
Sbjct: 61  IQRIIVLGFYYRELDRFATKSRNLSWIRSGNESSLAISTESSKDKIENPSVYRRAIANGI 120

Query: 121 VEILSVYRSAVLHVEQKLLSETVPILAVVTQGLDKFFVLFPPLHQLILEIERDDIRGGQL 180
           VEILS+YRSAVLHVEQKLLSETVPILA+VTQGLDKFFVLFPPLHQLI EIERDDIRGGQL
Sbjct: 121 VEILSIYRSAVLHVEQKLLSETVPILAIVTQGLDKFFVLFPPLHQLICEIERDDIRGGQL 180

Query: 181 LNLLHKRCHCGVPELQTCIQRLLWHGHQVLYNQLASWMVYGILQDKHGEFFIRRQDDRET 240
           LNLLHKRCHCGVPELQTCIQRLLWHGHQV+YNQLASWMVYG+LQDKHGEFFIRRQDDRET
Sbjct: 181 LNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYGLLQDKHGEFFIRRQDDRET 240

Query: 241 EQGSSVQDMSEKLGRLSTDESLTDWHLGFHIYLDMLPDYIHMRVAEAILFAGKAIRVLRN 300
            QGSSVQDMSEKLGRLSTDESLTDWHLGFHIYLDMLPDYIHMRVAE+ILFAGKAIRVLRN
Sbjct: 241 NQGSSVQDMSEKLGRLSTDESLTDWHLGFHIYLDMLPDYIHMRVAESILFAGKAIRVLRN 300

Query: 301 PSNAFWCQGTGNQSHSQIPRLPLNIKGNTRNFPLQKEPFVATKLTGEELLLQSEADKIEA 360
           PSNAFWCQG GNQSHS +PRLPLN+KGN RNFPLQKEPFVATKLTGEELLLQSEADKIEA
Sbjct: 301 PSNAFWCQGAGNQSHSNMPRLPLNVKGNARNFPLQKEPFVATKLTGEELLLQSEADKIEA 360

Query: 361 MLLDLKESSEFHKRSFESAVDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDFF 420
           MLLDLKESSEFHKRSFESAVDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDFF
Sbjct: 361 MLLDLKESSEFHKRSFESAVDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDFF 420

Query: 421 QCFLEESRHLMRLPPRQSTAEADLMIPFQLAATKTTSEEDKYFSRVSLRMPSFGVKVKSA 480
           QCFLEESRHLMRLPPRQSTAEADLMIPFQLAATKTTSEED+YFSRVSLRMPSFGVKVKSA
Sbjct: 421 QCFLEESRHLMRLPPRQSTAEADLMIPFQLAATKTTSEEDRYFSRVSLRMPSFGVKVKSA 480

Query: 481 QGDSKEKSYTDGNLGGAL-SNLSSDMSLDGWDGVALEYYIDWPLQLFFTQEVLSKYCRVF 540
           QGD KEK   DGN GGAL SNLS DMS DGWDGV+LEYYIDWPLQLFFTQEVLSKYCRVF
Sbjct: 481 QGDLKEKPIIDGNPGGALSSNLSLDMSPDGWDGVSLEYYIDWPLQLFFTQEVLSKYCRVF 540

Query: 541 QYLLRLKRTQMELEKSWASLMHQDHADFAKNRRARFDGSISWQ-RQRFRRMWRVREHMAF 600
           QYLLRLKRTQMELEKSWASLMHQDHADFA  R A+FDGSIS Q RQRFRRMWRVREHMAF
Sbjct: 541 QYLLRLKRTQMELEKSWASLMHQDHADFANIRNAQFDGSISLQRRQRFRRMWRVREHMAF 600

Query: 601 LIRNLQFYIQVDVIESQWNILQDHIQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRIL 660
           LIRNLQFYIQVDVIESQWNILQDHIQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRIL
Sbjct: 601 LIRNLQFYIQVDVIESQWNILQDHIQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRIL 660

