Lag0030744 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0030744
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
Descriptionsirohydrochlorin ferrochelatase, chloroplastic
Locationchr11: 994874 .. 999409 (+)
RNA-Seq ExpressionLag0030744
SyntenyLag0030744
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCCTTGGTCAATGAAACCTACAGTGCACATGTATTATCCCTTAGGAATCAGGTGCTAAACGGGTTAGCAAGTTTAGACCAACTAGTCTTACTACAGGCGTTTATAAGCTGATCATTGAAGTTATTGCTGAACGTGTTATTACAAGGTGTTGCCAATCACCATTACTAATTTTTAATCAACTTTCCTAGGTGGAAAAAAATTATTAGTTCGTGTTGTTTTGTTAATGAGCTGATCGATGATTGGAAAAGCAAAGGGAAAGAAGGATTGATTCTTGAACTAGATTTTGAAAATGCTTTGATTGAGTTGATTAGTGTCTCTATTAGGTATCCAAACTGTAACATCCCCAATTTAAATTAAACTCTTGTTTCTCAAATTACAAGTGTAATTGAGAAAATTTTCAATTAATTGGTTGAACCCTAATACTCTTTTAAGCTCAATGCACGCAGCCCAAGGAGGTTAAAAAAAAGAGTTTCATCTTCTTTCTCCATCTCCTTCTCCTTCAACCAAAAGACCCATCCTCCATAGCCTTCTTCTTCTTTTGCCATAGCCATCGGCCAAACCGTTCTCCTCCATCTCCGACTGTCTTCTCCGGCAACAACAAAACAGAGGCGGTGGTAGTTTCCGAGGAGTCCTTCGCGCGGCCAACAACGTCTTGTGTGTGGGTAAGTGTTTTCTTTCCTCGATTTTTCTTGTTTCTCAAGCCGATCTCCCTCCCTTTTCGTTCTCTCTCACTTTCGATCTCTCCCTCTCTGTTTTCCGGCGGATTTTAGGCATGGTAGTGGCTGGTTTCGCGTTTCTTTCGGCGGGTACAGTGTAGGCGCAGTTTTTTTTTTTTTTTTTTTAAGAGAAACAATTCATTGATAGATGAAATAAAGGATGCTAAAAAGCACCCCACGTTTAAAGACAATTACAATAAAGATCTCCAGCTAGTAATCAAAGTAGAGTGACTATATGCATTAAATGGGTGTTTATTTAGCAGCAGTTTGGGGCGTTTTTTAGTGTTTTTGGCGCGTTTTCAACATGGGTAAGTGAGATTTAAGGTGTTTTTGATCAATTTTATGTTTAATCAAGGATACCCATTGATGTTAGCTCTCAATTTCAAGGTACCCTGCCCATTGCGTTTAGTGATATATTCGTCAATTTGGAGTGAAATTTAAAGTTTGGACAGCAAGTATTTGTTTTGTTTTTGGGTAAGTTTTGGGTGTTAAATTCCCCAAATTTAATGAGCTCCTAGTAGAACAATTGATTTGAAATTCTATTTGGTTTTGAGGAAGTTTTCCCTTTTTGTGTAGCTTGCCTAAGGCCTTGGAGTTAAGGAATTTTGAAAGGATTTAGCAAATATTGTTTCGTAAAGGTAAGTAGCAATTTGGAATAAGTTCTATGCTTTAAACCCATTTTATTTGTGAATGGTTTATGCTAGCATGAATTGGATTTAATCTTTAAGTTGGCCTTTGAATTTAAGTAGCATATTGGGTTAATTAAAATTTCATACTAACATCATGCTCCAAAATAAACCTTAAAAGCATGTTGAGATTTGCCTTTAGTTGGGCGTGGTGCGAGTAGCACGGATTATGGGTTAATTGAGTTTGGGTTGAATTATTATTAATTCTAAAGCTTGCTTGTGATGTGAGAATGAATTTCATGTTCTATTTGGGTTAGTCACGAAGTGATTTGTTTTGAATGAATTTATGTTCGACTGATAATCTCTAAAGTGGTAGTAGATATGATTGAGCATGCAAAATATGGACTTTGTATTGGAATGTTAATGGCTAGAATTACTCTAGCTTAGAAGTGCGATTTTCTACTTTTCAGGTGACTACAATTTTTGGTATAGTTGGTTACGTCGTTATGCTATCGAATTTTCGGTAACTTTATAGTAGTTATTGGGGCTAAGTTTAGCAATGTCCTTTGGATTATGATGTGCTAATATTGACTGACCGATTAGTATAAACCACATAATATCCAAGCATGTTTTAGTATGACTTATTGAGAACCATATGAATGAATGGGAATAGGCGTAGAATAGGGACCATATCATGTTTTAGGGCCTTAGGCAGACTTAAAGGATCCTTACGTCTTATGTTAACTAAAGTGGTTTCCTTCCCCATCTCAAGACTGATAGAACCCAATTTTCAGGCATGTTATCGTGTGTTCTATCAGCTGACTGTTAGAGTCCAATTACAGGCACGTTATCGTGCGCTCTCTCAGTTCAGCATGGGATTGTTTTATTTAAATTTGTTCATTTTTTTCCCTCTTACTCTTTTACCTTTGATGTCAGCAAGAAGTTAACTAAATTTACTTCATAGTATACTCTCGTGACTCAGCACATGAAATCTAGATCAAGCATTAGAGTCATTGAGTTGAAATGATCAAATTTCTAAAATAAAGATCAATTTTACAATTTTCAATAAAAACATGTTAAGTTTGTAAAAACCACAAATTTTCTTTTATAGCCCACCACCCAGGCACCCACCAAGTATTTCAAACGACATTGTTTGCCAAGACAATTCTTCCCCTTAGGTTCAGAAGGCTATCTTCGATCATAACTCATGAGATCGTTATCCATCACTCCTCAAATTACAGTAAAGAGGTCTGAAAATCTTCTTAACTTCCAGTTTTTCAATTTGCTCAATCTGCCTCTAATGGCCGTTCCTTTCGCCATTGAAGTCCCTCAGCAGGCGAAACTAGGACATTCACAATCAGCGCATCTCAGAAATCTCCGATCCTGATCGGACGACCGTCGAGATTCAGGAATTTCACTTTGCGGTTCTGCATGAGTGTCGATAATGGAAGCCCTAATCGGAGCCGAGATCAGATTGGTGCCGGAGATGCCGTCATCATCGTCGACCATGGCTCTCGCCGTCGGGAATCTAATCTGATGTTGAGTAAGCTTGGGTTTTGGAATTTGGAATTTGGAATTAGGGTGAAATTTGAAGTTCTTCGTTGGCTAATCCTCTTGTGGTTCTGAATTTGTTCGAAAATTTCAGACGAGTTCGTTGCTATGTTTAGGGATAAAACTGGTTACCCTATTGTGGAGCCTGCTCACATGGTATGTTCTTCTTAACTGTATTTTTTTTTTTTTTTTTTTTGAGAAAATCTTCTTAACTTTATTTTTCACTTGGTTGAGCTACTAATTTCATCCTTATAGCTTGATATTTCTTTAATTTTGAGTTTTAAAACGTTTTAATTATTGCTTGAAATTTGAATTATTTTTATCTTTTGTCTCTATCTTTCAACAGCAGACTAACGCTGCATTTTATTTGATAAAATTTAAACGAGATCCAATATTTATTTATTTAATAAAATGTCATATTAACCTAGTTGATGTTAACATTAGGACTTGAAATAAACCACAATTCGCTAATTACGTAATCAAAAACTTTTTGATGTCAAACTAATTTTTCAGTTTGGTGGATGACCTTTCCTTCCAATTAAACGAGATTATTTGGAGGCTTTATTGGCCAAAATGAAGTGTTTGCCTTATTGTACTTTAGTTTATCTTCTTTCCCCACCCTATAAAAAGTGTGAGATTGATTTTTTTTTTTTAACCTTTTTGGAAATATTTCATGTGCCACTCTAAGGACGGACTAAAATGTCGATATTAAAGAATGTCTTTGATATTTTTCATACTTCATTGATATATTTACCATATTATCAAAAATACCAATCATCCTCAATATCAAGGTTGAATCTTTTGGTTTATGAAAATATTGATAGGTTGAGGCCTGAGGGGTATATTTTTTTTCCTACTCTTTGAAGTACAACATCTCTAGCCTTCAAAATACCATAGGATTCAGTGAATACAAAAACAAAATAAATGATGATATTCGGAAATATGAACAGTGGTATGGATTGGAATCGTACCACTAGAAGTTTCTTTGTCTTTTTCATTCCTTCCAGACTCTTCCCTTTACACATATGTTTCAACACACGATGTTACTTAGCTATGTAGCTATGTAAGTAGGGCAGGAGGGGCTCTAAAGATTTATCTTTTACTTTGTTCCAAAACAACACAGGAGCTGGCCGAACCGTCGATAAGAGACTCGTTTGCATCATGTGTCGAACAAGGGGCCAAACGAATCATTGTAAGCCCATTCTTCCTCTTCCCTGGTCGACATTGGCAACAGGTAACTCACTGTAAGATTAATGAACTTAATTGGTGGCCTTTGGATCTTCTATGATGACTCTTTAAGTAGGAGGATTGATAACAATGGCTGAAATTTTGCAGGACATTCCTTCCTTAACAGCTGAAGCAGCAAAGGAGCACCCTGGAATATCATATATAATAACTGCTCCTCTTGGTCTTCATGAACAACTAGTGGTAGGAATTTCTCAACTACATGTAGAGGACAGTAAAGAAAGCAATGTCATAAATGATAAAAAGAAAAGACTGTATTTGTTAGTATTGAAATGTGAGGTTTTCTGTTGGCAGGATGTTGTGAATGATAGAATAAATCACTGCTTAAGCCATGCTGCTGGAGTTGCAGATGAATGTGAAGTTTGCGCTGGAACAGGCAAATGCAGATTTAATTAG

