Lag0030649 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0030649
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	Reverse transcriptase domain-containing protein
Location	chr11: 126758 .. 127105 (+)
RNA-Seq Expression	Lag0030649
Synteny	Lag0030649

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCGTGTGTAGAATTGGTTTCCTTCTCAGTTCTAGTTAATGGGAACCAACAGGAGAGCTTTAAGCCATATAGGGGTATTAGGCAGGGCGACCCTTTATCGCCATATTTGTTTCTCATTTGCGCTGAAGGATTCACGACGTTACTTAACAGGGAAGAATCTCTCTCTAACTTGTCTGGCATTCGTTTCAATAAGAATAGTCCCTCACTTTCTCATCTTTTCTTTGCAGATGATAGCCTGGTGTTCCTAAAGGCCTCGAGCAAGGAGTGCAATACACTGAAGGAGGTGCTGAAAGTCTACGAAGAAGCGACTGCCCAGCGATTAATCTTGAGAAGTCTGAATGTTTGA

mRNA sequence

ATGTCGTGTGTAGAATTGGTTTCCTTCTCAGTTCTAGTTAATGGGAACCAACAGGAGAGCTTTAAGCCATATAGGGGTATTAGGCAGGGCGACCCTTTATCGCCATATTTGTTTCTCATTTGCGCTGAAGGATTCACGACGTTACTTAACAGGGAAGAATCTCTCTCTAACTTGTCTGGCATTCGTTTCAATAAGAATAGTCCCTCACTTTCTCATCTTTTCTTTGCAGATGATAGCCTGGTGTTCCTAAAGGCCTCGAGCAAGGAGTGCAATACACTGAAGGAGGTGCTGAAAGTCTACGAAGAAGCGACTGCCCAGCGATTAATCTTGAGAAGTCTGAATGTTTGA

Coding sequence (CDS)

ATGTCGTGTGTAGAATTGGTTTCCTTCTCAGTTCTAGTTAATGGGAACCAACAGGAGAGCTTTAAGCCATATAGGGGTATTAGGCAGGGCGACCCTTTATCGCCATATTTGTTTCTCATTTGCGCTGAAGGATTCACGACGTTACTTAACAGGGAAGAATCTCTCTCTAACTTGTCTGGCATTCGTTTCAATAAGAATAGTCCCTCACTTTCTCATCTTTTCTTTGCAGATGATAGCCTGGTGTTCCTAAAGGCCTCGAGCAAGGAGTGCAATACACTGAAGGAGGTGCTGAAAGTCTACGAAGAAGCGACTGCCCAGCGATTAATCTTGAGAAGTCTGAATGTTTGA

Protein sequence

MSCVELVSFSVLVNGNQQESFKPYRGIRQGDPLSPYLFLICAEGFTTLLNREESLSNLSGIRFNKNSPSLSHLFFADDSLVFLKASSKECNTLKEVLKVYEEATAQRLILRSLNV

Homology

[Close]

BLAST of Lag0030649 vs. NCBI nr
Match: XP_030948949.1 (uncharacterized protein LOC115972854 [Quercus lobata])

HSP 1 Score: 131.0 bits (328), Expect = 6.8e-27
Identity = 62/108 (57.41%), Postives = 78/108 (72.22%), Query Frame = 0


Query: 1   MSCVELVSFSVLVNGNQQESFKPYRGIRQGDPLSPYLFLICAEGFTTLLNREESLSNLSG 60
           M C+  VS+S+LVNG+  ESF P RG+RQGDPLSPYLFLIC+E    LLN+E    ++ G
Sbjct: 56  MECITTVSYSILVNGDPSESFLPSRGLRQGDPLSPYLFLICSERLHGLLNKEAEEGHIRG 115

Query: 61  IRFNKNSPSLSHLFFADDSLVFLKASSKECNTLKEVLKVYEEATAQRL 109
           +   K  P L+HLFFADDSLVF KAS  EC  ++ VL VYE+A+ Q+L
Sbjct: 116 VSICKKGPRLTHLFFADDSLVFCKASIAECQNIQSVLNVYEKASGQKL 163

[Close]

BLAST of Lag0030649 vs. NCBI nr
Match: OMO90013.1 (reverse transcriptase [Corchorus capsularis])

