Lag0030666 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0030666
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	Cytochrome p450
Location	chr11: 247461 .. 250005 (+)
RNA-Seq Expression	Lag0030666
Synteny	Lag0030666

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTACGTAAGCCCTTCCATATTATATATATATACCATTGATTAAGATATATATACGAATATATATATATATATATATATGATTGGTACGCAAACAAGATTAGATGTTGTCTTTATTTTATTATTATTTTTTTTTATAACATGTGGAGAGGGAGGGAGATTCGAACCACAAATCTTGTGGTTATCAGTACAACTTTATGTCAGTTGAGCTAAGCTCTTGTTGACCATAGATGTCTTTATTTATAAAGTACTCACTCATAATGCTGAACTCTAATAATTGCATTTACATGCATTTTTTTTCTTCTCATTATATCGTCCAAGCTTATATATATATATAGTAATTAAAACTTTGAATGTGATACAGGGAAAATATTTACATATTGGTTGGGGACAGAGCCATTCTTGTACATTGCAGACCCAGAATTTGTGAAGATATTGTCGACAGAAGTGAAGGCCAAGTTCTGGGGAAAGCCATCTGTGTTCAAACGTGACAGAAAATCAATGTTCGGAAATGGTTTGGTAATGGCTGAAGGAGATGAGTGGGTTCGCCAGAGACATGTCATCACTCCTGCATTTAATCCATCCAACTTGAAGGTTTCTAATTCTCTTTTTTCATCCTCTTTTAAATCTTTCATATTATTCTTCAAACCATCTTTTTCTTATCTTCTTTGTCATGTTTTGATTTTTTTTCGAAACATAAATAAATGGACTACTTTTATCAAATGAGTAGACAATTCAATTATTTTAGATTGACTAACAAAAAGAAAAATATTTGGCTGCTTACCCATTAAAATGTTGACAACTAAGAAAGAAAAAGGTAGAAAATGAGAAAAAAAATTTATCTCTCCTACTTGTCAAGATTTTAATAGTGGTATGTAAGGGGATCTTGGAAAAAAAATTCATACTATCTTCATCCATTATTTGTCATGGAAGAAGTACTTAAAAATGATATGGGATCCATGAAATAAAATATTATATCATGAATGCTCATTAAATGACATGTCAAATGTTGAATAGAAGGAAAATACGTACTATATTAGACATTTTTCGGAAGATTCCTACCACCGGTATCGTATATAGTCAAGTATTGTCATAGATTTAAAACATGTCCTATTTTCAAACTATTGTCAATCACACATTCCCATGTGTTTTTCACTTCCCAAGTTGACATTCCGTGTCCATTTCAAACATTTTTCACTCACATCCACAAAATCTTCATTCCTTTTTGAAAACATCATAATTACTAATAACCCAAAATACATGTGTAAACAGGCAATGTCAAGCTTGATGGTGGAGTCCACCACCCACATGCTGGAGAGATGGGCCAACCTCGTACTCTCCGGCCACCCACAAATCGAAGTCGAAAAAGAAATCACTTCCACCGCCGGCGAAATCATCGCCAAAACCAGCTTCGGAATCGACCACAAAAGCGGCCGACAAGTCTTCCAGAAACTCAGGGATCTCCAGATGACACTCTTCAAAACCAACCGCCTCGTCGGAGTCCCGTTCGCCGGAGTTCTCAACGCCGGCAAGGCCCGAGAGGCCAGACGCCTTGGGGAGGAGATCGACGGGCTGTTTCGGGCGGTGATCACGGCGAGGAGAGAGAATCAGGCGGTGCCGCAGCAGCATGATCTTTTGAGTTTGCTTTTGAAAGAGAGCGGCGAAGAGGGGAAGCTGGGGAGGAGGCTGAGCACGACGGAGCTGATCGACGAGTGTAAGACGTTTTTCTTTGGTGGGCATGAAACGACGGCGTTGGCTCTGACGTGGACACTGCTGCTTTTGGGCATTCATACGGAGTGGCAAACGCAGCTGAGAGAGGAGATCAAACAAGTGCTTGGTGATAAACACACTGACTTTGATTTCACTAAGCTTTCTCAACTCAGAAAGGTAAATACCAGCATACACTTTATGCTTTCATTTGTTGACATATTATCTCTATACACATCATCATTTATTGACAATCATGGGAAAAAGAAAAAAAAAATCTCATTCTCAATTTTTCTCCGTCTTAGTTGTCAACATTTCAATGATAGTTTGTATGAAGATATATATCCACGTACTGTTATGCAGTCAAATATTGAATGTGACGAGAGAATTTGAAAAAATGCAGATGGGATGGGTAATGAGCGAGGTTCTACGTCTATACCCATCAGCACCAAACGTACAAAGACAAGCAAGAAGAGACATTACAATAAACGGAGTGACAATCCCGAATGGCACCAACATGTGGATCGACGTGGTGGGCATGCATCACGACCAAGCCCTTTGGGGAGACCAGGTCAACGAGTTCCACCCTGAGAGATTCCAAAACGACGCCATTGCTGGCGGCTGCAGCCACAAGATGGGCTATTTGCCATTTGGGTTTGGAGGAAGAATGTGTGTTGGAAGGCACTTGACGTTTATGGAGTATAAGATTGTGTTGACCTTGATTTTGTCTAGGTTTTCCTTCTCTTTGTCTCCTGATTACTGCCATTCGCCTTCCATTATGCTCTCCCTCCGACCTGCCCATGGCTTGCCCCTCATTTTTCAACTACTCGAGGATTAG

mRNA sequence

ATGGTACACCCAGAATTTGTGAAGATATTGTCGACAGAAGTGAAGGCCAAGTTCTGGGGAAAGCCATCTGTGTTCAAACGTGACAGAAAATCAATGTTCGGAAATGGTTTGGTAATGGCTGAAGGAGATGAGTGGGTTCGCCAGAGACATGTCATCACTCCTGCATTTAATCCATCCAACTTGAAGGCAATGTCAAGCTTGATGGTGGAGTCCACCACCCACATGCTGGAGAGATGGGCCAACCTCGTACTCTCCGGCCACCCACAAATCGAAGTCGAAAAAGAAATCACTTCCACCGCCGGCGAAATCATCGCCAAAACCAGCTTCGGAATCGACCACAAAAGCGGCCGACAAGTCTTCCAGAAACTCAGGGATCTCCAGATGACACTCTTCAAAACCAACCGCCTCGTCGGAGTCCCGTTCGCCGGAGTTCTCAACGCCGGCAAGGCCCGAGAGGCCAGACGCCTTGGGGAGGAGATCGACGGGCTGTTTCGGGCGGTGATCACGGCGAGGAGAGAGAATCAGGCGGTGCCGCAGCAGCATGATCTTTTGAGTTTGCTTTTGAAAGAGAGCGGCGAAGAGGGGAAGCTGGGGAGGAGGCTGAGCACGACGGAGCTGATCGACGAGTGTAAGACGTTTTTCTTTGGTGGGCATGAAACGACGGCGTTGGCTCTGACGTGGACACTGCTGCTTTTGGGCATTCATACGGAGTGGCAAACGCAGCTGAGAGAGGAGATCAAACAAGTGCTTGGTGATAAACACACTGACTTTGATTTCACTAAGCTTTCTCAACTCAGAAAGATGGGATGGGTAATGAGCGAGGTTCTACGTCTATACCCATCAGCACCAAACGTACAAAGACAAGCAAGAAGAGACATTACAATAAACGGAGTGACAATCCCGAATGGCACCAACATGTGGATCGACGTGGTGGGCATGCATCACGACCAAGCCCTTTGGGGAGACCAGGTCAACGAGTTCCACCCTGAGAGATTCCAAAACGACGCCATTGCTGGCGGCTGCAGCCACAAGATGGGCTATTTGCCATTTGGGTTTGGAGGAAGAATGTGTGTTGGAAGGCACTTGACGTTTATGGAGTATAAGATTGTGTTGACCTTGATTTTGTCTAGGTTTTCCTTCTCTTTGTCTCCTGATTACTGCCATTCGCCTTCCATTATGCTCTCCCTCCGACCTGCCCATGGCTTGCCCCTCATTTTTCAACTACTCGAGGATTAG

Coding sequence (CDS)

ATGGTACACCCAGAATTTGTGAAGATATTGTCGACAGAAGTGAAGGCCAAGTTCTGGGGAAAGCCATCTGTGTTCAAACGTGACAGAAAATCAATGTTCGGAAATGGTTTGGTAATGGCTGAAGGAGATGAGTGGGTTCGCCAGAGACATGTCATCACTCCTGCATTTAATCCATCCAACTTGAAGGCAATGTCAAGCTTGATGGTGGAGTCCACCACCCACATGCTGGAGAGATGGGCCAACCTCGTACTCTCCGGCCACCCACAAATCGAAGTCGAAAAAGAAATCACTTCCACCGCCGGCGAAATCATCGCCAAAACCAGCTTCGGAATCGACCACAAAAGCGGCCGACAAGTCTTCCAGAAACTCAGGGATCTCCAGATGACACTCTTCAAAACCAACCGCCTCGTCGGAGTCCCGTTCGCCGGAGTTCTCAACGCCGGCAAGGCCCGAGAGGCCAGACGCCTTGGGGAGGAGATCGACGGGCTGTTTCGGGCGGTGATCACGGCGAGGAGAGAGAATCAGGCGGTGCCGCAGCAGCATGATCTTTTGAGTTTGCTTTTGAAAGAGAGCGGCGAAGAGGGGAAGCTGGGGAGGAGGCTGAGCACGACGGAGCTGATCGACGAGTGTAAGACGTTTTTCTTTGGTGGGCATGAAACGACGGCGTTGGCTCTGACGTGGACACTGCTGCTTTTGGGCATTCATACGGAGTGGCAAACGCAGCTGAGAGAGGAGATCAAACAAGTGCTTGGTGATAAACACACTGACTTTGATTTCACTAAGCTTTCTCAACTCAGAAAGATGGGATGGGTAATGAGCGAGGTTCTACGTCTATACCCATCAGCACCAAACGTACAAAGACAAGCAAGAAGAGACATTACAATAAACGGAGTGACAATCCCGAATGGCACCAACATGTGGATCGACGTGGTGGGCATGCATCACGACCAAGCCCTTTGGGGAGACCAGGTCAACGAGTTCCACCCTGAGAGATTCCAAAACGACGCCATTGCTGGCGGCTGCAGCCACAAGATGGGCTATTTGCCATTTGGGTTTGGAGGAAGAATGTGTGTTGGAAGGCACTTGACGTTTATGGAGTATAAGATTGTGTTGACCTTGATTTTGTCTAGGTTTTCCTTCTCTTTGTCTCCTGATTACTGCCATTCGCCTTCCATTATGCTCTCCCTCCGACCTGCCCATGGCTTGCCCCTCATTTTTCAACTACTCGAGGATTAG

