Lag0030990 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0030990 vs. NCBI nr
Match: XP_038904034.1 (translocase of chloroplast 159, chloroplastic [Benincasa hispida])

HSP 1 Score: 1595.9 bits (4131), Expect = 0.0e+00
Identity = 887/1152 (77.00%), Postives = 932/1152 (80.90%), Query Frame = 0

Query: 1    MEKGSLVAEKQADGEVDLNDKVAAEDGEQLTKLETSSPVEDKAVLGDDENSKNLEPADGE 60
            MEKGS VAEKQ DGEV+LN+KVAAEDG+QL +LET SP+EDKAVLG     K LEPADG 
Sbjct: 329  MEKGSFVAEKQVDGEVELNEKVAAEDGKQLKELETGSPIEDKAVLG----VKVLEPADGG 388

Query: 61   QEAEMDKGSPVAEKQEEEEIKLNDIVDAEDGERLTKLETGSSFDNKADQDDQANSKVSDL 120
            QEAE+D+GSPVA+ Q   E+ LND VDAEDG+ LTKLE  SS DNKAD DDQ N +V++L
Sbjct: 389  QEAEIDEGSPVADIQAVGEVSLNDKVDAEDGKLLTKLEPVSSVDNKADHDDQVNPEVAEL 448

Query: 121  ADESTGAVLDDKILHDSSPVSGTDAVGNPEEIKDVENREIADLVHGAAKLDNGFDNDGHE 180
            ADE TG+VLDDK LH+SS VS TDAVGNP+EIKDVENRE ADLVHGAAKLDNGFDN GHE
Sbjct: 449  ADEFTGSVLDDKTLHESSQVSATDAVGNPDEIKDVENRETADLVHGAAKLDNGFDNVGHE 508

Query: 181  ADESVEHNSVVSSTEIDNKVSEVSIAVATEEAAAHVDRAIAAADIAKNENLEVTDVEDQQ 240
             DE V+HNSVVS++EI+N V +VS AVAT+E A H DRAIAA+DIAK+ENL   DVEDQQ
Sbjct: 509  VDEPVDHNSVVSNSEINNNVPDVSTAVATQEVAPHGDRAIAASDIAKSENLADMDVEDQQ 568

Query: 241  PAELGASTVNEEREIVSLVDSPAEAGNEKDSKDNFEAREVVDGEVESEPSQEATSLVKEN 300
            P   GASTVNEERE V+LVDSP EAGNEKDSK + + RE V G+VESEPSQE  SLVKE+
Sbjct: 569  PDGDGASTVNEERETVNLVDSPTEAGNEKDSKFDSKIREDVPGDVESEPSQEDRSLVKES 628

Query: 301  IPDNASLEDSRISDAPKIHEPVLNEVDGGKHPLDEEGDIEGSITDGETEGEIFGSSEAAR 360
            IPDNAS+EDS IS APK+ EPVLNEVDG K PLDEEG IEGS TDGETEGEIFGSSEAAR
Sbjct: 629  IPDNASVEDSGISVAPKLLEPVLNEVDGEKQPLDEEGGIEGSGTDGETEGEIFGSSEAAR 688

Query: 361  EFLQELERASGAGSHSGADSSIDHSQRIDGQIVTDSDEADTDDEGDGKQLFDSAALAALL 420
            EFLQELERASGAGSHSGADSSIDHSQRIDGQI+TDSDEADTDDEGDGK+LFDSAALAALL
Sbjct: 689  EFLQELERASGAGSHSGADSSIDHSQRIDGQIITDSDEADTDDEGDGKELFDSAALAALL 748

Query: 421  KAARDAGSDGGPITVTSQDGSRLFSIERPAGLGSSLTSGKNASRPSRPLSFAPSNPR--- 480
            KAARDAGSDGGPITVTSQDGSRLFSIERPAGLGSSL SGKNASRPSRPL+FAPSNPR   
Sbjct: 749  KAARDAGSDGGPITVTSQDGSRLFSIERPAGLGSSLISGKNASRPSRPLTFAPSNPRMGD 808

Query: 481  -----------------------------RLGVSPDDSLVAQVLYRFGLVAGRSS----- 540
                                         RLGVSPDDSLVAQVLYRFGLVAGRS+     
Sbjct: 809  DSENKLSEEEKTKLQKLQQIRVNFLRLVQRLGVSPDDSLVAQVLYRFGLVAGRSTGQLFS 868

Query: 541  ----------------------------GKSGVGKSATINSIFGEDKTPINAFGPGTTTV 600
                                        GKSGVGKSATINSIFGEDKTPINAFGPGTTTV
Sbjct: 869  FDNAKNTAIQLEAEGKEDLDFSLNILVLGKSGVGKSATINSIFGEDKTPINAFGPGTTTV 928

Query: 601  KEIVGTVEGVKIRVFDTPGLRSSSSERGINHKILFSIKNVMKKFPPDIVLYVDRLDNQTR 660
            KEIVGTVEGVKIRVFD+PGLRSSSSER INH+IL SIKNVMKKFPPDIVLYVDRLDNQTR
Sbjct: 929  KEIVGTVEGVKIRVFDSPGLRSSSSERSINHRILSSIKNVMKKFPPDIVLYVDRLDNQTR 988

Query: 661  DLNDLLLLRSVSSSLGSSIWKNAIITLTHAASAPPDGPSGSPLGYEVFVAQRSHVLQQTV 720
            DLNDLLLLRSVSSSLGSSIWKNAIITLTHAASAPPDGPSGSPLGYEVFVAQRSHVLQQTV
Sbjct: 989  DLNDLLLLRSVSSSLGSSIWKNAIITLTHAASAPPDGPSGSPLGYEVFVAQRSHVLQQTV 1048

Query: 721  AQAVGDLRMLNPTLMNPVSLVENHPSCRKNRDGQ-------------------------- 780
            AQAVGDLR+LNPTLMNPVSLVENHPSCRKNRDGQ                          
Sbjct: 1049 AQAVGDLRILNPTLMNPVSLVENHPSCRKNRDGQKVLPNGQSWRPQLLLLCFSIKILAEV 1108

Query: 781  ------------RKLFGLRARSPPLPYLLSGLLQSRTHPKLSSDQTGENGDSDIDLADLS 840
                        RK+FGLR RSPPLPYLLSGLLQSRTHPKL+SDQ G+NGDSDIDLADLS
Sbjct: 1109 GNLSKAPETFDHRKIFGLRGRSPPLPYLLSGLLQSRTHPKLASDQNGDNGDSDIDLADLS 1168

Query: 841  DSDQEEEEDEYDQLPPFKPLRKSQIAKLSKEQRKAYFEEYDYRVKLLQKKQWKEELKRMR 900
            DSDQEEEEDEYDQLPPFKPLRKSQI+KLSKEQRKAYFEEYDYRVKLLQKKQWKEELKRMR
Sbjct: 1169 DSDQEEEEDEYDQLPPFKPLRKSQISKLSKEQRKAYFEEYDYRVKLLQKKQWKEELKRMR 1228

Query: 901  DIKKKGQPTVDDYGYMGEDDQENGTPAAVQVPLPDMALPPSFDGDNPAYRFRFLEPTSQF 960
            DIKKKGQP VDDYGYMGEDDQENG+PAAVQVPLPDMALPPSFDGDNPAYRFRFLEPTSQF
Sbjct: 1229 DIKKKGQPNVDDYGYMGEDDQENGSPAAVQVPLPDMALPPSFDGDNPAYRFRFLEPTSQF 1288

Query: 961  LARPVLDTHGWDHDCGYDGVNLEHSMAIVSRFPAAVAVQITKDKKEFSIHLDSSVSAKHG 1006
            LARPVLDTHGWDHDCGYDGVNLE SMAIV+RFPAAV VQITKDKKEF+IHLDSSVSAKHG
Sbjct: 1289 LARPVLDTHGWDHDCGYDGVNLEQSMAIVNRFPAAVTVQITKDKKEFNIHLDSSVSAKHG 1348

BLAST of Lag0030990 vs. NCBI nr
Match: XP_022143380.1 (translocase of chloroplast 159, chloroplastic [Momordica charantia])

HSP 1 Score: 1530.8 bits (3962), Expect = 0.0e+00
Identity = 857/1147 (74.72%), Postives = 908/1147 (79.16%), Query Frame = 0

Query: 9    EKQADGEVDLNDKVAAEDGEQLTKLETSSPVEDKAVLGDDENSKNLEPADGEQEAEMDKG 68
            E+QADGE++LN+KV+AEDGEQL +LET SPVEDKAVL D+EN K LEPADG QE EM+KG
Sbjct: 317  ERQADGEIELNEKVSAEDGEQLKELETGSPVEDKAVLDDNENFKVLEPADGGQEVEMEKG 376

Query: 69   SPVAEKQEEEEIKLNDIVDAEDGERLTKLETGSSFDNKADQDDQANSKVSDLADESTGAV 128
            SPVAEK+ +   K ND ++AEDGE+L KLE GSS  NKAD DDQANSKVS+LADE +   
Sbjct: 377  SPVAEKEADGGTKFNDKMNAEDGEQLEKLEIGSSVVNKADLDDQANSKVSELADEFSSGC 436

Query: 129  LDDKILHDSSPVSGTDAVGNPEEIKDVENREIADLVHGAAKLDNGFDNDGHEADESVEHN 188
            LDDK LH+SS VSGTDAVGN EEIKDV NR   DLV    KLDNGFDN GHEADE V+HN
Sbjct: 437  LDDKTLHESSLVSGTDAVGNLEEIKDVGNRVSVDLVREGTKLDNGFDNVGHEADEFVDHN 496

Query: 189  SVVSSTEIDNKVSEVSIAVATEEAAAHVDRAIAAADIAKNENLEVTDVEDQQPAELGAST 248
            SVVS++EIDNK  E+SI VATEEA  H DR  AA DIA +E L   +VEDQQP +     
Sbjct: 497  SVVSNSEIDNKEPEISIPVATEEAVLHGDRGFAATDIANHEKLAAKNVEDQQPDD----- 556

Query: 249  VNEEREIVSLVDSPAEAGNEKDSKDNFEAREVVDGEVESEPSQEATSLVKENIPDNASLE 308
                RE VSL+DSP EAGNEKDSKD+ + RE+V GEVESE SQ ATSLVKE IPD+A++E
Sbjct: 557  --GLREAVSLLDSPHEAGNEKDSKDDSKIREIVAGEVESESSQGATSLVKETIPDSATVE 616

Query: 309  DSRISDAPKIHEPVLNEVDGGKHPLDEEGDIEGSITDGETEGEIFGSSEAAREFLQELER 368
            DS+IS  PKI EPVLNEVDG K+  DEEGD EGS+TDGETEGEIFGSSEAAR+FLQELER
Sbjct: 617  DSKISATPKIIEPVLNEVDGEKYHSDEEGDGEGSVTDGETEGEIFGSSEAARQFLQELER 676

Query: 369  ASGAGSHSGADSSIDHSQRIDGQIVTDSDEADTDDEGDGKQLFDSAALAALLKAARDAGS 428
            ASGAGSHSGA+SSIDHS RIDGQIVTDSDEADT+DEG+GK++FDSAALAALLKAARDAGS
Sbjct: 677  ASGAGSHSGAESSIDHSHRIDGQIVTDSDEADTEDEGEGKEMFDSAALAALLKAARDAGS 736

Query: 429  DGGPITVTSQDGSRLFSIER---PAGLGSSLTSGKNASRPSRPLSFAPSNPR-------- 488
            DGGPITVTSQDGSRLFSIER   PAGLGSSLTSGKNASRPSRPLSFA SNPR        
Sbjct: 737  DGGPITVTSQDGSRLFSIERPAGPAGLGSSLTSGKNASRPSRPLSFASSNPRVGDDSENR 796

Query: 489  ------------------------RLGVSPDDSLVAQVLYRFGLVAGRSS---------- 548
                                    RLGVSPDDSLVAQVLYRFGLVAGR++          
Sbjct: 797  LSEEEKNKLQKLQQIRVNFLRLVQRLGVSPDDSLVAQVLYRFGLVAGRATGQLFSFDNAK 856

Query: 549  -----------------------GKSGVGKSATINSIFGEDKTPINAFGPGTTTVKEIVG 608
                                   GK+GVGKSATINSIFGEDKTPINAFGPGTTTVKEIVG
Sbjct: 857  TTAIQLEAEGKEDLDFSLNILVIGKTGVGKSATINSIFGEDKTPINAFGPGTTTVKEIVG 916

Query: 609  TVEGVKIRVFDTPGLRSSSSERGINHKILFSIKNVMKKFPPDIVLYVDRLDNQTRDLNDL 668
            T+EGVKIRVFD+PGLRSSSSER INHKIL SIKNVMKK PPDIVLYVDRLDNQTRDLNDL
Sbjct: 917  TIEGVKIRVFDSPGLRSSSSERSINHKILSSIKNVMKKSPPDIVLYVDRLDNQTRDLNDL 976

Query: 669  LLLRSVSSSLGSSIWKNAIITLTHAASAPPDGPSGSPLGYEVFVAQRSHVLQQTVAQAVG 728
            LLLRS+SSSLGSSIWKNAIITLTH ASAPPDGPSG PLGYEVFVAQRSHVLQQTVAQAVG
Sbjct: 977  LLLRSISSSLGSSIWKNAIITLTHGASAPPDGPSGVPLGYEVFVAQRSHVLQQTVAQAVG 1036

Query: 729  DLRMLNPTLMNPVSLVENHPSCRKNRDGQ------------------------------- 788
            DLR+LNPTLMNPVSLVENHPSCRKNRDGQ                               
Sbjct: 1037 DLRILNPTLMNPVSLVENHPSCRKNRDGQKVLPNGQSWRPQLLLLCFSIKILAEVGSLSK 1096

Query: 789  -------RKLFGLRARSPPLPYLLSGLLQSRTHPKLSSDQTGENGDSDIDLADLSDSDQE 848
                   RKLFGLRARSPPLPYLLSGLLQSR HPKLSSDQ G+NGDSDIDLADLSDSDQE
Sbjct: 1097 APETFDHRKLFGLRARSPPLPYLLSGLLQSRAHPKLSSDQAGDNGDSDIDLADLSDSDQE 1156

Query: 849  EEEDEYDQLPPFKPLRKSQIAKLSKEQRKAYFEEYDYRVKLLQKKQWKEELKRMRDIKKK 908
            EEEDEYDQLPPFKPLRKSQIAKLSKEQR+AYFEEYDYRVKLLQKKQWKEELKRMRDIKKK
Sbjct: 1157 EEEDEYDQLPPFKPLRKSQIAKLSKEQRRAYFEEYDYRVKLLQKKQWKEELKRMRDIKKK 1216

Query: 909  GQPTVDDYGYMGEDDQENGTPAAVQVPLPDMALPPSFDGDNPAYRFRFLEPTSQFLARPV 968
            GQ   DDYGYMGEDDQENGTPAAVQVPLPDMALPPSFDGDNPAY+FRFLEPTSQFLARPV
Sbjct: 1217 GQLPADDYGYMGEDDQENGTPAAVQVPLPDMALPPSFDGDNPAYQFRFLEPTSQFLARPV 1276

Query: 969  LDTHGWDHDCGYDGVNLEHSMAIVSRFPAAVAVQITKDKKEFSIHLDSSVSAKHGENGST 1006
            LDTHGWDHDCGYDGVNLEHSMAIVSRFPAAVAVQITKDKKEF+IHLDSSVSAKHGENGST
Sbjct: 1277 LDTHGWDHDCGYDGVNLEHSMAIVSRFPAAVAVQITKDKKEFNIHLDSSVSAKHGENGST 1336

BLAST of Lag0030990 vs. NCBI nr
Match: XP_004152365.2 (translocase of chloroplast 159, chloroplastic [Cucumis sativus])

HSP 1 Score: 1515.4 bits (3922), Expect = 0.0e+00
Identity = 846/1152 (73.44%), Postives = 910/1152 (78.99%), Query Frame = 0

Query: 2    EKGSLVAEKQADGEVDLNDKV-AAEDGEQLTKLETSSPVEDKAVLGDDENSKNLEPADGE 61
            ++ +LVAEKQ D EV LND V AA+DGEQL  LET SPV++K VL DDENS  LEPADG 
Sbjct: 337  QEETLVAEKQVDVEVKLNDTVAAADDGEQLKNLETDSPVDNKIVLADDENSGVLEPADGG 396

Query: 62   QEAEMDKGSPVAEKQEEEEIKLNDIVDAEDGERLTKLETGSSFDNKADQDDQANSKVSDL 121
            QEAEM KGSPVAE Q + EI+L   VDAEDGE LTKLE  S  DNK              
Sbjct: 397  QEAEMAKGSPVAEMQADGEIRLKGKVDAEDGELLTKLEPVSFADNK-------------- 456

