Lag0030881 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0030881 vs. NCBI nr
Match: XP_004151203.3 (major latex protein 149 [Cucumis sativus] >KAE8650619.1 hypothetical protein Csa_011700 [Cucumis sativus])

HSP 1 Score: 197.6 bits (501), Expect = 7.7e-47
Identity = 97/152 (63.82%), Postives = 118/152 (77.63%), Query Frame = 0

Query: 1   MAQIAKISEQVQLKCSAQRFYECFKN-MDCLPRLFPENIKSYKIVEGNGFAHGSVVHWKY 60
           MAQIAKISEQVQLKCS  +FY+ F N MD + ++FP  + SYKI+EGNGFAHGSV+H KY
Sbjct: 15  MAQIAKISEQVQLKCSGHKFYDFFTNKMDSVIQMFPHIVTSYKILEGNGFAHGSVIHLKY 74

Query: 61  EIDGIPTEVKERLAMDDANKSISIKFLEGDVFRDFEEFKVIIQAIDNNESNGSSVTWFVE 120
            I G P E+KERLA DDANKSI+ +  EGD+FRDFE FK+ +Q I+   SNGSSV W +E
Sbjct: 75  NIGG-PAEIKERLAFDDANKSIAFEVFEGDLFRDFEVFKMKMQVINEKGSNGSSVNWSIE 134

Query: 121 FVKANEDVAPPYNYLQLGAKISKGIDAYLCNN 152
           FVK NEDVA P++YL + A+ SK +D YLCNN
Sbjct: 135 FVKENEDVAAPHHYLTIAAQSSKTLDDYLCNN 165

BLAST of Lag0030881 vs. NCBI nr
Match: KAG6581204.1 (hypothetical protein SDJN03_21206, partial [Cucurbita argyrosperma subsp. sororia] >KAG7017943.1 hypothetical protein SDJN02_19809, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 195.7 bits (496), Expect = 2.9e-46
Identity = 94/152 (61.84%), Postives = 122/152 (80.26%), Query Frame = 0

Query: 1   MAQIAKISEQVQLKCSAQRFYECFKN-MDCLPRLFPENIKSYKIVEGNGFAHGSVVHWKY 60
           MAQIAK+S++VQL+ S  +FYE  KN MD + ++FP+  KS+K++EGNG AHGS++H KY
Sbjct: 1   MAQIAKVSQKVQLRSSGHKFYELLKNKMDFVFQMFPDVYKSWKVLEGNGLAHGSIIHLKY 60

Query: 61  EIDGIPTEVKERLAMDDANKSISIKFLEGDVFRDFEEFKVIIQAIDNNESNGSSVTWFVE 120
            +DG P+E KERLA+DDANKSI+ + LEGD+FRDFE FK+ I+ ++N  SNG S  W +E
Sbjct: 61  NVDG-PSEAKERLAIDDANKSITFECLEGDLFRDFEVFKLKIEVVENG-SNGCSTNWSIE 120

Query: 121 FVKANEDVAPPYNYLQLGAKISKGIDAYLCNN 152
           +VKANEDVAPP+NYL + AKISKGID YLC N
Sbjct: 121 YVKANEDVAPPHNYLIIAAKISKGIDDYLCKN 150

BLAST of Lag0030881 vs. NCBI nr
Match: XP_022935319.1 (kirola-like [Cucurbita moschata])

HSP 1 Score: 194.1 bits (492), Expect = 8.6e-46
Identity = 93/152 (61.18%), Postives = 122/152 (80.26%), Query Frame = 0

Query: 1   MAQIAKISEQVQLKCSAQRFYECFKN-MDCLPRLFPENIKSYKIVEGNGFAHGSVVHWKY 60
           MAQIAK+S++VQL+ S  +FYE  KN MD + ++FP+  KS+K++EGNG AHGS++H KY
Sbjct: 1   MAQIAKVSQKVQLRSSGHKFYELLKNKMDFVFQMFPDVYKSWKVLEGNGLAHGSIIHLKY 60

Query: 61  EIDGIPTEVKERLAMDDANKSISIKFLEGDVFRDFEEFKVIIQAIDNNESNGSSVTWFVE 120
            +DG P++ KERLA+DDANKSI+ + LEGD+FRDFE FK+ I+ ++N  SNG S  W +E
Sbjct: 61  NVDG-PSKAKERLAIDDANKSITFECLEGDLFRDFEVFKLKIEVVENG-SNGCSTNWSIE 120

Query: 121 FVKANEDVAPPYNYLQLGAKISKGIDAYLCNN 152
           +VKANEDVAPP+NYL + AKISKGID YLC N
Sbjct: 121 YVKANEDVAPPHNYLIIAAKISKGIDDYLCKN 150

BLAST of Lag0030881 vs. NCBI nr
Match: XP_022948007.1 (MLP-like protein 34 isoform X1 [Cucurbita moschata])

HSP 1 Score: 193.4 bits (490), Expect = 1.5e-45
Identity = 93/152 (61.18%), Postives = 118/152 (77.63%), Query Frame = 0

