Lag0030460 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0030460
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
Descriptionglucuronoxylan 4-O-methyltransferase 3-like
Locationchr8: 47429674 .. 47430582 (+)
RNA-Seq ExpressionLag0030460
SyntenyLag0030460
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGCATGAGGTCCAGAGCCCCAGTCTCCATTAACCTCAAGCTCATTCTGTTTCTGTTTCTGTTTCTTTCCTTGCTCCTATTAGTTATCAGGTCAGGTTTTCAATCTTCCCAGAATAATAATAGTCCACCGCCCGTTGCACAAACTCATGTCTCCAATTTGGCAGATCCTGCGGAAGAACCATTGTCAATGCCAACAACAGCATGCCCATCTCTTCCCTTCACTCCAAGTTGCACAAAAACCCCGCCTTCTCTCGCCAACGCTCTCATCCACTACGCAACCACCAACATAACCCCTCAACAAACCCTCAAGGAGATCTCAGTGTCCGCCAGAATCCTCGAAACAAAGTCACCCTGCAACTTCTTGGTCTTTGGATTGGGCCATGACAGCCTCATGTGGACAGCTCTCAACCATGGAGGCCACACCGTTTTCCTCGAGGAAGACAAGGCCTGGATCGACCAAATACAGGACCGCCTGCCCTCCCTCGAGGCCTATCACGTGCAGTACGACACCAAGGTCCATCAAGCGGATGAGCTGATGAAGATGGGGCTGGAAGAAGAGTGCAAGGCCGTGGGAGACCCGAGATTTTCCAAGTGTCAGCTTGCGCACAAAGGCTTTCCGAGCGAAGTTTATGACATGGAATGGGACTTGATAATGGTGGATGCTCCTACTGGGTACTTCAACGAGGCGCCCGGAAGGATGAGTGCAATCTACACGGCTGGCCTCATGGCAAGGAACAGAGAGGAGGGTGACACTGATGTCTTCGTTCATGATGTAGACAGAGTGGTCGAGGACAATTTCTCTATGGCTTTTCTCTGTGAAGGATATCTCAGGAAACAAGAGGGCAGGATTAGGCACTTCACCATTCCAAGTCATAGGACTCGCCGGGGCAGACCTTTTTGCCCGTAG

mRNA sequence

ATGAGCATGAGGTCCAGAGCCCCAGTCTCCATTAACCTCAAGCTCATTCTGTTTCTGTTTCTGTTTCTTTCCTTGCTCCTATTAGTTATCAGGTCAGGTTTTCAATCTTCCCAGAATAATAATAGTCCACCGCCCGTTGCACAAACTCATGTCTCCAATTTGGCAGATCCTGCGGAAGAACCATTGTCAATGCCAACAACAGCATGCCCATCTCTTCCCTTCACTCCAAGTTGCACAAAAACCCCGCCTTCTCTCGCCAACGCTCTCATCCACTACGCAACCACCAACATAACCCCTCAACAAACCCTCAAGGAGATCTCAGTGTCCGCCAGAATCCTCGAAACAAAGTCACCCTGCAACTTCTTGGTCTTTGGATTGGGCCATGACAGCCTCATGTGGACAGCTCTCAACCATGGAGGCCACACCGTTTTCCTCGAGGAAGACAAGGCCTGGATCGACCAAATACAGGACCGCCTGCCCTCCCTCGAGGCCTATCACGTGCAGTACGACACCAAGGTCCATCAAGCGGATGAGCTGATGAAGATGGGGCTGGAAGAAGAGTGCAAGGCCGTGGGAGACCCGAGATTTTCCAAGTGTCAGCTTGCGCACAAAGGCTTTCCGAGCGAAGTTTATGACATGGAATGGGACTTGATAATGGTGGATGCTCCTACTGGGTACTTCAACGAGGCGCCCGGAAGGATGAGTGCAATCTACACGGCTGGCCTCATGGCAAGGAACAGAGAGGAGGGTGACACTGATGTCTTCGTTCATGATGTAGACAGAGTGGTCGAGGACAATTTCTCTATGGCTTTTCTCTGTGAAGGATATCTCAGGAAACAAGAGGGCAGGATTAGGCACTTCACCATTCCAAGTCATAGGACTCGCCGGGGCAGACCTTTTTGCCCGTAG

Coding sequence (CDS)

ATGAGCATGAGGTCCAGAGCCCCAGTCTCCATTAACCTCAAGCTCATTCTGTTTCTGTTTCTGTTTCTTTCCTTGCTCCTATTAGTTATCAGGTCAGGTTTTCAATCTTCCCAGAATAATAATAGTCCACCGCCCGTTGCACAAACTCATGTCTCCAATTTGGCAGATCCTGCGGAAGAACCATTGTCAATGCCAACAACAGCATGCCCATCTCTTCCCTTCACTCCAAGTTGCACAAAAACCCCGCCTTCTCTCGCCAACGCTCTCATCCACTACGCAACCACCAACATAACCCCTCAACAAACCCTCAAGGAGATCTCAGTGTCCGCCAGAATCCTCGAAACAAAGTCACCCTGCAACTTCTTGGTCTTTGGATTGGGCCATGACAGCCTCATGTGGACAGCTCTCAACCATGGAGGCCACACCGTTTTCCTCGAGGAAGACAAGGCCTGGATCGACCAAATACAGGACCGCCTGCCCTCCCTCGAGGCCTATCACGTGCAGTACGACACCAAGGTCCATCAAGCGGATGAGCTGATGAAGATGGGGCTGGAAGAAGAGTGCAAGGCCGTGGGAGACCCGAGATTTTCCAAGTGTCAGCTTGCGCACAAAGGCTTTCCGAGCGAAGTTTATGACATGGAATGGGACTTGATAATGGTGGATGCTCCTACTGGGTACTTCAACGAGGCGCCCGGAAGGATGAGTGCAATCTACACGGCTGGCCTCATGGCAAGGAACAGAGAGGAGGGTGACACTGATGTCTTCGTTCATGATGTAGACAGAGTGGTCGAGGACAATTTCTCTATGGCTTTTCTCTGTGAAGGATATCTCAGGAAACAAGAGGGCAGGATTAGGCACTTCACCATTCCAAGTCATAGGACTCGCCGGGGCAGACCTTTTTGCCCGTAG

Protein sequence

MSMRSRAPVSINLKLILFLFLFLSLLLLVIRSGFQSSQNNNSPPPVAQTHVSNLADPAEEPLSMPTTACPSLPFTPSCTKTPPSLANALIHYATTNITPQQTLKEISVSARILETKSPCNFLVFGLGHDSLMWTALNHGGHTVFLEEDKAWIDQIQDRLPSLEAYHVQYDTKVHQADELMKMGLEEECKAVGDPRFSKCQLAHKGFPSEVYDMEWDLIMVDAPTGYFNEAPGRMSAIYTAGLMARNREEGDTDVFVHDVDRVVEDNFSMAFLCEGYLRKQEGRIRHFTIPSHRTRRGRPFCP
Homology
BLAST of Lag0030460 vs. NCBI nr
Match: XP_023550528.1 (glucuronoxylan 4-O-methyltransferase 3-like [Cucurbita pepo subsp. pepo] >KAG6578305.1 Glucuronoxylan 4-O-methyltransferase 3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 528.1 bits (1359), Expect = 5.0e-146
Identity = 264/302 (87.42%), Postives = 276/302 (91.39%), Query Frame = 0

Query: 1   MSMRSRAPVSINLKLILFLFLFLSLLLLVIRSGFQSSQNNNSPPPVAQTHVSNLADPAEE 60
           MSMRSR P+SINLKLIL LF FLS+ +LVIRSG QSSQN   P PVAQTHVSNL    EE
Sbjct: 1   MSMRSRPPLSINLKLILLLFFFLSIFMLVIRSGLQSSQNR--PSPVAQTHVSNL---TEE 60