mRNA sequence

ATGCCTTGGTCAATGAAACCTACAGTGCACATGTATTATCCCTTAGGAATCAGCCATCGGCCAAACCGTTCTCCTCCATCTCCGACTGTCTTCTCCGGCAACAACAAAACAGAGGCGGTGGTAGTTTCCGAGGAGTCCTTCGCGCGGCCAACAACGTCTTGTGTGTGGGTGACTACAATTTTTGGTATAGTTGGTTACGTCGTTATGCTATCGAATTTTCGTCCCTCAGCAGGCGAAACTAGGACATTCACAATCAGCGCATCTCAGAAATCTCCGATCCTGATCGGACGACCGTCGAGATTCAGGAATTTCACTTTGCGGTTCTGCATGAGTGTCGATAATGGAAGCCCTAATCGGAGCCGAGATCAGATTGGTGCCGGAGATGCCGTCATCATCGTCGACCATGGCTCTCGCCGTCGGGAATCTAATCTGATGTTGAACGAGTTCGTTGCTATGTTTAGGGATAAAACTGGTTACCCTATTGTGGAGCCTGCTCACATGGAGCTGGCCGAACCGTCGATAAGAGACTCGTTTGCATCATGTGTCGAACAAGGGGCCAAACGAATCATTGTAAGCCCATTCTTCCTCTTCCCTGGTCGACATTGGCAACAGGACATTCCTTCCTTAACAGCTGAAGCAGCAAAGGAGCACCCTGGAATATCATATATAATAACTGCTCCTCTTGGTCTTCATGAACAACTAGTGGATGTTGTGAATGATAGAATAAATCACTGCTTAAGCCATGCTGCTGGAGTTGCAGATGAATGTGAAGTTTGCGCTGGAACAGGCAAATGCAGATTTAATTAG

Coding sequence (CDS)

ATGCCTTGGTCAATGAAACCTACAGTGCACATGTATTATCCCTTAGGAATCAGCCATCGGCCAAACCGTTCTCCTCCATCTCCGACTGTCTTCTCCGGCAACAACAAAACAGAGGCGGTGGTAGTTTCCGAGGAGTCCTTCGCGCGGCCAACAACGTCTTGTGTGTGGGTGACTACAATTTTTGGTATAGTTGGTTACGTCGTTATGCTATCGAATTTTCGTCCCTCAGCAGGCGAAACTAGGACATTCACAATCAGCGCATCTCAGAAATCTCCGATCCTGATCGGACGACCGTCGAGATTCAGGAATTTCACTTTGCGGTTCTGCATGAGTGTCGATAATGGAAGCCCTAATCGGAGCCGAGATCAGATTGGTGCCGGAGATGCCGTCATCATCGTCGACCATGGCTCTCGCCGTCGGGAATCTAATCTGATGTTGAACGAGTTCGTTGCTATGTTTAGGGATAAAACTGGTTACCCTATTGTGGAGCCTGCTCACATGGAGCTGGCCGAACCGTCGATAAGAGACTCGTTTGCATCATGTGTCGAACAAGGGGCCAAACGAATCATTGTAAGCCCATTCTTCCTCTTCCCTGGTCGACATTGGCAACAGGACATTCCTTCCTTAACAGCTGAAGCAGCAAAGGAGCACCCTGGAATATCATATATAATAACTGCTCCTCTTGGTCTTCATGAACAACTAGTGGATGTTGTGAATGATAGAATAAATCACTGCTTAAGCCATGCTGCTGGAGTTGCAGATGAATGTGAAGTTTGCGCTGGAACAGGCAAATGCAGATTTAATTAG

Protein sequence

MPWSMKPTVHMYYPLGISHRPNRSPPSPTVFSGNNKTEAVVVSEESFARPTTSCVWVTTIFGIVGYVVMLSNFRPSAGETRTFTISASQKSPILIGRPSRFRNFTLRFCMSVDNGSPNRSRDQIGAGDAVIIVDHGSRRRESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFASCVEQGAKRIIVSPFFLFPGRHWQQDIPSLTAEAAKEHPGISYIITAPLGLHEQLVDVVNDRINHCLSHAAGVADECEVCAGTGKCRFN
Homology
[Close]
BLAST of Lag0030744 vs. NCBI nr
Match: XP_038890885.1 (sirohydrochlorin ferrochelatase, chloroplastic isoform X1 [Benincasa hispida])

HSP 1 Score: 357.1 bits (915), Expect = 1.4e-94
Identity = 177/194 (91.24%), Postives = 183/194 (94.33%), Query Frame = 0 

Query: 76  SAGET-RTFTISASQKSPILIGRPSRFRNFTLRFCMSVDNGSPNRSRDQIGAGDAVIIVD 135
           SA ET R +TI ASQKSPILI R SRFRNFT RF MSVDNGS NRS D+IGAGDAVIIVD
Sbjct: 53  SAVETRRKYTICASQKSPILIRRLSRFRNFTSRFRMSVDNGSSNRSGDRIGAGDAVIIVD 112

Query: 136 HGSRRRESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFASCVEQGAKRIIVSPF 195
           HGSRRRESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFA CVEQGAKRIIVSPF
Sbjct: 113 HGSRRRESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFALCVEQGAKRIIVSPF 172

Query: 196 FLFPGRHWQQDIPSLTAEAAKEHPGISYIITAPLGLHEQLVDVVNDRINHCLSHAAGVAD 255
           FLFPGRHWQQDIPSLTAEAAK+HPGISYIITAPLGLHE+LVDVVNDRI+HCLSHAAGVA+
Sbjct: 173 FLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEKLVDVVNDRISHCLSHAAGVAE 232

