Lag0030473 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0030473
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	NAD(P)H-quinone oxidoreductase subunit T, chloroplastic
Location	chr8: 47531050 .. 47531867 (+)
RNA-Seq Expression	Lag0030473
Synteny	Lag0030473

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCCATGGCCTCCCCTCCTATTCCGAGTTCTCTCTTCTTGCGCAGAAGCTTCCGCTTCCACTCGCCTGCTCGCATATTCAATATCAATACATGGCGGGTCTATGCATCTCAACCTCCTAGTCCTAACAACCGCCCAAGGCCTCCACCGGGCGTCGACACAAGAATTCATTGGCAAAATGAAGAAGAGGGTTGGATTGGAGGCCCTACACATTCCCATCCAGACCAGAGGAACGAGAATAATAATATCTTGGCTCCAAGTTTGGCCGATTTGCTCAATAACTCATCCGATTCTCACTACCAGTACGTGAGTAGCTATGTGCTTCATCAATTACAATTTGAATTTGAATTTGAATTTGATTTGTATTGTGTATATATGCGCTGCGCATGTGCAGGTTCTTAGGAGTGAGTGCAGAGGCTGACCTAGAAGAGATAAAAAGTGCGTATCGGAGATTATCAAAAGAATACCATCCCGACACCACCTCTCTCCCTCTAAAAGCTGCCTCCGACAAATTCATGAAATTAAAACAAGTGTACGAGGTCCTCAGCAACGAAGAAAGCCGCAAGTTCTACGACTGGACGCTTGCTCAAGAAGAGGCGAGCCGCCAAGCTAGCAAATTGAGGATCAAGTTGGAAGATCCTTACGAGCAACAGTTGCAAAACTGGAAATCCACTCCGGACATGGTCGATCGCCTTGCAGGAAGGAACATGGACTTGAGTGATCAGGCCACCACAGCTCTTACCTTGGATATCCTTATCATTCTCTTTGCTATTTGCTGCATTGTATATGTCCTACTTTTTAAAGAACCATATTATTAG

mRNA sequence

ATGGCCATGGCCTCCCCTCCTATTCCGAGTTCTCTCTTCTTGCGCAGAAGCTTCCGCTTCCACTCGCCTGCTCGCATATTCAATATCAATACATGGCGGGTCTATGCATCTCAACCTCCTAGTCCTAACAACCGCCCAAGGCCTCCACCGGGCGTCGACACAAGAATTCATTGGCAAAATGAAGAAGAGGGTTGGATTGGAGGCCCTACACATTCCCATCCAGACCAGAGGAACGAGAATAATAATATCTTGGCTCCAAGTTTGGCCGATTTGCTCAATAACTCATCCGATTCTCACTACCAGTTCTTAGGAGTGAGTGCAGAGGCTGACCTAGAAGAGATAAAAAGTGCGTATCGGAGATTATCAAAAGAATACCATCCCGACACCACCTCTCTCCCTCTAAAAGCTGCCTCCGACAAATTCATGAAATTAAAACAAGTGTACGAGGTCCTCAGCAACGAAGAAAGCCGCAAGTTCTACGACTGGACGCTTGCTCAAGAAGAGGCGAGCCGCCAAGCTAGCAAATTGAGGATCAAGTTGGAAGATCCTTACGAGCAACAGTTGCAAAACTGGAAATCCACTCCGGACATGGTCGATCGCCTTGCAGGAAGGAACATGGACTTGAGTGATCAGGCCACCACAGCTCTTACCTTGGATATCCTTATCATTCTCTTTGCTATTTGCTGCATTGTATATGTCCTACTTTTTAAAGAACCATATTATTAG

Coding sequence (CDS)

ATGGCCATGGCCTCCCCTCCTATTCCGAGTTCTCTCTTCTTGCGCAGAAGCTTCCGCTTCCACTCGCCTGCTCGCATATTCAATATCAATACATGGCGGGTCTATGCATCTCAACCTCCTAGTCCTAACAACCGCCCAAGGCCTCCACCGGGCGTCGACACAAGAATTCATTGGCAAAATGAAGAAGAGGGTTGGATTGGAGGCCCTACACATTCCCATCCAGACCAGAGGAACGAGAATAATAATATCTTGGCTCCAAGTTTGGCCGATTTGCTCAATAACTCATCCGATTCTCACTACCAGTTCTTAGGAGTGAGTGCAGAGGCTGACCTAGAAGAGATAAAAAGTGCGTATCGGAGATTATCAAAAGAATACCATCCCGACACCACCTCTCTCCCTCTAAAAGCTGCCTCCGACAAATTCATGAAATTAAAACAAGTGTACGAGGTCCTCAGCAACGAAGAAAGCCGCAAGTTCTACGACTGGACGCTTGCTCAAGAAGAGGCGAGCCGCCAAGCTAGCAAATTGAGGATCAAGTTGGAAGATCCTTACGAGCAACAGTTGCAAAACTGGAAATCCACTCCGGACATGGTCGATCGCCTTGCAGGAAGGAACATGGACTTGAGTGATCAGGCCACCACAGCTCTTACCTTGGATATCCTTATCATTCTCTTTGCTATTTGCTGCATTGTATATGTCCTACTTTTTAAAGAACCATATTATTAG

Protein sequence

MAMASPPIPSSLFLRRSFRFHSPARIFNINTWRVYASQPPSPNNRPRPPPGVDTRIHWQNEEEGWIGGPTHSHPDQRNENNNILAPSLADLLNNSSDSHYQFLGVSAEADLEEIKSAYRRLSKEYHPDTTSLPLKAASDKFMKLKQVYEVLSNEESRKFYDWTLAQEEASRQASKLRIKLEDPYEQQLQNWKSTPDMVDRLAGRNMDLSDQATTALTLDILIILFAICCIVYVLLFKEPYY

