Lag0030831 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0030831
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionDisease resistance protein
Locationchr11: 1851842 .. 1852716 (+)
RNA-Seq ExpressionLag0030831
SyntenyLag0030831
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCTCTATTGAGTCCCTCTAGGGCTGATGTGGCAGTTTCAAATAATAAAATATTGATTTTTAATTAAAATAATAATATAATAATAATAAAATAATAAAAAATTATTAAAATATAAAAAATTATTATTATTATTTAAAAAAATTATTTCTCTTCCATTTCCCCACCGTCCCTCTCCTCTTCCGCCGCCCCCACCTTGTCTCTCTTTTTCAATTACATGTGCACCAATAAAATTTTAAGATAAATGATCTACAAGAAGATGAAGCATGGAGTTTATTTAAGAAATTCACTGGTGAAATTATTGAAACATCTGATTGGAAACCTTTTGCTGTTGAGATAATAGCTAAATGTGCACGTTTGCCTGTTGTTATCACTACGGTTGCTAAAGCATTAAGAAATAGACCTTCATACATTTGGAAAGACGCTTTAGATCAACTCAGAAAACCTGTGGTTGTGAATATTAAAGGAATGAAGGCTAACGTGTATTCCCCACTAAAATTAAGTTATGAGCGACTAGAAGATGAAGAGGCCAAGTTGTTATTTTTGCTTTGTAGCATGTTTCCAGAAGATCATTCCATTGATGTGGAAGACTTGCATGTATACGCAATGTGTCTGGATTTTCTACAAGATATTAGCACTATGGCACAAGGACGGCGTAGGATCAGAAAGTTAGTTGACAATCTTATATCTTCTTCTATGCTTCTATCGTCTAAGTACAACAACCATGTAAAAATGCATGATATAATTCGTGACGTAGCCATATCAATTGCATCTACGCATGATCATATTCGTACGTTATGTTACTTGAAGAAAGAATTGAATAATAATGAAGGGAAGAAGAGAAAATATTTGGTAATCAAACTGCAGTGTACTTAA

mRNA sequence

ATGTCTCTATTGAGTCCCTCTAGGGCTGATGTGGCAATAAATGATCTACAAGAAGATGAAGCATGGAGTTTATTTAAGAAATTCACTGGTGAAATTATTGAAACATCTGATTGGAAACCTTTTGCTGTTGAGATAATAGCTAAATGTGCACGTTTGCCTGTTGTTATCACTACGGTTGCTAAAGCATTAAGAAATAGACCTTCATACATTTGGAAAGACGCTTTAGATCAACTCAGAAAACCTGTGGTTGTGAATATTAAAGGAATGAAGGCTAACGTGTATTCCCCACTAAAATTAAGTTATGAGCGACTAGAAGATGAAGAGGCCAAGTTGTTATTTTTGCTTTGTAGCATGTTTCCAGAAGATCATTCCATTGATGTGGAAGACTTGCATGTATACGCAATGTGTCTGGATTTTCTACAAGATATTAGCACTATGGCACAAGGACGGCGTAGGATCAGAAAGTTAGTTGACAATCTTATATCTTCTTCTATGCTTCTATCGTCTAAGTACAACAACCATGTAAAAATGCATGATATAATTCGTGACGTAGCCATATCAATTGCATCTACGCATGATCATATTCGTACGTTATGTTACTTGAAGAAAGAATTGAATAATAATGAAGGGAAGAAGAGAAAATATTTGGTAATCAAACTGCAGTGTACTTAA

Coding sequence (CDS)

ATGTCTCTATTGAGTCCCTCTAGGGCTGATGTGGCAATAAATGATCTACAAGAAGATGAAGCATGGAGTTTATTTAAGAAATTCACTGGTGAAATTATTGAAACATCTGATTGGAAACCTTTTGCTGTTGAGATAATAGCTAAATGTGCACGTTTGCCTGTTGTTATCACTACGGTTGCTAAAGCATTAAGAAATAGACCTTCATACATTTGGAAAGACGCTTTAGATCAACTCAGAAAACCTGTGGTTGTGAATATTAAAGGAATGAAGGCTAACGTGTATTCCCCACTAAAATTAAGTTATGAGCGACTAGAAGATGAAGAGGCCAAGTTGTTATTTTTGCTTTGTAGCATGTTTCCAGAAGATCATTCCATTGATGTGGAAGACTTGCATGTATACGCAATGTGTCTGGATTTTCTACAAGATATTAGCACTATGGCACAAGGACGGCGTAGGATCAGAAAGTTAGTTGACAATCTTATATCTTCTTCTATGCTTCTATCGTCTAAGTACAACAACCATGTAAAAATGCATGATATAATTCGTGACGTAGCCATATCAATTGCATCTACGCATGATCATATTCGTACGTTATGTTACTTGAAGAAAGAATTGAATAATAATGAAGGGAAGAAGAGAAAATATTTGGTAATCAAACTGCAGTGTACTTAA

Protein sequence

MSLLSPSRADVAINDLQEDEAWSLFKKFTGEIIETSDWKPFAVEIIAKCARLPVVITTVAKALRNRPSYIWKDALDQLRKPVVVNIKGMKANVYSPLKLSYERLEDEEAKLLFLLCSMFPEDHSIDVEDLHVYAMCLDFLQDISTMAQGRRRIRKLVDNLISSSMLLSSKYNNHVKMHDIIRDVAISIASTHDHIRTLCYLKKELNNNEGKKRKYLVIKLQCT
Homology
BLAST of Lag0030831 vs. NCBI nr
Match: XP_038890457.1 (probable disease resistance protein At4g27220 isoform X2 [Benincasa hispida])