Query: 122  ADESTGAVLDDKILHDSSPVSGTDAVGNPEEIKDVENREIADLVHGAAKLDNGFDNDGHE 181
             DE T + LDDK LH+SS VS TD +GNPEEIKD+EN+E A+L HGA KLDNGFD+ GHE
Sbjct: 457  TDEFTASALDDKTLHESSQVSATDVLGNPEEIKDLENKETANLAHGATKLDNGFDSVGHE 516

Query: 182  ADESVEHNSVVSSTEIDNKVSEVSIAVATEEAAAHVDRAIAAADIAKNENLEVTDVEDQQ 241
             ++ V+ +SVV ++E+DN +   +IAV TEE   H +RAIAA+DIAK+ENL VTDVEDQQ
Sbjct: 517  VNQPVDSDSVVLNSEVDNSMPGANIAVGTEETEPHGNRAIAASDIAKSENLAVTDVEDQQ 576

Query: 242  PAELGASTVNEEREIVSLVDSPAEAGNEKDSKDNFEAREVVDGEVESEPSQEATSLVKEN 301
               +GASTVNEERE V+L DSP++AGNEKDSKD+ + RE V G+VESEPSQE   L+KE+
Sbjct: 577  LDGVGASTVNEERETVNLADSPSKAGNEKDSKDDSKIREDVPGDVESEPSQEDRGLIKES 636

Query: 302  IPDNASLEDSRISDAPKIHEPVLNEVDGGKHPLDEEGDIEGSITDGETEGEIFGSSEAAR 361
            IPDNAS++DS ISDAPK+ EPVL+EVDG KHPLDEEGDIEGS TDGETE EIFGSSEAAR
Sbjct: 637  IPDNASVKDSGISDAPKLLEPVLSEVDGEKHPLDEEGDIEGSGTDGETEAEIFGSSEAAR 696

Query: 362  EFLQELERASGAGSHSGADSSIDHSQRIDGQIVTDSDEADTDDEGDGKQLFDSAALAALL 421
            EFLQELERASGAGSHSGA+SSIDHSQRIDGQIVTDSDEADT+DEGDGK+LFDSAALAALL
Sbjct: 697  EFLQELERASGAGSHSGAESSIDHSQRIDGQIVTDSDEADTEDEGDGKELFDSAALAALL 756

Query: 422  KAARDAGSDGGPITVTSQDGSRLFSIERPAGLGSSLTSGKNASRPSRPLSFAPSNPR--- 481
            KAARDAGSDGGPITVT+QDGSRLFSIERPAGLGSSL SGKNASRPSRPL+FA SNPR   
Sbjct: 757  KAARDAGSDGGPITVTTQDGSRLFSIERPAGLGSSLISGKNASRPSRPLTFASSNPRVGD 816

Query: 482  -----------------------------RLGVSPDDSLVAQVLYRFGLVAGRSS----- 541
                                         RLGVSPDDSLVAQVLYRFGLVAGRS+     
Sbjct: 817  DAENKLSEEEKTKLQKLQKIRVNFLRLVQRLGVSPDDSLVAQVLYRFGLVAGRSTGQLFS 876

Query: 542  ----------------------------GKSGVGKSATINSIFGEDKTPINAFGPGTTTV 601
                                        GKSGVGKSATINSIFGE+KTPINAFGPGTTTV
Sbjct: 877  FDNAKNTAIQLEAEGKEDLDFSLNILVLGKSGVGKSATINSIFGENKTPINAFGPGTTTV 936

Query: 602  KEIVGTVEGVKIRVFDTPGLRSSSSERGINHKILFSIKNVMKKFPPDIVLYVDRLDNQTR 661
            KEI+GTVEGVKIRVFD+PGLRSSSSER IN++IL SIKNVMKKFPPDIVLYVDRLDNQTR
Sbjct: 937  KEIIGTVEGVKIRVFDSPGLRSSSSERRINNRILSSIKNVMKKFPPDIVLYVDRLDNQTR 996

Query: 662  DLNDLLLLRSVSSSLGSSIWKNAIITLTHAASAPPDGPSGSPLGYEVFVAQRSHVLQQTV 721
            DLNDLLLLRSVSSSLGSSIWKNAIITLTHAASAPPDGPSGSPLGYEVFVAQRSHVLQQTV
Sbjct: 997  DLNDLLLLRSVSSSLGSSIWKNAIITLTHAASAPPDGPSGSPLGYEVFVAQRSHVLQQTV 1056

Query: 722  AQAVGDLRMLNPTLMNPVSLVENHPSCRKNRDGQ-------------------------- 781
            AQAVGDLR+LNPTLMNPVSLVENHPSCRKNRDGQ                          
Sbjct: 1057 AQAVGDLRILNPTLMNPVSLVENHPSCRKNRDGQKVLPNGQTWRPQLLLLCFSIKILAEV 1116

Query: 782  ------------RKLFGLRARSPPLPYLLSGLLQSRTHPKLSSDQTGENGDSDIDLADLS 841
                        RK+FGLR RSPPLPYLLSGLLQSRTHPKL+SDQ+G+NGDSDIDLAD+S
Sbjct: 1117 GNLSKAPETFDHRKIFGLRGRSPPLPYLLSGLLQSRTHPKLASDQSGDNGDSDIDLADMS 1176

Query: 842  DSDQEEEEDEYDQLPPFKPLRKSQIAKLSKEQRKAYFEEYDYRVKLLQKKQWKEELKRMR 901
            DSDQEEEEDEYDQLPPFKPLRKSQI+KLSKEQRKAYFEEYDYRVKLLQKKQWKEELKRMR
Sbjct: 1177 DSDQEEEEDEYDQLPPFKPLRKSQISKLSKEQRKAYFEEYDYRVKLLQKKQWKEELKRMR 1236

Query: 902  DIKKKGQPTVDDYGYMGEDDQENGTPAAVQVPLPDMALPPSFDGDNPAYRFRFLEPTSQF 961
            DIKKKGQPTV+DYGYMGEDDQEN +PAAVQVPLPDMALPPSFDGDNPAYRFRFLEPTSQF
Sbjct: 1237 DIKKKGQPTVNDYGYMGEDDQENSSPAAVQVPLPDMALPPSFDGDNPAYRFRFLEPTSQF 1296

Query: 962  LARPVLDTHGWDHDCGYDGVNLEHSMAIVSRFPAAVAVQITKDKKEFSIHLDSSVSAKHG 1006
            LARPVLDTHGWDHDCGYDGVNLEHSMAIV+RFPAAVAVQITKDKKEF+IHLDSSVSAKHG
Sbjct: 1297 LARPVLDTHGWDHDCGYDGVNLEHSMAIVNRFPAAVAVQITKDKKEFNIHLDSSVSAKHG 1356

BLAST of Lag0030990 vs. NCBI nr
Match: KAE8649040.1 (hypothetical protein Csa_014625 [Cucumis sativus])

HSP 1 Score: 1515.4 bits (3922), Expect = 0.0e+00
Identity = 846/1152 (73.44%), Postives = 910/1152 (78.99%), Query Frame = 0

Query: 2    EKGSLVAEKQADGEVDLNDKV-AAEDGEQLTKLETSSPVEDKAVLGDDENSKNLEPADGE 61
            ++ +LVAEKQ D EV LND V AA+DGEQL  LET SPV++K VL DDENS  LEPADG 
Sbjct: 291  QEETLVAEKQVDVEVKLNDTVAAADDGEQLKNLETDSPVDNKIVLADDENSGVLEPADGG 350

Query: 62   QEAEMDKGSPVAEKQEEEEIKLNDIVDAEDGERLTKLETGSSFDNKADQDDQANSKVSDL 121
            QEAEM KGSPVAE Q + EI+L   VDAEDGE LTKLE  S  DNK              
Sbjct: 351  QEAEMAKGSPVAEMQADGEIRLKGKVDAEDGELLTKLEPVSFADNK-------------- 410

Query: 122  ADESTGAVLDDKILHDSSPVSGTDAVGNPEEIKDVENREIADLVHGAAKLDNGFDNDGHE 181
             DE T + LDDK LH+SS VS TD +GNPEEIKD+EN+E A+L HGA KLDNGFD+ GHE
Sbjct: 411  TDEFTASALDDKTLHESSQVSATDVLGNPEEIKDLENKETANLAHGATKLDNGFDSVGHE 470

Query: 182  ADESVEHNSVVSSTEIDNKVSEVSIAVATEEAAAHVDRAIAAADIAKNENLEVTDVEDQQ 241
             ++ V+ +SVV ++E+DN +   +IAV TEE   H +RAIAA+DIAK+ENL VTDVEDQQ
Sbjct: 471  VNQPVDSDSVVLNSEVDNSMPGANIAVGTEETEPHGNRAIAASDIAKSENLAVTDVEDQQ 530

Query: 242  PAELGASTVNEEREIVSLVDSPAEAGNEKDSKDNFEAREVVDGEVESEPSQEATSLVKEN 301
               +GASTVNEERE V+L DSP++AGNEKDSKD+ + RE V G+VESEPSQE   L+KE+
Sbjct: 531  LDGVGASTVNEERETVNLADSPSKAGNEKDSKDDSKIREDVPGDVESEPSQEDRGLIKES 590

Query: 302  IPDNASLEDSRISDAPKIHEPVLNEVDGGKHPLDEEGDIEGSITDGETEGEIFGSSEAAR 361
            IPDNAS++DS ISDAPK+ EPVL+EVDG KHPLDEEGDIEGS TDGETE EIFGSSEAAR
Sbjct: 591  IPDNASVKDSGISDAPKLLEPVLSEVDGEKHPLDEEGDIEGSGTDGETEAEIFGSSEAAR 650

Query: 362  EFLQELERASGAGSHSGADSSIDHSQRIDGQIVTDSDEADTDDEGDGKQLFDSAALAALL 421
            EFLQELERASGAGSHSGA+SSIDHSQRIDGQIVTDSDEADT+DEGDGK+LFDSAALAALL
Sbjct: 651  EFLQELERASGAGSHSGAESSIDHSQRIDGQIVTDSDEADTEDEGDGKELFDSAALAALL 710

Query: 422  KAARDAGSDGGPITVTSQDGSRLFSIERPAGLGSSLTSGKNASRPSRPLSFAPSNPR--- 481
            KAARDAGSDGGPITVT+QDGSRLFSIERPAGLGSSL SGKNASRPSRPL+FA SNPR   
Sbjct: 711  KAARDAGSDGGPITVTTQDGSRLFSIERPAGLGSSLISGKNASRPSRPLTFASSNPRVGD 770

Query: 482  -----------------------------RLGVSPDDSLVAQVLYRFGLVAGRSS----- 541
                                         RLGVSPDDSLVAQVLYRFGLVAGRS+     
Sbjct: 771  DAENKLSEEEKTKLQKLQKIRVNFLRLVQRLGVSPDDSLVAQVLYRFGLVAGRSTGQLFS 830

Query: 542  ----------------------------GKSGVGKSATINSIFGEDKTPINAFGPGTTTV 601
                                        GKSGVGKSATINSIFGE+KTPINAFGPGTTTV
Sbjct: 831  FDNAKNTAIQLEAEGKEDLDFSLNILVLGKSGVGKSATINSIFGENKTPINAFGPGTTTV 890

Query: 602  KEIVGTVEGVKIRVFDTPGLRSSSSERGINHKILFSIKNVMKKFPPDIVLYVDRLDNQTR 661
            KEI+GTVEGVKIRVFD+PGLRSSSSER IN++IL SIKNVMKKFPPDIVLYVDRLDNQTR
Sbjct: 891  KEIIGTVEGVKIRVFDSPGLRSSSSERRINNRILSSIKNVMKKFPPDIVLYVDRLDNQTR 950

Query: 662  DLNDLLLLRSVSSSLGSSIWKNAIITLTHAASAPPDGPSGSPLGYEVFVAQRSHVLQQTV 721
            DLNDLLLLRSVSSSLGSSIWKNAIITLTHAASAPPDGPSGSPLGYEVFVAQRSHVLQQTV
Sbjct: 951  DLNDLLLLRSVSSSLGSSIWKNAIITLTHAASAPPDGPSGSPLGYEVFVAQRSHVLQQTV 1010

Query: 722  AQAVGDLRMLNPTLMNPVSLVENHPSCRKNRDGQ-------------------------- 781
            AQAVGDLR+LNPTLMNPVSLVENHPSCRKNRDGQ                          
Sbjct: 1011 AQAVGDLRILNPTLMNPVSLVENHPSCRKNRDGQKVLPNGQTWRPQLLLLCFSIKILAEV 1070

Query: 782  ------------RKLFGLRARSPPLPYLLSGLLQSRTHPKLSSDQTGENGDSDIDLADLS 841
                        RK+FGLR RSPPLPYLLSGLLQSRTHPKL+SDQ+G+NGDSDIDLAD+S
Sbjct: 1071 GNLSKAPETFDHRKIFGLRGRSPPLPYLLSGLLQSRTHPKLASDQSGDNGDSDIDLADMS 1130

Query: 842  DSDQEEEEDEYDQLPPFKPLRKSQIAKLSKEQRKAYFEEYDYRVKLLQKKQWKEELKRMR 901
            DSDQEEEEDEYDQLPPFKPLRKSQI+KLSKEQRKAYFEEYDYRVKLLQKKQWKEELKRMR
Sbjct: 1131 DSDQEEEEDEYDQLPPFKPLRKSQISKLSKEQRKAYFEEYDYRVKLLQKKQWKEELKRMR 1190

Query: 902  DIKKKGQPTVDDYGYMGEDDQENGTPAAVQVPLPDMALPPSFDGDNPAYRFRFLEPTSQF 961
            DIKKKGQPTV+DYGYMGEDDQEN +PAAVQVPLPDMALPPSFDGDNPAYRFRFLEPTSQF
Sbjct: 1191 DIKKKGQPTVNDYGYMGEDDQENSSPAAVQVPLPDMALPPSFDGDNPAYRFRFLEPTSQF 1250

Query: 962  LARPVLDTHGWDHDCGYDGVNLEHSMAIVSRFPAAVAVQITKDKKEFSIHLDSSVSAKHG 1006
            LARPVLDTHGWDHDCGYDGVNLEHSMAIV+RFPAAVAVQITKDKKEF+IHLDSSVSAKHG
Sbjct: 1251 LARPVLDTHGWDHDCGYDGVNLEHSMAIVNRFPAAVAVQITKDKKEFNIHLDSSVSAKHG 1310

BLAST of Lag0030990 vs. NCBI nr
Match: XP_022935299.1 (translocase of chloroplast 159, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 1506.1 bits (3898), Expect = 0.0e+00
Identity = 849/1160 (73.19%), Postives = 903/1160 (77.84%), Query Frame = 0

Query: 1    MEKGSLVAEKQADGEVDLNDKVAAEDGEQLTKLETSSPVEDKAVLGDD--------ENSK 60
            M+KGS VAE QAD ++ LN+   AEDGE+L KLE+ S V+ KA   D         ENSK
Sbjct: 530  MDKGSSVAEMQADRKIILNEMEDAEDGERLPKLESGSSVDSKADQDDQANSKVAELENSK 589

Query: 61   NLEPADGEQEAEMDKGSPVAEKQEEEEIKLNDIVDAEDGERLTKLETGSSFDNKADQDDQ 120
             LE ADG +EAE+DKGSPVAE Q + EI LND  DAEDGE+  KLE GSS D+KADQDDQ
Sbjct: 590  FLELADGGKEAEVDKGSPVAEIQADGEIILNDKEDAEDGEQFPKLEPGSSVDSKADQDDQ 649

Query: 121  ANSKVSDLADESTGAVLDDKILHDSSPVSGTDAVGNPEEIKDVENREIADLVHGAAKLDN 180
            ANSKV++LADE T +VLD+KILH+SS VS T AVGNP EIKDV NRE ADLVHGAAKLDN
Sbjct: 650  ANSKVAELADEFTDSVLDNKILHESSLVSVTAAVGNPVEIKDVGNRETADLVHGAAKLDN 709

Query: 181  GFDNDGHEADESVEHNSVVSSTEIDNKVSEVSIAVATEEAAAHVDRAIAAADIAKNENLE 240
            GFDN GHE D+SV+HNS+VS+ EIDN + EVSIAVA EE  +H DR I+A+DIAKNENL 
Sbjct: 710  GFDNVGHETDDSVDHNSLVSNPEIDNNMLEVSIAVAAEETVSHGDREISASDIAKNENLA 769