Query: 661 DGIMKLCLQFCWSIENQDSNSDPPELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFLRR 720
           DGIMKLCLQFCWSIENQDS+SDP ELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFLRR
Sbjct: 661 DGIMKLCLQFCWSIENQDSSSDPSELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFLRR 720

Query: 721 FLMRLNFNSFFEATARGVLNVVRPRPAALPVLNQQ 754
           FLMRLNFNSFFEATARGVLNVVRPRP+ALPVLNQQ
Sbjct: 721 FLMRLNFNSFFEATARGVLNVVRPRPSALPVLNQQ 755

BLAST of Lag0031011 vs. ExPASy TrEMBL
Match: A0A6J1J9A7 (Gamma-tubulin complex component OS=Cucurbita maxima OX=3661 GN=LOC111482469 PE=3 SV=1)

HSP 1 Score: 1400.2 bits (3623), Expect = 0.0e+00
Identity = 706/754 (93.63%), Postives = 727/754 (96.42%), Query Frame = 0

Query: 1   MLHELLLALLGYTGDLIIDEREHYNSLGLNHLTPDAPISGEPTFKLAPDISFLEPSERDL 60
           MLHELLLALLGYTGDLIIDEREH+N LGLNHL PDAPISGEPTFKLAPDISFLE SERDL
Sbjct: 1   MLHELLLALLGYTGDLIIDEREHFNLLGLNHLPPDAPISGEPTFKLAPDISFLELSERDL 60

Query: 61  IQRIIVLGFYYRELDRFATKSRNLSWIRSGNGSLLANSAESSKEKTENPSVYRRAIANGI 120
           IQRI+VLGFYYRELDRFATKSRNLSWIRS +GS L NS ESSK+KTE PSVYRRAIANGI
Sbjct: 61  IQRIVVLGFYYRELDRFATKSRNLSWIRSSHGSSLPNSVESSKDKTEIPSVYRRAIANGI 120

Query: 121 VEILSVYRSAVLHVEQKLLSETVPILAVVTQGLDKFFVLFPPLHQLILEIERDDIRGGQL 180
           VEILSVYRSAVLHVEQKLLSETVPILA+VTQGLDKFFVLFPPLHQL+LEIERDDIRGGQL
Sbjct: 121 VEILSVYRSAVLHVEQKLLSETVPILAIVTQGLDKFFVLFPPLHQLVLEIERDDIRGGQL 180

Query: 181 LNLLHKRCHCGVPELQTCIQRLLWHGHQVLYNQLASWMVYGILQDKHGEFFIRRQDDRET 240
           LNLLHKRCHCGVPELQTCIQRLLWHGHQV+YNQLASWMVYGILQDKHGEFFIRRQDDRET
Sbjct: 181 LNLLHKRCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYGILQDKHGEFFIRRQDDRET 240

Query: 241 EQGSSVQDMSEKLGRLSTDESLTDWHLGFHIYLDMLPDYIHMRVAEAILFAGKAIRVLRN 300
           +QGS+VQ +SEKLGRLSTDESLTDWHLGFHIYLDMLPDYIHM+VAE+ILFAGKAIRVLRN
Sbjct: 241 DQGSAVQVLSEKLGRLSTDESLTDWHLGFHIYLDMLPDYIHMKVAESILFAGKAIRVLRN 300

Query: 301 PSNAFWCQGTGNQSHSQIPRLPLNIKGNTRNFPLQKEPFVATKLTGEELLLQSEADKIEA 360
           PSNAFWCQG GNQSHS +PRLPL IKG TRNFPLQKEPF ATKLTGEELLLQSEADKIEA
Sbjct: 301 PSNAFWCQGAGNQSHSHMPRLPLKIKGTTRNFPLQKEPFFATKLTGEELLLQSEADKIEA 360

Query: 361 MLLDLKESSEFHKRSFESAVDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDFF 420
           MLLDLKESSEFHKRSFESA+DSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDFF
Sbjct: 361 MLLDLKESSEFHKRSFESAIDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDFF 420

