Lag0030660 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0030660 vs. NCBI nr
Match: XP_038888501.1 (low-temperature-induced 65 kDa protein isoform X1 [Benincasa hispida])

HSP 1 Score: 484.2 bits (1245), Expect = 1.2e-132
Identity = 302/464 (65.09%), Postives = 346/464 (74.57%), Query Frame = 0

Query: 1   MDSQQINRHDH-----ALHQSVGGEED-----HEKKSVLKKVKAKAKKIKDTITKHGHG- 60
           MDS QI  H H     ALHQSV G+E+     HEKKSVLKKVKAKAKKIKDTITKHGHG 
Sbjct: 1   MDS-QIAPHHHLHHPLALHQSVEGKEEEDGQLHEKKSVLKKVKAKAKKIKDTITKHGHGH 60

Query: 61  -HDHHDYEDEEDEDEDEGVVEDPQLQEAPLYEGAAMRSAAVAGQGKHQDVGIGMSTTTHN 120
            HDHHDYEDE+DEDED+ V+EDP++Q APLYEGAAMRS AVAGQG+HQDVGIGM+T  HN
Sbjct: 61  DHDHHDYEDEDDEDEDDEVIEDPEIQGAPLYEGAAMRS-AVAGQGQHQDVGIGMTTGMHN 120

Query: 121 HNQAAPRETTTRPSAVDTAFTYVNAPTT-----KVDNSSVAPNTTMSLSPWSLEEDPHAP 180
                PRETT+RPSA DT FT V+ PTT     KV++S+VAPNTTMSLSPW LEEDPH  
Sbjct: 121 EPSRGPRETTSRPSAFDTGFTSVDNPTTNKGADKVEDSAVAPNTTMSLSPWKLEEDPHG- 180

Query: 181 KDKTHPNAPPNSQVKVSDPARRGN-----SQIFDSFAKMKVNEEHEPNRTGLSSGIKQGG 240
                P+ P NSQVKV DPA RG+     SQ+FDSFAKMKVN+E+EPNR  L+S I +GG
Sbjct: 181 -----PHTPHNSQVKVHDPANRGSEEAGKSQVFDSFAKMKVNDEYEPNRPSLASRIDRGG 240

Query: 241 E-ESSYTQKISGVGCAVSGKAVAAKDYVASKLGYGETTEDNTNTNPKSCSPLEYGKKIAL 300
           E +++Y QK+S VG AVS KAVAAKD+VASKLGY ETTE+  N    S SPLEYGKKIAL
Sbjct: 241 EDQTNYGQKVSAVGSAVSEKAVAAKDFVASKLGYVETTEETIN---NSSSPLEYGKKIAL 300

Query: 301 TVTEKLKPGEEDKALSEVISEAINRRKEEVVKVGESAFRQ-----PKGKVTESEGLTRRL 360
           TVTEKLKPGEEDKALSEVISEA++RRK E+VKVGESAF +      KG+VTESE LTRRL
Sbjct: 301 TVTEKLKPGEEDKALSEVISEALSRRKYELVKVGESAFGRHQSLLSKGEVTESEELTRRL 360

Query: 361 GRED-----------SAPATGWSVVDKVKDTVGTWLGKAGDQSSPSQQSVGTSQGVEGFM 420
           G+ED           +  ATG SVV  VKDTVG+WLGKAG+QS+PSQQS+G SQGVEGF+
Sbjct: 361 GKEDKEATEKSSVAGATAATGRSVVGMVKDTVGSWLGKAGEQSAPSQQSLGISQGVEGFV 420

BLAST of Lag0030660 vs. NCBI nr
Match: XP_008465872.2 (PREDICTED: low-temperature-induced 65 kDa protein [Cucumis melo])

HSP 1 Score: 477.2 bits (1227), Expect = 1.4e-130
Identity = 303/458 (66.16%), Postives = 335/458 (73.14%), Query Frame = 0

Query: 1   MDSQQINRHDH----ALHQSV-------GGEEDHEKKSVLKKVKAKAKKIKDTITKHGHG 60
           MDSQ    H H     LHQS         GEE HEKKSVLKKVKAKAKKIKDTITKH H 
Sbjct: 1   MDSQIPPHHHHHHPLGLHQSAEGKEELEEGEEHHEKKSVLKKVKAKAKKIKDTITKHAHA 60

Query: 61  HDHHDYEDEEDEDEDEGVVEDPQLQEAPLYEGAAMRSAAVAGQGKHQDVGIGMSTTTHNH 120
           HDHHD EDEED++EDE VVEDP++Q APLYEGAAMRS A AG+G+HQDVGIG+ TTT  H
Sbjct: 61  HDHHDEEDEEDDEEDE-VVEDPEIQGAPLYEGAAMRS-ATAGKGQHQDVGIGIGTTTMMH 120

Query: 121 NQAAP---RETTTRPSAVDTAFTYVNAPTT--KVDNSSVAPNTTMSLSPWSLEEDPHAPK 180
           N A P   RE  +RPSAVDT FT V  PT   KVD+S+VAPNTTMSLSPW LEEDPH PK
Sbjct: 121 NYAPPPARREIRSRPSAVDTGFTSVENPTANHKVDDSNVAPNTTMSLSPWKLEEDPHGPK 180

Query: 181 DKTHPNAPPNSQVKVSDPARRGN------SQIFDSFAKMKVNEEHEPNRTGLSSGIKQ-- 240
           D   P AP  S+VKV DP+ RG+      SQ+FDSFAKMKV+++ +PNRTG   G+ Q  
Sbjct: 181 D---PRAPHISEVKVHDPSNRGSEEAAGKSQVFDSFAKMKVDDK-KPNRTGSPHGLDQEH 240