Homology

[Close]

BLAST of Lag0030473 vs. NCBI nr
Match: XP_022990181.1 (NAD(P)H-quinone oxidoreductase subunit T, chloroplastic [Cucurbita maxima])

HSP 1 Score: 397.9 bits (1021), Expect = 6.2e-107
Identity = 213/243 (87.65%), Postives = 219/243 (90.12%), Query Frame = 0


Query: 1   MAMASPPIPSSLFLRRSFRFHSPARIFNINTWRVYASQPPSPNNRPRPPPGVDTRIHWQN 60
           MAMAS PIP+SLFL R+  FHSPAR F   TW VYASQP  PNNR RPPPGVDTRIHWQN
Sbjct: 1   MAMAS-PIPNSLFLNRT--FHSPARTF--RTWPVYASQP--PNNRQRPPPGVDTRIHWQN 60

Query: 61  EEEGWIGGPT--HSHPDQRNENNNILAPSLADLLNNSSDSHYQFLGVSAEADLEEIKSAY 120
           +EEGWIGGPT   S  DQ+ +NNN L PS ADLLNNSSDSHYQFLGV AEADLEEIKSAY
Sbjct: 61  DEEGWIGGPTPSQSRRDQKEQNNNALGPSFADLLNNSSDSHYQFLGVGAEADLEEIKSAY 120

Query: 121 RRLSKEYHPDTTSLPLKAASDKFMKLKQVYEVLSNEESRKFYDWTLAQEEASRQASKLRI 180
           RRLSKEYHPDTTSLPLKAASDKFMKLKQVYEVLSNEESRKFYDWTLAQEEASRQA KLRI
Sbjct: 121 RRLSKEYHPDTTSLPLKAASDKFMKLKQVYEVLSNEESRKFYDWTLAQEEASRQADKLRI 180

Query: 181 KLEDPYEQQLQNWKSTPDMVDRLAGRNMDLSDQATTALTLDILIILFAICCIVYVLLFKE 240
           KLEDPYEQ+LQNWKSTPDMVDRLAGRNMDLSDQATTALTLDILIILFAI CIVYVLLFKE
Sbjct: 181 KLEDPYEQELQNWKSTPDMVDRLAGRNMDLSDQATTALTLDILIILFAIGCIVYVLLFKE 236

Query: 241 PYY 242
           PYY
Sbjct: 241 PYY 236

[Close]

BLAST of Lag0030473 vs. NCBI nr
Match: KAG7033427.1 (NAD(P)H-quinone oxidoreductase subunit T, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 396.4 bits (1017), Expect = 1.8e-106
Identity = 213/247 (86.23%), Postives = 219/247 (88.66%), Query Frame = 0


Query: 1   MAMASPPIPSSLFLRRSFRFHSPARIFNINTWRVYASQPPSPNNRPRPPPGVDTRIHWQN 60
           +AMASP IP+SLFL R+  FHSPAR F   TW VYASQP  PNNR RPPPGVDTRIHWQN
Sbjct: 242 LAMASPSIPNSLFLNRT--FHSPARTF--RTWPVYASQP--PNNRQRPPPGVDTRIHWQN 301

Query: 61  EEEGWIGGPT--HSHPDQR----NENNNILAPSLADLLNNSSDSHYQFLGVSAEADLEEI 120
           +EEGWIGGPT   S  DQ+    N NNN L PSL DLLNNSSDSHYQFLGV AEADLEEI
Sbjct: 302 DEEGWIGGPTPSQSRRDQKEQNNNNNNNALGPSLTDLLNNSSDSHYQFLGVGAEADLEEI 361

Query: 121 KSAYRRLSKEYHPDTTSLPLKAASDKFMKLKQVYEVLSNEESRKFYDWTLAQEEASRQAS 180
           KSAYRRLSKEYHPDTTSLPLKAASDKFMKLKQVYEVLSNEESRKFYDWTLAQEEASRQA 
Sbjct: 362 KSAYRRLSKEYHPDTTSLPLKAASDKFMKLKQVYEVLSNEESRKFYDWTLAQEEASRQAD 421

Query: 181 KLRIKLEDPYEQQLQNWKSTPDMVDRLAGRNMDLSDQATTALTLDILIILFAICCIVYVL 240
           KLRIKLEDPYEQ+LQNWKSTPDMVDRLAGRNMDLSDQATTALTLDILIILFAI CIVYVL
Sbjct: 422 KLRIKLEDPYEQELQNWKSTPDMVDRLAGRNMDLSDQATTALTLDILIILFAIGCIVYVL 481

Query: 241 LFKEPYY 242
           LFKEPYY
Sbjct: 482 LFKEPYY 482

[Close]

BLAST of Lag0030473 vs. NCBI nr
Match: KAG6602740.1 (NAD(P)H-quinone oxidoreductase subunit T, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 396.4 bits (1017), Expect = 1.8e-106
Identity = 213/247 (86.23%), Postives = 219/247 (88.66%), Query Frame = 0


Query: 1   MAMASPPIPSSLFLRRSFRFHSPARIFNINTWRVYASQPPSPNNRPRPPPGVDTRIHWQN 60
           +AMASP IP+SLFL R+  FHSPAR F   TW VYASQP  PNNR RPPPGVDTRIHWQN
Sbjct: 233 LAMASPSIPNSLFLNRT--FHSPARTF--RTWPVYASQP--PNNRQRPPPGVDTRIHWQN 292