Query: 421 QCFLEESRHLMRLPPRQSTAEADLMIPFQLAATKTTSEEDKYFSRVSLRMPSFGVKVKSA 480
           QCFLEES HLMRLPPRQSTAEADLMIPFQLAATKTTS+ED+YFSRVSLRMP FGVKVKSA
Sbjct: 421 QCFLEESCHLMRLPPRQSTAEADLMIPFQLAATKTTSDEDRYFSRVSLRMPLFGVKVKSA 480

Query: 481 QGDSKEKSYTDGNLGGALSNLSSDMSLDGWDGVALEYYIDWPLQLFFTQEVLSKYCRVFQ 540
           QGD KEK YTDGN GGALSNLSSDMSLDGW+GVALEYYIDWPLQLFFTQEVLSKYCRVFQ
Sbjct: 481 QGDPKEKPYTDGNPGGALSNLSSDMSLDGWNGVALEYYIDWPLQLFFTQEVLSKYCRVFQ 540

Query: 541 YLLRLKRTQMELEKSWASLMHQDHADFAKNRRARFDGSIS-WQRQRFRRMWRVREHMAFL 600
           YLLRLKRTQMELEKSWASLMHQDHADF+KNR ARFDGSIS  QRQ FR+MWRVREHMAFL
Sbjct: 541 YLLRLKRTQMELEKSWASLMHQDHADFSKNRNARFDGSISPQQRQHFRQMWRVREHMAFL 600

Query: 601 IRNLQFYIQVDVIESQWNILQDHIQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRILD 660
           IRNLQFYIQVDVIESQWNILQD IQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRILD
Sbjct: 601 IRNLQFYIQVDVIESQWNILQDRIQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRILD 660

Query: 661 GIMKLCLQFCWSIENQDSNSDPPELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFLRRF 720
           GIMKLCLQFCWSIENQ+ +SDP ELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFLRRF
Sbjct: 661 GIMKLCLQFCWSIENQNKSSDPSELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFLRRF 720

Query: 721 LMRLNFNSFFEATARGVLNVVRPRPAALPVLNQQ 754
           LMRLNFNSFFEATARGVLNVVR RPAALPVLNQQ
Sbjct: 721 LMRLNFNSFFEATARGVLNVVRTRPAALPVLNQQ 754

BLAST of Lag0031011 vs. TAIR 10
Match: AT3G53760.1 (GAMMA-TUBULIN COMPLEX PROTEIN 4 )

HSP 1 Score: 1065.1 bits (2753), Expect = 2.6e-311
Identity = 562/749 (75.03%), Postives = 630/749 (84.11%), Query Frame = 0

Query: 1   MLHELLLALLGYTGDLIIDEREHYNSLGLNHLTPDAPISGEPTFKLAPDISFLEPSERDL 60
           MLHELLLALLG+TGDLI+DERE   +LGL     D+P+S E TFKLAPDISF+EPSERDL
Sbjct: 1   MLHELLLALLGFTGDLIVDEREQRKTLGL-AFNSDSPLSDECTFKLAPDISFIEPSERDL 60

Query: 61  IQRIIVLGFYYRELDRFATKSRNLSWIRSGNG-SLLANSAESSKEKTE-NPSVYRRAIAN 120
           I+R+I LGFYYRELDRFA KSRNLSWIRS      L  + E SK+  E  PSVYRRAIAN
Sbjct: 61  IERLIKLGFYYRELDRFAKKSRNLSWIRSVTSVHPLERADELSKQSREKKPSVYRRAIAN 120

Query: 121 GIVEILSVYRSAVLHVEQKLLSETVPILAVVTQGLDKFFVLFPPLHQLILEIERDDIRGG 180
           GI EILSVYRSAVLH+EQKLL+ET PILA VT+GL+KFFVLFPPL+++ILEIERDDIRGG
Sbjct: 121 GIGEILSVYRSAVLHIEQKLLAETTPILATVTEGLNKFFVLFPPLYEVILEIERDDIRGG 180