Query: 241 GGEES-SYTQKISGVGCAVSGKAVAAKDYVASKLGYGETTEDNTNTNPKSCSPLEYGKKI 300
           GGE+  +Y QKIS VG AVSGKAVAAKD+VASKLGYGET E  T     S SPLEYGKKI
Sbjct: 241 GGEDHINYAQKISAVGSAVSGKAVAAKDFVASKLGYGETKEGTTADKSSSSSPLEYGKKI 300

Query: 301 ALTVTEKLKPGEEDKALSEVISEAINRRKEEVVKVGESAFRQP--KGKVTESEGLTRRLG 360
           ALTVTEKLKPGEED+ALSEVISEA+NRRK+EVVKVGESAF +P  KG VTESE LTRRLG
Sbjct: 301 ALTVTEKLKPGEEDRALSEVISEALNRRKDEVVKVGESAFGRPPSKGVVTESEELTRRLG 360

Query: 361 RED-----------SAPATGWSVVDKVKDTVGTWLGKAGDQSSPSQQSVGTSQGVEGFMD 420
            ED           +A ATG SVV  VKDTVG+WLGKAG+QS+PSQQS+GTSQGVEGF+D
Sbjct: 361 EEDKEATEKSSVASAAAATGRSVVGMVKDTVGSWLGKAGEQSAPSQQSLGTSQGVEGFVD 420

BLAST of Lag0030660 vs. NCBI nr
Match: XP_038888502.1 (low-temperature-induced 65 kDa protein isoform X2 [Benincasa hispida])

HSP 1 Score: 470.3 bits (1209), Expect = 1.7e-128
Identity = 298/464 (64.22%), Postives = 342/464 (73.71%), Query Frame = 0

Query: 1   MDSQQINRHDH-----ALHQSVGGEED-----HEKKSVLKKVKAKAKKIKDTITKHGHG- 60
           MDS QI  H H     ALHQSV G+E+     HEKKSVLKKVKAKAKKIKDTITKHGHG 
Sbjct: 1   MDS-QIAPHHHLHHPLALHQSVEGKEEEDGQLHEKKSVLKKVKAKAKKIKDTITKHGHGH 60

Query: 61  -HDHHDYEDEEDEDEDEGVVEDPQLQEAPLYEGAAMRSAAVAGQGKHQDVGIGMSTTTHN 120
            HDHHDYEDE+DEDED+ V+EDP++Q AP    AAMRS AVAGQG+HQDVGIGM+T  HN
Sbjct: 61  DHDHHDYEDEDDEDEDDEVIEDPEIQGAPC---AAMRS-AVAGQGQHQDVGIGMTTGMHN 120

Query: 121 HNQAAPRETTTRPSAVDTAFTYVNAPTT-----KVDNSSVAPNTTMSLSPWSLEEDPHAP 180
                PRETT+RPSA DT FT V+ PTT     KV++S+VAPNTTMSLSPW LEEDPH  
Sbjct: 121 EPSRGPRETTSRPSAFDTGFTSVDNPTTNKGADKVEDSAVAPNTTMSLSPWKLEEDPHG- 180

Query: 181 KDKTHPNAPPNSQVKVSDPARRGN-----SQIFDSFAKMKVNEEHEPNRTGLSSGIKQGG 240
                P+ P NSQVKV DPA RG+     SQ+FDSFAKMKVN+E+EPNR  L+S I +GG
Sbjct: 181 -----PHTPHNSQVKVHDPANRGSEEAGKSQVFDSFAKMKVNDEYEPNRPSLASRIDRGG 240

Query: 241 E-ESSYTQKISGVGCAVSGKAVAAKDYVASKLGYGETTEDNTNTNPKSCSPLEYGKKIAL 300
           E +++Y QK+S VG AVS KAVAAKD+VASKLGY ETTE+  N    S SPLEYGKKIAL
Sbjct: 241 EDQTNYGQKVSAVGSAVSEKAVAAKDFVASKLGYVETTEETIN---NSSSPLEYGKKIAL 300

Query: 301 TVTEKLKPGEEDKALSEVISEAINRRKEEVVKVGESAFRQ-----PKGKVTESEGLTRRL 360
           TVTEKLKPGEEDKALSEVISEA++RRK E+VKVGESAF +      KG+VTESE LTRRL
Sbjct: 301 TVTEKLKPGEEDKALSEVISEALSRRKYELVKVGESAFGRHQSLLSKGEVTESEELTRRL 360

Query: 361 GRED-----------SAPATGWSVVDKVKDTVGTWLGKAGDQSSPSQQSVGTSQGVEGFM 420
           G+ED           +  ATG SVV  VKDTVG+WLGKAG+QS+PSQQS+G SQGVEGF+
Sbjct: 361 GKEDKEATEKSSVAGATAATGRSVVGMVKDTVGSWLGKAGEQSAPSQQSLGISQGVEGFV 420

BLAST of Lag0030660 vs. NCBI nr
Match: XP_011652891.1 (low-temperature-induced 65 kDa protein isoform X2 [Cucumis sativus])

HSP 1 Score: 469.9 bits (1208), Expect = 2.2e-128
Identity = 298/461 (64.64%), Postives = 338/461 (73.32%), Query Frame = 0

Query: 1   MDSQQINRHDH------ALHQS---------VGGEEDHEKKSVLKKVKAKAKKIKDTITK 60
           MDSQ    H H       LHQS          G +E HEKKSVLKKVKAKAKKIKDTITK
Sbjct: 1   MDSQIPPHHHHHHHHPLGLHQSHVEGKEELQEGDQEHHEKKSVLKKVKAKAKKIKDTITK 60