Query: 256 ECEVCAGTGKCRFN 269
           ECEVCAGTGKCRFN
Sbjct: 233 ECEVCAGTGKCRFN 246

[Close]
BLAST of Lag0030744 vs. NCBI nr
Match: KAG7033101.1 (Sirohydrochlorin ferrochelatase, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 349.7 bits (896), Expect = 2.2e-92
Identity = 167/201 (83.08%), Postives = 181/201 (90.05%), Query Frame = 0 

Query: 68  VMLSNFRPSAGETRTFTISASQKSPILIGRPSRFRNFTLRFCMSVDNGSPNRSRDQIGAG 127
           + + +F       RT+TIS SQKS I IGRPSRF NFT RF MSVDNG  ++SRD+IGAG
Sbjct: 10  ITVKSFSAGDARRRTYTISTSQKSAIPIGRPSRFGNFTSRFRMSVDNGGSSQSRDRIGAG 69

Query: 128 DAVIIVDHGSRRRESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFASCVEQGAK 187
           DAVIIVDHGSRR+ESNLMLN FVAMFRDKTGYPIVEPA MELA+PSIRD+F SCVEQGAK
Sbjct: 70  DAVIIVDHGSRRQESNLMLNAFVAMFRDKTGYPIVEPAPMELAKPSIRDAFKSCVEQGAK 129

Query: 188 RIIVSPFFLFPGRHWQQDIPSLTAEAAKEHPGISYIITAPLGLHEQLVDVVNDRINHCLS 247
           RII+SPFFLFPGRHWQQDIPSLTAEAAK+HPGISYI+TAPLGLHE+LVDVVNDRINHCLS
Sbjct: 130 RIIISPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIVTAPLGLHEKLVDVVNDRINHCLS 189

Query: 248 HAAGVADECEVCAGTGKCRFN 269
           HAAGVADECEVCAGTGKCRFN
Sbjct: 190 HAAGVADECEVCAGTGKCRFN 210

[Close]
BLAST of Lag0030744 vs. NCBI nr
Match: XP_023551685.1 (sirohydrochlorin ferrochelatase, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 347.1 bits (889), Expect = 1.4e-91
Identity = 173/197 (87.82%), Postives = 181/197 (91.88%), Query Frame = 0 

Query: 75  PSAGETR-TFTISASQKSPILI--GRPSRFRNFTLRFCMSVDNGSPNRSRDQIGAGDAVI 134
           PSAGETR T+TI ASQKSPILI  GR S  RNF+ RF MSVDNG      D+IGAGDAVI
Sbjct: 15  PSAGETRKTYTICASQKSPILIGVGRSSTLRNFSSRFRMSVDNG----GADRIGAGDAVI 74

Query: 135 IVDHGSRRRESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFASCVEQGAKRIIV 194
           IVDHGSRRRESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFASCVEQGAKRI+V
Sbjct: 75  IVDHGSRRRESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFASCVEQGAKRIVV 134

Query: 195 SPFFLFPGRHWQQDIPSLTAEAAKEHPGISYIITAPLGLHEQLVDVVNDRINHCLSHAAG 254
           SPFFLFPGRHWQQDIPSLTAEAAK+HPGISYIITAPLGLHEQLVDVVNDRI+HCLSHAAG
Sbjct: 135 SPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEQLVDVVNDRISHCLSHAAG 194

Query: 255 VADECEVCAGTGKCRFN 269
           V +ECEVCAGTGKC+FN
Sbjct: 195 VVEECEVCAGTGKCKFN 207

[Close]
BLAST of Lag0030744 vs. NCBI nr
Match: KAA0037556.1 (sirohydrochlorin ferrochelatase [Cucumis melo var. makuwa] >TYK03116.1 sirohydrochlorin ferrochelatase [Cucumis melo var. makuwa])

HSP 1 Score: 346.7 bits (888), Expect = 1.8e-91
Identity = 172/195 (88.21%), Postives = 179/195 (91.79%), Query Frame = 0 

Query: 75  PSAGETRT-FTISASQKSPILIGRPSRFRNFTLRFCMSVDNGSPNRSRDQIGAGDAVIIV 134
           PS  ETR+ +TI A Q+SPI IGR S+FRNFT R  MS+DNGS NR    IGAGDAVIIV
Sbjct: 15  PSPVETRSPYTICAFQRSPIPIGRLSKFRNFTSRVRMSIDNGSSNR----IGAGDAVIIV 74

Query: 135 DHGSRRRESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFASCVEQGAKRIIVSP 194
           DHGSRRRESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSF SCVEQGAKRIIVSP
Sbjct: 75  DHGSRRRESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFTSCVEQGAKRIIVSP 134

Query: 195 FFLFPGRHWQQDIPSLTAEAAKEHPGISYIITAPLGLHEQLVDVVNDRINHCLSHAAGVA 254
           FFLFPGRHWQQDIPSLTAEAAK+HPGISYIITAPLGLHE+LVDVVNDRINHCLSHAAGVA
Sbjct: 135 FFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEKLVDVVNDRINHCLSHAAGVA 194

Query: 255 DECEVCAGTGKCRFN 269
           DECEVCAGTGKCRFN
Sbjct: 195 DECEVCAGTGKCRFN 205

[Close]
BLAST of Lag0030744 vs. NCBI nr
Match: XP_008458790.1 (PREDICTED: sirohydrochlorin ferrochelatase, chloroplastic [Cucumis melo])