Protein sequence

MVHPEFVKILSTEVKAKFWGKPSVFKRDRKSMFGNGLVMAEGDEWVRQRHVITPAFNPSNLKAMSSLMVESTTHMLERWANLVLSGHPQIEVEKEITSTAGEIIAKTSFGIDHKSGRQVFQKLRDLQMTLFKTNRLVGVPFAGVLNAGKAREARRLGEEIDGLFRAVITARRENQAVPQQHDLLSLLLKESGEEGKLGRRLSTTELIDECKTFFFGGHETTALALTWTLLLLGIHTEWQTQLREEIKQVLGDKHTDFDFTKLSQLRKMGWVMSEVLRLYPSAPNVQRQARRDITINGVTIPNGTNMWIDVVGMHHDQALWGDQVNEFHPERFQNDAIAGGCSHKMGYLPFGFGGRMCVGRHLTFMEYKIVLTLILSRFSFSLSPDYCHSPSIMLSLRPAHGLPLIFQLLED

Homology

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BLAST of Lag0030666 vs. NCBI nr
Match: XP_022958036.1 (cytokinin hydroxylase-like [Cucurbita moschata])

HSP 1 Score: 753.8 bits (1945), Expect = 7.6e-214
Identity = 369/413 (89.35%), Postives = 392/413 (94.92%), Query Frame = 0


Query: 1   MVHPEFVKILSTEVKAKFWGKPSVFKRDRKSMFGNGLVMAEGDEWVRQRHVITPAFNPSN 60
           +  PEFVK+LS EV+AK+WGKPSVFKRDRKSMFGNGLVMAEGDEWVRQRHVITPAFNPSN
Sbjct: 104 VAEPEFVKMLSREVQAKYWGKPSVFKRDRKSMFGNGLVMAEGDEWVRQRHVITPAFNPSN 163

Query: 61  LKAMSSLMVESTTHMLERWANLVLSGHPQIEVEKEITSTAGEIIAKTSFGIDHKSGRQVF 120
           LKAM+SLMVESTT ML+RWA+LVLSGHPQIEVE EITSTAGEIIAKTSFGIDH SGRQVF
Sbjct: 164 LKAMASLMVESTTQMLDRWAHLVLSGHPQIEVENEITSTAGEIIAKTSFGIDHISGRQVF 223

Query: 121 QKLRDLQMTLFKTNRLVGVPFAGVLNAGKAREARRLGEEIDGLFRAVITARRENQA---V 180
            KLR+LQMTLFKTNRLVGVPFAG+LNAGKAREA+RLGEEID LFR VITARRENQA   V
Sbjct: 224 HKLRNLQMTLFKTNRLVGVPFAGILNAGKAREAKRLGEEIDELFREVITARRENQAAAEV 283

Query: 181 PQQHDLLSLLLKESGEEGKLGRRLSTTELIDECKTFFFGGHETTALALTWTLLLLGIHTE 240
            QQ+DLLSLLLKESG EGKLGR LSTTELIDECKTFFFGGHETTALALTWTLLLLGIHTE
Sbjct: 284 VQQNDLLSLLLKESGGEGKLGRWLSTTELIDECKTFFFGGHETTALALTWTLLLLGIHTE 343

Query: 241 WQTQLREEIKQVLGDKHTDFDFTKLSQLRKMGWVMSEVLRLYPSAPNVQRQARRDITING 300
           WQTQLR+EIK+VLGDK +DFDFTKLSQL+KMGWVMSEVLRLYPSAPNVQRQARRDIT+NG
Sbjct: 344 WQTQLRDEIKEVLGDKESDFDFTKLSQLKKMGWVMSEVLRLYPSAPNVQRQARRDITVNG 403

Query: 301 VTIPNGTNMWIDVVGMHHDQALWGDQVNEFHPERFQNDAIAGGCSHKMGYLPFGFGGRMC 360
           +TIPNGTNMWIDVV MHHD+ALWG+QVNEF PERF++D +AGGCSHKMGYLPFGFGGRMC
Sbjct: 404 LTIPNGTNMWIDVVAMHHDEALWGEQVNEFRPERFEHDGVAGGCSHKMGYLPFGFGGRMC 463

Query: 361 VGRHLTFMEYKIVLTLILSRFSFSLSPDYCHSPSIMLSLRPAHGLPLIFQLLE 411
           VGRHLTFMEYKIVLTLIL RFSF+LSPDYCHSP IMLSLRPAHGLPLIFQLLE
Sbjct: 464 VGRHLTFMEYKIVLTLILCRFSFTLSPDYCHSPCIMLSLRPAHGLPLIFQLLE 516

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BLAST of Lag0030666 vs. NCBI nr
Match: KAG7035636.1 (Cytochrome P450, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 753.8 bits (1945), Expect = 7.6e-214
Identity = 370/410 (90.24%), Postives = 391/410 (95.37%), Query Frame = 0


Query: 4   PEFVKILSTEVKAKFWGKPSVFKRDRKSMFGNGLVMAEGDEWVRQRHVITPAFNPSNLKA 63
           PEFVK+LS EV+AK+WGKPSVFKRDRKSMFGNGLVMAEGDEWVRQRHVITPAFNPSNLKA
Sbjct: 79  PEFVKMLSREVQAKYWGKPSVFKRDRKSMFGNGLVMAEGDEWVRQRHVITPAFNPSNLKA 138

Query: 64  MSSLMVESTTHMLERWANLVLSGHPQIEVEKEITSTAGEIIAKTSFGIDHKSGRQVFQKL 123
           M+SLMVESTT ML+RWA+LVLSGHPQIEVE EITSTAGEIIAKTSFGIDH SGRQVF KL
Sbjct: 139 MASLMVESTTQMLDRWAHLVLSGHPQIEVENEITSTAGEIIAKTSFGIDHISGRQVFHKL 198

Query: 124 RDLQMTLFKTNRLVGVPFAGVLNAGKAREARRLGEEIDGLFRAVITARRENQA---VPQQ 183
           R+LQMTLFKTNRLVGVPFAGVLNAGKAREA+RLGEEID LFR VITARRENQA   V QQ
Sbjct: 199 RNLQMTLFKTNRLVGVPFAGVLNAGKAREAKRLGEEIDELFREVITARRENQAAAEVVQQ 258

Query: 184 HDLLSLLLKESGEEGKLGRRLSTTELIDECKTFFFGGHETTALALTWTLLLLGIHTEWQT 243
           +DLLSLLLKESG EGKLGR LSTTELIDECKTFFFGGHETTALALTWTLLLLGIHTEWQT
Sbjct: 259 NDLLSLLLKESGGEGKLGRWLSTTELIDECKTFFFGGHETTALALTWTLLLLGIHTEWQT 318

Query: 244 QLREEIKQVLGDKHTDFDFTKLSQLRKMGWVMSEVLRLYPSAPNVQRQARRDITINGVTI 303
           QLR+EIK+VLGDK +DFDFTKLSQL+KMGWVMSEVLRLYPSAPNVQRQARRDIT+NG+TI
Sbjct: 319 QLRDEIKEVLGDKESDFDFTKLSQLKKMGWVMSEVLRLYPSAPNVQRQARRDITVNGLTI 378

Query: 304 PNGTNMWIDVVGMHHDQALWGDQVNEFHPERFQNDAIAGGCSHKMGYLPFGFGGRMCVGR 363
           PNGTNMWIDVV MHHD+ALWG+QVNEF PERF++D +AGGCSHKMGYLPFGFGGRMCVGR
Sbjct: 379 PNGTNMWIDVVAMHHDEALWGEQVNEFRPERFEHDGVAGGCSHKMGYLPFGFGGRMCVGR 438

Query: 364 HLTFMEYKIVLTLILSRFSFSLSPDYCHSPSIMLSLRPAHGLPLIFQLLE 411
           HLTFMEYKIVLTLIL RFSF+LSPDYCHSP IMLSLRPAHGLPLIFQLLE
Sbjct: 439 HLTFMEYKIVLTLILCRFSFTLSPDYCHSPCIMLSLRPAHGLPLIFQLLE 488

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BLAST of Lag0030666 vs. NCBI nr
Match: XP_023533013.1 (cytokinin hydroxylase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 747.7 bits (1929), Expect = 5.5e-212
Identity = 367/417 (88.01%), Postives = 391/417 (93.76%), Query Frame = 0


Query: 1   MVHPEFVKILSTEVKAKFWGKPSVFKRDRKSMFGNGLVMAEGDEWVRQRHVITPAFNPSN 60
           +  PEFV++LS EV+AK+WGKPSVFKRDRKSMFGNGLVMAEGDEWVRQRHVITPAFNPSN
Sbjct: 93  IAEPEFVRMLSREVQAKYWGKPSVFKRDRKSMFGNGLVMAEGDEWVRQRHVITPAFNPSN 152

Query: 61  LKAMSSLMVESTTHMLERWANLVLSGHPQIEVEKEITSTAGEIIAKTSFGIDHKSGRQVF 120
           LKAM+SLMVESTT ML+RWA+LVLSGHPQIEVE EITSTAGEIIAKTSFGIDH SGRQVF
Sbjct: 153 LKAMASLMVESTTQMLDRWAHLVLSGHPQIEVENEITSTAGEIIAKTSFGIDHISGRQVF 212

Query: 121 QKLRDLQMTLFKTNRLVGVPFAGVLNAGKAREARRLGEEIDGLFRAVITARRENQA---- 180
            KLR+LQMTLFKTNRLVGVPFAG+L AGKAREA+RLGEEID LFR VITARRENQA    
Sbjct: 213 HKLRNLQMTLFKTNRLVGVPFAGILIAGKAREAKRLGEEIDELFREVITARRENQAAAAA 272

Query: 181 ---VPQQHDLLSLLLKESGEEGKLGRRLSTTELIDECKTFFFGGHETTALALTWTLLLLG 240
              V QQ+DLLSLLLKESG EGKLGR LSTTELIDECKTFFFGGHETTALALTWTLLLLG
Sbjct: 273 AAEVVQQNDLLSLLLKESGGEGKLGRWLSTTELIDECKTFFFGGHETTALALTWTLLLLG 332