Query: 61  HGHGHDHHDYEDEEDEDEDEGVVEDPQLQEAPLYEGAAMRSAAVAGQGKHQDVGIGMSTT 120
           HGHGHDHHD ED  D++EDE VVEDP++Q APLYEGAAMRS AVAG+G+HQDVGIG  TT
Sbjct: 61  HGHGHDHHDEEDVSDDEEDE-VVEDPEIQGAPLYEGAAMRS-AVAGKGQHQDVGIGTGTT 120

Query: 121 THNHNQAAP--RETTTRPSAVDTAFTYVNAPTT--KVDNSSVAPNTTMSLSPWSLEEDPH 180
              HN+  P  RE T+RPSAVDT FT ++  T   KVD+S+VAPNTTMSLSPW LE+DPH
Sbjct: 121 RMMHNEPPPARREITSRPSAVDTGFTSIHNTTANYKVDDSNVAPNTTMSLSPWKLEDDPH 180

Query: 181 APKDKTHPNAPPNSQVKVSDPARRGN------SQIFDSFAKMKVNEEHEPNRTGLSSGI- 240
           APKD   P+AP  S+VKV DP+ RG+      SQ+FDSFA+MKV+++ EPNRTG   G+ 
Sbjct: 181 APKD---PHAPHISEVKVHDPSNRGSEEAAGRSQVFDSFARMKVDDK-EPNRTGSPRGLV 240

Query: 241 -KQGGE-ESSYTQKISGVGCAVSGKAVAAKDYVASKLGYGETTEDNTNTNPKSCSPLEYG 300
            +QGGE +++Y QKIS VG AVSGKAVAAKD+VASKLGYGETTE+ T     S SPLEYG
Sbjct: 241 QEQGGEDQTNYAQKISAVGSAVSGKAVAAKDFVASKLGYGETTEETTTNKSSSSSPLEYG 300

Query: 301 KKIALTVTEKLKPGEEDKALSEVISEAINRRKEEVVKVGESAFRQP--KGKVTESEGLTR 360
           KKIALTVTEKLKPGEED+ALSEVISEA  RRK+EVVKVGESAF +P  KG+VTESE LTR
Sbjct: 301 KKIALTVTEKLKPGEEDRALSEVISEAWTRRKDEVVKVGESAFGRPPSKGEVTESEELTR 360

Query: 361 RLGRED-----------SAPATGWSVVDKVKDTVGTWLGKAGDQSSPSQQSVGTSQGVEG 420
           RLG+ED           +A ATG SVV  VKDTVG+WLG AG+QS PSQQS+GTSQGVEG
Sbjct: 361 RLGKEDKEATEKSSVARAAAATGRSVVGMVKDTVGSWLGNAGEQSVPSQQSLGTSQGVEG 420

BLAST of Lag0030660 vs. NCBI nr
Match: XP_031739118.1 (low-temperature-induced 65 kDa protein isoform X1 [Cucumis sativus])

HSP 1 Score: 468.8 bits (1205), Expect = 5.0e-128
Identity = 297/464 (64.01%), Postives = 339/464 (73.06%), Query Frame = 0

Query: 1   MDSQQINRHDH------ALHQSV------------GGEEDHEKKSVLKKVKAKAKKIKDT 60
           MDSQ    H H       LHQ++            G +E HEKKSVLKKVKAKAKKIKDT
Sbjct: 1   MDSQIPPHHHHHHHHPLGLHQTLVGHVEGKEELQEGDQEHHEKKSVLKKVKAKAKKIKDT 60

Query: 61  ITKHGHGHDHHDYEDEEDEDEDEGVVEDPQLQEAPLYEGAAMRSAAVAGQGKHQDVGIGM 120
           ITKHGHGHDHHD ED  D++EDE VVEDP++Q APLYEGAAMRS AVAG+G+HQDVGIG 
Sbjct: 61  ITKHGHGHDHHDEEDVSDDEEDE-VVEDPEIQGAPLYEGAAMRS-AVAGKGQHQDVGIGT 120

Query: 121 STTTHNHNQAAP--RETTTRPSAVDTAFTYVNAPTT--KVDNSSVAPNTTMSLSPWSLEE 180
            TT   HN+  P  RE T+RPSAVDT FT ++  T   KVD+S+VAPNTTMSLSPW LE+
Sbjct: 121 GTTRMMHNEPPPARREITSRPSAVDTGFTSIHNTTANYKVDDSNVAPNTTMSLSPWKLED 180

Query: 181 DPHAPKDKTHPNAPPNSQVKVSDPARRGN------SQIFDSFAKMKVNEEHEPNRTGLSS 240
           DPHAPKD   P+AP  S+VKV DP+ RG+      SQ+FDSFA+MKV+++ EPNRTG   
Sbjct: 181 DPHAPKD---PHAPHISEVKVHDPSNRGSEEAAGRSQVFDSFARMKVDDK-EPNRTGSPR 240

Query: 241 GI--KQGGE-ESSYTQKISGVGCAVSGKAVAAKDYVASKLGYGETTEDNTNTNPKSCSPL 300
           G+  +QGGE +++Y QKIS VG AVSGKAVAAKD+VASKLGYGETTE+ T     S SPL
Sbjct: 241 GLVQEQGGEDQTNYAQKISAVGSAVSGKAVAAKDFVASKLGYGETTEETTTNKSSSSSPL 300

Query: 301 EYGKKIALTVTEKLKPGEEDKALSEVISEAINRRKEEVVKVGESAFRQP--KGKVTESEG 360
           EYGKKIALTVTEKLKPGEED+ALSEVISEA  RRK+EVVKVGESAF +P  KG+VTESE 
Sbjct: 301 EYGKKIALTVTEKLKPGEEDRALSEVISEAWTRRKDEVVKVGESAFGRPPSKGEVTESEE 360