Query: 61  PLSMPTTACPSLPFTPSCTKTPPSLANALIHYATTNITPQQTLKEISVSARILETKSPCN 120
           P      ACPSLPFTPSCTKTPPSLANALIHYATTNITPQQTLKEISVSAR+LE KSPCN
Sbjct: 61  P------ACPSLPFTPSCTKTPPSLANALIHYATTNITPQQTLKEISVSARVLERKSPCN 120

Query: 121 FLVFGLGHDSLMWTALNHGGHTVFLEEDKAWIDQIQDRLPSLEAYHVQYDTKVHQADELM 180
           FLVFGLGHDSLMWTALNHGG TVFLEEDKAWIDQIQ+R+PSLEAY+VQYDTKVHQA+ELM
Sbjct: 121 FLVFGLGHDSLMWTALNHGGRTVFLEEDKAWIDQIQERVPSLEAYNVQYDTKVHQAEELM 180

Query: 181 KMGLEEECKAVGDPRFSKCQLAHKGFPSEVYDMEWDLIMVDAPTGYFNEAPGRMSAIYTA 240
           KMG+EEECK VGDPRFSKCQLA KGFP+E+YDMEWDLIMVDAPTGYFNEAPGRMSAIYTA
Sbjct: 181 KMGMEEECKTVGDPRFSKCQLALKGFPTEIYDMEWDLIMVDAPTGYFNEAPGRMSAIYTA 240

Query: 241 GLMARNREEGDTDVFVHDVDRVVEDNFSMAFLCEGYLRKQEGRIRHFTIPSHRTRRGRPF 300
           GLMARNR+EGDTDVFVHDVDRVVED FSMAFLCEGYLRKQEGRIRHFTIPSHRTR  RPF
Sbjct: 241 GLMARNRQEGDTDVFVHDVDRVVEDKFSMAFLCEGYLRKQEGRIRHFTIPSHRTRGSRPF 291

Query: 301 CP 303
           CP
Sbjct: 301 CP 291

BLAST of Lag0030460 vs. NCBI nr
Match: XP_022939329.1 (glucuronoxylan 4-O-methyltransferase 3-like [Cucurbita moschata] >KAG7015877.1 Glucuronoxylan 4-O-methyltransferase 3, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 526.9 bits (1356), Expect = 1.1e-145
Identity = 263/302 (87.09%), Postives = 276/302 (91.39%), Query Frame = 0

Query: 1   MSMRSRAPVSINLKLILFLFLFLSLLLLVIRSGFQSSQNNNSPPPVAQTHVSNLADPAEE 60
           MSMRSR P+SINLKLIL LF FLS+ +LVI+SG QSSQN   P PVAQTHVSNL    EE
Sbjct: 1   MSMRSRPPLSINLKLILLLFFFLSIFMLVIKSGLQSSQNR--PSPVAQTHVSNL---TEE 60

Query: 61  PLSMPTTACPSLPFTPSCTKTPPSLANALIHYATTNITPQQTLKEISVSARILETKSPCN 120
           P      ACPSLPFTPSCTKTPPSLANALIHYATTNITPQQTLKEISVSAR+LE KSPCN
Sbjct: 61  P------ACPSLPFTPSCTKTPPSLANALIHYATTNITPQQTLKEISVSARVLERKSPCN 120

Query: 121 FLVFGLGHDSLMWTALNHGGHTVFLEEDKAWIDQIQDRLPSLEAYHVQYDTKVHQADELM 180
           FLVFGLGHDSLMWTALNHGG TVFLEEDKAWIDQIQ+R+PSLEAY+VQYDTKVHQA+ELM
Sbjct: 121 FLVFGLGHDSLMWTALNHGGRTVFLEEDKAWIDQIQERVPSLEAYNVQYDTKVHQAEELM 180

Query: 181 KMGLEEECKAVGDPRFSKCQLAHKGFPSEVYDMEWDLIMVDAPTGYFNEAPGRMSAIYTA 240
           KMG+EEECK VGDPRFSKCQLA KGFP+E+YDMEWDLIMVDAPTGYFNEAPGRMSAIYTA
Sbjct: 181 KMGMEEECKTVGDPRFSKCQLALKGFPTEIYDMEWDLIMVDAPTGYFNEAPGRMSAIYTA 240

Query: 241 GLMARNREEGDTDVFVHDVDRVVEDNFSMAFLCEGYLRKQEGRIRHFTIPSHRTRRGRPF 300
           GLMARNR+EGDTDVFVHDVDRVVED FSMAFLCEGYLRKQEGRIRHFTIPSHRTR  RPF
Sbjct: 241 GLMARNRQEGDTDVFVHDVDRVVEDKFSMAFLCEGYLRKQEGRIRHFTIPSHRTRGSRPF 291

Query: 301 CP 303
           CP
Sbjct: 301 CP 291

BLAST of Lag0030460 vs. NCBI nr
Match: XP_022993078.1 (glucuronoxylan 4-O-methyltransferase 1-like [Cucurbita maxima])

HSP 1 Score: 525.4 bits (1352), Expect = 3.2e-145
Identity = 262/302 (86.75%), Postives = 276/302 (91.39%), Query Frame = 0

Query: 1   MSMRSRAPVSINLKLILFLFLFLSLLLLVIRSGFQSSQNNNSPPPVAQTHVSNLADPAEE 60
           MSMRSR P+SINLKLIL LF FLS+ +LVIRSG QSSQN   P PVAQTHVSNL    EE
Sbjct: 1   MSMRSRPPLSINLKLILLLFFFLSIFMLVIRSGLQSSQNR--PSPVAQTHVSNL---TEE 60

Query: 61  PLSMPTTACPSLPFTPSCTKTPPSLANALIHYATTNITPQQTLKEISVSARILETKSPCN 120
           P      ACPSLPFTPSCTKTPPSLANALIHYATTNITPQQTLKEISVSAR+LE KSPCN
Sbjct: 61  P------ACPSLPFTPSCTKTPPSLANALIHYATTNITPQQTLKEISVSARVLERKSPCN 120

Query: 121 FLVFGLGHDSLMWTALNHGGHTVFLEEDKAWIDQIQDRLPSLEAYHVQYDTKVHQADELM 180
           FLVFGLGHDSLMWTALNHGG TVFLEEDKAWIDQIQ+R+P+LEAY+VQYDTKVHQA+ELM
Sbjct: 121 FLVFGLGHDSLMWTALNHGGRTVFLEEDKAWIDQIQERVPTLEAYNVQYDTKVHQAEELM 180

Query: 181 KMGLEEECKAVGDPRFSKCQLAHKGFPSEVYDMEWDLIMVDAPTGYFNEAPGRMSAIYTA 240
           K+G+EEECK VGDPRFSKCQLA KGFP+E+YDMEWDLIMVDAPTGYFNEAPGRMSAIYTA
Sbjct: 181 KIGMEEECKTVGDPRFSKCQLALKGFPTEIYDMEWDLIMVDAPTGYFNEAPGRMSAIYTA 240

Query: 241 GLMARNREEGDTDVFVHDVDRVVEDNFSMAFLCEGYLRKQEGRIRHFTIPSHRTRRGRPF 300
           GLMARNR+EGDTDVFVHDVDRVVED FSMAFLCEGYLRKQEGRIRHFTIPSHRTR  RPF
Sbjct: 241 GLMARNRQEGDTDVFVHDVDRVVEDKFSMAFLCEGYLRKQEGRIRHFTIPSHRTRGSRPF 291

Query: 301 CP 303
           CP
Sbjct: 301 CP 291

BLAST of Lag0030460 vs. NCBI nr
Match: XP_022152515.1 (glucuronoxylan 4-O-methyltransferase 3-like [Momordica charantia])

HSP 1 Score: 516.5 bits (1329), Expect = 1.5e-142
Identity = 262/304 (86.18%), Postives = 274/304 (90.13%), Query Frame = 0

