Lag0030942 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0030942
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	aspartic proteinase PCS1-like
Location	chr11: 3036475 .. 3036879 (-)
RNA-Seq Expression	Lag0030942
Synteny	Lag0030942

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGATCGACTCCGGTTCGGACCTGAGCTATCTAGTGGACGAAGCGTACGACAAGGTGAGAGAAGAGGTGGTGAGACTGGTGGGGCCGATGATGAAGAAGGGGTACGAATACGCCGCCGTGGCCGACATGTGTTTCGACGGCGGAGCGGCGGCGGAGGTGGGGCGGAGGATCGGCGACATGTCGTTCGAGTTCGAGAACGGGGTGGAGATTTTGGTGGGGAAAGGGGAAGGGGTATTGACGGAAGTGGAAAAAGGAGTGAAGTGCGTGGGGATCGGACGGTCAGGAAGGCTCGGGATTGGGAGTAATATAATCGGGAACGTTCATCAGCAGAATATGTGGGTCGAGTACGATTTGGCCAATAGGAGAATCGGGTTTGGTGGAGCTGACTGTAGTGGATTGAAATGA

mRNA sequence

ATGATCGACTCCGGTTCGGACCTGAGCTATCTAGTGGACGAAGCGTACGACAAGGTGAGAGAAGAGGTGGTGAGACTGGTGGGGCCGATGATGAAGAAGGGGTACGAATACGCCGCCGTGGCCGACATGTGTTTCGACGGCGGAGCGGCGGCGGAGGTGGGGCGGAGGATCGGCGACATGTCGTTCGAGTTCGAGAACGGGGTGGAGATTTTGGTGGGGAAAGGGGAAGGGGTATTGACGGAAGTGGAAAAAGGAGTGAAGTGCGTGGGGATCGGACGGTCAGGAAGGCTCGGGATTGGGAGTAATATAATCGGGAACGTTCATCAGCAGAATATGTGGGTCGAGTACGATTTGGCCAATAGGAGAATCGGGTTTGGTGGAGCTGACTGTAGTGGATTGAAATGA

Coding sequence (CDS)

ATGATCGACTCCGGTTCGGACCTGAGCTATCTAGTGGACGAAGCGTACGACAAGGTGAGAGAAGAGGTGGTGAGACTGGTGGGGCCGATGATGAAGAAGGGGTACGAATACGCCGCCGTGGCCGACATGTGTTTCGACGGCGGAGCGGCGGCGGAGGTGGGGCGGAGGATCGGCGACATGTCGTTCGAGTTCGAGAACGGGGTGGAGATTTTGGTGGGGAAAGGGGAAGGGGTATTGACGGAAGTGGAAAAAGGAGTGAAGTGCGTGGGGATCGGACGGTCAGGAAGGCTCGGGATTGGGAGTAATATAATCGGGAACGTTCATCAGCAGAATATGTGGGTCGAGTACGATTTGGCCAATAGGAGAATCGGGTTTGGTGGAGCTGACTGTAGTGGATTGAAATGA

Protein sequence

MIDSGSDLSYLVDEAYDKVREEVVRLVGPMMKKGYEYAAVADMCFDGGAAAEVGRRIGDMSFEFENGVEILVGKGEGVLTEVEKGVKCVGIGRSGRLGIGSNIIGNVHQQNMWVEYDLANRRIGFGGADCSGLK

Homology

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BLAST of Lag0030942 vs. NCBI nr
Match: XP_011651212.2 (aspartic proteinase PCS1 [Cucumis sativus] >KAE8650483.1 hypothetical protein Csa_010014 [Cucumis sativus])

HSP 1 Score: 244.2 bits (622), Expect = 6.4e-61
Identity = 117/134 (87.31%), Postives = 125/134 (93.28%), Query Frame = 0


Query: 1   MIDSGSDLSYLVDEAYDKVREEVVRLVGPMMKKGYEYAAVADMCFDGGAAAEVGRRIGDM 60
           MIDSGSDL+YLVDEAY+KV+EEVVRLVG MMKKGY YAAVADMCFD G   EVGRRIGDM
Sbjct: 298 MIDSGSDLTYLVDEAYEKVKEEVVRLVGAMMKKGYVYAAVADMCFDAGVTVEVGRRIGDM 357