Query: 1   MAQIAKISEQVQLKCSAQRFYECFKN-MDCLPRLFPENIKSYKIVEGNGFAHGSVVHWKY 60
           MAQI+ IS+Q+QLKCS +RFYE FKN MD + ++FP+ + SYKIVEGNGFAHGSVVHWKY
Sbjct: 1   MAQISNISKQLQLKCSGERFYELFKNKMDSITQMFPKKLLSYKIVEGNGFAHGSVVHWKY 60

Query: 61  EIDGIPTEVKERLAMDDANKSISIKFLEGDVFRDFEEFKVIIQAIDNNESNGSSVTWFVE 120
           E+ G   E K++L MDD NK+I+++F+EGD+F+DFE   V  +  D   +  SSV W VE
Sbjct: 61  ELRG-TLEAKQKLHMDDTNKAITLEFIEGDLFKDFEMVAVKGEVSDGGSNGISSVKWSVE 120

Query: 121 FVKANEDVAPPYNYLQLGAKISKGIDAYLCNN 152
           FVKANE VAPP+NYLQ   + +KGI+AYLCNN
Sbjct: 121 FVKANEGVAPPHNYLQFALESTKGIEAYLCNN 151

BLAST of Lag0030881 vs. NCBI nr
Match: KAG6581206.1 (Major latex protein 146, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 193.0 bits (489), Expect = 1.9e-45
Identity = 95/152 (62.50%), Postives = 122/152 (80.26%), Query Frame = 0

Query: 1   MAQIAKISEQVQLKCSAQRFYECFKN-MDCLPRLFPENIKSYKIVEGNGFAHGSVVHWKY 60
           MAQIAK+S++VQL+ SA +FYE  KN MD + ++FPE  KS+K+VEGNG+AHGSV+H KY
Sbjct: 1   MAQIAKVSQEVQLRSSAPKFYEFLKNKMDFVFQMFPEIYKSWKVVEGNGYAHGSVIHLKY 60

Query: 61  EIDGIPTEVKERLAMDDANKSISIKFLEGDVFRDFEEFKVIIQAIDNNESNGSSVTWFVE 120
            +DG P+EVKERL++DDAN+SI+ + LEGD+ RDFE  K+ I+ ++N  SNGSS  W +E
Sbjct: 61  NVDG-PSEVKERLSIDDANQSITFECLEGDLLRDFEVMKMKIEVVENG-SNGSSANWSIE 120

Query: 121 FVKANEDVAPPYNYLQLGAKISKGIDAYLCNN 152
           FVKANEDVA P+NYL   AK+SKGID YLC N
Sbjct: 121 FVKANEDVATPHNYLLCVAKVSKGIDEYLCKN 150

BLAST of Lag0030881 vs. ExPASy Swiss-Prot
Match: P19825 (Major latex protein 15 OS=Papaver somniferum OX=3469 GN=MLP15 PE=2 SV=2)

HSP 1 Score: 99.8 bits (247), Expect = 2.9e-20
Identity = 45/146 (30.82%), Postives = 80/146 (54.79%), Query Frame = 0

Query: 4   IAKISEQVQLKCSAQRFYECFKNMDCLPRLFPENIKSYKIVEGNGFAHGSVVHWKYEIDG 63
           + K+  + ++ C+A ++Y+ FK+ + LP   P    S K VEG+G   G V  W Y ++G
Sbjct: 11  VGKLITESEVNCNADKYYQIFKHHEDLPSAIPHIYTSVKAVEGHGTTSGCVKEWCYILEG 70

Query: 64  IPTEVKERLAMDDANKSISIKFLEGDVFRDFEEFKVIIQAIDNNESNGSSVTWFVEFVKA 123
            P  VKE+   +D  ++I+   +EG +  D+++F   +         GS VTW V++ K 
Sbjct: 71  KPLTVKEKTTYNDETRTINHNGIEGGMMNDYKKFVATLVVKPKANGQGSIVTWIVDYEKI 130

Query: 124 NEDVAPPYNYLQLGAKISKGIDAYLC 150
           NED   P++YL    +  + ++++LC
Sbjct: 131 NEDSPVPFDYLAFFQQNIEDLNSHLC 156

BLAST of Lag0030881 vs. ExPASy Swiss-Prot
Match: Q06394 (Major latex protein 146 OS=Papaver somniferum OX=3469 GN=MLP146 PE=2 SV=1)

HSP 1 Score: 96.3 bits (238), Expect = 3.2e-19
Identity = 41/146 (28.08%), Postives = 79/146 (54.11%), Query Frame = 0

Query: 4   IAKISEQVQLKCSAQRFYECFKNMDCLPRLFPENIKSYKIVEGNGFAHGSVVHWKYEIDG 63
           + K+  + ++ C+A ++Y+ +K+ + LP + P    S K VEG+G   G V  W Y ++G
Sbjct: 11  VGKLVMESEVNCNADKYYKLYKHHEDLPSVIPHIYTSVKAVEGHGTTSGCVKEWGYILEG 70

