Lag0030320 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0030320
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionS-adenosyl-L-methionine-dependent methyltransferases superfamily protein
Locationchr8: 46356170 .. 46359707 (+)
RNA-Seq ExpressionLag0030320
SyntenyLag0030320
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGATCCGATTCCGTTGTCAATGGCGATTCCTTTCGAACTCCTCCCTTATTCAAGATCGTTACGTTCGTTCTTGTTTCTGTCACCTTCTTTTACTTTGGGAAGCATTGGTCCGATGGGTATCAGCAGCTTATCTTCTACCCGAGTACCAGTCAAGCTCCTTCGGTTTCACTTTCGCCCAACTATAATAAGCAATTCAATATCTCTAATCTGATTGATCACAATGACACGCAGACGCTTCCTGATAAGACTTTGAATCTCGACCCAACTCCGTCGCCGTTGCTTGGTGCTAATCCTCCTCCTCCTCCTCCGCCGTCCGATTCGGTGCAGAGATTTGGAATTGTGGACGAGAATGGAACAATGTCGGATCAGTTTGAGGTCGGGGATTTGGATCCTGAATATGTGGAGAACTGGGGGAATTCCACCGAGGTTGACAATGGCGAGGGCGGTGCTCGGAACTTCCGGATTAAGAAGTTTGGGCTCTGCCCTCAGAACATGAGTGAGTATATACCTTGTTTGGATAATGCAGAGGCAATTGCAAAGCTCAAGTCAACGGAGAAAGGGGAAAAGTTCGAGAGGCATTGCCCAGTAGCAGGTGGAGGTTTCGATTGCTTGATTCCGCCGCCGAAGGGGTATCAAGCCCCGATCCCGTGGCCGAAAAGCAGAGACGAGGTATTGCTAGCTTACAATAATTAGTAATTGCAGAAATTGAACGATTTGAAACAACTATAAATAACTAAAGAGTTGGTTCTTAGTTGAAAGTATTTTGGAGTTTTCTTAACTTAGGCATATGATTTGTTTTAGGTCTGGTTCAGCAATGTTCCTCATACTCGTCTAGTTGAGGATAAAGGAGGCCAAAACTGGATTACCAGAGACAAAGATAAGTTTAGGTTTCCTGGAGGTGGTACACAGTTTATACATGGTGCAGATGAATATTTGGATCATATTTCAAAGGTAAATAAGACTGATTCTGAAAGTTCAAAATTGAAATTAAGCATTCGGTGACATGGCATCTGATGCTTTTCTTCTCTTTATTTGACTAGATGATCCCTGATATTGCATTTGGTCGTCATACCCGCGTTGTCTTGGATATTGGATGTGGTGTTGCTAGTTTTGGCGCCTATTTGCTGTCACGGAATGTCGTTACAATGTCCATTGCTCCAAAAGATGTTCATGAGAATCAGATTCAGTTTGCTCTTGAGCGTGGTGTGCCTGCAATGGTTGCTGCATTTGCAACTCATCGCTTATTGTATCCCAGTCAAGCTTTTGACTTGATCCATTGTTCGCGATGCAGAATCAACTGGACTCGTGACGGTACTTTTTTCTATCTGATCCACCATTTATGTCACATAAACATGAGTAGGGTAATGAGTGGAATTCTCTTTGGTACTCAATTTAAGAAAGACATTTATCTTTTCTAGAACTATTCTAGACATTCATTAGCTCTGCTTCATATCAGATTCAATAAGCAAATGAATACTATATTATAAATTAATATTCTTTTTAGGGAACATAGAACTATAATTTAAACTCCCTAATATTTGTGAAGAGTGAAACTTATTGTTTTCTCCCCTCGGGCACATCATTGTTGTCAAAGAGAAGCATCATAATTGCTATTTTACTTAGTTGGTCTTCAAGACCTCAGTGAATTTCAATTGATTTCCTTGAAATTCATGTTTCTAATGTGTCAGAAATTGTCTAGTTAAATTATCTGCCTGGTGAAGTTTTGCTCTATGAAGCCATTTACTTTTGATTTTTCTTATGGCTGTTGATTCTTCATCTTCAACTTGTCATATGAAGATGGCATTTTGCTGCTTGAGGTTAACCGAATGCTCAGGGCTGGAGGATACTTTGCGTGGGCAGCTCAACCTGTTTATAAGCATGAAGAAGTTTTGGAGGAACAGTGGGAAGGTAACTAAAGTTTTCTTAAGTTTTCCTTTGAAGTTTGGTCTGGACGGGCAGTTAGAACTTTGGAATTTTTGCACTCCACAGTCATGCGCAGACCATTTGCTATCTTACTAACACAAACACTCTTCTCTTGCAAGTTCTTTTTGTCCTCTTCTAATAAGTAACAGAACATGTTTGATGCAAGTTTTAAATGATAAGTACAAACATCTAAGATTATTGTGGCAACTTGTGTGGTGTTTGTGCCTACTTTTATTAGTTTGGGTGAAATTGACCGGATCTCATCTCTGAACTTTCAAGTGTGGTTTATATGTGTGTTCTTTTTACTTTTTGGTTGCCAGAGATGCTCAATTTGACCACCCGACTTTGTTGGGGTTTCGTGAAGAAAGATGGATATATAGCAATTTGGCAGAAGCCATTAAATAATAGTTGTTATCTAAATCGTGAGGCAGGAACTAAACCCCCTCTCTGTGATCAAAATGACGACCCAGACAGAGTATGGTATGGATGTTAAGGTTATTAGAGAATTGTAGTGTGTGTATTTCCGTGTTTTTCTTTTCTTTTAGAGTTAGTTTCATTGATTACTTCTTTGGAGACAGGAATGCAGATCTTAAGCCATGCATCTCTCGTATTCCAGAAGATGGTTACGGGGCAAATATCACTGTTTGGCCTGAGCGGTTGCATACACCACCCGGTAGGCTTCAGACAATTCAATACGATGCCTACATTTCCAGAAATGAGCTCTTCAAGGCAGAATCTAAATACTGGAATGAGATAATAGACAGCTATGTTCGCGCTTTTCACTGGAAGACGTTTAGACTACGAAACGTAATGGATATGAAAGCTGGATTTGGAGGGTATGGATATCAACCACTCTTCCACTGAACTGCCATGTCTTCATTCTTTTCCTTACATATGGTTAAGATCTTAAGTGTTAACAGATTTGCAGCGGCATTGATCGATCTTAAACTAGATTGCTGGGTGTTGAATGTTGTTCCTGTCAGTGGGGCCAACACCTTGCCTGTTATTTATGATCGTGGACTTATTGGAGTTATGCACGATTGGTAACATACGAAACTTTTCCTAAATTGATTAGCATCAAGCTTTTGAATGCATGCCTTATGCTACAGTGATATAGAAGTAATGATGATAATTAATAACAGAGCAAATAATATTGTAACATGTCAGGTGTGAACCATTTGATACATATCCAAGAACGTATGATTTATTGCACGCAGCTGGTTTGTTCTCAGTCGAAAGGAAAAGGTATCCCGAATTTAGTTGATTTCTCTGTTTTTCTTTTAGATTACTTTATTCCTAGTTTTCTTCTTCTTATTTGTAATGATGTATTCCAGTGAGCTCAATGGCATGAATTTGAAATCATTGTGATGGGTACCAACTGTTCAATTAATGTGTGTGTTTCAGGTGCAATATGTCAACCATAATGCTTGAGATGGATAGAATACTGAGACCGGGTGGACGAGTGTACATCCGAGACTCCGTTGCAGTCATGGATGAGCTTCAAGACATTGCAAAGGCCATGGGTTGGCGCGCGAATGTGCGGGATACGTCAGAAGGTCCTCATGCAAGCTATAAGATCATGATGGCAGATAAGCTCCTCTTAAATGCATAA

mRNA sequence

ATGAGATCCGATTCCGTTGTCAATGGCGATTCCTTTCGAACTCCTCCCTTATTCAAGATCGTTACGTTCGTTCTTGTTTCTGTCACCTTCTTTTACTTTGGGAAGCATTGGTCCGATGGGTATCAGCAGCTTATCTTCTACCCGAGTACCAGTCAAGCTCCTTCGGTTTCACTTTCGCCCAACTATAATAAGCAATTCAATATCTCTAATCTGATTGATCACAATGACACGCAGACGCTTCCTGATAAGACTTTGAATCTCGACCCAACTCCGTCGCCGTTGCTTGGTGCTAATCCTCCTCCTCCTCCTCCGCCGTCCGATTCGGTGCAGAGATTTGGAATTGTGGACGAGAATGGAACAATGTCGGATCAGTTTGAGGTCGGGGATTTGGATCCTGAATATGTGGAGAACTGGGGGAATTCCACCGAGGTTGACAATGGCGAGGGCGGTGCTCGGAACTTCCGGATTAAGAAGTTTGGGCTCTGCCCTCAGAACATGAGTGAGTATATACCTTGTTTGGATAATGCAGAGGCAATTGCAAAGCTCAAGTCAACGGAGAAAGGGGAAAAGTTCGAGAGGCATTGCCCAGTAGCAGGTGGAGGTTTCGATTGCTTGATTCCGCCGCCGAAGGGGTATCAAGCCCCGATCCCGTGGCCGAAAAGCAGAGACGAGGTCTGGTTCAGCAATGTTCCTCATACTCGTCTAGTTGAGGATAAAGGAGGCCAAAACTGGATTACCAGAGACAAAGATAAGTTTAGGTTTCCTGGAGGTGGTACACAGTTTATACATGGTGCAGATGAATATTTGGATCATATTTCAAAGATGATCCCTGATATTGCATTTGGTCGTCATACCCGCGTTGTCTTGGATATTGGATGTGGTGTTGCTAGTTTTGGCGCCTATTTGCTGTCACGGAATGTCGTTACAATGTCCATTGCTCCAAAAGATGTTCATGAGAATCAGATTCAGTTTGCTCTTGAGCGTGGTGTGCCTGCAATGGTTGCTGCATTTGCAACTCATCGCTTATTGTATCCCAGTCAAGCTTTTGACTTGATCCATTGTTCGCGATGCAGAATCAACTGGACTCGTGACGATGGCATTTTGCTGCTTGAGGTTAACCGAATGCTCAGGGCTGGAGGATACTTTGCGTGGGCAGCTCAACCTGTTTATAAGCATGAAGAAGTTTTGGAGGAACAGTGGGAAGAGATGCTCAATTTGACCACCCGACTTTGTTGGGGTTTCGTGAAGAAAGATGGATATATAGCAATTTGGCAGAAGCCATTAAATAATAGTTGTTATCTAAATCGTGAGGCAGGAACTAAACCCCCTCTCTGTGATCAAAATGACGACCCAGACAGAGTATGGAATGCAGATCTTAAGCCATGCATCTCTCGTATTCCAGAAGATGGTTACGGGGCAAATATCACTGTTTGGCCTGAGCGGTTGCATACACCACCCGGTAGGCTTCAGACAATTCAATACGATGCCTACATTTCCAGAAATGAGCTCTTCAAGGCAGAATCTAAATACTGGAATGAGATAATAGACAGCTATGTTCGCGCTTTTCACTGGAAGACGTTTAGACTACGAAACGTAATGGATATGAAAGCTGGATTTGGAGGATTTGCAGCGGCATTGATCGATCTTAAACTAGATTGCTGGGTGTTGAATGTTGTTCCTGTCAGTGGGGCCAACACCTTGCCTGTTATTTATGATCGTGGACTTATTGGAGTTATGCACGATTGGTGTGAACCATTTGATACATATCCAAGAACGTATGATTTATTGCACGCAGCTGGTTTGTTCTCAGTCGAAAGGAAAAGGTGCAATATGTCAACCATAATGCTTGAGATGGATAGAATACTGAGACCGGGTGGACGAGTGTACATCCGAGACTCCGTTGCAGTCATGGATGAGCTTCAAGACATTGCAAAGGCCATGGGTTGGCGCGCGAATGTGCGGGATACGTCAGAAGGTCCTCATGCAAGCTATAAGATCATGATGGCAGATAAGCTCCTCTTAAATGCATAA

Coding sequence (CDS)