Query: 61  SFEFENGVEILVGKGEGVLTEVEKGVKCVGIGRSGRLGIGSNIIGNVHQQNMWVEYDLAN 120
           SFEF+NGVEI VG+GEGVLTEVEKGVKCVGIGRSGRLGIGSNIIG VHQQNMWVEYDLAN
Sbjct: 358 SFEFDNGVEIFVGRGEGVLTEVEKGVKCVGIGRSGRLGIGSNIIGTVHQQNMWVEYDLAN 417

Query: 121 RRIGFGGADCSGLK 135
           +R+GFGGA+CS LK
Sbjct: 418 KRVGFGGAECSRLK 431

[Close]

BLAST of Lag0030942 vs. NCBI nr
Match: XP_038878004.1 (aspartic proteinase PCS1 [Benincasa hispida])

HSP 1 Score: 242.7 bits (618), Expect = 1.9e-60
Identity = 117/134 (87.31%), Postives = 126/134 (94.03%), Query Frame = 0


Query: 1   MIDSGSDLSYLVDEAYDKVREEVVRLVGPMMKKGYEYAAVADMCFDGGAAAEVGRRIGDM 60
           MIDSGSDL+YL DEAY+KV+EE+VRLVGPMMKKGYE+AAVADMCF+ G AA VGRRIG+M
Sbjct: 291 MIDSGSDLTYLADEAYEKVKEEIVRLVGPMMKKGYEFAAVADMCFNAGEAAVVGRRIGNM 350

Query: 61  SFEFENGVEILVGKGEGVLTEVEKGVKCVGIGRSGRLGIGSNIIGNVHQQNMWVEYDLAN 120
           SFEFENGVEILVGKGEGVL EVEKGVKCVGIGRSGRLGIGSNIIG VHQQNMWVEYDLA 
Sbjct: 351 SFEFENGVEILVGKGEGVLAEVEKGVKCVGIGRSGRLGIGSNIIGTVHQQNMWVEYDLAK 410

Query: 121 RRIGFGGADCSGLK 135
           +RIGFGGA+CS LK
Sbjct: 411 KRIGFGGAECSRLK 424

[Close]

BLAST of Lag0030942 vs. NCBI nr
Match: XP_022983616.1 (aspartic proteinase PCS1-like [Cucurbita maxima])

HSP 1 Score: 240.0 bits (611), Expect = 1.2e-59
Identity = 116/134 (86.57%), Postives = 124/134 (92.54%), Query Frame = 0


Query: 1   MIDSGSDLSYLVDEAYDKVREEVVRLVGPMMKKGYEYAAVADMCFDGGAAAEVGRRIGDM 60
           MIDSGSDL+YLVDEAY+KV+EE+V+LVGP+MKKGYEYAAVADMCF+ G  AEVGRRI DM
Sbjct: 286 MIDSGSDLTYLVDEAYEKVKEEIVKLVGPLMKKGYEYAAVADMCFNDGETAEVGRRIRDM 345

Query: 61  SFEFENGVEILVGKGEGVLTEVEKGVKCVGIGRSGRLGIGSNIIGNVHQQNMWVEYDLAN 120
           SFEFENGVEI VGKGEGVLTEVEKGVKCVG GRSGRLGI SNIIG VHQ+N WVEYDLAN
Sbjct: 346 SFEFENGVEISVGKGEGVLTEVEKGVKCVGFGRSGRLGIASNIIGTVHQKNTWVEYDLAN 405

Query: 121 RRIGFGGADCSGLK 135
           RRIGFGGADCS LK
Sbjct: 406 RRIGFGGADCSRLK 419

[Close]

BLAST of Lag0030942 vs. NCBI nr
Match: KAG6581233.1 (Aspartic proteinase PCS1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 240.0 bits (611), Expect = 1.2e-59
Identity = 116/134 (86.57%), Postives = 124/134 (92.54%), Query Frame = 0