Query: 61  EEEGWIGGPT--HSHPDQR----NENNNILAPSLADLLNNSSDSHYQFLGVSAEADLEEI 120
           +EEGWIGGPT   S  DQ+    N NNN L PSL DLLNNSSDSHYQFLGV AEADLEEI
Sbjct: 293 DEEGWIGGPTPSQSRRDQKEQNNNNNNNALGPSLTDLLNNSSDSHYQFLGVGAEADLEEI 352

Query: 121 KSAYRRLSKEYHPDTTSLPLKAASDKFMKLKQVYEVLSNEESRKFYDWTLAQEEASRQAS 180
           KSAYRRLSKEYHPDTTSLPLKAASDKFMKLKQVYEVLSNEESRKFYDWTLAQEEASRQA 
Sbjct: 353 KSAYRRLSKEYHPDTTSLPLKAASDKFMKLKQVYEVLSNEESRKFYDWTLAQEEASRQAD 412

Query: 181 KLRIKLEDPYEQQLQNWKSTPDMVDRLAGRNMDLSDQATTALTLDILIILFAICCIVYVL 240
           KLRIKLEDPYEQ+LQNWKSTPDMVDRLAGRNMDLSDQATTALTLDILIILFAI CIVYVL
Sbjct: 413 KLRIKLEDPYEQELQNWKSTPDMVDRLAGRNMDLSDQATTALTLDILIILFAIGCIVYVL 472

Query: 241 LFKEPYY 242
           LFKEPYY
Sbjct: 473 LFKEPYY 473

[Close]

BLAST of Lag0030473 vs. NCBI nr
Match: XP_023521950.1 (NAD(P)H-quinone oxidoreductase subunit T, chloroplastic-like [Cucurbita pepo subsp. pepo] >XP_023553300.1 NAD(P)H-quinone oxidoreductase subunit T, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 395.6 bits (1015), Expect = 3.1e-106
Identity = 213/247 (86.23%), Postives = 220/247 (89.07%), Query Frame = 0


Query: 1   MAMASPPIPSSLFLRRSFRFHSPARIFNINTWRVYASQPPSPNNRPRPPPGVDTRIHWQN 60
           MAMASP IP+SLFL R+  FHSPAR F   TW VYASQP  PNNR RPPPGVDTRIHWQN
Sbjct: 1   MAMASPSIPNSLFLNRT--FHSPARTF--RTWPVYASQP--PNNRQRPPPGVDTRIHWQN 60

Query: 61  EEEGWIGG--PTHSHPDQR----NENNNILAPSLADLLNNSSDSHYQFLGVSAEADLEEI 120
           +EEGWIGG  P+ S  DQ+    N NNN L PSLADLLNNSSDSHYQFLGV AEADLEEI
Sbjct: 61  DEEGWIGGPRPSQSRRDQKEQNNNNNNNALGPSLADLLNNSSDSHYQFLGVGAEADLEEI 120

Query: 121 KSAYRRLSKEYHPDTTSLPLKAASDKFMKLKQVYEVLSNEESRKFYDWTLAQEEASRQAS 180
           KSAYRRLSKEYHPDTTSLPLKAASDKFMKLKQVYEVLSNEESRKFYDWTLAQEEASRQA 
Sbjct: 121 KSAYRRLSKEYHPDTTSLPLKAASDKFMKLKQVYEVLSNEESRKFYDWTLAQEEASRQAD 180

Query: 181 KLRIKLEDPYEQQLQNWKSTPDMVDRLAGRNMDLSDQATTALTLDILIILFAICCIVYVL 240
           KLRIKLEDPYEQ+LQ+WKSTPDMVDRLAGRNMDLSDQATTALTLDILIILFAI CIVYVL
Sbjct: 181 KLRIKLEDPYEQELQSWKSTPDMVDRLAGRNMDLSDQATTALTLDILIILFAIGCIVYVL 240

Query: 241 LFKEPYY 242
           LFKEPYY
Sbjct: 241 LFKEPYY 241

[Close]

BLAST of Lag0030473 vs. NCBI nr
Match: XP_022963850.1 (NAD(P)H-quinone oxidoreductase subunit T, chloroplastic [Cucurbita moschata])

HSP 1 Score: 394.4 bits (1012), Expect = 6.9e-106
Identity = 212/245 (86.53%), Postives = 217/245 (88.57%), Query Frame = 0


Query: 3   MASPPIPSSLFLRRSFRFHSPARIFNINTWRVYASQPPSPNNRPRPPPGVDTRIHWQNEE 62
           MASP IP+SLFL R+  FHSPAR F   TW VYASQP  PNNR RPPPGVDTRIHWQN+E
Sbjct: 1   MASPSIPNSLFLNRT--FHSPARTF--RTWPVYASQP--PNNRQRPPPGVDTRIHWQNDE 60

Query: 63  EGWIGGPT--HSHPDQR----NENNNILAPSLADLLNNSSDSHYQFLGVSAEADLEEIKS 122
           EGWIGGPT   S  DQ+    N NNN L PSL DLLNNSSDSHYQFLGV AEADLEEIKS
Sbjct: 61  EGWIGGPTPSQSRRDQKEQNNNNNNNALGPSLTDLLNNSSDSHYQFLGVGAEADLEEIKS 120

Query: 123 AYRRLSKEYHPDTTSLPLKAASDKFMKLKQVYEVLSNEESRKFYDWTLAQEEASRQASKL 182
           AYRRLSKEYHPDTTSLPLKAASDKFMKLKQVYEVLSNEESRKFYDWTLAQEEASRQA KL
Sbjct: 121 AYRRLSKEYHPDTTSLPLKAASDKFMKLKQVYEVLSNEESRKFYDWTLAQEEASRQADKL 180

