Lag0030408 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0030408
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	alkylated DNA repair protein alkB homolog 8
Location	chr8: 47022576 .. 47024757 (+)
RNA-Seq Expression	Lag0030408
Synteny	Lag0030408

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCCCCAAGCTTTTAGTTGTGCTTCGAGAGTGAGCAAACTCTTCTATTATAACAGCAAGATCTTTGGTTTATCCTCTGTTGTAAGTGGTAGCGGCCATCATTTTAGACTTTCCAGTGCTATGAAAGAGATTAAAGTCAAAGGTGGTTCTAATGCAGATCCTCATCTGTTGAATGATGAAACACATGCACAGCAATTGTCTTGTGCAAATCAGGGGTGTTCATCATCAAATGTGCAGTGCACCCCTGAAATTGAAAAGAAGTATGTGCATCGTGTTTATGATGCCATTGCTCCTCATTTTAGTTCCACTCGATTTGCAAAGTGGCCAAAAGTTGCATCATTCTTAACGTCATTACCTCTAGGGTCCCTCATCCTGGATGCTGGCTGTGGTAATGGAAAGTACTTGGGTTTTATTCCCAACTGCTTTTTTATTGGATGTGATATTAGTGCCCAACTTATCAAAATTTGTAATGAGAGAGGGCATGAAGTTTTGGTGGCTGATGCAGTAAATCTTCCTTACAGAACTGGTTTTGGCGATGCAGCAATCTCAATAGCCGTCTTACATCATTTAAGTACTGAGAACAGGAGGAAGAAAGCAATCGAAGAATTAATACGTGTTGTCAAAAAAGGTGGCCTAGTTTTAATAACAGTCTGGGCTGTTGAACAAGAGGATAAATCTTTATTGACGAAATGGATGCCGCTTTCAGAGAAGTATGTTGATGAGTGGGTTGGACCAGGTAGTCCGCGTGTTCGTAGTCCTACGTCATTGGCACTTGAAAGCATTCCTGAAATGAATGAAAACAATTCAGGTATTTATCTAAAGGATTCCAAGGAAAACTTCACTGGAAGCATGCCAGAAAATAAACCTCTACCCTCTCAAAGTGGAAATGATTTGGCAAATTGTAACGATGAAAATTTATTGAAGACCCAGCAAGAATATTTTGTCCCATGGCACTTACCTTATCATCGTGCAGAAGTCAGTGGCACGTCTGCCAGTGCCCTTGCTAGTGGCTTGGCAAAGAAAGATGATAAGAAAGCTGCTGTTGTGTATAACAGATACTATCATGTTTTCAGTGAAGGCGAGCTTGAAAGGTATTCCTCTCTCTCATCTCTCGTAAGGTTTGTTTGTTTGTTTGTGTGTGTTTTTTCCTCCCTTTCCCTCTCGTCTCCTTTTGCTAATTGGGTTATGAGTGAGTCACTTGAATACATCTTCTTTCAGATGTGACTGCTGTTCTAGATCTAGATGTGTTCAAGTGTGTCTTTAATTCCTTCCCTCTGATACTCAGACATAAGCCATATATTTTTGGTCAAAAAGTAGCTGGATGAAAGTTGGACAAAAGGGGACTGATGTAGACATTTTCTTATGCGTGCGAAAAATCTTCGTAATGTACTAACAAAGATGAAAGAAGCAAACAGTAGCTGTAAAATTTGTTTTTGCACATGGGAGATGATGCTGGTCCCGTCAATTAATCCTTTGTAGTCCATTTTAGAAATGTATGGGAAAGAACATTGTACTTTTACCAGCTTTGACTGTACAGAATTATTGTTTCCCTTCATGCAAATCGAACAGAAAATCCTACCTAGAAAAGAGATTTACAAAAAGGGAGGTGAGATAAGAAGACATCCATGCGTAGGAGGTAGGACAAAAAGGAGATCATAAAAAAGCTCTCCTCCAATTGGTAGCAATAAAAGAAAAGGAGAATACAATTTTGAGGCGAAAAGGGGAAAAAAGAAAAGGGATGGGACAGGAGAGCTTGGAAAAGACCCGAAAAGTAAGCCTGATCTTAGGCTGAAAGTTTTTCCTACCAGCTCTTCTTGGGGGCACTATTGGAAATTTCCATCTATACTTTGCTCCTGAAGTACATTTAATATCTGTTAGCACTAGCAGTACCTTGTTAACTCCTTCCAGTTCCATTCATGTGATGAGGAGATAATTGATAACCAGGATCATTCCCCAGTTTTCTATCATTCCATCTTTTAACTTACGAAATAGGGAACTCAGTGTATCGTATTTCAAGGCTTAAACTTCGTTTACCCCCTTGAGATGACTATAATTTGTTCTTTTACTGCTTGCTCATCTTGGGTGCTTTTGCAGGTTGGTTTCTGGTATGGACAATGCAGTTGTGGTCGATCGATTTTATGACAAATCGAACTGGTGTATAGTTCTCGAGAAAACGGTGTGA

mRNA sequence

ATGCCCCAAGCTTTTAGTTGTGCTTCGAGAGTGAGCAAACTCTTCTATTATAACAGCAAGATCTTTGGTTTATCCTCTGTTGTAAGTGGTAGCGGCCATCATTTTAGACTTTCCAGTGCTATGAAAGAGATTAAAGTCAAAGGTGGTTCTAATGCAGATCCTCATCTGTTGAATGATGAAACACATGCACAGCAATTGTCTTGTGCAAATCAGGGGTGTTCATCATCAAATGTGCAGTGCACCCCTGAAATTGAAAAGAAGTATGTGCATCGTGTTTATGATGCCATTGCTCCTCATTTTAGTTCCACTCGATTTGCAAAGTGGCCAAAAGTTGCATCATTCTTAACGTCATTACCTCTAGGGTCCCTCATCCTGGATGCTGGCTGTGGTAATGGAAAGTACTTGGGTTTTATTCCCAACTGCTTTTTTATTGGATGTGATATTAGTGCCCAACTTATCAAAATTTGTAATGAGAGAGGGCATGAAGTTTTGGTGGCTGATGCAGTAAATCTTCCTTACAGAACTGGTTTTGGCGATGCAGCAATCTCAATAGCCGTCTTACATCATTTAAGTACTGAGAACAGGAGGAAGAAAGCAATCGAAGAATTAATACGTGTTGTCAAAAAAGGTGGCCTAGTTTTAATAACAGTCTGGGCTGTTGAACAAGAGGATAAATCTTTATTGACGAAATGGATGCCGCTTTCAGAGAAGTATGTTGATGAGTGGGTTGGACCAGGTAGTCCGCGTGTTCGTAGTCCTACGTCATTGGCACTTGAAAGCATTCCTGAAATGAATGAAAACAATTCAGGTATTTATCTAAAGGATTCCAAGGAAAACTTCACTGGAAGCATGCCAGAAAATAAACCTCTACCCTCTCAAAGTGGAAATGATTTGGCAAATTGTAACGATGAAAATTTATTGAAGACCCAGCAAGAATATTTTGTCCCATGGCACTTACCTTATCATCGTGCAGAAGTCAGTGGCACGTCTGCCAGTGCCCTTGCTAGTGGCTTGGCAAAGAAAGATGATAAGAAAGCTGCTGTTGTGTATAACAGATACTATCATGTTTTCAGTGAAGGCGAGCTTGAAAGGTTGGTTTCTGGTATGGACAATGCAGTTGTGGTCGATCGATTTTATGACAAATCGAACTGGTGTATAGTTCTCGAGAAAACGGTGTGA

Coding sequence (CDS)