Query: 241  VTDVEDQQPAELGASTVNEEREIVSLVDSPAEAGNEKDSKDNFEAREVVDGEVESEPSQE 300
              DVEDQQP                         +EKDSK + + RE + GEVE +PSQE
Sbjct: 770  AMDVEDQQP-------------------------DEKDSKYDSKIREDLPGEVEPKPSQE 829

Query: 301  ATSLVKENIPDNASLEDSRISDAPKIHEPVLNEVDGGKHPLDEEGDIEGSITDGETEGEI 360
            A SLVKE+IPDNAS+ DS ISDAPK  +PVLNEVDG KHPLDEEGDIEGS+TDGETEGEI
Sbjct: 830  ARSLVKESIPDNASVIDSGISDAPKALKPVLNEVDGEKHPLDEEGDIEGSVTDGETEGEI 889

Query: 361  FGSSEAAREFLQELERASGAGSHSGADSSIDHSQRIDGQIVTDSDEADTDDEGDGKQLFD 420
            FGSSEAAREF++ELERASGAG HSGA+SSID SQRIDGQIVTDSDEADTDDEGDGK+LFD
Sbjct: 890  FGSSEAAREFMEELERASGAGFHSGAESSIDQSQRIDGQIVTDSDEADTDDEGDGKELFD 949

Query: 421  SAALAALLKAARDAGSDGGPITVTSQDGSRLFSIERPAGLGSSLTSGKNASRPSRPLSFA 480
            SAALAALLKAARDAGSDGGPITVTSQDGSRLFSIERPAGLGSSL +GKNASRPSRPLSFA
Sbjct: 950  SAALAALLKAARDAGSDGGPITVTSQDGSRLFSIERPAGLGSSLVTGKNASRPSRPLSFA 1009

Query: 481  PSNPR--------------------------------RLGVSPDDSLVAQVLYRFGLVAG 540
            P+NPR                                RLGVSPDDSLVAQVLYRFGLVAG
Sbjct: 1010 PANPRVGDDSENKLSEEEKSKLQKLQQKRVNFLRLVQRLGVSPDDSLVAQVLYRFGLVAG 1069

Query: 541  RSS---------------------------------GKSGVGKSATINSIFGEDKTPINA 600
            RS+                                 GKSGVGKSATINSIFGEDKTPINA
Sbjct: 1070 RSTGQLFSFDNAKNTAMQLEAEGKEDLDFSLNILVLGKSGVGKSATINSIFGEDKTPINA 1129

Query: 601  FGPGTTTVKEIVGTVEGVKIRVFDTPGLRSSSSERGINHKILFSIKNVMKKFPPDIVLYV 660
            FGPGTT+VKEI+GTVEGVKIR+FD+PGLRSSSSER INH+IL SIKNVMKKFPPDIVLYV
Sbjct: 1130 FGPGTTSVKEIIGTVEGVKIRIFDSPGLRSSSSERRINHRILSSIKNVMKKFPPDIVLYV 1189

Query: 661  DRLDNQTRDLNDLLLLRSVSSSLGSSIWKNAIITLTHAASAPPDGPSGSPLGYEVFVAQR 720
            DRLDNQTRDLNDLLLLRSVSS LGSS+WKNAIITLTHAASAPPDGPSGSPLGYEVFVAQR
Sbjct: 1190 DRLDNQTRDLNDLLLLRSVSSYLGSSVWKNAIITLTHAASAPPDGPSGSPLGYEVFVAQR 1249

Query: 721  SHVLQQTVAQAVGDLRMLNPTLMNPVSLVENHPSCRKNRDGQ------------------ 780
            SHVLQQTVAQAVGDLR+LNPTLMNPVSLVENHPSCRKNRDGQ                  
Sbjct: 1250 SHVLQQTVAQAVGDLRILNPTLMNPVSLVENHPSCRKNRDGQKVLPNGQSWRPQLLLLCF 1309

Query: 781  --------------------RKLFGLRARSPPLPYLLSGLLQSRTHPKLSSDQTGENGDS 840
                                RKLFGLR+RSPPLPYLLSGLLQSRTHPKL SDQ G+NGDS
Sbjct: 1310 SIKILAEVGNLSKAPETFDHRKLFGLRSRSPPLPYLLSGLLQSRTHPKLPSDQNGDNGDS 1369

Query: 841  DIDLADLSDSDQEEEEDEYDQLPPFKPLRKSQIAKLSKEQRKAYFEEYDYRVKLLQKKQW 900
            DIDLADLSDSDQEEEEDEYDQLPPFKPLR SQ++KLSKEQRKAYFEEYDYRVKLLQKKQW
Sbjct: 1370 DIDLADLSDSDQEEEEDEYDQLPPFKPLRNSQVSKLSKEQRKAYFEEYDYRVKLLQKKQW 1429

Query: 901  KEELKRMRDIKKKGQPTVDDYGYMGEDDQENGTPAAVQVPLPDMALPPSFDGDNPAYRFR 960
            KEELKRMRD+KK  QPTVDDYGYMGEDDQENG PAAVQVPLPDMALPPSFDGDNPAYRFR
Sbjct: 1430 KEELKRMRDMKKGQQPTVDDYGYMGEDDQENGGPAAVQVPLPDMALPPSFDGDNPAYRFR 1489

Query: 961  FLEPTSQFLARPVLDTHGWDHDCGYDGVNLEHSMAIVSRFPAAVAVQITKDKKEFSIHLD 1006
            FLEPTSQFLARPVLDTHGWDHDCGYDGVNLEHSMAIVSRFPAAVAVQITKDKKEF+IHLD
Sbjct: 1490 FLEPTSQFLARPVLDTHGWDHDCGYDGVNLEHSMAIVSRFPAAVAVQITKDKKEFNIHLD 1549

BLAST of Lag0030990 vs. ExPASy Swiss-Prot
Match: O81283 (Translocase of chloroplast 159, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TOC159 PE=1 SV=1)

HSP 1 Score: 832.4 bits (2149), Expect = 5.5e-240
Identity = 564/1217 (46.34%), Postives = 721/1217 (59.24%), Query Frame = 0

Query: 3    KGSLVAEKQADGEVDLNDKVAAEDGEQLTKLE--------TSSPVEDKAVLGDDE--NSK 62
            K  +V    A+G  +L     AE  E   K +           PV DKA+   +E   S+
Sbjct: 243  KPGVVVVGDAEGSEELKINADAETLEVANKFDQIGDDDSGEFEPVSDKAIEEVEEKFTSE 302

Query: 63   NLEPADGEQEAEMDKGSPVAEKQEEEEIKLNDIVDAEDGERLTKLETGSSF--------- 122
            +   AD  +   +D  +      E E +      + +D E+   LE G ++         
Sbjct: 303  SDSIADSSKLESVDTSA-----VEPEVVAAESGSEPKDVEKANGLEKGMTYAEVIKAASA 362

Query: 123  --DNKADQDDQANSKVSDLADESTGAVLDDK--ILHDSSPVSGTDA-VGNP--EEIKDVE 182
              DN   +++     + D A+E  G  L++K   + DSS +   +  V  P    + DVE
Sbjct: 363  VADNGTKEEESVLGGIVDDAEE--GVKLNNKGDFVVDSSAIEAVNVDVAKPGVVVVGDVE 422

Query: 183  NREIADLVHGAAKLDNGFDNDGH--------EADESVEH---------NSVVSSTEIDNK 242
              E+ +       + N FD  G         E+D++ E          +S+V S+ +D+ 
Sbjct: 423  VSEVLETDGNIPDVHNKFDPIGQGEGGEVELESDKATEEGGGKLVSEGDSMVDSSVVDSV 482

Query: 243  VSEVSIAVATEEAAAHVDRAIAAADIAKNENLEVTDVEDQQPAELGAS-------TVNEE 302
             +++++A            A+   D  K++ ++ T    ++P +L A+        V E 
Sbjct: 483  DADINVAEPGVVVVGAAKEAVIKED-DKDDEVDKTISNIEEPDDLTAAYDGNFELAVKEI 542

Query: 303  REIVSLVDSPAEAGNEKDSKDNFEAREV--VDGEVESEPSQEATSLVKENIPDNASLEDS 362
             E   +     + G E +     E+ +V  VD E +S P+ E+   V++ +  +++ ED 
Sbjct: 543  SEAAKVEPDEPKVGVEVEELPVSESLKVGSVDAEEDSIPAAESQFEVRKVVEGDSAEEDE 602

Query: 363  RISDAPKIHEPVLNEVDGGKHPLDEEGD------IEGSITDGETEGEIFGSSEAAREFLQ 422
                   I      E   G   +D+E        ++GS ++ ETE  IFGSSEAA++FL 
Sbjct: 603  NKLPVEDIVSS--REFSFGGKEVDQEPSGEGVTRVDGSESEEETEEMIFGSSEAAKQFLA 662

Query: 423  ELERA-SGAGSHSG-ADSSIDHSQRIDGQIVTDSDE-ADTDDEGDGKQLFDSAALAALLK 482
            ELE+A SG  +HS  A+ S + S RIDGQIVTDSDE  DT+DEG+ K +FD+AALAALLK
Sbjct: 663  ELEKASSGIEAHSDEANISNNMSDRIDGQIVTDSDEDVDTEDEGEEK-MFDTAALAALLK 722

Query: 483  AARDAG-SDGGPITVTSQDGSRLFSIERPAGLGSSLTSGKNASRP--SRPLSFAPSNP-- 542
            AA   G S+GG  T+TSQDG++LFS++RPAGL SSL   K A+ P  +R   F+ SN   
Sbjct: 723  AATGGGSSEGGNFTITSQDGTKLFSMDRPAGLSSSLRPLKPAAAPRANRSNIFSNSNVTM 782

Query: 543  ------------------------------RRLGVSPDDSLVAQVLYRFGLVAGRSS--- 602
                                          +RLG S +DS+ AQVLYR  L+AGR +   
Sbjct: 783  ADETEINLSEEEKQKLEKLQSLRVKFLRLLQRLGHSAEDSIAAQVLYRLALLAGRQAGQL 842

Query: 603  ------------------------------GKSGVGKSATINSIFGEDKTPINAFGPGTT 662
                                          GK+GVGKSATINSI G     I+AFG  TT
Sbjct: 843  FSLDAAKKKAVESEAEGNEELIFSLNILVLGKAGVGKSATINSILGNQIASIDAFGLSTT 902

Query: 663  TVKEIVGTVEGVKIRVFDTPGLRSSSSERGINHKILFSIKNVMKKFPPDIVLYVDRLDNQ 722
            +V+EI GTV GVKI   DTPGL+S++ ++  N K+L S+K VMKK PPDIVLYVDRLD Q
Sbjct: 903  SVREISGTVNGVKITFIDTPGLKSAAMDQSTNAKMLSSVKKVMKKCPPDIVLYVDRLDTQ 962

Query: 723  TRDLNDLLLLRSVSSSLGSSIWKNAIITLTHAASAPPDGPSGSPLGYEVFVAQRSHVLQQ 782
            TRDLN+L LLR++++SLG+SIWKNAI+TLTHAASAPPDGPSG+PL Y+VFVAQ SH++QQ
Sbjct: 963  TRDLNNLPLLRTITASLGTSIWKNAIVTLTHAASAPPDGPSGTPLSYDVFVAQCSHIVQQ 1022

Query: 783  TVAQAVGDLRMLNPTLMNPVSLVENHPSCRKNRDG------------------------- 842
            ++ QAVGDLR++NP+LMNPVSLVENHP CRKNR+G                         
Sbjct: 1023 SIGQAVGDLRLMNPSLMNPVSLVENHPLCRKNREGVKVLPNGQTWRSQLLLLCYSLKVLS 1082

Query: 843  -------------QRKLFGLRARSPPLPYLLSGLLQSRTHPKLSSDQTGENGDSDIDLAD 902
                          RK+FG R RSPPLPYLLS LLQSR HPKL  DQ G++ DSDI++ D
Sbjct: 1083 ETNSLLRPQEPLDHRKVFGFRVRSPPLPYLLSWLLQSRAHPKLPGDQGGDSVDSDIEIDD 1142

Query: 903  LSDSDQEE-EEDEYDQLPPFKPLRKSQIAKLSKEQRKAYFEEYDYRVKLLQKKQWKEELK 962
            +SDS+QE+ E+DEYDQLPPFKPLRK+Q+AKLS EQRKAYFEEYDYRVKLLQKKQW+EELK
Sbjct: 1143 VSDSEQEDGEDDEYDQLPPFKPLRKTQLAKLSNEQRKAYFEEYDYRVKLLQKKQWREELK 1202

Query: 963  RMRDIKKKGQPTVD-DYGYMG-EDDQENGTPAAVQVPLPDMALPPSFDGDNPAYRFRFLE 1006
            RM+++KK G+   + ++GY G EDD ENG PAAV VPLPDM LPPSFD DN AYR+R+LE
Sbjct: 1203 RMKEMKKNGKKLGESEFGYPGEEDDPENGAPAAVPVPLPDMVLPPSFDSDNSAYRYRYLE 1262

BLAST of Lag0030990 vs. ExPASy Swiss-Prot
Match: A9SV59 (Translocase of chloroplast 101, chloroplastic OS=Physcomitrium patens OX=3218 GN=TOC101 PE=3 SV=1)

HSP 1 Score: 500.0 bits (1286), Expect = 6.5e-140
Identity = 333/880 (37.84%), Postives = 462/880 (52.50%), Query Frame = 0

Query: 276  EAREVVDGEVESEPSQEATSLVKENIPDNASLEDSRISDAPKIHEPVLNEVDGGKHPLDE 335
            E R++      SE      +LV E  P+   +E   +  +P      L  VD    PL  
Sbjct: 15   ELRKLSASRSLSEEVGVDPALVSEGAPEGV-IEGPAVVSSPAKMYTALKAVDDEMPPLKS 74

Query: 336  EGDIEGSITDGETEGEIFGSSEAAREFLQELERASGAGSHSGADSSIDHSQRIDGQIVTD 395
            E          E++   F + + A E                 DS  D     D     D
Sbjct: 75   ENKAVVETEKVESKPRGFSAIDFAEE---------------DGDSDADAEDEDDED---D 134

Query: 396  SDEADTDDEGDGKQLFDSAALAALLKAARDAGSDG--GP--------------ITVTSQD 455
             D+ + DD+ D K +  + ALA L  A+    S G  GP                 T+ D
Sbjct: 135  EDDDEDDDDEDDKDMVTAKALAELANASGKKSSMGAAGPSLPSLPQRPAVRKTAAATALD 194

Query: 456  GSRLFSIERPAGLGS---SLTSGKNASRPSRPLSFAPSN-----------PRRLGVSPDD 515
             +   + +RP G  S   + T+ +NA+  +   +                  RLG SP +
Sbjct: 195  TAGRIT-QRPNGAPSTQLTATTEENANSDTAEGNETREKLQNIRVKFLRLAHRLGQSPQN 254

Query: 516  SLVAQVLYRFGLV-----------AGRSS-----------------------------GK 575
             +VAQVLYR GL            AG  S                             GK
Sbjct: 255  VVVAQVLYRLGLAESLRGGNTSNRAGAFSFDRANALAEEQEAANQEEELDFACTILVLGK 314

Query: 576  SGVGKSATINSIFGEDKTPINAFGPGTTTVKEIVGTVEGVKIRVFDTPGLRSSSSERGIN 635
            +GVGKSATINSIF + K+  +AF P T  V+EIVGTV G+K+RV DTPGL  S +++  N
Sbjct: 315  TGVGKSATINSIFDDRKSVTSAFKPSTNKVQEIVGTVHGIKVRVIDTPGLLPSVADQQHN 374

Query: 636  HKILFSIKNVMKKFPPDIVLYVDRLDNQTRDLNDLLLLRSVSSSLGSSIWKNAIITLTHA 695
             +I+  +K  +KK  PDIVLY DRLD Q+RD  DL LL++++   G+++W NAI+ LTHA
Sbjct: 375  ERIMGQVKKHIKKASPDIVLYFDRLDMQSRDFGDLPLLKTITDLFGAAVWFNAIVVLTHA 434

Query: 696  ASAPPDGPSGSPLGYEVFVAQRSHVLQQTVAQAVGDLRMLNPTLMNPVSLVENHPSCRKN 755
            +SAPPDGP+G PL YE+FVAQRSHV+QQT+ QA GD+R     LMNPVSLVENHP+CR N
Sbjct: 435  SSAPPDGPNGVPLSYEMFVAQRSHVVQQTIRQAAGDMR-----LMNPVSLVENHPACRTN 494

Query: 756  RDGQRKL--------------------------------------FGLRARSPPLPYLLS 815
            R+GQR L                                      FG R+R PPLP+LLS
Sbjct: 495  RNGQRVLPNGQIWKPQLLLLCFASKILAEANSLLKLQETATPGRPFGQRSRVPPLPFLLS 554