Query: 183 RIKLEDPYEQQLQNWKSTPDMVDRLAGRNMDLSDQATTALTLDILIILFAICCIVYVLLF 242
           RIKLEDPYEQ+LQNWKSTPDMVDRLAGRNMDLSDQATTALTLDILIILFAI CIVYVLLF
Sbjct: 181 RIKLEDPYEQELQNWKSTPDMVDRLAGRNMDLSDQATTALTLDILIILFAIGCIVYVLLF 239

[Close]

BLAST of Lag0030473 vs. ExPASy Swiss-Prot
Match: Q9SMS0 (NAD(P)H-quinone oxidoreductase subunit T, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ndhT PE=1 SV=1)

HSP 1 Score: 272.7 bits (696), Expect = 4.0e-72
Identity = 138/210 (65.71%), Postives = 169/210 (80.48%), Query Frame = 0


Query: 33  RVYASQPPSPNNRPRPPPGVDTRIHWQNEEEGWIGGPTHSHPDQRNENNNILA-PSLADL 92
           R+YASQ   P    +P PGVDTRIHW++ +EGWIGG +        +  N+L+    A+L
Sbjct: 42  RIYASQ--GPTKPSKPSPGVDTRIHWESPDEGWIGGRSDPAKSVDEDKTNLLSDEKFAEL 101

Query: 93  LNNSSDSHYQFLGVSAEADLEEIKSAYRRLSKEYHPDTTSLPLKAASDKFMKLKQVYEVL 152
           + +S DSHYQFLGVS +ADLEEIKSAYRRLSKEYHPDTTSLPLK AS+KFMKL++VY VL
Sbjct: 102 IKDSFDSHYQFLGVSTDADLEEIKSAYRRLSKEYHPDTTSLPLKTASEKFMKLREVYNVL 161

Query: 153 SNEESRKFYDWTLAQEEASRQASKLRIKLEDPYEQQLQNWKSTPDMVDRLAGRNMDLSDQ 212
           S+EE+R+FYDWTLAQE ASRQA K+R+KLEDP EQ  + ++S PDMVDRL GRNM+LSDQ
Sbjct: 162 SDEETRRFYDWTLAQEVASRQAEKMRMKLEDPKEQDFRGYESIPDMVDRLGGRNMELSDQ 221

Query: 213 ATTALTLDILIILFAICCIVYVLLFKEPYY 242
           A TALT DILI+LFA+CCI +V++FK+P Y
Sbjct: 222 AMTALTFDILIVLFAVCCIAFVIVFKDPSY 249

[Close]

BLAST of Lag0030473 vs. ExPASy Swiss-Prot
Match: Q75VW3 (Chaperone protein DnaJ OS=Hydrogenobacter thermophilus (strain DSM 6534 / IAM 12695 / TK-6) OX=608538 GN=dnaJ PE=3 SV=1)

HSP 1 Score: 69.7 bits (169), Expect = 5.1e-11
Identity = 35/63 (55.56%), Postives = 47/63 (74.60%), Query Frame = 0


Query: 99  HYQFLGVSAEADLEEIKSAYRRLSKEYHPDTTSLPLKAASDKFMKLKQVYEVLSNEESRK 158
           +YQ LGVS +A  EEIK AYRRL+KEYHPD ++   + AS+KF ++ + Y +LS+EE RK
Sbjct: 6   YYQILGVSKDATAEEIKKAYRRLAKEYHPDISA--DENASEKFKEINEAYHILSDEERRK 65

Query: 159 FYD 162
            YD
Sbjct: 66  EYD 66

[Close]

BLAST of Lag0030473 vs. ExPASy Swiss-Prot
Match: Q5KWZ8 (Chaperone protein DnaJ OS=Geobacillus kaustophilus (strain HTA426) OX=235909 GN=dnaJ PE=3 SV=1)

HSP 1 Score: 68.6 bits (166), Expect = 1.1e-10
Identity = 33/63 (52.38%), Postives = 45/63 (71.43%), Query Frame = 0


Query: 99  HYQFLGVSAEADLEEIKSAYRRLSKEYHPDTTSLPLKAASDKFMKLKQVYEVLSNEESRK 158
           +Y+ LGVS  A  +EIK AYR+LSK+YHPD    P   A++KF ++K+ YEVLS++E R 
Sbjct: 6   YYEILGVSKNATKDEIKKAYRKLSKQYHPDVNKAP--DAAEKFKEIKEAYEVLSDDEKRA 65

Query: 159 FYD 162
            YD
Sbjct: 66  RYD 66

[Close]

BLAST of Lag0030473 vs. ExPASy Swiss-Prot
Match: Q45552 (Chaperone protein DnaJ OS=Geobacillus stearothermophilus OX=1422 GN=dnaJ PE=3 SV=1)

HSP 1 Score: 68.2 bits (165), Expect = 1.5e-10
Identity = 33/63 (52.38%), Postives = 45/63 (71.43%), Query Frame = 0


Query: 99  HYQFLGVSAEADLEEIKSAYRRLSKEYHPDTTSLPLKAASDKFMKLKQVYEVLSNEESRK 158
           +Y+ LGVS  A  EEIK AYR+LSK+YHPD    P   A++KF ++K+ YEVLS+++ R 
Sbjct: 6   YYEILGVSKNATKEEIKKAYRKLSKKYHPDVNKEP--DAAEKFKEIKEAYEVLSDDQKRA 65

Query: 159 FYD 162
            YD
Sbjct: 66  HYD 66

[Close]