ATGCCCCAAGCTTTTAGTTGTGCTTCGAGAGTGAGCAAACTCTTCTATTATAACAGCAAGATCTTTGGTTTATCCTCTGTTGTAAGTGGTAGCGGCCATCATTTTAGACTTTCCAGTGCTATGAAAGAGATTAAAGTCAAAGGTGGTTCTAATGCAGATCCTCATCTGTTGAATGATGAAACACATGCACAGCAATTGTCTTGTGCAAATCAGGGGTGTTCATCATCAAATGTGCAGTGCACCCCTGAAATTGAAAAGAAGTATGTGCATCGTGTTTATGATGCCATTGCTCCTCATTTTAGTTCCACTCGATTTGCAAAGTGGCCAAAAGTTGCATCATTCTTAACGTCATTACCTCTAGGGTCCCTCATCCTGGATGCTGGCTGTGGTAATGGAAAGTACTTGGGTTTTATTCCCAACTGCTTTTTTATTGGATGTGATATTAGTGCCCAACTTATCAAAATTTGTAATGAGAGAGGGCATGAAGTTTTGGTGGCTGATGCAGTAAATCTTCCTTACAGAACTGGTTTTGGCGATGCAGCAATCTCAATAGCCGTCTTACATCATTTAAGTACTGAGAACAGGAGGAAGAAAGCAATCGAAGAATTAATACGTGTTGTCAAAAAAGGTGGCCTAGTTTTAATAACAGTCTGGGCTGTTGAACAAGAGGATAAATCTTTATTGACGAAATGGATGCCGCTTTCAGAGAAGTATGTTGATGAGTGGGTTGGACCAGGTAGTCCGCGTGTTCGTAGTCCTACGTCATTGGCACTTGAAAGCATTCCTGAAATGAATGAAAACAATTCAGGTATTTATCTAAAGGATTCCAAGGAAAACTTCACTGGAAGCATGCCAGAAAATAAACCTCTACCCTCTCAAAGTGGAAATGATTTGGCAAATTGTAACGATGAAAATTTATTGAAGACCCAGCAAGAATATTTTGTCCCATGGCACTTACCTTATCATCGTGCAGAAGTCAGTGGCACGTCTGCCAGTGCCCTTGCTAGTGGCTTGGCAAAGAAAGATGATAAGAAAGCTGCTGTTGTGTATAACAGATACTATCATGTTTTCAGTGAAGGCGAGCTTGAAAGGTTGGTTTCTGGTATGGACAATGCAGTTGTGGTCGATCGATTTTATGACAAATCGAACTGGTGTATAGTTCTCGAGAAAACGGTGTGA

Protein sequence

MPQAFSCASRVSKLFYYNSKIFGLSSVVSGSGHHFRLSSAMKEIKVKGGSNADPHLLNDETHAQQLSCANQGCSSSNVQCTPEIEKKYVHRVYDAIAPHFSSTRFAKWPKVASFLTSLPLGSLILDAGCGNGKYLGFIPNCFFIGCDISAQLIKICNERGHEVLVADAVNLPYRTGFGDAAISIAVLHHLSTENRRKKAIEELIRVVKKGGLVLITVWAVEQEDKSLLTKWMPLSEKYVDEWVGPGSPRVRSPTSLALESIPEMNENNSGIYLKDSKENFTGSMPENKPLPSQSGNDLANCNDENLLKTQQEYFVPWHLPYHRAEVSGTSASALASGLAKKDDKKAAVVYNRYYHVFSEGELERLVSGMDNAVVVDRFYDKSNWCIVLEKTV

Homology

[Close]

BLAST of Lag0030408 vs. NCBI nr
Match: XP_022939521.1 (alkylated DNA repair protein alkB homolog 8-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 738.0 bits (1904), Expect = 4.1e-209
Identity = 366/392 (93.37%), Postives = 372/392 (94.90%), Query Frame = 0


Query: 1   MPQAFSCASRVSKLFYYNSKIFGLSSVVSGSGHHFRLSSAMKEIKVKGGSNADPHLLNDE 60
           MPQAF  ASRVSK F   SKIFGLSSVVSGSGHH RL SAMKEIKVKGGSNADPHLLNDE
Sbjct: 54  MPQAFYWASRVSKRFCRTSKIFGLSSVVSGSGHHCRLFSAMKEIKVKGGSNADPHLLNDE 113

Query: 61  THAQQLSCANQGCSSSNVQCTPEIEKKYVHRVYDAIAPHFSSTRFAKWPKVASFLTSLPL 120
            HA QLSCANQGCS+SNVQCTPEIEKKYVHRVYDAIAPHFSSTRFAKWPKVASFL+SLP 
Sbjct: 114 AHAPQLSCANQGCSASNVQCTPEIEKKYVHRVYDAIAPHFSSTRFAKWPKVASFLSSLPR 173

Query: 121 GSLILDAGCGNGKYLGFIPNCFFIGCDISAQLIKICNERGHEVLVADAVNLPYRTGFGDA 180
           GSLILDAGCGNGKYLGF  NCFFIGCDIS QLIKICNERGHEVLVADAVNLPYRTGFGDA
Sbjct: 174 GSLILDAGCGNGKYLGFNLNCFFIGCDISPQLIKICNERGHEVLVADAVNLPYRTGFGDA 233

Query: 181 AISIAVLHHLSTENRRKKAIEELIRVVKKGGLVLITVWAVEQEDKSLLTKWMPLSEKYVD 240
           AISIAVLHHLSTENRRKKAIEEL+RVVKKGGLVLITVWAVEQEDKSLLTKWMPLSEKYV+
Sbjct: 234 AISIAVLHHLSTENRRKKAIEELLRVVKKGGLVLITVWAVEQEDKSLLTKWMPLSEKYVE 293

Query: 241 EWVGPGSPRVRSPTSLALESIPEMNENNSGIYLKDSKENFTGSMPENKPLPSQSGNDLAN 300
           EWVGPGSPRVRSP+SLALESIPEMNENNSG YLKDSK NFTGSMP N PLPSQSGNDLAN
Sbjct: 294 EWVGPGSPRVRSPSSLALESIPEMNENNSGTYLKDSKVNFTGSMPGNIPLPSQSGNDLAN 353

Query: 301 CNDENLLKTQQEYFVPWHLPYHRAEVSGTSASALASGLAKKDDKKAAVVYNRYYHVFSEG 360
           CNDENL KTQQEYFVPWHLPYHRAEVSGTSA ALASGLAKKDDKKAAVVYNRYYHVFSEG
Sbjct: 354 CNDENLWKTQQEYFVPWHLPYHRAEVSGTSAGALASGLAKKDDKKAAVVYNRYYHVFSEG 413

Query: 361 ELERLVSGMDNAVVVDRFYDKSNWCIVLEKTV 393
           ELERLVSGMDNAVVVDRFYDKSNWCIVLEKT+
Sbjct: 414 ELERLVSGMDNAVVVDRFYDKSNWCIVLEKTM 445

[Close]

BLAST of Lag0030408 vs. NCBI nr
Match: XP_022939523.1 (alkylated DNA repair protein alkB homolog 8-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 738.0 bits (1904), Expect = 4.1e-209
Identity = 366/392 (93.37%), Postives = 372/392 (94.90%), Query Frame = 0


Query: 1   MPQAFSCASRVSKLFYYNSKIFGLSSVVSGSGHHFRLSSAMKEIKVKGGSNADPHLLNDE 60
           MPQAF  ASRVSK F   SKIFGLSSVVSGSGHH RL SAMKEIKVKGGSNADPHLLNDE
Sbjct: 1   MPQAFYWASRVSKRFCRTSKIFGLSSVVSGSGHHCRLFSAMKEIKVKGGSNADPHLLNDE 60

Query: 61  THAQQLSCANQGCSSSNVQCTPEIEKKYVHRVYDAIAPHFSSTRFAKWPKVASFLTSLPL 120
            HA QLSCANQGCS+SNVQCTPEIEKKYVHRVYDAIAPHFSSTRFAKWPKVASFL+SLP 
Sbjct: 61  AHAPQLSCANQGCSASNVQCTPEIEKKYVHRVYDAIAPHFSSTRFAKWPKVASFLSSLPR 120

Query: 121 GSLILDAGCGNGKYLGFIPNCFFIGCDISAQLIKICNERGHEVLVADAVNLPYRTGFGDA 180
           GSLILDAGCGNGKYLGF  NCFFIGCDIS QLIKICNERGHEVLVADAVNLPYRTGFGDA
Sbjct: 121 GSLILDAGCGNGKYLGFNLNCFFIGCDISPQLIKICNERGHEVLVADAVNLPYRTGFGDA 180

Query: 181 AISIAVLHHLSTENRRKKAIEELIRVVKKGGLVLITVWAVEQEDKSLLTKWMPLSEKYVD 240
           AISIAVLHHLSTENRRKKAIEEL+RVVKKGGLVLITVWAVEQEDKSLLTKWMPLSEKYV+
Sbjct: 181 AISIAVLHHLSTENRRKKAIEELLRVVKKGGLVLITVWAVEQEDKSLLTKWMPLSEKYVE 240

Query: 241 EWVGPGSPRVRSPTSLALESIPEMNENNSGIYLKDSKENFTGSMPENKPLPSQSGNDLAN 300
           EWVGPGSPRVRSP+SLALESIPEMNENNSG YLKDSK NFTGSMP N PLPSQSGNDLAN
Sbjct: 241 EWVGPGSPRVRSPSSLALESIPEMNENNSGTYLKDSKVNFTGSMPGNIPLPSQSGNDLAN 300