Query: 1   MSMRSRAPVSINLKLILFLFLFLSLLLLVIRSGFQSSQNNNSPPPVAQTHVSNLADPAEE 60
           MS RSRAP+SINLKLIL LFLFLS+LLLVIRSGFQSSQ    P PVAQTHVSNL + AE 
Sbjct: 1   MSSRSRAPLSINLKLILLLFLFLSILLLVIRSGFQSSQ----PSPVAQTHVSNLTEEAE- 60

Query: 61  PLSMPTTACPS--LPFTPSCTKTPPSLANALIHYATTNITPQQTLKEISVSARILETKSP 120
                 +ACPS  LPFTPSCTKTPPSLANALIHYATTNITPQQTL EISVSA IL+ KSP
Sbjct: 61  ------SACPSLTLPFTPSCTKTPPSLANALIHYATTNITPQQTLNEISVSATILQRKSP 120

Query: 121 CNFLVFGLGHDSLMWTALNHGGHTVFLEEDKAWIDQIQDRLPSLEAYHVQYDTKVHQADE 180
           CNFLVFGLGHDSLMWTALNHGG TVFLEED+AWI QIQ RLPSLEAYHV YDTKVH+ADE
Sbjct: 121 CNFLVFGLGHDSLMWTALNHGGRTVFLEEDEAWIHQIQQRLPSLEAYHVHYDTKVHEADE 180

Query: 181 LMKMGLEEECKAVGDPRFSKCQLAHKGFPSEVYDMEWDLIMVDAPTGYFNEAPGRMSAIY 240
           LMKMG+EEEC+AVGDPRFSKCQLA K FPSEVYDMEWDLIMVDAPTGYFNEAPGRMSAIY
Sbjct: 181 LMKMGMEEECRAVGDPRFSKCQLALKRFPSEVYDMEWDLIMVDAPTGYFNEAPGRMSAIY 240

Query: 241 TAGLMARNREEGDTDVFVHDVDRVVEDNFSMAFLCEGYLRKQEGRIRHFTIPSHRTRRGR 300
           TAGLMARNR EGDTDVFVHDVDRVVED FSMAFLCEGYLR+Q+GRIRHF IPSHRTR+GR
Sbjct: 241 TAGLMARNRVEGDTDVFVHDVDRVVEDKFSMAFLCEGYLREQQGRIRHFNIPSHRTRQGR 293

Query: 301 PFCP 303
           PFCP
Sbjct: 301 PFCP 293

BLAST of Lag0030460 vs. NCBI nr
Match: XP_038884332.1 (glucuronoxylan 4-O-methyltransferase 3-like [Benincasa hispida])

HSP 1 Score: 503.1 bits (1294), Expect = 1.7e-138
Identity = 255/303 (84.16%), Postives = 270/303 (89.11%), Query Frame = 0

Query: 1   MSMR-SRAPVSINLKLILFLFLFLSLLLLVIRSGFQSSQNNNSPPPVAQTHVSNLADPAE 60
           MSMR SR P+SINLKLIL LFLFLS LLLVIRSGFQSSQ  NSPPP  QTH+SNL    E
Sbjct: 1   MSMRSSRPPLSINLKLILLLFLFLSFLLLVIRSGFQSSQ--NSPPPATQTHLSNL---TE 60

Query: 61  EPLSMPTTACPSLPFTPSCTKTPPSLANALIHYATTNITPQQTLKEISVSARILETKSPC 120
           EP       CPS P T  CTKTP S+ANALIHYATTN+TPQQTLKEISVSA ILE KSPC
Sbjct: 61  EP------ECPSPPTTSICTKTPASVANALIHYATTNVTPQQTLKEISVSATILEAKSPC 120

Query: 121 NFLVFGLGHDSLMWTALNHGGHTVFLEEDKAWIDQIQDRLPSLEAYHVQYDTKVHQADEL 180
           NFLVFGLGHDSLMWTALNHGG TVFLEEDKAWI+QIQ+RLP+LEAYHVQYDTKVHQADEL
Sbjct: 121 NFLVFGLGHDSLMWTALNHGGRTVFLEEDKAWIEQIQERLPNLEAYHVQYDTKVHQADEL 180

Query: 181 MKMGLEEECKAVGDPRFSKCQLAHKGFPSEVYDMEWDLIMVDAPTGYFNEAPGRMSAIYT 240
           MKMG+EEECK+VGDPRFS+CQLA KGFPSE+Y+MEWDLIMVDAPTGYFNEAPGRMSAIYT
Sbjct: 181 MKMGMEEECKSVGDPRFSECQLALKGFPSEIYEMEWDLIMVDAPTGYFNEAPGRMSAIYT 240

Query: 241 AGLMARNREEGDTDVFVHDVDRVVEDNFSMAFLCEGYLRKQEGRIRHFTIPSHRTRRGRP 300
           AGLMARNR EG+TDVFVHDVDRVVED+FSMAFLC+GYLRKQEGRIRHFTIPSHR R   P
Sbjct: 241 AGLMARNRREGETDVFVHDVDRVVEDSFSMAFLCQGYLRKQEGRIRHFTIPSHRPRPDTP 292

Query: 301 FCP 303
           FCP
Sbjct: 301 FCP 292

BLAST of Lag0030460 vs. ExPASy Swiss-Prot
Match: Q9LQ32 (Glucuronoxylan 4-O-methyltransferase 3 OS=Arabidopsis thaliana OX=3702 GN=GXM3 PE=1 SV=1)

HSP 1 Score: 370.9 bits (951), Expect = 1.3e-101
Identity = 179/300 (59.67%), Postives = 233/300 (77.67%), Query Frame = 0

Query: 3   MRSRAPVSINLKLILFLFLFLSLLLLVIRSGFQSSQNNNSPPPVAQTHVSNLADPAEEPL 62
           MR+++P S+NLK+I   F+  S+L+L+I    +S+ +++S  P+++T++S      EE  
Sbjct: 1   MRTKSPSSLNLKVI---FIGSSILILIIIYLARSNISSSSSKPISKTNLSQ----EEEET 60

Query: 63  SMPTTACPSLPFTPSCTKTPPSLANALIHYATTNITPQQTLKEISVSARILETKSPCNFL 122
                 CP+   T  CTK P SL++AL+HY T+N+TPQQT  E+SVS R+L+ KSPCNFL
Sbjct: 61  QHKQEGCPT---TQQCTKMPLSLSDALVHYVTSNVTPQQTFDEVSVSKRVLDKKSPCNFL 120

Query: 123 VFGLGHDSLMWTALNHGGHTVFLEEDKAWIDQIQDRLPSLEAYHVQYDTKVHQADELMKM 182
           VFGLGHDSLMW +LNHGG T+F+EED+AWI  +  + P+LE+YHV YDTKV  +D+LM++
Sbjct: 121 VFGLGHDSLMWASLNHGGRTLFIEEDQAWIAIVTKKFPNLESYHVVYDTKVKDSDKLMEL 180

Query: 183 GLEEECKAVGDPRFSKCQLAHKGFPSEVYDMEWDLIMVDAPTGYFNEAPGRMSAIYTAGL 242
           G  EEC++V DPR SKC LA K FP++ Y+ +WDLIMVDAPTGY  EAPGRMSAIYTAGL
Sbjct: 181 GRSEECRSVSDPRNSKCDLALKDFPADFYETKWDLIMVDAPTGYHEEAPGRMSAIYTAGL 240

Query: 243 MARNREEGDTDVFVHDVDRVVEDNFSMAFLCEGYLRKQEGRIRHFTIPSHRTRRGRPFCP 302
           +ARNRE+G+TDVFVHDV+R VED FS  FLC+GY+R+Q GR+RHFTIPSHR R GRPFCP
Sbjct: 241 LARNREDGETDVFVHDVNRPVEDEFSATFLCKGYMREQNGRLRHFTIPSHRARAGRPFCP 290