Query: 816  GLLQSRTHPKLSSDQTGENGDSDIDLADLSDSDQEEEEDEYDQLPPFKPLRKSQIAKLSK 875
             LLQSR   KL  +Q  E+ +SD D     + +++ E D+YD+LPPF+PL K ++ +L+K
Sbjct: 555  SLLQSRAQLKLPDEQLDESDESDDD-----EEEEDSEADDYDELPPFRPLSKEELEELTK 614

Query: 876  EQRKAYFEEYDYRVKLLQKKQWKEELKRMRDIKKK-GQPTVDDYGYMGEDDQENGTPAAV 935
            EQR+ Y +E   R +L QKKQ++EE++R +++KK+  Q + ++     E D E G PAAV
Sbjct: 615  EQRQDYMDELADRERLFQKKQYREEMRRRKEMKKRQAQMSKEELAQPDEADDEAGQPAAV 674

Query: 936  QVPLPDMALPPSFDGDNPAYRFRFLEPTSQFLARPVLDTHGWDHDCGYDGVNLEHSMAIV 995
             VP+PDMALPPSFD DNP +R+R+LE  +Q+L RPVL+THGWDHD GYDG N+E    + 
Sbjct: 675  PVPMPDMALPPSFDSDNPTHRYRYLETANQWLVRPVLETHGWDHDAGYDGFNVEKMFVVK 734

Query: 996  SRFPAAVAVQITKDKKEFSIHLDSSVSAKHGENGSTMAGF-------------------- 1003
            ++ PA+++ Q+TKDKKE  ++ +++ S KHGE   T+ GF                    
Sbjct: 735  NKIPASISGQVTKDKKESQVNFEAAASLKHGEGKVTLTGFDVQTIGKDLAYTLRAETRFN 794

BLAST of Lag0030990 vs. ExPASy Swiss-Prot
Match: A9SY65 (Translocase of chloroplast 108, chloroplastic OS=Physcomitrium patens OX=3218 GN=TOC108 PE=3 SV=1)

HSP 1 Score: 495.0 bits (1273), Expect = 2.1e-138
Identity = 352/998 (35.27%), Postives = 499/998 (50.00%), Query Frame = 0

Query: 167  AAKLDNGFDNDGHEADESVEHNSVVSSTEIDNKVSEVSIAVATEEAAAHVDRAIAAADIA 226
            AA+L+    N+       V  +  V     +  V   SI+    E+ +  +   A     
Sbjct: 9    AARLEKEASNNTVNPVREVSEDD-VKDVSGETTVVTTSISEGANESLSKKEDEPALIGSN 68

Query: 227  KNENLEVTDVEDQQPAELGASTVNEEREIVSLVDSPAEAGNEKDSKDNFEAREVVDGEVE 286
              E LE   +E Q       S +  + E VS   +P+ A  EK+S +  E R V +G++E
Sbjct: 69   VPEELEGNSLEVQ-------SAITTDLEKVSSTPTPSNA--EKESPEATEVRIVEEGKLE 128

Query: 287  -SEPSQEATSLVKENIPDNASLEDSRISDAPKIHEPVLNEVDGGKHPLDEEGDIEGSITD 346
             ++PS     L KE       LED  +  +P      L  VDG    L  E   +G  TD
Sbjct: 129  KADPSVVNEELSKE------ILEDPEVVPSPAKMYTALKAVDGDMPVLKSENGNDGD-TD 188

Query: 347  GETEGEIFGSSEAAREFLQELERASGAGSHSGADSSIDHSQRIDGQIVTDSDEADTDDEG 406
              T  E                                     D +   D D+ D D++ 
Sbjct: 189  ANTADE-------------------------------------DNE--NDEDDVDEDEDE 248

Query: 407  DGKQLFDSAALAALLKAARDAGSDGGPITVTSQDGS--------RLFSIERPAGLGSSLT 466
            D   +  + ALA L   A  +G+     T  S   +        +  ++ +P    +S +
Sbjct: 249  DDADMDTAKALAELAMTAGKSGNPAFSGTKPSMGAAGPSLPSLPQRPAVRKPIAATASDS 308

Query: 467  SGKNASRPSRPLSFAPSN------------------------------PRRLGVSPDDSL 526
             G+N  RP+  LS   ++                                RLG SP + +
Sbjct: 309  PGRNTQRPNGALSTQITSTTDESASSDAAEGDETREKLQNIRVKFLRLAHRLGQSPQNVV 368

Query: 527  VAQVLYRFGL---VAGRSS-------------------------------------GKSG 586
            VAQVLYR GL   + G S+                                     GK+G
Sbjct: 369  VAQVLYRLGLAESLRGGSAPNRSGAFSFDRANALAEEQEAANQEEELDFACTILVLGKTG 428

Query: 587  VGKSATINSIFGEDKTPINAFGPGTTTVKEIVGTVEGVKIRVFDTPGLRSSSSERGINHK 646
            VGKS+TINSIF E K+  +AF P T  V+E++GTV G+K+RV DTPGL  S +++  N +
Sbjct: 429  VGKSSTINSIFDERKSVTSAFKPSTNKVQEVIGTVHGIKVRVIDTPGLLPSVADQQHNER 488

Query: 647  ILFSIKNVMKKFPPDIVLYVDRLDNQTRDLNDLLLLRSVSSSLGSSIWKNAIITLTHAAS 706
            I+  +K  +KK  PDIVLY DRLD Q+RD  DL LLR+++   G+++W NAI+ LTHA+S
Sbjct: 489  IMGQVKKYIKKASPDIVLYFDRLDMQSRDFGDLPLLRTITDLFGAAVWFNAIVVLTHASS 548

Query: 707  APPDGPSGSPLGYEVFVAQRSHVLQQTVAQAVGDLRMLNPTLMNPVSLVENHPSCRKNRD 766
            APPDGP+G PL YE+FVAQRSHV+QQT+ QA GD+R     LMNPVSLVENHP+CR NR 
Sbjct: 549  APPDGPNGVPLSYEMFVAQRSHVVQQTIRQAAGDMR-----LMNPVSLVENHPACRTNRT 608

Query: 767  GQRKL--------------------------------------FGLRARSPPLPYLLSGL 826
            GQR L                                      FG R+R PPLP+LLS L
Sbjct: 609  GQRVLPNGQIWKPQLLLLCFASKILAEANSLLKLQETTAPGRPFGQRSRVPPLPFLLSSL 668

Query: 827  LQSRTHPKLSSDQTGENGDSDIDLADLSDSDQEEEEDEYDQLPPFKPLRKSQIAKLSKEQ 886
            LQSR   KL  +Q GE+ +SD D     + +++ + D+YD+LPPF+PL K ++  L+KEQ
Sbjct: 669  LQSRAQLKLPDEQAGESDESDDD-----EEEEDSDADDYDELPPFRPLSKEELEDLTKEQ 728

Query: 887  RKAYFEEYDYRVKLLQKKQWKEELKRMRDIKKK-GQPTVDDYGYMGEDDQENGTPAAVQV 946
            R+ Y EE   R ++ QKKQ++EE++R ++ KK+  Q + ++     E + E G  AAV V
Sbjct: 729  REDYMEELADRERMFQKKQYREEIRRRKEAKKRQAQMSKEELAEAEEAEDEAGNAAAVPV 788

Query: 947  PLPDMALPPSFDGDNPAYRFRFLEPTSQFLARPVLDTHGWDHDCGYDGVNLEHSMAIVSR 1003
            P+PDMALPPSFD DNP +R+R+LE  +Q+L RPVL+THGWDHD GYDG N+E    +  +
Sbjct: 789  PMPDMALPPSFDSDNPTHRYRYLETANQWLVRPVLETHGWDHDAGYDGFNVEKMFVVKEK 848

BLAST of Lag0030990 vs. ExPASy Swiss-Prot
Match: Q9SLF3 (Translocase of chloroplast 132, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TOC132 PE=1 SV=1)

HSP 1 Score: 489.2 bits (1258), Expect = 1.1e-136
Identity = 285/644 (44.25%), Postives = 371/644 (57.61%), Query Frame = 0

Query: 478  RLGVSPDDSLVAQVLYRFGL---VAGRSS------------------------------- 537
            RLG +P + +VAQVLYR GL   + GR+                                
Sbjct: 517  RLGQTPHNVVVAQVLYRLGLAEQLRGRNGSRVGAFSFDRASAMAEQLEAAGQDPLDFSCT 576

Query: 538  ----GKSGVGKSATINSIFGEDKTPINAFGPGTTTVKEIVGTVEGVKIRVFDTPGLRSSS 597
                GKSGVGKSATINSIF E K   +AF  GT  V+++ G V+G+K+RV DTPGL  S 
Sbjct: 577  IMVLGKSGVGKSATINSIFDEVKFCTDAFQMGTKRVQDVEGLVQGIKVRVIDTPGLLPSW 636

Query: 598  SERGINHKILFSIKNVMKKFPPDIVLYVDRLDNQTRDLNDLLLLRSVSSSLGSSIWKNAI 657
            S++  N KIL S+K  +KK PPDIVLY+DRLD Q+RD  D+ LLR++S   G SIW NAI
Sbjct: 637  SDQAKNEKILNSVKAFIKKNPPDIVLYLDRLDMQSRDSGDMPLLRTISDVFGPSIWFNAI 696

Query: 658  ITLTHAASAPPDGPSGSPLGYEVFVAQRSHVLQQTVAQAVGDLRMLNPTLMNPVSLVENH 717
            + LTHAAS PPDGP+G+   Y++FV QRSHV+QQ + QA GD+R     LMNPVSLVENH
Sbjct: 697  VGLTHAASVPPDGPNGTASSYDMFVTQRSHVIQQAIRQAAGDMR-----LMNPVSLVENH 756

Query: 718  PSCRKNRDGQRKL-------------------------------------FGLRARSPPL 777
             +CR NR GQR L                                     F  R+++PPL
Sbjct: 757  SACRTNRAGQRVLPNGQVWKPHLLLLSFASKILAEANALLKLQDNIPGRPFAARSKAPPL 816

Query: 778  PYLLSGLLQSRTHPKLSSDQTGENGDSDIDLADLSDSDQEEEEDEYDQLPPFKPLRKSQI 837
            P+LLS LLQSR  PKL   Q G+  D D    DL +S   +EE EYDQLPPFK L K+Q+
Sbjct: 817  PFLLSSLLQSRPQPKLPEQQYGDEEDED----DLEESSDSDEESEYDQLPPFKSLTKAQM 876

Query: 838  AKLSKEQRKAYFEEYDYRVKLLQKKQWKEELKRMRDIKKKGQPTVD-DYGYMGEDDQENG 897
            A LSK Q+K Y +E +YR KLL KKQ KEE KR +  KK      D   GY    ++E+G
Sbjct: 877  ATLSKSQKKQYLDEMEYREKLLMKKQMKEERKRRKMFKKFAAEIKDLPDGYSENVEEESG 936

Query: 898  TPAAVQVPLPDMALPPSFDGDNPAYRFRFLEPTSQFLARPVLDTHGWDHDCGYDGVNLEH 957
             PA+V VP+PD++LP SFD DNP +R+R+L+ ++Q+L RPVL+THGWDHD GY+GVN E 
Sbjct: 937  GPASVPVPMPDLSLPASFDSDNPTHRYRYLDSSNQWLVRPVLETHGWDHDIGYEGVNAER 996

Query: 958  SMAIVSRFPAAVAVQITKDKKEFSIHLDSSVSAKHGENGSTMAGF--------------- 1002
               +  + P +V+ Q+TKDKK+ ++ L+ + S KHGE  ST  GF               
Sbjct: 997  LFVVKEKIPISVSGQVTKDKKDANVQLEMASSVKHGEGKSTSLGFDMQTVGKELAYTLRS 1056

BLAST of Lag0030990 vs. ExPASy Swiss-Prot
Match: A9SV60 (Translocase of chloroplast 126, chloroplastic OS=Physcomitrium patens OX=3218 GN=TOC126 PE=3 SV=1)

HSP 1 Score: 479.9 bits (1234), Expect = 6.9e-134
Identity = 359/1046 (34.32%), Postives = 518/1046 (49.52%), Query Frame = 0

Query: 139  PVSGTDAVGNPEEI-----KDVENREIADLVHGAAKLDNG-FDNDGHEADESVEHNSVVS 198
            P S   +VG   +      +D+E R   + ++     D+G  + DG E D S   +S  S
Sbjct: 101  PPSPVGSVGEQSQFAGGVSEDLEERGQEEYLYYDDYGDDGEVEKDGSEKD-STSSSSSSS 160

Query: 199  STEIDNKVSEVSIAVATEEAAAHVDRAIAAADIAKNENLEVTDVEDQQPAELGASTVNEE 258
            S+E  +  S     +   E  A  +RA+  A+ +      VTD E++   EL     N E
Sbjct: 161  SSECSSSASNTEDEMDISEYGASSERAMPLANPS-----GVTDEEEEDGKEL---KYNVE 220

Query: 259  REIVSLVDSP------------------AEAGNE-KDSKDNFEAREVVDGEVESEPSQEA 318
            R + +  + P                  AE GN  KDS++        D EV+ E ++ +
Sbjct: 221  RAVTAEENMPNGLKLGSEARGIASSSRGAELGNAFKDSRE--------DHEVQEELTERS 280

Query: 319  TSLVKENIPDNASLEDSRISDAPKIHEPVLNEVDGGKHPLDEEGDIEGSITDGETEGEIF 378
              +  EN       ++   +D+ +I +    E+   +  +  E       ++      + 
Sbjct: 281  VKVAVENYD-----QEGEDADSTEIKKEFPRELTQSRTVI--ESPAYRFTSEPVDPALLE 340

Query: 379  GSSEAAREFLQELERASGAGSHSGADSSIDHSQRIDGQIVTDSDEADTDDEGDGKQLFDS 438
              SE A+   Q   R +   S + AD+  D           D D    D+  DG    + 
Sbjct: 341  LKSEKAQPNTQSFARIAEGESDADADADAD-----------DEDVESGDEHEDGYTEINI 400

Query: 439  AALAALLKAARDAGSDG--GPITVTSQDGSRLFSI------ERPAGLGSSLTSGKNASRP 498
               A   ++  ++G++   GP       G  L S+       RPA   ++ T   NA+  
Sbjct: 401  RQAAGKSESENESGNNPSLGPA------GPSLISVLVRKTARRPASTAATDTQSSNAASS 460

Query: 499  SRPLSFAPSNP-------------------------RRLGVSPDDSLVAQVLYRFGL--- 558
            ++       NP                          RLG SP + +VAQVLYR GL   
Sbjct: 461  TQVAGTTDVNPSIEVNEVNETREKLQNIRVKFLRLVHRLGQSPQNVVVAQVLYRLGLAES 520

Query: 559  VAGRSS-------------------------------------GKSGVGKSATINSIFGE 618
            + G S+                                     GK+GVGKSATINSIF E
Sbjct: 521  LRGGSTRNHTRAFDFDRANAIAEEQEADNQEEELDFACTILVLGKTGVGKSATINSIFDE 580

Query: 619  DKTPINAFGPGTTTVKEIVGTVEGVKIRVFDTPGLRSSSSERGINHKILFSIKNVMKKFP 678
             K+  NA+ P TT V E+VGT+ GVK+R  DTPGL  S +++  N +I+  +K  +KK  
Sbjct: 581  HKSVTNAYNPSTTNVYEVVGTMLGVKVRFVDTPGLLFSVADQRHNERIMGRVKKYIKKAS 640

Query: 679  PDIVLYVDRLDNQTRDLNDLLLLRSVSSSLGSSIWKNAIITLTHAASAPPDGPSGSPLGY 738
            PDIVLY DR+D QTR+  D+ LLR++++  G+++W N I+ LTHA++APPDGP+G+P+GY
Sbjct: 641  PDIVLYFDRMDMQTREFGDVPLLRTITNVFGTAVWFNTIVVLTHASTAPPDGPNGTPMGY 700

Query: 739  EVFVAQRSHVLQQTVAQAVGDLRMLNPTLMNPVSLVENHPSCRKNRDGQRKL-------- 798
            E+FVAQRSH +QQ++ Q  GD+R     L NPVSLVENHP+CR NR+GQR L        
Sbjct: 701  ELFVAQRSHSVQQSIRQVAGDMR-----LQNPVSLVENHPACRANRNGQRVLPNGQIWKP 760

Query: 799  ------------------------------FGLRARSPPLPYLLSGLLQSRTHPKLSSDQ 858
                                          FG R+R PPLP+LLS LLQSR   KL  +Q
Sbjct: 761  HLMLLCFASKILAEANTLLKLQDTAAPGRPFGQRSRVPPLPFLLSSLLQSRAQLKLPDEQ 820