Query: 301 CNDENLLKTQQEYFVPWHLPYHRAEVSGTSASALASGLAKKDDKKAAVVYNRYYHVFSEG 360
           CNDENL KTQQEYFVPWHLPYHRAEVSGTSA ALASGLAKKDDKKAAVVYNRYYHVFSEG
Sbjct: 301 CNDENLWKTQQEYFVPWHLPYHRAEVSGTSAGALASGLAKKDDKKAAVVYNRYYHVFSEG 360

Query: 361 ELERLVSGMDNAVVVDRFYDKSNWCIVLEKTV 393
           ELERLVSGMDNAVVVDRFYDKSNWCIVLEKT+
Sbjct: 361 ELERLVSGMDNAVVVDRFYDKSNWCIVLEKTM 392

[Close]

BLAST of Lag0030408 vs. NCBI nr
Match: KAG6578341.1 (tRNA (carboxymethyluridine(34)-5-O)-methyltransferase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 738.0 bits (1904), Expect = 4.1e-209
Identity = 366/392 (93.37%), Postives = 372/392 (94.90%), Query Frame = 0


Query: 1   MPQAFSCASRVSKLFYYNSKIFGLSSVVSGSGHHFRLSSAMKEIKVKGGSNADPHLLNDE 60
           MPQAF  ASRVSK F   SKIFGLSSVVSGSGHH RL SAMKEIKVKGGSNADPHLLNDE
Sbjct: 22  MPQAFYWASRVSKRFCRTSKIFGLSSVVSGSGHHCRLFSAMKEIKVKGGSNADPHLLNDE 81

Query: 61  THAQQLSCANQGCSSSNVQCTPEIEKKYVHRVYDAIAPHFSSTRFAKWPKVASFLTSLPL 120
            HA QLSCANQGCS+SNVQCTPEIEKKYVHRVYDAIAPHFSSTRFAKWPKVASFL+SLP 
Sbjct: 82  AHAPQLSCANQGCSASNVQCTPEIEKKYVHRVYDAIAPHFSSTRFAKWPKVASFLSSLPR 141

Query: 121 GSLILDAGCGNGKYLGFIPNCFFIGCDISAQLIKICNERGHEVLVADAVNLPYRTGFGDA 180
           GSLILDAGCGNGKYLGF  NCFFIGCDIS QLIKICNERGHEVLVADAVNLPYRTGFGDA
Sbjct: 142 GSLILDAGCGNGKYLGFNLNCFFIGCDISPQLIKICNERGHEVLVADAVNLPYRTGFGDA 201

Query: 181 AISIAVLHHLSTENRRKKAIEELIRVVKKGGLVLITVWAVEQEDKSLLTKWMPLSEKYVD 240
           AISIAVLHHLSTENRRKKAIEEL+RVVKKGGLVLITVWAVEQEDKSLLTKWMPLSEKYV+
Sbjct: 202 AISIAVLHHLSTENRRKKAIEELLRVVKKGGLVLITVWAVEQEDKSLLTKWMPLSEKYVE 261

Query: 241 EWVGPGSPRVRSPTSLALESIPEMNENNSGIYLKDSKENFTGSMPENKPLPSQSGNDLAN 300
           EWVGPGSPRVRSP+SLALESIPEMNENNSG YLKDSK NFTGSMP N PLPSQSGNDLAN
Sbjct: 262 EWVGPGSPRVRSPSSLALESIPEMNENNSGTYLKDSKVNFTGSMPGNIPLPSQSGNDLAN 321

Query: 301 CNDENLLKTQQEYFVPWHLPYHRAEVSGTSASALASGLAKKDDKKAAVVYNRYYHVFSEG 360
           CNDENL KTQQEYFVPWHLPYHRAEVSGTSA ALASGLAKKDDKKAAVVYNRYYHVFSEG
Sbjct: 322 CNDENLWKTQQEYFVPWHLPYHRAEVSGTSAGALASGLAKKDDKKAAVVYNRYYHVFSEG 381

Query: 361 ELERLVSGMDNAVVVDRFYDKSNWCIVLEKTV 393
           ELERLVSGMDNAVVVDRFYDKSNWCIVLEKT+
Sbjct: 382 ELERLVSGMDNAVVVDRFYDKSNWCIVLEKTM 413

[Close]

BLAST of Lag0030408 vs. NCBI nr
Match: KAG7015918.1 (Alkylated DNA repair protein alkB-like 8, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 737.6 bits (1903), Expect = 5.4e-209
Identity = 366/391 (93.61%), Postives = 371/391 (94.88%), Query Frame = 0


Query: 1   MPQAFSCASRVSKLFYYNSKIFGLSSVVSGSGHHFRLSSAMKEIKVKGGSNADPHLLNDE 60
           MPQAF  ASRVSK F   SKIFGLSSVVSGSGHH RL SAMKEIKVKGGSNADPHLLNDE
Sbjct: 1   MPQAFYWASRVSKRFCRTSKIFGLSSVVSGSGHHCRLFSAMKEIKVKGGSNADPHLLNDE 60

Query: 61  THAQQLSCANQGCSSSNVQCTPEIEKKYVHRVYDAIAPHFSSTRFAKWPKVASFLTSLPL 120
            HA QLSCANQGCS+SNVQCTPEIEKKYVHRVYDAIAPHFSSTRFAKWPKVASFL+SLP 
Sbjct: 61  AHAPQLSCANQGCSASNVQCTPEIEKKYVHRVYDAIAPHFSSTRFAKWPKVASFLSSLPR 120

Query: 121 GSLILDAGCGNGKYLGFIPNCFFIGCDISAQLIKICNERGHEVLVADAVNLPYRTGFGDA 180
           GSLILDAGCGNGKYLGF  NCFFIGCDIS QLIKICNERGHEVLVADAVNLPYRTGFGDA
Sbjct: 121 GSLILDAGCGNGKYLGFNLNCFFIGCDISPQLIKICNERGHEVLVADAVNLPYRTGFGDA 180

Query: 181 AISIAVLHHLSTENRRKKAIEELIRVVKKGGLVLITVWAVEQEDKSLLTKWMPLSEKYVD 240
           AISIAVLHHLSTENRRKKAIEEL+RVVKKGGLVLITVWAVEQEDKSLLTKWMPLSEKYV+
Sbjct: 181 AISIAVLHHLSTENRRKKAIEELLRVVKKGGLVLITVWAVEQEDKSLLTKWMPLSEKYVE 240

Query: 241 EWVGPGSPRVRSPTSLALESIPEMNENNSGIYLKDSKENFTGSMPENKPLPSQSGNDLAN 300
           EWVGPGSPRVRSP+SLALESIPEMNENNSG YLKDSK NFTGSMP N PLPSQSGNDLAN
Sbjct: 241 EWVGPGSPRVRSPSSLALESIPEMNENNSGTYLKDSKVNFTGSMPGNIPLPSQSGNDLAN 300

Query: 301 CNDENLLKTQQEYFVPWHLPYHRAEVSGTSASALASGLAKKDDKKAAVVYNRYYHVFSEG 360
           CNDENL KTQQEYFVPWHLPYHRAEVSGTSA ALASGLAKKDDKKAAVVYNRYYHVFSEG
Sbjct: 301 CNDENLWKTQQEYFVPWHLPYHRAEVSGTSAGALASGLAKKDDKKAAVVYNRYYHVFSEG 360

Query: 361 ELERLVSGMDNAVVVDRFYDKSNWCIVLEKT 392
           ELERLVSGMDNAVVVDRFYDKSNWCIVLEKT
Sbjct: 361 ELERLVSGMDNAVVVDRFYDKSNWCIVLEKT 391

[Close]

BLAST of Lag0030408 vs. NCBI nr
Match: XP_038886423.1 (tRNA (carboxymethyluridine(34)-5-O)-methyltransferase [Benincasa hispida])

HSP 1 Score: 736.1 bits (1899), Expect = 1.6e-208
Identity = 366/393 (93.13%), Postives = 375/393 (95.42%), Query Frame = 0


Query: 1   MPQAFSCASRVSKLFYYNSKIFGLSSVVSGSGHHFRLSSAMKEIKVKGGSNADPHLLNDE 60
           MPQ F  ASRV K F +NSKIFGL SVVSGSGHHFRLSSAMKEIKVKGGSNADPH L +E
Sbjct: 1   MPQDFDRASRVIKRFCHNSKIFGLPSVVSGSGHHFRLSSAMKEIKVKGGSNADPHPLENE 60

Query: 61  THAQQLSCANQGCSSSNVQCTPEIEKKYVHRVYDAIAPHFSSTRFAKWPKVASFLTSLPL 120
            HAQQLSCANQGCS+SNVQCTPEIEKKYVHRVYDAIAPHFSSTRFAKWPKVASFL+SLPL
Sbjct: 61  AHAQQLSCANQGCSASNVQCTPEIEKKYVHRVYDAIAPHFSSTRFAKWPKVASFLSSLPL 120