Query: 1   MIDSGSDLSYLVDEAYDKVREEVVRLVGPMMKKGYEYAAVADMCFDGGAAAEVGRRIGDM 60
           MIDSGSDL+YLVDEAY+KV+EE+V+LVGP+MKKGYEYAAVADMCF+ G  AEVGRRI DM
Sbjct: 290 MIDSGSDLTYLVDEAYEKVKEEIVKLVGPLMKKGYEYAAVADMCFNDGETAEVGRRIRDM 349

Query: 61  SFEFENGVEILVGKGEGVLTEVEKGVKCVGIGRSGRLGIGSNIIGNVHQQNMWVEYDLAN 120
           SFEFENGVEI VGKGEGVLTEVEKGVKCVG GRSGRLGI SNIIG VHQ+N WVEYDLAN
Sbjct: 350 SFEFENGVEISVGKGEGVLTEVEKGVKCVGFGRSGRLGIASNIIGTVHQKNTWVEYDLAN 409

Query: 121 RRIGFGGADCSGLK 135
           RRIGFGGADCS LK
Sbjct: 410 RRIGFGGADCSRLK 423

[Close]

BLAST of Lag0030942 vs. NCBI nr
Match: XP_022934878.1 (aspartic proteinase PCS1-like [Cucurbita moschata])

HSP 1 Score: 239.6 bits (610), Expect = 1.6e-59
Identity = 115/134 (85.82%), Postives = 124/134 (92.54%), Query Frame = 0


Query: 1   MIDSGSDLSYLVDEAYDKVREEVVRLVGPMMKKGYEYAAVADMCFDGGAAAEVGRRIGDM 60
           MIDSGSDL+YLVDEAY+KV+EE+V+LVGP+MKKGYEYAAVADMCF+ G  AEVGRRI DM
Sbjct: 290 MIDSGSDLTYLVDEAYEKVKEEIVKLVGPLMKKGYEYAAVADMCFNDGETAEVGRRIRDM 349

Query: 61  SFEFENGVEILVGKGEGVLTEVEKGVKCVGIGRSGRLGIGSNIIGNVHQQNMWVEYDLAN 120
           SFEFENGVEI VGKGEGVLTEVEKGVKCVG GRSGRLGI SNIIG VHQ+N W+EYDLAN
Sbjct: 350 SFEFENGVEISVGKGEGVLTEVEKGVKCVGFGRSGRLGIASNIIGTVHQKNTWIEYDLAN 409

Query: 121 RRIGFGGADCSGLK 135
           RRIGFGGADCS LK
Sbjct: 410 RRIGFGGADCSRLK 423

[Close]

BLAST of Lag0030942 vs. ExPASy Swiss-Prot
Match: Q9LZL3 (Aspartic proteinase PCS1 OS=Arabidopsis thaliana OX=3702 GN=PCS1 PE=2 SV=1)

HSP 1 Score: 60.5 bits (145), Expect = 1.7e-08
Identity = 40/143 (27.97%), Postives = 67/143 (46.85%), Query Frame = 0


Query: 1   MIDSGSDLSYLVDEAYDKVREEVVRLVGPMM----KKGYEYAAVADMCFDGGAA---AEV 60
           M+DSG+  ++L+   Y  +R   +     ++       + +    D+C+        + +
Sbjct: 302 MVDSGTQFTFLLGPVYTALRSHFLNRTNGILTVYEDPDFVFQGTMDLCYRISPVRIRSGI 361

Query: 61  GRRIGDMSFEFENGVEILVGKGEGVLTEV------EKGVKCVGIGRSGRLGIGSNIIGNV 120
             R+  +S  FE G EI V  G+ +L  V         V C   G S  +G+ + +IG+ 
Sbjct: 362 LHRLPTVSLVFE-GAEIAV-SGQPLLYRVPHLTVGNDSVYCFTFGNSDLMGMEAYVIGHH 421

Query: 121 HQQNMWVEYDLANRRIGFGGADC 131
           HQQNMW+E+DL   RIG    +C
Sbjct: 422 HQQNMWIEFDLQRSRIGLAPVEC 442

[Close]