BLAST of Lag0030460 vs. ExPASy Swiss-Prot
Match: Q6NMK1 (Glucuronoxylan 4-O-methyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=GXM1 PE=1 SV=1)

HSP 1 Score: 351.7 bits (901), Expect = 8.4e-96
Identity = 176/305 (57.70%), Postives = 222/305 (72.79%), Query Frame = 0

Query: 3   MRSRAPVSINLKLILFLFLFLSLLLLVIRSGFQSSQNNNSPPPVAQTHVSNLADPAEEPL 62
           MR +A  +  LK++L   L   +L+ ++RS   SSQ + +P     T  S          
Sbjct: 1   MRPKANQNHKLKVLLVFLLATLILIFIVRSTLTSSQEHQTPQETRSTRCSG--------- 60

Query: 63  SMPTTACPSLPFTPSCTKTPPSLANALIHYATTNITPQQTLKEISVSARILETKSPCNFL 122
                         +C K P SLA ALIHY+T+ ITPQQTLKEI+VS+R+L  KSPCNFL
Sbjct: 61  --------------ACNKLPRSLAQALIHYSTSVITPQQTLKEIAVSSRVLGKKSPCNFL 120

Query: 123 VFGLGHDSLMWTALNHGGHTVFLEEDKAWIDQIQDRLPSLEAYHVQYDTKVHQADELMKM 182
           VFGLGHDSLMW++LN+GG TVFLEED+AWI QI+ R P LE+YHV YD+KV+QAD L+++
Sbjct: 121 VFGLGHDSLMWSSLNYGGRTVFLEEDEAWIKQIKRRFPMLESYHVTYDSKVNQADNLIEV 180

Query: 183 GLEEECKAVGDPRFSKCQLAHKGFPSEVYDMEWDLIMVDAPTGYFNEAPGRMSAIYTAGL 242
           G   EC A+GDPR+S CQLA KG P+E+Y+  WDLIMVDAPTGY++EAPGRM+AIYTAG+
Sbjct: 181 GKGPECTAIGDPRYSMCQLALKGLPAEIYETGWDLIMVDAPTGYYDEAPGRMTAIYTAGM 240

Query: 243 MARNREE-GDTDVFVHDVDRVVEDNFSMAFLCEGYLRKQEGRIRHFTIPSHR----TRRG 302
           MARNR++ G+TDVFVHDV+R +ED FS AFLCEGY++KQEGR+RHF IPS+R    +   
Sbjct: 241 MARNRKQGGETDVFVHDVNREIEDKFSKAFLCEGYMKKQEGRLRHFIIPSYRDGSESESN 282

BLAST of Lag0030460 vs. ExPASy Swiss-Prot
Match: Q9T0F7 (Glucuronoxylan 4-O-methyltransferase 2 OS=Arabidopsis thaliana OX=3702 GN=GXM2 PE=1 SV=1)

HSP 1 Score: 339.3 bits (869), Expect = 4.3e-92
Identity = 169/301 (56.15%), Postives = 222/301 (73.75%), Query Frame = 0

Query: 3   MRSRAPVSINLKLILFLFLFLSLLLLVIRSGFQSSQNNNSPPPVAQTHVSNLADPAEEPL 62
           MR+++   I+ KLI   F+  S+L+L I    ++S ++NS   +   +            
Sbjct: 1   MRNKSQSFISSKLI---FICCSILVLFILFLKRASFSSNSTATIRDEYHQK--------- 60

Query: 63  SMPTTACPSLPFTPSCTKTPPSLANALIHYATTNITPQQTLKEISVSARILETKSPCNFL 122
               + CPS P    CTK P SL++AL+HY T+ ITPQQT  E+SVS R+L+ KSPCNFL
Sbjct: 61  ----SKCPSTP--QQCTKLPTSLSDALVHYVTSEITPQQTFDEVSVSKRVLDKKSPCNFL 120

Query: 123 VFGLGHDSLMWTALNHGGHTVFLEEDKAWIDQIQDRLPSLEAYHVQYDTKVHQADELMKM 182
           VFGLGHDSLMW +LNHGG T+FLEED+AWI+ +  + P+LE+YHV YDTKV  +++LM++
Sbjct: 121 VFGLGHDSLMWASLNHGGRTLFLEEDEAWIETVTKKFPNLESYHVVYDTKVKDSNKLMEL 180

Query: 183 GLEEECKAVGDPRFSKCQLAHKGFPSEVYDMEWDLIMVDAPTGYFNEAPGRMSAIYTAGL 242
              E+CKAV DPR SKC L+ KGFP++VY+ +WD+IMVDAPTGY +EAPGRMSAIYTAGL
Sbjct: 181 KRTEDCKAVSDPRDSKCALSLKGFPADVYETQWDVIMVDAPTGYHDEAPGRMSAIYTAGL 240

Query: 243 MARNR-EEGDTDVFVHDVDRVVEDNFSMAFLCEGYLRKQEGRIRHFTIPSHRTRRGRPFC 302
           +ARNR + G+TDVFVHD++R VED FS+AFLC GY+++Q+GR+RHF IPSHR   G PFC
Sbjct: 241 LARNRYDGGETDVFVHDINRPVEDEFSVAFLCGGYMKEQQGRLRHFNIPSHRASFGTPFC 283

BLAST of Lag0030460 vs. ExPASy Swiss-Prot
Match: Q9C7F9 (Probable methyltransferase At1g27930 OS=Arabidopsis thaliana OX=3702 GN=At1g27930 PE=1 SV=1)

HSP 1 Score: 217.2 bits (552), Expect = 2.5e-55
Identity = 110/213 (51.64%), Postives = 141/213 (66.20%), Query Frame = 0

Query: 83  PSLANALIHYATTNITPQQTLKEISVSARILETKSPCNFLVFGLGHDSLMWTALNHGGHT 142
           P    A++HYAT+N+ PQQ L EIS+S  IL+  +P NFLVFGLG DSLMW +LN  G T
Sbjct: 62  PIQLQAIVHYATSNVVPQQNLAEISISFNILKKLAPANFLVFGLGRDSLMWASLNPRGKT 121

Query: 143 VFLEEDKAWIDQIQDRLPSLEAYHVQYDTKVHQADELMKMGLEEECKAVGDPRF----SK 202
           +FLEED  W  ++    P L A+HV+Y T++ QAD L++   + E K      +     K
Sbjct: 122 LFLEEDLEWFQKVTKDSPFLRAHHVRYRTQLQQADSLLR-SYKTEPKCFPAKSYLRGNEK 181

Query: 203 CQLAHKGFPSEVYDMEWDLIMVDAPTGYFNEAPGRMSAIYTAGLMARNREE-GDTDVFVH 262
           C+LA  G P E YD EWDL+MVDAP GYF EAPGRM+AI++A +MARNR++ G T VF+H
Sbjct: 182 CKLALTGLPDEFYDTEWDLLMVDAPKGYFAEAPGRMAAIFSAAVMARNRKKPGVTHVFLH 241

Query: 263 DVDRVVEDNFSMAFLCEGYLRKQEGRIRHFTIP 291
           DV+R VE  F+  FLC  Y     GR+ HF IP
Sbjct: 242 DVNRRVEKTFAEEFLCRKYRVNAAGRLWHFAIP 273

BLAST of Lag0030460 vs. ExPASy Swiss-Prot
Match: Q9SNE5 (Protein IRREGULAR XYLEM 15 OS=Arabidopsis thaliana OX=3702 GN=IRX15 PE=2 SV=1)

HSP 1 Score: 163.7 bits (413), Expect = 3.3e-39
Identity = 90/227 (39.65%), Postives = 133/227 (58.59%), Query Frame = 0