Query: 121 GSLILDAGCGNGKYLGFIPNCFFIGCDISAQLIKICNERGHEVLVADAVNLPYRTGFGDA 180
           GSL+LDAGCGNGKYLG+ PNCFFIGCDISAQLIKICNERGHEVLVADAVNLPYRTGFGDA
Sbjct: 121 GSLVLDAGCGNGKYLGYNPNCFFIGCDISAQLIKICNERGHEVLVADAVNLPYRTGFGDA 180

Query: 181 AISIAVLHHLSTENRRKKAIEELIRVVKKGGLVLITVWAVEQEDKSLLTKWMPLSEKYVD 240
           AISIAVLHHLSTENRRKKAIEELIRVVKKGGLVLITVWAVEQEDKSLLTKWMPLSEKYV+
Sbjct: 181 AISIAVLHHLSTENRRKKAIEELIRVVKKGGLVLITVWAVEQEDKSLLTKWMPLSEKYVE 240

Query: 241 EWVGPGSPRVRSPTSLALESIPEMNENNSGIYLK-DSKENFTGSMPENKPLPSQSGNDLA 300
           EWVGPGSPRVRSPTSLALESIPEMNENNSGI L+ DSKENFTGS+PE K LPSQ  NDLA
Sbjct: 241 EWVGPGSPRVRSPTSLALESIPEMNENNSGICLRGDSKENFTGSIPEEKLLPSQCENDLA 300

Query: 301 NCNDENLLKTQQEYFVPWHLPYHRAEVSGTSASALASGLAKKDDKKAAVVYNRYYHVFSE 360
           NCN ENLLKTQQEYFVPWHLPYHRAEVSGTSASALASGLAKKDDKKAAVVYNRYYHVFSE
Sbjct: 301 NCNHENLLKTQQEYFVPWHLPYHRAEVSGTSASALASGLAKKDDKKAAVVYNRYYHVFSE 360

Query: 361 GELERLVSGMDNAVVVDRFYDKSNWCIVLEKTV 393
           GELERLVSGMDNAVVVDRFYDKSNWCIVLEKTV
Sbjct: 361 GELERLVSGMDNAVVVDRFYDKSNWCIVLEKTV 393

[Close]

BLAST of Lag0030408 vs. ExPASy Swiss-Prot
Match: Q94A09 (tRNA (carboxymethyluridine(34)-5-O)-methyltransferase OS=Arabidopsis thaliana OX=3702 GN=TRM9 PE=1 SV=1)

HSP 1 Score: 470.3 bits (1209), Expect = 2.1e-131
Identity = 252/382 (65.97%), Postives = 288/382 (75.39%), Query Frame = 0


Query: 31  SGHHFRLSSAMKEIKVKGGSNADPHLLNDETHAQQLSCANQGCSS-SNVQCTPEIEKKYV 90
           SG +   +S M++IKVK  S++   L + +   + +S      SS S+V+ TPEIEKKYV
Sbjct: 19  SGFYSISTSIMRDIKVK--SDSKEFLTSSDEEEESVSIRVSSSSSLSSVKSTPEIEKKYV 78

Query: 91  HRVYDAIAPHFSSTRFAKWPKVASFLTSLPLGSLILDAGCGNGKYLGFIPNCFFIGCDIS 150
           HRVYDAIAPHFSSTRFAKWPKVA+FL SLP GS+ILDAGCGNGKYLG  P+CFFIGCDIS
Sbjct: 79  HRVYDAIAPHFSSTRFAKWPKVAAFLESLPSGSVILDAGCGNGKYLGLNPSCFFIGCDIS 138

Query: 151 AQLIKICNERGHEVLVADAVNLPYRTGFGDAAISIAVLHHLSTENRRKKAIEELIRVVKK 210
             LIKIC+++G EVLVADAVNLPYR  FGDAAISIAVLHHLSTENRRKKAIEEL+RVVK 
Sbjct: 139 HPLIKICSDKGQEVLVADAVNLPYREEFGDAAISIAVLHHLSTENRRKKAIEELVRVVKP 198

Query: 211 GGLVLITVWAVEQEDKSLLTKWMPLSEKYVDEWVGPG----SPRVRSPTSLALESIPEMN 270
           GG VLITVWA EQED SLLTKW PLS KYV+EWVGPG    SPRVR+    +LESIPE  
Sbjct: 199 GGFVLITVWAAEQEDTSLLTKWTPLSAKYVEEWVGPGSPMNSPRVRNNPFFSLESIPETE 258

Query: 271 --------ENNSGIYLKDSKENFTGS--------MPENK-PLPSQSGNDLANCNDENLLK 330
                   EN+  I L+   E+   +        +PE K  +  Q        + E L K
Sbjct: 259 VSTKEQKVENSQFIGLESIPESEESTREQKGESIIPETKASIVEQKDEKSVEESLEALKK 318

Query: 331 TQQEYFVPWHLPYHRAEVSGTSASALASGLAKKDDKKAAVVYNRYYHVFSEGELERLVSG 390
           +QQEYFVPWHLPYHRAEVSG SASALASGLAKKDD+K AVVYNRYYHVFSEGELERL SG
Sbjct: 319 SQQEYFVPWHLPYHRAEVSGASASALASGLAKKDDRKGAVVYNRYYHVFSEGELERLASG 378

[Close]

BLAST of Lag0030408 vs. ExPASy Swiss-Prot
Match: Q07G10 (Alkylated DNA repair protein alkB homolog 8 OS=Xenopus tropicalis OX=8364 GN=alkbh8 PE=2 SV=2)

HSP 1 Score: 221.1 bits (562), Expect = 2.2e-56
Identity = 121/308 (39.29%), Postives = 173/308 (56.17%), Query Frame = 0


Query: 84  IEKKYVHRVYDAIAPHFSSTRFAKWPKVASFLTSLPLGSLILDAGCGNGKYLGFIPNCFF 143
           +E++YVH+VYD IA HFSSTR   WPK+  FL SLP GSL+ D GCGNGKYLG   +   
Sbjct: 369 LEREYVHKVYDDIAGHFSSTRHTPWPKIGDFLASLPKGSLVADVGCGNGKYLGVNKDLCM 428

Query: 144 IGCDISAQLIKICNERGHEVLVADAVNLPYRTGFGDAAISIAVLHHLSTENRRKKAIEEL 203
           IGCD S  L+ IC ER  E  V DA+++P+R G  DA ISIAV+HH +TE RR  A++EL
Sbjct: 429 IGCDRSKNLVDICRERTFEAFVCDALSVPFRAGAFDACISIAVIHHFATEERRIAALQEL 488

Query: 204 IRVVKKGGLVLITVWAVEQEDKSLLTKWMPLSEKYVDEWVGPGSPRVRSPTSLALESIPE 263
           IR+++KGG  LI VWA+EQE K   +K++  S+       GP S  +   T+LA+++   
Sbjct: 489 IRLLRKGGKALIYVWALEQEYKKNKSKYLKESKTS----QGPSSDAL--GTNLAVKAPLP 548

Query: 264 MNENNSGIYLKDSKENFTGSMPENKPLPSQSGNDLANCNDENLLKTQQEYFVPWHL-PYH 323
           ++ N +  +                                      Q+  VPWHL P +
Sbjct: 549 IHTNRTSFH-------------------------------------SQDLLVPWHLKPTN 608

Query: 324 RAEVSGTSASALASGLAKKDDKKAAVVYNRYYHVFSEGELERLVSGMDNAVVVDRFYDKS 383
           +++V+  +          + +     VY+R+YHVF EGELE + + + N  V   ++D+ 
Sbjct: 609 KSKVTPENKE------QNEKEHGPDSVYHRFYHVFCEGELEAMCNRLSNVAVQHSYHDQG 627

Query: 384 NWCIVLEK 391
           NWC++LEK
Sbjct: 669 NWCVILEK 627

[Close]

BLAST of Lag0030408 vs. ExPASy Swiss-Prot
Match: Q80Y20 (Alkylated DNA repair protein alkB homolog 8 OS=Mus musculus OX=10090 GN=Alkbh8 PE=1 SV=1)

HSP 1 Score: 217.6 bits (553), Expect = 2.5e-55
Identity = 121/313 (38.66%), Postives = 179/313 (57.19%), Query Frame = 0