Query: 181 QLLNLLHKRCHCGVPELQTCIQRLLWHGHQVLYNQLASWMVYGILQDKHGEFFIRRQDDR 240
           QLLN+L+KRCHCGVPEL+TC+QRLLW+GHQV+YNQLA+WMVYGILQD HGEFFI+RQDD 
Sbjct: 181 QLLNVLNKRCHCGVPELRTCLQRLLWNGHQVMYNQLAAWMVYGILQDPHGEFFIKRQDDG 240

Query: 241 ETEQGSSVQDMSEKLGRLSTDE-SLTDWHLGFHIYLDMLPDYIHMRVAEAILFAGKAIRV 300
           + +  SS +++SEKL R S  E SLTDWH GFHI LDMLPDYI MR+ E+ILFAGKAIRV
Sbjct: 241 DLDHRSSQEEVSEKLARTSVHETSLTDWHSGFHISLDMLPDYIPMRLGESILFAGKAIRV 300

Query: 301 LRNPSNAFWCQGTGNQSHSQIPRLPLNIKGNTRNFPLQKEPFVATKLTGEELLLQSEADK 360
           LRNPS AF  Q   ++S  Q  R    I+G   +   + E  +   LTG ELL QSEADK
Sbjct: 301 LRNPSPAF--QFQKDKSFQQTMRGSQRIRGFMHSDFPETETELDADLTGGELLPQSEADK 360

Query: 361 IEAMLLDLKESSEFHKRSFESAVDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKG 420
           IEAML DLKESSEFHKRSFE  VDS+RAIAASHLWQLVVVRADLNGHLKALKDYFLL KG
Sbjct: 361 IEAMLKDLKESSEFHKRSFECTVDSVRAIAASHLWQLVVVRADLNGHLKALKDYFLLEKG 420

Query: 421 DFFQCFLEESRHLMRLPPRQSTAEADLMIPFQLAATKTTSEEDKYFSRVSLRMPSFGVKV 480
           DFFQCFLEESR LMRLPPRQST E+DLM+PFQLAATKT +EEDKYFSRVSLRMPSFGV V
Sbjct: 421 DFFQCFLEESRQLMRLPPRQSTGESDLMVPFQLAATKTIAEEDKYFSRVSLRMPSFGVTV 480

Query: 481 KSAQGDSKEKSYTDGNLGGALSNLSSDMSLDGWDGVALEYYIDWPLQLFFTQEVLSKYCR 540
           +S+Q D         +L G  +NL+SD S+DGWD +ALEY +DWP+QLFFTQEVLSKY +
Sbjct: 481 RSSQADMVRSKV---SLTGK-ANLTSDTSVDGWDAIALEYSVDWPMQLFFTQEVLSKYLK 540

Query: 541 VFQYLLRLKRTQMELEKSWASLMHQDHADFAKNRRARFDGSISWQ-RQRFRRMWRVREHM 600
           VFQYL+RLKRTQMELEKSWAS+MHQDH + A++R+   +GS S Q RQ  R MWRVREHM
Sbjct: 541 VFQYLIRLKRTQMELEKSWASVMHQDHIESAQHRKDGLNGSTSQQRRQGIRPMWRVREHM 600

Query: 601 AFLIRNLQFYIQVDVIESQWNILQDHIQDSHDFTELVGFHQEYLSALISQSFLDIGSVSR 660
           AFLIRNLQFYIQVDVIESQW +LQ HI DS DFTELVGFHQEYLSALISQSFLDIGSVSR
Sbjct: 601 AFLIRNLQFYIQVDVIESQWKVLQTHIHDSQDFTELVGFHQEYLSALISQSFLDIGSVSR 660

Query: 661 ILDGIMKLCLQFCWSIENQDSNSDPPELEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFL 720
           ILD IMKLCLQFCW+IENQ+SN +  ELE++ EEFNKKSNSLYTILRSS+L GSQRAPFL
Sbjct: 661 ILDSIMKLCLQFCWNIENQESNPNTSELENIAEEFNKKSNSLYTILRSSKLAGSQRAPFL 720