Query: 361 LTRRLGRED-----------SAPATGWSVVDKVKDTVGTWLGKAGDQSSPSQQSVGTSQG 420
           LTRRLG+ED           +A ATG SVV  VKDTVG+WLG AG+QS PSQQS+GTSQG
Sbjct: 361 LTRRLGKEDKEATEKSSVARAAAATGRSVVGMVKDTVGSWLGNAGEQSVPSQQSLGTSQG 420

BLAST of Lag0030660 vs. ExPASy Swiss-Prot
Match: Q04980 (Low-temperature-induced 65 kDa protein OS=Arabidopsis thaliana OX=3702 GN=LTI65 PE=2 SV=2)

HSP 1 Score: 85.5 bits (210), Expect = 1.6e-15
Identity = 156/587 (26.58%), Postives = 221/587 (37.65%), Query Frame = 0

Query: 20  EEDHEK--KSVLKKVKAKAKKIKDTITKHGHGHDHHDYEDEEDEDEDEGVVEDPQLQEAP 79
           EE HEK    VLKKVK KAKKIK+++TKHG+GHD HD ED++DE ++    +DP++  AP
Sbjct: 26  EEHHEKGASKVLKKVKEKAKKIKNSLTKHGNGHD-HDVEDDDDEYDE----QDPEVHGAP 85

Query: 80  LYEGAAMRSAAVAGQGKH---------------------------------------QDV 139
           +YE +A+R   V G+ K                                        QD 
Sbjct: 86  VYESSAVR-GGVTGKPKSLSHAGETNVPASEEIVPPGTKVFPVVSSDHTKPIEPVSLQDT 145

Query: 140 GIGMST------TTHNHNQAAPRETTTR-PSAV-------DTAFTYVNAPTTKVDNSSVA 199
             G         TT   +  A RE  T  P  V       ++   +     T V   S  
Sbjct: 146 SYGHEALADPVRTTETSDWEAKREAPTHYPLGVSEFSDRGESREAHQEPLNTPVSLLSAT 205

Query: 200 PNTTMSLSPWS------------------LEEDPHAP-KDKTHPNAPPNSQVKVSDPARR 259
            + T + +P                    LEEDP AP     + +   N Q KV+DP  +
Sbjct: 206 EDVTRTFAPGGEDDYLGGQRKVNVETPKRLEEDPAAPGGGSDYLSGVSNYQSKVTDPTHK 265

Query: 260 GNS----QIFDSFAKMKVNEE------------------HE-------------PNRTG- 319
           G      +I +S  +MKV +E                  HE             P R G 
Sbjct: 266 GGEAGVPEIAESLGRMKVTDESPDQKSRQGREEDFPTRSHEFDLKKESDINKNSPARFGG 325

Query: 320 -----------------LSSGI---------------------KQGGEE----------- 379
                            + SG+                     +   EE           
Sbjct: 326 ESKAGMEEDFPTRGDVKVESGLGRDLPTGTHDQFSPELSRPKERDDSEETKDESTHETKP 385

Query: 380 SSYTQKISGVGCAVSGKAVAAKDYVASKLGY-GETTEDNTNTNPKSCSP---LEYGKKIA 385
           S+YT++++    A++ KA+AAK+ VASKLGY GE     + +  K  +P     YG+K+A
Sbjct: 386 STYTEQLASATSAITNKAIAAKNVVASKLGYTGENGGGQSESPVKDETPRSVTAYGQKVA 445

BLAST of Lag0030660 vs. ExPASy TrEMBL
Match: A0A1S3CPX7 (low-temperature-induced 65 kDa protein OS=Cucumis melo OX=3656 GN=LOC103503460 PE=4 SV=1)

HSP 1 Score: 477.2 bits (1227), Expect = 6.8e-131
Identity = 303/458 (66.16%), Postives = 335/458 (73.14%), Query Frame = 0

Query: 1   MDSQQINRHDH----ALHQSV-------GGEEDHEKKSVLKKVKAKAKKIKDTITKHGHG 60
           MDSQ    H H     LHQS         GEE HEKKSVLKKVKAKAKKIKDTITKH H 
Sbjct: 1   MDSQIPPHHHHHHPLGLHQSAEGKEELEEGEEHHEKKSVLKKVKAKAKKIKDTITKHAHA 60

Query: 61  HDHHDYEDEEDEDEDEGVVEDPQLQEAPLYEGAAMRSAAVAGQGKHQDVGIGMSTTTHNH 120
           HDHHD EDEED++EDE VVEDP++Q APLYEGAAMRS A AG+G+HQDVGIG+ TTT  H
Sbjct: 61  HDHHDEEDEEDDEEDE-VVEDPEIQGAPLYEGAAMRS-ATAGKGQHQDVGIGIGTTTMMH 120

Query: 121 NQAAP---RETTTRPSAVDTAFTYVNAPTT--KVDNSSVAPNTTMSLSPWSLEEDPHAPK 180
           N A P   RE  +RPSAVDT FT V  PT   KVD+S+VAPNTTMSLSPW LEEDPH PK
Sbjct: 121 NYAPPPARREIRSRPSAVDTGFTSVENPTANHKVDDSNVAPNTTMSLSPWKLEEDPHGPK 180

Query: 181 DKTHPNAPPNSQVKVSDPARRGN------SQIFDSFAKMKVNEEHEPNRTGLSSGIKQ-- 240
           D   P AP  S+VKV DP+ RG+      SQ+FDSFAKMKV+++ +PNRTG   G+ Q  
Sbjct: 181 D---PRAPHISEVKVHDPSNRGSEEAAGKSQVFDSFAKMKVDDK-KPNRTGSPHGLDQEH 240