Query: 241 IHTEWQTQLREEIKQVLGDKHTDFDFTKLSQLRKMGWVMSEVLRLYPSAPNVQRQARRDI 300
           IHTEWQTQLR+EIK+VLGDK +DFDFTKLSQL+KMGWVMSEVLRLYPSAPNVQRQARRDI
Sbjct: 333 IHTEWQTQLRDEIKEVLGDKESDFDFTKLSQLKKMGWVMSEVLRLYPSAPNVQRQARRDI 392

Query: 301 TINGVTIPNGTNMWIDVVGMHHDQALWGDQVNEFHPERFQNDAIAGGCSHKMGYLPFGFG 360
           T+NG+TIPNGTNMWIDVV MHHD+ALWG+QVNEF PERF++D +AGGCSHKMGYLPFGFG
Sbjct: 393 TVNGLTIPNGTNMWIDVVAMHHDEALWGEQVNEFRPERFEHDGVAGGCSHKMGYLPFGFG 452

Query: 361 GRMCVGRHLTFMEYKIVLTLILSRFSFSLSPDYCHSPSIMLSLRPAHGLPLIFQLLE 411
           GRMCVGRHLTFMEYKIVLTLIL RFSF+LSPDYCHSP IMLSLRPAHGLPLIFQLLE
Sbjct: 453 GRMCVGRHLTFMEYKIVLTLILCRFSFTLSPDYCHSPCIMLSLRPAHGLPLIFQLLE 509

[Close]

BLAST of Lag0030666 vs. NCBI nr
Match: XP_022995689.1 (cytokinin hydroxylase-like [Cucurbita maxima])

HSP 1 Score: 740.3 bits (1910), Expect = 8.7e-210
Identity = 363/417 (87.05%), Postives = 389/417 (93.29%), Query Frame = 0


Query: 1   MVHPEFVKILSTEVKAKFWGKPSVFKRDRKSMFGNGLVMAEGDEWVRQRHVITPAFNPSN 60
           +  PEFVK+LS EV+AK+WGKPSVFKRDRKSMFGNGLVMAEGDEWVRQRHVITPAFNPSN
Sbjct: 104 LADPEFVKMLSREVQAKYWGKPSVFKRDRKSMFGNGLVMAEGDEWVRQRHVITPAFNPSN 163

Query: 61  LKAMSSLMVESTTHMLERWANLVLSGHPQIEVEKEITSTAGEIIAKTSFGIDHKSGRQVF 120
           LKAM+SLMVESTT ML+RWA+LVLSGHPQIEVE EITSTAGEIIAKTSFGIDH SGRQVF
Sbjct: 164 LKAMASLMVESTTQMLDRWAHLVLSGHPQIEVENEITSTAGEIIAKTSFGIDHISGRQVF 223

Query: 121 QKLRDLQMTLFKTNRLVGVPFAGVLNAGKAREARRLGEEIDGLFRAVITARRENQA---- 180
            KLR+LQMTLFKTNRLVGVPFAG+LNAGKAREA+RLGEEID LFR VITARRENQ+    
Sbjct: 224 HKLRNLQMTLFKTNRLVGVPFAGILNAGKAREAKRLGEEIDELFREVITARRENQSATAA 283

Query: 181 --VPQQHDLLSLLLKESGEEGKLGRRLSTTELIDECKTFFFGGHETTALALTWTLLLLGI 240
             V  Q+DLLSLLLKESG EGKL R LST ELIDECKTFFFGGHETTALALTWTLLLLGI
Sbjct: 284 AEVVLQNDLLSLLLKESGGEGKLRRWLSTAELIDECKTFFFGGHETTALALTWTLLLLGI 343

Query: 241 HTEWQTQLREEIKQVLGDKHTDFDFTKLSQLRKMGWVMSEVLRLYPSAPNVQRQARRDIT 300
           HTEWQ QLR+EIK+VLGDK +DFDFTKLSQL+KMGWVMSEVLRLYPSAPNVQRQARRDIT
Sbjct: 344 HTEWQIQLRDEIKEVLGDKESDFDFTKLSQLKKMGWVMSEVLRLYPSAPNVQRQARRDIT 403

Query: 301 INGVTIPNGTNMWIDVVGMHHDQALWGDQVNEFHPERFQNDAIAGGCSHKMGYLPFGFGG 360
           +NG+TIPNGTNMWIDVV MHHD+ALWG+QVNEF PERF++D +AGGCSHKMGYLPFGFGG
Sbjct: 404 VNGLTIPNGTNMWIDVVAMHHDEALWGEQVNEFRPERFEHDEVAGGCSHKMGYLPFGFGG 463

Query: 361 RMCVGRHLTFMEYKIVLTLILSRFSFSLSPDYCHSPSIMLSLRPAHGLPLIFQLLED 412
           RMCVGRHLTFMEYKIVLTLIL RFSF+LSPDYCHSP IMLSLRPAHGLPLIF+LLE+
Sbjct: 464 RMCVGRHLTFMEYKIVLTLILCRFSFTLSPDYCHSPCIMLSLRPAHGLPLIFRLLEN 520

[Close]

BLAST of Lag0030666 vs. NCBI nr
Match: KAG6605729.1 (Cytochrome P450 72A11, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 704.1 bits (1816), Expect = 6.9e-199
Identity = 351/413 (84.99%), Postives = 373/413 (90.31%), Query Frame = 0


Query: 1   MVHPEFVKILSTEVKAKFWGKPSVFKRDRKSMFGNGLVMAEGDEWVRQRHVITPAFNPSN 60
           +  PEFVK+LS EV+AK+WGKPSVFKRDRKSMFGNGLVMAEGDEW               
Sbjct: 104 VAEPEFVKMLSREVQAKYWGKPSVFKRDRKSMFGNGLVMAEGDEW--------------- 163

Query: 61  LKAMSSLMVESTTHMLERWANLVLSGHPQIEVEKEITSTAGEIIAKTSFGIDHKSGRQVF 120
             AM+SLMVESTT ML+RWA LVLSGHPQIEVE EITSTAGEIIAKTSFGIDH SGRQVF
Sbjct: 164 --AMASLMVESTTQMLDRWAQLVLSGHPQIEVENEITSTAGEIIAKTSFGIDHISGRQVF 223

Query: 121 QKLRDLQMTLFKTNRLVGVPFAGVLNAGKAREARRLGEEIDGLFRAVITARRENQA---V 180
            KLR+LQMTLFKTNRLVGVPFAGVLNAGKAREA+RLGEEID LFR VITARRENQA   V
Sbjct: 224 HKLRNLQMTLFKTNRLVGVPFAGVLNAGKAREAKRLGEEIDELFREVITARRENQAAAEV 283

Query: 181 PQQHDLLSLLLKESGEEGKLGRRLSTTELIDECKTFFFGGHETTALALTWTLLLLGIHTE 240
            QQ+DLLSLLLKESG EGKLGR LSTTELIDECKTFFFGGHETTALALTWTLLLLGIHTE
Sbjct: 284 VQQNDLLSLLLKESGGEGKLGRWLSTTELIDECKTFFFGGHETTALALTWTLLLLGIHTE 343

Query: 241 WQTQLREEIKQVLGDKHTDFDFTKLSQLRKMGWVMSEVLRLYPSAPNVQRQARRDITING 300
           WQ QLR+EIK+VLGDK +DFDFTKLSQL+KMGWVMSEVLRLYPSAPNVQRQARRDIT+NG
Sbjct: 344 WQIQLRDEIKEVLGDKESDFDFTKLSQLKKMGWVMSEVLRLYPSAPNVQRQARRDITVNG 403

Query: 301 VTIPNGTNMWIDVVGMHHDQALWGDQVNEFHPERFQNDAIAGGCSHKMGYLPFGFGGRMC 360
           +TIPNGTNMWIDVV MHHD+ALWG+QVNEF PERF++D +AGGCSHKMGYLPFGFGGRMC
Sbjct: 404 LTIPNGTNMWIDVVAMHHDEALWGEQVNEFRPERFEHDGVAGGCSHKMGYLPFGFGGRMC 463

Query: 361 VGRHLTFMEYKIVLTLILSRFSFSLSPDYCHSPSIMLSLRPAHGLPLIFQLLE 411
           VGRHLTFMEYKIVLTLIL RFSF+LSPDYCHSP IMLSLRPAHGLPLIF+LLE
Sbjct: 464 VGRHLTFMEYKIVLTLILCRFSFTLSPDYCHSPCIMLSLRPAHGLPLIFRLLE 499

[Close]

BLAST of Lag0030666 vs. ExPASy Swiss-Prot
Match: Q2QYH7 (Cytochrome P450 714C2 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP714C2 PE=2 SV=1)

HSP 1 Score: 275.8 bits (704), Expect = 8.0e-73
Identity = 147/408 (36.03%), Postives = 225/408 (55.15%), Query Frame = 0


Query: 4   PEFVKILSTEVKAKFWGKPSVFKRDRKSMFGNGLVMAEGDEWVRQRHVITPAFNPSNLKA 63
           P  VK L+   K+   GKP   +++R ++ G G++ + GD WV QR VI P      +K 
Sbjct: 120 PNMVKELA-NCKSLDLGKPCYLQKERGALLGMGILTSNGDLWVHQRKVIAPELFMERVKG 179

Query: 64  MSSLMVESTTHMLERWANLV--LSGHPQIEVEKEITSTAGEIIAKTSFGIDHKSGRQVFQ 123
           M +LM+E+   ML  W N V    G  +I V++ + + + ++I++  FG     G+++F 
Sbjct: 180 MVNLMMEAAMSMLNSWKNEVEDRGGSAEIVVDEFLRTFSADVISRACFGSSFSEGKEIFI 239

Query: 124 KLRDLQMTLFKTNRLVGVPFAGVLNAGKAREARRLGEEIDGLFRAVITARRENQAVPQQH 183
           K+R LQ  + K + L+GVP +  L     R    L   I  L   +      + +     
Sbjct: 240 KIRQLQKAMAKQSMLIGVPGSRYLPTRSNRGIWNLDSSIRTLILNISKKYEHDSSTSVNK 299

Query: 184 DLLSLLLKESGEEGKLGRRLSTTELIDECKTFFFGGHETTALALTWTLLLLGIHTEWQTQ 243
           DLL  +++ S ++G          ++D CK  +F GHETT+    W L+LL  H EWQ++
Sbjct: 300 DLLHSIIQGS-KDGPFASCTPEDFIVDNCKNIYFAGHETTSTTAAWCLMLLASHHEWQSR 359