Query: 83  EIEKKYVHRVYDAIAPHFSSTRFAKWPKVASFLTSLPLGSLILDAGCGNGKYLGFIPNCF 142
           E+E+K+VH+VY+ IA HFSSTR + WP++  FL +LP GS++ D GCGNGKYLG   + +
Sbjct: 369 ELEQKHVHQVYNEIASHFSSTRHSPWPRIVEFLKALPSGSIVADIGCGNGKYLGINKDLY 428

Query: 143 FIGCDISAQLIKICNERGHEVLVADAVNLPYRTGFGDAAISIAVLHHLSTENRRKKAIEE 202
            IGCD S  L+ IC ER  + LV DA+ +P R+G  DA ISIAV+HH +T  RR +A++E
Sbjct: 429 MIGCDRSQNLVDICRERQFQALVCDALAVPVRSGSCDACISIAVIHHFATAERRVEALQE 488

Query: 203 LIRVVKKGGLVLITVWAVEQEDKSLLTKWM---PLSEKYVDEWVGPGSPR--VRSPTSLA 262
           L R+++ GG  LI VWA+EQE K+  +K++    +S+   DE     S    + + T   
Sbjct: 489 LARLLRPGGQALIYVWAMEQEYKNQKSKYLRGKRISQGDKDELNSATSTEEFLVNQTPEG 548

Query: 263 LESIPEMNENNSGIYLKDSKENFTGSMPENKPLPSQSGNDLANCNDENLLKTQQEYFVPW 322
           +   P ++ N+S I     +E +      N  LP        +          Q+  VPW
Sbjct: 549 VNEDPALSVNSSSI---TKEEEYKSRKVPNSELPIHINRTCFH---------SQDVLVPW 608

Query: 323 HLPYHRAEVSGTSASALASGLAKKDDKKAAVVYNRYYHVFSEGELERLVSGMDNAVVVDR 382
           HL  +     G   +   SG+A   D   + V++RYYHVF +GELE     + +  ++  
Sbjct: 609 HLKRN----PGKDKAIEPSGVAGCPD--PSPVFHRYYHVFCDGELEASCQAVGDVSILQS 663

Query: 383 FYDKSNWCIVLEK 391
           +YD+ NWC+VL+K
Sbjct: 669 YYDQGNWCVVLQK 663

[Close]

BLAST of Lag0030408 vs. ExPASy Swiss-Prot
Match: A1A4L5 (Alkylated DNA repair protein alkB homolog 8 OS=Bos taurus OX=9913 GN=ALKBH8 PE=2 SV=1)

HSP 1 Score: 209.9 bits (533), Expect = 5.1e-53
Identity = 116/313 (37.06%), Postives = 166/313 (53.04%), Query Frame = 0


Query: 83  EIEKKYVHRVYDAIAPHFSSTRFAKWPKVASFLTSLPLGSLILDAGCGNGKYLGFIPNCF 142
           ++E+ YVHRVY+ IA HFSSTR   WP +  FL +LP GSL+ D GCGNGKYLG     +
Sbjct: 369 QLEQDYVHRVYEEIARHFSSTRHTPWPHIVEFLKALPSGSLVADIGCGNGKYLGINNELY 428

Query: 143 FIGCDISAQLIKICNERGHEVLVADAVNLPYRTGFGDAAISIAVLHHLSTENRRKKAIEE 202
            IGCD S  L+ IC ER ++  V DA+ +P R+G  DA ISIAV+HH +T  RR  A++E
Sbjct: 429 MIGCDHSQNLVDICRERQYQAFVCDALAVPIRSGSCDACISIAVIHHFATAERRVAALQE 488

Query: 203 LIRVVKKGGLVLITVWAVEQEDKSLLTKWMPLSEKYVDEWVGPGSPRVRSPTSLALESIP 262
           L+R+++ GG  LI VWA+EQE     +K+  L E    + +        S   L ++ +P
Sbjct: 489 LVRLLRPGGKALIYVWAMEQEYNKKKSKY--LRENRTSQGMKEEISNDASVQELLVKQLP 548

Query: 263 EMNENNSGIYLKDSKENFTGSMPENKPLPSQSGNDLANCNDENLLKTQQEYFVPWHLPYH 322
            +  N    +   S  +F      +K +     N     +        Q+  VPWH   +
Sbjct: 549 NVG-NQDSAHSVSSINDFQDGGCNSKNV----ANSKLPIHTNRTSFHSQDLLVPWHFKGN 608

Query: 323 -----RAEVSGTSASALASGLAKKDDKKAAVVYNRYYHVFSEGELERLVSGMDNAVVVDR 382
                R E  G   S           +    V++RYYHVF EGELE     ++N  ++  
Sbjct: 609 PGKDKRVEPFGPLGS-----------RDPGPVFHRYYHVFCEGELEAACRTLNNISILQS 663

Query: 383 FYDKSNWCIVLEK 391
           +YD+ NWC++L+K
Sbjct: 669 YYDQGNWCVILQK 663

[Close]

BLAST of Lag0030408 vs. ExPASy Swiss-Prot
Match: Q95K79 (Alkylated DNA repair protein alkB homolog 8 OS=Macaca fascicularis OX=9541 GN=ALKBH8 PE=2 SV=1)

HSP 1 Score: 205.7 bits (522), Expect = 9.7e-52
Identity = 112/313 (35.78%), Postives = 166/313 (53.04%), Query Frame = 0


Query: 84  IEKKYVHRVYDAIAPHFSSTRFAKWPKVASFLTSLPLGSLILDAGCGNGKYLGFIPNCFF 143
           +E++YVH+VY+ IA HFSSTR   WP +  FL +LP GS++ D GCGNGKYLG     + 
Sbjct: 370 LEQEYVHQVYEEIAGHFSSTRHTPWPHIVEFLKALPSGSIVADIGCGNGKYLGVNKELYM 429

Query: 144 IGCDISAQLIKICNERGHEVLVADAVNLPYRTGFGDAAISIAVLHHLSTENRRKKAIEEL 203
           +GCD S  L+ IC ER  +  V DA+ +P R+G  DA ISIAV+HH +T  RR  A++E+
Sbjct: 430 VGCDRSQNLVDICRERQFQAFVCDALAVPVRSGSCDACISIAVIHHFATAERRVAALQEI 489

Query: 204 IRVVKKGGLVLITVWAVEQEDKSLLTKWMPLSEKYVDEWVGPGSPRVRSPTSL---ALES 263
           +R+++ GG  LI VWA+EQE     +K++  +     +        + S TS+    +E 
Sbjct: 490 VRLLRPGGKALIYVWAMEQEYNKQKSKYLKGNRNSQGK-----KEEMNSDTSVQRSLVEQ 549

Query: 264 IPEMNENNSGI---YLKDSKENFTGSMPENKPLPSQSGNDLANCNDENLLKTQQEYFVPW 323
           +P+M   +S      + DS+E    S         Q  N     +        Q+  VPW
Sbjct: 550 MPDMGSRDSASSVPRINDSQEGGCNS--------RQVSNSKLPIHVNRTSFYSQDMLVPW 609

Query: 324 HLPYHRAEVSGTSASALASGLAKKDDKKAAVVYNRYYHVFSEGELERLVSGMDNAVVVDR 383
           HL                        +  + V++RYYHVF EGELE L   + +  ++  
Sbjct: 610 HLK------GNPDKGKPVEPFGPIGSQDPSPVFHRYYHVFREGELEALCRTVSDVRILQS 663

Query: 384 FYDKSNWCIVLEK 391
           +YD+ NWC++L+K
Sbjct: 670 YYDQGNWCVILQK 663

[Close]

BLAST of Lag0030408 vs. ExPASy TrEMBL
Match: A0A6J1FLV5 (alkylated DNA repair protein alkB homolog 8-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111445406 PE=4 SV=1)

HSP 1 Score: 738.0 bits (1904), Expect = 2.0e-209
Identity = 366/392 (93.37%), Postives = 372/392 (94.90%), Query Frame = 0


Query: 1   MPQAFSCASRVSKLFYYNSKIFGLSSVVSGSGHHFRLSSAMKEIKVKGGSNADPHLLNDE 60
           MPQAF  ASRVSK F   SKIFGLSSVVSGSGHH RL SAMKEIKVKGGSNADPHLLNDE
Sbjct: 1   MPQAFYWASRVSKRFCRTSKIFGLSSVVSGSGHHCRLFSAMKEIKVKGGSNADPHLLNDE 60

Query: 61  THAQQLSCANQGCSSSNVQCTPEIEKKYVHRVYDAIAPHFSSTRFAKWPKVASFLTSLPL 120
            HA QLSCANQGCS+SNVQCTPEIEKKYVHRVYDAIAPHFSSTRFAKWPKVASFL+SLP 
Sbjct: 61  AHAPQLSCANQGCSASNVQCTPEIEKKYVHRVYDAIAPHFSSTRFAKWPKVASFLSSLPR 120