ATGAGATCCGATTCCGTTGTCAATGGCGATTCCTTTCGAACTCCTCCCTTATTCAAGATCGTTACGTTCGTTCTTGTTTCTGTCACCTTCTTTTACTTTGGGAAGCATTGGTCCGATGGGTATCAGCAGCTTATCTTCTACCCGAGTACCAGTCAAGCTCCTTCGGTTTCACTTTCGCCCAACTATAATAAGCAATTCAATATCTCTAATCTGATTGATCACAATGACACGCAGACGCTTCCTGATAAGACTTTGAATCTCGACCCAACTCCGTCGCCGTTGCTTGGTGCTAATCCTCCTCCTCCTCCTCCGCCGTCCGATTCGGTGCAGAGATTTGGAATTGTGGACGAGAATGGAACAATGTCGGATCAGTTTGAGGTCGGGGATTTGGATCCTGAATATGTGGAGAACTGGGGGAATTCCACCGAGGTTGACAATGGCGAGGGCGGTGCTCGGAACTTCCGGATTAAGAAGTTTGGGCTCTGCCCTCAGAACATGAGTGAGTATATACCTTGTTTGGATAATGCAGAGGCAATTGCAAAGCTCAAGTCAACGGAGAAAGGGGAAAAGTTCGAGAGGCATTGCCCAGTAGCAGGTGGAGGTTTCGATTGCTTGATTCCGCCGCCGAAGGGGTATCAAGCCCCGATCCCGTGGCCGAAAAGCAGAGACGAGGTCTGGTTCAGCAATGTTCCTCATACTCGTCTAGTTGAGGATAAAGGAGGCCAAAACTGGATTACCAGAGACAAAGATAAGTTTAGGTTTCCTGGAGGTGGTACACAGTTTATACATGGTGCAGATGAATATTTGGATCATATTTCAAAGATGATCCCTGATATTGCATTTGGTCGTCATACCCGCGTTGTCTTGGATATTGGATGTGGTGTTGCTAGTTTTGGCGCCTATTTGCTGTCACGGAATGTCGTTACAATGTCCATTGCTCCAAAAGATGTTCATGAGAATCAGATTCAGTTTGCTCTTGAGCGTGGTGTGCCTGCAATGGTTGCTGCATTTGCAACTCATCGCTTATTGTATCCCAGTCAAGCTTTTGACTTGATCCATTGTTCGCGATGCAGAATCAACTGGACTCGTGACGATGGCATTTTGCTGCTTGAGGTTAACCGAATGCTCAGGGCTGGAGGATACTTTGCGTGGGCAGCTCAACCTGTTTATAAGCATGAAGAAGTTTTGGAGGAACAGTGGGAAGAGATGCTCAATTTGACCACCCGACTTTGTTGGGGTTTCGTGAAGAAAGATGGATATATAGCAATTTGGCAGAAGCCATTAAATAATAGTTGTTATCTAAATCGTGAGGCAGGAACTAAACCCCCTCTCTGTGATCAAAATGACGACCCAGACAGAGTATGGAATGCAGATCTTAAGCCATGCATCTCTCGTATTCCAGAAGATGGTTACGGGGCAAATATCACTGTTTGGCCTGAGCGGTTGCATACACCACCCGGTAGGCTTCAGACAATTCAATACGATGCCTACATTTCCAGAAATGAGCTCTTCAAGGCAGAATCTAAATACTGGAATGAGATAATAGACAGCTATGTTCGCGCTTTTCACTGGAAGACGTTTAGACTACGAAACGTAATGGATATGAAAGCTGGATTTGGAGGATTTGCAGCGGCATTGATCGATCTTAAACTAGATTGCTGGGTGTTGAATGTTGTTCCTGTCAGTGGGGCCAACACCTTGCCTGTTATTTATGATCGTGGACTTATTGGAGTTATGCACGATTGGTGTGAACCATTTGATACATATCCAAGAACGTATGATTTATTGCACGCAGCTGGTTTGTTCTCAGTCGAAAGGAAAAGGTGCAATATGTCAACCATAATGCTTGAGATGGATAGAATACTGAGACCGGGTGGACGAGTGTACATCCGAGACTCCGTTGCAGTCATGGATGAGCTTCAAGACATTGCAAAGGCCATGGGTTGGCGCGCGAATGTGCGGGATACGTCAGAAGGTCCTCATGCAAGCTATAAGATCATGATGGCAGATAAGCTCCTCTTAAATGCATAA

Protein sequence

MRSDSVVNGDSFRTPPLFKIVTFVLVSVTFFYFGKHWSDGYQQLIFYPSTSQAPSVSLSPNYNKQFNISNLIDHNDTQTLPDKTLNLDPTPSPLLGANPPPPPPPSDSVQRFGIVDENGTMSDQFEVGDLDPEYVENWGNSTEVDNGEGGARNFRIKKFGLCPQNMSEYIPCLDNAEAIAKLKSTEKGEKFERHCPVAGGGFDCLIPPPKGYQAPIPWPKSRDEVWFSNVPHTRLVEDKGGQNWITRDKDKFRFPGGGTQFIHGADEYLDHISKMIPDIAFGRHTRVVLDIGCGVASFGAYLLSRNVVTMSIAPKDVHENQIQFALERGVPAMVAAFATHRLLYPSQAFDLIHCSRCRINWTRDDGILLLEVNRMLRAGGYFAWAAQPVYKHEEVLEEQWEEMLNLTTRLCWGFVKKDGYIAIWQKPLNNSCYLNREAGTKPPLCDQNDDPDRVWNADLKPCISRIPEDGYGANITVWPERLHTPPGRLQTIQYDAYISRNELFKAESKYWNEIIDSYVRAFHWKTFRLRNVMDMKAGFGGFAAALIDLKLDCWVLNVVPVSGANTLPVIYDRGLIGVMHDWCEPFDTYPRTYDLLHAAGLFSVERKRCNMSTIMLEMDRILRPGGRVYIRDSVAVMDELQDIAKAMGWRANVRDTSEGPHASYKIMMADKLLLNA
Homology
BLAST of Lag0030320 vs. NCBI nr
Match: XP_038885726.1 (probable methyltransferase PMT11 [Benincasa hispida])

HSP 1 Score: 1340.5 bits (3468), Expect = 0.0e+00
Identity = 628/677 (92.76%), Postives = 647/677 (95.57%), Query Frame = 0

Query: 1   MRSDSVVNGDSFRTPPLFKIVTFVLVSVTFFYFGKHWSDGYQQLIFY-PSTSQAPSVSLS 60
           MRSDS+ NGDSFR PPLFKI TFV V+VTFFYFGKHWSDGYQQLIF+  +T++ PSVSLS
Sbjct: 1   MRSDSLGNGDSFRAPPLFKIATFVFVAVTFFYFGKHWSDGYQQLIFFTTTTTKPPSVSLS 60

Query: 61  PNYNKQFNISNLIDHNDTQTLPDKTLNLDPTPSPLLGANPPPPPPPSDSVQRFGIVDENG 120
           PNYNK FNISNLIDHNDTQTLPDKTLNLDPTPSPLL  N  PPPPPSDSVQ+FGIVDENG
Sbjct: 61  PNYNKHFNISNLIDHNDTQTLPDKTLNLDPTPSPLLATN--PPPPPSDSVQKFGIVDENG 120

Query: 121 TMSDQFEVGDLDPEYVENWGNSTEVDNGEGGARNFRIKKFGLCPQNMSEYIPCLDNAEAI 180
           TMSDQFEVGD DPEYVENWGNST+VDNGEGG+R FRIKKFGLCPQNMSEYIPCLDNA+AI
Sbjct: 121 TMSDQFEVGDFDPEYVENWGNSTQVDNGEGGSRRFRIKKFGLCPQNMSEYIPCLDNADAI 180

Query: 181 AKLKSTEKGEKFERHCPVAGGGFDCLIPPPKGYQAPIPWPKSRDEVWFSNVPHTRLVEDK 240
           AKL+STE+GEKFERHCPVAGGGFDCLIPPPKGYQ PIPWP+SRDEVWFSNVPHTRLVEDK
Sbjct: 181 AKLESTERGEKFERHCPVAGGGFDCLIPPPKGYQTPIPWPRSRDEVWFSNVPHTRLVEDK 240

Query: 241 GGQNWITRDKDKFRFPGGGTQFIHGADEYLDHISKMIPDIAFGRHTRVVLDIGCGVASFG 300
           GGQNWITRDKDKFRFPGGGTQFIHGADEYLDHISKMIPDIAFGRHTRVVLDIGCGVASFG
Sbjct: 241 GGQNWITRDKDKFRFPGGGTQFIHGADEYLDHISKMIPDIAFGRHTRVVLDIGCGVASFG 300

Query: 301 AYLLSRNVVTMSIAPKDVHENQIQFALERGVPAMVAAFATHRLLYPSQAFDLIHCSRCRI 360
           AYLLSRNV TMSIAPKDVHENQIQFALERGVPAMVAAFATHRLLYPSQAFDLIHCSRCRI
Sbjct: 301 AYLLSRNVTTMSIAPKDVHENQIQFALERGVPAMVAAFATHRLLYPSQAFDLIHCSRCRI 360

Query: 361 NWTRDDGILLLEVNRMLRAGGYFAWAAQPVYKHEEVLEEQWEEMLNLTTRLCWGFVKKDG 420
           NWTRDDGILLLEVNRMLRAGGYFAWAAQPVYKHEEVLEEQWEEMLNLT RLCW FVKKDG
Sbjct: 361 NWTRDDGILLLEVNRMLRAGGYFAWAAQPVYKHEEVLEEQWEEMLNLTNRLCWEFVKKDG 420

Query: 421 YIAIWQKPLNNSCYLNREAGTKPPLCDQNDDPDRVWNADLKPCISRIPEDGYGANITVWP 480
           YIAIWQKPLNNSCYLNREA TKPPLCDQNDDPDRVWN +LK CISR+PEDGYG NITVWP
Sbjct: 421 YIAIWQKPLNNSCYLNREAATKPPLCDQNDDPDRVWNVNLKTCISRLPEDGYGGNITVWP 480

Query: 481 ERLHTPPGRLQTIQYDAYISRNELFKAESKYWNEIIDSYVRAFHWKTFRLRNVMDMKAGF 540
            RLHTPP RLQTIQYDAYISRNELF+AESKYWNEIIDSYVRAFHWK+FRLRNVMDMKAGF
Sbjct: 481 ARLHTPPDRLQTIQYDAYISRNELFQAESKYWNEIIDSYVRAFHWKSFRLRNVMDMKAGF 540

Query: 541 GGFAAALIDLKLDCWVLNVVPVSGANTLPVIYDRGLIGVMHDWCEPFDTYPRTYDLLHAA 600
           GGFAAALIDLKLDCWVLNVVPVSG NTLPVIYDRGLIGVMHDWCEPFDTYPRTYDLLHAA
Sbjct: 541 GGFAAALIDLKLDCWVLNVVPVSGPNTLPVIYDRGLIGVMHDWCEPFDTYPRTYDLLHAA 600

Query: 601 GLFSVERKRCNMSTIMLEMDRILRPGGRVYIRDSVAVMDELQDIAKAMGWRANVRDTSEG 660
           GLFSVER RCNMSTIMLEMDRILRPGGRVYIRDSVAVMDELQDI KAMGW  N+RDTSEG
Sbjct: 601 GLFSVERNRCNMSTIMLEMDRILRPGGRVYIRDSVAVMDELQDIGKAMGWHVNLRDTSEG 660

Query: 661 PHASYKIMMADKLLLNA 677
           PHASYKIMMADK+LL A
Sbjct: 661 PHASYKIMMADKVLLRA 675

BLAST of Lag0030320 vs. NCBI nr
Match: XP_023551343.1 (probable methyltransferase PMT11 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1327.0 bits (3433), Expect = 0.0e+00
Identity = 622/676 (92.01%), Postives = 643/676 (95.12%), Query Frame = 0

Query: 1   MRSDSVVNGDSFRTPPLFKIVTFVLVSVTFFYFGKHWSDGYQQLIFYPSTSQAPSVSLSP 60
           MRSDS+VNGDSFR PPLFKI T V VSVTFFYFGKHWSDGYQQLIF+ STSQAP+VSLSP
Sbjct: 1   MRSDSLVNGDSFRAPPLFKIATLVFVSVTFFYFGKHWSDGYQQLIFFQSTSQAPTVSLSP 60

Query: 61  NYNKQFNISNLIDHNDTQTLPDKTLNLDPTPSPLLGANPPPPPPPSDSVQRFGIVDENGT 120
           NYNK FN S LI  NDT+TLPDKTLNLDPTPSPLL    P PPPPSDSVQRFGIVDENGT
Sbjct: 61  NYNKHFNTSTLIQQNDTETLPDKTLNLDPTPSPLLA---PNPPPPSDSVQRFGIVDENGT 120

Query: 121 MSDQFEVGDLDPEYVENWGNSTEVDNGEGGARNFRIKKFGLCPQNMSEYIPCLDNAEAIA 180
           MSDQFEVGDLDPEYVEN GNSTEVDNGEGG+R+FRIKKF LCPQNMSEYIPCLDN +AI+
Sbjct: 121 MSDQFEVGDLDPEYVENRGNSTEVDNGEGGSRSFRIKKFALCPQNMSEYIPCLDNVDAIS 180