Query: 244 LREEIKQVLGDKHTDFDFTKLSQLRKMGWVMSEVLRLYPSAPNVQRQARRDITINGVTIP 303
            R E   +   +  DFD   L +L+K+  V+ E LRLYP A  V R+A  D+ + G+ IP
Sbjct: 360 ARVESLDICQGRPLDFDI--LRKLKKLTMVIQETLRLYPPASFVAREALNDMKLGGIDIP 419

Query: 304 NGTNMWIDVVGMHHDQALWGDQVNEFHPERFQNDAIAGGCSHKMGYLPFGFGGRMCVGRH 363
            GTN+WI +   H D ++WG   ++F P+RF N  IAG C     Y+PFG G R C G++
Sbjct: 420 KGTNIWIPIAMAHRDPSVWGPSADKFDPDRFAN-GIAGACKPPHMYMPFGVGVRTCAGQN 479

Query: 364 LTFMEYKIVLTLILSRFSFSLSPDYCHSPSIMLSLRPAHGLPLIFQLL 410
           L  +E K+VL+L+LS+F F LSP+Y H P+  L++ P  G+PLIF+ L
Sbjct: 480 LAMVELKVVLSLLLSKFEFKLSPNYVHCPAFRLTIEPGKGVPLIFREL 522

[Close]

BLAST of Lag0030666 vs. ExPASy Swiss-Prot
Match: Q9FF18 (Cytokinin hydroxylase OS=Arabidopsis thaliana OX=3702 GN=CYP735A1 PE=1 SV=1)

HSP 1 Score: 265.4 bits (677), Expect = 1.1e-69
Identity = 142/395 (35.95%), Postives = 227/395 (57.47%), Query Frame = 0


Query: 20  GKPSVFKRDRKSMFGNGLVMAEGDEWVRQRHVITPAFNPSNLKAMSSLMVESTTHMLERW 79
           G+  + ++  K+  G GL+MA G +W  QRH+  PAF    LK  +  MVE T+ ++ER 
Sbjct: 127 GRSWLQQQGTKNFIGRGLLMANGQDWHHQRHLAAPAFTGERLKGYARHMVECTSKLVERL 186

Query: 80  ANLVLSGHPQIEVEKEITSTAGEIIAKTSFGIDHKSGRQVFQKLRDLQMTLFKTNRLVGV 139
              V  G  ++E+ +E+     +II++T FG   + G+++F  L  LQ    +  R +  
Sbjct: 187 RKEVGEGANEVEIGEEMHKLTADIISRTKFGSSFEKGKELFNHLTVLQRRCAQATRHLCF 246

Query: 140 PFAGVLNAGKAREARRLGEEIDGLFRAVITARRE----NQAVPQQHDLLSLLLKESGEEG 199
           P +  L +   RE + L +E++ L   +I +RR+     ++     DLL LLL E   + 
Sbjct: 247 PGSRFLPSKYNREIKSLKKEVERLLIEIIQSRRDCAEMGRSSTHGDDLLGLLLNEMDIDK 306

Query: 200 KLGRRLSTTELI-DECKTFFFGGHETTALALTWTLLLLGIHTEWQTQLREEIKQVLGDKH 259
                 +  +LI DECKTFFF GHETTAL LTWT +LL  +  WQ ++REE+++V G   
Sbjct: 307 NNNNNNNNLQLIMDECKTFFFAGHETTALLLTWTTMLLADNPTWQEKVREEVREVFGRNG 366

Query: 260 TDFDFTKLSQLRKMGWVMSEVLRLYPSAPNVQRQARRDITINGVTIPNGTNMWIDVVGMH 319
                 +LS+L  +  V++E LRLYP A  + R A  D+ +  +TIP G ++WI V+ +H
Sbjct: 367 LP-SVDQLSKLTSLSKVINESLRLYPPATLLPRMAFEDLKLGDLTIPKGLSIWIPVLAIH 426

Query: 320 HDQALWGDQVNEFHPERFQNDAIAGGCSHKMGYLPFGFGGRMCVGRHLTFMEYKIVLTLI 379
           H + LWG   N+F+PERF     A G      ++PF  G R C+G+    ME KI+L  +
Sbjct: 427 HSEELWGKDANQFNPERFGGRPFASG----RHFIPFAAGPRNCIGQQFALMEAKIILATL 486

Query: 380 LSRFSFSLSPDYCHSPSIMLSLRPAHGLPLIFQLL 410
           +S+F+F++S +Y H+P ++L+++P +G+ +I + L
Sbjct: 487 ISKFNFTISKNYRHAPIVVLTIKPKYGVQVILKPL 516

[Close]

BLAST of Lag0030666 vs. ExPASy Swiss-Prot
Match: Q9ZW95 (Cytokinin hydroxylase OS=Arabidopsis thaliana OX=3702 GN=CYP735A2 PE=1 SV=1)

HSP 1 Score: 258.5 bits (659), Expect = 1.3e-67
Identity = 141/395 (35.70%), Postives = 221/395 (55.95%), Query Frame = 0


Query: 20  GKPSVFKRDRKSMFGNGLVMAEGDEWVRQRHVITPAFNPSNLKAMSSLMVESTTHMLERW 79
           GK  + ++  K   G GL+MA G+ W  QRH+  PAF    LK  +  MVE T  M ER 
Sbjct: 128 GKSWLQQQGTKGFIGRGLLMANGEAWHHQRHMAAPAFTRDRLKGYAKHMVECTKMMAERL 187

Query: 80  ANLVLSGHPQIEVEKEITSTAGEIIAKTSFGIDHKSGRQVFQKLRDLQMTLFKTNRLVGV 139
              V     ++E+ +E+     +II++T FG     G+++F  L  LQ    +  R +  
Sbjct: 188 RKEV---GEEVEIGEEMRRLTADIISRTEFGSSCDKGKELFSLLTVLQRLCAQATRHLCF 247

Query: 140 PFAGVLNAGKAREARRLGEEIDGLFRAVITARRENQAVPQQ----HDLLSLLLKESGEEG 199
           P +  L +   RE + L  E++ L   +I +R+++  + +      DLL LLL +     
Sbjct: 248 PGSRFLPSKYNREIKSLKTEVERLLMEIIDSRKDSVEIGRSSSYGDDLLGLLLNQMDSN- 307

Query: 200 KLGRRLSTTELIDECKTFFFGGHETTALALTWTLLLLGIHTEWQTQLREEIKQVLGDKHT 259
                L+   ++DECKTFFF GHETT+L LTWTL+LL  +  WQ  +R+E++QV G    
Sbjct: 308 --KNNLNVQMIMDECKTFFFTGHETTSLLLTWTLMLLAHNPTWQDNVRDEVRQVCGQDGV 367

Query: 260 DFDFTKLSQLRKMGWVMSEVLRLYPSAPNVQRQARRDITINGVTIPNGTNMWIDVVGMHH 319
                +LS L  +  V++E LRLYP A  + R A  DI +  + IP G ++WI V+ +HH
Sbjct: 368 P-SVEQLSSLTSLNKVINESLRLYPPATLLPRMAFEDIKLGDLIIPKGLSIWIPVLAIHH 427

Query: 320 DQALWGDQVNEFHPERFQNDAIAGGCSHKMGYLPFGFGGRMCVGRHLTFMEYKIVLTLIL 379
              LWG+  NEF+PERF   + A        ++PF  G R C+G+    ME KI+L +++
Sbjct: 428 SNELWGEDANEFNPERFTTRSFAS----SRHFMPFAAGPRNCIGQTFAMMEAKIILAMLV 487

Query: 380 SRFSFSLSPDYCHSPSIMLSLRPAHGLPLIFQLLE 411
           S+FSF++S +Y H+P ++L+++P +G+ L+ + L+
Sbjct: 488 SKFSFAISENYRHAPIVVLTIKPKYGVQLVLKPLD 511

[Close]

BLAST of Lag0030666 vs. ExPASy Swiss-Prot
Match: Q9ASR3 (Cytochrome P450 709B1 OS=Arabidopsis thaliana OX=3702 GN=CYP709B1 PE=2 SV=1)

HSP 1 Score: 253.1 bits (645), Expect = 5.5e-66
Identity = 149/414 (35.99%), Postives = 231/414 (55.80%), Query Frame = 0


Query: 4   PEFVKILSTEVKAKFWGKPSVFKRDRKSMFGNGLVMAEGDEWVRQRHVITPAFNPSNLKA 63
           PE  K + +  K  F+ K        K +   GLV  EG +WVR R ++ PAF+   LK 
Sbjct: 111 PELAKTMLSN-KLGFFVKSKARPEAVKLVGSKGLVFIEGADWVRHRRILNPAFSIDRLKI 170

Query: 64  MSSLMVESTTHMLERW---ANLVLSGHPQI--EVEKEITSTAGEIIAKTSFGIDHKSGRQ 123
           M+++MV+ T  MLE W   +    + HP+I  E+ +E      +IIA ++FG  +  G +
Sbjct: 171 MTTVMVDCTLKMLEEWRKESTKEETEHPKIKKEMNEEFQRLTADIIATSAFGSSYVEGIE 230

Query: 124 VFQKLRDLQMTLFKTNRLVGVPFAGVLNAGKAREARRLGEEIDGLFRAVITARRENQAVP 183
           VF+   +L+     +   V +P    L         +L  ++D   + +I++R ++++  
Sbjct: 231 VFRSQMELKRCYTTSLNQVSIPGTQYLPTPSNIRVWKLERKMDNSIKRIISSRLQSKS-D 290

Query: 184 QQHDLLSLLLKESGEEGKLGRRLSTTELIDECKTFFFGGHETTALALTWTLLLLGIHTEW 243
              DLL +LLK    EGK  R++S  E+I EC+TFFFGGHETT+  L WT +LL +H +W
Sbjct: 291 YGDDLLGILLKAYNTEGK-ERKMSIEEIIHECRTFFFGGHETTSNLLAWTTMLLSLHQDW 350

Query: 244 QTQLREEIKQVLGDKHTDFDFTKLSQLRKMGWVMSEVLRLYPSAPNVQRQARRDITINGV 303
           Q +LREEI +  G + T  D    S+L+ M  V+ E LRLY     + R+A  +I +  +
Sbjct: 351 QEKLREEIFKECGKEKTP-DSETFSKLKLMNMVIMESLRLYGPVSALAREASVNIKLGDL 410