HSP 1 Score: 129.8 bits (325), Expect = 1.5e-26
Identity = 58/108 (53.70%), Postives = 81/108 (75.00%), Query Frame = 0


Query: 1   MSCVELVSFSVLVNGNQQESFKPYRGIRQGDPLSPYLFLICAEGFTTLLNREESLSNLSG 60
           M CV  VS+S+LVNG Q     P RGIRQGDPLSPYLFL+C EGF++LL   E + ++ G
Sbjct: 875 MQCVRTVSYSILVNGVQTRRIVPSRGIRQGDPLSPYLFLLCMEGFSSLLQHAERIGDIHG 934

Query: 61  IRFNKNSPSLSHLFFADDSLVFLKASSKECNTLKEVLKVYEEATAQRL 109
           +   +N+P +SHLFFADDSL+FL+AS  EC+ +K +L+++E A+ Q++
Sbjct: 935 VEVARNAPCISHLFFADDSLLFLRASLGECDAIKNLLRIFELASGQQI 982

[Close]

BLAST of Lag0030649 vs. NCBI nr
Match: XP_027185015.1 (uncharacterized protein LOC113783152 [Coffea eugenioides])

HSP 1 Score: 129.4 bits (324), Expect = 2.0e-26
Identity = 60/115 (52.17%), Postives = 84/115 (73.04%), Query Frame = 0


Query: 1   MSCVELVSFSVLVNGNQQESFKPYRGIRQGDPLSPYLFLICAEGFTTLLNREESLSNLSG 60
           M C+  VSFS  +NG  +E   P RGIRQGDPLSPYLFLIC+EGF++LL R     N++G
Sbjct: 554 MKCISTVSFSFNINGECKEYIVPERGIRQGDPLSPYLFLICSEGFSSLLKRATQTRNMAG 613

Query: 61  IRFNKNSPSLSHLFFADDSLVFLKASSKECNTLKEVLKVYEEATAQRLILRSLNV 116
           ++ +++ PS++HLFFADDSL+F KA+S+E   LK +L+VYE+ + Q + L   +V
Sbjct: 614 MKISRHGPSITHLFFADDSLIFCKANSQEAKELKRILEVYEQESGQMVNLDKSSV 668

[Close]

BLAST of Lag0030649 vs. NCBI nr
Match: XP_027086414.1 (uncharacterized protein LOC113708145 [Coffea arabica])

HSP 1 Score: 128.3 bits (321), Expect = 4.4e-26
Identity = 60/115 (52.17%), Postives = 84/115 (73.04%), Query Frame = 0


Query: 1   MSCVELVSFSVLVNGNQQESFKPYRGIRQGDPLSPYLFLICAEGFTTLLNREESLSNLSG 60
           M C+  VSFS  +NG  +E   P RGIRQGDPLSPYLFLIC+EGF++LL R     N++G
Sbjct: 12  MKCISTVSFSFNINGECKEYIVPERGIRQGDPLSPYLFLICSEGFSSLLKRATQTRNMAG 71

Query: 61  IRFNKNSPSLSHLFFADDSLVFLKASSKECNTLKEVLKVYEEATAQRLILRSLNV 116
           ++ +++ PS++HLFFADDSL+F KA+S+E   LK +L+VYE+ + Q + L   +V
Sbjct: 72  MKRSRHGPSITHLFFADDSLIFCKANSQEAKELKRILEVYEQESGQMVNLDKSSV 126

[Close]

BLAST of Lag0030649 vs. NCBI nr
Match: XP_023880426.1 (uncharacterized protein LOC111992797 [Quercus suber])

HSP 1 Score: 127.9 bits (320), Expect = 5.7e-26
Identity = 57/108 (52.78%), Postives = 79/108 (73.15%), Query Frame = 0


Query: 1   MSCVELVSFSVLVNGNQQESFKPYRGIRQGDPLSPYLFLICAEGFTTLLNREESLSNLSG 60
           M C+  VS+SV++NG    +  P RG+RQGDPLS YLFL+CAEGF+ L+N   S + L+G
Sbjct: 37  MKCISSVSYSVIINGTTYGNITPNRGLRQGDPLSLYLFLLCAEGFSALINEVASNNQLNG 96