Query: 241 GGEES-SYTQKISGVGCAVSGKAVAAKDYVASKLGYGETTEDNTNTNPKSCSPLEYGKKI 300
           GGE+  +Y QKIS VG AVSGKAVAAKD+VASKLGYGET E  T     S SPLEYGKKI
Sbjct: 241 GGEDHINYAQKISAVGSAVSGKAVAAKDFVASKLGYGETKEGTTADKSSSSSPLEYGKKI 300

Query: 301 ALTVTEKLKPGEEDKALSEVISEAINRRKEEVVKVGESAFRQP--KGKVTESEGLTRRLG 360
           ALTVTEKLKPGEED+ALSEVISEA+NRRK+EVVKVGESAF +P  KG VTESE LTRRLG
Sbjct: 301 ALTVTEKLKPGEEDRALSEVISEALNRRKDEVVKVGESAFGRPPSKGVVTESEELTRRLG 360

Query: 361 RED-----------SAPATGWSVVDKVKDTVGTWLGKAGDQSSPSQQSVGTSQGVEGFMD 420
            ED           +A ATG SVV  VKDTVG+WLGKAG+QS+PSQQS+GTSQGVEGF+D
Sbjct: 361 EEDKEATEKSSVASAAAATGRSVVGMVKDTVGSWLGKAGEQSAPSQQSLGTSQGVEGFVD 420

BLAST of Lag0030660 vs. ExPASy TrEMBL
Match: A0A5D3E5L4 (Low-temperature-induced 65 kDa protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold455G003650 PE=4 SV=1)

HSP 1 Score: 387.9 bits (995), Expect = 5.4e-104
Identity = 243/370 (65.68%), Postives = 272/370 (73.51%), Query Frame = 0

Query: 78  LYEGAAMRSAAVAGQGKHQDVGIGMSTTTHNHNQAAP---RETTTRPSAVDTAFTYVNAP 137
           +YEGAAMRS A AG+G+HQDVGIG+ TTT  HN A P   RE  +RPSAVDT FT V  P
Sbjct: 8   VYEGAAMRS-ATAGKGQHQDVGIGIGTTTMMHNYAPPPARREIRSRPSAVDTGFTSVENP 67

Query: 138 TT--KVDNSSVAPNTTMSLSPWSLEEDPHAPKDKTHPNAPPNSQVKVSDPARRGN----- 197
           T   KVD+S+VAPNTTMSLSPW LEEDPH PKD   P AP  S+VKV DP+ RG+     
Sbjct: 68  TANHKVDDSNVAPNTTMSLSPWKLEEDPHGPKD---PRAPHISEVKVHDPSNRGSEEAAG 127

Query: 198 -SQIFDSFAKMKVNEEHEPNRTGLSSGIKQ--GGEES-SYTQKISGVGCAVSGKAVAAKD 257
            SQ+FDSFAKMKV+++ +PNRTG   G+ Q  GGE+  +Y QKIS VG AVSGKAVAAKD
Sbjct: 128 KSQVFDSFAKMKVDDK-KPNRTGSPHGLDQEHGGEDHINYAQKISAVGSAVSGKAVAAKD 187

Query: 258 YVASKLGYGETTEDNTNTNPKSCSPLEYGKKIALTVTEKLKPGEEDKALSEVISEAINRR 317
           +VASKLGYGET E  T     S SPLEYGKKIALTVTEKLKPGEED+ALSEVISEA+NRR
Sbjct: 188 FVASKLGYGETKEGTTADKSSSSSPLEYGKKIALTVTEKLKPGEEDRALSEVISEALNRR 247

Query: 318 KEEVVKVGESAFRQP--KGKVTESEGLTRRLGRED-----------SAPATGWSVVDKVK 377
           K+EVVKVGESAF +P  KG VTESE LTRRLG ED           +A ATG SVV  VK
Sbjct: 248 KDEVVKVGESAFGRPPSKGVVTESEELTRRLGEEDKEATEKSSVASAAAATGRSVVGMVK 307

Query: 378 DTVGTWLGKAGDQSSPSQQSVGTSQGVEGFMDSGRQRRQEPEPEPEPEPEPEPEPGGTEA 421
           DTVG+WLGKAG+QS+PSQQS+GTSQGVEGF+D    RRQ              E GGT A
Sbjct: 308 DTVGSWLGKAGEQSAPSQQSLGTSQGVEGFVDDSSSRRQ-------------AEHGGTGA 359

BLAST of Lag0030660 vs. ExPASy TrEMBL
Match: A0A0A0LIE7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G914030 PE=4 SV=1)

HSP 1 Score: 373.2 bits (957), Expect = 1.4e-99
Identity = 234/363 (64.46%), Postives = 269/363 (74.10%), Query Frame = 0

Query: 84  MRSAAVAGQGKHQDVGIGMSTTTHNHNQAAP--RETTTRPSAVDTAFTYVNAPTT--KVD 143
           MRS AVAG+G+HQDVGIG  TT   HN+  P  RE T+RPSAVDT FT ++  T   KVD
Sbjct: 1   MRS-AVAGKGQHQDVGIGTGTTRMMHNEPPPARREITSRPSAVDTGFTSIHNTTANYKVD 60

Query: 144 NSSVAPNTTMSLSPWSLEEDPHAPKDKTHPNAPPNSQVKVSDPARRGN------SQIFDS 203
           +S+VAPNTTMSLSPW LE+DPHAPKD   P+AP  S+VKV DP+ RG+      SQ+FDS
Sbjct: 61  DSNVAPNTTMSLSPWKLEDDPHAPKD---PHAPHISEVKVHDPSNRGSEEAAGRSQVFDS 120