Query: 121 GSLILDAGCGNGKYLGFIPNCFFIGCDISAQLIKICNERGHEVLVADAVNLPYRTGFGDA 180
           GSLILDAGCGNGKYLGF  NCFFIGCDIS QLIKICNERGHEVLVADAVNLPYRTGFGDA
Sbjct: 121 GSLILDAGCGNGKYLGFNLNCFFIGCDISPQLIKICNERGHEVLVADAVNLPYRTGFGDA 180

Query: 181 AISIAVLHHLSTENRRKKAIEELIRVVKKGGLVLITVWAVEQEDKSLLTKWMPLSEKYVD 240
           AISIAVLHHLSTENRRKKAIEEL+RVVKKGGLVLITVWAVEQEDKSLLTKWMPLSEKYV+
Sbjct: 181 AISIAVLHHLSTENRRKKAIEELLRVVKKGGLVLITVWAVEQEDKSLLTKWMPLSEKYVE 240

Query: 241 EWVGPGSPRVRSPTSLALESIPEMNENNSGIYLKDSKENFTGSMPENKPLPSQSGNDLAN 300
           EWVGPGSPRVRSP+SLALESIPEMNENNSG YLKDSK NFTGSMP N PLPSQSGNDLAN
Sbjct: 241 EWVGPGSPRVRSPSSLALESIPEMNENNSGTYLKDSKVNFTGSMPGNIPLPSQSGNDLAN 300

Query: 301 CNDENLLKTQQEYFVPWHLPYHRAEVSGTSASALASGLAKKDDKKAAVVYNRYYHVFSEG 360
           CNDENL KTQQEYFVPWHLPYHRAEVSGTSA ALASGLAKKDDKKAAVVYNRYYHVFSEG
Sbjct: 301 CNDENLWKTQQEYFVPWHLPYHRAEVSGTSAGALASGLAKKDDKKAAVVYNRYYHVFSEG 360

Query: 361 ELERLVSGMDNAVVVDRFYDKSNWCIVLEKTV 393
           ELERLVSGMDNAVVVDRFYDKSNWCIVLEKT+
Sbjct: 361 ELERLVSGMDNAVVVDRFYDKSNWCIVLEKTM 392

[Close]

BLAST of Lag0030408 vs. ExPASy TrEMBL
Match: A0A6J1FG55 (alkylated DNA repair protein alkB homolog 8-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111445406 PE=4 SV=1)

HSP 1 Score: 738.0 bits (1904), Expect = 2.0e-209
Identity = 366/392 (93.37%), Postives = 372/392 (94.90%), Query Frame = 0


Query: 1   MPQAFSCASRVSKLFYYNSKIFGLSSVVSGSGHHFRLSSAMKEIKVKGGSNADPHLLNDE 60
           MPQAF  ASRVSK F   SKIFGLSSVVSGSGHH RL SAMKEIKVKGGSNADPHLLNDE
Sbjct: 54  MPQAFYWASRVSKRFCRTSKIFGLSSVVSGSGHHCRLFSAMKEIKVKGGSNADPHLLNDE 113

Query: 61  THAQQLSCANQGCSSSNVQCTPEIEKKYVHRVYDAIAPHFSSTRFAKWPKVASFLTSLPL 120
            HA QLSCANQGCS+SNVQCTPEIEKKYVHRVYDAIAPHFSSTRFAKWPKVASFL+SLP 
Sbjct: 114 AHAPQLSCANQGCSASNVQCTPEIEKKYVHRVYDAIAPHFSSTRFAKWPKVASFLSSLPR 173

Query: 121 GSLILDAGCGNGKYLGFIPNCFFIGCDISAQLIKICNERGHEVLVADAVNLPYRTGFGDA 180
           GSLILDAGCGNGKYLGF  NCFFIGCDIS QLIKICNERGHEVLVADAVNLPYRTGFGDA
Sbjct: 174 GSLILDAGCGNGKYLGFNLNCFFIGCDISPQLIKICNERGHEVLVADAVNLPYRTGFGDA 233

Query: 181 AISIAVLHHLSTENRRKKAIEELIRVVKKGGLVLITVWAVEQEDKSLLTKWMPLSEKYVD 240
           AISIAVLHHLSTENRRKKAIEEL+RVVKKGGLVLITVWAVEQEDKSLLTKWMPLSEKYV+
Sbjct: 234 AISIAVLHHLSTENRRKKAIEELLRVVKKGGLVLITVWAVEQEDKSLLTKWMPLSEKYVE 293

Query: 241 EWVGPGSPRVRSPTSLALESIPEMNENNSGIYLKDSKENFTGSMPENKPLPSQSGNDLAN 300
           EWVGPGSPRVRSP+SLALESIPEMNENNSG YLKDSK NFTGSMP N PLPSQSGNDLAN
Sbjct: 294 EWVGPGSPRVRSPSSLALESIPEMNENNSGTYLKDSKVNFTGSMPGNIPLPSQSGNDLAN 353

Query: 301 CNDENLLKTQQEYFVPWHLPYHRAEVSGTSASALASGLAKKDDKKAAVVYNRYYHVFSEG 360
           CNDENL KTQQEYFVPWHLPYHRAEVSGTSA ALASGLAKKDDKKAAVVYNRYYHVFSEG
Sbjct: 354 CNDENLWKTQQEYFVPWHLPYHRAEVSGTSAGALASGLAKKDDKKAAVVYNRYYHVFSEG 413

Query: 361 ELERLVSGMDNAVVVDRFYDKSNWCIVLEKTV 393
           ELERLVSGMDNAVVVDRFYDKSNWCIVLEKT+
Sbjct: 414 ELERLVSGMDNAVVVDRFYDKSNWCIVLEKTM 445

[Close]

BLAST of Lag0030408 vs. ExPASy TrEMBL
Match: A0A6J1JY21 (alkylated DNA repair protein alkB homolog 8-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111489322 PE=4 SV=1)

HSP 1 Score: 735.7 bits (1898), Expect = 9.9e-209
Identity = 364/392 (92.86%), Postives = 371/392 (94.64%), Query Frame = 0


Query: 1   MPQAFSCASRVSKLFYYNSKIFGLSSVVSGSGHHFRLSSAMKEIKVKGGSNADPHLLNDE 60
           MPQAF  ASRVSK F + SKIFGLSSVVSGS HH RL SAMKEIKVKGGSNADPHLLNDE
Sbjct: 1   MPQAFYWASRVSKRFCHTSKIFGLSSVVSGSVHHCRLFSAMKEIKVKGGSNADPHLLNDE 60

Query: 61  THAQQLSCANQGCSSSNVQCTPEIEKKYVHRVYDAIAPHFSSTRFAKWPKVASFLTSLPL 120
            HA QLSCANQGCS+SNVQCTPEIEKKYVHRVYDAIAPHFSSTRFAKWPKVASFL+SLP 
Sbjct: 61  AHAPQLSCANQGCSASNVQCTPEIEKKYVHRVYDAIAPHFSSTRFAKWPKVASFLSSLPR 120

Query: 121 GSLILDAGCGNGKYLGFIPNCFFIGCDISAQLIKICNERGHEVLVADAVNLPYRTGFGDA 180
           GSLILDAGCGNGKYLGF  NCFFIGCDIS QLIKICNERGHEVLVADAVNLPYRTGFGDA
Sbjct: 121 GSLILDAGCGNGKYLGFNLNCFFIGCDISPQLIKICNERGHEVLVADAVNLPYRTGFGDA 180

Query: 181 AISIAVLHHLSTENRRKKAIEELIRVVKKGGLVLITVWAVEQEDKSLLTKWMPLSEKYVD 240
           AISIAVLHHLSTENRRKKAIEEL+RVVKKGGLVLITVWAVEQEDKSLLTKWMPLSEKYV+
Sbjct: 181 AISIAVLHHLSTENRRKKAIEELLRVVKKGGLVLITVWAVEQEDKSLLTKWMPLSEKYVE 240

Query: 241 EWVGPGSPRVRSPTSLALESIPEMNENNSGIYLKDSKENFTGSMPENKPLPSQSGNDLAN 300
           EWVGPGSPRVRSP+SLALESIPEMNENNSG YLKDSK NFTGSMPEN PLPSQ GND AN
Sbjct: 241 EWVGPGSPRVRSPSSLALESIPEMNENNSGTYLKDSKVNFTGSMPENIPLPSQGGNDFAN 300