HSP 1 Score: 244.6 bits (623), Expect = 8.2e-61
Identity = 129/202 (63.86%), Postives = 154/202 (76.24%), Query Frame = 0

Query: 13  INDLQEDEAWSLFKKFTGEIIETSDWKPFAVEIIAKCARLPVVITTVAKALRNRPSYIWK 72
           I  LQEDE W+LFKK  GEI+E  D K  A+EI  +CA LP+ I T+AKALRN+ S IWK
Sbjct: 301 IKVLQEDETWNLFKKIAGEIVEIFDLKSIAIEIAKECAHLPIAIITIAKALRNKHSSIWK 360

Query: 73  DALDQLRKPVVVNIKGMKANVYSPLKLSYERLEDEEAKLLFLLCSMFPEDHSIDVEDLHV 132
           DALDQLR P+VVNI+GM   VYS LKLSYE+L+ EE+KLLFLLCSMFPED  IDVE+LHV
Sbjct: 361 DALDQLRNPIVVNIRGMNEKVYSSLKLSYEQLDCEESKLLFLLCSMFPEDDEIDVEELHV 420

Query: 133 YAMCLDFLQDISTMAQGRRRIRKLVDNLISSSMLLSSKYNN--HVKMHDIIRDVAISIAS 192
           YAM + FL  + T+AQ RRRI KLVD+LISSS+LL    N   +VKMHD+IRDVAI I+S
Sbjct: 421 YAMGMGFLHGVDTVAQARRRITKLVDDLISSSLLLDVSKNKGVYVKMHDLIRDVAILISS 480

Query: 193 THDHIRTLCYLKKELNNNEGKK 213
            +DHIRTL +  K LN +  +K
Sbjct: 481 KNDHIRTLNF-SKGLNESWSEK 501

BLAST of Lag0030831 vs. NCBI nr
Match: XP_038890456.1 (probable disease resistance protein At4g27220 isoform X1 [Benincasa hispida])

HSP 1 Score: 244.6 bits (623), Expect = 8.2e-61
Identity = 129/202 (63.86%), Postives = 154/202 (76.24%), Query Frame = 0

Query: 13  INDLQEDEAWSLFKKFTGEIIETSDWKPFAVEIIAKCARLPVVITTVAKALRNRPSYIWK 72
           I  LQEDE W+LFKK  GEI+E  D K  A+EI  +CA LP+ I T+AKALRN+ S IWK
Sbjct: 301 IKVLQEDETWNLFKKIAGEIVEIFDLKSIAIEIAKECAHLPIAIITIAKALRNKHSSIWK 360

Query: 73  DALDQLRKPVVVNIKGMKANVYSPLKLSYERLEDEEAKLLFLLCSMFPEDHSIDVEDLHV 132
           DALDQLR P+VVNI+GM   VYS LKLSYE+L+ EE+KLLFLLCSMFPED  IDVE+LHV
Sbjct: 361 DALDQLRNPIVVNIRGMNEKVYSSLKLSYEQLDCEESKLLFLLCSMFPEDDEIDVEELHV 420

Query: 133 YAMCLDFLQDISTMAQGRRRIRKLVDNLISSSMLLSSKYNN--HVKMHDIIRDVAISIAS 192
           YAM + FL  + T+AQ RRRI KLVD+LISSS+LL    N   +VKMHD+IRDVAI I+S
Sbjct: 421 YAMGMGFLHGVDTVAQARRRITKLVDDLISSSLLLDVSKNKGVYVKMHDLIRDVAILISS 480

Query: 193 THDHIRTLCYLKKELNNNEGKK 213
            +DHIRTL +  K LN +  +K
Sbjct: 481 KNDHIRTLNF-SKGLNESWSEK 501

BLAST of Lag0030831 vs. NCBI nr
Match: XP_038890450.1 (disease resistance protein At4g27190-like isoform X1 [Benincasa hispida] >XP_038890451.1 disease resistance protein At4g27190-like isoform X1 [Benincasa hispida] >XP_038890452.1 disease resistance protein At4g27190-like isoform X1 [Benincasa hispida] >XP_038890453.1 disease resistance protein At4g27190-like isoform X1 [Benincasa hispida])

HSP 1 Score: 232.3 bits (591), Expect = 4.2e-57
Identity = 125/194 (64.43%), Postives = 145/194 (74.74%), Query Frame = 0

Query: 13  INDLQEDEAWSLFKKFTGEIIETSDWKPFAVEIIAKCARLPVVITTVAKALRNRPSYIWK 72
           I  LQEDE W+LFKK  GEI+ETS  K  AVEI  +CA LP+ I T+AKALRN+ S IWK
Sbjct: 301 IGVLQEDETWNLFKKMAGEIVETSYLKSIAVEIARECAHLPIAIITIAKALRNKHSSIWK 360

Query: 73  DALDQLRKPVVVNIKGMKANVYSPLKLSYERLEDEEAKLLFLLCSMFPEDHSIDVEDLHV 132
           DAL+QLR PVVVNIKGM   VYS LKLSY++L+ EEAKLLFLLCSMFPED  IDV+ LHV
Sbjct: 361 DALNQLRNPVVVNIKGMNEKVYSSLKLSYDQLDCEEAKLLFLLCSMFPEDAVIDVKKLHV 420

Query: 133 YAMCLDFLQDISTMAQGRRRIRKLVDNLISSSMLLSSKY----NNHVKMHDIIRDVAISI 192
           YA+ L FL  + TMAQ + RI KLVD+LISSS+L+        + +VKMHD+IRDVAI I
Sbjct: 421 YAIGLGFLHGVDTMAQAQHRIAKLVDDLISSSLLIEDSNDGWGHRYVKMHDLIRDVAILI 480