Query: 70  PSLPFTPSCTKTPPSLANALIHYAT-TNITPQQTLKEI-SVSARILETKSPCNFLVFGLG 129
           PS     S +  P S  NAL+HYA+ +N +   +  E+ S+S  +     PCN LVFGL 
Sbjct: 73  PSASSPISNSNLPTSAINALLHYASRSNDSFHMSYGEMKSISDVLRRCAPPCNLLVFGLT 132

Query: 130 HDSLMWTALNHGGHTVFLEEDKAWIDQIQDRLPSLEAYHVQYDTKVHQADELMKMGLE-- 189
           H++L+W +LNH G TVF+EE++ +    ++  P ++ + VQY TK H+A EL+    E  
Sbjct: 133 HETLLWKSLNHNGRTVFIEENRYYAAYFEEIHPEIDVFDVQYTTKAHEAGELVTAAKEAA 192

Query: 190 -EECKAVGDPRFSKCQLAHKGFPSEVYDMEWDLIMVDAPTGYFNEAPGRMSAIYTAGLMA 249
             EC+ V +  FS C+L     P+ VYD++WD+I VD P G  +E PGRMS+I+TA ++A
Sbjct: 193 GNECRPVQNLLFSDCKLGLNDLPNHVYDVDWDVIFVDGPRGDAHEGPGRMSSIFTAAVLA 252

Query: 250 RNREEG--DTDVFVHDVDRVVEDNFSMAFLCEGYLRKQEGRIRHFTI 290
           R+++ G   T VFVHD  R VE      FLC   L +    + H+ +
Sbjct: 253 RSKKGGTPKTHVFVHDYYRDVERLCGDEFLCRENLVESNDLLAHYVL 299

BLAST of Lag0030460 vs. ExPASy TrEMBL
Match: A0A6J1FGV0 (glucuronoxylan 4-O-methyltransferase 3-like OS=Cucurbita moschata OX=3662 GN=LOC111445277 PE=4 SV=1)

HSP 1 Score: 526.9 bits (1356), Expect = 5.4e-146
Identity = 263/302 (87.09%), Postives = 276/302 (91.39%), Query Frame = 0

Query: 1   MSMRSRAPVSINLKLILFLFLFLSLLLLVIRSGFQSSQNNNSPPPVAQTHVSNLADPAEE 60
           MSMRSR P+SINLKLIL LF FLS+ +LVI+SG QSSQN   P PVAQTHVSNL    EE
Sbjct: 1   MSMRSRPPLSINLKLILLLFFFLSIFMLVIKSGLQSSQNR--PSPVAQTHVSNL---TEE 60

Query: 61  PLSMPTTACPSLPFTPSCTKTPPSLANALIHYATTNITPQQTLKEISVSARILETKSPCN 120
           P      ACPSLPFTPSCTKTPPSLANALIHYATTNITPQQTLKEISVSAR+LE KSPCN
Sbjct: 61  P------ACPSLPFTPSCTKTPPSLANALIHYATTNITPQQTLKEISVSARVLERKSPCN 120

Query: 121 FLVFGLGHDSLMWTALNHGGHTVFLEEDKAWIDQIQDRLPSLEAYHVQYDTKVHQADELM 180
           FLVFGLGHDSLMWTALNHGG TVFLEEDKAWIDQIQ+R+PSLEAY+VQYDTKVHQA+ELM
Sbjct: 121 FLVFGLGHDSLMWTALNHGGRTVFLEEDKAWIDQIQERVPSLEAYNVQYDTKVHQAEELM 180

Query: 181 KMGLEEECKAVGDPRFSKCQLAHKGFPSEVYDMEWDLIMVDAPTGYFNEAPGRMSAIYTA 240
           KMG+EEECK VGDPRFSKCQLA KGFP+E+YDMEWDLIMVDAPTGYFNEAPGRMSAIYTA
Sbjct: 181 KMGMEEECKTVGDPRFSKCQLALKGFPTEIYDMEWDLIMVDAPTGYFNEAPGRMSAIYTA 240

Query: 241 GLMARNREEGDTDVFVHDVDRVVEDNFSMAFLCEGYLRKQEGRIRHFTIPSHRTRRGRPF 300
           GLMARNR+EGDTDVFVHDVDRVVED FSMAFLCEGYLRKQEGRIRHFTIPSHRTR  RPF
Sbjct: 241 GLMARNRQEGDTDVFVHDVDRVVEDKFSMAFLCEGYLRKQEGRIRHFTIPSHRTRGSRPF 291

Query: 301 CP 303
           CP
Sbjct: 301 CP 291

BLAST of Lag0030460 vs. ExPASy TrEMBL
Match: A0A6J1K144 (glucuronoxylan 4-O-methyltransferase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111489201 PE=4 SV=1)

HSP 1 Score: 525.4 bits (1352), Expect = 1.6e-145
Identity = 262/302 (86.75%), Postives = 276/302 (91.39%), Query Frame = 0

Query: 1   MSMRSRAPVSINLKLILFLFLFLSLLLLVIRSGFQSSQNNNSPPPVAQTHVSNLADPAEE 60
           MSMRSR P+SINLKLIL LF FLS+ +LVIRSG QSSQN   P PVAQTHVSNL    EE
Sbjct: 1   MSMRSRPPLSINLKLILLLFFFLSIFMLVIRSGLQSSQNR--PSPVAQTHVSNL---TEE 60

Query: 61  PLSMPTTACPSLPFTPSCTKTPPSLANALIHYATTNITPQQTLKEISVSARILETKSPCN 120
           P      ACPSLPFTPSCTKTPPSLANALIHYATTNITPQQTLKEISVSAR+LE KSPCN
Sbjct: 61  P------ACPSLPFTPSCTKTPPSLANALIHYATTNITPQQTLKEISVSARVLERKSPCN 120

Query: 121 FLVFGLGHDSLMWTALNHGGHTVFLEEDKAWIDQIQDRLPSLEAYHVQYDTKVHQADELM 180
           FLVFGLGHDSLMWTALNHGG TVFLEEDKAWIDQIQ+R+P+LEAY+VQYDTKVHQA+ELM
Sbjct: 121 FLVFGLGHDSLMWTALNHGGRTVFLEEDKAWIDQIQERVPTLEAYNVQYDTKVHQAEELM 180

Query: 181 KMGLEEECKAVGDPRFSKCQLAHKGFPSEVYDMEWDLIMVDAPTGYFNEAPGRMSAIYTA 240
           K+G+EEECK VGDPRFSKCQLA KGFP+E+YDMEWDLIMVDAPTGYFNEAPGRMSAIYTA
Sbjct: 181 KIGMEEECKTVGDPRFSKCQLALKGFPTEIYDMEWDLIMVDAPTGYFNEAPGRMSAIYTA 240

Query: 241 GLMARNREEGDTDVFVHDVDRVVEDNFSMAFLCEGYLRKQEGRIRHFTIPSHRTRRGRPF 300
           GLMARNR+EGDTDVFVHDVDRVVED FSMAFLCEGYLRKQEGRIRHFTIPSHRTR  RPF
Sbjct: 241 GLMARNRQEGDTDVFVHDVDRVVEDKFSMAFLCEGYLRKQEGRIRHFTIPSHRTRGSRPF 291

Query: 301 CP 303
           CP
Sbjct: 301 CP 291

BLAST of Lag0030460 vs. ExPASy TrEMBL
Match: A0A6J1DE55 (glucuronoxylan 4-O-methyltransferase 3-like OS=Momordica charantia OX=3673 GN=LOC111020222 PE=4 SV=1)

HSP 1 Score: 516.5 bits (1329), Expect = 7.3e-143
Identity = 262/304 (86.18%), Postives = 274/304 (90.13%), Query Frame = 0

Query: 1   MSMRSRAPVSINLKLILFLFLFLSLLLLVIRSGFQSSQNNNSPPPVAQTHVSNLADPAEE 60
           MS RSRAP+SINLKLIL LFLFLS+LLLVIRSGFQSSQ    P PVAQTHVSNL + AE 
Sbjct: 1   MSSRSRAPLSINLKLILLLFLFLSILLLVIRSGFQSSQ----PSPVAQTHVSNLTEEAE- 60