BLAST of Lag0030473 vs. ExPASy Swiss-Prot
Match: C5D4U0 (Chaperone protein DnaJ OS=Geobacillus sp. (strain WCH70) OX=471223 GN=dnaJ PE=3 SV=1)

HSP 1 Score: 67.8 bits (164), Expect = 1.9e-10
Identity = 33/63 (52.38%), Postives = 45/63 (71.43%), Query Frame = 0


Query: 99  HYQFLGVSAEADLEEIKSAYRRLSKEYHPDTTSLPLKAASDKFMKLKQVYEVLSNEESRK 158
           +Y+ LGVS  A  EEIK AYR+LSK+YHPD    P   A++KF ++K+ YEVLS+++ R 
Sbjct: 6   YYEILGVSKNATKEEIKKAYRKLSKKYHPDINKEP--DAAEKFKEIKEAYEVLSDDQKRA 65

Query: 159 FYD 162
            YD
Sbjct: 66  HYD 66

[Close]

BLAST of Lag0030473 vs. ExPASy TrEMBL
Match: A0A6J1JM84 (NAD(P)H-quinone oxidoreductase subunit T, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111487152 PE=4 SV=1)

HSP 1 Score: 397.9 bits (1021), Expect = 3.0e-107
Identity = 213/243 (87.65%), Postives = 219/243 (90.12%), Query Frame = 0


Query: 1   MAMASPPIPSSLFLRRSFRFHSPARIFNINTWRVYASQPPSPNNRPRPPPGVDTRIHWQN 60
           MAMAS PIP+SLFL R+  FHSPAR F   TW VYASQP  PNNR RPPPGVDTRIHWQN
Sbjct: 1   MAMAS-PIPNSLFLNRT--FHSPARTF--RTWPVYASQP--PNNRQRPPPGVDTRIHWQN 60

Query: 61  EEEGWIGGPT--HSHPDQRNENNNILAPSLADLLNNSSDSHYQFLGVSAEADLEEIKSAY 120
           +EEGWIGGPT   S  DQ+ +NNN L PS ADLLNNSSDSHYQFLGV AEADLEEIKSAY
Sbjct: 61  DEEGWIGGPTPSQSRRDQKEQNNNALGPSFADLLNNSSDSHYQFLGVGAEADLEEIKSAY 120

Query: 121 RRLSKEYHPDTTSLPLKAASDKFMKLKQVYEVLSNEESRKFYDWTLAQEEASRQASKLRI 180
           RRLSKEYHPDTTSLPLKAASDKFMKLKQVYEVLSNEESRKFYDWTLAQEEASRQA KLRI
Sbjct: 121 RRLSKEYHPDTTSLPLKAASDKFMKLKQVYEVLSNEESRKFYDWTLAQEEASRQADKLRI 180

Query: 181 KLEDPYEQQLQNWKSTPDMVDRLAGRNMDLSDQATTALTLDILIILFAICCIVYVLLFKE 240
           KLEDPYEQ+LQNWKSTPDMVDRLAGRNMDLSDQATTALTLDILIILFAI CIVYVLLFKE
Sbjct: 181 KLEDPYEQELQNWKSTPDMVDRLAGRNMDLSDQATTALTLDILIILFAIGCIVYVLLFKE 236

Query: 241 PYY 242
           PYY
Sbjct: 241 PYY 236

[Close]

BLAST of Lag0030473 vs. ExPASy TrEMBL
Match: A0A6J1HJ56 (NAD(P)H-quinone oxidoreductase subunit T, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111464036 PE=4 SV=1)

HSP 1 Score: 394.4 bits (1012), Expect = 3.3e-106
Identity = 212/245 (86.53%), Postives = 217/245 (88.57%), Query Frame = 0


Query: 3   MASPPIPSSLFLRRSFRFHSPARIFNINTWRVYASQPPSPNNRPRPPPGVDTRIHWQNEE 62
           MASP IP+SLFL R+  FHSPAR F   TW VYASQP  PNNR RPPPGVDTRIHWQN+E
Sbjct: 1   MASPSIPNSLFLNRT--FHSPARTF--RTWPVYASQP--PNNRQRPPPGVDTRIHWQNDE 60

Query: 63  EGWIGGPT--HSHPDQR----NENNNILAPSLADLLNNSSDSHYQFLGVSAEADLEEIKS 122
           EGWIGGPT   S  DQ+    N NNN L PSL DLLNNSSDSHYQFLGV AEADLEEIKS
Sbjct: 61  EGWIGGPTPSQSRRDQKEQNNNNNNNALGPSLTDLLNNSSDSHYQFLGVGAEADLEEIKS 120

Query: 123 AYRRLSKEYHPDTTSLPLKAASDKFMKLKQVYEVLSNEESRKFYDWTLAQEEASRQASKL 182
           AYRRLSKEYHPDTTSLPLKAASDKFMKLKQVYEVLSNEESRKFYDWTLAQEEASRQA KL
Sbjct: 121 AYRRLSKEYHPDTTSLPLKAASDKFMKLKQVYEVLSNEESRKFYDWTLAQEEASRQADKL 180

Query: 183 RIKLEDPYEQQLQNWKSTPDMVDRLAGRNMDLSDQATTALTLDILIILFAICCIVYVLLF 242
           RIKLEDPYEQ+LQNWKSTPDMVDRLAGRNMDLSDQATTALTLDILIILFAI CIVYVLLF
Sbjct: 181 RIKLEDPYEQELQNWKSTPDMVDRLAGRNMDLSDQATTALTLDILIILFAIGCIVYVLLF 239

[Close]

BLAST of Lag0030473 vs. ExPASy TrEMBL
Match: A0A5D3BJW7 (NAD(P)H-quinone oxidoreductase subunit T OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G002250 PE=4 SV=1)