Query: 193 ASTHDHIRTLCYLK 203
           AS  D I TL Y K
Sbjct: 481 ASKDDRICTLSYSK 494

BLAST of Lag0030831 vs. NCBI nr
Match: XP_038890455.1 (disease resistance protein At4g27190-like isoform X3 [Benincasa hispida])

HSP 1 Score: 232.3 bits (591), Expect = 4.2e-57
Identity = 125/194 (64.43%), Postives = 145/194 (74.74%), Query Frame = 0

Query: 13  INDLQEDEAWSLFKKFTGEIIETSDWKPFAVEIIAKCARLPVVITTVAKALRNRPSYIWK 72
           I  LQEDE W+LFKK  GEI+ETS  K  AVEI  +CA LP+ I T+AKALRN+ S IWK
Sbjct: 301 IGVLQEDETWNLFKKMAGEIVETSYLKSIAVEIARECAHLPIAIITIAKALRNKHSSIWK 360

Query: 73  DALDQLRKPVVVNIKGMKANVYSPLKLSYERLEDEEAKLLFLLCSMFPEDHSIDVEDLHV 132
           DAL+QLR PVVVNIKGM   VYS LKLSY++L+ EEAKLLFLLCSMFPED  IDV+ LHV
Sbjct: 361 DALNQLRNPVVVNIKGMNEKVYSSLKLSYDQLDCEEAKLLFLLCSMFPEDAVIDVKKLHV 420

Query: 133 YAMCLDFLQDISTMAQGRRRIRKLVDNLISSSMLLSSKY----NNHVKMHDIIRDVAISI 192
           YA+ L FL  + TMAQ + RI KLVD+LISSS+L+        + +VKMHD+IRDVAI I
Sbjct: 421 YAIGLGFLHGVDTMAQAQHRIAKLVDDLISSSLLIEDSNDGWGHRYVKMHDLIRDVAILI 480

Query: 193 ASTHDHIRTLCYLK 203
           AS  D I TL Y K
Sbjct: 481 ASKDDRICTLSYSK 494

BLAST of Lag0030831 vs. NCBI nr
Match: XP_038890454.1 (disease resistance protein At4g27190-like isoform X2 [Benincasa hispida])

HSP 1 Score: 232.3 bits (591), Expect = 4.2e-57
Identity = 125/194 (64.43%), Postives = 145/194 (74.74%), Query Frame = 0

Query: 13  INDLQEDEAWSLFKKFTGEIIETSDWKPFAVEIIAKCARLPVVITTVAKALRNRPSYIWK 72
           I  LQEDE W+LFKK  GEI+ETS  K  AVEI  +CA LP+ I T+AKALRN+ S IWK
Sbjct: 301 IGVLQEDETWNLFKKMAGEIVETSYLKSIAVEIARECAHLPIAIITIAKALRNKHSSIWK 360

Query: 73  DALDQLRKPVVVNIKGMKANVYSPLKLSYERLEDEEAKLLFLLCSMFPEDHSIDVEDLHV 132
           DAL+QLR PVVVNIKGM   VYS LKLSY++L+ EEAKLLFLLCSMFPED  IDV+ LHV
Sbjct: 361 DALNQLRNPVVVNIKGMNEKVYSSLKLSYDQLDCEEAKLLFLLCSMFPEDAVIDVKKLHV 420

Query: 133 YAMCLDFLQDISTMAQGRRRIRKLVDNLISSSMLLSSKY----NNHVKMHDIIRDVAISI 192
           YA+ L FL  + TMAQ + RI KLVD+LISSS+L+        + +VKMHD+IRDVAI I
Sbjct: 421 YAIGLGFLHGVDTMAQAQHRIAKLVDDLISSSLLIEDSNDGWGHRYVKMHDLIRDVAILI 480

Query: 193 ASTHDHIRTLCYLK 203
           AS  D I TL Y K
Sbjct: 481 ASKDDRICTLSYSK 494

BLAST of Lag0030831 vs. ExPASy Swiss-Prot
Match: Q9T048 (Disease resistance protein At4g27190 OS=Arabidopsis thaliana OX=3702 GN=At4g27190 PE=2 SV=1)

HSP 1 Score: 115.5 bits (288), Expect = 7.5e-25
Identity = 65/183 (35.52%), Postives = 106/183 (57.92%), Query Frame = 0

Query: 10  DVAINDLQEDEAWSLFKKFTGEIIETSDWKPFAVEIIAKCARLPVVITTVAKALRNRPSY 69
           DV ++ L E++AW LF K  G+++ +   +  A  +  +C  LP+ I TV  A+R + + 
Sbjct: 296 DVRVDCLLEEDAWELFCKNAGDVVRSDHVRKIAKAVSQECGGLPLAIITVGTAMRGKKNV 355

Query: 70  -IWKDALDQLRKPVVVNIKGMKANVYSPLKLSYERLEDEEAKLLFLLCSMFPEDHSIDVE 129
            +W   L +L K V   IK ++  ++ PLKLSY+ LED +AK  FLLC++FPED+SI+V 
Sbjct: 356 KLWNHVLSKLSKSVPW-IKSIEEKIFQPLKLSYDFLED-KAKFCFLLCALFPEDYSIEVT 415

Query: 130 DLHVYAMCLDFLQDISTMAQGRRRIRKLVDNLISSSMLLSSKYNNHVKMHDIIRDVAISI 189
           ++  Y M   F++++ +           V++L    +L      + VKMHD++RD AI I
Sbjct: 416 EVVRYWMAEGFMEELGSQEDSMNEGITTVESLKDYCLLEDGDRRDTVKMHDVVRDFAIWI 475

Query: 190 AST 192
            S+
Sbjct: 476 MSS 476

BLAST of Lag0030831 vs. ExPASy Swiss-Prot
Match: Q9LMP6 (Probable disease resistance protein At1g15890 OS=Arabidopsis thaliana OX=3702 GN=At1g15890 PE=3 SV=2)