Query: 204 FAKMKVNEEHEPNRTGLSSGI--KQGGE-ESSYTQKISGVGCAVSGKAVAAKDYVASKLG 263
           FA+MKV+++ EPNRTG   G+  +QGGE +++Y QKIS VG AVSGKAVAAKD+VASKLG
Sbjct: 121 FARMKVDDK-EPNRTGSPRGLVQEQGGEDQTNYAQKISAVGSAVSGKAVAAKDFVASKLG 180

Query: 264 YGETTEDNTNTNPKSCSPLEYGKKIALTVTEKLKPGEEDKALSEVISEAINRRKEEVVKV 323
           YGETTE+ T     S SPLEYGKKIALTVTEKLKPGEED+ALSEVISEA  RRK+EVVKV
Sbjct: 181 YGETTEETTTNKSSSSSPLEYGKKIALTVTEKLKPGEEDRALSEVISEAWTRRKDEVVKV 240

Query: 324 GESAFRQP--KGKVTESEGLTRRLGRED-----------SAPATGWSVVDKVKDTVGTWL 383
           GESAF +P  KG+VTESE LTRRLG+ED           +A ATG SVV  VKDTVG+WL
Sbjct: 241 GESAFGRPPSKGEVTESEELTRRLGKEDKEATEKSSVARAAAATGRSVVGMVKDTVGSWL 300

Query: 384 GKAGDQSSPSQQSVGTSQGVEGFMDSGRQRRQEPEPEPEPEPEPEPEPGGTEAEVRVLQE 421
           G AG+QS PSQQS+GTSQGVEGF+DS   RRQ              E GG  AEVR LQ 
Sbjct: 301 GNAGEQSVPSQQSLGTSQGVEGFVDSSSSRRQ-------------AEHGGKGAEVRRLQG 345

BLAST of Lag0030660 vs. ExPASy TrEMBL
Match: A0A6J1H2H6 (LOW QUALITY PROTEIN: low-temperature-induced 65 kDa protein-like OS=Cucurbita moschata OX=3662 GN=LOC111459097 PE=4 SV=1)

HSP 1 Score: 334.3 bits (856), Expect = 7.1e-88
Identity = 226/406 (55.67%), Postives = 263/406 (64.78%), Query Frame = 0

Query: 1   MDSQQINRHDH-----------ALHQSVGGEED---HEKKSVLKKVKAKAKKIKD----- 60
           M+SQQ   H H           ALH+SV GEED   HEKKSVLKKVKAKAKKIKD     
Sbjct: 1   MESQQTAPHRHHAHHHHDPHNVALHRSVEGEEDGQHHEKKSVLKKVKAKAKKIKDRSLSM 60

Query: 61  ---TITKHGHGHDHHDYEDEEDEDEDEGVVEDPQLQEAPLYEGAAMRSAAVAGQGKHQDV 120
              TITKHGHGHDHH+ ED EDE E  G V DP+LQ APLYEGA MRS A+AG+G+ QDV
Sbjct: 61  DMATITKHGHGHDHHEPEDHEDE-EGGGAVVDPELQGAPLYEGAVMRS-AMAGKGQQQDV 120

Query: 121 GIGMSTTTHNHNQAAPRETTTRPSAVDTAFTYVNAPTTKVDNSSVAPNTTMSLSPWSLEE 180
           GIG+ T  H+    APRET +RPSAV+                   PNTTMSLSPW LEE
Sbjct: 121 GIGLPTEVHDQPPPAPRETASRPSAVE-------------------PNTTMSLSPWRLEE 180

Query: 181 DPHAPKDKT-HPNAPPNSQVKVSDPARRGN-----SQIFDSFAKMKVNEEHEPNRTGLSS 240
            P APKDK  +P+ P NS+VK  DPA+RGN     SQ FDSFA+MK+N E    R GL+S
Sbjct: 181 GPQAPKDKAPYPHTPHNSEVKALDPAKRGNKETGDSQTFDSFARMKLNHEDVQTRPGLAS 240

Query: 241 GIKQGGEESSYTQKISGVGCAVSGKAVAAKDYVASKLGYGETTEDNTNTNPKSCSPLEYG 300
           GI QG + SS  +KISGVG  + GKA                 E+NT+TN  S SPLEYG
Sbjct: 241 GIDQGNQSSS-GEKISGVGSPMRGKA-----------------EENTDTN--SSSPLEYG 300

Query: 301 KKIALTVTEKLKPGEEDKALSEVISEAINRRKEEVVKVGESAFRQPKGKVTESEGLTRRL 360
           +KIAL VTEKLKPGEEDKALSEVISEA N  KE+VVK GE+   QPKGKVTESE LT+RL
Sbjct: 301 RKIALAVTEKLKPGEEDKALSEVISEAYNTSKEKVVKAGEN---QPKGKVTESEELTQRL 360

Query: 361 GREDS-------APATGW------SVVDKVKDTVGTWLGKAGDQSS 366
           G ED+       +PAT        ++ D V+D+VG+W+GK GD ++
Sbjct: 361 GGEDNNEGTQRYSPATSTTAATVKNMADMVRDSVGSWMGKGGDPNT 362

BLAST of Lag0030660 vs. ExPASy TrEMBL
Match: A0A6J1K662 (low-temperature-induced 65 kDa protein OS=Cucurbita maxima OX=3661 GN=LOC111491512 PE=4 SV=1)