HSP 1 Score: 376.3 bits (965), Expect = 9.4e-101
Identity = 195/239 (81.59%), Postives = 210/239 (87.87%), Query Frame = 0


Query: 3   MASPPIPSSLFLRRSFRFHSPARIFNINTWRVYASQPPSPNNRPRPPPGVDTRIHWQNEE 62
           MAS P P+SL   ++  FHSPA  F   TWRVYA+Q P+ N+R RPPPGVDTRIHWQNEE
Sbjct: 1   MASSPFPNSLLFHKT--FHSPAPAF--RTWRVYAAQAPN-NDRRRPPPGVDTRIHWQNEE 60

Query: 63  EGWIGGPTHSHPDQRNENNNILAPSLADLLNNSSDSHYQFLGVSAEADLEEIKSAYRRLS 122
           EGWIGG      DQ NE+NN+L PSLADLLNNSSDSHYQFLGV AEA++EEIKSAYRRLS
Sbjct: 61  EGWIGGKKKESNDQNNESNNMLGPSLADLLNNSSDSHYQFLGVGAEAEVEEIKSAYRRLS 120

Query: 123 KEYHPDTTSLPLKAASDKFMKLKQVYEVLSNEESRKFYDWTLAQEEASRQASKLRIKLED 182
           KEYHPDTTSLPLK AS+KFMKLKQVYEVLSNEESRKFYDWTLAQEEASRQA KLRIKLED
Sbjct: 121 KEYHPDTTSLPLKVASEKFMKLKQVYEVLSNEESRKFYDWTLAQEEASRQADKLRIKLED 180

Query: 183 PYEQQLQNWKSTPDMVDRLAGRNMDLSDQATTALTLDILIILFAICCIVYVLLFKEPYY 242
           PYEQ+LQNW+STPDMVDRL GRNM+LSDQATTALTLDI IILFAI CIVYVL+FKEPYY
Sbjct: 181 PYEQELQNWQSTPDMVDRLGGRNMELSDQATTALTLDIFIILFAIACIVYVLIFKEPYY 234

[Close]

BLAST of Lag0030473 vs. ExPASy TrEMBL
Match: A0A1S3CHS6 (NAD(P)H-quinone oxidoreductase subunit T, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103501040 PE=4 SV=1)

HSP 1 Score: 376.3 bits (965), Expect = 9.4e-101
Identity = 195/239 (81.59%), Postives = 210/239 (87.87%), Query Frame = 0


Query: 3   MASPPIPSSLFLRRSFRFHSPARIFNINTWRVYASQPPSPNNRPRPPPGVDTRIHWQNEE 62
           MAS P P+SL   ++  FHSPA  F   TWRVYA+Q P+ N+R RPPPGVDTRIHWQNEE
Sbjct: 1   MASSPFPNSLLFHKT--FHSPAPAF--RTWRVYAAQAPN-NDRRRPPPGVDTRIHWQNEE 60

Query: 63  EGWIGGPTHSHPDQRNENNNILAPSLADLLNNSSDSHYQFLGVSAEADLEEIKSAYRRLS 122
           EGWIGG      DQ NE+NN+L PSLADLLNNSSDSHYQFLGV AEA++EEIKSAYRRLS
Sbjct: 61  EGWIGGKKKESNDQNNESNNMLGPSLADLLNNSSDSHYQFLGVGAEAEVEEIKSAYRRLS 120

Query: 123 KEYHPDTTSLPLKAASDKFMKLKQVYEVLSNEESRKFYDWTLAQEEASRQASKLRIKLED 182
           KEYHPDTTSLPLK AS+KFMKLKQVYEVLSNEESRKFYDWTLAQEEASRQA KLRIKLED
Sbjct: 121 KEYHPDTTSLPLKVASEKFMKLKQVYEVLSNEESRKFYDWTLAQEEASRQADKLRIKLED 180

Query: 183 PYEQQLQNWKSTPDMVDRLAGRNMDLSDQATTALTLDILIILFAICCIVYVLLFKEPYY 242
           PYEQ+LQNW+STPDMVDRL GRNM+LSDQATTALTLDI IILFAI CIVYVL+FKEPYY
Sbjct: 181 PYEQELQNWQSTPDMVDRLGGRNMELSDQATTALTLDIFIILFAIACIVYVLIFKEPYY 234

[Close]

BLAST of Lag0030473 vs. ExPASy TrEMBL
Match: A0A0A0LT25 (J domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G042670 PE=4 SV=1)

HSP 1 Score: 366.3 bits (939), Expect = 9.7e-98
Identity = 191/239 (79.92%), Postives = 207/239 (86.61%), Query Frame = 0


Query: 3   MASPPIPSSLFLRRSFRFHSPARIFNINTWRVYASQPPSPNNRPRPPPGVDTRIHWQNEE 62
           MASPP P+SL   ++  FHS A  F   TWRVYA+Q P+ N+R RPPPGVDTRIHWQNEE
Sbjct: 1   MASPPFPNSLLFHKT--FHSSAPTF--RTWRVYAAQAPN-NDRRRPPPGVDTRIHWQNEE 60

Query: 63  EGWIGGPTHSHPDQRNENNNILAPSLADLLNNSSDSHYQFLGVSAEADLEEIKSAYRRLS 122
           EGWIG       DQ N +NN+L PSLADLLNNSSDSHYQFLGV AEA++EEIKSAYRRLS
Sbjct: 61  EGWIGRKKKESNDQNNVSNNMLGPSLADLLNNSSDSHYQFLGVDAEAEVEEIKSAYRRLS 120