Query: 61  PLSMPTTACPS--LPFTPSCTKTPPSLANALIHYATTNITPQQTLKEISVSARILETKSP 120
                 +ACPS  LPFTPSCTKTPPSLANALIHYATTNITPQQTL EISVSA IL+ KSP
Sbjct: 61  ------SACPSLTLPFTPSCTKTPPSLANALIHYATTNITPQQTLNEISVSATILQRKSP 120

Query: 121 CNFLVFGLGHDSLMWTALNHGGHTVFLEEDKAWIDQIQDRLPSLEAYHVQYDTKVHQADE 180
           CNFLVFGLGHDSLMWTALNHGG TVFLEED+AWI QIQ RLPSLEAYHV YDTKVH+ADE
Sbjct: 121 CNFLVFGLGHDSLMWTALNHGGRTVFLEEDEAWIHQIQQRLPSLEAYHVHYDTKVHEADE 180

Query: 181 LMKMGLEEECKAVGDPRFSKCQLAHKGFPSEVYDMEWDLIMVDAPTGYFNEAPGRMSAIY 240
           LMKMG+EEEC+AVGDPRFSKCQLA K FPSEVYDMEWDLIMVDAPTGYFNEAPGRMSAIY
Sbjct: 181 LMKMGMEEECRAVGDPRFSKCQLALKRFPSEVYDMEWDLIMVDAPTGYFNEAPGRMSAIY 240

Query: 241 TAGLMARNREEGDTDVFVHDVDRVVEDNFSMAFLCEGYLRKQEGRIRHFTIPSHRTRRGR 300
           TAGLMARNR EGDTDVFVHDVDRVVED FSMAFLCEGYLR+Q+GRIRHF IPSHRTR+GR
Sbjct: 241 TAGLMARNRVEGDTDVFVHDVDRVVEDKFSMAFLCEGYLREQQGRIRHFNIPSHRTRQGR 293

Query: 301 PFCP 303
           PFCP
Sbjct: 301 PFCP 293

BLAST of Lag0030460 vs. ExPASy TrEMBL
Match: A0A0A0LMN7 (Polysacc_synt_4 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G396200 PE=4 SV=1)

HSP 1 Score: 501.1 bits (1289), Expect = 3.2e-138
Identity = 256/303 (84.49%), Postives = 269/303 (88.78%), Query Frame = 0

Query: 1   MSMRS-RAPVSINLKLILFLFLFLSLLLLVIRSGFQSSQNNNSPPPVAQTHVSNLADPAE 60
           MSMRS R P+SINLKLIL LFLFLS LLLVIRSGFQSSQNN  PPPV QTHV  L D  E
Sbjct: 1   MSMRSTRPPLSINLKLILLLFLFLSFLLLVIRSGFQSSQNN--PPPVTQTHV--LTDEPE 60

Query: 61  EPLSMPTTACPSLPFTPSCTKTPPSLANALIHYATTNITPQQTLKEISVSARILETKSPC 120
                    CPSLP    CTKTPPS+ANALIHYATTNITPQQTLKEISVSARILETKSPC
Sbjct: 61  ---------CPSLPI---CTKTPPSVANALIHYATTNITPQQTLKEISVSARILETKSPC 120

Query: 121 NFLVFGLGHDSLMWTALNHGGHTVFLEEDKAWIDQIQDRLPSLEAYHVQYDTKVHQADEL 180
           NFLVFGLGHDSLMWTALNHGG TVFLEEDKAWIDQIQ+RLP+LEAYHVQYDTKVHQADEL
Sbjct: 121 NFLVFGLGHDSLMWTALNHGGRTVFLEEDKAWIDQIQERLPNLEAYHVQYDTKVHQADEL 180

Query: 181 MKMGLEEECKAVGDPRFSKCQLAHKGFPSEVYDMEWDLIMVDAPTGYFNEAPGRMSAIYT 240
           MK+G+ EECK VGDPRF KC+LA KGFPSE+Y+MEWDLIMVDAPTGYFNEAPGRMSAIYT
Sbjct: 181 MKIGMGEECKIVGDPRFCKCELALKGFPSEIYEMEWDLIMVDAPTGYFNEAPGRMSAIYT 240

Query: 241 AGLMARNREEGDTDVFVHDVDRVVEDNFSMAFLCEGYLRKQEGRIRHFTIPSHRTRRGRP 300
           AGLMARNR+EG+TDVFVHDVDRVVEDNFSMAFLC+GYL KQEGRIRHF+IPSHRTR   P
Sbjct: 241 AGLMARNRQEGETDVFVHDVDRVVEDNFSMAFLCKGYLTKQEGRIRHFSIPSHRTRPDTP 287

Query: 301 FCP 303
           FCP
Sbjct: 301 FCP 287

BLAST of Lag0030460 vs. ExPASy TrEMBL
Match: A0A1S3B2B1 (glucuronoxylan 4-O-methyltransferase 3-like OS=Cucumis melo OX=3656 GN=LOC103485376 PE=4 SV=1)

HSP 1 Score: 498.8 bits (1283), Expect = 1.6e-137
Identity = 253/303 (83.50%), Postives = 270/303 (89.11%), Query Frame = 0

Query: 1   MSMR-SRAPVSINLKLILFLFLFLSLLLLVIRSGFQSSQNNNSPPPVAQTHVSNLADPAE 60
           MSMR +R P+SINLKLIL LFLFLS LLLVIRSGFQSSQNN  PPPV QTH+  L D  E
Sbjct: 1   MSMRATRPPLSINLKLILLLFLFLSFLLLVIRSGFQSSQNN--PPPVTQTHI--LTDEPE 60

Query: 61  EPLSMPTTACPSLPFTPSCTKTPPSLANALIHYATTNITPQQTLKEISVSARILETKSPC 120
                    CPSLP    CTKTPPS+ANALIHYAT+NITPQQTLKEISVSARILETKSPC
Sbjct: 61  ---------CPSLPI---CTKTPPSVANALIHYATSNITPQQTLKEISVSARILETKSPC 120

Query: 121 NFLVFGLGHDSLMWTALNHGGHTVFLEEDKAWIDQIQDRLPSLEAYHVQYDTKVHQADEL 180
           NFLVFGLGHDSLMWTALNHGG TVFLEEDKAWIDQIQ+RLP+LEAYHVQYDTKVHQADEL
Sbjct: 121 NFLVFGLGHDSLMWTALNHGGRTVFLEEDKAWIDQIQERLPNLEAYHVQYDTKVHQADEL 180

Query: 181 MKMGLEEECKAVGDPRFSKCQLAHKGFPSEVYDMEWDLIMVDAPTGYFNEAPGRMSAIYT 240
           MK+G+ EECK+VGDPRF KC+LA KG PSE+Y+MEWDLIMVDAPTGYFNEAPGRMSAIYT
Sbjct: 181 MKIGMGEECKSVGDPRFCKCELALKGLPSEIYEMEWDLIMVDAPTGYFNEAPGRMSAIYT 240

Query: 241 AGLMARNREEGDTDVFVHDVDRVVEDNFSMAFLCEGYLRKQEGRIRHFTIPSHRTRRGRP 300
           AGLMARNR+EG+TDVFVHDVDRVVEDNFSMAFLC+GYLRKQEGRIRHF+IPSHRTR   P
Sbjct: 241 AGLMARNRQEGETDVFVHDVDRVVEDNFSMAFLCKGYLRKQEGRIRHFSIPSHRTRPDTP 287

Query: 301 FCP 303
           FCP
Sbjct: 301 FCP 287

BLAST of Lag0030460 vs. TAIR 10
Match: AT1G33800.1 (Protein of unknown function (DUF579) )

HSP 1 Score: 370.9 bits (951), Expect = 9.5e-103
Identity = 179/300 (59.67%), Postives = 233/300 (77.67%), Query Frame = 0