Query: 721 RRFLMRLNFNSFFEATARGVLNVVRPRPA 746
           RRFL+RLNFNSF+EATARGVLNVVR RPA
Sbjct: 721 RRFLLRLNFNSFYEATARGVLNVVRQRPA 742

BLAST of Lag0031011 vs. TAIR 10
Match: AT5G06680.1 (spindle pole body component 98 )

HSP 1 Score: 100.9 bits (250), Expect = 4.6e-21
Identity = 118/477 (24.74%), Postives = 191/477 (40.04%), Query Frame = 0

Query: 175 IRGGQLLNLLHKRCHCGVPELQTCIQRLLWHGHQVLYNQLASWMVYGILQDKHGEFFIRR 234
           +RGG +   +H     G P +   +  LL      L+  + SW++ G L+D  GEFF+  
Sbjct: 334 LRGGAMAGAIHLHAQHGDPLVHDFMMSLLRCVCSPLFEMVRSWVLEGELEDTFGEFFVVG 393

Query: 235 QDDRETEQGSSVQDMSEKLGRLSTDESLTDWHLGFHIYLDMLPDYIHMRVAEAILFAGKA 294
           Q                K+  L        W  G+ ++  MLP +I   +A+ IL  GK+
Sbjct: 394 Q--------------PVKVDLL--------WREGYKLHPAMLPSFISPSLAQRILRTGKS 453

Query: 295 IRVLRNPSNAF-WCQGTGNQSHSQIPRLPLNIKGNTRNFPLQKEPFVATKLTGEELLLQS 354
           I  LR   +   W       + +          G T            T+  G   L   
Sbjct: 454 INFLRVCCDDHGWADAASEAAAA---------SGTT------------TRRGG---LGYG 513

Query: 355 EADKIEAMLLDLKESSEFHKRSFESAVDSIRAIAASHLWQLVVVRADLNGHLKALKDYFL 414
           E D +E ++      +E  KR     +D        HL  ++  R     H  A+K Y L
Sbjct: 514 ETDALEHLV------TEAAKR-----ID-------KHLLDVLYKRYKFKEHCLAIKRYLL 573

Query: 415 LAKGDFFQCFLEESRHLMRLPPRQSTAEADLMIPFQLAATKTTSEEDKYFSRVSLRMPSF 474
           L +GDF Q       +LM +   + +  A+ +  F+LA     +                
Sbjct: 574 LGQGDFVQ-------YLMDIVGPKLSEPANNISSFELAGFLEAA---------------- 633

Query: 475 GVKVKSAQGDSKEKSYTDGNLGGALSNLSSDMSLDGWDGVALEYYIDWPLQLFFTQEVLS 534
            ++  +AQ D  ++   D      + + S D    GWD  +LEY    PL   FT+ VLS
Sbjct: 634 -IRASNAQYD--DRDMLDRLRVKMMPHGSGDR---GWDVFSLEYEARVPLDTVFTESVLS 693

Query: 535 KYCRVFQYLLRLKRTQMELEKSWASLMHQDHADFAKNRRARFDGSISWQ-RQRFRRMWRV 594
           KY RVF +L +LKR +  L   W ++          N   +   S+  Q     RR   +
Sbjct: 694 KYLRVFNFLWKLKRVEHALIGIWKTMKPN---CITSNSFVKLQSSVKLQLLSALRRCQVL 714

Query: 595 REHMAFLIRNLQFYIQVDVIESQWNILQDHIQDSHDFTELVGFHQEYLSALISQSFL 650
              M   + N Q+YI  +V+E  W+     ++ + D  +L+  H++YL+A++ +S L
Sbjct: 754 WNEMNHFVTNFQYYIMFEVLEVSWSNFSKEMEAAKDLDDLLAAHEKYLNAIVGKSLL 714