Query: 301 CNDENLLKTQQEYFVPWHLPYHRAEVSGTSASALASGLAKKDDKKAAVVYNRYYHVFSEG 360
           CNDENL KTQQEYFVPWHLPYHRAEVSGTSA ALASGLAKKDDKKAAVVYNRYYHVFSEG
Sbjct: 301 CNDENLWKTQQEYFVPWHLPYHRAEVSGTSAGALASGLAKKDDKKAAVVYNRYYHVFSEG 360

Query: 361 ELERLVSGMDNAVVVDRFYDKSNWCIVLEKTV 393
           ELERLVSGMDNAVVVDRFYDKSNWCIVLEKT+
Sbjct: 361 ELERLVSGMDNAVVVDRFYDKSNWCIVLEKTM 392

[Close]

BLAST of Lag0030408 vs. ExPASy TrEMBL
Match: A0A6J1K1M9 (alkylated DNA repair protein alkB homolog 8-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111489322 PE=4 SV=1)

HSP 1 Score: 735.7 bits (1898), Expect = 9.9e-209
Identity = 364/392 (92.86%), Postives = 371/392 (94.64%), Query Frame = 0


Query: 1   MPQAFSCASRVSKLFYYNSKIFGLSSVVSGSGHHFRLSSAMKEIKVKGGSNADPHLLNDE 60
           MPQAF  ASRVSK F + SKIFGLSSVVSGS HH RL SAMKEIKVKGGSNADPHLLNDE
Sbjct: 54  MPQAFYWASRVSKRFCHTSKIFGLSSVVSGSVHHCRLFSAMKEIKVKGGSNADPHLLNDE 113

Query: 61  THAQQLSCANQGCSSSNVQCTPEIEKKYVHRVYDAIAPHFSSTRFAKWPKVASFLTSLPL 120
            HA QLSCANQGCS+SNVQCTPEIEKKYVHRVYDAIAPHFSSTRFAKWPKVASFL+SLP 
Sbjct: 114 AHAPQLSCANQGCSASNVQCTPEIEKKYVHRVYDAIAPHFSSTRFAKWPKVASFLSSLPR 173

Query: 121 GSLILDAGCGNGKYLGFIPNCFFIGCDISAQLIKICNERGHEVLVADAVNLPYRTGFGDA 180
           GSLILDAGCGNGKYLGF  NCFFIGCDIS QLIKICNERGHEVLVADAVNLPYRTGFGDA
Sbjct: 174 GSLILDAGCGNGKYLGFNLNCFFIGCDISPQLIKICNERGHEVLVADAVNLPYRTGFGDA 233

Query: 181 AISIAVLHHLSTENRRKKAIEELIRVVKKGGLVLITVWAVEQEDKSLLTKWMPLSEKYVD 240
           AISIAVLHHLSTENRRKKAIEEL+RVVKKGGLVLITVWAVEQEDKSLLTKWMPLSEKYV+
Sbjct: 234 AISIAVLHHLSTENRRKKAIEELLRVVKKGGLVLITVWAVEQEDKSLLTKWMPLSEKYVE 293

Query: 241 EWVGPGSPRVRSPTSLALESIPEMNENNSGIYLKDSKENFTGSMPENKPLPSQSGNDLAN 300
           EWVGPGSPRVRSP+SLALESIPEMNENNSG YLKDSK NFTGSMPEN PLPSQ GND AN
Sbjct: 294 EWVGPGSPRVRSPSSLALESIPEMNENNSGTYLKDSKVNFTGSMPENIPLPSQGGNDFAN 353

Query: 301 CNDENLLKTQQEYFVPWHLPYHRAEVSGTSASALASGLAKKDDKKAAVVYNRYYHVFSEG 360
           CNDENL KTQQEYFVPWHLPYHRAEVSGTSA ALASGLAKKDDKKAAVVYNRYYHVFSEG
Sbjct: 354 CNDENLWKTQQEYFVPWHLPYHRAEVSGTSAGALASGLAKKDDKKAAVVYNRYYHVFSEG 413

Query: 361 ELERLVSGMDNAVVVDRFYDKSNWCIVLEKTV 393
           ELERLVSGMDNAVVVDRFYDKSNWCIVLEKT+
Sbjct: 414 ELERLVSGMDNAVVVDRFYDKSNWCIVLEKTM 445

[Close]

BLAST of Lag0030408 vs. ExPASy TrEMBL
Match: A0A0A0LMT8 (Methyltransf_11 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G403730 PE=4 SV=1)

HSP 1 Score: 722.2 bits (1863), Expect = 1.1e-204
Identity = 357/392 (91.07%), Postives = 373/392 (95.15%), Query Frame = 0


Query: 1   MPQAFSCASRVSKLFYYNSKIFGLSSVVSGSGHHFRLSSAMKEIKVKGGSNADPHLLNDE 60
           M Q+F  +SRVSK F ++ KIFGLSSVV GSGHHFRL+SAMKEIK+KGGSNADPH LNDE
Sbjct: 1   MLQSFDRSSRVSKYFCHSCKIFGLSSVVIGSGHHFRLNSAMKEIKIKGGSNADPHPLNDE 60

Query: 61  THAQQLSCANQGCSSSNVQCTPEIEKKYVHRVYDAIAPHFSSTRFAKWPKVASFLTSLPL 120
           THA QLSCA QGC++SNVQCTPEIEKKYVHRVYDAIAPHFSSTRFAKWPKVASFL+SLPL
Sbjct: 61  THA-QLSCAIQGCTASNVQCTPEIEKKYVHRVYDAIAPHFSSTRFAKWPKVASFLSSLPL 120

Query: 121 GSLILDAGCGNGKYLGFIPNCFFIGCDISAQLIKICNERGHEVLVADAVNLPYRTGFGDA 180
           GSL+LDAGCGNGKYLG+  NCFFIGCDISAQLIKICNERGHEVLVADAVNLPYRTGFGDA
Sbjct: 121 GSLVLDAGCGNGKYLGYNSNCFFIGCDISAQLIKICNERGHEVLVADAVNLPYRTGFGDA 180

Query: 181 AISIAVLHHLSTENRRKKAIEELIRVVKKGGLVLITVWAVEQEDKSLLTKWMPLSEKYVD 240
           AISIAVLHHLSTENRRKKAIEELIRVVKKGGLVLITVWAVEQEDKSLLTKWMPLSEKYV+
Sbjct: 181 AISIAVLHHLSTENRRKKAIEELIRVVKKGGLVLITVWAVEQEDKSLLTKWMPLSEKYVE 240

Query: 241 EWVGPGSPRVRSPTSLALESIPEMNENNSGIYLKDSKENFTGSMPENKPLPSQSGNDLAN 300
           EWVGPGSPRVRSP+S+ALESIPEMNENNSGI LKDSKEN TGS+PENKP PSQS NDLAN
Sbjct: 241 EWVGPGSPRVRSPSSMALESIPEMNENNSGICLKDSKENLTGSIPENKPPPSQSENDLAN 300

Query: 301 CNDENLLKTQQEYFVPWHLPYHRAEVSGTSASALASGLAKKDDKKAAVVYNRYYHVFSEG 360
           CN ENLLKTQQEYFVPWHLPYHRAEVSGTSASALASGLAKKDDKKAAVVYNRYYHVFSEG
Sbjct: 301 CNHENLLKTQQEYFVPWHLPYHRAEVSGTSASALASGLAKKDDKKAAVVYNRYYHVFSEG 360

Query: 361 ELERLVSGMDNAVVVDRFYDKSNWCIVLEKTV 393
           ELE L+SGMDNAVVVDRFYDKSNWCIVLEK V
Sbjct: 361 ELEGLISGMDNAVVVDRFYDKSNWCIVLEKLV 391

[Close]

BLAST of Lag0030408 vs. TAIR 10
Match: AT1G36310.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 470.3 bits (1209), Expect = 1.5e-132
Identity = 252/382 (65.97%), Postives = 288/382 (75.39%), Query Frame = 0


Query: 31  SGHHFRLSSAMKEIKVKGGSNADPHLLNDETHAQQLSCANQGCSS-SNVQCTPEIEKKYV 90
           SG +   +S M++IKVK  S++   L + +   + +S      SS S+V+ TPEIEKKYV
Sbjct: 19  SGFYSISTSIMRDIKVK--SDSKEFLTSSDEEEESVSIRVSSSSSLSSVKSTPEIEKKYV 78

Query: 91  HRVYDAIAPHFSSTRFAKWPKVASFLTSLPLGSLILDAGCGNGKYLGFIPNCFFIGCDIS 150
           HRVYDAIAPHFSSTRFAKWPKVA+FL SLP GS+ILDAGCGNGKYLG  P+CFFIGCDIS
Sbjct: 79  HRVYDAIAPHFSSTRFAKWPKVAAFLESLPSGSVILDAGCGNGKYLGLNPSCFFIGCDIS 138