HSP 1 Score: 346.7 bits (888), Expect = 1.8e-91
Identity = 172/195 (88.21%), Postives = 179/195 (91.79%), Query Frame = 0 

Query: 75  PSAGETRT-FTISASQKSPILIGRPSRFRNFTLRFCMSVDNGSPNRSRDQIGAGDAVIIV 134
           PS  ETR+ +TI ASQ+SPI IGR S+FRNFT R  MS+DNGS NR    IGAGDAVIIV
Sbjct: 15  PSPVETRSPYTICASQRSPIPIGRLSKFRNFTSRVRMSIDNGSSNR----IGAGDAVIIV 74

Query: 135 DHGSRRRESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFASCVEQGAKRIIVSP 194
           DHGSRRRESN MLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSF SCVEQGAKRIIVSP
Sbjct: 75  DHGSRRRESNRMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFTSCVEQGAKRIIVSP 134

Query: 195 FFLFPGRHWQQDIPSLTAEAAKEHPGISYIITAPLGLHEQLVDVVNDRINHCLSHAAGVA 254
           FFLFPGRHWQQDIPSLTAEAAK+HPGISYIITAPLGLHE+LVDVVNDRINHCLSHAAGVA
Sbjct: 135 FFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEKLVDVVNDRINHCLSHAAGVA 194

Query: 255 DECEVCAGTGKCRFN 269
           DECEVCAGTGKCRFN
Sbjct: 195 DECEVCAGTGKCRFN 205

[Close]
BLAST of Lag0030744 vs. ExPASy Swiss-Prot
Match: Q84JH7 (Sirohydrochlorin ferrochelatase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=SIRB PE=1 SV=1)

HSP 1 Score: 242.3 bits (617), Expect = 6.4e-63
Identity = 111/146 (76.03%), Postives = 126/146 (86.30%), Query Frame = 0 

Query: 121 RDQIGAGDAVIIVDHGSRRRESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFAS 180
           ++ IG  D +IIVDHGSRRRESNLML EFV MF++KTGYPIVEPAHMELAEPSI+D+F+ 
Sbjct: 75  KNGIGDADGIIIVDHGSRRRESNLMLEEFVKMFKEKTGYPIVEPAHMELAEPSIKDAFSL 134

Query: 181 CVEQGAKRIIVSPFFLFPGRHWQQDIPSLTAEAAKEHPGISYIITAPLGLHEQLVDVVND 240
           CV+QGAKR++VSPFFLFPGRHW  DIPSLTA+AAKE  GISY+ITAPLG H  L+DVVND
Sbjct: 135 CVQQGAKRVVVSPFFLFPGRHWHTDIPSLTADAAKEFSGISYLITAPLGPHNLLLDVVND 194

Query: 241 RINHCLSHAAGVADECEVCAGTGKCR 267
           RI HCLSH  G ADEC VCAGT KC+
Sbjct: 195 RIQHCLSHVEGDADECLVCAGTNKCK 220

[Close]
BLAST of Lag0030744 vs. ExPASy Swiss-Prot
Match: P61817 (Sirohydrochlorin ferrochelatase OS=Bacillus megaterium OX=1404 GN=sirB PE=3 SV=1)

HSP 1 Score: 87.0 bits (214), Expect = 3.4e-16
Identity = 41/116 (35.34%), Postives = 62/116 (53.45%), Query Frame = 0 

Query: 128 DAVIIVDHGSRRRESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFASCVEQGAK 187
           DAV+ V HGSR +E       F+   +     PI E   +ELA P+I   F +C+EQGA 
Sbjct: 13  DAVLYVCHGSRVKEGADQAVAFIERCKKNLDVPIQEVCFLELASPTIEQGFEACIEQGAT 72

Query: 188 RIIVSPFFLFPGRHWQQDIPSLTAEAAKEHPGISYIITAPLGLHEQLVDVVNDRIN 244
           RI + P  L    H + DIP    +  + +P +  +   P G+ E++VD++ +RIN
Sbjct: 73  RIAIVPLLLLTAAHAKHDIPEEIQKVYERYPQVEVLYGEPFGVDERIVDILVERIN 128

[Close]
BLAST of Lag0030744 vs. ExPASy Swiss-Prot
Match: P61819 (Sirohydrochlorin cobaltochelatase OS=Methanococcus maripaludis (strain S2 / LL) OX=267377 GN=cbiX PE=3 SV=1)

HSP 1 Score: 77.8 bits (190), Expect = 2.1e-13
Identity = 41/141 (29.08%), Postives = 67/141 (47.52%), Query Frame = 0 

Query: 128 DAVIIVDHGSRRRESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFASCVEQGAK 187
           +A+++V HGSR   S  ++ E     + +  Y IVE   ME  EP+I  +    ++ GAK
Sbjct: 2   EALVLVGHGSRLPHSKNVVMEVAEKIKARNIYDIVEVGMMEFNEPTIPQTIKKVIDAGAK 61

Query: 188 RIIVSPFFLFPGRHWQQDIPSLTA------EAAKEH-------------------PGISY 244
           ++IV+P FL PG H ++DIP +        E    H                    G+  
Sbjct: 62  KVIVTPVFLAPGNHTERDIPKILGIYEGDDEGGHHHHHDHEHHHHHHDTTAVEIPEGVEL 121