HSP 1 Score: 103.2 bits (256), Expect = 3.9e-21
Identity = 63/209 (30.14%), Postives = 107/209 (51.20%), Query Frame = 0

Query: 10  DVAINDLQEDEAWSLFKKFTGEIIETS--DWKPFAVEIIAKCARLPVVITTVAKALRNRP 69
           ++ ++ L  DEAW LF+K  G I   S  D    A ++  KC  LP+ ++ + KA+ +R 
Sbjct: 304 EMKVDCLPPDEAWELFQKKVGPIPLQSHEDIPTLARKVAEKCCGLPLALSVIGKAMASRE 363

Query: 70  S-YIWKDALDQLRKPVVVNIKGMKANVYSPLKLSYERLEDEEAKLLFLLCSMFPEDHSID 129
           +   W+  +  L          M+  +   LK SY+ L+DE+ KL FL CS+FPED+ + 
Sbjct: 364 TVQEWQHVIHVLNSS-SHEFPSMEEKILPVLKFSYDDLKDEKVKLCFLYCSLFPEDYEVR 423

Query: 130 VEDLHVYAMCLDFLQDISTMAQGRRRIRKLVDNLISSSMLLSSKYNNHVKMHDIIRDVAI 189
            E+L  Y MC  F+           +   ++ +L+ + +L+  +    VKMHD+IR++A+
Sbjct: 424 KEELIEYWMCEGFIDGNEDEDGANNKGHDIIGSLVRAHLLMDGELTTKVKMHDVIREMAL 483

Query: 190 SIASTHDHIR---------TLCYLKKELN 207
            IAS     +          LC++ K++N
Sbjct: 484 WIASNFGKQKETLCVKPGVQLCHIPKDIN 511

BLAST of Lag0030831 vs. ExPASy Swiss-Prot
Match: O81825 (Probable disease resistance protein At4g27220 OS=Arabidopsis thaliana OX=3702 GN=At4g27220 PE=2 SV=1)

HSP 1 Score: 99.8 bits (247), Expect = 4.3e-20
Identity = 62/185 (33.51%), Postives = 97/185 (52.43%), Query Frame = 0

Query: 10  DVAINDLQEDEAWSLFKKFTGEIIETSDWKPFAVEIIAKCARLPVVITTVAKALRNRPSY 69
           ++ +  LQE EAW LF    GE+  + + KP A ++  +C  LP+ I T+ + LR +P  
Sbjct: 266 NIKVACLQEKEAWELFCHNVGEVANSDNVKPIAKDVSHECCGLPLAIITIGRTLRGKPQV 325

Query: 70  -IWKDALDQLRK--PVVVNIKGMKANVYSPLKLSYERLEDEEAKLLFLLCSMFPEDHSID 129
            +WK  L+ L++  P +      +  ++  LKLSY+ L+D   K  FL C++FPED+SI 
Sbjct: 326 EVWKHTLNLLKRSAPSI----DTEEKIFGTLKLSYDFLQD-NMKSCFLFCALFPEDYSIK 385

Query: 130 VEDLHVYAMCLDFLQDISTMAQGRRRIRKLVDNLISSSMLLSSKYNNHVKMHDIIRDVAI 189
           V +L +Y +    L               LV+ L  S +L      + VKMHD++RD AI
Sbjct: 386 VSELIMYWVAEGLLDGQHHYEDMMNEGVTLVERLKDSCLLEDGDSCDTVKMHDVVRDFAI 445

Query: 190 SIAST 192
              S+
Sbjct: 446 WFMSS 445

BLAST of Lag0030831 vs. ExPASy Swiss-Prot
Match: Q9FG91 (Probable disease resistance protein At5g43730 OS=Arabidopsis thaliana OX=3702 GN=At5g43730 PE=2 SV=1)

HSP 1 Score: 95.9 bits (237), Expect = 6.1e-19
Identity = 59/183 (32.24%), Postives = 98/183 (53.55%), Query Frame = 0

Query: 11  VAINDLQEDEAWSLFKKFTGEIIETS--DWKPFAVEIIAKCARLPVVITTVAKALRNRPS 70
           + ++ L  DEAW LF+   G+II  S  D    A  + AKC  LP+ +  + KA+  + +
Sbjct: 303 IKVDCLSPDEAWELFRLTVGDIILRSHQDIPALARIVAAKCHGLPLALNVIGKAMVCKET 362

Query: 71  -YIWKDALDQLRKPVVVNIKGMKANVYSPLKLSYERLEDEEAKLLFLLCSMFPEDHSIDV 130
              W+ A++ L  P      GM+  +   LK SY+ L++ E KL FL CS+FPED  I+ 
Sbjct: 363 VQEWRHAINVLNSP-GHKFPGMEERILPILKFSYDSLKNGEIKLCFLYCSLFPEDFEIEK 422

Query: 131 EDLHVYAMCLDFLQDISTMAQGRRRIRKLVDNLISSSMLLSSKYNNHVKMHDIIRDVAIS 190
           + L  Y +C  ++        G  +   ++  L+ + +L+  +  + VKMHD+IR++A+ 
Sbjct: 423 DKLIEYWICEGYINPNRYEDGGTNQGYDIIGLLVRAHLLIECELTDKVKMHDVIREMALW 482

BLAST of Lag0030831 vs. ExPASy Swiss-Prot
Match: P60839 (Probable disease resistance protein At1g12290 OS=Arabidopsis thaliana OX=3702 GN=At1g12290 PE=3 SV=1)

HSP 1 Score: 92.8 bits (229), Expect = 5.2e-18
Identity = 64/205 (31.22%), Postives = 102/205 (49.76%), Query Frame = 0

Query: 16  LQEDEAWSLFKKFTGEIIETS--DWKPFAVEIIAKCARLPVVITTVAKALR-NRPSYIWK 75
           L  D+AW LFKK  G+I  +S  D    A ++   C  LP+ +  + + +   + +  W 
Sbjct: 312 LGADDAWDLFKKKVGDITLSSHPDIPEIARKVAQACCGLPLALNVIGETMACKKTTQEWD 371

Query: 76  DALDQLRKPVVVNIKGMKANVYSPLKLSYERLEDEEAKLLFLLCSMFPEDHSIDVEDLHV 135
            A+D +      N   +K  +   LK SY+ LE E  K  FL CS+FPED  I+ E L  
Sbjct: 372 RAVD-VSTTYAANFGAVKERILPILKYSYDNLESESVKTCFLYCSLFPEDDLIEKERLID 431

Query: 136 YAMCLDFLQDISTMAQGRRRIRKLVDNLISSSMLL-SSKYNN--HVKMHDIIRDVAISIA 195
           Y +C  F+              +++  L+ +S+L+   K+NN  +VKMHD++R++A+ IA
Sbjct: 432 YWICEGFIDGDENKKGAVGEGYEILGTLVCASLLVEGGKFNNKSYVKMHDVVREMALWIA 491

Query: 196 STHDHIRTLCYLKKELNNNEGKKRK 215
           S     +  C ++     NE  K K
Sbjct: 492 SDLRKHKDNCIVRAGFRLNEIPKVK 515

BLAST of Lag0030831 vs. ExPASy TrEMBL
Match: A0A0A0LN08 (NB-ARC domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G096930 PE=4 SV=1)

HSP 1 Score: 227.3 bits (578), Expect = 6.5e-56
Identity = 120/204 (58.82%), Postives = 150/204 (73.53%), Query Frame = 0

Query: 13  INDLQEDEAWSLFKKFTGEIIETSDWKPFAVEIIAKCARLPVVITTVAKALRNRPSYIWK 72
           I  LQEDE W+LF+K  GEI+ETSD+K  AVEI+ +CA LP+ ITT+A+ALRN+P+ IWK
Sbjct: 302 IKFLQEDETWNLFRKMAGEIVETSDFKSIAVEIVRECAHLPIAITTIARALRNKPASIWK 361

Query: 73  DALDQLRKPVVVNIKGMKANVYSPLKLSYERLEDEEAKLLFLLCSMFPEDHSIDVEDLHV 132
           DAL QLR PV VNI+ +   VYS LKLSY+ L+ EEAK LFLLCSMFPED+ ID + LHV
Sbjct: 362 DALIQLRNPVFVNIREINKKVYSSLKLSYDYLDSEEAKSLFLLCSMFPEDYIIDCQVLHV 421

Query: 133 YAMCLDFLQDISTMAQGRRRIRKLVDNLISSSMLLSSKYNN---HVKMHDIIRDVAISIA 192
           YAM +  L  + ++AQ R RI KLVD+LISSS+LL     +   +VKMHDI+RDVAI IA
Sbjct: 422 YAMGMGLLHGVESVAQARNRITKLVDDLISSSLLLKESNVDLVMYVKMHDIVRDVAIIIA 481

Query: 193 STHDHIRTLCYLKKELNNNEGKKR 214
           S  D I TL Y K  L+ +  +K+
Sbjct: 482 SKDDRIFTLSYSKGLLDESWDEKK 505

BLAST of Lag0030831 vs. ExPASy TrEMBL
Match: A0A0A0LLJ0 (NB-ARC domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G014830 PE=4 SV=1)

HSP 1 Score: 226.5 bits (576), Expect = 1.1e-55
Identity = 123/206 (59.71%), Postives = 155/206 (75.24%), Query Frame = 0

Query: 13  INDLQEDEAWSLFKKFTGEIIETSDWKPFAVEIIAKCARLPVVITTVAKALRNRPSYIWK 72
           I  L EDE+W+LFK   GEI+E +D KP A++I+ +CA LP+ ITTVAKAL N+PS IW 
Sbjct: 306 IKVLGEDESWNLFKAMAGEIVEATDLKPIAIQIMRECAGLPIAITTVAKALLNKPSDIWN 365

Query: 73  DALDQLRKPVV--VNIKGMKANVYSPLKLSYERLEDEEAKLLFLLCSMFPEDHSIDVEDL 132
           DALDQL+   V   NI  M   VY  LKLSY+ L  EE KLLFLLCSMFPED +IDVE L
Sbjct: 366 DALDQLKSVDVGMANIGEMDKKVYLSLKLSYDYLGYEEVKLLFLLCSMFPEDFNIDVEKL 425

Query: 133 HVYAMCLDFLQDISTMAQGRRRIRKLVDNLISSSMLLS-SKY-NNHVKMHDIIRDVAISI 192
           H+YAM + FL+ + T+ +GRRRI+KLVD+LISSS+L   S+Y NN+VK+HD++RDVAI I
Sbjct: 426 HIYAMSMGFLRGVDTVVKGRRRIKKLVDDLISSSLLQQYSEYGNNYVKIHDMVRDVAILI 485

Query: 193 ASTHDHIRTLCYLKKELNNNEGKKRK 215
           AS +DHIRTL Y+K+  +N E K+ K
Sbjct: 486 ASQNDHIRTLSYVKR--SNEEWKEEK 509

BLAST of Lag0030831 vs. ExPASy TrEMBL
Match: A0A1S3B439 (probable disease resistance protein At4g27220 OS=Cucumis melo OX=3656 GN=LOC103485808 PE=4 SV=1)