BLAST of Lag0031011 vs. TAIR 10
Match: AT5G17410.2 (Spc97 / Spc98 family of spindle pole body (SBP) component )

HSP 1 Score: 84.3 bits (207), Expect = 4.4e-16
Identity = 81/355 (22.82%), Postives = 150/355 (42.25%), Query Frame = 0

Query: 380 VDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDFFQCFLEESRHLMRLPPRQST 439
           + +    A+  L  L+  + DL G L+++K Y LL +GDF   F++ +R  +     + +
Sbjct: 342 IKAAHEFASIELVNLIKDKYDLVGRLRSIKHYLLLDQGDFLVHFMDIAREELNKKVHEIS 401

Query: 440 AEADLMIPFQLAATKTTSEEDKYFSRVSLRMPSFGVKVKSAQGDSKEKSYTDGNLGGALS 499
            E  L     LA   T +  D     ++  +      + +  G  K+   TD N      
Sbjct: 402 VE-KLQSLLDLALRTTAAAADPRHEDLTCCVDR--ASLLTTLGMHKD---TDSN------ 461

Query: 500 NLSSDMSLDGWDGVALEYYIDWPLQLFFTQEVLSKYCRVFQYLLRLKRTQMELEKSWASL 559
           ++   MS+ G +  +L Y + WPL +  +++ LSKY  +F++L   K  + +L  +W   
Sbjct: 462 SIEDPMSITGLETFSLSYKVQWPLSIVISKKALSKYQLIFRFLFHCKHVERQLCGAWQ-- 521

Query: 560 MHQDHADFAKNRRARFDGSISWQRQRFRRMWRVREHMAFLIRNLQFYIQVDVIESQWNIL 619
           +HQ          A    S+            +   M   I +L  Y+  +V+E  W+++
Sbjct: 522 IHQGIRSMNSKGTAILRSSL------------LCRSMLKFISSLLHYLTFEVLEPNWHVM 581

Query: 620 QDHIQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRILDGIMKLCLQFC----WSIENQ 679
            D +Q +    E++  H  +L   +    L +  V + ++ +  +CLQ+     W I + 
Sbjct: 582 HDRLQSTRSVDEVIQHHDFFLDKCLRGCLLLLPDVLKKMEKLKSVCLQYAAATQWLISSS 641

Query: 680 ---DSNSDPPE-----------LEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFL 717
              +S S P +           + +   EFN +  SL  +L      GSQ  P+L
Sbjct: 642 IDINSQSHPQKTMIRDTTVTESIFNFEREFNSELQSLGPVLSK----GSQAEPYL 666

BLAST of Lag0031011 vs. TAIR 10
Match: AT5G17410.1 (Spc97 / Spc98 family of spindle pole body (SBP) component )

HSP 1 Score: 84.3 bits (207), Expect = 4.4e-16
Identity = 81/355 (22.82%), Postives = 150/355 (42.25%), Query Frame = 0

Query: 380 VDSIRAIAASHLWQLVVVRADLNGHLKALKDYFLLAKGDFFQCFLEESRHLMRLPPRQST 439
           + +    A+  L  L+  + DL G L+++K Y LL +GDF   F++ +R  +     + +
Sbjct: 341 IKAAHEFASIELVNLIKDKYDLVGRLRSIKHYLLLDQGDFLVHFMDIAREELNKKVHEIS 400

Query: 440 AEADLMIPFQLAATKTTSEEDKYFSRVSLRMPSFGVKVKSAQGDSKEKSYTDGNLGGALS 499
            E  L     LA   T +  D     ++  +      + +  G  K+   TD N      
Sbjct: 401 VE-KLQSLLDLALRTTAAAADPRHEDLTCCVDR--ASLLTTLGMHKD---TDSN------ 460

Query: 500 NLSSDMSLDGWDGVALEYYIDWPLQLFFTQEVLSKYCRVFQYLLRLKRTQMELEKSWASL 559
           ++   MS+ G +  +L Y + WPL +  +++ LSKY  +F++L   K  + +L  +W   
Sbjct: 461 SIEDPMSITGLETFSLSYKVQWPLSIVISKKALSKYQLIFRFLFHCKHVERQLCGAWQ-- 520