Query: 304 TIPNGTNMWIDVVGMHHDQALWGDQVNEFHPERFQNDAIAGGCSHKMGYLPFGFGGRMCV 363
            IP GT + I ++ MH D+ LWG   ++F+P RF N  ++   +H    L F  G R C+
Sbjct: 411 EIPKGTTVVIPLLKMHSDKTLWGSDADKFNPMRFAN-GVSRAANHPNALLAFSVGPRACI 470

Query: 364 GRHLTFMEYKIVLTLILSRFSF-SLSPDYCHSPSIMLSLRPAHGLPLIFQLLED 412
           G++   +E K VLT+IL RF F SL  +Y H+P   ++++P +GLP++ Q LED
Sbjct: 471 GQNFVMIEAKTVLTMILQRFRFISLCDEYKHTPVDNVTIQPQYGLPVMLQPLED 519

[Close]

BLAST of Lag0030666 vs. ExPASy Swiss-Prot
Match: B9G934 (Cytochrome P450 714C3 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP714C3 PE=3 SV=2)

HSP 1 Score: 251.9 bits (642), Expect = 1.2e-65
Identity = 135/406 (33.25%), Postives = 221/406 (54.43%), Query Frame = 0


Query: 3   HPEFVKILSTEVKAKFWGKPSVFKRDRKSMFGNGLVMAEGDEWVRQRHVITPAFNPSNLK 62
           HP+ VK +     ++  GKP+  K+ RK++FG GL    GDEW  QR +I P F    +K
Sbjct: 108 HPDMVKDIGRWTPSEL-GKPNYLKKSRKALFGGGLFTENGDEWAYQRKIIAPEFFMDKIK 167

Query: 63  AMSSLMVESTTHMLERWANLV--LSGHPQIEVEKEITSTAGEIIAKTSFGIDHKSGRQVF 122
            M  L+ ++T  +LE W +++    G  +I V+  + + + ++IA+  FG     G ++F
Sbjct: 168 GMIQLIEDATVPVLEAWEDMIDDEGGCREIVVDDYLRNLSADVIARACFGSSFTKGEEIF 227

Query: 123 QKLRDLQMTLFKTNRLVGV-PFAGVLNAGKAREARRLGEEIDGLFRAVITARRENQAVPQ 182
            KLR LQ  + + +  VG+      L    ++E + L E++  L   ++   +E      
Sbjct: 228 CKLRQLQKAIARQDSFVGLSALWKYLPTKSSQEIQMLDEQVRLL---ILDVAKEQHHYQD 287

Query: 183 QHDLLSLLLKESGEEGKLGRRLSTTELIDECKTFFFGGHETTALALTWTLLLLGIHTEWQ 242
            H+ L   + +  ++G+     +   ++  CKT +FGGHE+TA+   W L+LL  H EWQ
Sbjct: 288 SHNSLVNAIIDGAQDGRSAAE-AEDFIVGNCKTIYFGGHESTAVTAIWCLMLLATHPEWQ 347

Query: 243 TQLREEIKQVLGDKHTDFDFTKLSQLRKMGWVMSEVLRLYPSAPNVQRQARRDITINGVT 302
            + R E  +V   + T  D   L +L+ +  V+ E LRLYP A  + R+A  D+ +  + 
Sbjct: 348 ERARAEAMEVCRGRST-LDVDALRRLKIVTMVIQETLRLYPPASVMMREALTDVKLGSIE 407

Query: 303 IPNGTNMWIDVVGMHHDQALWGDQVNEFHPERFQNDAIAGGCSHKMGYLPFGFGGRMCVG 362
           +P GT + +  + +H D+  WG   +EF P+RF N  +A  C     Y+PFG G R C+G
Sbjct: 408 VPRGTIVQVPRLMLHLDKEAWGADADEFRPDRFAN-GVAAACRAAHMYVPFGHGPRTCIG 467

Query: 363 RHLTFMEYKIVLTLILSRFSFSLSPDYCHSPSIMLSLRPAHGLPLI 406
           ++L   E K+VL  +L++F+FS SP Y HSP+  L++ P  GLPL+
Sbjct: 468 QNLAMAELKVVLARLLTKFAFSPSPRYRHSPAFRLTIEPGFGLPLM 506

[Close]

BLAST of Lag0030666 vs. ExPASy TrEMBL
Match: A0A6J1H0W5 (cytokinin hydroxylase-like OS=Cucurbita moschata OX=3662 GN=LOC111459387 PE=3 SV=1)

HSP 1 Score: 753.8 bits (1945), Expect = 3.7e-214
Identity = 369/413 (89.35%), Postives = 392/413 (94.92%), Query Frame = 0


Query: 1   MVHPEFVKILSTEVKAKFWGKPSVFKRDRKSMFGNGLVMAEGDEWVRQRHVITPAFNPSN 60
           +  PEFVK+LS EV+AK+WGKPSVFKRDRKSMFGNGLVMAEGDEWVRQRHVITPAFNPSN
Sbjct: 104 VAEPEFVKMLSREVQAKYWGKPSVFKRDRKSMFGNGLVMAEGDEWVRQRHVITPAFNPSN 163

Query: 61  LKAMSSLMVESTTHMLERWANLVLSGHPQIEVEKEITSTAGEIIAKTSFGIDHKSGRQVF 120
           LKAM+SLMVESTT ML+RWA+LVLSGHPQIEVE EITSTAGEIIAKTSFGIDH SGRQVF
Sbjct: 164 LKAMASLMVESTTQMLDRWAHLVLSGHPQIEVENEITSTAGEIIAKTSFGIDHISGRQVF 223

Query: 121 QKLRDLQMTLFKTNRLVGVPFAGVLNAGKAREARRLGEEIDGLFRAVITARRENQA---V 180
            KLR+LQMTLFKTNRLVGVPFAG+LNAGKAREA+RLGEEID LFR VITARRENQA   V
Sbjct: 224 HKLRNLQMTLFKTNRLVGVPFAGILNAGKAREAKRLGEEIDELFREVITARRENQAAAEV 283

Query: 181 PQQHDLLSLLLKESGEEGKLGRRLSTTELIDECKTFFFGGHETTALALTWTLLLLGIHTE 240
            QQ+DLLSLLLKESG EGKLGR LSTTELIDECKTFFFGGHETTALALTWTLLLLGIHTE
Sbjct: 284 VQQNDLLSLLLKESGGEGKLGRWLSTTELIDECKTFFFGGHETTALALTWTLLLLGIHTE 343

Query: 241 WQTQLREEIKQVLGDKHTDFDFTKLSQLRKMGWVMSEVLRLYPSAPNVQRQARRDITING 300
           WQTQLR+EIK+VLGDK +DFDFTKLSQL+KMGWVMSEVLRLYPSAPNVQRQARRDIT+NG
Sbjct: 344 WQTQLRDEIKEVLGDKESDFDFTKLSQLKKMGWVMSEVLRLYPSAPNVQRQARRDITVNG 403

Query: 301 VTIPNGTNMWIDVVGMHHDQALWGDQVNEFHPERFQNDAIAGGCSHKMGYLPFGFGGRMC 360
           +TIPNGTNMWIDVV MHHD+ALWG+QVNEF PERF++D +AGGCSHKMGYLPFGFGGRMC
Sbjct: 404 LTIPNGTNMWIDVVAMHHDEALWGEQVNEFRPERFEHDGVAGGCSHKMGYLPFGFGGRMC 463

Query: 361 VGRHLTFMEYKIVLTLILSRFSFSLSPDYCHSPSIMLSLRPAHGLPLIFQLLE 411
           VGRHLTFMEYKIVLTLIL RFSF+LSPDYCHSP IMLSLRPAHGLPLIFQLLE
Sbjct: 464 VGRHLTFMEYKIVLTLILCRFSFTLSPDYCHSPCIMLSLRPAHGLPLIFQLLE 516

[Close]

BLAST of Lag0030666 vs. ExPASy TrEMBL
Match: A0A6J1K6M8 (cytokinin hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LOC111491149 PE=3 SV=1)

HSP 1 Score: 740.3 bits (1910), Expect = 4.2e-210
Identity = 363/417 (87.05%), Postives = 389/417 (93.29%), Query Frame = 0


Query: 1   MVHPEFVKILSTEVKAKFWGKPSVFKRDRKSMFGNGLVMAEGDEWVRQRHVITPAFNPSN 60
           +  PEFVK+LS EV+AK+WGKPSVFKRDRKSMFGNGLVMAEGDEWVRQRHVITPAFNPSN
Sbjct: 104 LADPEFVKMLSREVQAKYWGKPSVFKRDRKSMFGNGLVMAEGDEWVRQRHVITPAFNPSN 163

Query: 61  LKAMSSLMVESTTHMLERWANLVLSGHPQIEVEKEITSTAGEIIAKTSFGIDHKSGRQVF 120
           LKAM+SLMVESTT ML+RWA+LVLSGHPQIEVE EITSTAGEIIAKTSFGIDH SGRQVF
Sbjct: 164 LKAMASLMVESTTQMLDRWAHLVLSGHPQIEVENEITSTAGEIIAKTSFGIDHISGRQVF 223

Query: 121 QKLRDLQMTLFKTNRLVGVPFAGVLNAGKAREARRLGEEIDGLFRAVITARRENQA---- 180
            KLR+LQMTLFKTNRLVGVPFAG+LNAGKAREA+RLGEEID LFR VITARRENQ+    
Sbjct: 224 HKLRNLQMTLFKTNRLVGVPFAGILNAGKAREAKRLGEEIDELFREVITARRENQSATAA 283

Query: 181 --VPQQHDLLSLLLKESGEEGKLGRRLSTTELIDECKTFFFGGHETTALALTWTLLLLGI 240
             V  Q+DLLSLLLKESG EGKL R LST ELIDECKTFFFGGHETTALALTWTLLLLGI
Sbjct: 284 AEVVLQNDLLSLLLKESGGEGKLRRWLSTAELIDECKTFFFGGHETTALALTWTLLLLGI 343

Query: 241 HTEWQTQLREEIKQVLGDKHTDFDFTKLSQLRKMGWVMSEVLRLYPSAPNVQRQARRDIT 300
           HTEWQ QLR+EIK+VLGDK +DFDFTKLSQL+KMGWVMSEVLRLYPSAPNVQRQARRDIT
Sbjct: 344 HTEWQIQLRDEIKEVLGDKESDFDFTKLSQLKKMGWVMSEVLRLYPSAPNVQRQARRDIT 403

Query: 301 INGVTIPNGTNMWIDVVGMHHDQALWGDQVNEFHPERFQNDAIAGGCSHKMGYLPFGFGG 360
           +NG+TIPNGTNMWIDVV MHHD+ALWG+QVNEF PERF++D +AGGCSHKMGYLPFGFGG
Sbjct: 404 VNGLTIPNGTNMWIDVVAMHHDEALWGEQVNEFRPERFEHDEVAGGCSHKMGYLPFGFGG 463