BLAST of Lag0030942 vs. ExPASy Swiss-Prot
Match: Q766C3 (Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 SV=1)

HSP 1 Score: 58.9 bits (141), Expect = 5.0e-08
Identity = 40/130 (30.77%), Postives = 61/130 (46.92%), Query Frame = 0


Query: 1   MIDSGSDLSYLVDEAYDKVREEVVRLVGPMMKKGYEYAAVADMCFDGGAAAEVGRRIGDM 60
           +IDSG+ L+Y V+ AY  VR+E +  +   +  G   ++  D+CF   +      +I   
Sbjct: 313 IIDSGTTLTYFVNNAYQSVRQEFISQINLPVVNG--SSSGFDLCFQTPSDPS-NLQIPTF 372

Query: 61  SFEFENGVEILVGKGEGVLTEVEKGVKCVGIGRSGRLGIGSNIIGNVHQQNMWVEYDLAN 120
              F+ G   L    E        G+ C+ +G S +   G +I GN+ QQNM V YD  N
Sbjct: 373 VMHFDGG--DLELPSENYFISPSNGLICLAMGSSSQ---GMSIFGNIQQQNMLVVYDTGN 432

Query: 121 RRIGFGGADC 131
             + F  A C
Sbjct: 433 SVVSFASAQC 434

[Close]

BLAST of Lag0030942 vs. ExPASy Swiss-Prot
Match: Q9LS40 (Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana OX=3702 GN=ASPG1 PE=1 SV=1)

HSP 1 Score: 51.2 bits (121), Expect = 1.0e-05
Identity = 33/130 (25.38%), Postives = 63/130 (48.46%), Query Frame = 0


Query: 1   MIDSGSDLSYLVDEAYDKVREEVVRLVGPMMKKGYEYAAVADMCFDGGAAAEVGRRIGDM 60
           ++D G+ ++ L  +AY+ +R+  ++L    +KKG    ++ D C+D  + + V  ++  +
Sbjct: 377 ILDCGTAVTRLQTQAYNSLRDAFLKLT-VNLKKGSSSISLFDTCYDFSSLSTV--KVPTV 436

Query: 61  SFEFENGVEILVGKGEGVLTEVEKGVKCVGIGRSGRLGIGSNIIGNVHQQNMWVEYDLAN 120
           +F F  G  + +     ++   + G  C     +       +IIGNV QQ   + YDL+ 
Sbjct: 437 AFHFTGGKSLDLPAKNYLIPVDDSGTFCFAFAPTSS---SLSIIGNVQQQGTRITYDLSK 496

Query: 121 RRIGFGGADC 131
             IG  G  C
Sbjct: 497 NVIGLSGNKC 500

[Close]

BLAST of Lag0030942 vs. ExPASy Swiss-Prot
Match: Q9LTW4 (Aspartic proteinase NANA, chloroplast OS=Arabidopsis thaliana OX=3702 GN=NANA PE=1 SV=1)

HSP 1 Score: 51.2 bits (121), Expect = 1.0e-05
Identity = 35/131 (26.72%), Postives = 64/131 (48.85%), Query Frame = 0


Query: 1   MIDSGSDLSYLVDEAYDKVREEVVRLVGPMMKKGYEYAAVADMCFDGGAAAEVGRRIGDM 60
           ++DSG+ L+ L D AY +V   + R +  + +   E   + + CF   +   V  ++  +
Sbjct: 336 ILDSGTSLTLLADAAYKQVVTGLARYLVELKRVKPEGVPI-EYCFSFTSGFNVS-KLPQL 395

Query: 61  SFEFENGVEILVGKGEGVLTEVEKGVKCVGIGRSGRLGIGSNIIGNVHQQNMWVEYDLAN 120
           +F  + G      + +  L +   GVKC+G   +G     +N+IGN+ QQN   E+DL  
Sbjct: 396 TFHLKGGARFEPHR-KSYLVDAAPGVKCLGFVSAGT--PATNVIGNIMQQNYLWEFDLMA 455