HSP 1 Score: 224.9 bits (572), Expect = 3.2e-55
Identity = 122/194 (62.89%), Postives = 143/194 (73.71%), Query Frame = 0

Query: 13  INDLQEDEAWSLFKKFTGEIIETSDWKPFAVEIIAKCARLPVVITTVAKALRNRPSYIWK 72
           I  LQEDE W LFKK  GEI+ETSD +  AVEI  +CARLP+ ITT+AK LRN+P  IWK
Sbjct: 303 IKVLQEDETWRLFKKMGGEIVETSDLRSIAVEIARECARLPIAITTLAKTLRNKPLSIWK 362

Query: 73  DALDQLRKPVVVNIKGMKANVYSPLKLSYERLEDEEAKLLFLLCSMFPEDHSI-DVEDLH 132
           DAL QL+ PVVVNI+GM   VYS LKLSY++L+ EEAKLL LLCSMFPED  I +VE LH
Sbjct: 363 DALTQLKNPVVVNIRGMNEKVYSSLKLSYDQLDCEEAKLLLLLCSMFPEDCIINNVEYLH 422

Query: 133 VYAMCLDFLQDISTMAQGRRRIRKLVDNLISSSMLLSSK---YNNHVKMHDIIRDVAISI 192
           VYAM + FL  + T+ Q R RI KLVD+LISSS+LL          V+MHD+IRD+AI I
Sbjct: 423 VYAMGMGFLYGVDTVTQARHRITKLVDDLISSSLLLKESTDGLGECVRMHDLIRDLAILI 482

Query: 193 ASTHDHIRTLCYLK 203
           AS  DHIRTL + K
Sbjct: 483 ASKDDHIRTLSFSK 496

BLAST of Lag0030831 vs. ExPASy TrEMBL
Match: A0A5D3DFP8 (Putative disease resistance protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold333G00910 PE=4 SV=1)

HSP 1 Score: 224.9 bits (572), Expect = 3.2e-55
Identity = 122/194 (62.89%), Postives = 143/194 (73.71%), Query Frame = 0

Query: 13  INDLQEDEAWSLFKKFTGEIIETSDWKPFAVEIIAKCARLPVVITTVAKALRNRPSYIWK 72
           I  LQEDE W LFKK  GEI+ETSD +  AVEI  +CARLP+ ITT+AK LRN+P  IWK
Sbjct: 303 IKVLQEDETWRLFKKMGGEIVETSDLRSIAVEIARECARLPIAITTLAKTLRNKPLSIWK 362

Query: 73  DALDQLRKPVVVNIKGMKANVYSPLKLSYERLEDEEAKLLFLLCSMFPEDHSI-DVEDLH 132
           DAL QL+ PVVVNI+GM   VYS LKLSY++L+ EEAKLL LLCSMFPED  I +VE LH
Sbjct: 363 DALTQLKNPVVVNIRGMNEKVYSSLKLSYDQLDCEEAKLLLLLCSMFPEDCIINNVEYLH 422

Query: 133 VYAMCLDFLQDISTMAQGRRRIRKLVDNLISSSMLLSSK---YNNHVKMHDIIRDVAISI 192
           VYAM + FL  + T+ Q R RI KLVD+LISSS+LL          V+MHD+IRD+AI I
Sbjct: 423 VYAMGMGFLYGVDTVTQARHRITKLVDDLISSSLLLKESTDGLGECVRMHDLIRDLAILI 482

Query: 193 ASTHDHIRTLCYLK 203
           AS  DHIRTL + K
Sbjct: 483 ASKDDHIRTLSFSK 496

BLAST of Lag0030831 vs. ExPASy TrEMBL
Match: A0A0A0LMT4 (NB-ARC domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G403680 PE=4 SV=1)

HSP 1 Score: 224.2 bits (570), Expect = 5.5e-55
Identity = 119/195 (61.03%), Postives = 149/195 (76.41%), Query Frame = 0

Query: 13  INDLQEDEAWSLFKKFTGEIIETSDWKPFAVEIIAKCARLPVVITTVAKALRNRPSYIWK 72
           I  L E+E+W+LFK   G+I+E SD KP A++++ +CA LP+ ITTVAKALRN+PS IW 
Sbjct: 303 IKVLGENESWNLFKAMAGKIVEASDLKPIAIQVVRECAGLPIAITTVAKALRNKPSDIWN 362

Query: 73  DALDQLRKPVV--VNIKGMKANVYSPLKLSYERLEDEEAKLLFLLCSMFPEDHSIDVEDL 132
           DALDQL+   V   NI  M   VY  LKLSY+ L  EE KLLFLLCSMFPED SID+E+L
Sbjct: 363 DALDQLKSVDVFMTNIGEMDKKVYLSLKLSYDCLGYEEVKLLFLLCSMFPEDFSIDMEEL 422

Query: 133 HVYAMCLDFLQDISTMAQGRRRIRKLVDNLISSSMLLS-SKYN-NHVKMHDIIRDVAISI 192
           HVYAM + FL  + T+ +GRRRI+KLVD+LISSS+L   S+Y  N+VKMHD++RDVAI I
Sbjct: 423 HVYAMGMGFLHGVDTVVKGRRRIKKLVDDLISSSLLQQYSEYGYNYVKMHDMVRDVAIFI 482

Query: 193 ASTHDHIRTLCYLKK 204
           AS +DHIRTL Y+K+
Sbjct: 483 ASKNDHIRTLSYVKR 497

BLAST of Lag0030831 vs. TAIR 10
Match: AT4G27190.1 (NB-ARC domain-containing disease resistance protein )

HSP 1 Score: 115.5 bits (288), Expect = 5.3e-26
Identity = 65/183 (35.52%), Postives = 106/183 (57.92%), Query Frame = 0