Query: 361 RMCVGRHLTFMEYKIVLTLILSRFSFSLSPDYCHSPSIMLSLRPAHGLPLIFQLLED 412
           RMCVGRHLTFMEYKIVLTLIL RFSF+LSPDYCHSP IMLSLRPAHGLPLIF+LLE+
Sbjct: 464 RMCVGRHLTFMEYKIVLTLILCRFSFTLSPDYCHSPCIMLSLRPAHGLPLIFRLLEN 520

[Close]

BLAST of Lag0030666 vs. ExPASy TrEMBL
Match: A0A6J1E0B2 (cytokinin hydroxylase-like OS=Momordica charantia OX=3673 GN=LOC111025977 PE=3 SV=1)

HSP 1 Score: 698.4 bits (1801), Expect = 1.8e-197
Identity = 339/410 (82.68%), Postives = 373/410 (90.98%), Query Frame = 0


Query: 1   MVHPEFVKILSTEVKAKFWGKPSVFKRDRKSMFGNGLVMAEGDEWVRQRHVITPAFNPSN 60
           +  PEF+K LSTEVKAKFWGKPSVFK DRKSMFGNGLVMAEGD+WVRQRHVITPAFNPSN
Sbjct: 106 IAEPEFLKTLSTEVKAKFWGKPSVFKDDRKSMFGNGLVMAEGDQWVRQRHVITPAFNPSN 165

Query: 61  LKAMSSLMVESTTHMLERWANLVLSGHPQIEVEKEITSTAGEIIAKTSFGIDHKSGRQVF 120
           LKAM SLMVESTT MLERWA ++ SG  +IEVE+EIT+TAGEIIAKTSFGIDH+SGRQVF
Sbjct: 166 LKAMGSLMVESTTEMLERWARMINSGDREIEVEREITATAGEIIAKTSFGIDHRSGRQVF 225

Query: 121 QKLRDLQMTLFKTNRLVGVPFAGVLNAGKAREARRLGEEIDGLFRAVITARRENQAVPQQ 180
           +KLR LQMTLFKTNRLVGVPF  +LNAGKAREA RLGEEIDGLF +VITARRE      Q
Sbjct: 226 EKLRALQMTLFKTNRLVGVPFGRLLNAGKAREAERLGEEIDGLFESVITARREGNNNQVQ 285

Query: 181 HDLLSLLLKESGEEGKLGRRLSTTELIDECKTFFFGGHETTALALTWTLLLLGIHTEWQT 240
            DLLSLLL++ G     GRRLSTTEL+DECKTFFFGGHETTALA+TWTLLLLGIHTEWQT
Sbjct: 286 QDLLSLLLRDDG-----GRRLSTTELVDECKTFFFGGHETTALAVTWTLLLLGIHTEWQT 345

Query: 241 QLREEIKQVLGDKHTDFDFTKLSQLRKMGWVMSEVLRLYPSAPNVQRQARRDITINGVTI 300
           QLREE+K+VLG+K ++FDF+ L+ L+KMGWVM EVLRLYPSAPNVQRQAR+DITING+TI
Sbjct: 346 QLREEVKEVLGNKESEFDFSNLAGLKKMGWVMREVLRLYPSAPNVQRQARKDITINGLTI 405

Query: 301 PNGTNMWIDVVGMHHDQALWGDQVNEFHPERFQNDAIAGGCSHKMGYLPFGFGGRMCVGR 360
           P+GTNMWIDVV MHHD+ALWGD+VNEFHPERF+NDA+AGGC+HKMGYLPFGFGGRMCVGR
Sbjct: 406 PDGTNMWIDVVAMHHDEALWGDKVNEFHPERFENDAVAGGCTHKMGYLPFGFGGRMCVGR 465

Query: 361 HLTFMEYKIVLTLILSRFSFSLSPDYCHSPSIMLSLRPAHGLPLIFQLLE 411
           HLTFMEYKI++ LIL RFSFSLSP Y HSPSIMLSLRPAHGLPLIFQLLE
Sbjct: 466 HLTFMEYKIMVALILCRFSFSLSPAYRHSPSIMLSLRPAHGLPLIFQLLE 510

[Close]

BLAST of Lag0030666 vs. ExPASy TrEMBL
Match: E5GCU7 (Cytochrome p450 OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1)

HSP 1 Score: 695.3 bits (1793), Expect = 1.6e-196
Identity = 346/416 (83.17%), Postives = 374/416 (89.90%), Query Frame = 0


Query: 4   PEFVKILSTEVKAKFWGKPSVFKRDRKSMFGNGLVMAEGDEWVRQRHVITPAFNPSNLKA 63
           PEFVKILS EVKAK WGKPSVFK DRKSMFGNGLVMAEGDEWVRQRH+ITPAFNPSNLKA
Sbjct: 97  PEFVKILSKEVKAKNWGKPSVFKNDRKSMFGNGLVMAEGDEWVRQRHIITPAFNPSNLKA 156

Query: 64  MSSLMVESTTHMLERWANLVLSGHPQIEVEKEITSTAGEIIAKTSFGIDHKSGRQVFQKL 123
           M+SLMVES T M+ERWANLV+SG+PQIEVE+EITSTAGEIIAKTSFGI H+SGRQVF KL
Sbjct: 157 MASLMVESATKMVERWANLVISGNPQIEVEQEITSTAGEIIAKTSFGIHHESGRQVFNKL 216

Query: 124 RDLQMTLFKTNRLVGVPFAGVLNAGKAREARRLGEEIDGLFRAVITARREN-----QAVP 183
           R LQ+TLFKTNRLVGVPF G+LNA KAREA+ LGEEID LFR+VIT RRE+         
Sbjct: 217 RQLQITLFKTNRLVGVPFVGLLNAAKAREAKLLGEEIDELFRSVITERREDAVAERTTTE 276

Query: 184 QQHDLLSLLLKES--GEEGKLGRRLSTTELIDECKTFFFGGHETTALALTWTLLLLGIHT 243
           QQ+DLLSLLLKES  G  G+ GRRLST ELIDECKTFFFGGHETTALALTWTL+LL +H+
Sbjct: 277 QQNDLLSLLLKESSGGGGGEEGRRLSTAELIDECKTFFFGGHETTALALTWTLMLLAVHS 336

Query: 244 EWQTQLREEIKQVLG-DKHTDFDFTKLSQLRKMGWVMSEVLRLYPSAPNVQRQARRDITI 303
           EWQT LREEIK+V G +  T FDFT LS L+KMGWVMSEVLRLYPSAPNVQRQAR+DI I
Sbjct: 337 EWQTILREEIKEVFGHNLQTHFDFTNLSSLKKMGWVMSEVLRLYPSAPNVQRQARKDIII 396

Query: 304 NGVTIPNGTNMWIDVVGMHHDQALWGDQVNEFHPERFQNDAIAGGCSHKMGYLPFGFGGR 363
           NG+TIPNGTNMWIDVV MHHDQALWG QVNEFHPERF+ND ++GGC+HKMGYLPFGFGGR
Sbjct: 397 NGLTIPNGTNMWIDVVSMHHDQALWGHQVNEFHPERFRNDTVSGGCTHKMGYLPFGFGGR 456

Query: 364 MCVGRHLTFMEYKIVLTLILSRFSFSLSPDYCHSPSIMLSLRPAHGLPLIFQLLED 412
           MCVGRHLTFMEYKIVLTLILSRFSFSLSPDY HSPSIMLSLRPAHGLPL+F+LLED
Sbjct: 457 MCVGRHLTFMEYKIVLTLILSRFSFSLSPDYRHSPSIMLSLRPAHGLPLVFRLLED 512

[Close]

BLAST of Lag0030666 vs. ExPASy TrEMBL
Match: A0A1S4DS94 (cytokinin hydroxylase-like OS=Cucumis melo OX=3656 GN=LOC103482547 PE=3 SV=1)

HSP 1 Score: 695.3 bits (1793), Expect = 1.6e-196
Identity = 346/416 (83.17%), Postives = 374/416 (89.90%), Query Frame = 0


Query: 4   PEFVKILSTEVKAKFWGKPSVFKRDRKSMFGNGLVMAEGDEWVRQRHVITPAFNPSNLKA 63
           PEFVKILS EVKAK WGKPSVFK DRKSMFGNGLVMAEGDEWVRQRH+ITPAFNPSNLKA
Sbjct: 124 PEFVKILSKEVKAKNWGKPSVFKNDRKSMFGNGLVMAEGDEWVRQRHIITPAFNPSNLKA 183

Query: 64  MSSLMVESTTHMLERWANLVLSGHPQIEVEKEITSTAGEIIAKTSFGIDHKSGRQVFQKL 123
           M+SLMVES T M+ERWANLV+SG+PQIEVE+EITSTAGEIIAKTSFGI H+SGRQVF KL
Sbjct: 184 MASLMVESATKMVERWANLVISGNPQIEVEQEITSTAGEIIAKTSFGIHHESGRQVFNKL 243

Query: 124 RDLQMTLFKTNRLVGVPFAGVLNAGKAREARRLGEEIDGLFRAVITARREN-----QAVP 183
           R LQ+TLFKTNRLVGVPF G+LNA KAREA+ LGEEID LFR+VIT RRE+         
Sbjct: 244 RQLQITLFKTNRLVGVPFVGLLNAAKAREAKLLGEEIDELFRSVITERREDAVAERTTTE 303

Query: 184 QQHDLLSLLLKES--GEEGKLGRRLSTTELIDECKTFFFGGHETTALALTWTLLLLGIHT 243
           QQ+DLLSLLLKES  G  G+ GRRLST ELIDECKTFFFGGHETTALALTWTL+LL +H+
Sbjct: 304 QQNDLLSLLLKESSGGGGGEEGRRLSTAELIDECKTFFFGGHETTALALTWTLMLLAVHS 363

Query: 244 EWQTQLREEIKQVLG-DKHTDFDFTKLSQLRKMGWVMSEVLRLYPSAPNVQRQARRDITI 303
           EWQT LREEIK+V G +  T FDFT LS L+KMGWVMSEVLRLYPSAPNVQRQAR+DI I
Sbjct: 364 EWQTILREEIKEVFGHNLQTHFDFTNLSSLKKMGWVMSEVLRLYPSAPNVQRQARKDIII 423