Query: 560 MHQDHADFAKNRRARFDGSISWQRQRFRRMWRVREHMAFLIRNLQFYIQVDVIESQWNIL 619
           +HQ          A    S+            +   M   I +L  Y+  +V+E  W+++
Sbjct: 521 IHQGIRSMNSKGTAILRSSL------------LCRSMLKFISSLLHYLTFEVLEPNWHVM 580

Query: 620 QDHIQDSHDFTELVGFHQEYLSALISQSFLDIGSVSRILDGIMKLCLQFC----WSIENQ 679
            D +Q +    E++  H  +L   +    L +  V + ++ +  +CLQ+     W I + 
Sbjct: 581 HDRLQSTRSVDEVIQHHDFFLDKCLRGCLLLLPDVLKKMEKLKSVCLQYAAATQWLISSS 640

Query: 680 ---DSNSDPPE-----------LEHLTEEFNKKSNSLYTILRSSRLVGSQRAPFL 717
              +S S P +           + +   EFN +  SL  +L      GSQ  P+L
Sbjct: 641 IDINSQSHPQKTMIRDTTVTESIFNFEREFNSELQSLGPVLSK----GSQAEPYL 665

BLAST of Lag0031011 vs. TAIR 10
Match: AT3G43610.1 (Spc97 / Spc98 family of spindle pole body (SBP) component )

HSP 1 Score: 52.8 bits (125), Expect = 1.4e-06
Identity = 54/271 (19.93%), Postives = 104/271 (38.38%), Query Frame = 0

Query: 401  LNGHLKALKDYFLLAKGDFFQCFLEESRHLMRLPPRQSTAEADLMIPFQLAATKTTSEED 460
            L  HL AL+ Y  +   D+   F+    H   L        A++    + +  +++ E D
Sbjct: 879  LQEHLLALRRYHFMELADWADVFVVSLWHHKWLVTEADKRIAEIQGFLESSIQRSSCERD 938

Query: 461  KYFSRVSLRMPSFGVKVKSAQGDSKEKSYTDGNLGGALSNLSSDMSLDGWDGVALEYYID 520
                R+ L                           G +    S + +  +D + L Y +D
Sbjct: 939  ICKDRIFLYKRQ-----------------------GTMHIPPSTIGVRSFDFLRLGYRVD 998

Query: 521  WPLQLFFTQEVLSKYCRVFQYLLRLKRTQMELEKSWASLMHQDHADFAKNRRARFDGSIS 580
            WP+ +  T + L+ Y  VF +L+++K     L   W SL    H    K  +      + 
Sbjct: 999  WPISIILTCDALTAYADVFSFLVQVKLAAYVLTDVWCSLKDVRHMMHEKKEKI-----LK 1058

Query: 581  WQRQRFRRMWRVREHMAFLIRNLQFYIQVDVIESQWNILQDHIQDS-HDFTELVGFHQEY 640
             + +    + ++R  +   +  LQ Y+  ++    W+     +++   D  +L   H  Y
Sbjct: 1059 QELRWLNILMKLRHQVNHFVTALQQYVHSELSHVSWSKFLHSLKNKVKDMMDLESVHMAY 1118

Query: 641  LSALISQSFL--DIGSVSRILDGIMKLCLQF 669
            LS  +   FL  +   +S I++ I++  L F
Sbjct: 1119 LSEALRICFLSDETQIISNIIENILQCALDF 1121