Query: 123 KEYHPDTTSLPLKAASDKFMKLKQVYEVLSNEESRKFYDWTLAQEEASRQASKLRIKLED 182
           KEYHPDTTSLPLK AS+KFMKLKQVYEVLSNEESRKFYDWTLAQEEASRQA KLR+KLED
Sbjct: 121 KEYHPDTTSLPLKVASEKFMKLKQVYEVLSNEESRKFYDWTLAQEEASRQADKLRMKLED 180

Query: 183 PYEQQLQNWKSTPDMVDRLAGRNMDLSDQATTALTLDILIILFAICCIVYVLLFKEPYY 242
           PYEQ+LQNW STPDMVDRL GRN++LSDQA+TALTLDI IILFAI CIVYVLLFKEPYY
Sbjct: 181 PYEQELQNWVSTPDMVDRLGGRNLELSDQASTALTLDIFIILFAIACIVYVLLFKEPYY 234

[Close]

BLAST of Lag0030473 vs. TAIR 10
Match: AT4G09350.1 (Chaperone DnaJ-domain superfamily protein )

HSP 1 Score: 272.7 bits (696), Expect = 2.8e-73
Identity = 138/210 (65.71%), Postives = 169/210 (80.48%), Query Frame = 0


Query: 33  RVYASQPPSPNNRPRPPPGVDTRIHWQNEEEGWIGGPTHSHPDQRNENNNILA-PSLADL 92
           R+YASQ   P    +P PGVDTRIHW++ +EGWIGG +        +  N+L+    A+L
Sbjct: 42  RIYASQ--GPTKPSKPSPGVDTRIHWESPDEGWIGGRSDPAKSVDEDKTNLLSDEKFAEL 101

Query: 93  LNNSSDSHYQFLGVSAEADLEEIKSAYRRLSKEYHPDTTSLPLKAASDKFMKLKQVYEVL 152
           + +S DSHYQFLGVS +ADLEEIKSAYRRLSKEYHPDTTSLPLK AS+KFMKL++VY VL
Sbjct: 102 IKDSFDSHYQFLGVSTDADLEEIKSAYRRLSKEYHPDTTSLPLKTASEKFMKLREVYNVL 161

Query: 153 SNEESRKFYDWTLAQEEASRQASKLRIKLEDPYEQQLQNWKSTPDMVDRLAGRNMDLSDQ 212
           S+EE+R+FYDWTLAQE ASRQA K+R+KLEDP EQ  + ++S PDMVDRL GRNM+LSDQ
Sbjct: 162 SDEETRRFYDWTLAQEVASRQAEKMRMKLEDPKEQDFRGYESIPDMVDRLGGRNMELSDQ 221

Query: 213 ATTALTLDILIILFAICCIVYVLLFKEPYY 242
           A TALT DILI+LFA+CCI +V++FK+P Y
Sbjct: 222 AMTALTFDILIVLFAVCCIAFVIVFKDPSY 249

[Close]

BLAST of Lag0030473 vs. TAIR 10
Match: AT3G17830.1 (Molecular chaperone Hsp40/DnaJ family protein )

HSP 1 Score: 65.5 bits (158), Expect = 6.8e-11
Identity = 32/63 (50.79%), Postives = 42/63 (66.67%), Query Frame = 0


Query: 99  HYQFLGVSAEADLEEIKSAYRRLSKEYHPDTTSLPLKAASDKFMKLKQVYEVLSNEESRK 158
           HY  L V+  A L+EIKS+YR+L+++YHPD    P   A DKF ++   YEVLS+EE R 
Sbjct: 64  HYSTLNVNRNATLQEIKSSYRKLARKYHPDMNKNP--GAEDKFKQISAAYEVLSDEEKRS 123

Query: 159 FYD 162
            YD
Sbjct: 124 AYD 124

[Close]

BLAST of Lag0030473 vs. TAIR 10
Match: AT1G80030.1 (Molecular chaperone Hsp40/DnaJ family protein )

HSP 1 Score: 64.7 bits (156), Expect = 1.2e-10
Identity = 32/67 (47.76%), Postives = 46/67 (68.66%), Query Frame = 0


Query: 95  SSDSHYQFLGVSAEADLEEIKSAYRRLSKEYHPDTTSLPLKAASDKFMKLKQVYEVLSNE 154
           +S  +Y  LGVS  A+ +EIK+AYRRL+++YHPD    P   A++KF ++   YEVLS+E
Sbjct: 72  TSGDYYATLGVSKSANNKEIKAAYRRLARQYHPDVNKEP--GATEKFKEISAAYEVLSDE 131

Query: 155 ESRKFYD 162
           + R  YD
Sbjct: 132 QKRALYD 136

[Close]

BLAST of Lag0030473 vs. TAIR 10
Match: AT1G80030.3 (Molecular chaperone Hsp40/DnaJ family protein )

HSP 1 Score: 64.7 bits (156), Expect = 1.2e-10
Identity = 32/67 (47.76%), Postives = 46/67 (68.66%), Query Frame = 0


Query: 95  SSDSHYQFLGVSAEADLEEIKSAYRRLSKEYHPDTTSLPLKAASDKFMKLKQVYEVLSNE 154
           +S  +Y  LGVS  A+ +EIK+AYRRL+++YHPD    P   A++KF ++   YEVLS+E
Sbjct: 72  TSGDYYATLGVSKSANNKEIKAAYRRLARQYHPDVNKEP--GATEKFKEISAAYEVLSDE 131

Query: 155 ESRKFYD 162
           + R  YD
Sbjct: 132 QKRALYD 136

[Close]

BLAST of Lag0030473 vs. TAIR 10
Match: AT1G80030.2 (Molecular chaperone Hsp40/DnaJ family protein )