Query: 3   MRSRAPVSINLKLILFLFLFLSLLLLVIRSGFQSSQNNNSPPPVAQTHVSNLADPAEEPL 62
           MR+++P S+NLK+I   F+  S+L+L+I    +S+ +++S  P+++T++S      EE  
Sbjct: 1   MRTKSPSSLNLKVI---FIGSSILILIIIYLARSNISSSSSKPISKTNLSQ----EEEET 60

Query: 63  SMPTTACPSLPFTPSCTKTPPSLANALIHYATTNITPQQTLKEISVSARILETKSPCNFL 122
                 CP+   T  CTK P SL++AL+HY T+N+TPQQT  E+SVS R+L+ KSPCNFL
Sbjct: 61  QHKQEGCPT---TQQCTKMPLSLSDALVHYVTSNVTPQQTFDEVSVSKRVLDKKSPCNFL 120

Query: 123 VFGLGHDSLMWTALNHGGHTVFLEEDKAWIDQIQDRLPSLEAYHVQYDTKVHQADELMKM 182
           VFGLGHDSLMW +LNHGG T+F+EED+AWI  +  + P+LE+YHV YDTKV  +D+LM++
Sbjct: 121 VFGLGHDSLMWASLNHGGRTLFIEEDQAWIAIVTKKFPNLESYHVVYDTKVKDSDKLMEL 180

Query: 183 GLEEECKAVGDPRFSKCQLAHKGFPSEVYDMEWDLIMVDAPTGYFNEAPGRMSAIYTAGL 242
           G  EEC++V DPR SKC LA K FP++ Y+ +WDLIMVDAPTGY  EAPGRMSAIYTAGL
Sbjct: 181 GRSEECRSVSDPRNSKCDLALKDFPADFYETKWDLIMVDAPTGYHEEAPGRMSAIYTAGL 240

Query: 243 MARNREEGDTDVFVHDVDRVVEDNFSMAFLCEGYLRKQEGRIRHFTIPSHRTRRGRPFCP 302
           +ARNRE+G+TDVFVHDV+R VED FS  FLC+GY+R+Q GR+RHFTIPSHR R GRPFCP
Sbjct: 241 LARNREDGETDVFVHDVNRPVEDEFSATFLCKGYMREQNGRLRHFTIPSHRARAGRPFCP 290

BLAST of Lag0030460 vs. TAIR 10
Match: AT1G09610.1 (Protein of unknown function (DUF579) )

HSP 1 Score: 351.7 bits (901), Expect = 6.0e-97
Identity = 176/305 (57.70%), Postives = 222/305 (72.79%), Query Frame = 0

Query: 3   MRSRAPVSINLKLILFLFLFLSLLLLVIRSGFQSSQNNNSPPPVAQTHVSNLADPAEEPL 62
           MR +A  +  LK++L   L   +L+ ++RS   SSQ + +P     T  S          
Sbjct: 1   MRPKANQNHKLKVLLVFLLATLILIFIVRSTLTSSQEHQTPQETRSTRCSG--------- 60

Query: 63  SMPTTACPSLPFTPSCTKTPPSLANALIHYATTNITPQQTLKEISVSARILETKSPCNFL 122
                         +C K P SLA ALIHY+T+ ITPQQTLKEI+VS+R+L  KSPCNFL
Sbjct: 61  --------------ACNKLPRSLAQALIHYSTSVITPQQTLKEIAVSSRVLGKKSPCNFL 120

Query: 123 VFGLGHDSLMWTALNHGGHTVFLEEDKAWIDQIQDRLPSLEAYHVQYDTKVHQADELMKM 182
           VFGLGHDSLMW++LN+GG TVFLEED+AWI QI+ R P LE+YHV YD+KV+QAD L+++
Sbjct: 121 VFGLGHDSLMWSSLNYGGRTVFLEEDEAWIKQIKRRFPMLESYHVTYDSKVNQADNLIEV 180

Query: 183 GLEEECKAVGDPRFSKCQLAHKGFPSEVYDMEWDLIMVDAPTGYFNEAPGRMSAIYTAGL 242
           G   EC A+GDPR+S CQLA KG P+E+Y+  WDLIMVDAPTGY++EAPGRM+AIYTAG+
Sbjct: 181 GKGPECTAIGDPRYSMCQLALKGLPAEIYETGWDLIMVDAPTGYYDEAPGRMTAIYTAGM 240

Query: 243 MARNREE-GDTDVFVHDVDRVVEDNFSMAFLCEGYLRKQEGRIRHFTIPSHR----TRRG 302
           MARNR++ G+TDVFVHDV+R +ED FS AFLCEGY++KQEGR+RHF IPS+R    +   
Sbjct: 241 MARNRKQGGETDVFVHDVNREIEDKFSKAFLCEGYMKKQEGRLRHFIIPSYRDGSESESN 282

BLAST of Lag0030460 vs. TAIR 10
Match: AT4G09990.1 (Protein of unknown function (DUF579) )

HSP 1 Score: 339.3 bits (869), Expect = 3.1e-93
Identity = 169/301 (56.15%), Postives = 222/301 (73.75%), Query Frame = 0

Query: 3   MRSRAPVSINLKLILFLFLFLSLLLLVIRSGFQSSQNNNSPPPVAQTHVSNLADPAEEPL 62
           MR+++   I+ KLI   F+  S+L+L I    ++S ++NS   +   +            
Sbjct: 1   MRNKSQSFISSKLI---FICCSILVLFILFLKRASFSSNSTATIRDEYHQK--------- 60

Query: 63  SMPTTACPSLPFTPSCTKTPPSLANALIHYATTNITPQQTLKEISVSARILETKSPCNFL 122
               + CPS P    CTK P SL++AL+HY T+ ITPQQT  E+SVS R+L+ KSPCNFL
Sbjct: 61  ----SKCPSTP--QQCTKLPTSLSDALVHYVTSEITPQQTFDEVSVSKRVLDKKSPCNFL 120

Query: 123 VFGLGHDSLMWTALNHGGHTVFLEEDKAWIDQIQDRLPSLEAYHVQYDTKVHQADELMKM 182
           VFGLGHDSLMW +LNHGG T+FLEED+AWI+ +  + P+LE+YHV YDTKV  +++LM++
Sbjct: 121 VFGLGHDSLMWASLNHGGRTLFLEEDEAWIETVTKKFPNLESYHVVYDTKVKDSNKLMEL 180

Query: 183 GLEEECKAVGDPRFSKCQLAHKGFPSEVYDMEWDLIMVDAPTGYFNEAPGRMSAIYTAGL 242
              E+CKAV DPR SKC L+ KGFP++VY+ +WD+IMVDAPTGY +EAPGRMSAIYTAGL
Sbjct: 181 KRTEDCKAVSDPRDSKCALSLKGFPADVYETQWDVIMVDAPTGYHDEAPGRMSAIYTAGL 240

Query: 243 MARNR-EEGDTDVFVHDVDRVVEDNFSMAFLCEGYLRKQEGRIRHFTIPSHRTRRGRPFC 302
           +ARNR + G+TDVFVHD++R VED FS+AFLC GY+++Q+GR+RHF IPSHR   G PFC
Sbjct: 241 LARNRYDGGETDVFVHDINRPVEDEFSVAFLCGGYMKEQQGRLRHFNIPSHRASFGTPFC 283

BLAST of Lag0030460 vs. TAIR 10
Match: AT1G71690.1 (Protein of unknown function (DUF579) )

HSP 1 Score: 311.6 bits (797), Expect = 6.9e-85
Identity = 169/302 (55.96%), Postives = 206/302 (68.21%), Query Frame = 0

Query: 8   PVSINLKLILFLFLFLSLLLLVIRSGFQSSQNNNSPPPVAQTHVSNLADPAEEPLSMPTT 67
           P++  L L  F+ L +  L  + R+GF  S              S+   P    L + T+
Sbjct: 8   PMTTRLILSSFVSLVVLTLFFITRTGFSPS--------------SSFHQPLNNTLRISTS 67