HSP 1 Score: 333.6 bits (854), Expect = 1.2e-87
Identity = 217/379 (57.26%), Postives = 256/379 (67.55%), Query Frame = 0

Query: 9   HDHALHQSVGGEED---HEKKSVLKKVKAKAKKIKDTITKHGHGHDHHDYEDEEDEDEDE 68
           H+ AL++S  GEED   HEKKSVLKKVKAKAKKIKDTITKHGH HDHH+ ED E E+E  
Sbjct: 19  HNVALYRSAEGEEDGQHHEKKSVLKKVKAKAKKIKDTITKHGHAHDHHEPEDHE-EEEGG 78

Query: 69  GVVEDPQLQEAPLYEGAAMRSAAVAGQGKHQDVGIGMSTTTHNHNQAAPRETTTRPSAVD 128
           G V DP+LQ APLYEGAAMRS AVAG+G+ QDVGIG+ T  H+    APR T +RPSAV+
Sbjct: 79  GAVADPELQGAPLYEGAAMRS-AVAGKGQQQDVGIGLPTEVHDQPPPAPRGTASRPSAVE 138

Query: 129 TAFTYVNAPTTKVDNSSVAPNTTMSLSPWSLEEDPHAPKDKT-HPNAPPNSQVKVSDPAR 188
                              PNTTMSLSPW LEE P AP+DK  +P+ P NS+VK  DPA+
Sbjct: 139 -------------------PNTTMSLSPWRLEEGPQAPRDKAPYPHTPHNSEVKALDPAK 198

Query: 189 RGN-----SQIFDSFAKMKVNEEHEPNRTGLSSGIKQGGEESSYTQKISGVGCAVSGKAV 248
           RGN     SQ FDSFA+MK+N E    R GL+SGI QG + SS  +KISG G  + GKA 
Sbjct: 199 RGNEKTGDSQTFDSFARMKLNNEDVQTRPGLASGIDQGNQSSS-GEKISGAGSPMLGKA- 258

Query: 249 AAKDYVASKLGYGETTEDNTNTNPKSCSPLEYGKKIALTVTEKLKPGEEDKALSEVISEA 308
                           E+NT+TN  S SPLEYG+KIAL VTEKLKPGEEDKALSEVISEA
Sbjct: 259 ----------------EENTDTN--SSSPLEYGRKIALAVTEKLKPGEEDKALSEVISEA 318

Query: 309 INRRKEEVVKVGESAFRQPKGKVTESEGLTRRLGREDS-------APATGW------SVV 366
            N  KE+VVK GE+   QPKGKVTESE LT+RLGRED+       +PAT        ++ 
Sbjct: 319 YNSSKEKVVKAGEN---QPKGKVTESEELTQRLGREDNNEGTQRYSPATSTTAATVKNMA 353

BLAST of Lag0030660 vs. TAIR 10
Match: AT5G52300.1 (CAP160 protein )

HSP 1 Score: 85.5 bits (210), Expect = 1.1e-16
Identity = 156/587 (26.58%), Postives = 221/587 (37.65%), Query Frame = 0

Query: 20  EEDHEK--KSVLKKVKAKAKKIKDTITKHGHGHDHHDYEDEEDEDEDEGVVEDPQLQEAP 79
           EE HEK    VLKKVK KAKKIK+++TKHG+GHD HD ED++DE ++    +DP++  AP
Sbjct: 26  EEHHEKGASKVLKKVKEKAKKIKNSLTKHGNGHD-HDVEDDDDEYDE----QDPEVHGAP 85

Query: 80  LYEGAAMRSAAVAGQGKH---------------------------------------QDV 139
           +YE +A+R   V G+ K                                        QD 
Sbjct: 86  VYESSAVR-GGVTGKPKSLSHAGETNVPASEEIVPPGTKVFPVVSSDHTKPIEPVSLQDT 145

Query: 140 GIGMST------TTHNHNQAAPRETTTR-PSAV-------DTAFTYVNAPTTKVDNSSVA 199
             G         TT   +  A RE  T  P  V       ++   +     T V   S  
Sbjct: 146 SYGHEALADPVRTTETSDWEAKREAPTHYPLGVSEFSDRGESREAHQEPLNTPVSLLSAT 205

Query: 200 PNTTMSLSPWS------------------LEEDPHAP-KDKTHPNAPPNSQVKVSDPARR 259
            + T + +P                    LEEDP AP     + +   N Q KV+DP  +
Sbjct: 206 EDVTRTFAPGGEDDYLGGQRKVNVETPKRLEEDPAAPGGGSDYLSGVSNYQSKVTDPTHK 265

Query: 260 GNS----QIFDSFAKMKVNEE------------------HE-------------PNRTG- 319
           G      +I +S  +MKV +E                  HE             P R G 
Sbjct: 266 GGEAGVPEIAESLGRMKVTDESPDQKSRQGREEDFPTRSHEFDLKKESDINKNSPARFGG 325

Query: 320 -----------------LSSGI---------------------KQGGEE----------- 379
                            + SG+                     +   EE           
Sbjct: 326 ESKAGMEEDFPTRGDVKVESGLGRDLPTGTHDQFSPELSRPKERDDSEETKDESTHETKP 385

Query: 380 SSYTQKISGVGCAVSGKAVAAKDYVASKLGY-GETTEDNTNTNPKSCSP---LEYGKKIA 385
           S+YT++++    A++ KA+AAK+ VASKLGY GE     + +  K  +P     YG+K+A
Sbjct: 386 STYTEQLASATSAITNKAIAAKNVVASKLGYTGENGGGQSESPVKDETPRSVTAYGQKVA 445

BLAST of Lag0030660 vs. TAIR 10
Match: AT5G52300.2 (CAP160 protein )

