Lag0030750 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0030750 vs. NCBI nr
Match: KAF9671189.1 (hypothetical protein SADUNF_Sadunf12G0021600 [Salix dunnii])

HSP 1 Score: 158.7 bits (400), Expect = 3.6e-35
Identity = 76/131 (58.02%), Postives = 93/131 (70.99%), Query Frame = 0

Query: 4   LAQALKDM-DPKGVQLGAAPKCQFPDGHLGAAINTGLFDYVWVRFYDSPSRQFNGSSLDN 63
           LA+AL      K V L AAP+C FPD HL  AI TGLFDYVWV+FY++P  Q++G++ +N
Sbjct: 162 LARALNGFSQQKKVYLAAAPQCPFPDAHLDTAIKTGLFDYVWVQFYNNPPCQYSGNA-NN 221

Query: 64  LFNAWNFWQVIPAKEVFLGLAADPAAAAEGGFIPTKLVVSDVLPQITDSALYGGVMLWSK 123
           L +AW  W  + A +VFLGL ADPAAA  GGFIP  ++ S VLP I  S  YGGVMLWSK
Sbjct: 222 LLSAWKQWTTVQANQVFLGLPADPAAAPSGGFIPADVLTSQVLPSIKSSPKYGGVMLWSK 281

Query: 124 EFDHGYSDEIK 134
           +FD+GYS  IK
Sbjct: 282 QFDNGYSSAIK 291

BLAST of Lag0030750 vs. NCBI nr
Match: KAF8412165.1 (hypothetical protein HHK36_000123 [Tetracentron sinense])

HSP 1 Score: 158.7 bits (400), Expect = 3.6e-35
Identity = 74/134 (55.22%), Postives = 95/134 (70.90%), Query Frame = 0

Query: 3   QLAQALKDMDPKGVQLGAAPKCQFPDGHLGAAINTGLFDYVWVRFYDSPSRQFNGSSLDN 62
           +LA+AL     + V L AAP+C FPD HL  AI+TGLFDYVWV+FY++P  Q++G++ DN
Sbjct: 159 ELAKALAGFTQQKVYLAAAPQCPFPDAHLSGAISTGLFDYVWVQFYNNPPCQYSGNA-DN 218

Query: 63  LFNAWNFWQVIPAKEVFLGLAADPAAAAEGGFIPTKLVVSDVLPQITDSALYGGVMLWSK 122
           L +AWN W  + A ++FLGL A   AA  GGFIP  ++ S VLP I  S+ YGGVMLWSK
Sbjct: 219 LISAWNQWTTVQASQIFLGLPAAAEAAPSGGFIPADVLASQVLPTIKGSSKYGGVMLWSK 278

Query: 123 EFDHGYSDEIKDFV 137
           +FD+GYS  IK  V
Sbjct: 279 KFDNGYSSAIKSSV 291

BLAST of Lag0030750 vs. NCBI nr
Match: QEE82233.1 (basic endochitinase [Siraitia grosvenorii])

HSP 1 Score: 158.7 bits (400), Expect = 3.6e-35
Identity = 74/134 (55.22%), Postives = 93/134 (69.40%), Query Frame = 0

Query: 4   LAQALKDM-DPKGVQLGAAPKCQFPDGHLGAAINTGLFDYVWVRFYDSPSRQFNGSSLDN 63
           LA+AL    + + V L  AP+C FPD HL AAINTGLFDYVWV+FY++P  Q+   + +N
Sbjct: 163 LARALNGFNNQRKVFLATAPQCPFPDAHLNAAINTGLFDYVWVQFYNNPPCQYASGNANN 222

Query: 64  LFNAWNFWQVIPAKEVFLGLAADPAAAAEGGFIPTKLVVSDVLPQITDSALYGGVMLWSK 123
           + N+W  W  +PAKE+FLGL A P AA  GGFIP  +++S +LP I  S  YGGVMLWSK
Sbjct: 223 VLNSWRRWITVPAKELFLGLPAAPNAAPSGGFIPANVLISQILPTIKGSPKYGGVMLWSK 282

Query: 124 EFDHGYSDEIKDFV 137
            FD+GYS  IK  V
Sbjct: 283 AFDNGYSSAIKSSV 296

BLAST of Lag0030750 vs. NCBI nr
Match: CAB4271535.1 (unnamed protein product [Prunus armeniaca])

HSP 1 Score: 158.3 bits (399), Expect = 4.7e-35
Identity = 77/134 (57.46%), Postives = 92/134 (68.66%), Query Frame = 0