Query: 64  IPTEVKERLAMDDANKSISIKFLEGDVFRDFEEFKVIIQAIDNNESNGSSVTWFVEFVKA 123
            P   KE+   +D  ++I    + GD+  D+++F   +     +  +G  V W +++ K 
Sbjct: 71  KPLSCKEKTTYNDETRTIHHMVVAGDLMNDYKKFDATLVVNPKSNGHGCIVKWTIDYEKM 130

Query: 124 NEDVAPPYNYLQLGAKISKGIDAYLC 150
           NED   P+ YL    +I++ + ++LC
Sbjct: 131 NEDSPVPFGYLACYQQITEDLSSHLC 156

BLAST of Lag0030881 vs. ExPASy Swiss-Prot
Match: Q06395 (Major latex protein 149 OS=Papaver somniferum OX=3469 GN=MLP149 PE=2 SV=1)

HSP 1 Score: 93.6 bits (231), Expect = 2.1e-18
Identity = 42/146 (28.77%), Postives = 78/146 (53.42%), Query Frame = 0

Query: 4   IAKISEQVQLKCSAQRFYECFKNMDCLPRLFPENIKSYKIVEGNGFAHGSVVHWKYEIDG 63
           + K+  + ++ C+A ++Y+ +K+ + LP   P  + S K VEG+G   G V  W Y  +G
Sbjct: 11  VGKLVMETEVNCNADKYYQIYKHHEDLPSAIPHIVTSAKAVEGHGTTSGCVKEWGYMHEG 70

Query: 64  IPTEVKERLAMDDANKSISIKFLEGDVFRDFEEFKVIIQAIDNNESNGSSVTWFVEFVKA 123
                KE+   +D  ++I     EGD+  D+++F   +     +  +GS V + +++ K 
Sbjct: 71  KTLTCKEKTTYNDETRTICHSISEGDLMNDYKKFDATLVVDPKDNGHGSIVKYILDYEKI 130

Query: 124 NEDVAPPYNYLQLGAKISKGIDAYLC 150
           NED   P +YL L  + ++ ++ YLC
Sbjct: 131 NEDSPVPIHYLALCNQATEDLNTYLC 156

BLAST of Lag0030881 vs. ExPASy Swiss-Prot
Match: Q9SSK7 (MLP-like protein 34 OS=Arabidopsis thaliana OX=3702 GN=MLP34 PE=2 SV=1)

HSP 1 Score: 92.8 bits (229), Expect = 3.5e-18
Identity = 49/144 (34.03%), Postives = 80/144 (55.56%), Query Frame = 0

Query: 7   ISEQVQLKCSAQRFYECFKNM-DCLPRLFPENIKSYKIVEGNGFAHGSVVHWKYEIDGIP 66
           +  +V++K SA++F+  F      + +  P NI+S  + EG+    GS+V W Y  DG  
Sbjct: 169 LETEVEIKASAEKFHHMFAGKPHHVSKATPGNIQSCDLHEGDWGTVGSIVFWNYVHDGEA 228

Query: 67  TEVKERL-AMDDANKSISIKFLEGDVFRDFEEFKVIIQAIDNNESNGSSVTWFVEFVKAN 126
              KER+ A+D     I+ + +EGD+ ++++ F + IQ    +  +GS V W  E+ K N
Sbjct: 229 KVAKERIEAVDPEKNLITFRVIEGDLMKEYKSFVITIQVTPKHGGSGSVVHWHFEYEKIN 288

Query: 127 EDVAPPYNYLQLGAKISKGIDAYL 149
           E+VA P   LQ   ++SK ID +L
Sbjct: 289 EEVAHPETLLQFAVEVSKEIDEHL 312

BLAST of Lag0030881 vs. ExPASy Swiss-Prot
Match: A0A2U9GGW3 (Thebaine synthase 2 OS=Papaver somniferum OX=3469 GN=THS2 PE=1 SV=1)

HSP 1 Score: 92.4 bits (228), Expect = 4.6e-18
Identity = 42/145 (28.97%), Postives = 77/145 (53.10%), Query Frame = 0

Query: 4   IAKISEQVQLKCSAQRFYECFKNMDCLPRLFPENIKSYKIVEGNGFAHGSVVHWKYEIDG 63
           + K+S ++++ C A+++Y  +K+ + + +  P      KI+ G+  + G +  W   IDG
Sbjct: 10  VGKLSTELEVDCDAEKYYNMYKHGEDVKKAVPHLCVDVKIISGDPTSSGCIKEWNVNIDG 69

Query: 64  IPTEVKERLAMDDANKSISIKFLEGDVFRDFEEFKVIIQAIDNNESNGSSVTWFVEFVKA 123
                 E    DD  K++  +  EGDV +DF++F  I+      + NG  VT  +E+ K 
Sbjct: 70  KTIRSVEETTHDDETKTLRHRVFEGDVMKDFKKFDTIMVVNPKPDGNGCVVTRSIEYEKT 129

Query: 124 NEDVAPPYNYLQLGAKISKGIDAYL 149
           NE+   P++YLQ G +  + ++ YL
Sbjct: 130 NENSPTPFDYLQFGHQAIEDMNKYL 154