Query: 304 NGVTIPNGTNMWIDVVGMHHDQALWGDQVNEFHPERFQNDAIAGGCSHKMGYLPFGFGGR 363
           NG+TIPNGTNMWIDVV MHHDQALWG QVNEFHPERF+ND ++GGC+HKMGYLPFGFGGR
Sbjct: 424 NGLTIPNGTNMWIDVVSMHHDQALWGHQVNEFHPERFRNDTVSGGCTHKMGYLPFGFGGR 483

Query: 364 MCVGRHLTFMEYKIVLTLILSRFSFSLSPDYCHSPSIMLSLRPAHGLPLIFQLLED 412
           MCVGRHLTFMEYKIVLTLILSRFSFSLSPDY HSPSIMLSLRPAHGLPL+F+LLED
Sbjct: 484 MCVGRHLTFMEYKIVLTLILSRFSFSLSPDYRHSPSIMLSLRPAHGLPLVFRLLED 539

[Close]

BLAST of Lag0030666 vs. TAIR 10
Match: AT5G52400.1 (cytochrome P450, family 715, subfamily A, polypeptide 1 )

HSP 1 Score: 496.5 bits (1277), Expect = 2.0e-140
Identity = 242/408 (59.31%), Postives = 307/408 (75.25%), Query Frame = 0


Query: 4   PEFVKILSTEVKAKFWGKPSVFKRDRKSMFGNGLVMAEGDEWVRQRHVITPAFNPSNLKA 63
           PEF+ ++S  V  K WGKP+VFK+DR+ MFG GLVM EGD+W R RH+ITPAF P NLK 
Sbjct: 120 PEFLSVMSKGVLGKSWGKPNVFKKDREPMFGTGLVMVEGDDWTRHRHIITPAFAPLNLKV 179

Query: 64  MSSLMVESTTHMLERWANLVLSGHPQIEVEKEITSTAGEIIAKTSFGIDHKSGRQVFQKL 123
           M+++MVES ++ML+RW   + SG+P+ ++E EI  TAGEIIAKTSFG+  ++G QV + L
Sbjct: 180 MTNMMVESVSNMLDRWGIQINSGNPEFDMESEIIGTAGEIIAKTSFGVTGENGTQVLKNL 239

Query: 124 RDLQMTLFKTNRLVGVPFAGVLNAGKAREARRLGEEIDGLFRAVITARRENQAV--PQQH 183
           R +Q  LF +NR VGVPF+ +L+  +  +A+ LG EIDGL  + I  R+ + A    Q H
Sbjct: 240 RAVQFALFNSNRYVGVPFSNILSYKQTVKAKGLGHEIDGLLLSFINKRKISLAEGDDQGH 299

Query: 184 DLLSLLLKESGEEGKLGRRLSTTELIDECKTFFFGGHETTALALTWTLLLLGIHTEWQTQ 243
           DLL +LLK + ++G      +  EL+DECKTFFF GHETTALALTWT +LL IH EWQ  
Sbjct: 300 DLLGMLLK-ADQKG----NFTAKELVDECKTFFFAGHETTALALTWTFMLLAIHPEWQDT 359

Query: 244 LREEIKQVLGDKHTDFDFTKLSQLRKMGWVMSEVLRLYPSAPNVQRQARRDITINGVTIP 303
           +REEI++V+GD  +  ++ KL+ L+KM WVM+EVLRLYP APN QRQAR DI +NG  IP
Sbjct: 360 IREEIREVIGD--SKIEYNKLAGLKKMSWVMNEVLRLYPPAPNAQRQARNDIEVNGRVIP 419

Query: 304 NGTNMWIDVVGMHHDQALWGDQVNEFHPERFQNDAIAGGCSHKMGYLPFGFGGRMCVGRH 363
           NGTN+WIDVV MHHD  LWGD VNEF PERF  + + GGC +KMGY+PFGFGGRMC+GR+
Sbjct: 420 NGTNIWIDVVAMHHDVELWGDDVNEFKPERFDGN-LHGGCKNKMGYMPFGFGGRMCIGRN 479

Query: 364 LTFMEYKIVLTLILSRFSFSLSPDYCHSPSIMLSLRPAHGLPLIFQLL 410
           LT MEYKIVL+L+LSRF  S+SP Y HSP+ MLSLRP +GLPLI + L
Sbjct: 480 LTTMEYKIVLSLVLSRFEISVSPGYRHSPTYMLSLRPGYGLPLIIRPL 519

[Close]

BLAST of Lag0030666 vs. TAIR 10
Match: AT5G38450.1 (cytochrome P450, family 735, subfamily A, polypeptide 1 )

HSP 1 Score: 265.4 bits (677), Expect = 7.7e-71
Identity = 142/395 (35.95%), Postives = 227/395 (57.47%), Query Frame = 0


Query: 20  GKPSVFKRDRKSMFGNGLVMAEGDEWVRQRHVITPAFNPSNLKAMSSLMVESTTHMLERW 79
           G+  + ++  K+  G GL+MA G +W  QRH+  PAF    LK  +  MVE T+ ++ER 
Sbjct: 127 GRSWLQQQGTKNFIGRGLLMANGQDWHHQRHLAAPAFTGERLKGYARHMVECTSKLVERL 186

Query: 80  ANLVLSGHPQIEVEKEITSTAGEIIAKTSFGIDHKSGRQVFQKLRDLQMTLFKTNRLVGV 139
              V  G  ++E+ +E+     +II++T FG   + G+++F  L  LQ    +  R +  
Sbjct: 187 RKEVGEGANEVEIGEEMHKLTADIISRTKFGSSFEKGKELFNHLTVLQRRCAQATRHLCF 246

Query: 140 PFAGVLNAGKAREARRLGEEIDGLFRAVITARRE----NQAVPQQHDLLSLLLKESGEEG 199
           P +  L +   RE + L +E++ L   +I +RR+     ++     DLL LLL E   + 
Sbjct: 247 PGSRFLPSKYNREIKSLKKEVERLLIEIIQSRRDCAEMGRSSTHGDDLLGLLLNEMDIDK 306

Query: 200 KLGRRLSTTELI-DECKTFFFGGHETTALALTWTLLLLGIHTEWQTQLREEIKQVLGDKH 259
                 +  +LI DECKTFFF GHETTAL LTWT +LL  +  WQ ++REE+++V G   
Sbjct: 307 NNNNNNNNLQLIMDECKTFFFAGHETTALLLTWTTMLLADNPTWQEKVREEVREVFGRNG 366

Query: 260 TDFDFTKLSQLRKMGWVMSEVLRLYPSAPNVQRQARRDITINGVTIPNGTNMWIDVVGMH 319
                 +LS+L  +  V++E LRLYP A  + R A  D+ +  +TIP G ++WI V+ +H
Sbjct: 367 LP-SVDQLSKLTSLSKVINESLRLYPPATLLPRMAFEDLKLGDLTIPKGLSIWIPVLAIH 426

Query: 320 HDQALWGDQVNEFHPERFQNDAIAGGCSHKMGYLPFGFGGRMCVGRHLTFMEYKIVLTLI 379
           H + LWG   N+F+PERF     A G      ++PF  G R C+G+    ME KI+L  +
Sbjct: 427 HSEELWGKDANQFNPERFGGRPFASG----RHFIPFAAGPRNCIGQQFALMEAKIILATL 486

Query: 380 LSRFSFSLSPDYCHSPSIMLSLRPAHGLPLIFQLL 410
           +S+F+F++S +Y H+P ++L+++P +G+ +I + L
Sbjct: 487 ISKFNFTISKNYRHAPIVVLTIKPKYGVQVILKPL 516

[Close]

BLAST of Lag0030666 vs. TAIR 10
Match: AT1G67110.1 (cytochrome P450, family 735, subfamily A, polypeptide 2 )

HSP 1 Score: 258.5 bits (659), Expect = 9.4e-69
Identity = 141/395 (35.70%), Postives = 221/395 (55.95%), Query Frame = 0


Query: 20  GKPSVFKRDRKSMFGNGLVMAEGDEWVRQRHVITPAFNPSNLKAMSSLMVESTTHMLERW 79
           GK  + ++  K   G GL+MA G+ W  QRH+  PAF    LK  +  MVE T  M ER 
Sbjct: 128 GKSWLQQQGTKGFIGRGLLMANGEAWHHQRHMAAPAFTRDRLKGYAKHMVECTKMMAERL 187

Query: 80  ANLVLSGHPQIEVEKEITSTAGEIIAKTSFGIDHKSGRQVFQKLRDLQMTLFKTNRLVGV 139
              V     ++E+ +E+     +II++T FG     G+++F  L  LQ    +  R +  
Sbjct: 188 RKEV---GEEVEIGEEMRRLTADIISRTEFGSSCDKGKELFSLLTVLQRLCAQATRHLCF 247

Query: 140 PFAGVLNAGKAREARRLGEEIDGLFRAVITARRENQAVPQQ----HDLLSLLLKESGEEG 199
           P +  L +   RE + L  E++ L   +I +R+++  + +      DLL LLL +     
Sbjct: 248 PGSRFLPSKYNREIKSLKTEVERLLMEIIDSRKDSVEIGRSSSYGDDLLGLLLNQMDSN- 307

Query: 200 KLGRRLSTTELIDECKTFFFGGHETTALALTWTLLLLGIHTEWQTQLREEIKQVLGDKHT 259
                L+   ++DECKTFFF GHETT+L LTWTL+LL  +  WQ  +R+E++QV G    
Sbjct: 308 --KNNLNVQMIMDECKTFFFTGHETTSLLLTWTLMLLAHNPTWQDNVRDEVRQVCGQDGV 367

Query: 260 DFDFTKLSQLRKMGWVMSEVLRLYPSAPNVQRQARRDITINGVTIPNGTNMWIDVVGMHH 319
                +LS L  +  V++E LRLYP A  + R A  DI +  + IP G ++WI V+ +HH
Sbjct: 368 P-SVEQLSSLTSLNKVINESLRLYPPATLLPRMAFEDIKLGDLIIPKGLSIWIPVLAIHH 427

Query: 320 DQALWGDQVNEFHPERFQNDAIAGGCSHKMGYLPFGFGGRMCVGRHLTFMEYKIVLTLIL 379
              LWG+  NEF+PERF   + A        ++PF  G R C+G+    ME KI+L +++
Sbjct: 428 SNELWGEDANEFNPERFTTRSFAS----SRHFMPFAAGPRNCIGQTFAMMEAKIILAMLV 487