Query: 859  TGENGDSDIDLADLSDSDQEEEEDEYDQLPPFKPLRKSQIAKLSKEQRKAYFEEYDYRVK 918
              E+ +SD       D + EEE DEYD LPPF+ L K ++ +LSK+QR+ Y EE   R +
Sbjct: 821  LDESDESD------DDEEDEEEGDEYDDLPPFRSLSKEELEELSKDQRQEYAEELAVRER 880

Query: 919  LLQKKQWKEELKRMRDIKKKGQPTVDD--YGYMGEDDQENGTPAAVQVPLPDMALPPSFD 978
            L QKKQ +E+L+R +++KK+      +       E D E G PAAV VP+PDMALPPSFD
Sbjct: 881  LFQKKQHREQLQRRKEMKKRATAMRKEGLSHPADEADDEAGQPAAVPVPMPDMALPPSFD 940

Query: 979  GDNPAYRFRFLEPTSQFLARPVLDTHGWDHDCGYDGVNLEHSMAIVSRFPAAVAVQITKD 1003
             DNP +R+R+LE  +Q+L RPVL+THGWDHD GYDG N+E    + ++ PA+++ Q+TKD
Sbjct: 941  SDNPTHRYRYLETANQWLVRPVLETHGWDHDAGYDGFNVEKMFVVKNKIPASISGQVTKD 1000

BLAST of Lag0030990 vs. ExPASy TrEMBL
Match: A0A6J1CNN0 (translocase of chloroplast 159, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111013263 PE=3 SV=1)

HSP 1 Score: 1530.8 bits (3962), Expect = 0.0e+00
Identity = 857/1147 (74.72%), Postives = 908/1147 (79.16%), Query Frame = 0

Query: 9    EKQADGEVDLNDKVAAEDGEQLTKLETSSPVEDKAVLGDDENSKNLEPADGEQEAEMDKG 68
            E+QADGE++LN+KV+AEDGEQL +LET SPVEDKAVL D+EN K LEPADG QE EM+KG
Sbjct: 317  ERQADGEIELNEKVSAEDGEQLKELETGSPVEDKAVLDDNENFKVLEPADGGQEVEMEKG 376

Query: 69   SPVAEKQEEEEIKLNDIVDAEDGERLTKLETGSSFDNKADQDDQANSKVSDLADESTGAV 128
            SPVAEK+ +   K ND ++AEDGE+L KLE GSS  NKAD DDQANSKVS+LADE +   
Sbjct: 377  SPVAEKEADGGTKFNDKMNAEDGEQLEKLEIGSSVVNKADLDDQANSKVSELADEFSSGC 436

Query: 129  LDDKILHDSSPVSGTDAVGNPEEIKDVENREIADLVHGAAKLDNGFDNDGHEADESVEHN 188
            LDDK LH+SS VSGTDAVGN EEIKDV NR   DLV    KLDNGFDN GHEADE V+HN
Sbjct: 437  LDDKTLHESSLVSGTDAVGNLEEIKDVGNRVSVDLVREGTKLDNGFDNVGHEADEFVDHN 496

Query: 189  SVVSSTEIDNKVSEVSIAVATEEAAAHVDRAIAAADIAKNENLEVTDVEDQQPAELGAST 248
            SVVS++EIDNK  E+SI VATEEA  H DR  AA DIA +E L   +VEDQQP +     
Sbjct: 497  SVVSNSEIDNKEPEISIPVATEEAVLHGDRGFAATDIANHEKLAAKNVEDQQPDD----- 556

Query: 249  VNEEREIVSLVDSPAEAGNEKDSKDNFEAREVVDGEVESEPSQEATSLVKENIPDNASLE 308
                RE VSL+DSP EAGNEKDSKD+ + RE+V GEVESE SQ ATSLVKE IPD+A++E
Sbjct: 557  --GLREAVSLLDSPHEAGNEKDSKDDSKIREIVAGEVESESSQGATSLVKETIPDSATVE 616

Query: 309  DSRISDAPKIHEPVLNEVDGGKHPLDEEGDIEGSITDGETEGEIFGSSEAAREFLQELER 368
            DS+IS  PKI EPVLNEVDG K+  DEEGD EGS+TDGETEGEIFGSSEAAR+FLQELER
Sbjct: 617  DSKISATPKIIEPVLNEVDGEKYHSDEEGDGEGSVTDGETEGEIFGSSEAARQFLQELER 676

Query: 369  ASGAGSHSGADSSIDHSQRIDGQIVTDSDEADTDDEGDGKQLFDSAALAALLKAARDAGS 428
            ASGAGSHSGA+SSIDHS RIDGQIVTDSDEADT+DEG+GK++FDSAALAALLKAARDAGS
Sbjct: 677  ASGAGSHSGAESSIDHSHRIDGQIVTDSDEADTEDEGEGKEMFDSAALAALLKAARDAGS 736

Query: 429  DGGPITVTSQDGSRLFSIER---PAGLGSSLTSGKNASRPSRPLSFAPSNPR-------- 488
            DGGPITVTSQDGSRLFSIER   PAGLGSSLTSGKNASRPSRPLSFA SNPR        
Sbjct: 737  DGGPITVTSQDGSRLFSIERPAGPAGLGSSLTSGKNASRPSRPLSFASSNPRVGDDSENR 796

Query: 489  ------------------------RLGVSPDDSLVAQVLYRFGLVAGRSS---------- 548
                                    RLGVSPDDSLVAQVLYRFGLVAGR++          
Sbjct: 797  LSEEEKNKLQKLQQIRVNFLRLVQRLGVSPDDSLVAQVLYRFGLVAGRATGQLFSFDNAK 856

Query: 549  -----------------------GKSGVGKSATINSIFGEDKTPINAFGPGTTTVKEIVG 608
                                   GK+GVGKSATINSIFGEDKTPINAFGPGTTTVKEIVG
Sbjct: 857  TTAIQLEAEGKEDLDFSLNILVIGKTGVGKSATINSIFGEDKTPINAFGPGTTTVKEIVG 916

Query: 609  TVEGVKIRVFDTPGLRSSSSERGINHKILFSIKNVMKKFPPDIVLYVDRLDNQTRDLNDL 668
            T+EGVKIRVFD+PGLRSSSSER INHKIL SIKNVMKK PPDIVLYVDRLDNQTRDLNDL
Sbjct: 917  TIEGVKIRVFDSPGLRSSSSERSINHKILSSIKNVMKKSPPDIVLYVDRLDNQTRDLNDL 976

Query: 669  LLLRSVSSSLGSSIWKNAIITLTHAASAPPDGPSGSPLGYEVFVAQRSHVLQQTVAQAVG 728
            LLLRS+SSSLGSSIWKNAIITLTH ASAPPDGPSG PLGYEVFVAQRSHVLQQTVAQAVG
Sbjct: 977  LLLRSISSSLGSSIWKNAIITLTHGASAPPDGPSGVPLGYEVFVAQRSHVLQQTVAQAVG 1036

Query: 729  DLRMLNPTLMNPVSLVENHPSCRKNRDGQ------------------------------- 788
            DLR+LNPTLMNPVSLVENHPSCRKNRDGQ                               
Sbjct: 1037 DLRILNPTLMNPVSLVENHPSCRKNRDGQKVLPNGQSWRPQLLLLCFSIKILAEVGSLSK 1096

Query: 789  -------RKLFGLRARSPPLPYLLSGLLQSRTHPKLSSDQTGENGDSDIDLADLSDSDQE 848
                   RKLFGLRARSPPLPYLLSGLLQSR HPKLSSDQ G+NGDSDIDLADLSDSDQE
Sbjct: 1097 APETFDHRKLFGLRARSPPLPYLLSGLLQSRAHPKLSSDQAGDNGDSDIDLADLSDSDQE 1156

Query: 849  EEEDEYDQLPPFKPLRKSQIAKLSKEQRKAYFEEYDYRVKLLQKKQWKEELKRMRDIKKK 908
            EEEDEYDQLPPFKPLRKSQIAKLSKEQR+AYFEEYDYRVKLLQKKQWKEELKRMRDIKKK
Sbjct: 1157 EEEDEYDQLPPFKPLRKSQIAKLSKEQRRAYFEEYDYRVKLLQKKQWKEELKRMRDIKKK 1216

Query: 909  GQPTVDDYGYMGEDDQENGTPAAVQVPLPDMALPPSFDGDNPAYRFRFLEPTSQFLARPV 968
            GQ   DDYGYMGEDDQENGTPAAVQVPLPDMALPPSFDGDNPAY+FRFLEPTSQFLARPV
Sbjct: 1217 GQLPADDYGYMGEDDQENGTPAAVQVPLPDMALPPSFDGDNPAYQFRFLEPTSQFLARPV 1276

Query: 969  LDTHGWDHDCGYDGVNLEHSMAIVSRFPAAVAVQITKDKKEFSIHLDSSVSAKHGENGST 1006
            LDTHGWDHDCGYDGVNLEHSMAIVSRFPAAVAVQITKDKKEF+IHLDSSVSAKHGENGST
Sbjct: 1277 LDTHGWDHDCGYDGVNLEHSMAIVSRFPAAVAVQITKDKKEFNIHLDSSVSAKHGENGST 1336

BLAST of Lag0030990 vs. ExPASy TrEMBL
Match: A0A0A0KT42 (Chloroplast protein import component Toc159 OS=Cucumis sativus OX=3659 GN=Csa_4G001670 PE=3 SV=1)

HSP 1 Score: 1518.4 bits (3930), Expect = 0.0e+00
Identity = 848/1157 (73.29%), Postives = 912/1157 (78.82%), Query Frame = 0

Query: 2    EKGSLVAEKQADGEVDLNDKV-AAEDGEQLTKLETSSPVEDKAVLGDDENSKNLEPADGE 61
            ++ +LVAEKQ D EV LND V AA+DGEQL  LET SPV++K VL DDENS  LEPADG 
Sbjct: 337  QEETLVAEKQVDVEVKLNDTVAAADDGEQLKNLETDSPVDNKIVLADDENSGVLEPADGG 396

Query: 62   QEAEMDKGSPVAEKQEEEEIKLNDIVDAEDGERLTKLETGSSFDNKADQDDQANSKVSDL 121
            QEAEM KGSPVAE Q + EI+L   VDAEDGE LTKLE  S  DNK              
Sbjct: 397  QEAEMAKGSPVAEMQADGEIRLKGKVDAEDGELLTKLEPVSFADNK-------------- 456

Query: 122  ADESTGAVLDDKILHDSSPVSGTDAVGNPEEIKDVENREIADLVHGAAKLDNGFDNDGHE 181
             DE T + LDDK LH+SS VS TD +GNPEEIKD+EN+E A+L HGA KLDNGFD+ GHE
Sbjct: 457  TDEFTASALDDKTLHESSQVSATDVLGNPEEIKDLENKETANLAHGATKLDNGFDSVGHE 516

Query: 182  ADESVEHNSVVSSTEIDNKVSEVSIAVATEEAAAHVDRAIAAADIAKNENLEVTDVEDQQ 241
             ++ V+ +SVV ++E+DN +   +IAV TEE   H +RAIAA+DIAK+ENL VTDVEDQQ
Sbjct: 517  VNQPVDSDSVVLNSEVDNSMPGANIAVGTEETEPHGNRAIAASDIAKSENLAVTDVEDQQ 576

Query: 242  PAELGASTVNEEREIVSLVDSPAEAGNEKDSKDNFEAREVVDGEVESEPSQEATSLVKEN 301
               +GASTVNEERE V+L DSP++AGNEKDSKD+ + RE V G+VESEPSQE   L+KE+
Sbjct: 577  LDGVGASTVNEERETVNLADSPSKAGNEKDSKDDSKIREDVPGDVESEPSQEDRGLIKES 636

Query: 302  IPDNASLEDSRISDAPKIHEPVLNEVDGGKHPLDEEGDIEGSITDGETEGEIFGSSEAAR 361
            IPDNAS++DS ISDAPK+ EPVL+EVDG KHPLDEEGDIEGS TDGETE EIFGSSEAAR
Sbjct: 637  IPDNASVKDSGISDAPKLLEPVLSEVDGEKHPLDEEGDIEGSGTDGETEAEIFGSSEAAR 696

Query: 362  EFLQELERASGAGSHSGADSSIDHSQRIDGQIVTDSDEADTDDEGDGKQLFDSAALAALL 421
            EFLQELERASGAGSHSGA+SSIDHSQRIDGQIVTDSDEADT+DEGDGK+LFDSAALAALL
Sbjct: 697  EFLQELERASGAGSHSGAESSIDHSQRIDGQIVTDSDEADTEDEGDGKELFDSAALAALL 756

Query: 422  KAARDAGSDGGPITVTSQDGSRLFSIERPAGLGSSLTSGKNASRPSRPLSFAPSNPR--- 481
            KAARDAGSDGGPITVT+QDGSRLFSIERPAGLGSSL SGKNASRPSRPL+FA SNPR   
Sbjct: 757  KAARDAGSDGGPITVTTQDGSRLFSIERPAGLGSSLISGKNASRPSRPLTFASSNPRVGD 816

Query: 482  -----------------------------RLGVSPDDSLVAQVLYRFGLVAGRSS----- 541
                                         RLGVSPDDSLVAQVLYRFGLVAGRS+     
Sbjct: 817  DAENKLSEEEKTKLQKLQKIRVNFLRLVQRLGVSPDDSLVAQVLYRFGLVAGRSTGQLFS 876

Query: 542  ----------------------------GKSGVGKSATINSIFGEDKTPINAFGPGTTTV 601
                                        GKSGVGKSATINSIFGE+KTPINAFGPGTTTV
Sbjct: 877  FDNAKNTAIQLEAEGKEDLDFSLNILVLGKSGVGKSATINSIFGENKTPINAFGPGTTTV 936

Query: 602  KEIVGTVEGVKIRVFDTPGLRSSSSERGINHKILFSIKNVMKKFPPDIVLYVDRLDNQTR 661
            KEI+GTVEGVKIRVFD+PGLRSSSSER IN++IL SIKNVMKKFPPDIVLYVDRLDNQTR
Sbjct: 937  KEIIGTVEGVKIRVFDSPGLRSSSSERRINNRILSSIKNVMKKFPPDIVLYVDRLDNQTR 996

Query: 662  DLNDLLLLRSVSSSLGSSIWKNAIITLTHAASAPPDGPSGSPLGYEVFVAQRSHVLQQTV 721
            DLNDLLLLRSVSSSLGSSIWKNAIITLTHAASAPPDGPSGSPLGYEVFVAQRSHVLQQTV
Sbjct: 997  DLNDLLLLRSVSSSLGSSIWKNAIITLTHAASAPPDGPSGSPLGYEVFVAQRSHVLQQTV 1056

Query: 722  AQAVGDLRMLNPTLMNPVSLVENHPSCRKNRDGQ-------------------------- 781
            AQAVGDLR+LNPTLMNPVSLVENHPSCRKNRDGQ                          
Sbjct: 1057 AQAVGDLRILNPTLMNPVSLVENHPSCRKNRDGQKVLPNGQTWRPQLLLLCFSIKILAEV 1116

Query: 782  ------------RKLFGLRARSPPLPYLLSGLLQSRTHPKLSSDQTGENGDSDIDLADLS 841
                        RK+FGLR RSPPLPYLLSGLLQSRTHPKL+SDQ+G+NGDSDIDLAD+S
Sbjct: 1117 GNLSKAPETFDHRKIFGLRGRSPPLPYLLSGLLQSRTHPKLASDQSGDNGDSDIDLADMS 1176

Query: 842  DSDQEEEEDEYDQLPPFKPLRKSQIAKLSKEQRKAYFEEYDYRVKLLQKKQWKEELKRMR 901
            DSDQEEEEDEYDQLPPFKPLRKSQI+KLSKEQRKAYFEEYDYRVKLLQKKQWKEELKRMR
Sbjct: 1177 DSDQEEEEDEYDQLPPFKPLRKSQISKLSKEQRKAYFEEYDYRVKLLQKKQWKEELKRMR 1236

Query: 902  DIKKKGQPTVDDYGYMGEDDQENGTPAAVQVPLPDMALPPSFDGDNPAYRFRFLEPTSQF 961
            DIKKKGQPTV+DYGYMGEDDQEN +PAAVQVPLPDMALPPSFDGDNPAYRFRFLEPTSQF
Sbjct: 1237 DIKKKGQPTVNDYGYMGEDDQENSSPAAVQVPLPDMALPPSFDGDNPAYRFRFLEPTSQF 1296