HSP 1 Score: 85.5 bits (210), Expect = 1.1e-16
Identity = 156/587 (26.58%), Postives = 221/587 (37.65%), Query Frame = 0

Query: 20  EEDHEK--KSVLKKVKAKAKKIKDTITKHGHGHDHHDYEDEEDEDEDEGVVEDPQLQEAP 79
           EE HEK    VLKKVK KAKKIK+++TKHG+GHD HD ED++DE ++    +DP++  AP
Sbjct: 25  EEHHEKGASKVLKKVKEKAKKIKNSLTKHGNGHD-HDVEDDDDEYDE----QDPEVHGAP 84

Query: 80  LYEGAAMRSAAVAGQGKH---------------------------------------QDV 139
           +YE +A+R   V G+ K                                        QD 
Sbjct: 85  VYESSAVR-GGVTGKPKSLSHAGETNVPASEEIVPPGTKVFPVVSSDHTKPIEPVSLQDT 144

Query: 140 GIGMST------TTHNHNQAAPRETTTR-PSAV-------DTAFTYVNAPTTKVDNSSVA 199
             G         TT   +  A RE  T  P  V       ++   +     T V   S  
Sbjct: 145 SYGHEALADPVRTTETSDWEAKREAPTHYPLGVSEFSDRGESREAHQEPLNTPVSLLSAT 204

Query: 200 PNTTMSLSPWS------------------LEEDPHAP-KDKTHPNAPPNSQVKVSDPARR 259
            + T + +P                    LEEDP AP     + +   N Q KV+DP  +
Sbjct: 205 EDVTRTFAPGGEDDYLGGQRKVNVETPKRLEEDPAAPGGGSDYLSGVSNYQSKVTDPTHK 264

Query: 260 GNS----QIFDSFAKMKVNEE------------------HE-------------PNRTG- 319
           G      +I +S  +MKV +E                  HE             P R G 
Sbjct: 265 GGEAGVPEIAESLGRMKVTDESPDQKSRQGREEDFPTRSHEFDLKKESDINKNSPARFGG 324

Query: 320 -----------------LSSGI---------------------KQGGEE----------- 379
                            + SG+                     +   EE           
Sbjct: 325 ESKAGMEEDFPTRGDVKVESGLGRDLPTGTHDQFSPELSRPKERDDSEETKDESTHETKP 384

Query: 380 SSYTQKISGVGCAVSGKAVAAKDYVASKLGY-GETTEDNTNTNPKSCSP---LEYGKKIA 385
           S+YT++++    A++ KA+AAK+ VASKLGY GE     + +  K  +P     YG+K+A
Sbjct: 385 STYTEQLASATSAITNKAIAAKNVVASKLGYTGENGGGQSESPVKDETPRSVTAYGQKVA 444

BLAST of Lag0030660 vs. TAIR 10
Match: AT4G25580.1 (CAP160 protein )

HSP 1 Score: 48.9 bits (115), Expect = 1.2e-05
Identity = 153/608 (25.16%), Postives = 209/608 (34.38%), Query Frame = 0

Query: 1   MDSQQINRHDHALHQSV-------GGEEDHEKK---SVLKKVKAKAKKIKDTITKHGHGH 60
           MDSQ   +  H  HQ+          EE H +K    VLKKVK KAKKIK+ +TKHGHGH
Sbjct: 1   MDSQAQLQRTHGHHQAQEPIRIHHPEEEGHHEKGPSKVLKKVKEKAKKIKNVLTKHGHGH 60

Query: 61  DH---------HDYEDEEDEDEDEGVVEDPQLQEAPLYEGAA---MRSAAVAGQGKHQDV 120
           +H         HD + E+DED+      D QL       G A   ++   V    K   V
Sbjct: 61  EHDRGEHIPDDHDLDQEDDEDD----YNDNQLHGGAPARGKAHIPVKEEIVPPGSKAFPV 120

Query: 121 GIGMSTTTHNHNQAAPRETTTRP---SAVDTAFTYVNAPTTKVDNSSVA----------- 180
                T      +    E  +RP   S V        APT    N+ V+           
Sbjct: 121 VSSSHTKPSEPVRGVGHEAMSRPIKHSGVPEKEERRGAPTLTPHNTPVSLLSATEDVTRT 180

Query: 181 --PNTTMSLS--------PWSLEEDPHAP------------------------------- 240
             P+   S +        P  L++DP AP                               
Sbjct: 181 FVPDEHKSRNQSKVNIDRPKGLDQDPAAPGSHGGLSNYQSKVTDPIGKSTGEIGAASTVS 240

Query: 241 -----------KDKTH-----PNAP------PNSQVKVSDPARRGNSQIFDSFAKMKVNE 300
                      + K+H     P A        + Q KV+DP  +G  +     A  + NE
Sbjct: 241 AIGRLGLGTDLQKKSHGLDTKPTAQGSHGGMSSYQSKVTDPTGKGAGEQPSVAAFGRRNE 300

Query: 301 EHEPNRTGL--------------------------------------------------- 360
             E +  GL                                                   
Sbjct: 301 TGEDDHMGLGRDLREKSHGFDTKSRPEMGKHIPAGDYGSRLGKESPERSDEFDLGKDLPT 360

Query: 361 ------------SSGIKQGGE-----ESSYTQKISGVGCAVSGKAVAAKDYVASKLGY-G 377
                       S G ++G E     +SSYT KIS     V+ KAVAAK+ VASKLGY G
Sbjct: 361 RTQGIQNPQGFDSRGQRRGEEMHQPNQSSYTDKISLATSVVADKAVAAKNAVASKLGYSG 420