Query: 380 SRFSFSLSPDYCHSPSIMLSLRPAHGLPLIFQLLE 411
           S+FSF++S +Y H+P ++L+++P +G+ L+ + L+
Sbjct: 488 SKFSFAISENYRHAPIVVLTIKPKYGVQLVLKPLD 511

[Close]

BLAST of Lag0030666 vs. TAIR 10
Match: AT3G14620.1 (cytochrome P450, family 72, subfamily A, polypeptide 8 )

HSP 1 Score: 253.4 bits (646), Expect = 3.0e-67
Identity = 134/384 (34.90%), Postives = 220/384 (57.29%), Query Frame = 0


Query: 32  MFGNGLVMAEGDEWVRQRHVITPAFNPSNLKAMSSLMVESTTHMLERWANLVL--SGHPQ 91
           +F  G+ + EG++W + R +I P+F+   LK M     ES + M+ +W  LV       +
Sbjct: 139 LFATGVALYEGEKWSKHRKIINPSFHLEKLKIMIPAFYESCSEMISKWEKLVTEQGSSNE 198

Query: 92  IEVEKEITSTAGEIIAKTSFGIDHKSGRQVFQKLRDLQMTLFKTNRLVGVPFAGVLNAGK 151
           I+V   +     ++I++T+FG  ++ G+++F+   +    + K   L  +P    L    
Sbjct: 199 IDVWPYLGDLTSDVISRTAFGSSYEEGKRIFELQEEQGRRVLKALELAFIPGMRFLPTKN 258

Query: 152 AREARRLGEEIDGLFRAVITARRENQAVPQ--QHDLLSLLLK-ESGEEGKLGRRLSTTEL 211
               R++ +E+    R +I  R+      +  ++DLL +LL+  SG+ G     +S  ++
Sbjct: 259 NLRMRQINKEVKSRLREIIMKRQRGMDTGEAPKNDLLGILLESNSGDHG-----MSIEDV 318

Query: 212 IDECKTFFFGGHETTALALTWTLLLLGIHTEWQTQLREEIKQVLGDKHTDFDFTKLSQLR 271
           ++EC+ F F G ETTA+ L WT+++L  H +WQ Q REEI +V+G K+   +F  LS+L+
Sbjct: 319 VEECRLFHFAGQETTAVLLVWTMIMLSHHQKWQDQAREEILKVIG-KNNKPNFDALSRLK 378

Query: 272 KMGWVMSEVLRLYPSAPNVQRQARRDITI-NGVTIPNGTNMWIDVVGMHHDQALWGDQVN 331
            M  +++EVLRLYP    + R   ++  +   +T+P G  + I V+ +H D  LWG+ V+
Sbjct: 379 TMSMILNEVLRLYPPGILLGRTVEKETKLGEDMTLPGGAQVVIPVLMVHRDPELWGEDVH 438

Query: 332 EFHPERFQNDAIAGGCSHKMGYLPFGFGGRMCVGRHLTFMEYKIVLTLILSRFSFSLSPD 391
           EF+PERF  D I+    +++ +LPFG+G R C G++   ME K+ L LIL RFSF LSP 
Sbjct: 439 EFNPERFA-DGISKATKNQVSFLPFGWGPRFCPGQNFALMEAKMALVLILQRFSFELSPS 498

Query: 392 YCHSPSIMLSLRPAHGLPLIFQLL 410
           Y H+P  +L+L P  G PLIF +L
Sbjct: 499 YTHAPHTVLTLHPQFGAPLIFHML 515

[Close]

BLAST of Lag0030666 vs. TAIR 10
Match: AT2G46960.2 (cytochrome P450, family 709, subfamily B, polypeptide 1 )

HSP 1 Score: 253.1 bits (645), Expect = 3.9e-67
Identity = 149/414 (35.99%), Postives = 231/414 (55.80%), Query Frame = 0


Query: 4   PEFVKILSTEVKAKFWGKPSVFKRDRKSMFGNGLVMAEGDEWVRQRHVITPAFNPSNLKA 63
           PE  K + +  K  F+ K        K +   GLV  EG +WVR R ++ PAF+   LK 
Sbjct: 111 PELAKTMLSN-KLGFFVKSKARPEAVKLVGSKGLVFIEGADWVRHRRILNPAFSIDRLKI 170

Query: 64  MSSLMVESTTHMLERW---ANLVLSGHPQI--EVEKEITSTAGEIIAKTSFGIDHKSGRQ 123
           M+++MV+ T  MLE W   +    + HP+I  E+ +E      +IIA ++FG  +  G +
Sbjct: 171 MTTVMVDCTLKMLEEWRKESTKEETEHPKIKKEMNEEFQRLTADIIATSAFGSSYVEGIE 230

Query: 124 VFQKLRDLQMTLFKTNRLVGVPFAGVLNAGKAREARRLGEEIDGLFRAVITARRENQAVP 183
           VF+   +L+     +   V +P    L         +L  ++D   + +I++R ++++  
Sbjct: 231 VFRSQMELKRCYTTSLNQVSIPGTQYLPTPSNIRVWKLERKMDNSIKRIISSRLQSKS-D 290

Query: 184 QQHDLLSLLLKESGEEGKLGRRLSTTELIDECKTFFFGGHETTALALTWTLLLLGIHTEW 243
              DLL +LLK    EGK  R++S  E+I EC+TFFFGGHETT+  L WT +LL +H +W
Sbjct: 291 YGDDLLGILLKAYNTEGK-ERKMSIEEIIHECRTFFFGGHETTSNLLAWTTMLLSLHQDW 350

Query: 244 QTQLREEIKQVLGDKHTDFDFTKLSQLRKMGWVMSEVLRLYPSAPNVQRQARRDITINGV 303
           Q +LREEI +  G + T  D    S+L+ M  V+ E LRLY     + R+A  +I +  +
Sbjct: 351 QEKLREEIFKECGKEKTP-DSETFSKLKLMNMVIMESLRLYGPVSALAREASVNIKLGDL 410

Query: 304 TIPNGTNMWIDVVGMHHDQALWGDQVNEFHPERFQNDAIAGGCSHKMGYLPFGFGGRMCV 363
            IP GT + I ++ MH D+ LWG   ++F+P RF N  ++   +H    L F  G R C+
Sbjct: 411 EIPKGTTVVIPLLKMHSDKTLWGSDADKFNPMRFAN-GVSRAANHPNALLAFSVGPRACI 470

Query: 364 GRHLTFMEYKIVLTLILSRFSF-SLSPDYCHSPSIMLSLRPAHGLPLIFQLLED 412
           G++   +E K VLT+IL RF F SL  +Y H+P   ++++P +GLP++ Q LED
Sbjct: 471 GQNFVMIEAKTVLTMILQRFRFISLCDEYKHTPVDNVTIQPQYGLPVMLQPLED 519

The following BLAST results are available for this feature:

BLAST of Lag0030666 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022958036.1	7.6e-214	89.35	cytokinin hydroxylase-like [Cucurbita moschata]	[more]
KAG7035636.1	7.6e-214	90.24	Cytochrome P450, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_023533013.1	5.5e-212	88.01	cytokinin hydroxylase-like [Cucurbita pepo subsp. pepo]	[more]
XP_022995689.1	8.7e-210	87.05	cytokinin hydroxylase-like [Cucurbita maxima]	[more]
KAG6605729.1	6.9e-199	84.99	Cytochrome P450 72A11, partial [Cucurbita argyrosperma subsp. sororia]	[more]

BLAST of Lag0030666 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q2QYH7	8.0e-73	36.03	Cytochrome P450 714C2 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP714C2 PE=2 ...	[more]
Q9FF18	1.1e-69	35.95	Cytokinin hydroxylase OS=Arabidopsis thaliana OX=3702 GN=CYP735A1 PE=1 SV=1	[more]
Q9ZW95	1.3e-67	35.70	Cytokinin hydroxylase OS=Arabidopsis thaliana OX=3702 GN=CYP735A2 PE=1 SV=1	[more]
Q9ASR3	5.5e-66	35.99	Cytochrome P450 709B1 OS=Arabidopsis thaliana OX=3702 GN=CYP709B1 PE=2 SV=1	[more]
B9G934	1.2e-65	33.25	Cytochrome P450 714C3 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP714C3 PE=3 ...	[more]

BLAST of Lag0030666 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1H0W5	3.7e-214	89.35	cytokinin hydroxylase-like OS=Cucurbita moschata OX=3662 GN=LOC111459387 PE=3 SV...	[more]
A0A6J1K6M8	4.2e-210	87.05	cytokinin hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LOC111491149 PE=3 SV=1	[more]
A0A6J1E0B2	1.8e-197	82.68	cytokinin hydroxylase-like OS=Momordica charantia OX=3673 GN=LOC111025977 PE=3 S...	[more]
E5GCU7	1.6e-196	83.17	Cytochrome p450 OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1	[more]
A0A1S4DS94	1.6e-196	83.17	cytokinin hydroxylase-like OS=Cucumis melo OX=3656 GN=LOC103482547 PE=3 SV=1	[more]

BLAST of Lag0030666 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G52400.1	2.0e-140	59.31	cytochrome P450, family 715, subfamily A, polypeptide 1	[more]
AT5G38450.1	7.7e-71	35.95	cytochrome P450, family 735, subfamily A, polypeptide 1	[more]
AT1G67110.1	9.4e-69	35.70	cytochrome P450, family 735, subfamily A, polypeptide 2	[more]
AT3G14620.1	3.0e-67	34.90	cytochrome P450, family 72, subfamily A, polypeptide 8	[more]
AT2G46960.2	3.9e-67	35.99	cytochrome P450, family 709, subfamily B, polypeptide 1	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001128	Cytochrome P450	PRINTS	PR00385	P450	coord: 270..281 score: 39.82 coord: 348..357 score: 57.77 coord: 216..233 score: 39.43 coord: 357..368 score: 34.73
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 4..388 e-value: 2.8E-74 score: 250.6
IPR002401	Cytochrome P450, E-class, group I	PRINTS	PR00463	EP450I	coord: 205..222 score: 27.5 coord: 357..380 score: 32.68 coord: 347..357 score: 51.95 coord: 225..251 score: 34.76
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 2..409 e-value: 3.3E-99 score: 334.6
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 6..406
None	No IPR available	PANTHER	PTHR24282:SF149	CYTOCHROME P450 FAMILY 72 PROTEIN	coord: 2..405
None	No IPR available	PANTHER	PTHR24282	CYTOCHROME P450 FAMILY MEMBER	coord: 2..405
IPR017972	Cytochrome P450, conserved site	PROSITE	PS00086	CYTOCHROME_P450	coord: 350..359

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0030666.1	Lag0030666.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen