Lag0030352 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0030352 vs. NCBI nr
Match: XP_023550775.1 (putative calcium-binding protein CML19 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 200.7 bits (509), Expect = 9.6e-48
Identity = 123/200 (61.50%), Postives = 133/200 (66.50%), Query Frame = 0

Query: 1   MVDHLLHQLTATAIFIYQLRRQIRRSNHGVQA------------------------GRRR 60
           MV HLLH LTA+AIFI QL   IRRSNHG +                         G R 
Sbjct: 1   MVGHLLHDLTASAIFIPQL---IRRSNHGTRIPFHIIADPFCCNPQFFIPKSLSSFGSRM 60

Query: 61  RRREAV---------SSGAAAVH--------GGRRGSLTLQEAEMVVKNLDSDGDGLVGW 120
             + A+           G   +          G  GSLTL+EAEMVV+NLD+DGDGL+GW
Sbjct: 61  MDKMALYEKVFKQVDGDGDGKLSPLELQRCMAGVGGSLTLEEAEMVVENLDTDGDGLMGW 120

Query: 121 AEFVAFVEGVEEEEKVSDLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIA 160
            EFVAFVEGV EEEKVSDLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEEC KMIA
Sbjct: 121 GEFVAFVEGVGEEEKVSDLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECNKMIA 180

BLAST of Lag0030352 vs. NCBI nr
Match: XP_022133861.1 (putative calcium-binding protein CML19 [Momordica charantia])

HSP 1 Score: 198.7 bits (504), Expect = 3.7e-47
Identity = 99/105 (94.29%), Postives = 103/105 (98.10%), Query Frame = 0

Query: 55  GSLTLQEAEMVVKNLDSDGDGLVGWAEFVAFVEGVEEEEKVSDLKEAFRMYEMDGCGFIT 114
           GSLTL+EAE+VV NLDSDGDGLVGWAEFVAFVEGV+EEEKV DLKEAFRMYEMDGCGFIT
Sbjct: 36  GSLTLEEAELVVGNLDSDGDGLVGWAEFVAFVEGVKEEEKVRDLKEAFRMYEMDGCGFIT 95

Query: 115 TKSLKRMLSRLGESRSIEECKKMIAKFDLDSDGVLNFDEFKFMMS 160
           TKSLKRMLSRLGESRSIEECKKMIAKFDL+SDGVLNFDEFKFMMS
Sbjct: 96  TKSLKRMLSRLGESRSIEECKKMIAKFDLNSDGVLNFDEFKFMMS 140

BLAST of Lag0030352 vs. NCBI nr
Match: XP_022955599.1 (putative calcium-binding protein CML19 [Cucurbita moschata] >XP_022955608.1 putative calcium-binding protein CML19 [Cucurbita moschata] >KAG6602674.1 putative calcium-binding protein CML19, partial [Cucurbita argyrosperma subsp. sororia] >KAG7033361.1 putative calcium-binding protein CML19, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 193.7 bits (491), Expect = 1.2e-45
Identity = 97/105 (92.38%), Postives = 101/105 (96.19%), Query Frame = 0

Query: 55  GSLTLQEAEMVVKNLDSDGDGLVGWAEFVAFVEGVEEEEKVSDLKEAFRMYEMDGCGFIT 114
           GSLTL+EAE VV+NLDSDGDGLV WAEFVAF+EGV EEEKVSDL EAFRMYEMDGCGFIT
Sbjct: 36  GSLTLEEAEKVVENLDSDGDGLVSWAEFVAFMEGVGEEEKVSDLMEAFRMYEMDGCGFIT 95

Query: 115 TKSLKRMLSRLGESRSIEECKKMIAKFDLDSDGVLNFDEFKFMMS 160
           TKSLKRMLSRLGESRSIEECKKMIAKFDL+SDGVLNFDEFKFMMS
Sbjct: 96  TKSLKRMLSRLGESRSIEECKKMIAKFDLNSDGVLNFDEFKFMMS 140

BLAST of Lag0030352 vs. NCBI nr
Match: XP_023543960.1 (putative calcium-binding protein CML19 [Cucurbita pepo subsp. pepo] >XP_023543968.1 putative calcium-binding protein CML19 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 193.7 bits (491), Expect = 1.2e-45
Identity = 97/105 (92.38%), Postives = 101/105 (96.19%), Query Frame = 0

Query: 55  GSLTLQEAEMVVKNLDSDGDGLVGWAEFVAFVEGVEEEEKVSDLKEAFRMYEMDGCGFIT 114
           GSLTL+EAE VV+NLDSDGDGLV WAEFVAF+EGV EEEKVSDL EAFRMYEMDGCGFIT
Sbjct: 36  GSLTLEEAEKVVENLDSDGDGLVSWAEFVAFMEGVGEEEKVSDLMEAFRMYEMDGCGFIT 95

Query: 115 TKSLKRMLSRLGESRSIEECKKMIAKFDLDSDGVLNFDEFKFMMS 160
           TKSLKRMLSRLGESRSIEECKKMIAKFDL+SDGVLNFDEFKFMMS
Sbjct: 96  TKSLKRMLSRLGESRSIEECKKMIAKFDLNSDGVLNFDEFKFMMS 140

BLAST of Lag0030352 vs. NCBI nr
Match: XP_022938684.1 (putative calcium-binding protein CML19 [Cucurbita moschata] >XP_023550776.1 putative calcium-binding protein CML19 isoform X2 [Cucurbita pepo subsp. pepo] >KAG7015957.1 putative calcium-binding protein CML19, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 193.4 bits (490), Expect = 1.5e-45
Identity = 97/108 (89.81%), Postives = 102/108 (94.44%), Query Frame = 0

Query: 52  GRRGSLTLQEAEMVVKNLDSDGDGLVGWAEFVAFVEGVEEEEKVSDLKEAFRMYEMDGCG 111
           G  GSLTL+EAEMVV+NLD+DGDGL+GW EFVAFVEGV EEEKVSDLKEAFRMYEMDGCG
Sbjct: 34  GVGGSLTLEEAEMVVENLDTDGDGLMGWGEFVAFVEGVGEEEKVSDLKEAFRMYEMDGCG 93

Query: 112 FITTKSLKRMLSRLGESRSIEECKKMIAKFDLDSDGVLNFDEFKFMMS 160
           FITTKSLKRMLSRLGESRSIEEC KMIAKFDL+SDGVLNFDEF FMMS
Sbjct: 94  FITTKSLKRMLSRLGESRSIEECNKMIAKFDLNSDGVLNFDEFMFMMS 141

BLAST of Lag0030352 vs. ExPASy Swiss-Prot
Match: Q8RYJ8 (Putative calcium-binding protein CML19 OS=Oryza sativa subsp. japonica OX=39947 GN=CML19 PE=3 SV=1)

HSP 1 Score: 107.5 bits (267), Expect = 1.5e-22
Identity = 56/105 (53.33%), Postives = 74/105 (70.48%), Query Frame = 0

Query: 57  LTLQEAEMVVKNLDSDGDGLVGWAEFVAF---VEGVEEEEKVSDLKEAFRMYEMDGCGFI 116
           ++ +EAE +V + D+D DGL+   EF      +E  +EEE+   L EAFRMYEM+G G I
Sbjct: 40  MSAEEAEALVSSADTDDDGLLDEEEFTKLAVQLEMGDEEERCRGLMEAFRMYEMEGEGRI 99

Query: 117 TTKSLKRMLSRLGESRSIEECKKMIAKFDLDSDGVLNFDEFKFMM 159
           T  SLKRMLS+LG  + IEEC+ MI +FDLD DGV++F+EFK MM
Sbjct: 100 TPASLKRMLSKLGSHQGIEECQTMICRFDLDGDGVISFEEFKIMM 144

BLAST of Lag0030352 vs. ExPASy Swiss-Prot
Match: Q8RYK0 (Probable calcium-binding protein CML31 OS=Oryza sativa subsp. japonica OX=39947 GN=CML31 PE=2 SV=1)

HSP 1 Score: 106.3 bits (264), Expect = 3.2e-22
Identity = 55/104 (52.88%), Postives = 75/104 (72.12%), Query Frame = 0

Query: 60  QEAEMVVKNLDSDGDGLVGWAEFVAFVEGVEEEE----KVSDLKEAFRMYEMDGCGFITT 119
           +EA  +V ++D+DGDGL+  AEFV  V+  E EE    + + L+EAF MYEM+G G IT 
Sbjct: 47  EEAGQLVASVDADGDGLLCEAEFVRLVQAAEVEEEDERRGTGLREAFGMYEMEGEGCITP 106

Query: 120 KSLKRMLSRLGESRSIEECKKMIAKFDLDSDGVLNFDEFKFMMS 160
            SL+RML RLG  + I++C+ MI +FDL+ DGVL+FDEFK MM+
Sbjct: 107 TSLRRMLRRLGSDQDIDDCRAMICRFDLNGDGVLSFDEFKIMMN 150

BLAST of Lag0030352 vs. ExPASy Swiss-Prot
Match: Q9FIH9 (Calcium-binding protein CML37 OS=Arabidopsis thaliana OX=3702 GN=CML37 PE=1 SV=1)

HSP 1 Score: 105.5 bits (262), Expect = 5.5e-22
Identity = 56/106 (52.83%), Postives = 74/106 (69.81%), Query Frame = 0

Query: 55  GSLTLQEAEMVVKNLDSDGDGLVGWAEFVAFVEGVE--EEEKVSDLKEAFRMYEMDGCGF 114
           G+L+ +E E VVK  D DGDG + + EF+  +EG +  +EE+  +LKEAF MY M+G  F
Sbjct: 79  GALSSREVEEVVKTSDVDGDGFIDFEEFLKLMEGEDGSDEERRKELKEAFGMYVMEGEEF 138

Query: 115 ITTKSLKRMLSRLGESRSIEECKKMIAKFDLDSDGVLNFDEFKFMM 159
           IT  SL+R LSRLGES +++ CK MI  FD + DGVL+FDEF  MM
Sbjct: 139 ITAASLRRTLSRLGESCTVDACKVMIRGFDQNDDGVLSFDEFVLMM 184

BLAST of Lag0030352 vs. ExPASy Swiss-Prot
Match: Q9SRE6 (Calcium-binding protein CML38 OS=Arabidopsis thaliana OX=3702 GN=CML38 PE=1 SV=1)

HSP 1 Score: 99.4 bits (246), Expect = 4.0e-20
Identity = 50/102 (49.02%), Postives = 73/102 (71.57%), Query Frame = 0

Query: 57  LTLQEAEMVVKNLDSDGDGLVGWAEFVAFVEGVEEEEKVSDLKEAFRMYEMDGCGFITTK 116
           L+ +EA   V+  D+DGDG++ + EF   ++  +EEEK  +LK AFR+Y  +G   IT +
Sbjct: 75  LSDEEAVAAVRLSDTDGDGMLDFEEFSQLIKVDDEEEKKMELKGAFRLYIAEGEDCITPR 134

Query: 117 SLKRMLSRLGESRSIEECKKMIAKFDLDSDGVLNFDEFKFMM 159
           SLK ML +LGESR+ ++C+ MI+ FDL++DGVL+FDEF  MM
Sbjct: 135 SLKMMLKKLGESRTTDDCRVMISAFDLNADGVLSFDEFALMM 176

BLAST of Lag0030352 vs. ExPASy Swiss-Prot
Match: Q9SRE7 (Calcium-binding protein CML39 OS=Arabidopsis thaliana OX=3702 GN=CML39 PE=2 SV=1)

HSP 1 Score: 97.4 bits (241), Expect = 1.5e-19
Identity = 54/102 (52.94%), Postives = 68/102 (66.67%), Query Frame = 0

Query: 60  QEAEMVVKNLDSDGDGLVGWAEFVAFVEGVE---EEEKVSDLKEAFRMYEMDGCGFITTK 119
           +EAE  VK  D DGDG++   EF   ++G +   EEEK   + EAFRMY  DG   IT  
Sbjct: 57  EEAEAAVKLSDIDGDGMLDINEFALLIKGNDEFTEEEKKRKIMEAFRMYIADGEDCITPG 116

Query: 120 SLKRMLSRLGESRSIEECKKMIAKFDLDSDGVLNFDEFKFMM 159
           SLK ML +LGESR+ ++CK MI  FDL++DGVL+FDEF  MM
Sbjct: 117 SLKMMLMKLGESRTTDDCKVMIQAFDLNADGVLSFDEFALMM 158