Query: 962  LARPVLDTHGWDHDCGYDGVNLEHSMAIVSRFPAAVAVQITKDKKEFSIHLDSSVSAKHG 1011
            LARPVLDTHGWDHDCGYDGVNLEHSMAIV+RFPAAVAVQITKDKKEF+IHLDSSVSAKHG
Sbjct: 1297 LARPVLDTHGWDHDCGYDGVNLEHSMAIVNRFPAAVAVQITKDKKEFNIHLDSSVSAKHG 1356

BLAST of Lag0030990 vs. ExPASy TrEMBL
Match: A0A6J1F515 (translocase of chloroplast 159, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111442225 PE=3 SV=1)

HSP 1 Score: 1506.1 bits (3898), Expect = 0.0e+00
Identity = 849/1160 (73.19%), Postives = 903/1160 (77.84%), Query Frame = 0

Query: 1    MEKGSLVAEKQADGEVDLNDKVAAEDGEQLTKLETSSPVEDKAVLGDD--------ENSK 60
            M+KGS VAE QAD ++ LN+   AEDGE+L KLE+ S V+ KA   D         ENSK
Sbjct: 530  MDKGSSVAEMQADRKIILNEMEDAEDGERLPKLESGSSVDSKADQDDQANSKVAELENSK 589

Query: 61   NLEPADGEQEAEMDKGSPVAEKQEEEEIKLNDIVDAEDGERLTKLETGSSFDNKADQDDQ 120
             LE ADG +EAE+DKGSPVAE Q + EI LND  DAEDGE+  KLE GSS D+KADQDDQ
Sbjct: 590  FLELADGGKEAEVDKGSPVAEIQADGEIILNDKEDAEDGEQFPKLEPGSSVDSKADQDDQ 649

Query: 121  ANSKVSDLADESTGAVLDDKILHDSSPVSGTDAVGNPEEIKDVENREIADLVHGAAKLDN 180
            ANSKV++LADE T +VLD+KILH+SS VS T AVGNP EIKDV NRE ADLVHGAAKLDN
Sbjct: 650  ANSKVAELADEFTDSVLDNKILHESSLVSVTAAVGNPVEIKDVGNRETADLVHGAAKLDN 709

Query: 181  GFDNDGHEADESVEHNSVVSSTEIDNKVSEVSIAVATEEAAAHVDRAIAAADIAKNENLE 240
            GFDN GHE D+SV+HNS+VS+ EIDN + EVSIAVA EE  +H DR I+A+DIAKNENL 
Sbjct: 710  GFDNVGHETDDSVDHNSLVSNPEIDNNMLEVSIAVAAEETVSHGDREISASDIAKNENLA 769

Query: 241  VTDVEDQQPAELGASTVNEEREIVSLVDSPAEAGNEKDSKDNFEAREVVDGEVESEPSQE 300
              DVEDQQP                         +EKDSK + + RE + GEVE +PSQE
Sbjct: 770  AMDVEDQQP-------------------------DEKDSKYDSKIREDLPGEVEPKPSQE 829

Query: 301  ATSLVKENIPDNASLEDSRISDAPKIHEPVLNEVDGGKHPLDEEGDIEGSITDGETEGEI 360
            A SLVKE+IPDNAS+ DS ISDAPK  +PVLNEVDG KHPLDEEGDIEGS+TDGETEGEI
Sbjct: 830  ARSLVKESIPDNASVIDSGISDAPKALKPVLNEVDGEKHPLDEEGDIEGSVTDGETEGEI 889

Query: 361  FGSSEAAREFLQELERASGAGSHSGADSSIDHSQRIDGQIVTDSDEADTDDEGDGKQLFD 420
            FGSSEAAREF++ELERASGAG HSGA+SSID SQRIDGQIVTDSDEADTDDEGDGK+LFD
Sbjct: 890  FGSSEAAREFMEELERASGAGFHSGAESSIDQSQRIDGQIVTDSDEADTDDEGDGKELFD 949

Query: 421  SAALAALLKAARDAGSDGGPITVTSQDGSRLFSIERPAGLGSSLTSGKNASRPSRPLSFA 480
            SAALAALLKAARDAGSDGGPITVTSQDGSRLFSIERPAGLGSSL +GKNASRPSRPLSFA
Sbjct: 950  SAALAALLKAARDAGSDGGPITVTSQDGSRLFSIERPAGLGSSLVTGKNASRPSRPLSFA 1009

Query: 481  PSNPR--------------------------------RLGVSPDDSLVAQVLYRFGLVAG 540
            P+NPR                                RLGVSPDDSLVAQVLYRFGLVAG
Sbjct: 1010 PANPRVGDDSENKLSEEEKSKLQKLQQKRVNFLRLVQRLGVSPDDSLVAQVLYRFGLVAG 1069

Query: 541  RSS---------------------------------GKSGVGKSATINSIFGEDKTPINA 600
            RS+                                 GKSGVGKSATINSIFGEDKTPINA
Sbjct: 1070 RSTGQLFSFDNAKNTAMQLEAEGKEDLDFSLNILVLGKSGVGKSATINSIFGEDKTPINA 1129

Query: 601  FGPGTTTVKEIVGTVEGVKIRVFDTPGLRSSSSERGINHKILFSIKNVMKKFPPDIVLYV 660
            FGPGTT+VKEI+GTVEGVKIR+FD+PGLRSSSSER INH+IL SIKNVMKKFPPDIVLYV
Sbjct: 1130 FGPGTTSVKEIIGTVEGVKIRIFDSPGLRSSSSERRINHRILSSIKNVMKKFPPDIVLYV 1189

Query: 661  DRLDNQTRDLNDLLLLRSVSSSLGSSIWKNAIITLTHAASAPPDGPSGSPLGYEVFVAQR 720
            DRLDNQTRDLNDLLLLRSVSS LGSS+WKNAIITLTHAASAPPDGPSGSPLGYEVFVAQR
Sbjct: 1190 DRLDNQTRDLNDLLLLRSVSSYLGSSVWKNAIITLTHAASAPPDGPSGSPLGYEVFVAQR 1249

Query: 721  SHVLQQTVAQAVGDLRMLNPTLMNPVSLVENHPSCRKNRDGQ------------------ 780
            SHVLQQTVAQAVGDLR+LNPTLMNPVSLVENHPSCRKNRDGQ                  
Sbjct: 1250 SHVLQQTVAQAVGDLRILNPTLMNPVSLVENHPSCRKNRDGQKVLPNGQSWRPQLLLLCF 1309

Query: 781  --------------------RKLFGLRARSPPLPYLLSGLLQSRTHPKLSSDQTGENGDS 840
                                RKLFGLR+RSPPLPYLLSGLLQSRTHPKL SDQ G+NGDS
Sbjct: 1310 SIKILAEVGNLSKAPETFDHRKLFGLRSRSPPLPYLLSGLLQSRTHPKLPSDQNGDNGDS 1369

Query: 841  DIDLADLSDSDQEEEEDEYDQLPPFKPLRKSQIAKLSKEQRKAYFEEYDYRVKLLQKKQW 900
            DIDLADLSDSDQEEEEDEYDQLPPFKPLR SQ++KLSKEQRKAYFEEYDYRVKLLQKKQW
Sbjct: 1370 DIDLADLSDSDQEEEEDEYDQLPPFKPLRNSQVSKLSKEQRKAYFEEYDYRVKLLQKKQW 1429

Query: 901  KEELKRMRDIKKKGQPTVDDYGYMGEDDQENGTPAAVQVPLPDMALPPSFDGDNPAYRFR 960
            KEELKRMRD+KK  QPTVDDYGYMGEDDQENG PAAVQVPLPDMALPPSFDGDNPAYRFR
Sbjct: 1430 KEELKRMRDMKKGQQPTVDDYGYMGEDDQENGGPAAVQVPLPDMALPPSFDGDNPAYRFR 1489

Query: 961  FLEPTSQFLARPVLDTHGWDHDCGYDGVNLEHSMAIVSRFPAAVAVQITKDKKEFSIHLD 1006
            FLEPTSQFLARPVLDTHGWDHDCGYDGVNLEHSMAIVSRFPAAVAVQITKDKKEF+IHLD
Sbjct: 1490 FLEPTSQFLARPVLDTHGWDHDCGYDGVNLEHSMAIVSRFPAAVAVQITKDKKEFNIHLD 1549

BLAST of Lag0030990 vs. ExPASy TrEMBL
Match: A0A6J1J989 (translocase of chloroplast 159, chloroplastic-like isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111482463 PE=3 SV=1)

HSP 1 Score: 1486.1 bits (3846), Expect = 0.0e+00
Identity = 841/1160 (72.50%), Postives = 898/1160 (77.41%), Query Frame = 0

Query: 1    MEKGSLVAEKQADGEVDLNDKVAAEDGEQLTKLETSSPVEDKAVLGDD--------ENSK 60
            M+KGS VA  QAD +  LN+   AEDGEQL KLE+ S V+ KA   D         ENSK
Sbjct: 135  MDKGSSVAMMQADRKTILNEMEDAEDGEQLPKLESGSSVDSKADQDDQANSKVAELENSK 194

Query: 61   NLEPADGEQEAEMDKGSPVAEKQEEEEIKLNDIVDAEDGERLTKLETGSSFDNKADQDDQ 120
             LE ADG +EAE+DKGSPVAE Q + EI LND  DAEDGE+  KLE GSS D+KADQDDQ
Sbjct: 195  FLEVADGGKEAEVDKGSPVAEIQADGEI-LNDKEDAEDGEQFPKLEPGSSVDSKADQDDQ 254

Query: 121  ANSKVSDLADESTGAVLDDKILHDSSPVSGTDAVGNPEEIKDVENREIADLVHGAAKLDN 180
            ANSKV++LADE T +VLD+KILH+SS VS T AVGNPEEIKDV NRE ADL +GAAKLDN
Sbjct: 255  ANSKVAELADEFTDSVLDNKILHESSLVSVTAAVGNPEEIKDVGNRETADLENGAAKLDN 314

Query: 181  GFDNDGHEADESVEHNSVVSSTEIDNKVSEVSIAVATEEAAAHVDRAIAAADIAKNENLE 240
            GFDN GHE D+SV+ NS+VS+ EIDN + EVSIAVA EEA +H DR I A+DIAKNENL 
Sbjct: 315  GFDNVGHETDDSVDLNSLVSNPEIDNNMLEVSIAVAAEEAVSHGDREIVASDIAKNENLA 374

Query: 241  VTDVEDQQPAELGASTVNEEREIVSLVDSPAEAGNEKDSKDNFEAREVVDGEVESEPSQE 300
              DVEDQQP                         +E+DSK + + RE + G+VE +PSQE
Sbjct: 375  AMDVEDQQP-------------------------DEQDSKYDSKIREDLPGKVEPKPSQE 434

Query: 301  ATSLVKENIPDNASLEDSRISDAPKIHEPVLNEVDGGKHPLDEEGDIEGSITDGETEGEI 360
            A SLVKE+IPDNAS+ DS ISD P+  +PVLNEVDG KHPLDEEGDIEGS+TDGETEGEI
Sbjct: 435  ARSLVKESIPDNASVIDSGISDVPEALKPVLNEVDGAKHPLDEEGDIEGSVTDGETEGEI 494

Query: 361  FGSSEAAREFLQELERASGAGSHSGADSSIDHSQRIDGQIVTDSDEADTDDEGDGKQLFD 420
            FGSSEAAREF++ELERASGAG HSGA+SSID SQRIDGQIVTDSDEADTDDEGDGK+LFD
Sbjct: 495  FGSSEAAREFMEELERASGAGFHSGAESSIDQSQRIDGQIVTDSDEADTDDEGDGKELFD 554

Query: 421  SAALAALLKAARDAGSDGGPITVTSQDGSRLFSIERPAGLGSSLTSGKNASRPSRPLSFA 480
            SAALAALLKAARDAGSDGGPITVTSQDGSRLFSIERPAGLGSSL +GKNASRPSRPLSF 
Sbjct: 555  SAALAALLKAARDAGSDGGPITVTSQDGSRLFSIERPAGLGSSLVTGKNASRPSRPLSFT 614

Query: 481  PSNPR--------------------------------RLGVSPDDSLVAQVLYRFGLVAG 540
            P+NPR                                RLGVSPDDSLVAQVLYRFGLVAG
Sbjct: 615  PANPRVGDDSENKLSEEEKSKLQKLQQKRVNFLRLVQRLGVSPDDSLVAQVLYRFGLVAG 674

Query: 541  RSS---------------------------------GKSGVGKSATINSIFGEDKTPINA 600
            RS+                                 GKSGVGKSATINSIFGEDKTPINA
Sbjct: 675  RSTGQLFSFDNAKNTAMQLEAEGKEDLDFSLNILVLGKSGVGKSATINSIFGEDKTPINA 734

Query: 601  FGPGTTTVKEIVGTVEGVKIRVFDTPGLRSSSSERGINHKILFSIKNVMKKFPPDIVLYV 660
            FGPGTT+VKEI+GTVEGVKIR+FD+PGLRSSSSER INH+IL SIKNVMKKFPPDIVLYV
Sbjct: 735  FGPGTTSVKEIIGTVEGVKIRIFDSPGLRSSSSERRINHRILSSIKNVMKKFPPDIVLYV 794

Query: 661  DRLDNQTRDLNDLLLLRSVSSSLGSSIWKNAIITLTHAASAPPDGPSGSPLGYEVFVAQR 720
            DRLDNQTRDLNDLLLLRSVSS LGSS+WKNAIITLTHAASAPPDGPSGSPLGYEVFVAQR
Sbjct: 795  DRLDNQTRDLNDLLLLRSVSSYLGSSVWKNAIITLTHAASAPPDGPSGSPLGYEVFVAQR 854

Query: 721  SHVLQQTVAQAVGDLRMLNPTLMNPVSLVENHPSCRKNRDGQ------------------ 780
            SHVLQQTVAQAVGDLR+LNPTLMNPVSLVENHPSCRKNRDGQ                  
Sbjct: 855  SHVLQQTVAQAVGDLRILNPTLMNPVSLVENHPSCRKNRDGQKVLPNGQSWRPQLLLLCF 914

Query: 781  --------------------RKLFGLRARSPPLPYLLSGLLQSRTHPKLSSDQTGENGDS 840
                                RKLFGLR+RSPPLPYLLSGLLQSRTHPKL SDQ G+NGDS
Sbjct: 915  SIKVLAEVGNLSKAPETFDHRKLFGLRSRSPPLPYLLSGLLQSRTHPKLPSDQNGDNGDS 974

Query: 841  DIDLADLSDSDQEEEEDEYDQLPPFKPLRKSQIAKLSKEQRKAYFEEYDYRVKLLQKKQW 900
            DIDLA+L DSDQEEEEDEYDQLPPFKPLRKSQ++KLSKEQRKAYFEEYDYRVKLLQKKQW
Sbjct: 975  DIDLAELYDSDQEEEEDEYDQLPPFKPLRKSQVSKLSKEQRKAYFEEYDYRVKLLQKKQW 1034

Query: 901  KEELKRMRDIKKKGQPTVDDYGYMGEDDQENGTPAAVQVPLPDMALPPSFDGDNPAYRFR 960
            KEELKRMRD+KK  QPTVDDYGYMGEDDQENG PAAVQVPLPDMALPPSFDGDNPAYRFR
Sbjct: 1035 KEELKRMRDMKKGQQPTVDDYGYMGEDDQENGGPAAVQVPLPDMALPPSFDGDNPAYRFR 1094

Query: 961  FLEPTSQFLARPVLDTHGWDHDCGYDGVNLEHSMAIVSRFPAAVAVQITKDKKEFSIHLD 1006
            FLEPTSQFLARPVLDTHGWDHDCGYDGVNLEHSMAIVSRFPAAVAVQITKDKKEF+IHLD
Sbjct: 1095 FLEPTSQFLARPVLDTHGWDHDCGYDGVNLEHSMAIVSRFPAAVAVQITKDKKEFNIHLD 1154

BLAST of Lag0030990 vs. ExPASy TrEMBL
Match: A0A6J1J9C9 (translocase of chloroplast 159, chloroplastic-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111482463 PE=3 SV=1)

HSP 1 Score: 1486.1 bits (3846), Expect = 0.0e+00
Identity = 841/1160 (72.50%), Postives = 898/1160 (77.41%), Query Frame = 0

Query: 1    MEKGSLVAEKQADGEVDLNDKVAAEDGEQLTKLETSSPVEDKAVLGDD--------ENSK 60
            M+KGS VA  QAD +  LN+   AEDGEQL KLE+ S V+ KA   D         ENSK
Sbjct: 464  MDKGSSVAMMQADRKTILNEMEDAEDGEQLPKLESGSSVDSKADQDDQANSKVAELENSK 523

Query: 61   NLEPADGEQEAEMDKGSPVAEKQEEEEIKLNDIVDAEDGERLTKLETGSSFDNKADQDDQ 120
             LE ADG +EAE+DKGSPVAE Q + EI LND  DAEDGE+  KLE GSS D+KADQDDQ
Sbjct: 524  FLEVADGGKEAEVDKGSPVAEIQADGEI-LNDKEDAEDGEQFPKLEPGSSVDSKADQDDQ 583

Query: 121  ANSKVSDLADESTGAVLDDKILHDSSPVSGTDAVGNPEEIKDVENREIADLVHGAAKLDN 180
            ANSKV++LADE T +VLD+KILH+SS VS T AVGNPEEIKDV NRE ADL +GAAKLDN
Sbjct: 584  ANSKVAELADEFTDSVLDNKILHESSLVSVTAAVGNPEEIKDVGNRETADLENGAAKLDN 643

Query: 181  GFDNDGHEADESVEHNSVVSSTEIDNKVSEVSIAVATEEAAAHVDRAIAAADIAKNENLE 240
            GFDN GHE D+SV+ NS+VS+ EIDN + EVSIAVA EEA +H DR I A+DIAKNENL 
Sbjct: 644  GFDNVGHETDDSVDLNSLVSNPEIDNNMLEVSIAVAAEEAVSHGDREIVASDIAKNENLA 703

Query: 241  VTDVEDQQPAELGASTVNEEREIVSLVDSPAEAGNEKDSKDNFEAREVVDGEVESEPSQE 300
              DVEDQQP                         +E+DSK + + RE + G+VE +PSQE
Sbjct: 704  AMDVEDQQP-------------------------DEQDSKYDSKIREDLPGKVEPKPSQE 763

Query: 301  ATSLVKENIPDNASLEDSRISDAPKIHEPVLNEVDGGKHPLDEEGDIEGSITDGETEGEI 360
            A SLVKE+IPDNAS+ DS ISD P+  +PVLNEVDG KHPLDEEGDIEGS+TDGETEGEI
Sbjct: 764  ARSLVKESIPDNASVIDSGISDVPEALKPVLNEVDGAKHPLDEEGDIEGSVTDGETEGEI 823

Query: 361  FGSSEAAREFLQELERASGAGSHSGADSSIDHSQRIDGQIVTDSDEADTDDEGDGKQLFD 420
            FGSSEAAREF++ELERASGAG HSGA+SSID SQRIDGQIVTDSDEADTDDEGDGK+LFD
Sbjct: 824  FGSSEAAREFMEELERASGAGFHSGAESSIDQSQRIDGQIVTDSDEADTDDEGDGKELFD 883

Query: 421  SAALAALLKAARDAGSDGGPITVTSQDGSRLFSIERPAGLGSSLTSGKNASRPSRPLSFA 480
            SAALAALLKAARDAGSDGGPITVTSQDGSRLFSIERPAGLGSSL +GKNASRPSRPLSF 
Sbjct: 884  SAALAALLKAARDAGSDGGPITVTSQDGSRLFSIERPAGLGSSLVTGKNASRPSRPLSFT 943

Query: 481  PSNPR--------------------------------RLGVSPDDSLVAQVLYRFGLVAG 540
            P+NPR                                RLGVSPDDSLVAQVLYRFGLVAG
Sbjct: 944  PANPRVGDDSENKLSEEEKSKLQKLQQKRVNFLRLVQRLGVSPDDSLVAQVLYRFGLVAG 1003

Query: 541  RSS---------------------------------GKSGVGKSATINSIFGEDKTPINA 600
            RS+                                 GKSGVGKSATINSIFGEDKTPINA
Sbjct: 1004 RSTGQLFSFDNAKNTAMQLEAEGKEDLDFSLNILVLGKSGVGKSATINSIFGEDKTPINA 1063

Query: 601  FGPGTTTVKEIVGTVEGVKIRVFDTPGLRSSSSERGINHKILFSIKNVMKKFPPDIVLYV 660
            FGPGTT+VKEI+GTVEGVKIR+FD+PGLRSSSSER INH+IL SIKNVMKKFPPDIVLYV
Sbjct: 1064 FGPGTTSVKEIIGTVEGVKIRIFDSPGLRSSSSERRINHRILSSIKNVMKKFPPDIVLYV 1123

Query: 661  DRLDNQTRDLNDLLLLRSVSSSLGSSIWKNAIITLTHAASAPPDGPSGSPLGYEVFVAQR 720
            DRLDNQTRDLNDLLLLRSVSS LGSS+WKNAIITLTHAASAPPDGPSGSPLGYEVFVAQR
Sbjct: 1124 DRLDNQTRDLNDLLLLRSVSSYLGSSVWKNAIITLTHAASAPPDGPSGSPLGYEVFVAQR 1183

Query: 721  SHVLQQTVAQAVGDLRMLNPTLMNPVSLVENHPSCRKNRDGQ------------------ 780
            SHVLQQTVAQAVGDLR+LNPTLMNPVSLVENHPSCRKNRDGQ                  
Sbjct: 1184 SHVLQQTVAQAVGDLRILNPTLMNPVSLVENHPSCRKNRDGQKVLPNGQSWRPQLLLLCF 1243

Query: 781  --------------------RKLFGLRARSPPLPYLLSGLLQSRTHPKLSSDQTGENGDS 840
                                RKLFGLR+RSPPLPYLLSGLLQSRTHPKL SDQ G+NGDS
Sbjct: 1244 SIKVLAEVGNLSKAPETFDHRKLFGLRSRSPPLPYLLSGLLQSRTHPKLPSDQNGDNGDS 1303

Query: 841  DIDLADLSDSDQEEEEDEYDQLPPFKPLRKSQIAKLSKEQRKAYFEEYDYRVKLLQKKQW 900
            DIDLA+L DSDQEEEEDEYDQLPPFKPLRKSQ++KLSKEQRKAYFEEYDYRVKLLQKKQW
Sbjct: 1304 DIDLAELYDSDQEEEEDEYDQLPPFKPLRKSQVSKLSKEQRKAYFEEYDYRVKLLQKKQW 1363

Query: 901  KEELKRMRDIKKKGQPTVDDYGYMGEDDQENGTPAAVQVPLPDMALPPSFDGDNPAYRFR 960
            KEELKRMRD+KK  QPTVDDYGYMGEDDQENG PAAVQVPLPDMALPPSFDGDNPAYRFR
Sbjct: 1364 KEELKRMRDMKKGQQPTVDDYGYMGEDDQENGGPAAVQVPLPDMALPPSFDGDNPAYRFR 1423

Query: 961  FLEPTSQFLARPVLDTHGWDHDCGYDGVNLEHSMAIVSRFPAAVAVQITKDKKEFSIHLD 1006
            FLEPTSQFLARPVLDTHGWDHDCGYDGVNLEHSMAIVSRFPAAVAVQITKDKKEF+IHLD
Sbjct: 1424 FLEPTSQFLARPVLDTHGWDHDCGYDGVNLEHSMAIVSRFPAAVAVQITKDKKEFNIHLD 1483

BLAST of Lag0030990 vs. TAIR 10
Match: AT4G02510.1 (translocon at the outer envelope membrane of chloroplasts 159 )

HSP 1 Score: 832.4 bits (2149), Expect = 3.9e-241
Identity = 564/1217 (46.34%), Postives = 721/1217 (59.24%), Query Frame = 0

Query: 3    KGSLVAEKQADGEVDLNDKVAAEDGEQLTKLE--------TSSPVEDKAVLGDDE--NSK 62
            K  +V    A+G  +L     AE  E   K +           PV DKA+   +E   S+
Sbjct: 243  KPGVVVVGDAEGSEELKINADAETLEVANKFDQIGDDDSGEFEPVSDKAIEEVEEKFTSE 302

Query: 63   NLEPADGEQEAEMDKGSPVAEKQEEEEIKLNDIVDAEDGERLTKLETGSSF--------- 122
            +   AD  +   +D  +      E E +      + +D E+   LE G ++         
Sbjct: 303  SDSIADSSKLESVDTSA-----VEPEVVAAESGSEPKDVEKANGLEKGMTYAEVIKAASA 362

Query: 123  --DNKADQDDQANSKVSDLADESTGAVLDDK--ILHDSSPVSGTDA-VGNP--EEIKDVE 182
              DN   +++     + D A+E  G  L++K   + DSS +   +  V  P    + DVE
Sbjct: 363  VADNGTKEEESVLGGIVDDAEE--GVKLNNKGDFVVDSSAIEAVNVDVAKPGVVVVGDVE 422

Query: 183  NREIADLVHGAAKLDNGFDNDGH--------EADESVEH---------NSVVSSTEIDNK 242
              E+ +       + N FD  G         E+D++ E          +S+V S+ +D+ 
Sbjct: 423  VSEVLETDGNIPDVHNKFDPIGQGEGGEVELESDKATEEGGGKLVSEGDSMVDSSVVDSV 482

Query: 243  VSEVSIAVATEEAAAHVDRAIAAADIAKNENLEVTDVEDQQPAELGAS-------TVNEE 302
             +++++A            A+   D  K++ ++ T    ++P +L A+        V E 
Sbjct: 483  DADINVAEPGVVVVGAAKEAVIKED-DKDDEVDKTISNIEEPDDLTAAYDGNFELAVKEI 542

Query: 303  REIVSLVDSPAEAGNEKDSKDNFEAREV--VDGEVESEPSQEATSLVKENIPDNASLEDS 362
             E   +     + G E +     E+ +V  VD E +S P+ E+   V++ +  +++ ED 
Sbjct: 543  SEAAKVEPDEPKVGVEVEELPVSESLKVGSVDAEEDSIPAAESQFEVRKVVEGDSAEEDE 602

Query: 363  RISDAPKIHEPVLNEVDGGKHPLDEEGD------IEGSITDGETEGEIFGSSEAAREFLQ 422
                   I      E   G   +D+E        ++GS ++ ETE  IFGSSEAA++FL 
Sbjct: 603  NKLPVEDIVSS--REFSFGGKEVDQEPSGEGVTRVDGSESEEETEEMIFGSSEAAKQFLA 662

Query: 423  ELERA-SGAGSHSG-ADSSIDHSQRIDGQIVTDSDE-ADTDDEGDGKQLFDSAALAALLK 482
            ELE+A SG  +HS  A+ S + S RIDGQIVTDSDE  DT+DEG+ K +FD+AALAALLK
Sbjct: 663  ELEKASSGIEAHSDEANISNNMSDRIDGQIVTDSDEDVDTEDEGEEK-MFDTAALAALLK 722

Query: 483  AARDAG-SDGGPITVTSQDGSRLFSIERPAGLGSSLTSGKNASRP--SRPLSFAPSNP-- 542
            AA   G S+GG  T+TSQDG++LFS++RPAGL SSL   K A+ P  +R   F+ SN   
Sbjct: 723  AATGGGSSEGGNFTITSQDGTKLFSMDRPAGLSSSLRPLKPAAAPRANRSNIFSNSNVTM 782

Query: 543  ------------------------------RRLGVSPDDSLVAQVLYRFGLVAGRSS--- 602
                                          +RLG S +DS+ AQVLYR  L+AGR +   
Sbjct: 783  ADETEINLSEEEKQKLEKLQSLRVKFLRLLQRLGHSAEDSIAAQVLYRLALLAGRQAGQL 842

Query: 603  ------------------------------GKSGVGKSATINSIFGEDKTPINAFGPGTT 662
                                          GK+GVGKSATINSI G     I+AFG  TT
Sbjct: 843  FSLDAAKKKAVESEAEGNEELIFSLNILVLGKAGVGKSATINSILGNQIASIDAFGLSTT 902

Query: 663  TVKEIVGTVEGVKIRVFDTPGLRSSSSERGINHKILFSIKNVMKKFPPDIVLYVDRLDNQ 722
            +V+EI GTV GVKI   DTPGL+S++ ++  N K+L S+K VMKK PPDIVLYVDRLD Q
Sbjct: 903  SVREISGTVNGVKITFIDTPGLKSAAMDQSTNAKMLSSVKKVMKKCPPDIVLYVDRLDTQ 962

Query: 723  TRDLNDLLLLRSVSSSLGSSIWKNAIITLTHAASAPPDGPSGSPLGYEVFVAQRSHVLQQ 782
            TRDLN+L LLR++++SLG+SIWKNAI+TLTHAASAPPDGPSG+PL Y+VFVAQ SH++QQ
Sbjct: 963  TRDLNNLPLLRTITASLGTSIWKNAIVTLTHAASAPPDGPSGTPLSYDVFVAQCSHIVQQ 1022

Query: 783  TVAQAVGDLRMLNPTLMNPVSLVENHPSCRKNRDG------------------------- 842
            ++ QAVGDLR++NP+LMNPVSLVENHP CRKNR+G                         
Sbjct: 1023 SIGQAVGDLRLMNPSLMNPVSLVENHPLCRKNREGVKVLPNGQTWRSQLLLLCYSLKVLS 1082

Query: 843  -------------QRKLFGLRARSPPLPYLLSGLLQSRTHPKLSSDQTGENGDSDIDLAD 902
                          RK+FG R RSPPLPYLLS LLQSR HPKL  DQ G++ DSDI++ D
Sbjct: 1083 ETNSLLRPQEPLDHRKVFGFRVRSPPLPYLLSWLLQSRAHPKLPGDQGGDSVDSDIEIDD 1142

Query: 903  LSDSDQEE-EEDEYDQLPPFKPLRKSQIAKLSKEQRKAYFEEYDYRVKLLQKKQWKEELK 962
            +SDS+QE+ E+DEYDQLPPFKPLRK+Q+AKLS EQRKAYFEEYDYRVKLLQKKQW+EELK
Sbjct: 1143 VSDSEQEDGEDDEYDQLPPFKPLRKTQLAKLSNEQRKAYFEEYDYRVKLLQKKQWREELK 1202

Query: 963  RMRDIKKKGQPTVD-DYGYMG-EDDQENGTPAAVQVPLPDMALPPSFDGDNPAYRFRFLE 1006
            RM+++KK G+   + ++GY G EDD ENG PAAV VPLPDM LPPSFD DN AYR+R+LE
Sbjct: 1203 RMKEMKKNGKKLGESEFGYPGEEDDPENGAPAAVPVPLPDMVLPPSFDSDNSAYRYRYLE 1262

BLAST of Lag0030990 vs. TAIR 10
Match: AT2G16640.1 (multimeric translocon complex in the outer envelope membrane 132 )

HSP 1 Score: 489.2 bits (1258), Expect = 8.1e-138
Identity = 285/644 (44.25%), Postives = 371/644 (57.61%), Query Frame = 0

Query: 478  RLGVSPDDSLVAQVLYRFGL---VAGRSS------------------------------- 537
            RLG +P + +VAQVLYR GL   + GR+                                
Sbjct: 517  RLGQTPHNVVVAQVLYRLGLAEQLRGRNGSRVGAFSFDRASAMAEQLEAAGQDPLDFSCT 576

Query: 538  ----GKSGVGKSATINSIFGEDKTPINAFGPGTTTVKEIVGTVEGVKIRVFDTPGLRSSS 597
                GKSGVGKSATINSIF E K   +AF  GT  V+++ G V+G+K+RV DTPGL  S 
Sbjct: 577  IMVLGKSGVGKSATINSIFDEVKFCTDAFQMGTKRVQDVEGLVQGIKVRVIDTPGLLPSW 636

Query: 598  SERGINHKILFSIKNVMKKFPPDIVLYVDRLDNQTRDLNDLLLLRSVSSSLGSSIWKNAI 657
            S++  N KIL S+K  +KK PPDIVLY+DRLD Q+RD  D+ LLR++S   G SIW NAI
Sbjct: 637  SDQAKNEKILNSVKAFIKKNPPDIVLYLDRLDMQSRDSGDMPLLRTISDVFGPSIWFNAI 696

Query: 658  ITLTHAASAPPDGPSGSPLGYEVFVAQRSHVLQQTVAQAVGDLRMLNPTLMNPVSLVENH 717
            + LTHAAS PPDGP+G+   Y++FV QRSHV+QQ + QA GD+R     LMNPVSLVENH
Sbjct: 697  VGLTHAASVPPDGPNGTASSYDMFVTQRSHVIQQAIRQAAGDMR-----LMNPVSLVENH 756

Query: 718  PSCRKNRDGQRKL-------------------------------------FGLRARSPPL 777
             +CR NR GQR L                                     F  R+++PPL
Sbjct: 757  SACRTNRAGQRVLPNGQVWKPHLLLLSFASKILAEANALLKLQDNIPGRPFAARSKAPPL 816