Query: 3   QLAQALKDMDPKGVQLGAAPKCQFPDGHLGAAINTGLFDYVWVRFYDSPSRQFNGSSLDN 62
           +LA++L     K V L AAP+C FPD HL  AI TGLFDYVWV+FY++P  QF  ++  N
Sbjct: 165 ELARSLSGRASK-VYLAAAPQCPFPDAHLDGAIKTGLFDYVWVQFYNNPPCQFADNNAAN 224

Query: 63  LFNAWNFWQVIPAKEVFLGLAADPAAAAEGGFIPTKLVVSDVLPQITDSALYGGVMLWSK 122
           L +AWN W    AK+VFLGL A P AA  GGFIP   + S VLP I  S  YGGVMLWSK
Sbjct: 225 LLSAWNQWTSTEAKQVFLGLPAAPEAAPSGGFIPADALKSQVLPNIKSSPKYGGVMLWSK 284

Query: 123 EFDHGYSDEIKDFV 137
           ++D+GYSD IKD V
Sbjct: 285 QYDNGYSDSIKDSV 297

BLAST of Lag0030750 vs. NCBI nr
Match: KAG6753741.1 (hypothetical protein POTOM_041739 [Populus tomentosa])

HSP 1 Score: 157.1 bits (396), Expect = 1.0e-34
Identity = 76/134 (56.72%), Postives = 95/134 (70.90%), Query Frame = 0

Query: 4   LAQALKDM-DPKGVQLGAAPKCQFPDGHLGAAINTGLFDYVWVRFYDSPSRQFNGSSLDN 63
           LA+AL      K V L AAP+C FPD HL  AI TGLFDYVWV+FY++P  Q++G++ +N
Sbjct: 162 LARALNGFSQQKKVHLAAAPQCPFPDAHLDTAIKTGLFDYVWVQFYNNPPCQYSGNA-NN 221

Query: 64  LFNAWNFWQVIPAKEVFLGLAADPAAAAEGGFIPTKLVVSDVLPQITDSALYGGVMLWSK 123
           L +AWN W  + A +VFLGL A PAAA  GGFIP  ++ S VLP I +S  YGGVMLW+K
Sbjct: 222 LLSAWNQWTTVQANQVFLGLPAAPAAAPSGGFIPADVLTSQVLPSIKNSPKYGGVMLWNK 281

Query: 124 EFDHGYSDEIKDFV 137
           +FD+GYS  IK  V
Sbjct: 282 QFDNGYSAAIKGSV 294

BLAST of Lag0030750 vs. ExPASy Swiss-Prot
Match: P36908 (Acidic endochitinase OS=Cicer arietinum OX=3827 PE=2 SV=1)

HSP 1 Score: 144.4 bits (363), Expect = 9.2e-34
Identity = 68/136 (50.00%), Postives = 90/136 (66.18%), Query Frame = 0

Query: 3   QLAQALKDMDPKGVQLGAAPKCQFPDGHLGAAINTGLFDYVWVRFYDSPSRQFNGSSLDN 62
           +LA+AL     + V L AAP+C +PD HL +AI TGLFDYVWV+FY++P  Q++  +++N
Sbjct: 158 ELAKALNGFSQQKVYLSAAPQCPYPDAHLDSAIQTGLFDYVWVQFYNNPQCQYSNGNINN 217

Query: 63  LFNAWNFWQVIPAKEVFLGLAADPAAAAEGGFIPTKLVVSDVLPQITDSALYGGVMLWSK 122
           L NAWN W    AK+VFLG+ A  AAA  GG IP  ++ S VLP I  S  YGGVM+W +
Sbjct: 218 LVNAWNQWTSSQAKQVFLGVPASDAAAPSGGLIPADVLTSQVLPAIKTSPKYGGVMIWDR 277

Query: 123 --EFDHGYSDEIKDFV 137
             +   GYS+ IK  V
Sbjct: 278 FNDAQSGYSNAIKGSV 293

BLAST of Lag0030750 vs. ExPASy Swiss-Prot
Match: P17541 (Acidic endochitinase OS=Cucumis sativus OX=3659 PE=1 SV=1)

HSP 1 Score: 143.3 bits (360), Expect = 2.0e-33
Identity = 70/130 (53.85%), Postives = 87/130 (66.92%), Query Frame = 0

Query: 4   LAQALKDMDPKGVQLGAAPKCQFPDGHLGAAINTGLFDYVWVRFYDSPSRQFNGSSLDNL 63
           LAQ LK+     V L AAP+C  PD HL AAI TGLFD VWV+FY++P   F   + DNL
Sbjct: 162 LAQELKNFGQ--VILSAAPQCPIPDAHLDAAIKTGLFDSVWVQFYNNPPCMF-ADNADNL 221