Query: 151 AQLIKICNERGHEVLVADAVNLPYRTGFGDAAISIAVLHHLSTENRRKKAIEELIRVVKK 210
             LIKIC+++G EVLVADAVNLPYR  FGDAAISIAVLHHLSTENRRKKAIEEL+RVVK 
Sbjct: 139 HPLIKICSDKGQEVLVADAVNLPYREEFGDAAISIAVLHHLSTENRRKKAIEELVRVVKP 198

Query: 211 GGLVLITVWAVEQEDKSLLTKWMPLSEKYVDEWVGPG----SPRVRSPTSLALESIPEMN 270
           GG VLITVWA EQED SLLTKW PLS KYV+EWVGPG    SPRVR+    +LESIPE  
Sbjct: 199 GGFVLITVWAAEQEDTSLLTKWTPLSAKYVEEWVGPGSPMNSPRVRNNPFFSLESIPETE 258

Query: 271 --------ENNSGIYLKDSKENFTGS--------MPENK-PLPSQSGNDLANCNDENLLK 330
                   EN+  I L+   E+   +        +PE K  +  Q        + E L K
Sbjct: 259 VSTKEQKVENSQFIGLESIPESEESTREQKGESIIPETKASIVEQKDEKSVEESLEALKK 318

Query: 331 TQQEYFVPWHLPYHRAEVSGTSASALASGLAKKDDKKAAVVYNRYYHVFSEGELERLVSG 390
           +QQEYFVPWHLPYHRAEVSG SASALASGLAKKDD+K AVVYNRYYHVFSEGELERL SG
Sbjct: 319 SQQEYFVPWHLPYHRAEVSGASASALASGLAKKDDRKGAVVYNRYYHVFSEGELERLASG 378

[Close]

BLAST of Lag0030408 vs. TAIR 10
Match: AT1G36310.2 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 470.3 bits (1209), Expect = 1.5e-132
Identity = 252/382 (65.97%), Postives = 288/382 (75.39%), Query Frame = 0


Query: 31  SGHHFRLSSAMKEIKVKGGSNADPHLLNDETHAQQLSCANQGCSS-SNVQCTPEIEKKYV 90
           SG +   +S M++IKVK  S++   L + +   + +S      SS S+V+ TPEIEKKYV
Sbjct: 19  SGFYSISTSIMRDIKVK--SDSKEFLTSSDEEEESVSIRVSSSSSLSSVKSTPEIEKKYV 78

Query: 91  HRVYDAIAPHFSSTRFAKWPKVASFLTSLPLGSLILDAGCGNGKYLGFIPNCFFIGCDIS 150
           HRVYDAIAPHFSSTRFAKWPKVA+FL SLP GS+ILDAGCGNGKYLG  P+CFFIGCDIS
Sbjct: 79  HRVYDAIAPHFSSTRFAKWPKVAAFLESLPSGSVILDAGCGNGKYLGLNPSCFFIGCDIS 138

Query: 151 AQLIKICNERGHEVLVADAVNLPYRTGFGDAAISIAVLHHLSTENRRKKAIEELIRVVKK 210
             LIKIC+++G EVLVADAVNLPYR  FGDAAISIAVLHHLSTENRRKKAIEEL+RVVK 
Sbjct: 139 HPLIKICSDKGQEVLVADAVNLPYREEFGDAAISIAVLHHLSTENRRKKAIEELVRVVKP 198

Query: 211 GGLVLITVWAVEQEDKSLLTKWMPLSEKYVDEWVGPG----SPRVRSPTSLALESIPEMN 270
           GG VLITVWA EQED SLLTKW PLS KYV+EWVGPG    SPRVR+    +LESIPE  
Sbjct: 199 GGFVLITVWAAEQEDTSLLTKWTPLSAKYVEEWVGPGSPMNSPRVRNNPFFSLESIPETE 258

Query: 271 --------ENNSGIYLKDSKENFTGS--------MPENK-PLPSQSGNDLANCNDENLLK 330
                   EN+  I L+   E+   +        +PE K  +  Q        + E L K
Sbjct: 259 VSTKEQKVENSQFIGLESIPESEESTREQKGESIIPETKASIVEQKDEKSVEESLEALKK 318

Query: 331 TQQEYFVPWHLPYHRAEVSGTSASALASGLAKKDDKKAAVVYNRYYHVFSEGELERLVSG 390
           +QQEYFVPWHLPYHRAEVSG SASALASGLAKKDD+K AVVYNRYYHVFSEGELERL SG
Sbjct: 319 SQQEYFVPWHLPYHRAEVSGASASALASGLAKKDDRKGAVVYNRYYHVFSEGELERLASG 378

The following BLAST results are available for this feature:

BLAST of Lag0030408 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022939521.1	4.1e-209	93.37	alkylated DNA repair protein alkB homolog 8-like isoform X1 [Cucurbita moschata]	[more]
XP_022939523.1	4.1e-209	93.37	alkylated DNA repair protein alkB homolog 8-like isoform X2 [Cucurbita moschata]	[more]
KAG6578341.1	4.1e-209	93.37	tRNA (carboxymethyluridine(34)-5-O)-methyltransferase, partial [Cucurbita argyro...	[more]
KAG7015918.1	5.4e-209	93.61	Alkylated DNA repair protein alkB-like 8, partial [Cucurbita argyrosperma subsp....	[more]
XP_038886423.1	1.6e-208	93.13	tRNA (carboxymethyluridine(34)-5-O)-methyltransferase [Benincasa hispida]	[more]

BLAST of Lag0030408 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q94A09	2.1e-131	65.97	tRNA (carboxymethyluridine(34)-5-O)-methyltransferase OS=Arabidopsis thaliana OX...	[more]
Q07G10	2.2e-56	39.29	Alkylated DNA repair protein alkB homolog 8 OS=Xenopus tropicalis OX=8364 GN=alk...	[more]
Q80Y20	2.5e-55	38.66	Alkylated DNA repair protein alkB homolog 8 OS=Mus musculus OX=10090 GN=Alkbh8 P...	[more]
A1A4L5	5.1e-53	37.06	Alkylated DNA repair protein alkB homolog 8 OS=Bos taurus OX=9913 GN=ALKBH8 PE=2...	[more]
Q95K79	9.7e-52	35.78	Alkylated DNA repair protein alkB homolog 8 OS=Macaca fascicularis OX=9541 GN=AL...	[more]

BLAST of Lag0030408 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FLV5	2.0e-209	93.37	alkylated DNA repair protein alkB homolog 8-like isoform X2 OS=Cucurbita moschat...	[more]
A0A6J1FG55	2.0e-209	93.37	alkylated DNA repair protein alkB homolog 8-like isoform X1 OS=Cucurbita moschat...	[more]
A0A6J1JY21	9.9e-209	92.86	alkylated DNA repair protein alkB homolog 8-like isoform X2 OS=Cucurbita maxima ...	[more]
A0A6J1K1M9	9.9e-209	92.86	alkylated DNA repair protein alkB homolog 8-like isoform X1 OS=Cucurbita maxima ...	[more]
A0A0A0LMT8	1.1e-204	91.07	Methyltransf_11 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G40...	[more]

BLAST of Lag0030408 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G36310.1	1.5e-132	65.97	S-adenosyl-L-methionine-dependent methyltransferases superfamily protein	[more]
AT1G36310.2	1.5e-132	65.97	S-adenosyl-L-methionine-dependent methyltransferases superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR013216	Methyltransferase type 11	PFAM	PF08241	Methyltransf_11	coord: 125..215 e-value: 7.5E-15 score: 55.4
None	No IPR available	GENE3D	3.40.50.150	Vaccinia Virus protein VP39	coord: 100..391 e-value: 1.3E-57 score: 196.5
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 275..298
None	No IPR available	PANTHER	PTHR13069	UNCHARACTERIZED	coord: 74..266
None	No IPR available	PANTHER	PTHR13069:SF33	BNAA05G09210D PROTEIN	coord: 74..266 coord: 306..385
None	No IPR available	PANTHER	PTHR13069	UNCHARACTERIZED	coord: 306..385
None	No IPR available	CDD	cd02440	AdoMet_MTases	coord: 124..220 e-value: 6.27402E-10 score: 54.3583
IPR029063	S-adenosyl-L-methionine-dependent methyltransferase	SUPERFAMILY	53335	S-adenosyl-L-methionine-dependent methyltransferases	coord: 78..239

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0030408.1	Lag0030408.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0032259	methylation
biological_process	GO:0002098	tRNA wobble uridine modification
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0005634	nucleus
molecular_function	GO:0008168	methyltransferase activity
molecular_function	GO:0000049	tRNA binding