Query: 10  DVAINDLQEDEAWSLFKKFTGEIIETSDWKPFAVEIIAKCARLPVVITTVAKALRNRPSY 69
           DV ++ L E++AW LF K  G+++ +   +  A  +  +C  LP+ I TV  A+R + + 
Sbjct: 296 DVRVDCLLEEDAWELFCKNAGDVVRSDHVRKIAKAVSQECGGLPLAIITVGTAMRGKKNV 355

Query: 70  -IWKDALDQLRKPVVVNIKGMKANVYSPLKLSYERLEDEEAKLLFLLCSMFPEDHSIDVE 129
            +W   L +L K V   IK ++  ++ PLKLSY+ LED +AK  FLLC++FPED+SI+V 
Sbjct: 356 KLWNHVLSKLSKSVPW-IKSIEEKIFQPLKLSYDFLED-KAKFCFLLCALFPEDYSIEVT 415

Query: 130 DLHVYAMCLDFLQDISTMAQGRRRIRKLVDNLISSSMLLSSKYNNHVKMHDIIRDVAISI 189
           ++  Y M   F++++ +           V++L    +L      + VKMHD++RD AI I
Sbjct: 416 EVVRYWMAEGFMEELGSQEDSMNEGITTVESLKDYCLLEDGDRRDTVKMHDVVRDFAIWI 475

Query: 190 AST 192
            S+
Sbjct: 476 MSS 476

BLAST of Lag0030831 vs. TAIR 10
Match: AT1G15890.1 (Disease resistance protein (CC-NBS-LRR class) family )

HSP 1 Score: 103.2 bits (256), Expect = 2.7e-22
Identity = 63/209 (30.14%), Postives = 107/209 (51.20%), Query Frame = 0

Query: 10  DVAINDLQEDEAWSLFKKFTGEIIETS--DWKPFAVEIIAKCARLPVVITTVAKALRNRP 69
           ++ ++ L  DEAW LF+K  G I   S  D    A ++  KC  LP+ ++ + KA+ +R 
Sbjct: 304 EMKVDCLPPDEAWELFQKKVGPIPLQSHEDIPTLARKVAEKCCGLPLALSVIGKAMASRE 363

Query: 70  S-YIWKDALDQLRKPVVVNIKGMKANVYSPLKLSYERLEDEEAKLLFLLCSMFPEDHSID 129
           +   W+  +  L          M+  +   LK SY+ L+DE+ KL FL CS+FPED+ + 
Sbjct: 364 TVQEWQHVIHVLNSS-SHEFPSMEEKILPVLKFSYDDLKDEKVKLCFLYCSLFPEDYEVR 423

Query: 130 VEDLHVYAMCLDFLQDISTMAQGRRRIRKLVDNLISSSMLLSSKYNNHVKMHDIIRDVAI 189
            E+L  Y MC  F+           +   ++ +L+ + +L+  +    VKMHD+IR++A+
Sbjct: 424 KEELIEYWMCEGFIDGNEDEDGANNKGHDIIGSLVRAHLLMDGELTTKVKMHDVIREMAL 483

Query: 190 SIASTHDHIR---------TLCYLKKELN 207
            IAS     +          LC++ K++N
Sbjct: 484 WIASNFGKQKETLCVKPGVQLCHIPKDIN 511

BLAST of Lag0030831 vs. TAIR 10
Match: AT4G27220.1 (NB-ARC domain-containing disease resistance protein )

HSP 1 Score: 99.8 bits (247), Expect = 3.0e-21
Identity = 62/185 (33.51%), Postives = 97/185 (52.43%), Query Frame = 0

Query: 10  DVAINDLQEDEAWSLFKKFTGEIIETSDWKPFAVEIIAKCARLPVVITTVAKALRNRPSY 69
           ++ +  LQE EAW LF    GE+  + + KP A ++  +C  LP+ I T+ + LR +P  
Sbjct: 266 NIKVACLQEKEAWELFCHNVGEVANSDNVKPIAKDVSHECCGLPLAIITIGRTLRGKPQV 325

Query: 70  -IWKDALDQLRK--PVVVNIKGMKANVYSPLKLSYERLEDEEAKLLFLLCSMFPEDHSID 129
            +WK  L+ L++  P +      +  ++  LKLSY+ L+D   K  FL C++FPED+SI 
Sbjct: 326 EVWKHTLNLLKRSAPSI----DTEEKIFGTLKLSYDFLQD-NMKSCFLFCALFPEDYSIK 385

Query: 130 VEDLHVYAMCLDFLQDISTMAQGRRRIRKLVDNLISSSMLLSSKYNNHVKMHDIIRDVAI 189
           V +L +Y +    L               LV+ L  S +L      + VKMHD++RD AI
Sbjct: 386 VSELIMYWVAEGLLDGQHHYEDMMNEGVTLVERLKDSCLLEDGDSCDTVKMHDVVRDFAI 445

Query: 190 SIAST 192
              S+
Sbjct: 446 WFMSS 445

BLAST of Lag0030831 vs. TAIR 10
Match: AT5G43730.1 (Disease resistance protein (CC-NBS-LRR class) family )

HSP 1 Score: 95.9 bits (237), Expect = 4.4e-20
Identity = 59/183 (32.24%), Postives = 98/183 (53.55%), Query Frame = 0

Query: 11  VAINDLQEDEAWSLFKKFTGEIIETS--DWKPFAVEIIAKCARLPVVITTVAKALRNRPS 70
           + ++ L  DEAW LF+   G+II  S  D    A  + AKC  LP+ +  + KA+  + +
Sbjct: 303 IKVDCLSPDEAWELFRLTVGDIILRSHQDIPALARIVAAKCHGLPLALNVIGKAMVCKET 362