Query: 181 KLKSTEKGEKFERHCPVAGGGFDCLIPPPKGYQAPIPWPKSRDEVWFSNVPHTRLVEDKG 240
           KLKS EKGEKFERHCPVAGGGF+CLIPPPKGYQ PI WP+SRD+VWFSNVPHTRLVEDKG
Sbjct: 181 KLKSVEKGEKFERHCPVAGGGFNCLIPPPKGYQTPIHWPRSRDQVWFSNVPHTRLVEDKG 240

Query: 241 GQNWITRDKDKFRFPGGGTQFIHGADEYLDHISKMIPDIAFGRHTRVVLDIGCGVASFGA 300
           GQNWITRDKDKF+FPGGGTQFIHGADEYLDHISKMIPDIAFG HTRVVLDIGCGVASFGA
Sbjct: 241 GQNWITRDKDKFKFPGGGTQFIHGADEYLDHISKMIPDIAFGHHTRVVLDIGCGVASFGA 300

Query: 301 YLLSRNVVTMSIAPKDVHENQIQFALERGVPAMVAAFATHRLLYPSQAFDLIHCSRCRIN 360
           YLLSRNV+TMSIAPKDVHENQIQFALERGVPAMVAAFATHRLLYPSQAFDLIHCSRCRIN
Sbjct: 301 YLLSRNVITMSIAPKDVHENQIQFALERGVPAMVAAFATHRLLYPSQAFDLIHCSRCRIN 360

Query: 361 WTRDDGILLLEVNRMLRAGGYFAWAAQPVYKHEEVLEEQWEEMLNLTTRLCWGFVKKDGY 420
           WTRDDGILLLE+NRMLRAGGYFAWAAQPVYKHEEVLEEQWEEMLNLTTRLCW FVKKDGY
Sbjct: 361 WTRDDGILLLELNRMLRAGGYFAWAAQPVYKHEEVLEEQWEEMLNLTTRLCWEFVKKDGY 420

Query: 421 IAIWQKPLNNSCYLNREAGTKPPLCDQNDDPDRVWNADLKPCISRIPEDGYGANITVWPE 480
           IAIWQKPLNNSCYLNR+A TKPPLCDQ+DDPDRVWN DLK CISR+PEDGYGAN+TVWPE
Sbjct: 421 IAIWQKPLNNSCYLNRQAETKPPLCDQDDDPDRVWNVDLKTCISRLPEDGYGANVTVWPE 480

Query: 481 RLHTPPGRLQTIQYDAYISRNELFKAESKYWNEIIDSYVRAFHWKTFRLRNVMDMKAGFG 540
           RLHTPPGRLQTIQYDAYISRNELFKAESKYWNEIIDSYVRAFHWKTFRLRNVMDM+AGFG
Sbjct: 481 RLHTPPGRLQTIQYDAYISRNELFKAESKYWNEIIDSYVRAFHWKTFRLRNVMDMRAGFG 540

Query: 541 GFAAALIDLKLDCWVLNVVPVSGANTLPVIYDRGLIGVMHDWCEPFDTYPRTYDLLHAAG 600
           GFAAAL+DLKLDCWVLNVVPVSG NTLPVIYDRGLIGVMHDWCEPFDTYPRTYDLLHAAG
Sbjct: 541 GFAAALVDLKLDCWVLNVVPVSGPNTLPVIYDRGLIGVMHDWCEPFDTYPRTYDLLHAAG 600

Query: 601 LFSVERKRCNMSTIMLEMDRILRPGGRVYIRDSVAVMDELQDIAKAMGWRANVRDTSEGP 660
           LFSVERKRCN+STIMLEMDRILRPGGRVYIRDSVAVMDELQ I KAMGW  NVRDTSEGP
Sbjct: 601 LFSVERKRCNISTIMLEMDRILRPGGRVYIRDSVAVMDELQGIGKAMGWHVNVRDTSEGP 660

Query: 661 HASYKIMMADKLLLNA 677
           HASYKIMMADKLL  A
Sbjct: 661 HASYKIMMADKLLTRA 673

BLAST of Lag0030320 vs. NCBI nr
Match: XP_023552424.1 (probable methyltransferase PMT11 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1325.1 bits (3428), Expect = 0.0e+00
Identity = 622/676 (92.01%), Postives = 644/676 (95.27%), Query Frame = 0

Query: 1   MRSDSVVNGDSFRTPPLFKIVTFVLVSVTFFYFGKHWSDGYQQLIFYPSTSQAPSVSLSP 60
           MRSDS+VN +SFR  PLFKI  F+L SVTFFYFGKHWSDGYQQL+FY  T QA SVSLSP
Sbjct: 1   MRSDSLVNAESFRASPLFKIAIFLLASVTFFYFGKHWSDGYQQLVFYTGTLQASSVSLSP 60

Query: 61  NYNKQFNISNLIDHNDTQTLPDKTLNLDPTPSPLLGANPPPPPPPSDSVQRFGIVDENGT 120
           NYNK FNISNLI  NDT  LPDKTLNLDPTP PLL  +  PPPPPSDSVQRFGI+DENGT
Sbjct: 61  NYNKHFNISNLIHQNDTHVLPDKTLNLDPTPPPLLRTD--PPPPPSDSVQRFGILDENGT 120

Query: 121 MSDQFEVGDLDPEYVENWGNSTEVDNGEGGARNFRIKKFGLCPQNMSEYIPCLDNAEAIA 180
           MSDQFEVGDLDPEYVENWGNSTEVD+GEG +RNFRIKKFGLCPQNMSEYIPCLDN EAIA
Sbjct: 121 MSDQFEVGDLDPEYVENWGNSTEVDHGEGSSRNFRIKKFGLCPQNMSEYIPCLDNQEAIA 180

Query: 181 KLKSTEKGEKFERHCPVAGGGFDCLIPPPKGYQAPIPWPKSRDEVWFSNVPHTRLVEDKG 240
           KLKST+KGEKFERHCPVAGGGFDCLIPPPKGY+APIPWPKSRDEVWFSNVPHTRLVEDKG
Sbjct: 181 KLKSTDKGEKFERHCPVAGGGFDCLIPPPKGYRAPIPWPKSRDEVWFSNVPHTRLVEDKG 240

Query: 241 GQNWITRDKDKFRFPGGGTQFIHGADEYLDHISKMIPDIAFGRHTRVVLDIGCGVASFGA 300
           GQNWITRDKDKFRFPGGGTQFIHGADEYLDHISKMIPDIAFG HTRVV+DIGCGVASFGA
Sbjct: 241 GQNWITRDKDKFRFPGGGTQFIHGADEYLDHISKMIPDIAFGHHTRVVMDIGCGVASFGA 300

Query: 301 YLLSRNVVTMSIAPKDVHENQIQFALERGVPAMVAAFATHRLLYPSQAFDLIHCSRCRIN 360
           YLLSR+VVTMSIAPKDVHENQIQFALERGVPAMVAAFATHRLLYPSQAFDLIHCSRCRIN
Sbjct: 301 YLLSRDVVTMSIAPKDVHENQIQFALERGVPAMVAAFATHRLLYPSQAFDLIHCSRCRIN 360

Query: 361 WTRDDGILLLEVNRMLRAGGYFAWAAQPVYKHEEVLEEQWEEMLNLTTRLCWGFVKKDGY 420
           WTRDDGILLLEVNRMLRAGGYFAWAAQPVYKHEEVLEEQW EMLNLTTRLCW FVKKDGY
Sbjct: 361 WTRDDGILLLEVNRMLRAGGYFAWAAQPVYKHEEVLEEQWGEMLNLTTRLCWEFVKKDGY 420

Query: 421 IAIWQKPLNNSCYLNREAGTKPPLCDQNDDPDRVWNADLKPCISRIPEDGYGANITVWPE 480
           IAIW+KPLNN+CYLNREAGTKPPLCDQNDDPDRVWN DLK CISR+PEDGYGANITVWP 
Sbjct: 421 IAIWRKPLNNTCYLNREAGTKPPLCDQNDDPDRVWNEDLKTCISRLPEDGYGANITVWPA 480

Query: 481 RLHTPPGRLQTIQYDAYISRNELFKAESKYWNEIIDSYVRAFHWKTFRLRNVMDMKAGFG 540
           RLHTPPGRLQTIQ+DAYISRNELFKAESKYWNEII+SYVRAFHWKTFRLRNVMDMKAGFG
Sbjct: 481 RLHTPPGRLQTIQHDAYISRNELFKAESKYWNEIIESYVRAFHWKTFRLRNVMDMKAGFG 540

Query: 541 GFAAALIDLKLDCWVLNVVPVSGANTLPVIYDRGLIGVMHDWCEPFDTYPRTYDLLHAAG 600
           GFAAALIDLKLDCWVLNVVPVSG NTLPVIYDRGL+GVMHDWCEPFDTYPRTYDLLHAAG
Sbjct: 541 GFAAALIDLKLDCWVLNVVPVSGFNTLPVIYDRGLLGVMHDWCEPFDTYPRTYDLLHAAG 600

Query: 601 LFSVERKRCNMSTIMLEMDRILRPGGRVYIRDSVAVMDELQDIAKAMGWRANVRDTSEGP 660
           LFSVERKRCNMSTIMLEMDRILRPGGRVYIRDSVAV+DELQ IAKAMGWR +V DTSEGP
Sbjct: 601 LFSVERKRCNMSTIMLEMDRILRPGGRVYIRDSVAVIDELQHIAKAMGWRVDVHDTSEGP 660

Query: 661 HASYKIMMADKLLLNA 677
           HASYKIMMA+KLLL+A
Sbjct: 661 HASYKIMMANKLLLSA 674

BLAST of Lag0030320 vs. NCBI nr
Match: KAG6578419.1 (putative methyltransferase PMT11, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1325.1 bits (3428), Expect = 0.0e+00
Identity = 620/676 (91.72%), Postives = 643/676 (95.12%), Query Frame = 0

Query: 1   MRSDSVVNGDSFRTPPLFKIVTFVLVSVTFFYFGKHWSDGYQQLIFYPSTSQAPSVSLSP 60
           MRSDS+VNGDSFR PPLFKI T V VSVTFFYFGKHWSDGYQQLIF+ STSQAP+VSLSP
Sbjct: 44  MRSDSLVNGDSFRAPPLFKIATLVFVSVTFFYFGKHWSDGYQQLIFFQSTSQAPTVSLSP 103

Query: 61  NYNKQFNISNLIDHNDTQTLPDKTLNLDPTPSPLLGANPPPPPPPSDSVQRFGIVDENGT 120
           NYNK FNIS LI  ND +TLPDKTLNLDPTPSPLL    P PPPPSDSVQRFGIVDENGT
Sbjct: 104 NYNKHFNISTLIQQNDAETLPDKTLNLDPTPSPLLA---PNPPPPSDSVQRFGIVDENGT 163

Query: 121 MSDQFEVGDLDPEYVENWGNSTEVDNGEGGARNFRIKKFGLCPQNMSEYIPCLDNAEAIA 180
           MSDQFEVGDLDPEYVEN GNSTEVDNG+GG+R+FRIKKF LCPQNMSEYIPCLDN +AI+
Sbjct: 164 MSDQFEVGDLDPEYVENRGNSTEVDNGKGGSRSFRIKKFALCPQNMSEYIPCLDNVDAIS 223

Query: 181 KLKSTEKGEKFERHCPVAGGGFDCLIPPPKGYQAPIPWPKSRDEVWFSNVPHTRLVEDKG 240
           KLKS EKGEKFERHCPV GGGF+CLIPPPKGYQ PIPWP+SRD+VWFSNVPHTRLVEDKG
Sbjct: 224 KLKSVEKGEKFERHCPVTGGGFNCLIPPPKGYQTPIPWPRSRDQVWFSNVPHTRLVEDKG 283

Query: 241 GQNWITRDKDKFRFPGGGTQFIHGADEYLDHISKMIPDIAFGRHTRVVLDIGCGVASFGA 300
           GQNWITRDKDKF+FPGGGTQFIHGADEYLDHISKMIPDIAFG HTRVVLDIGCGVASFGA
Sbjct: 284 GQNWITRDKDKFKFPGGGTQFIHGADEYLDHISKMIPDIAFGHHTRVVLDIGCGVASFGA 343

Query: 301 YLLSRNVVTMSIAPKDVHENQIQFALERGVPAMVAAFATHRLLYPSQAFDLIHCSRCRIN 360
           YLLSRNV+TMSIAPKDVHENQIQFALERGVPAMVAAFATHRLLYPSQAFDLIHCSRCRIN
Sbjct: 344 YLLSRNVITMSIAPKDVHENQIQFALERGVPAMVAAFATHRLLYPSQAFDLIHCSRCRIN 403

Query: 361 WTRDDGILLLEVNRMLRAGGYFAWAAQPVYKHEEVLEEQWEEMLNLTTRLCWGFVKKDGY 420
           WTRDDGILLLE+NRMLRAGGYFAWAAQPVYKHEEVLEEQWEEMLNLTTRLCW FVKKDGY
Sbjct: 404 WTRDDGILLLELNRMLRAGGYFAWAAQPVYKHEEVLEEQWEEMLNLTTRLCWEFVKKDGY 463

Query: 421 IAIWQKPLNNSCYLNREAGTKPPLCDQNDDPDRVWNADLKPCISRIPEDGYGANITVWPE 480
           IAIW+KPLNNSCYLNR+A TKPPLCDQ+DDPDRVWN DLK CISR+PEDGYGAN+TVWPE
Sbjct: 464 IAIWRKPLNNSCYLNRQAETKPPLCDQDDDPDRVWNVDLKTCISRLPEDGYGANVTVWPE 523

Query: 481 RLHTPPGRLQTIQYDAYISRNELFKAESKYWNEIIDSYVRAFHWKTFRLRNVMDMKAGFG 540
           RLHTPPGRLQTIQYDAYISRNELFKAESKYWNEIIDSYVRAFHWKTFRLRNVMDM+AGFG
Sbjct: 524 RLHTPPGRLQTIQYDAYISRNELFKAESKYWNEIIDSYVRAFHWKTFRLRNVMDMRAGFG 583

Query: 541 GFAAALIDLKLDCWVLNVVPVSGANTLPVIYDRGLIGVMHDWCEPFDTYPRTYDLLHAAG 600
           GFAAALIDLKLDCWVLNVVPVSG NTLPVIYDRGLIGVMHDWCEPFDTYPRTYDLLH+AG
Sbjct: 584 GFAAALIDLKLDCWVLNVVPVSGPNTLPVIYDRGLIGVMHDWCEPFDTYPRTYDLLHSAG 643

Query: 601 LFSVERKRCNMSTIMLEMDRILRPGGRVYIRDSVAVMDELQDIAKAMGWRANVRDTSEGP 660
           LFSVERKRCN+STIMLEMDRILRPGGRVYIRDSVAVMDELQ I KAMGW  NVRDTSEGP
Sbjct: 644 LFSVERKRCNISTIMLEMDRILRPGGRVYIRDSVAVMDELQGIGKAMGWHVNVRDTSEGP 703

Query: 661 HASYKIMMADKLLLNA 677
           HASYKIMMADKLL  A
Sbjct: 704 HASYKIMMADKLLTRA 716

BLAST of Lag0030320 vs. NCBI nr
Match: XP_022939686.1 (probable methyltransferase PMT11 [Cucurbita moschata] >KAG7015983.1 putative methyltransferase PMT11 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1325.1 bits (3428), Expect = 0.0e+00
Identity = 620/676 (91.72%), Postives = 644/676 (95.27%), Query Frame = 0

Query: 1   MRSDSVVNGDSFRTPPLFKIVTFVLVSVTFFYFGKHWSDGYQQLIFYPSTSQAPSVSLSP 60
           MRSDS+VNGDSFR PPLFKI T V VSVTFFYFGKHWSDGYQQLIF+ STSQAP+VSLSP
Sbjct: 1   MRSDSLVNGDSFRAPPLFKIATLVFVSVTFFYFGKHWSDGYQQLIFFQSTSQAPTVSLSP 60

Query: 61  NYNKQFNISNLIDHNDTQTLPDKTLNLDPTPSPLLGANPPPPPPPSDSVQRFGIVDENGT 120
           NY+K FNIS LI  NDT+TLPDKTLNLDPTPSPLL    P PPPPSDSVQRFGIVDENGT
Sbjct: 61  NYDKHFNISTLIQQNDTETLPDKTLNLDPTPSPLLA---PNPPPPSDSVQRFGIVDENGT 120

Query: 121 MSDQFEVGDLDPEYVENWGNSTEVDNGEGGARNFRIKKFGLCPQNMSEYIPCLDNAEAIA 180
           MSDQFEVGDLDPEYVEN GNSTEVDNG+GG+R+FRIKKF LCPQNMSEYIPCLDN +AI+
Sbjct: 121 MSDQFEVGDLDPEYVENRGNSTEVDNGKGGSRSFRIKKFALCPQNMSEYIPCLDNVDAIS 180

Query: 181 KLKSTEKGEKFERHCPVAGGGFDCLIPPPKGYQAPIPWPKSRDEVWFSNVPHTRLVEDKG 240
           KLKS EKGEKFERHCPV GGGF+CLIPPPKGYQ PIPWP+SRD+VWFSNVPHTRLVEDKG
Sbjct: 181 KLKSVEKGEKFERHCPVTGGGFNCLIPPPKGYQTPIPWPRSRDQVWFSNVPHTRLVEDKG 240

Query: 241 GQNWITRDKDKFRFPGGGTQFIHGADEYLDHISKMIPDIAFGRHTRVVLDIGCGVASFGA 300
           GQNWITRDKDKF+FPGGGTQFIHGADEYLDHISKMIPDIAFG HTRVVLDIGCGVASFGA
Sbjct: 241 GQNWITRDKDKFKFPGGGTQFIHGADEYLDHISKMIPDIAFGHHTRVVLDIGCGVASFGA 300

Query: 301 YLLSRNVVTMSIAPKDVHENQIQFALERGVPAMVAAFATHRLLYPSQAFDLIHCSRCRIN 360
           YLLSRNV+TMSIAPKDVHENQIQFALERGVPAMVAAFATHRLLYPSQAFDLIHCSRCRIN
Sbjct: 301 YLLSRNVITMSIAPKDVHENQIQFALERGVPAMVAAFATHRLLYPSQAFDLIHCSRCRIN 360

Query: 361 WTRDDGILLLEVNRMLRAGGYFAWAAQPVYKHEEVLEEQWEEMLNLTTRLCWGFVKKDGY 420
           WTRDDGILLLE+NRMLRAGGYFAWAAQPVYKHEEVLEEQWEEMLNLTTRLCW FVKKDGY
Sbjct: 361 WTRDDGILLLELNRMLRAGGYFAWAAQPVYKHEEVLEEQWEEMLNLTTRLCWEFVKKDGY 420

Query: 421 IAIWQKPLNNSCYLNREAGTKPPLCDQNDDPDRVWNADLKPCISRIPEDGYGANITVWPE 480
           IAIW+KPLNNSCYLNR+A TKPPLCDQ+DDPDRVWN DLK CISR+PEDGYGAN+TVWPE
Sbjct: 421 IAIWRKPLNNSCYLNRQAETKPPLCDQDDDPDRVWNVDLKTCISRLPEDGYGANVTVWPE 480

Query: 481 RLHTPPGRLQTIQYDAYISRNELFKAESKYWNEIIDSYVRAFHWKTFRLRNVMDMKAGFG 540
           RLHTPPGRLQTIQYDAYISRNELFKAESKYWNEIIDSYVRAFHWKTFRLRNVMDM+AGFG
Sbjct: 481 RLHTPPGRLQTIQYDAYISRNELFKAESKYWNEIIDSYVRAFHWKTFRLRNVMDMRAGFG 540

Query: 541 GFAAALIDLKLDCWVLNVVPVSGANTLPVIYDRGLIGVMHDWCEPFDTYPRTYDLLHAAG 600
           GFAAALIDLKLDCWVLNVVPVSG NTLPVIYDRGLIGVMHDWCEPFDTYPRTYDLLH+AG
Sbjct: 541 GFAAALIDLKLDCWVLNVVPVSGPNTLPVIYDRGLIGVMHDWCEPFDTYPRTYDLLHSAG 600

Query: 601 LFSVERKRCNMSTIMLEMDRILRPGGRVYIRDSVAVMDELQDIAKAMGWRANVRDTSEGP 660
           LFSVERKRCN+STIMLEMDRILRPGGRVYIRDSVAVMDELQ I KAMGW  NVRDTSEGP
Sbjct: 601 LFSVERKRCNISTIMLEMDRILRPGGRVYIRDSVAVMDELQGIGKAMGWHVNVRDTSEGP 660

Query: 661 HASYKIMMADKLLLNA 677
           HASYKIMMADKLL  A
Sbjct: 661 HASYKIMMADKLLTRA 673

BLAST of Lag0030320 vs. ExPASy Swiss-Prot
Match: O22285 (Probable methyltransferase PMT11 OS=Arabidopsis thaliana OX=3702 GN=At2g39750 PE=2 SV=1)

HSP 1 Score: 1072.4 bits (2772), Expect = 2.0e-312
Identity = 499/692 (72.11%), Postives = 571/692 (82.51%), Query Frame = 0

Query: 6   VVNGDSFRTPPLFKIVTFVLVSVTFFYFGKHWS-DGYQQLIFYPST---SQAPSVSLSPN 65
           + NGD F++P L KI   V V+V FFY GKHWS DGYQQL+F+ S+   S  P VS+SPN
Sbjct: 4   LTNGDLFKSPTLIKISALVFVTVAFFYLGKHWSDDGYQQLVFFSSSTSGSSIPEVSVSPN 63

Query: 66  YNKQFNISNLIDHNDTQTLPDKTLNLDP------TPSPLLGANPPPPPPPSDS------- 125
            N+ FN+S +I  N TQ     T+   P      T    + ANPPPPPPPS S       
Sbjct: 64  SNRVFNLSAIIPTNHTQIEIPATIRQQPPSVVADTEKVKVEANPPPPPPPSPSPPPPPGP 123

Query: 126 VQRFGIVDENGTMSDQFEVGDLDPEYVENWGNSTEV----DNGEGGARNFRIKKFGLCPQ 185
           V+ FGIVD NG MSD FEVG+++ + VE+WGN TE+     +G+  AR  RIKKFG+CP+
Sbjct: 124 VKSFGIVDANGVMSDDFEVGEVESDTVEDWGNQTEIVEAKSDGDSKAR-VRIKKFGMCPE 183

Query: 186 NMSEYIPCLDNAEAIAKLKSTEKGEKFERHCPVAGGGFDCLIPPPKGYQAPIPWPKSRDE 245
           +M EYIPCLDN + I KLKSTE+GE+FERHCP  G G +CL+PPPKGY+ PIPWPKSRDE
Sbjct: 184 SMREYIPCLDNTDVIKKLKSTERGERFERHCPEKGKGLNCLVPPPKGYRQPIPWPKSRDE 243

Query: 246 VWFSNVPHTRLVEDKGGQNWITRDKDKFRFPGGGTQFIHGADEYLDHISKMIPDIAFGRH 305
           VWFSNVPHTRLVEDKGGQNWI+RDK+KF+FPGGGTQFIHGAD+YLD +SKM+ DI FG+H
Sbjct: 244 VWFSNVPHTRLVEDKGGQNWISRDKNKFKFPGGGTQFIHGADQYLDQMSKMVSDITFGKH 303

Query: 306 TRVVLDIGCGVASFGAYLLSRNVVTMSIAPKDVHENQIQFALERGVPAMVAAFATHRLLY 365
            RV +D+GCGVASFGAYLLSR+V+TMS+APKDVHENQIQFALERGVPAM AAFAT RLLY
Sbjct: 304 IRVAMDVGCGVASFGAYLLSRDVMTMSVAPKDVHENQIQFALERGVPAMAAAFATRRLLY 363

Query: 366 PSQAFDLIHCSRCRINWTRDDGILLLEVNRMLRAGGYFAWAAQPVYKHEEVLEEQWEEML 425
           PSQAFDLIHCSRCRINWTRDDGILLLE+NRMLRAGGYFAWAAQPVYKHE  LEEQW EML
Sbjct: 364 PSQAFDLIHCSRCRINWTRDDGILLLEINRMLRAGGYFAWAAQPVYKHEPALEEQWTEML 423

Query: 426 NLTTRLCWGFVKKDGYIAIWQKPLNNSCYLNREAGTKPPLCDQNDDPDRVWNADLKPCIS 485
           NLT  LCW  VKK+GY+AIWQKP NN CYL+REAGTKPPLCD++DDPD VW  +LKPCIS
Sbjct: 424 NLTISLCWKLVKKEGYVAIWQKPFNNDCYLSREAGTKPPLCDESDDPDNVWYTNLKPCIS 483

Query: 486 RIPEDGYGANITVWPERLHTPPGRLQTIQYDAYISRNELFKAESKYWNEIIDSYVRAFHW 545
           RIPE GYG N+ +WP RLHTPP RLQTI++D+YI+R ELFKAESKYWNEII  YVRA  W
Sbjct: 484 RIPEKGYGGNVPLWPARLHTPPDRLQTIKFDSYIARKELFKAESKYWNEIIGGYVRALKW 543

Query: 546 KTFRLRNVMDMKAGFGGFAAALIDLKLDCWVLNVVPVSGANTLPVIYDRGLIGVMHDWCE 605
           K  +LRNV+DM+AGFGGFAAAL D KLDCWVL+VVPVSG NTLPVIYDRGL+GVMHDWCE
Sbjct: 544 KKMKLRNVLDMRAGFGGFAAALNDHKLDCWVLSVVPVSGPNTLPVIYDRGLLGVMHDWCE 603

Query: 606 PFDTYPRTYDLLHAAGLFSVERKRCNMSTIMLEMDRILRPGGRVYIRDSVAVMDELQDIA 665
           PFDTYPRTYD LHA+GLFS+ERKRC MSTI+LEMDRILRPGGR YIRDS+ VMDE+Q+I 
Sbjct: 604 PFDTYPRTYDFLHASGLFSIERKRCEMSTILLEMDRILRPGGRAYIRDSIDVMDEIQEIT 663

Query: 666 KAMGWRANVRDTSEGPHASYKIMMADKLLLNA 677
           KAMGW  ++RDTSEGPHASY+I+  +K LL A
Sbjct: 664 KAMGWHTSLRDTSEGPHASYRILTCEKRLLRA 694

BLAST of Lag0030320 vs. ExPASy Swiss-Prot
Match: Q9FG39 (Probable methyltransferase PMT12 OS=Arabidopsis thaliana OX=3702 GN=At5g06050 PE=2 SV=1)

HSP 1 Score: 994.6 bits (2570), Expect = 5.5e-289
Identity = 461/672 (68.60%), Postives = 546/672 (81.25%), Query Frame = 0

Query: 7   VNGDSFRTPPLFKIVTFVLVSVTFFYFGKHWS-DGYQQLIFYPS-TSQAPSVSLSPNYNK 66
           +N +  R    FKI  FVL+SV  F+ GKHWS DG+++LIF+ +  S++P V+LSP++ K
Sbjct: 5   LNSNLLRNSIFFKISAFVLISVACFFLGKHWSEDGFRRLIFFSAEPSRSPIVALSPDFGK 64

Query: 67  QFNISNLIDHNDTQTLPDKTLNLDPTPSPLLGANPPPPPPPSDSVQRFGIVDENGTMSDQ 126
            +NIS LI  +             P   P L    PPPPP S  ++ FGIV+ENGTMSD+
Sbjct: 65  TYNISGLIYES------------HPILPPSLS---PPPPPDSVELKVFGIVNENGTMSDE 124

Query: 127 FEVGDLDPEYVENWGNSTEVDNGE-----GGARNFRIKKFGLCPQNMSEYIPCLDNAEAI 186
           F++GD D E  E  GN TE ++ +            ++KF +C +NM+EYIPCLDN EAI
Sbjct: 125 FQIGDYDVESAETLGNQTEFESSDDDDIKSTTARVSVRKFEICSENMTEYIPCLDNVEAI 184

Query: 187 AKLKSTEKGEKFERHCPVAGGGFDCLIPPPKGYQAPIPWPKSRDEVWFSNVPHTRLVEDK 246
            +L ST +GE+FER+CP  G G +C +P P+GY++PIPWP+SRDEVWF+NVPHT+LVEDK
Sbjct: 185 KRLNSTARGERFERNCPNDGMGLNCTVPIPQGYRSPIPWPRSRDEVWFNNVPHTKLVEDK 244

Query: 247 GGQNWITRDKDKFRFPGGGTQFIHGADEYLDHISKMIPDIAFGRHTRVVLDIGCGVASFG 306
           GGQNWI ++ DKF+FPGGGTQFIHGAD+YLD IS+MIPDI+FG HTRVVLDIGCGVASFG
Sbjct: 245 GGQNWIYKENDKFKFPGGGTQFIHGADQYLDQISQMIPDISFGNHTRVVLDIGCGVASFG 304

Query: 307 AYLLSRNVVTMSIAPKDVHENQIQFALERGVPAMVAAFATHRLLYPSQAFDLIHCSRCRI 366
           AYL+SRNV+TMSIAPKDVHENQIQFALERGVPAMVAAF T RLLYPSQAFDL+HCSRCRI
Sbjct: 305 AYLMSRNVLTMSIAPKDVHENQIQFALERGVPAMVAAFTTRRLLYPSQAFDLVHCSRCRI 364

Query: 367 NWTRDDGILLLEVNRMLRAGGYFAWAAQPVYKHEEVLEEQWEEMLNLTTRLCWGFVKKDG 426
           NWTRDDGILLLEVNRMLRAGGYF WAAQPVYKHE+ LEEQWEEMLNLTTRLCW  VKK+G
Sbjct: 365 NWTRDDGILLLEVNRMLRAGGYFVWAAQPVYKHEKALEEQWEEMLNLTTRLCWVLVKKEG 424

Query: 427 YIAIWQKPLNNSCYLNREAGTKPPLCDQNDDPDRVWNADLKPCISRIPEDGYGANITVWP 486
           YIAIWQKP+NN+CYL+R AG  PPLC+  DDPD VW  DLK CI+RI E+GYGAN+  WP
Sbjct: 425 YIAIWQKPVNNTCYLSRGAGVSPPLCNSEDDPDNVWYVDLKACITRIEENGYGANLAPWP 484

Query: 487 ERLHTPPGRLQTIQYDAYISRNELFKAESKYWNEIIDSYVRAFHWKTFRLRNVMDMKAGF 546
            RL TPP RLQTIQ D+YI+R ELF AESKYW EII +YV A HWK   LRNV+DM+AGF
Sbjct: 485 ARLLTPPDRLQTIQIDSYIARKELFVAESKYWKEIISNYVNALHWKQIGLRNVLDMRAGF 544

Query: 547 GGFAAALIDLKLDCWVLNVVPVSGANTLPVIYDRGLIGVMHDWCEPFDTYPRTYDLLHAA 606
           GGFAAAL +LK+DCWVLNV+PVSG NTLPVIYDRGL+GVMHDWCEPFDTYPRTYDLLHAA
Sbjct: 545 GGFAAALAELKVDCWVLNVIPVSGPNTLPVIYDRGLLGVMHDWCEPFDTYPRTYDLLHAA 604

Query: 607 GLFSVERKRCNMSTIMLEMDRILRPGGRVYIRDSVAVMDELQDIAKAMGWRANVRDTSEG 666
           GLFS+ERKRCNM+T+MLEMDRILRPGGRVYIRD++ V  ELQ+I  AM W  ++R+T+EG
Sbjct: 605 GLFSIERKRCNMTTMMLEMDRILRPGGRVYIRDTINVTSELQEIGNAMRWHTSLRETAEG 661

Query: 667 PHASYKIMMADK 672
           PH+SY++++ +K
Sbjct: 665 PHSSYRVLLCEK 661

BLAST of Lag0030320 vs. ExPASy Swiss-Prot
Match: Q94KE1 (Probable methyltransferase PMT10 OS=Arabidopsis thaliana OX=3702 GN=At1g77260 PE=2 SV=1)

HSP 1 Score: 926.0 bits (2392), Expect = 2.4e-268
Identity = 428/670 (63.88%), Postives = 520/670 (77.61%), Query Frame = 0

Query: 5   SVVNGDSFRTPPLFKIVTFVLVSVTFFYFGKHWSDGYQQLIFYPSTSQAPSVSLSPNYNK 64
           S V  D  +TP L K++ F  +S++  +   H+SD +     YPS     S S       
Sbjct: 4   SDVGLDVVKTPRLVKLIAFAFLSISTIFLFNHFSDSFS----YPSLPFPISSS------- 63

Query: 65  QFNISNLIDHNDTQTLPDKTLNLDPTPSPLLGANPPPPPPPSDSVQRFGIVDENGTMSDQ 124
               SN+ +   T       +   P P P L  +PPP PP   +V R GI++ENG MSD 
Sbjct: 64  ----SNVTEAIQTNITSVAAVAPSPPPRPRLKISPPPLPP---TVVRTGIINENGAMSDS 123

Query: 125 FEVGDLDPEYVENWGNST---EVDNGEGGARNFRIKKFGLCPQNMSEYIPCLDNAEAIAK 184
           FE+G  DP+ ++   ++T    V+  E     F+I+K  LC +   +YIPCLDN E I +
Sbjct: 124 FEIGGFDPDSIDELKSATGNSSVEEKESPEVGFQIEKLKLCDKTKIDYIPCLDNEEEIKR 183

Query: 185 LKSTEKGEKFERHCPVAGGGFDCLIPPPKGYQAPIPWPKSRDEVWFSNVPHTRLVEDKGG 244
           L +T++GE +ERHCP      DCLIPPP GY+ PI WP+SRD++WF+NVPHTRLVEDKGG
Sbjct: 184 LNNTDRGENYERHCPKQ--SLDCLIPPPDGYKKPIQWPQSRDKIWFNNVPHTRLVEDKGG 243

Query: 245 QNWITRDKDKFRFPGGGTQFIHGADEYLDHISKMIPDIAFGRHTRVVLDIGCGVASFGAY 304
           QNWI R+KDKF FPGGGTQFIHGAD+YLD IS+MIPDI FG  TRV LDIGCGVASFGA+
Sbjct: 244 QNWIRREKDKFVFPGGGTQFIHGADQYLDQISQMIPDITFGSRTRVALDIGCGVASFGAF 303

Query: 305 LLSRNVVTMSIAPKDVHENQIQFALERGVPAMVAAFATHRLLYPSQAFDLIHCSRCRINW 364
           L+ RN  T+S+APKDVHENQIQFALERGVPAMVA FAT RLLYPSQ+F++IHCSRCRINW
Sbjct: 304 LMQRNTTTLSVAPKDVHENQIQFALERGVPAMVAVFATRRLLYPSQSFEMIHCSRCRINW 363

Query: 365 TRDDGILLLEVNRMLRAGGYFAWAAQPVYKHEEVLEEQWEEMLNLTTRLCWGFVKKDGYI 424
           TRDDGILLLEVNRMLRAGGYF WAAQPVYKHE+ L+EQW+EML+LT R+CW  +KK+GYI
Sbjct: 364 TRDDGILLLEVNRMLRAGGYFVWAAQPVYKHEDNLQEQWKEMLDLTNRICWELIKKEGYI 423

Query: 425 AIWQKPLNNSCYLNREAGTKPPLCDQNDDPDRVWNADLKPCISRIPEDGYGANITVWPER 484
           A+W+KPLNNSCY++REAGTKPPLC  +DDPD VW  D+KPCI+R+P++GYGAN++ WP R
Sbjct: 424 AVWRKPLNNSCYVSREAGTKPPLCRPDDDPDDVWYVDMKPCITRLPDNGYGANVSTWPAR 483

Query: 485 LHTPPGRLQTIQYDAYISRNELFKAESKYWNEIIDSYVRAFHWKTFRLRNVMDMKAGFGG 544
           LH PP RLQ+IQ DAYISR E+ KAES++W E+++SYVR F WK F+LRNV+DM+AGFGG
Sbjct: 484 LHDPPERLQSIQMDAYISRKEIMKAESRFWLEVVESYVRVFRWKEFKLRNVLDMRAGFGG 543

Query: 545 FAAALIDLKLDCWVLNVVPVSGANTLPVIYDRGLIGVMHDWCEPFDTYPRTYDLLHAAGL 604
           FAAAL DL LDCWV+N+VPVSG NTLPVIYDRGL G MHDWCEPFDTYPRTYDL+HAA L
Sbjct: 544 FAAALNDLGLDCWVMNIVPVSGFNTLPVIYDRGLQGAMHDWCEPFDTYPRTYDLIHAAFL 603

Query: 605 FSVERKRCNMSTIMLEMDRILRPGGRVYIRDSVAVMDELQDIAKAMGWRANVRDTSEGPH 664
           FSVE+KRCN++ IMLEMDR+LRPGG VYIRDS+++MD+LQ +AKA+GW A V DT EGPH
Sbjct: 604 FSVEKKRCNITNIMLEMDRMLRPGGHVYIRDSLSLMDQLQQVAKAIGWTAGVHDTGEGPH 653

Query: 665 ASYKIMMADK 672
           AS +I++ DK
Sbjct: 664 ASVRILICDK 653

BLAST of Lag0030320 vs. ExPASy Swiss-Prot
Match: Q93YV7 (Probable methyltransferase PMT3 OS=Arabidopsis thaliana OX=3702 GN=At4g14360 PE=2 SV=1)

HSP 1 Score: 516.5 bits (1329), Expect = 4.4e-145
Identity = 254/534 (47.57%), Postives = 339/534 (63.48%), Query Frame = 0

Query: 128 GDLDPEYVENWGNSTEVDNGEGGARNFRIKKFGLCPQNMSEYIPCLD-NAEAIAKLK-ST 187
           GD D    ++  +S  VD+G      F  + F +C    SE IPCLD N     +LK   
Sbjct: 51  GDDDDTKQDDTSSSFGVDDG------FTPRSFPVCDDRHSELIPCLDRNLIYQMRLKLDL 110

Query: 188 EKGEKFERHCPVAGGGFDCLIPPPKGYQAPIPWPKSRDEVWFSNVPHTRLVEDKGGQNWI 247
              E +ERHCP     F+CLIPPP GY+ PI WPKSRDEVW  N+PHT L  +K  QNW+
Sbjct: 111 SLMEHYERHCPPPERRFNCLIPPPNGYKVPIKWPKSRDEVWKVNIPHTHLAHEKSDQNWM 170

Query: 248 TRDKDKFRFPGGGTQFIHGADEYLDHISKMI--PD--IAFGRHTRVVLDIGCGVASFGAY 307
               DK  FPGGGT F +GAD+Y+  ++ M+  P+  +  G   R V D+GCGVASFG Y
Sbjct: 171 VVKGDKINFPGGGTHFHYGADKYIASMANMLNYPNNVLNNGGRLRTVFDVGCGVASFGGY 230

Query: 308 LLSRNVVTMSIAPKDVHENQIQFALERGVPAMVAAFATHRLLYPSQAFDLIHCSRCRINW 367
           LLS +++TMS+AP DVH+NQIQFALERG+PA +    T RL YPS++F+L HCSRCRI+W
Sbjct: 231 LLSSDILTMSLAPNDVHQNQIQFALERGIPASLGVLGTKRLPYPSRSFELSHCSRCRIDW 290

Query: 368 TRDDGILLLEVNRMLRAGGYFAWAAQPVYKHEEVLEEQWEEMLNLTTRLCWGFVKKDGYI 427
            + DGILLLE++R+LR GGYFA+++   Y  +E     W EM  L  R+CW    K    
Sbjct: 291 LQRDGILLLELDRVLRPGGYFAYSSPEAYAQDEEDLRIWREMSALVERMCWKIAAKRNQT 350

Query: 428 AIWQKPLNNSCYLNREAGTKPPLCDQNDDPDRVWNADLKPCISRIPEDGY---GANITVW 487
            IWQKPL N CYL RE GT+PPLC  ++DPD VW  +++ CI+   +  +   G+ +  W
Sbjct: 351 VIWQKPLTNDCYLEREPGTQPPLCRSDNDPDAVWGVNMEACITSYSDHDHKTKGSGLAPW 410

Query: 488 PERLHTPPGRLQTIQYDAYISRNELFKAESKYWNEIIDSY--VRAFHWKTFRLRNVMDMK 547
           P RL +PP RL    Y        +F+ +++ W + +D+Y  + +   ++  +RN+MDMK
Sbjct: 411 PARLTSPPPRLADFGYS-----TGMFEKDTELWRQRVDTYWDLLSPRIESDTVRNIMDMK 470

Query: 548 AGFGGFAAALIDLKLDCWVLNVVPVSGANTLPVIYDRGLIGVMHDWCEPFDTYPRTYDLL 607
           A  G FAAAL   + D WV+NVVP  G NTL +IYDRGL+G +H WCE F TYPRTYDLL
Sbjct: 471 ASMGSFAAAL--KEKDVWVMNVVPEDGPNTLKLIYDRGLMGAVHSWCEAFSTYPRTYDLL 530

Query: 608 HAAGLFS-VERKRCNMSTIMLEMDRILRPGGRVYIRDSVAVMDELQDIAKAMGW 650
           HA  + S +++K C+   ++LEMDRILRP G + IRD   V+D ++   KA+ W
Sbjct: 531 HAWDIISDIKKKGCSEVDLLLEMDRILRPSGFIIIRDKQRVVDFVKKYLKALHW 571

BLAST of Lag0030320 vs. ExPASy Swiss-Prot
Match: Q8H118 (Probable methyltransferase PMT1 OS=Arabidopsis thaliana OX=3702 GN=At3g23300 PE=2 SV=2)

HSP 1 Score: 512.7 bits (1319), Expect = 6.4e-144
Identity = 248/528 (46.97%), Postives = 336/528 (63.64%), Query Frame = 0

Query: 154 FRIKKFGLCPQNMSEYIPCLD-NAEAIAKLK-STEKGEKFERHCPVAGGGFDCLIPPPKG 213
           F  + F +C    SE IPCLD N     +LK      E +ERHCP     F+CLIPPP G
Sbjct: 74  FTPRSFPVCDDRHSELIPCLDRNLIYQMRLKLDLSLMEHYERHCPPPERRFNCLIPPPPG 133

Query: 214 YQAPIPWPKSRDEVWFSNVPHTRLVEDKGGQNWITRDKDKFRFPGGGTQFIHGADEYLDH 273
           Y+ PI WPKSRDEVW  N+PHT L  +K  QNW+    +K  FPGGGT F +GAD+Y+  
Sbjct: 134 YKIPIKWPKSRDEVWKVNIPHTHLAHEKSDQNWMVVKGEKINFPGGGTHFHYGADKYIAS 193

Query: 274 ISKMI--PD--IAFGRHTRVVLDIGCGVASFGAYLLSRNVVTMSIAPKDVHENQIQFALE 333
           ++ M+  P+  +  G   R  LD+GCGVASFG YLL+  ++TMS+AP DVH+NQIQFALE
Sbjct: 194 MANMLNFPNNVLNNGGRLRTFLDVGCGVASFGGYLLASEIMTMSLAPNDVHQNQIQFALE 253

Query: 334 RGVPAMVAAFATHRLLYPSQAFDLIHCSRCRINWTRDDGILLLEVNRMLRAGGYFAWAAQ 393
           RG+PA +    T RL YPS++F+L HCSRCRI+W + DGILLLE++R+LR GGYFA+++ 
Sbjct: 254 RGIPAYLGVLGTKRLPYPSRSFELAHCSRCRIDWLQRDGILLLELDRVLRPGGYFAYSSP 313

Query: 394 PVYKHEEVLEEQWEEMLNLTTRLCWGFVKKDGYIAIWQKPLNNSCYLNREAGTKPPLCDQ 453
             Y  +E     W EM  L  R+CW    K     IWQKPL N CYL RE GT+PPLC+ 
Sbjct: 314 EAYAQDEEDLRIWREMSALVGRMCWTIAAKRNQTVIWQKPLTNDCYLGREPGTQPPLCNS 373

Query: 454 NDDPDRVWNADLKPCISRIPEDGY---GANITVWPERLHTPPGRLQTIQYDAYISRNELF 513
           + DPD V+  +++ CI++  +  +   G+ +  WP RL +PP RL    Y       ++F
Sbjct: 374 DSDPDAVYGVNMEACITQYSDHDHKTKGSGLAPWPARLTSPPPRLADFGYS-----TDIF 433

Query: 514 KAESKYWNEIIDSYVRAFHWK--TFRLRNVMDMKAGFGGFAAALIDLKLDCWVLNVVPVS 573
           + +++ W + +D+Y      K  +  +RN+MDMKA  G FAAAL   + D WV+NVVP  
Sbjct: 434 EKDTETWRQRVDTYWDLLSPKIQSDTVRNIMDMKASMGSFAAAL--KEKDVWVMNVVPED 493

Query: 574 GANTLPVIYDRGLIGVMHDWCEPFDTYPRTYDLLHAAGLFS-VERKRCNMSTIMLEMDRI 633
           G NTL +IYDRGL+G +H WCE F TYPRTYDLLHA  + S ++++ C+   ++LEMDRI
Sbjct: 494 GPNTLKLIYDRGLMGAVHSWCEAFSTYPRTYDLLHAWDIISDIKKRGCSAEDLLLEMDRI 553

Query: 634 LRPGGRVYIRDSVAVMDELQDIAKAMGWRA-NVRDTSEGPHASYKIMM 669
           LRP G + IRD  +V+D ++   KA+ W A   +  SE    S  +++
Sbjct: 554 LRPSGFILIRDKQSVVDLVKKYLKALHWEAVETKTASESDQDSDNVIL 594

BLAST of Lag0030320 vs. ExPASy TrEMBL
Match: A0A6J1FGM3 (Methyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111445502 PE=3 SV=1)

HSP 1 Score: 1325.1 bits (3428), Expect = 0.0e+00
Identity = 620/676 (91.72%), Postives = 644/676 (95.27%), Query Frame = 0

Query: 1   MRSDSVVNGDSFRTPPLFKIVTFVLVSVTFFYFGKHWSDGYQQLIFYPSTSQAPSVSLSP 60
           MRSDS+VNGDSFR PPLFKI T V VSVTFFYFGKHWSDGYQQLIF+ STSQAP+VSLSP
Sbjct: 1   MRSDSLVNGDSFRAPPLFKIATLVFVSVTFFYFGKHWSDGYQQLIFFQSTSQAPTVSLSP 60

Query: 61  NYNKQFNISNLIDHNDTQTLPDKTLNLDPTPSPLLGANPPPPPPPSDSVQRFGIVDENGT 120
           NY+K FNIS LI  NDT+TLPDKTLNLDPTPSPLL    P PPPPSDSVQRFGIVDENGT
Sbjct: 61  NYDKHFNISTLIQQNDTETLPDKTLNLDPTPSPLLA---PNPPPPSDSVQRFGIVDENGT 120

Query: 121 MSDQFEVGDLDPEYVENWGNSTEVDNGEGGARNFRIKKFGLCPQNMSEYIPCLDNAEAIA 180
           MSDQFEVGDLDPEYVEN GNSTEVDNG+GG+R+FRIKKF LCPQNMSEYIPCLDN +AI+
Sbjct: 121 MSDQFEVGDLDPEYVENRGNSTEVDNGKGGSRSFRIKKFALCPQNMSEYIPCLDNVDAIS 180

Query: 181 KLKSTEKGEKFERHCPVAGGGFDCLIPPPKGYQAPIPWPKSRDEVWFSNVPHTRLVEDKG 240
           KLKS EKGEKFERHCPV GGGF+CLIPPPKGYQ PIPWP+SRD+VWFSNVPHTRLVEDKG
Sbjct: 181 KLKSVEKGEKFERHCPVTGGGFNCLIPPPKGYQTPIPWPRSRDQVWFSNVPHTRLVEDKG 240

Query: 241 GQNWITRDKDKFRFPGGGTQFIHGADEYLDHISKMIPDIAFGRHTRVVLDIGCGVASFGA 300
           GQNWITRDKDKF+FPGGGTQFIHGADEYLDHISKMIPDIAFG HTRVVLDIGCGVASFGA
Sbjct: 241 GQNWITRDKDKFKFPGGGTQFIHGADEYLDHISKMIPDIAFGHHTRVVLDIGCGVASFGA 300

Query: 301 YLLSRNVVTMSIAPKDVHENQIQFALERGVPAMVAAFATHRLLYPSQAFDLIHCSRCRIN 360
           YLLSRNV+TMSIAPKDVHENQIQFALERGVPAMVAAFATHRLLYPSQAFDLIHCSRCRIN
Sbjct: 301 YLLSRNVITMSIAPKDVHENQIQFALERGVPAMVAAFATHRLLYPSQAFDLIHCSRCRIN 360

Query: 361 WTRDDGILLLEVNRMLRAGGYFAWAAQPVYKHEEVLEEQWEEMLNLTTRLCWGFVKKDGY 420
           WTRDDGILLLE+NRMLRAGGYFAWAAQPVYKHEEVLEEQWEEMLNLTTRLCW FVKKDGY
Sbjct: 361 WTRDDGILLLELNRMLRAGGYFAWAAQPVYKHEEVLEEQWEEMLNLTTRLCWEFVKKDGY 420

Query: 421 IAIWQKPLNNSCYLNREAGTKPPLCDQNDDPDRVWNADLKPCISRIPEDGYGANITVWPE 480
           IAIW+KPLNNSCYLNR+A TKPPLCDQ+DDPDRVWN DLK CISR+PEDGYGAN+TVWPE
Sbjct: 421 IAIWRKPLNNSCYLNRQAETKPPLCDQDDDPDRVWNVDLKTCISRLPEDGYGANVTVWPE 480

Query: 481 RLHTPPGRLQTIQYDAYISRNELFKAESKYWNEIIDSYVRAFHWKTFRLRNVMDMKAGFG 540
           RLHTPPGRLQTIQYDAYISRNELFKAESKYWNEIIDSYVRAFHWKTFRLRNVMDM+AGFG
Sbjct: 481 RLHTPPGRLQTIQYDAYISRNELFKAESKYWNEIIDSYVRAFHWKTFRLRNVMDMRAGFG 540

Query: 541 GFAAALIDLKLDCWVLNVVPVSGANTLPVIYDRGLIGVMHDWCEPFDTYPRTYDLLHAAG 600
           GFAAALIDLKLDCWVLNVVPVSG NTLPVIYDRGLIGVMHDWCEPFDTYPRTYDLLH+AG
Sbjct: 541 GFAAALIDLKLDCWVLNVVPVSGPNTLPVIYDRGLIGVMHDWCEPFDTYPRTYDLLHSAG 600

Query: 601 LFSVERKRCNMSTIMLEMDRILRPGGRVYIRDSVAVMDELQDIAKAMGWRANVRDTSEGP 660
           LFSVERKRCN+STIMLEMDRILRPGGRVYIRDSVAVMDELQ I KAMGW  NVRDTSEGP
Sbjct: 601 LFSVERKRCNISTIMLEMDRILRPGGRVYIRDSVAVMDELQGIGKAMGWHVNVRDTSEGP 660

Query: 661 HASYKIMMADKLLLNA 677
           HASYKIMMADKLL  A
Sbjct: 661 HASYKIMMADKLLTRA 673

BLAST of Lag0030320 vs. ExPASy TrEMBL
Match: A0A6J1JW87 (Methyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111488919 PE=3 SV=1)

HSP 1 Score: 1322.4 bits (3421), Expect = 0.0e+00
Identity = 620/676 (91.72%), Postives = 643/676 (95.12%), Query Frame = 0

Query: 1   MRSDSVVNGDSFRTPPLFKIVTFVLVSVTFFYFGKHWSDGYQQLIFYPSTSQAPSVSLSP 60
           MRSDS+VNGDSFR PPLFKI T V VSVTFFYFGKHWSDGYQQLIF+ STSQAP+VSLSP
Sbjct: 1   MRSDSLVNGDSFRAPPLFKIATLVFVSVTFFYFGKHWSDGYQQLIFFQSTSQAPTVSLSP 60

Query: 61  NYNKQFNISNLIDHNDTQTLPDKTLNLDPTPSPLLGANPPPPPPPSDSVQRFGIVDENGT 120
           NYNK FN S LI  NDTQTLPDKTLNLDPTPSPLL    P PPPPSDSVQRFGIVDENGT
Sbjct: 61  NYNKHFNSSTLIPQNDTQTLPDKTLNLDPTPSPLLA---PNPPPPSDSVQRFGIVDENGT 120

Query: 121 MSDQFEVGDLDPEYVENWGNSTEVDNGEGGARNFRIKKFGLCPQNMSEYIPCLDNAEAIA 180
           MSDQFEVGDLDPEYVEN GNSTEVDNG+GG+R+FRIKKF LCPQNMSEYIPCLDN +AI+
Sbjct: 121 MSDQFEVGDLDPEYVENRGNSTEVDNGKGGSRSFRIKKFALCPQNMSEYIPCLDNVDAIS 180

Query: 181 KLKSTEKGEKFERHCPVAGGGFDCLIPPPKGYQAPIPWPKSRDEVWFSNVPHTRLVEDKG 240
           KL+S EKGEKFERHCPVAGGGF+CLIPPPKGYQ PIPWP+SRD+VWFSNVPHTRLVEDKG
Sbjct: 181 KLESVEKGEKFERHCPVAGGGFNCLIPPPKGYQTPIPWPRSRDQVWFSNVPHTRLVEDKG 240

Query: 241 GQNWITRDKDKFRFPGGGTQFIHGADEYLDHISKMIPDIAFGRHTRVVLDIGCGVASFGA 300
           GQNWITRDKDKF+FPGGGTQFIHGADEYLDHISKMIPDIAFG HTRVVLDIGCGVASFGA
Sbjct: 241 GQNWITRDKDKFKFPGGGTQFIHGADEYLDHISKMIPDIAFGHHTRVVLDIGCGVASFGA 300

Query: 301 YLLSRNVVTMSIAPKDVHENQIQFALERGVPAMVAAFATHRLLYPSQAFDLIHCSRCRIN 360
           YLLSRNV+TMSIAPKDVHENQIQFALERGVPAMVAAFATHRLLYPSQAFDLIHCSRCRIN
Sbjct: 301 YLLSRNVITMSIAPKDVHENQIQFALERGVPAMVAAFATHRLLYPSQAFDLIHCSRCRIN 360

Query: 361 WTRDDGILLLEVNRMLRAGGYFAWAAQPVYKHEEVLEEQWEEMLNLTTRLCWGFVKKDGY 420
           WTRDDGILLLE+NRMLRAGGYFAWAAQPVYKHEEVLEEQWEEMLNLTTRLCW FVKKDGY
Sbjct: 361 WTRDDGILLLELNRMLRAGGYFAWAAQPVYKHEEVLEEQWEEMLNLTTRLCWEFVKKDGY 420

Query: 421 IAIWQKPLNNSCYLNREAGTKPPLCDQNDDPDRVWNADLKPCISRIPEDGYGANITVWPE 480
           IAIWQKPLNNSCYLNR+A TKPPLCDQ+DDPDRVWN DLK CISR+PEDGYGA +TVWPE
Sbjct: 421 IAIWQKPLNNSCYLNRQAETKPPLCDQDDDPDRVWNVDLKTCISRLPEDGYGATVTVWPE 480

Query: 481 RLHTPPGRLQTIQYDAYISRNELFKAESKYWNEIIDSYVRAFHWKTFRLRNVMDMKAGFG 540
           RL+TPPGRLQTIQYDAYISRNELFKAESKYWNEIIDSYVRAFHWKTFRLRNVMDM+AGFG
Sbjct: 481 RLYTPPGRLQTIQYDAYISRNELFKAESKYWNEIIDSYVRAFHWKTFRLRNVMDMRAGFG 540

Query: 541 GFAAALIDLKLDCWVLNVVPVSGANTLPVIYDRGLIGVMHDWCEPFDTYPRTYDLLHAAG 600
           GFAAALIDLKLDCWVLNVVPVSG NTLPVIYDRGLIGVMHDWCEPFDTYPRTYDLLHAAG
Sbjct: 541 GFAAALIDLKLDCWVLNVVPVSGPNTLPVIYDRGLIGVMHDWCEPFDTYPRTYDLLHAAG 600

Query: 601 LFSVERKRCNMSTIMLEMDRILRPGGRVYIRDSVAVMDELQDIAKAMGWRANVRDTSEGP 660
           LFSVERKRCN+STIMLEMDR+LRPGGRVYIRDSVAVMDELQ I KAMGW  NVRDTSEGP
Sbjct: 601 LFSVERKRCNISTIMLEMDRMLRPGGRVYIRDSVAVMDELQGIGKAMGWHVNVRDTSEGP 660

Query: 661 HASYKIMMADKLLLNA 677
           HASYKIMMADKLL  A
Sbjct: 661 HASYKIMMADKLLTRA 673

BLAST of Lag0030320 vs. ExPASy TrEMBL
Match: A0A6J1HCT9 (Methyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111462808 PE=3 SV=1)

HSP 1 Score: 1321.2 bits (3418), Expect = 0.0e+00
Identity = 622/676 (92.01%), Postives = 641/676 (94.82%), Query Frame = 0

Query: 1   MRSDSVVNGDSFRTPPLFKIVTFVLVSVTFFYFGKHWSDGYQQLIFYPSTSQAPSVSLSP 60
           MRSDS+VN +SFR  PLFKI  F+L SVTFFYFGKHWSDGYQQL+FY  T QA SVSLSP
Sbjct: 1   MRSDSLVNAESFRASPLFKIAIFLLASVTFFYFGKHWSDGYQQLVFYTGTLQASSVSLSP 60

Query: 61  NYNKQFNISNLIDHNDTQTLPDKTLNLDPTPSPLLGANPPPPPPPSDSVQRFGIVDENGT 120
           NYNK FNISNLI  NDT  LPDKTLNLDPTP PLL  +  PPPPPSDSVQRFGI+DENGT
Sbjct: 61  NYNKHFNISNLIHQNDTHLLPDKTLNLDPTPPPLLRTD--PPPPPSDSVQRFGILDENGT 120

Query: 121 MSDQFEVGDLDPEYVENWGNSTEVDNGEGGARNFRIKKFGLCPQNMSEYIPCLDNAEAIA 180
           MSDQFEVGDLDPEYVENWGNSTEVD+GEG +RNFRIKKFGLCPQNMSEYIPCLDN EAIA
Sbjct: 121 MSDQFEVGDLDPEYVENWGNSTEVDHGEGSSRNFRIKKFGLCPQNMSEYIPCLDNQEAIA 180

Query: 181 KLKSTEKGEKFERHCPVAGGGFDCLIPPPKGYQAPIPWPKSRDEVWFSNVPHTRLVEDKG 240
           KLKSTEKGEKFERHCPVAGGGFDCLIPPPKGY+APIPWPKSRDEVWFSNVPHTRLVEDKG
Sbjct: 181 KLKSTEKGEKFERHCPVAGGGFDCLIPPPKGYRAPIPWPKSRDEVWFSNVPHTRLVEDKG 240

Query: 241 GQNWITRDKDKFRFPGGGTQFIHGADEYLDHISKMIPDIAFGRHTRVVLDIGCGVASFGA 300
           GQNWITRD DKFRFPGGGTQFIHGADEYLDHISKMIPDIAFG HTRVV+DIGCGVASFGA
Sbjct: 241 GQNWITRDNDKFRFPGGGTQFIHGADEYLDHISKMIPDIAFGHHTRVVMDIGCGVASFGA 300

Query: 301 YLLSRNVVTMSIAPKDVHENQIQFALERGVPAMVAAFATHRLLYPSQAFDLIHCSRCRIN 360
           YLLSR+VVTMSIAPKDVHENQIQFALERGVPAMVAAFATHRLLYPSQAFDLIHCSRCRIN
Sbjct: 301 YLLSRDVVTMSIAPKDVHENQIQFALERGVPAMVAAFATHRLLYPSQAFDLIHCSRCRIN 360

Query: 361 WTRDDGILLLEVNRMLRAGGYFAWAAQPVYKHEEVLEEQWEEMLNLTTRLCWGFVKKDGY 420
           WTRDDGILLLEVNRMLRAGGYFAWAAQPVYKHEEVLEEQW EMLNLTTRLCW FVKKDGY
Sbjct: 361 WTRDDGILLLEVNRMLRAGGYFAWAAQPVYKHEEVLEEQWGEMLNLTTRLCWEFVKKDGY 420

Query: 421 IAIWQKPLNNSCYLNREAGTKPPLCDQNDDPDRVWNADLKPCISRIPEDGYGANITVWPE 480
           IAIW+KPLNN+CYLNREAGTKPPLCDQNDDPDRVWN DLK CISR+PEDGYGANITVWP 
Sbjct: 421 IAIWRKPLNNTCYLNREAGTKPPLCDQNDDPDRVWNEDLKTCISRLPEDGYGANITVWPA 480

Query: 481 RLHTPPGRLQTIQYDAYISRNELFKAESKYWNEIIDSYVRAFHWKTFRLRNVMDMKAGFG 540
           RLHTPP RLQTIQYDAYISRNELFKAESKYWNEII+SYVRAFHWKTFRLRNVMDMKAGFG
Sbjct: 481 RLHTPPDRLQTIQYDAYISRNELFKAESKYWNEIIESYVRAFHWKTFRLRNVMDMKAGFG 540

Query: 541 GFAAALIDLKLDCWVLNVVPVSGANTLPVIYDRGLIGVMHDWCEPFDTYPRTYDLLHAAG 600
           GFAAALIDLKLDCWVLNVVPVSG NTLPVIYDRGL GVMHDWCEPFDTYPRTYDLLHAAG
Sbjct: 541 GFAAALIDLKLDCWVLNVVPVSGFNTLPVIYDRGLPGVMHDWCEPFDTYPRTYDLLHAAG 600

Query: 601 LFSVERKRCNMSTIMLEMDRILRPGGRVYIRDSVAVMDELQDIAKAMGWRANVRDTSEGP 660
           LFSVERKRCNMSTIMLEMDRILRPGGRVYIRDSVAV+DELQ IAKAMGWR +V DTSEGP
Sbjct: 601 LFSVERKRCNMSTIMLEMDRILRPGGRVYIRDSVAVIDELQHIAKAMGWRVDVHDTSEGP 660

Query: 661 HASYKIMMADKLLLNA 677
           HASYKIMMA+KLLL+A
Sbjct: 661 HASYKIMMANKLLLSA 674

BLAST of Lag0030320 vs. ExPASy TrEMBL
Match: A0A6J1JQA5 (Methyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111487363 PE=3 SV=1)