Query: 121 RRIGFGGADCS 132
             + F  + C+
Sbjct: 456 STLSFAPSACT 461

[Close]

BLAST of Lag0030942 vs. ExPASy Swiss-Prot
Match: Q6F4N5 (Aspartyl protease 25 OS=Oryza sativa subsp. japonica OX=39947 GN=AP25 PE=2 SV=1)

HSP 1 Score: 48.9 bits (115), Expect = 5.2e-05
Identity = 33/132 (25.00%), Postives = 61/132 (46.21%), Query Frame = 0


Query: 1   MIDSGSDLSYLVDEAYDKVREEVVRLVGPMMKKGYEYAAVADMCFDGGAAAEVGRRIGDM 60
           ++DSG+ ++      Y  +REE  R V      GY      D CF+    A  G     +
Sbjct: 311 VVDSGTVITRWTAPVYAALREEFRRQVA--APSGYTSLGAFDTCFNTDEVAAGGAPA--V 370

Query: 61  SFEFENGVEILVGKGEGVLTEVEKGVKCVGIGRSGR-LGIGSNIIGNVHQQNMWVEYDLA 120
           +   + GV++ +     ++      + C+ +  + + +    N+I N+ QQN+ V +D+A
Sbjct: 371 TVHMDGGVDLALPMENTLIHSSATPLACLAMAEAPQNVNSVVNVIANLQQQNIRVVFDVA 430

Query: 121 NRRIGFGGADCS 132
           N R+GF    C+
Sbjct: 431 NSRVGFAKESCN 438

The following BLAST results are available for this feature:

BLAST of Lag0030942 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_011651212.2	6.4e-61	87.31	aspartic proteinase PCS1 [Cucumis sativus] >KAE8650483.1 hypothetical protein Cs...	[more]
XP_038878004.1	1.9e-60	87.31	aspartic proteinase PCS1 [Benincasa hispida]	[more]
XP_022983616.1	1.2e-59	86.57	aspartic proteinase PCS1-like [Cucurbita maxima]	[more]
KAG6581233.1	1.2e-59	86.57	Aspartic proteinase PCS1, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022934878.1	1.6e-59	85.82	aspartic proteinase PCS1-like [Cucurbita moschata]	[more]

BLAST of Lag0030942 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LZL3	1.7e-08	27.97	Aspartic proteinase PCS1 OS=Arabidopsis thaliana OX=3702 GN=PCS1 PE=2 SV=1	[more]
Q766C3	5.0e-08	30.77	Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 S...	[more]
Q9LS40	1.0e-05	25.38	Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana OX=3702 GN=ASP...	[more]
Q9LTW4	1.0e-05	26.72	Aspartic proteinase NANA, chloroplast OS=Arabidopsis thaliana OX=3702 GN=NANA PE...	[more]
Q6F4N5	5.2e-05	25.00	Aspartyl protease 25 OS=Oryza sativa subsp. japonica OX=39947 GN=AP25 PE=2 SV=1	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR032799	Xylanase inhibitor, C-terminal	PFAM	PF14541	TAXi_C	coord: 1..125 e-value: 1.8E-22 score: 79.9
IPR021109	Aspartic peptidase domain superfamily	GENE3D	2.40.70.10	Acid Proteases	coord: 1..133 e-value: 3.5E-27 score: 97.2
IPR021109	Aspartic peptidase domain superfamily	SUPERFAMILY	50630	Acid proteases	coord: 1..130
IPR001461	Aspartic peptidase A1 family	PANTHER	PTHR47965	ASPARTYL PROTEASE-RELATED	coord: 1..131
None	No IPR available	PANTHER	PTHR47965:SF51	EUKARYOTIC ASPARTYL PROTEASE FAMILY PROTEIN	coord: 1..131
IPR033121	Peptidase family A1 domain	PROSITE	PS51767	PEPTIDASE_A1	coord: 1..126 score: 17.228497

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0030942.1	Lag0030942.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
molecular_function	GO:0004190	aspartic-type endopeptidase activity