Query: 68  ACPSLPFTP-SCTKTPPSLANALIHYATTNITPQQTLKEISVSARILETKSPCNFLVFGL 127
           +  S   +P SC K PPSLA+AL+HYA++N+TPQQTL EISV+ + LE KSPCNFLVFGL
Sbjct: 68  STGSKLQSPRSCNKIPPSLADALVHYASSNVTPQQTLSEISVTKKELEKKSPCNFLVFGL 127

Query: 128 GHDSLMWTALNHGGHTVFLEEDKAWIDQIQDRLPSLEAYHVQYDTKVHQADELMKMGLE- 187
           GHDSLMW  LNHGG T+FL+ED++WI QI ++ PSLE+YHV+Y TKV  A+ LM    + 
Sbjct: 128 GHDSLMWATLNHGGRTIFLDEDESWIHQIAEKFPSLESYHVRYKTKVRDAEALMAATKDR 187

Query: 188 EECKAVG-DPRFSKCQLAHKGFPSEVYDMEWDLIMVDAPTGYFNEAPGRMSAIYTAGLMA 247
           EEC+ V  D R S C+LA KG P  VY+ EWDLIMVDAPTG+  EAPGRMSAIYTAG++A
Sbjct: 188 EECRRVSTDLRVSTCELALKGLPEVVYETEWDLIMVDAPTGFHEEAPGRMSAIYTAGMIA 247

Query: 248 RNR--EEGDTDVFVHDVDRVVEDNFSMAFLCEGYLRKQEGRIRHFTIPSHRTR--RGRPF 303
           R R  EE  T VFVHDVDR VED FSM FLC  Y+ KQEGR+RHFT+PSHR     G  F
Sbjct: 248 RRRKDEEETTAVFVHDVDRKVEDEFSMEFLCRDYMTKQEGRLRHFTVPSHRNYGVSGVKF 295

BLAST of Lag0030460 vs. TAIR 10
Match: AT1G67330.1 (Protein of unknown function (DUF579) )

HSP 1 Score: 221.9 bits (564), Expect = 7.1e-58
Identity = 121/259 (46.72%), Postives = 160/259 (61.78%), Query Frame = 0

Query: 51  VSNLADPAEEPLSMPTTACPSLPFTPSCTKTPPSLANALIHYATTNITPQQTLKEISVSA 110
           +++     +  LS+ +TA  +       T TP  L  +++HYAT++  PQQ+ +EIS+S 
Sbjct: 34  ITSFIRATDNTLSLCSTAKNTAASIAKYTATPIQL-QSIVHYATSHTVPQQSFEEISISL 93

Query: 111 RILETKSPCNFLVFGLGHDSLMWTALNHGGHTVFLEEDKAWIDQIQDRLPSLEAYHVQYD 170
            +L+ + PCNFLVFGLG DSLMW +LN GG TVFLEED  WI+ +    PSL A+HVQY 
Sbjct: 94  NVLKERLPCNFLVFGLGRDSLMWASLNPGGTTVFLEEDPEWIEAVLKDAPSLRAHHVQYR 153

Query: 171 TKVHQADELMKMGLEE----ECKAVGDPRFSKCQLAHKGFPSEVYDMEWDLIMVDAPTGY 230
           T + +A  L+     E      KA       KC LA    P E YD EWDLIMVDAP GY
Sbjct: 154 THLSEAGRLLSTYKNEPMCLPAKAFPIRYNEKCPLALTSLPDEFYDTEWDLIMVDAPKGY 213

Query: 231 FNEAPGRMSAIYTAGLMARNRE-EGDTDVFVHDVDRVVEDNFSMAFLCEGYLRKQEGRIR 290
           F EAPGRM+AI+++ +MARNR+ +G T VF+HDV+R VE+ F+  FLCE Y     GR+ 
Sbjct: 214 FPEAPGRMAAIFSSAIMARNRKGDGTTHVFLHDVNRKVENAFANEFLCEKYKVNSVGRLW 273

Query: 291 HFTIPSHRTRRGRP---FC 302
           HF IP+      +P   FC
Sbjct: 274 HFEIPNAANMTDQPGDRFC 291

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_023550528.15.0e-14687.42glucuronoxylan 4-O-methyltransferase 3-like [Cucurbita pepo subsp. pepo] >KAG657... [more]
XP_022939329.11.1e-14587.09glucuronoxylan 4-O-methyltransferase 3-like [Cucurbita moschata] >KAG7015877.1 G... [more]
XP_022993078.13.2e-14586.75glucuronoxylan 4-O-methyltransferase 1-like [Cucurbita maxima][more]
XP_022152515.11.5e-14286.18glucuronoxylan 4-O-methyltransferase 3-like [Momordica charantia][more]
XP_038884332.11.7e-13884.16glucuronoxylan 4-O-methyltransferase 3-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
Q9LQ321.3e-10159.67Glucuronoxylan 4-O-methyltransferase 3 OS=Arabidopsis thaliana OX=3702 GN=GXM3 P... [more]
Q6NMK18.4e-9657.70Glucuronoxylan 4-O-methyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=GXM1 P... [more]
Q9T0F74.3e-9256.15Glucuronoxylan 4-O-methyltransferase 2 OS=Arabidopsis thaliana OX=3702 GN=GXM2 P... [more]
Q9C7F92.5e-5551.64Probable methyltransferase At1g27930 OS=Arabidopsis thaliana OX=3702 GN=At1g2793... [more]
Q9SNE53.3e-3939.65Protein IRREGULAR XYLEM 15 OS=Arabidopsis thaliana OX=3702 GN=IRX15 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1FGV05.4e-14687.09glucuronoxylan 4-O-methyltransferase 3-like OS=Cucurbita moschata OX=3662 GN=LOC... [more]
A0A6J1K1441.6e-14586.75glucuronoxylan 4-O-methyltransferase 1-like OS=Cucurbita maxima OX=3661 GN=LOC11... [more]
A0A6J1DE557.3e-14386.18glucuronoxylan 4-O-methyltransferase 3-like OS=Momordica charantia OX=3673 GN=LO... [more]
A0A0A0LMN73.2e-13884.49Polysacc_synt_4 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G39... [more]
A0A1S3B2B11.6e-13783.50glucuronoxylan 4-O-methyltransferase 3-like OS=Cucumis melo OX=3656 GN=LOC103485... [more]
Match NameE-valueIdentityDescription
AT1G33800.19.5e-10359.67Protein of unknown function (DUF579) [more]
AT1G09610.16.0e-9757.70Protein of unknown function (DUF579) [more]
AT4G09990.13.1e-9356.15Protein of unknown function (DUF579) [more]
AT1G71690.16.9e-8555.96Protein of unknown function (DUF579) [more]
AT1G67330.17.1e-5846.72Protein of unknown function (DUF579) [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006514IRX15/IRX15L/IGXMTIGRFAMTIGR01627TIGR01627coord: 79..301
e-value: 2.4E-91
score: 303.4
IPR006514IRX15/IRX15L/IGXMPANTHERPTHR31444OS11G0490100 PROTEINcoord: 3..302
IPR021148Polysaccharide biosynthesis domainPFAMPF04669Polysacc_synt_4coord: 104..287
e-value: 3.1E-71
score: 238.9
NoneNo IPR availablePANTHERPTHR31444:SF26GLUCURONOXYLAN 4-O-METHYLTRANSFERASE 2-RELATEDcoord: 3..302

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0030460.1Lag0030460.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0032259 methylation
biological_process GO:0009834 plant-type secondary cell wall biogenesis
biological_process GO:0045492 xylan biosynthetic process
cellular_component GO:0000139 Golgi membrane
molecular_function GO:0030775 glucuronoxylan 4-O-methyltransferase activity