Query: 778  PYLLSGLLQSRTHPKLSSDQTGENGDSDIDLADLSDSDQEEEEDEYDQLPPFKPLRKSQI 837
            P+LLS LLQSR  PKL   Q G+  D D    DL +S   +EE EYDQLPPFK L K+Q+
Sbjct: 817  PFLLSSLLQSRPQPKLPEQQYGDEEDED----DLEESSDSDEESEYDQLPPFKSLTKAQM 876

Query: 838  AKLSKEQRKAYFEEYDYRVKLLQKKQWKEELKRMRDIKKKGQPTVD-DYGYMGEDDQENG 897
            A LSK Q+K Y +E +YR KLL KKQ KEE KR +  KK      D   GY    ++E+G
Sbjct: 877  ATLSKSQKKQYLDEMEYREKLLMKKQMKEERKRRKMFKKFAAEIKDLPDGYSENVEEESG 936

Query: 898  TPAAVQVPLPDMALPPSFDGDNPAYRFRFLEPTSQFLARPVLDTHGWDHDCGYDGVNLEH 957
             PA+V VP+PD++LP SFD DNP +R+R+L+ ++Q+L RPVL+THGWDHD GY+GVN E 
Sbjct: 937  GPASVPVPMPDLSLPASFDSDNPTHRYRYLDSSNQWLVRPVLETHGWDHDIGYEGVNAER 996

Query: 958  SMAIVSRFPAAVAVQITKDKKEFSIHLDSSVSAKHGENGSTMAGF--------------- 1002
               +  + P +V+ Q+TKDKK+ ++ L+ + S KHGE  ST  GF               
Sbjct: 997  LFVVKEKIPISVSGQVTKDKKDANVQLEMASSVKHGEGKSTSLGFDMQTVGKELAYTLRS 1056

BLAST of Lag0030990 vs. TAIR 10
Match: AT3G16620.1 (translocon outer complex protein 120 )

HSP 1 Score: 446.8 bits (1148), Expect = 4.6e-125
Identity = 371/1096 (33.85%), Postives = 529/1096 (48.27%), Query Frame = 0

Query: 19   NDKVAAEDGEQLTKLETSSPVEDKAVLGDDENSKNLEPADGEQEAEMDKGSPVAEKQEEE 78
            ++K  AEDG ++++L  S  V+       D   +  E A G QE    +       QE+ 
Sbjct: 14   DEKKLAEDG-RISELVGSDEVK-------DNEEEVFEEAIGSQEGLKPESLKTDVLQEDF 73

Query: 79   EIKLND-IVDAEDGER---------LTKLETGSSFDNKADQDDQANSKVSDLA-----DE 138
             +  ND + D E+  R         +   E G S     +Q        SDL      D 
Sbjct: 74   PLASNDEVCDLEETSRNERGVENLKVNYSEIGESHGEVNEQCITTKEADSDLVTLKMNDY 133

Query: 139  STGAVLDDKILHDSSPVSGTDAVGNPEEIKDVENREIADLVHGAAKLDNGFDNDGHEADE 198
              G V D  I +     S  D V N E       +  ++L      L+NG   + H + E
Sbjct: 134  DHGEVADADISY-GKMASSLDVVENSE-------KATSNLATEDVNLENG---NTHSSSE 193

Query: 199  SVEHNSVVSSTEIDNKVSEV-SIAVATEEAAAH--------VDRAIAAADIAKNENLEVT 258
                N VVS  E    V+EV S++  + E  ++         +  ++A  + +  N +  
Sbjct: 194  ----NGVVSPDENKELVAEVISVSACSVETGSNGIDDEKWEEEIDVSAGMVTEQRNGKTG 253

Query: 259  DVEDQQPAELGASTVNEEREIVSLVDSPAEAGNEKDSKDNFEAREVVDGEVESEPSQEAT 318
               +      G  ++N+  E+ +   SP E  + ++            GE ES+ S    
Sbjct: 254  AEFNSVKIVSGDKSLNDSIEVAAGTLSPLEKSSSEEK-----------GETESQNSNGGH 313

Query: 319  SLVKENIPDNASLEDSRISDAPKIHEPVLNEVDGGKHPLDEEGDIEGSITDGETEGEIFG 378
             +  ++  +    +DS ++  P+I E            ++ E ++  S++  E+  +   
Sbjct: 314  DI--QSNKEIVKQQDSSVNIGPEIKE---------SQHMERESEVLSSVSPTESRSDTAA 373

Query: 379  SSEAAREFLQE----LERASGAGSHSGADSSIDHSQRIDGQIVTDSDEADTDDEGDGKQL 438
               A    L      LE A         + ++ H+Q    Q   DS  A+TD+  + ++ 
Sbjct: 374  LPPARPAGLGRAAPLLEPAPRVTQQPRVNGNVSHNQ---PQQAEDSTTAETDEHDETREK 433

Query: 439  FDSAALAALLKAARDAGSDGGPITVTSQDGSRLFSIERPAGLGSSLT---SGKNASRPSR 498
                 +  L  + R   +      V +Q   RL   E+  G   S     S   AS  + 
Sbjct: 434  LQFIRVKFLRLSHRLGQTPHN--VVVAQVLYRLGLAEQLRGRNGSRVGAFSFDRASAMAE 493

Query: 499  PLSFAPSNPRRLGVSPDDSLVAQVLYRFGLVAGRSSGKSGVGKSATINSIFGEDKTPINA 558
             L  A  +P       D S    VL           GKSGVGKSATINSIF E K   +A
Sbjct: 494  QLEAAAQDPL------DFSCTIMVL-----------GKSGVGKSATINSIFDELKISTDA 553

Query: 559  FGPGTTTVKEIVGTVEGVKIRVFDTPGLRSSSSERGINHKILFSIKNVMKKFPPDIVLYV 618
            F  GT  V++I G V+G+K+RV DTPGL  S S++  N KIL S++  +KK PPDIVLY+
Sbjct: 554  FQVGTKKVQDIEGFVQGIKVRVIDTPGLLPSWSDQHKNEKILKSVRAFIKKSPPDIVLYL 613

Query: 619  DRLDNQTRDLNDLLLLRSVSSSLGSSIWKNAIITLTHAASAPPDGPSGSPLGYEVFVAQR 678
            DRLD Q+RD  D+ LLR+++   G SIW NAI+ LTHAASAPPDGP+G+   Y++FV QR
Sbjct: 614  DRLDMQSRDSGDMPLLRTITDVFGPSIWFNAIVGLTHAASAPPDGPNGTASSYDMFVTQR 673

Query: 679  SHVLQQTVAQAVGDLRMLNPTLMNPVSLVENHPSCRKNRDGQRKL--------------- 738
            SHV+QQ + QA GD+R     LMNPVSLVENH +CR NR GQR L               
Sbjct: 674  SHVIQQAIRQAAGDMR-----LMNPVSLVENHSACRTNRAGQRVLPNGQVWKPHLLLLSF 733

Query: 739  ----------------------FGLRARSPPLPYLLSGLLQSRTHPKLSSDQTGENGDSD 798
                                  F  R+++PPLP LLS LLQSR   KL   Q  +  D D
Sbjct: 734  ASKILAEANALLKLQDNIPGGQFATRSKAPPLPLLLSSLLQSRPQAKLPEQQYDDEDDED 793

Query: 799  IDLADLSDSDQEEEEDEYDQLPPFKPLRKSQIAKLSKEQRKAYFEEYDYRVKLLQKKQWK 858
                DL +S   EEE EYD+LPPFK L K+++ KLSK Q+K Y +E +YR KL  K+Q K
Sbjct: 794  ----DLDESSDSEEESEYDELPPFKRLTKAEMTKLSKSQKKEYLDEMEYREKLFMKRQMK 853

Query: 859  EELKRMRDIKKKGQPTVD-DYGYMGEDDQENGTPAAVQVPLPDMALPPSFDGDNPAYRFR 918
            EE KR + +KK      D   GY    ++E   PA+V VP+PD++LP SFD DNP +R+R
Sbjct: 854  EERKRRKLLKKFAAEIKDMPNGYSENVEEERSEPASVPVPMPDLSLPASFDSDNPTHRYR 913

Query: 919  FLEPTSQFLARPVLDTHGWDHDCGYDGVNLEHSMAIVSRFPAAVAVQITKDKKEFSIHLD 978
            +L+ ++Q+L RPVL+THGWDHD GY+GVN E    +  + P + + Q+TKDKK+  + L+
Sbjct: 914  YLDTSNQWLVRPVLETHGWDHDIGYEGVNAERLFVVKDKIPVSFSGQVTKDKKDAHVQLE 973

Query: 979  SSVSAKHGENGSTMAGF------------------------------------------- 1002
             + S KHGE  ST  GF                                           
Sbjct: 974  LASSVKHGEGRSTSLGFDMQNAGKELAYTIRSETRFNKFRKNKAAAGLSVTLLGDSVSAG 1033

BLAST of Lag0030990 vs. TAIR 10
Match: AT5G20300.1 (Avirulence induced gene (AIG1) family protein )

HSP 1 Score: 319.3 bits (817), Expect = 1.1e-86
Identity = 215/577 (37.26%), Postives = 298/577 (51.65%), Query Frame = 0

Query: 504 GKSGVGKSATINSIFGEDKTPINAFGPGTTTVKEIVGTVEGVKIRVFDTPGLRS-SSSER 563
           GK+GVGKSATINSIFG+ K+  +AF PGT  ++E++GTV GVK+   DTPG    SSS  
Sbjct: 173 GKTGVGKSATINSIFGQPKSETDAFRPGTDRIEEVMGTVSGVKVTFIDTPGFHPLSSSST 232

Query: 564 GINHKILFSIKNVMKKFPPDIVLYVDRLDNQTRDLNDLLLLRSVSSSLGSSIWKNAIITL 623
             N KIL SIK  +KK PPD+VLY+DRLD      +D  LL+ ++   G++IW N I+ +
Sbjct: 233 RKNRKILLSIKRYVKKRPPDVVLYLDRLDMIDMRYSDFSLLQLITEIFGAAIWLNTILVM 292

Query: 624 THAASAPPDGPSGSPLGYEVFVAQRSHVLQQTVAQAVGDLRMLNPTLMNPVSLVENHPSC 683
           TH+A A  +G +G  + YE +V QR  V+Q  + QAV D +     L NPV LVENHPSC
Sbjct: 293 THSA-ATTEGRNGQSVNYESYVGQRMDVVQHYIHQAVSDTK-----LENPVLLVENHPSC 352

Query: 684 RKNRDGQ--------------------------------RKLFGL----RARSPPLPYLL 743
           +KN  G+                                R   GL      R+  LP+LL
Sbjct: 353 KKNLAGEYVLPNGVVWKPQFMFLCVCTKVLGDVQSLLRFRDSIGLGQPSSTRTASLPHLL 412

Query: 744 SGLLQSRTHPKLSSDQTGENGDSDIDLADLSDSDQEEEEDEYDQLPPFKPLRKSQIAKLS 803
           S  L+ R      +D+T +  D  ++L DL      EEEDEYDQLP  + L KS+  KLS
Sbjct: 413 SVFLRRRL--SSGADETEKEIDKLLNL-DL------EEEDEYDQLPTIRILGKSRFEKLS 472

Query: 804 KEQRKAYFEEYDYRVKLLQKKQWKEELKRMRDIKKKGQPTVDDYGYMGEDDQENGTPAAV 863
           K Q+K Y +E DYR  L  KKQ KEE +R RD K   +  +        +D E    AA 
Sbjct: 473 KSQKKEYLDELDYRETLYLKKQLKEECRRRRDEKLVEEENL--------EDTEQRDQAA- 532

Query: 864 QVPLPDMALPPSFDGDNPAYRFRFLEPTSQFLARPVLDTHGWDHDCGYDGVNLEHSMAIV 923
            VPLPDMA P SFD D PA+R+R +    Q+L RPV D  GWD D G+DG+N+E +  I 
Sbjct: 533 -VPLPDMAGPDSFDSDFPAHRYRCVSAGDQWLVRPVYDPQGWDRDVGFDGINIETAAKIN 592

Query: 924 SRFPAAVAVQITKDKKEFSIHLDSSV---------------------------------- 983
               A+   Q+++DK+ F+I  +++                                   
Sbjct: 593 RNLFASATGQVSRDKQRFTIQSETNAAYTRNFREQTFSVAVDLQSSGEDLVYSFQGGTKL 652

Query: 984 -SAKHGE----------NGSTMAGFKVEDQITLGKRVVLVGSTGTVRSQGDSAFGANLEM 999
            + KH             G    G K+ED + +GKRV L  + G +R  G +A G + E 
Sbjct: 653 QTFKHNTTDVGVGLTSFGGKYYVGGKLEDTLLVGKRVKLTANAGQMRGSGQTANGGSFEA 712

BLAST of Lag0030990 vs. TAIR 10
Match: AT5G20300.2 (Avirulence induced gene (AIG1) family protein )

HSP 1 Score: 319.3 bits (817), Expect = 1.1e-86
Identity = 215/577 (37.26%), Postives = 298/577 (51.65%), Query Frame = 0

Query: 504 GKSGVGKSATINSIFGEDKTPINAFGPGTTTVKEIVGTVEGVKIRVFDTPGLRS-SSSER 563
           GK+GVGKSATINSIFG+ K+  +AF PGT  ++E++GTV GVK+   DTPG    SSS  
Sbjct: 173 GKTGVGKSATINSIFGQPKSETDAFRPGTDRIEEVMGTVSGVKVTFIDTPGFHPLSSSST 232

Query: 564 GINHKILFSIKNVMKKFPPDIVLYVDRLDNQTRDLNDLLLLRSVSSSLGSSIWKNAIITL 623
             N KIL SIK  +KK PPD+VLY+DRLD      +D  LL+ ++   G++IW N I+ +
Sbjct: 233 RKNRKILLSIKRYVKKRPPDVVLYLDRLDMIDMRYSDFSLLQLITEIFGAAIWLNTILVM 292

Query: 624 THAASAPPDGPSGSPLGYEVFVAQRSHVLQQTVAQAVGDLRMLNPTLMNPVSLVENHPSC 683
           TH+A A  +G +G  + YE +V QR  V+Q  + QAV D +     L NPV LVENHPSC
Sbjct: 293 THSA-ATTEGRNGQSVNYESYVGQRMDVVQHYIHQAVSDTK-----LENPVLLVENHPSC 352

Query: 684 RKNRDGQ--------------------------------RKLFGL----RARSPPLPYLL 743
           +KN  G+                                R   GL      R+  LP+LL
Sbjct: 353 KKNLAGEYVLPNGVVWKPQFMFLCVCTKVLGDVQSLLRFRDSIGLGQPSSTRTASLPHLL 412

Query: 744 SGLLQSRTHPKLSSDQTGENGDSDIDLADLSDSDQEEEEDEYDQLPPFKPLRKSQIAKLS 803
           S  L+ R      +D+T +  D  ++L DL      EEEDEYDQLP  + L KS+  KLS
Sbjct: 413 SVFLRRRL--SSGADETEKEIDKLLNL-DL------EEEDEYDQLPTIRILGKSRFEKLS 472

Query: 804 KEQRKAYFEEYDYRVKLLQKKQWKEELKRMRDIKKKGQPTVDDYGYMGEDDQENGTPAAV 863
           K Q+K Y +E DYR  L  KKQ KEE +R RD K   +  +        +D E    AA 
Sbjct: 473 KSQKKEYLDELDYRETLYLKKQLKEECRRRRDEKLVEEENL--------EDTEQRDQAA- 532

Query: 864 QVPLPDMALPPSFDGDNPAYRFRFLEPTSQFLARPVLDTHGWDHDCGYDGVNLEHSMAIV 923
            VPLPDMA P SFD D PA+R+R +    Q+L RPV D  GWD D G+DG+N+E +  I 
Sbjct: 533 -VPLPDMAGPDSFDSDFPAHRYRCVSAGDQWLVRPVYDPQGWDRDVGFDGINIETAAKIN 592

Query: 924 SRFPAAVAVQITKDKKEFSIHLDSSV---------------------------------- 983
               A+   Q+++DK+ F+I  +++                                   
Sbjct: 593 RNLFASATGQVSRDKQRFTIQSETNAAYTRNFREQTFSVAVDLQSSGEDLVYSFQGGTKL 652

Query: 984 -SAKHGE----------NGSTMAGFKVEDQITLGKRVVLVGSTGTVRSQGDSAFGANLEM 999
            + KH             G    G K+ED + +GKRV L  + G +R  G +A G + E 
Sbjct: 653 QTFKHNTTDVGVGLTSFGGKYYVGGKLEDTLLVGKRVKLTANAGQMRGSGQTANGGSFEA 712