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038879485.10.0e+0094.96gamma-tubulin complex component 4 homolog [Benincasa hispida][more]
XP_011660150.10.0e+0094.96gamma-tubulin complex component 4 homolog isoform X1 [Cucumis sativus] >XP_01166... [more]
XP_022934806.10.0e+0094.69gamma-tubulin complex component 4 homolog [Cucurbita moschata] >XP_022934807.1 g... [more]
XP_023528229.10.0e+0094.43gamma-tubulin complex component 4 homolog [Cucurbita pepo subsp. pepo][more]
KAA0035230.10.0e+0094.83gamma-tubulin complex component 4-like protein [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
Q9SC887.6e-31373.88Gamma-tubulin complex component 4 homolog OS=Medicago truncatula OX=3880 GN=85P ... [more]
Q9M3503.7e-31075.03Gamma-tubulin complex component 4 OS=Arabidopsis thaliana OX=3702 GN=GCP4 PE=2 S... [more]
Q9UGJ15.0e-8129.33Gamma-tubulin complex component 4 OS=Homo sapiens OX=9606 GN=TUBGCP4 PE=1 SV=1[more]
Q9D4F83.3e-8029.73Gamma-tubulin complex component 4 OS=Mus musculus OX=10090 GN=Tubgcp4 PE=1 SV=2[more]
Q9VKU77.3e-4024.47Gamma-tubulin complex component 4 homolog OS=Drosophila melanogaster OX=7227 GN=... [more]
Match NameE-valueIdentityDescription
A0A6J1F2V20.0e+0094.69Gamma-tubulin complex component OS=Cucurbita moschata OX=3662 GN=LOC111441869 PE... [more]
A0A5A7SW230.0e+0094.83Gamma-tubulin complex component OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_... [more]
A0A6J1DP060.0e+0094.43Gamma-tubulin complex component OS=Momordica charantia OX=3673 GN=LOC111022444 P... [more]
A0A5A7VFR50.0e+0094.57Gamma-tubulin complex component OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_... [more]
A0A6J1J9A70.0e+0093.63Gamma-tubulin complex component OS=Cucurbita maxima OX=3661 GN=LOC111482469 PE=3... [more]
Match NameE-valueIdentityDescription
AT3G53760.12.6e-31175.03GAMMA-TUBULIN COMPLEX PROTEIN 4 [more]
AT5G06680.14.6e-2124.74spindle pole body component 98 [more]
AT5G17410.24.4e-1622.82Spc97 / Spc98 family of spindle pole body (SBP) component [more]
AT5G17410.14.4e-1622.82Spc97 / Spc98 family of spindle pole body (SBP) component [more]
AT3G43610.11.4e-0619.93Spc97 / Spc98 family of spindle pole body (SBP) component [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR041470Gamma tubulin complex component protein, N-terminalPFAMPF17681GCP_N_terminalcoord: 2..396
e-value: 3.1E-49
score: 168.3
IPR040457Gamma tubulin complex component, C-terminalPFAMPF04130GCP_C_terminalcoord: 401..729
e-value: 7.6E-57
score: 193.1
IPR042241Gamma-tubulin complex, C-terminal domain superfamilyGENE3D1.20.120.1900coord: 401..728
e-value: 2.3E-85
score: 288.4
IPR007259Gamma-tubulin complex component proteinPANTHERPTHR19302GAMMA TUBULIN COMPLEX PROTEINcoord: 1..743
NoneNo IPR availablePANTHERPTHR19302:SF27GAMMA-TUBULIN COMPLEX COMPONENT 4coord: 1..743

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0031011.1Lag0031011.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0031122 cytoplasmic microtubule organization
biological_process GO:0051321 meiotic cell cycle
biological_process GO:0007020 microtubule nucleation
biological_process GO:0000278 mitotic cell cycle
biological_process GO:0051225 spindle assembly
biological_process GO:0000226 microtubule cytoskeleton organization
cellular_component GO:0005737 cytoplasm
cellular_component GO:0000923 equatorial microtubule organizing center
cellular_component GO:0000930 gamma-tubulin complex
cellular_component GO:0005874 microtubule
cellular_component GO:0000922 spindle pole
cellular_component GO:0005815 microtubule organizing center
molecular_function GO:0043015 gamma-tubulin binding
molecular_function GO:0051011 microtubule minus-end binding