HSP 1 Score: 64.7 bits (156), Expect = 1.2e-10
Identity = 32/67 (47.76%), Postives = 46/67 (68.66%), Query Frame = 0


Query: 95  SSDSHYQFLGVSAEADLEEIKSAYRRLSKEYHPDTTSLPLKAASDKFMKLKQVYEVLSNE 154
           +S  +Y  LGVS  A+ +EIK+AYRRL+++YHPD    P   A++KF ++   YEVLS+E
Sbjct: 72  TSGDYYATLGVSKSANNKEIKAAYRRLARQYHPDVNKEP--GATEKFKEISAAYEVLSDE 131

Query: 155 ESRKFYD 162
           + R  YD
Sbjct: 132 QKRALYD 136

The following BLAST results are available for this feature:

BLAST of Lag0030473 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022990181.1	6.2e-107	87.65	NAD(P)H-quinone oxidoreductase subunit T, chloroplastic [Cucurbita maxima]	[more]
KAG7033427.1	1.8e-106	86.23	NAD(P)H-quinone oxidoreductase subunit T, chloroplastic [Cucurbita argyrosperma ...	[more]
KAG6602740.1	1.8e-106	86.23	NAD(P)H-quinone oxidoreductase subunit T, chloroplastic, partial [Cucurbita argy...	[more]
XP_023521950.1	3.1e-106	86.23	NAD(P)H-quinone oxidoreductase subunit T, chloroplastic-like [Cucurbita pepo sub...	[more]
XP_022963850.1	6.9e-106	86.53	NAD(P)H-quinone oxidoreductase subunit T, chloroplastic [Cucurbita moschata]	[more]

BLAST of Lag0030473 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SMS0	4.0e-72	65.71	NAD(P)H-quinone oxidoreductase subunit T, chloroplastic OS=Arabidopsis thaliana ...	[more]
Q75VW3	5.1e-11	55.56	Chaperone protein DnaJ OS=Hydrogenobacter thermophilus (strain DSM 6534 / IAM 12...	[more]
Q5KWZ8	1.1e-10	52.38	Chaperone protein DnaJ OS=Geobacillus kaustophilus (strain HTA426) OX=235909 GN=...	[more]
Q45552	1.5e-10	52.38	Chaperone protein DnaJ OS=Geobacillus stearothermophilus OX=1422 GN=dnaJ PE=3 SV...	[more]
C5D4U0	1.9e-10	52.38	Chaperone protein DnaJ OS=Geobacillus sp. (strain WCH70) OX=471223 GN=dnaJ PE=3 ...	[more]

BLAST of Lag0030473 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1JM84	3.0e-107	87.65	NAD(P)H-quinone oxidoreductase subunit T, chloroplastic OS=Cucurbita maxima OX=3...	[more]
A0A6J1HJ56	3.3e-106	86.53	NAD(P)H-quinone oxidoreductase subunit T, chloroplastic OS=Cucurbita moschata OX...	[more]
A0A5D3BJW7	9.4e-101	81.59	NAD(P)H-quinone oxidoreductase subunit T OS=Cucumis melo var. makuwa OX=1194695 ...	[more]
A0A1S3CHS6	9.4e-101	81.59	NAD(P)H-quinone oxidoreductase subunit T, chloroplastic OS=Cucumis melo OX=3656 ...	[more]
A0A0A0LT25	9.7e-98	79.92	J domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G042670 PE=4 SV=1	[more]

BLAST of Lag0030473 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G09350.1	2.8e-73	65.71	Chaperone DnaJ-domain superfamily protein	[more]
AT3G17830.1	6.8e-11	50.79	Molecular chaperone Hsp40/DnaJ family protein	[more]
AT1G80030.1	1.2e-10	47.76	Molecular chaperone Hsp40/DnaJ family protein	[more]
AT1G80030.3	1.2e-10	47.76	Molecular chaperone Hsp40/DnaJ family protein	[more]
AT1G80030.2	1.2e-10	47.76	Molecular chaperone Hsp40/DnaJ family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 134..154
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 35..55
IPR001623	DnaJ domain	PRINTS	PR00625	JDOMAIN	coord: 100..118 score: 49.88 coord: 136..156 score: 35.13 coord: 118..133 score: 43.45
IPR001623	DnaJ domain	SMART	SM00271	dnaj_3	coord: 97..156 e-value: 4.2E-19 score: 79.5
IPR001623	DnaJ domain	PFAM	PF00226	DnaJ	coord: 99..161 e-value: 3.7E-20 score: 71.8
IPR001623	DnaJ domain	PROSITE	PS50076	DNAJ_2	coord: 98..164 score: 18.288189
IPR001623	DnaJ domain	CDD	cd06257	DnaJ	coord: 99..153 e-value: 4.72923E-18 score: 73.3485
IPR036869	Chaperone J-domain superfamily	GENE3D	1.10.287.110	DnaJ domain	coord: 85..186 e-value: 2.0E-21 score: 78.2
IPR036869	Chaperone J-domain superfamily	SUPERFAMILY	46565	Chaperone J-domain	coord: 92..164
IPR044618	NAD(P)H-quinone oxidoreductase subunit T-like	PANTHER	PTHR45283	NAD(P)H-QUINONE OXIDOREDUCTASE SUBUNIT T, CHLOROPLASTIC	coord: 4..240
IPR018253	DnaJ domain, conserved site	PROSITE	PS00636	DNAJ_1	coord: 141..160

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0030473.1	Lag0030473.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0009535	chloroplast thylakoid membrane
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0010598	NAD(P)H dehydrogenase complex (plastoquinone)