[Close]
BLAST of Lag0030744 vs. ExPASy Swiss-Prot
Match: Q58380 (Sirohydrochlorin cobaltochelatase OS=Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) OX=243232 GN=cbiX PE=1 SV=1)

HSP 1 Score: 75.1 bits (183), Expect = 1.3e-12
Identity = 45/138 (32.61%), Postives = 67/138 (48.55%), Query Frame = 0 

Query: 128 DAVIIVDHGSRRRESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFASCVEQGAK 187
           +A+++V HGSR   S  +L +     +++  +PIVE   ME +EP+I  +    +EQGAK
Sbjct: 2   EALVLVGHGSRLPYSKELLVKLAEKVKERNLFPIVEIGLMEFSEPTIPQAVKKAIEQGAK 61

Query: 188 RIIVSPFFLFPGRHWQQDIPSLTA------EAAKEH------------------PGISYI 242
           RIIV P FL  G H  +DIP L        E   EH                    +  I
Sbjct: 62  RIIVVPVFLAHGIHTTRDIPRLLGLIEDNHEHHHEHSHHHHHHHHHEHEKLEIPEDVEII 121

[Close]
BLAST of Lag0030744 vs. ExPASy Swiss-Prot
Match: A6UWT5 (Sirohydrochlorin cobaltochelatase OS=Methanococcus aeolicus (strain ATCC BAA-1280 / DSM 17508 / OCM 812 / Nankai-3) OX=419665 GN=cbiX PE=3 SV=1)

HSP 1 Score: 70.9 bits (172), Expect = 2.5e-11
Identity = 40/138 (28.99%), Postives = 66/138 (47.83%), Query Frame = 0 

Query: 128 DAVIIVDHGSRRRESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFASCVEQGAK 187
           +A++++ HGSR   S  ++ +     ++K  Y IVE   ME  EP+I  +    + +GAK
Sbjct: 2   EALVLLGHGSRLPYSKEIVGKVAEKIKEKNIYDIVEIGMMEFNEPTIPQTINKVIAEGAK 61

Query: 188 RIIVSPFFLFPGRHWQQDIPSLTA--EAAKEH----------------------PGISYI 242
           +II+ P FL  G H ++DIP +    E  + H                       G+  I
Sbjct: 62  KIIIVPVFLAHGNHTKRDIPQILGLIECEEHHHEGEGGHHHHHHHHHGEKIEVPEGVEII 121

[Close]
BLAST of Lag0030744 vs. ExPASy TrEMBL
Match: A0A5D3BY55 (Sirohydrochlorin ferrochelatase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold374G00520 PE=4 SV=1)

HSP 1 Score: 346.7 bits (888), Expect = 8.9e-92
Identity = 172/195 (88.21%), Postives = 179/195 (91.79%), Query Frame = 0 

Query: 75  PSAGETRT-FTISASQKSPILIGRPSRFRNFTLRFCMSVDNGSPNRSRDQIGAGDAVIIV 134
           PS  ETR+ +TI A Q+SPI IGR S+FRNFT R  MS+DNGS NR    IGAGDAVIIV
Sbjct: 15  PSPVETRSPYTICAFQRSPIPIGRLSKFRNFTSRVRMSIDNGSSNR----IGAGDAVIIV 74

Query: 135 DHGSRRRESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFASCVEQGAKRIIVSP 194
           DHGSRRRESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSF SCVEQGAKRIIVSP
Sbjct: 75  DHGSRRRESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFTSCVEQGAKRIIVSP 134

Query: 195 FFLFPGRHWQQDIPSLTAEAAKEHPGISYIITAPLGLHEQLVDVVNDRINHCLSHAAGVA 254
           FFLFPGRHWQQDIPSLTAEAAK+HPGISYIITAPLGLHE+LVDVVNDRINHCLSHAAGVA
Sbjct: 135 FFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEKLVDVVNDRINHCLSHAAGVA 194

Query: 255 DECEVCAGTGKCRFN 269
           DECEVCAGTGKCRFN
Sbjct: 195 DECEVCAGTGKCRFN 205

[Close]
BLAST of Lag0030744 vs. ExPASy TrEMBL
Match: A0A1S3C8S9 (sirohydrochlorin ferrochelatase, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103498093 PE=4 SV=1)

HSP 1 Score: 346.7 bits (888), Expect = 8.9e-92
Identity = 172/195 (88.21%), Postives = 179/195 (91.79%), Query Frame = 0 

Query: 75  PSAGETRT-FTISASQKSPILIGRPSRFRNFTLRFCMSVDNGSPNRSRDQIGAGDAVIIV 134
           PS  ETR+ +TI ASQ+SPI IGR S+FRNFT R  MS+DNGS NR    IGAGDAVIIV
Sbjct: 15  PSPVETRSPYTICASQRSPIPIGRLSKFRNFTSRVRMSIDNGSSNR----IGAGDAVIIV 74

Query: 135 DHGSRRRESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFASCVEQGAKRIIVSP 194
           DHGSRRRESN MLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSF SCVEQGAKRIIVSP
Sbjct: 75  DHGSRRRESNRMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFTSCVEQGAKRIIVSP 134

Query: 195 FFLFPGRHWQQDIPSLTAEAAKEHPGISYIITAPLGLHEQLVDVVNDRINHCLSHAAGVA 254
           FFLFPGRHWQQDIPSLTAEAAK+HPGISYIITAPLGLHE+LVDVVNDRINHCLSHAAGVA
Sbjct: 135 FFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEKLVDVVNDRINHCLSHAAGVA 194

Query: 255 DECEVCAGTGKCRFN 269
           DECEVCAGTGKCRFN
Sbjct: 195 DECEVCAGTGKCRFN 205

[Close]
BLAST of Lag0030744 vs. ExPASy TrEMBL
Match: A0A6J1K384 (sirohydrochlorin ferrochelatase, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111489668 PE=4 SV=1)

HSP 1 Score: 345.5 bits (885), Expect = 2.0e-91
Identity = 173/197 (87.82%), Postives = 180/197 (91.37%), Query Frame = 0 

Query: 75  PSAGETR-TFTISASQKSPIL--IGRPSRFRNFTLRFCMSVDNGSPNRSRDQIGAGDAVI 134
           PSAGETR T+TI ASQKS IL  IGR S  RNF+ RF MSVDNG  NR    IGAGDAVI
Sbjct: 15  PSAGETRKTYTICASQKSSILIGIGRSSTLRNFSSRFRMSVDNGGSNR----IGAGDAVI 74

Query: 135 IVDHGSRRRESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFASCVEQGAKRIIV 194
           IVDHGSRRRESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFASCVEQGAKRI+V
Sbjct: 75  IVDHGSRRRESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFASCVEQGAKRIVV 134

Query: 195 SPFFLFPGRHWQQDIPSLTAEAAKEHPGISYIITAPLGLHEQLVDVVNDRINHCLSHAAG 254
           SPFFLFPGRHWQQDIPSLTAEAAK+HPGISYIITAPLGLHEQLVDVVNDRI+HCLSHAAG
Sbjct: 135 SPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEQLVDVVNDRISHCLSHAAG 194

Query: 255 VADECEVCAGTGKCRFN 269
           V +ECEVCAGTGKC+FN
Sbjct: 195 VVEECEVCAGTGKCKFN 207

[Close]
BLAST of Lag0030744 vs. ExPASy TrEMBL
Match: A0A6J1BYW9 (sirohydrochlorin ferrochelatase, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111006726 PE=4 SV=1)

HSP 1 Score: 344.0 bits (881), Expect = 5.8e-91
Identity = 166/192 (86.46%), Postives = 174/192 (90.62%), Query Frame = 0 

Query: 76  SAGETRTFTISASQKSPILIGRPSRFRNFTLRFCMSVDNGSPNRSRDQIGAGDAVIIVDH 135
           S  E   + I ASQKS + IGR SRFRNF  RF MSVDNG  NRS  +IG+GDAVIIVDH
Sbjct: 17  SGSEAGRYKICASQKSSVPIGRSSRFRNFASRFRMSVDNGGANRSGARIGSGDAVIIVDH 76

Query: 136 GSRRRESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFASCVEQGAKRIIVSPFF 195
           GSRR+ESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFASCVEQGAK IIVSPFF
Sbjct: 77  GSRRQESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFASCVEQGAKHIIVSPFF 136

Query: 196 LFPGRHWQQDIPSLTAEAAKEHPGISYIITAPLGLHEQLVDVVNDRINHCLSHAAGVADE 255
           LFPGRHWQQDIPSLTAEAAK+HPGISYI+TAPLGLHE+LVDVVNDRINHCLSHAAGVADE
Sbjct: 137 LFPGRHWQQDIPSLTAEAAKDHPGISYIVTAPLGLHEKLVDVVNDRINHCLSHAAGVADE 196

Query: 256 CEVCAGTGKCRF 268
           CEVCAGTGKCRF
Sbjct: 197 CEVCAGTGKCRF 208

[Close]
BLAST of Lag0030744 vs. ExPASy TrEMBL
Match: A0A6J1FHT5 (sirohydrochlorin ferrochelatase, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111445470 PE=4 SV=1)

HSP 1 Score: 344.0 bits (881), Expect = 5.8e-91
Identity = 172/197 (87.31%), Postives = 180/197 (91.37%), Query Frame = 0 

Query: 75  PSAGETR-TFTISASQKSPILI--GRPSRFRNFTLRFCMSVDNGSPNRSRDQIGAGDAVI 134
           PSAGETR T+TI ASQKS ILI  GR S  RNF+ RF MSVDNG      D+IGAGDAVI
Sbjct: 15  PSAGETRKTYTICASQKSSILIGVGRSSTLRNFSSRFRMSVDNG----GADRIGAGDAVI 74

Query: 135 IVDHGSRRRESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFASCVEQGAKRIIV 194
           IVDHGSRRRESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFASCVEQGAKRI+V
Sbjct: 75  IVDHGSRRRESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFASCVEQGAKRIVV 134

Query: 195 SPFFLFPGRHWQQDIPSLTAEAAKEHPGISYIITAPLGLHEQLVDVVNDRINHCLSHAAG 254
           SPFFLFPGRHWQQDIPSLTAEAAK+HPGISYIITAPLGLHEQLVDVVNDRI+HCLSHAAG
Sbjct: 135 SPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEQLVDVVNDRISHCLSHAAG 194