Query: 61  IRFNKNSPSLSHLFFADDSLVFLKASSKECNTLKEVLKVYEEATAQRL 109
           I   + +P +SHLFFADDS++F KA+  ECN LKE+L +YE A+ Q++
Sbjct: 97  ISICRGAPKVSHLFFADDSIIFCKANGNECNKLKEILGLYESASGQKI 144

[Close]

BLAST of Lag0030649 vs. ExPASy Swiss-Prot
Match: P92555 (Uncharacterized mitochondrial protein AtMg01250 OS=Arabidopsis thaliana OX=3702 GN=AtMg01250 PE=4 SV=1)

HSP 1 Score: 77.8 bits (190), Expect = 8.9e-14
Identity = 34/68 (50.00%), Postives = 45/68 (66.18%), Query Frame = 0


Query: 12 LVNGNQQESFKPYRGIRQGDPLSPYLFLICAEGFTTLLNREESLSNLSGIRFNKNSPSLS 71
          ++NG  Q    P RG+RQGDPLSPYLF++C E  + L  R +    L GIR + NSP ++
Sbjct: 13 IINGAPQGLVTPSRGLRQGDPLSPYLFILCTEVLSGLCRRAQEQGRLPGIRVSNNSPRIN 72

Query: 72 HLFFADDS 80
          HL FADD+
Sbjct: 73 HLLFADDT 80

[Close]

BLAST of Lag0030649 vs. ExPASy Swiss-Prot
Match: P11369 (LINE-1 retrotransposable element ORF2 protein OS=Mus musculus OX=10090 GN=Pol PE=1 SV=2)

HSP 1 Score: 46.6 bits (109), Expect = 2.2e-04
Identity = 28/99 (28.28%), Postives = 47/99 (47.47%), Query Frame = 0


Query: 10  SVLVNGNQQESFKPYRGIRQGDPLSPYLFLICAEGFTTLLNREESLSNLSGIRFNKNSPS 69
           ++ VNG + E+     G RQG PLSPYLF I  E     + +++    + GI+  K    
Sbjct: 647 NIKVNGEKLEAIPLKSGTRQGCPLSPYLFNIVLEVLARAIRQQK---EIKGIQIGKEEVK 706

Query: 70  LSHLFFADDSLVFLKASSKECNTLKEVLKVYEEATAQRL 109
           +S L  ADD +V++         L  ++  + E    ++
Sbjct: 707 ISLL--ADDMIVYISDPKNSTRELLNLINSFGEVVGYKI 740

The following BLAST results are available for this feature:

BLAST of Lag0030649 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_030948949.1	6.8e-27	57.41	uncharacterized protein LOC115972854 [Quercus lobata]	[more]
OMO90013.1	1.5e-26	53.70	reverse transcriptase [Corchorus capsularis]	[more]
XP_027185015.1	2.0e-26	52.17	uncharacterized protein LOC113783152 [Coffea eugenioides]	[more]
XP_027086414.1	4.4e-26	52.17	uncharacterized protein LOC113708145 [Coffea arabica]	[more]
XP_023880426.1	5.7e-26	52.78	uncharacterized protein LOC111992797 [Quercus suber]	[more]

BLAST of Lag0030649 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P92555	8.9e-14	50.00	Uncharacterized mitochondrial protein AtMg01250 OS=Arabidopsis thaliana OX=3702 ...	[more]
P11369	2.2e-04	28.28	LINE-1 retrotransposable element ORF2 protein OS=Mus musculus OX=10090 GN=Pol PE...	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 83..103
None	No IPR available	PANTHER	PTHR33116:SF45	SUBFAMILY NOT NAMED	coord: 1..110
None	No IPR available	PANTHER	PTHR33116	REVERSE TRANSCRIPTASE ZINC-BINDING DOMAIN-CONTAINING PROTEIN-RELATED-RELATED	coord: 1..110
IPR000477	Reverse transcriptase domain	PFAM	PF00078	RVT_1	coord: 3..103 e-value: 1.2E-12 score: 47.8
IPR043502	DNA/RNA polymerase superfamily	SUPERFAMILY	56672	DNA/RNA polymerases	coord: 12..98

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0030649.1	Lag0030649.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0090304	nucleic acid metabolic process
molecular_function	GO:0140640	catalytic activity, acting on a nucleic acid