Query: 71  -YIWKDALDQLRKPVVVNIKGMKANVYSPLKLSYERLEDEEAKLLFLLCSMFPEDHSIDV 130
              W+ A++ L  P      GM+  +   LK SY+ L++ E KL FL CS+FPED  I+ 
Sbjct: 363 VQEWRHAINVLNSP-GHKFPGMEERILPILKFSYDSLKNGEIKLCFLYCSLFPEDFEIEK 422

Query: 131 EDLHVYAMCLDFLQDISTMAQGRRRIRKLVDNLISSSMLLSSKYNNHVKMHDIIRDVAIS 190
           + L  Y +C  ++        G  +   ++  L+ + +L+  +  + VKMHD+IR++A+ 
Sbjct: 423 DKLIEYWICEGYINPNRYEDGGTNQGYDIIGLLVRAHLLIECELTDKVKMHDVIREMALW 482

BLAST of Lag0030831 vs. TAIR 10
Match: AT1G51480.1 (Disease resistance protein (CC-NBS-LRR class) family )

HSP 1 Score: 92.8 bits (229), Expect = 3.7e-19
Identity = 56/183 (30.60%), Postives = 95/183 (51.91%), Query Frame = 0

Query: 11  VAINDLQEDEAWSLFKKFTGEIIETS--DWKPFAVEIIAKCARLPVVITTVAKALRNRPS 70
           + ++ L  DEAW LF+    ++I +S  D    A  + AKC  LP+ +  + +A+  + +
Sbjct: 391 IKVSCLSPDEAWELFRITVDDVILSSHEDIPALARIVAAKCHGLPLALIVIGEAMACKET 450

Query: 71  -YIWKDALDQLRKPVVVNIKGMKANVYSPLKLSYERLEDEEAKLLFLLCSMFPEDHSIDV 130
              W  A++ L  P      GM+  +   LK SY+ L++ E KL FL CS+FPED  I+ 
Sbjct: 451 IQEWHHAINVLNSPAGHKFPGMEERILLVLKFSYDSLKNGEIKLCFLYCSLFPEDFEIEK 510

Query: 131 EDLHVYAMCLDFLQDISTMAQGRRRIRKLVDNLISSSMLLSSKYNNHVKMHDIIRDVAIS 190
           E L  Y +C  ++        G  +   ++  L+ + +L+  +    VKMH +IR++A+ 
Sbjct: 511 EKLIEYWICEGYINPNRYEDGGTNQGYDIIGLLVRAHLLIECELTTKVKMHYVIREMALW 570

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038890457.18.2e-6163.86probable disease resistance protein At4g27220 isoform X2 [Benincasa hispida][more]
XP_038890456.18.2e-6163.86probable disease resistance protein At4g27220 isoform X1 [Benincasa hispida][more]
XP_038890450.14.2e-5764.43disease resistance protein At4g27190-like isoform X1 [Benincasa hispida] >XP_038... [more]
XP_038890455.14.2e-5764.43disease resistance protein At4g27190-like isoform X3 [Benincasa hispida][more]
XP_038890454.14.2e-5764.43disease resistance protein At4g27190-like isoform X2 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
Q9T0487.5e-2535.52Disease resistance protein At4g27190 OS=Arabidopsis thaliana OX=3702 GN=At4g2719... [more]
Q9LMP63.9e-2130.14Probable disease resistance protein At1g15890 OS=Arabidopsis thaliana OX=3702 GN... [more]
O818254.3e-2033.51Probable disease resistance protein At4g27220 OS=Arabidopsis thaliana OX=3702 GN... [more]
Q9FG916.1e-1932.24Probable disease resistance protein At5g43730 OS=Arabidopsis thaliana OX=3702 GN... [more]
P608395.2e-1831.22Probable disease resistance protein At1g12290 OS=Arabidopsis thaliana OX=3702 GN... [more]
Match NameE-valueIdentityDescription
A0A0A0LN086.5e-5658.82NB-ARC domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G096930 PE=4... [more]
A0A0A0LLJ01.1e-5559.71NB-ARC domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G014830 PE=4... [more]
A0A1S3B4393.2e-5562.89probable disease resistance protein At4g27220 OS=Cucumis melo OX=3656 GN=LOC1034... [more]
A0A5D3DFP83.2e-5562.89Putative disease resistance protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5... [more]
A0A0A0LMT45.5e-5561.03NB-ARC domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G403680 PE=4... [more]
Match NameE-valueIdentityDescription
AT4G27190.15.3e-2635.52NB-ARC domain-containing disease resistance protein [more]
AT1G15890.12.7e-2230.14Disease resistance protein (CC-NBS-LRR class) family [more]
AT4G27220.13.0e-2133.51NB-ARC domain-containing disease resistance protein [more]
AT5G43730.14.4e-2032.24Disease resistance protein (CC-NBS-LRR class) family [more]
AT1G51480.13.7e-1930.60Disease resistance protein (CC-NBS-LRR class) family [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR042197Apoptotic protease-activating factors, helical domainGENE3D1.10.8.430coord: 15..100
e-value: 1.0E-14
score: 55.8
IPR002182NB-ARCPFAMPF00931NB-ARCcoord: 12..102
e-value: 2.3E-7
score: 30.3
IPR036388Winged helix-like DNA-binding domain superfamilyGENE3D1.10.10.10coord: 101..187
e-value: 1.1E-9
score: 40.2
NoneNo IPR availablePANTHERPTHR33463:SF49NB-ARC DOMAIN DISEASE RESISTANCE PROTEINcoord: 11..207
NoneNo IPR availablePANTHERPTHR33463FAMILY NOT NAMEDcoord: 11..207
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILY52540P-loop containing nucleoside triphosphate hydrolasescoord: 13..116

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0030831.1Lag0030831.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0043531 ADP binding