Query: 255 VADECEVCAGTGKCRFN 269
           V +ECEVCAGTGKC+FN
Sbjct: 195 VVEECEVCAGTGKCKFN 207

[Close]
BLAST of Lag0030744 vs. TAIR 10
Match: AT1G50170.1 (sirohydrochlorin ferrochelatase B )

HSP 1 Score: 242.3 bits (617), Expect = 4.5e-64
Identity = 111/146 (76.03%), Postives = 126/146 (86.30%), Query Frame = 0 

Query: 121 RDQIGAGDAVIIVDHGSRRRESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFAS 180
           ++ IG  D +IIVDHGSRRRESNLML EFV MF++KTGYPIVEPAHMELAEPSI+D+F+ 
Sbjct: 75  KNGIGDADGIIIVDHGSRRRESNLMLEEFVKMFKEKTGYPIVEPAHMELAEPSIKDAFSL 134

Query: 181 CVEQGAKRIIVSPFFLFPGRHWQQDIPSLTAEAAKEHPGISYIITAPLGLHEQLVDVVND 240
           CV+QGAKR++VSPFFLFPGRHW  DIPSLTA+AAKE  GISY+ITAPLG H  L+DVVND
Sbjct: 135 CVQQGAKRVVVSPFFLFPGRHWHTDIPSLTADAAKEFSGISYLITAPLGPHNLLLDVVND 194

Query: 241 RINHCLSHAAGVADECEVCAGTGKCR 267
           RI HCLSH  G ADEC VCAGT KC+
Sbjct: 195 RIQHCLSHVEGDADECLVCAGTNKCK 220

The following BLAST results are available for this feature:
BLAST of Lag0030744 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_038890885.11.4e-9491.24sirohydrochlorin ferrochelatase, chloroplastic isoform X1 [Benincasa hispida][more]
KAG7033101.12.2e-9283.08Sirohydrochlorin ferrochelatase, chloroplastic, partial [Cucurbita argyrosperma ... [more]
XP_023551685.11.4e-9187.82sirohydrochlorin ferrochelatase, chloroplastic-like [Cucurbita pepo subsp. pepo][more]
KAA0037556.11.8e-9188.21sirohydrochlorin ferrochelatase [Cucumis melo var. makuwa] >TYK03116.1 sirohydro... [more]
XP_008458790.11.8e-9188.21PREDICTED: sirohydrochlorin ferrochelatase, chloroplastic [Cucumis melo][more]
BLAST of Lag0030744 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q84JH76.4e-6376.03Sirohydrochlorin ferrochelatase, chloroplastic OS=Arabidopsis thaliana OX=3702 G... [more]
P618173.4e-1635.34Sirohydrochlorin ferrochelatase OS=Bacillus megaterium OX=1404 GN=sirB PE=3 SV=1[more]
P618192.1e-1329.08Sirohydrochlorin cobaltochelatase OS=Methanococcus maripaludis (strain S2 / LL) ... [more]
Q583801.3e-1232.61Sirohydrochlorin cobaltochelatase OS=Methanocaldococcus jannaschii (strain ATCC ... [more]
A6UWT52.5e-1128.99Sirohydrochlorin cobaltochelatase OS=Methanococcus aeolicus (strain ATCC BAA-128... [more]
BLAST of Lag0030744 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A5D3BY558.9e-9288.21Sirohydrochlorin ferrochelatase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_... [more]
A0A1S3C8S98.9e-9288.21sirohydrochlorin ferrochelatase, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103... [more]
A0A6J1K3842.0e-9187.82sirohydrochlorin ferrochelatase, chloroplastic-like OS=Cucurbita maxima OX=3661 ... [more]
A0A6J1BYW95.8e-9186.46sirohydrochlorin ferrochelatase, chloroplastic OS=Momordica charantia OX=3673 GN... [more]
A0A6J1FHT55.8e-9187.31sirohydrochlorin ferrochelatase, chloroplastic-like OS=Cucurbita moschata OX=366... [more]
BLAST of Lag0030744 vs. TAIR 10
Match NameE-valueIdentityDescription
AT1G50170.14.5e-6476.03sirohydrochlorin ferrochelatase B [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002762Sirohydrochlorin cobaltochelatase CbiX-likePFAMPF01903CbiXcoord: 135..239
e-value: 4.3E-30
score: 104.1
NoneNo IPR availableGENE3D3.40.50.1400coord: 126..249
e-value: 1.4E-34
score: 120.9
NoneNo IPR availablePANTHERPTHR33542:SF3SIROHYDROCHLORIN FERROCHELATASE, CHLOROPLASTICcoord: 115..265
NoneNo IPR availablePANTHERPTHR33542SIROHYDROCHLORIN FERROCHELATASE, CHLOROPLASTICcoord: 115..265
NoneNo IPR availableCDDcd03416CbiX_SirB_Ncoord: 129..229
e-value: 4.9973E-38
score: 127.303
NoneNo IPR availableSUPERFAMILY53800Chelatasecoord: 128..245

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0030744.1Lag0030744.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006979 response to oxidative stress
biological_process GO:0019354 siroheme biosynthetic process
cellular_component GO:0009507 chloroplast
molecular_function GO:0042802 identical protein binding
molecular_function GO:0051536 iron-sulfur cluster binding
molecular_function GO:0046872 metal ion binding
molecular_function GO:0051266 sirohydrochlorin ferrochelatase activity
molecular_function GO:0016829 lyase activity