Query: 64  FNAWNFWQVIPAKEVFLGLAADPAAAAEGGFIPTKLVVSDVLPQITDSALYGGVMLWSKE 123
            ++WN W   P  ++++GL A   AA  GGFIP  +++S VLP I  S+ YGGVMLWSK 
Sbjct: 222 LSSWNQWTAFPTSKLYMGLPAAREAAPSGGFIPADVLISQVLPTIKASSNYGGVMLWSKA 281

Query: 124 FDHGYSDEIK 134
           FD+GYSD IK
Sbjct: 282 FDNGYSDSIK 288

BLAST of Lag0030750 vs. ExPASy Swiss-Prot
Match: P29060 (Acidic endochitinase OS=Nicotiana tabacum OX=4097 PE=1 SV=1)

HSP 1 Score: 142.5 bits (358), Expect = 3.5e-33
Identity = 71/132 (53.79%), Postives = 89/132 (67.42%), Query Frame = 0

Query: 3   QLAQALKDM-DPKGVQLGAAPKCQFPDGHLGAAINTGLFDYVWVRFYDSPSRQFNGSSLD 62
           +LA+ L      + V L AAP+C FPD  L  A++TGLFDYVWV+FY++P  Q++G S D
Sbjct: 158 ELAKTLSQFSQQRKVYLTAAPQCPFPDTWLNGALSTGLFDYVWVQFYNNPPCQYSGGSAD 217

Query: 63  NLFNAWNFWQVIPAKEVFLGLAADPAAAAEGGFIPTKLVVSDVLPQITDSALYGGVMLWS 122
           NL N WN W  I A ++FLGL A   AA   GFIP+ ++VS VLP I  S  YGGVMLWS
Sbjct: 218 NLKNYWNQWNAIQAGKIFLGLPAAQGAAG-SGFIPSDVLVSQVLPLINGSPKYGGVMLWS 277

Query: 123 KEFDHGYSDEIK 134
           K +D+GYS  IK
Sbjct: 278 KFYDNGYSSAIK 288

BLAST of Lag0030750 vs. ExPASy Swiss-Prot
Match: P23472 (Hevamine-A OS=Hevea brasiliensis OX=3981 PE=1 SV=2)

HSP 1 Score: 136.7 bits (343), Expect = 1.9e-31
Identity = 65/126 (51.59%), Postives = 89/126 (70.63%), Query Frame = 0

Query: 14  KGVQLGAAPKCQFPDGHLGAAINTGLFDYVWVRFYDSPSRQFNGSSLDNLFNAWNFWQV- 73
           K V L AAP+C FPD +LG A+NTGLFDYVWV+FY++P  Q++  +++N+ N+WN W   
Sbjct: 175 KKVYLTAAPQCPFPDRYLGTALNTGLFDYVWVQFYNNPPCQYSSGNINNIINSWNRWTTS 234

Query: 74  IPAKEVFLGLAADPAAAAEGGFIPTKLVVSDVLPQITDSALYGGVMLWSKEFD--HGYSD 133
           I A ++FLGL A P AA   G++P  +++S +LP+I  S  YGGVMLWSK +D  +GYS 
Sbjct: 235 INAGKIFLGLPAAPEAAG-SGYVPPDVLISRILPEIKKSPKYGGVMLWSKFYDDKNGYSS 294

Query: 134 EIKDFV 137
            I D V
Sbjct: 295 SILDSV 299

BLAST of Lag0030750 vs. ExPASy Swiss-Prot
Match: P36910 (Acidic endochitinase SE2 OS=Beta vulgaris OX=161934 GN=SE2 PE=1 SV=1)

HSP 1 Score: 136.3 bits (342), Expect = 2.5e-31
Identity = 67/123 (54.47%), Postives = 81/123 (65.85%), Query Frame = 0

Query: 14  KGVQLGAAPKCQFPDGHLGAAINTGLFDYVWVRFYDSPSRQFNGSSLDNLFNAWNFWQVI 73
           K V L AAP+C  PD  L  AI TGLFDYVWV+FY++P  Q++ +S DNL ++WN W  +
Sbjct: 173 KTVYLSAAPQCPLPDASLSTAIATGLFDYVWVQFYNNPPCQYD-TSADNLLSSWNQWTTV 232

Query: 74  PAKEVFLGLAADPAAAAEGGFIPTKLVVSDVLPQITDSALYGGVMLWSKEFDHGYSDEIK 133
            A ++FLGL A   AA   GFIP   + S VLP I  SA YGGVMLWSK +D GYS  IK
Sbjct: 233 QANQIFLGLPASTDAAG-SGFIPADALTSQVLPTIKGSAKYGGVMLWSKAYDSGYSSAIK 292

Query: 134 DFV 137
             V
Sbjct: 293 SSV 293