HSP 1 Score: 1317.8 bits (3409), Expect = 0.0e+00
Identity = 619/676 (91.57%), Postives = 642/676 (94.97%), Query Frame = 0

Query: 1   MRSDSVVNGDSFRTPPLFKIVTFVLVSVTFFYFGKHWSDGYQQLIFYPSTSQAPSVSLSP 60
           MRSDS+VN +SFRT PLFKI  F+L SVTFFYFGKHWSDGYQQL+FY  T QA SVSLSP
Sbjct: 1   MRSDSLVNAESFRTSPLFKIAIFLLASVTFFYFGKHWSDGYQQLVFYTGTIQASSVSLSP 60

Query: 61  NYNKQFNISNLIDHNDTQTLPDKTLNLDPTPSPLLGANPPPPPPPSDSVQRFGIVDENGT 120
           NY+K FNIS+LI  NDT  LPDKTLNLDPTP PLL  +  PPPPPSDSVQRFGI+D+NGT
Sbjct: 61  NYDKHFNISSLIHQNDTHVLPDKTLNLDPTPPPLLRTD--PPPPPSDSVQRFGILDKNGT 120

Query: 121 MSDQFEVGDLDPEYVENWGNSTEVDNGEGGARNFRIKKFGLCPQNMSEYIPCLDNAEAIA 180
           MSDQFEVGDLDPEYVENWGNSTEV +GEG +RNFRIKKFGLCPQNMSEYIPCLDN EAIA
Sbjct: 121 MSDQFEVGDLDPEYVENWGNSTEVHHGEGSSRNFRIKKFGLCPQNMSEYIPCLDNQEAIA 180

Query: 181 KLKSTEKGEKFERHCPVAGGGFDCLIPPPKGYQAPIPWPKSRDEVWFSNVPHTRLVEDKG 240
           KLKSTEKGEKFERHCPVAGGGFDCLIPPPKGY+APIPWPKSRDEVWFSNVPHTRLVEDKG
Sbjct: 181 KLKSTEKGEKFERHCPVAGGGFDCLIPPPKGYRAPIPWPKSRDEVWFSNVPHTRLVEDKG 240

Query: 241 GQNWITRDKDKFRFPGGGTQFIHGADEYLDHISKMIPDIAFGRHTRVVLDIGCGVASFGA 300
           GQNWITRDKDKFRFPGGGTQFIHGADEYLDHISKMIPDIAFG HTRVV+DIGCGVASFGA
Sbjct: 241 GQNWITRDKDKFRFPGGGTQFIHGADEYLDHISKMIPDIAFGHHTRVVMDIGCGVASFGA 300

Query: 301 YLLSRNVVTMSIAPKDVHENQIQFALERGVPAMVAAFATHRLLYPSQAFDLIHCSRCRIN 360
           YLLSR+VVTMSIAPKDVHENQIQFALERGVPAMVAAFATHRLLYPSQAFDLIHCSRCRIN
Sbjct: 301 YLLSRDVVTMSIAPKDVHENQIQFALERGVPAMVAAFATHRLLYPSQAFDLIHCSRCRIN 360

Query: 361 WTRDDGILLLEVNRMLRAGGYFAWAAQPVYKHEEVLEEQWEEMLNLTTRLCWGFVKKDGY 420
           WTRDDGILLLEVNRMLRAGGYFAWAAQPVYKHEEVLEEQW EMLNLTTRLCW FVKKDGY
Sbjct: 361 WTRDDGILLLEVNRMLRAGGYFAWAAQPVYKHEEVLEEQWGEMLNLTTRLCWEFVKKDGY 420

Query: 421 IAIWQKPLNNSCYLNREAGTKPPLCDQNDDPDRVWNADLKPCISRIPEDGYGANITVWPE 480
           IAIW+KPLNN+CYLNREAGTKPPLCDQNDDPDRVWN DLK CISR+PEDGYGANITVWP 
Sbjct: 421 IAIWRKPLNNTCYLNREAGTKPPLCDQNDDPDRVWNEDLKTCISRLPEDGYGANITVWPA 480

Query: 481 RLHTPPGRLQTIQYDAYISRNELFKAESKYWNEIIDSYVRAFHWKTFRLRNVMDMKAGFG 540
           RLHTPP RLQTIQYDAYISRNELFKAESKYWNEII+SYVRAFHWKTFRLRNVMDMKAGFG
Sbjct: 481 RLHTPPDRLQTIQYDAYISRNELFKAESKYWNEIIESYVRAFHWKTFRLRNVMDMKAGFG 540

Query: 541 GFAAALIDLKLDCWVLNVVPVSGANTLPVIYDRGLIGVMHDWCEPFDTYPRTYDLLHAAG 600
           GFAAALIDLKLDCWVLNVVPVSG NTLPVIYDRGL+GVMHDWCEPFDTYPRTYDLLHAAG
Sbjct: 541 GFAAALIDLKLDCWVLNVVPVSGFNTLPVIYDRGLLGVMHDWCEPFDTYPRTYDLLHAAG 600

Query: 601 LFSVERKRCNMSTIMLEMDRILRPGGRVYIRDSVAVMDELQDIAKAMGWRANVRDTSEGP 660
           LFSVERKRCNMSTIMLEMDRILRPGGRVYIRDSVAV+DELQ IAKAMGWR +V DTSEGP
Sbjct: 601 LFSVERKRCNMSTIMLEMDRILRPGGRVYIRDSVAVIDELQHIAKAMGWRVDVHDTSEGP 660

Query: 661 HASYKIMMADKLLLNA 677
           HASYKIMMA+K LL+A
Sbjct: 661 HASYKIMMANKFLLSA 674

BLAST of Lag0030320 vs. ExPASy TrEMBL
Match: A0A5D3DL40 (Methyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold205G00850 PE=3 SV=1)

HSP 1 Score: 1317.8 bits (3409), Expect = 0.0e+00
Identity = 618/681 (90.75%), Postives = 644/681 (94.57%), Query Frame = 0

Query: 1   MRSDSVVNGDSFRTPPLFKIVTFVLVSVTFFYFGKHWSDGYQQLIFYPST-----SQAPS 60
           MRSDS+ NGDSFR PP FKI++F+ V+VTFFYFGKHWSDGYQQLIF+ +T     S + S
Sbjct: 1   MRSDSLSNGDSFRFPPFFKILSFLFVAVTFFYFGKHWSDGYQQLIFFSTTATTQPSSSSS 60

Query: 61  VSLSPNYNKQFNISNLIDHNDTQTLPDKTLNLDPTPSPLLGANPPPPPPPSDSVQRFGIV 120
           VSLSPNYNK F+ISNLID NDTQT+PDKTLNLDP PSP    NPPPPPP  DSVQRFGIV
Sbjct: 61  VSLSPNYNKHFDISNLIDRNDTQTIPDKTLNLDPNPSPF---NPPPPPP--DSVQRFGIV 120

Query: 121 DENGTMSDQFEVGDLDPEYVENWGNSTEVDNGEGGARNFRIKKFGLCPQNMSEYIPCLDN 180
           DENGTMSDQFEVGD DPEYV+NWGNST+VDNG+GG R+FRIKKFGLCPQNMSEYIPCLDN
Sbjct: 121 DENGTMSDQFEVGDFDPEYVDNWGNSTQVDNGDGGTRSFRIKKFGLCPQNMSEYIPCLDN 180

Query: 181 AEAIAKLKSTEKGEKFERHCPVAGGGFDCLIPPPKGYQAPIPWPKSRDEVWFSNVPHTRL 240
           A+AIAKL+STE+GEKFERHCP AGGGFDCLIPPPKGYQ PIPWP+SRDEVWFSNVPHTRL
Sbjct: 181 ADAIAKLESTERGEKFERHCPDAGGGFDCLIPPPKGYQTPIPWPRSRDEVWFSNVPHTRL 240

Query: 241 VEDKGGQNWITRDKDKFRFPGGGTQFIHGADEYLDHISKMIPDIAFGRHTRVVLDIGCGV 300
           VEDKGGQNWITRDKDKFRFPGGGTQFIHGADEYLDHISKMIPDI FGRHTRVVLDIGCGV
Sbjct: 241 VEDKGGQNWITRDKDKFRFPGGGTQFIHGADEYLDHISKMIPDIVFGRHTRVVLDIGCGV 300

Query: 301 ASFGAYLLSRNVVTMSIAPKDVHENQIQFALERGVPAMVAAFATHRLLYPSQAFDLIHCS 360
           ASFGAYLLS+NV+TMSIAPKDVHENQIQFALERGVPAMVAAFATHRLLYPSQAFDLIHCS
Sbjct: 301 ASFGAYLLSQNVITMSIAPKDVHENQIQFALERGVPAMVAAFATHRLLYPSQAFDLIHCS 360

Query: 361 RCRINWTRDDGILLLEVNRMLRAGGYFAWAAQPVYKHEEVLEEQWEEMLNLTTRLCWGFV 420
           RCRINWTRDDGILLLEVNRMLRAGGYFAWAAQPVYKHEEVLEEQWEEMLNLTTRLCW FV
Sbjct: 361 RCRINWTRDDGILLLEVNRMLRAGGYFAWAAQPVYKHEEVLEEQWEEMLNLTTRLCWEFV 420

Query: 421 KKDGYIAIWQKPLNNSCYLNREAGTKPPLCDQNDDPDRVWNADLKPCISRIPEDGYGANI 480
           KKDGYIAIW+KPLNNSCYLNREA TKPPLCDQNDDPDRVWN +LK CISR+PEDGYG NI
Sbjct: 421 KKDGYIAIWRKPLNNSCYLNREAATKPPLCDQNDDPDRVWNVNLKTCISRLPEDGYGGNI 480

Query: 481 TVWPERLHTPPGRLQTIQYDAYISRNELFKAESKYWNEIIDSYVRAFHWKTFRLRNVMDM 540
           T WP RLHTPPGRLQTIQYDAYISRNELFKAESKYWNEIIDSYVRAFHWK+FRLRNVMDM
Sbjct: 481 TDWPARLHTPPGRLQTIQYDAYISRNELFKAESKYWNEIIDSYVRAFHWKSFRLRNVMDM 540

Query: 541 KAGFGGFAAALIDLKLDCWVLNVVPVSGANTLPVIYDRGLIGVMHDWCEPFDTYPRTYDL 600
           KAGFGGFAAAL+DLKLDCWVLNVVPVSG+NTLPVIYDRGLIGVMHDWCEPFDTYPRTYDL
Sbjct: 541 KAGFGGFAAALVDLKLDCWVLNVVPVSGSNTLPVIYDRGLIGVMHDWCEPFDTYPRTYDL 600

Query: 601 LHAAGLFSVERKRCNMSTIMLEMDRILRPGGRVYIRDSVAVMDELQDIAKAMGWRANVRD 660
           LHAAGLFSVERKRCNMSTIMLEMDRILRPGGRVYIRDSVAVMDELQDI KAMGW  NVRD
Sbjct: 601 LHAAGLFSVERKRCNMSTIMLEMDRILRPGGRVYIRDSVAVMDELQDIGKAMGWHVNVRD 660

Query: 661 TSEGPHASYKIMMADKLLLNA 677
           TSEGPHASYKIMMADKLLL A
Sbjct: 661 TSEGPHASYKIMMADKLLLKA 676

BLAST of Lag0030320 vs. TAIR 10
Match: AT2G39750.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 1072.4 bits (2772), Expect = 1.5e-313
Identity = 499/692 (72.11%), Postives = 571/692 (82.51%), Query Frame = 0

Query: 6   VVNGDSFRTPPLFKIVTFVLVSVTFFYFGKHWS-DGYQQLIFYPST---SQAPSVSLSPN 65
           + NGD F++P L KI   V V+V FFY GKHWS DGYQQL+F+ S+   S  P VS+SPN
Sbjct: 4   LTNGDLFKSPTLIKISALVFVTVAFFYLGKHWSDDGYQQLVFFSSSTSGSSIPEVSVSPN 63

Query: 66  YNKQFNISNLIDHNDTQTLPDKTLNLDP------TPSPLLGANPPPPPPPSDS------- 125
            N+ FN+S +I  N TQ     T+   P      T    + ANPPPPPPPS S       
Sbjct: 64  SNRVFNLSAIIPTNHTQIEIPATIRQQPPSVVADTEKVKVEANPPPPPPPSPSPPPPPGP 123

Query: 126 VQRFGIVDENGTMSDQFEVGDLDPEYVENWGNSTEV----DNGEGGARNFRIKKFGLCPQ 185
           V+ FGIVD NG MSD FEVG+++ + VE+WGN TE+     +G+  AR  RIKKFG+CP+
Sbjct: 124 VKSFGIVDANGVMSDDFEVGEVESDTVEDWGNQTEIVEAKSDGDSKAR-VRIKKFGMCPE 183

Query: 186 NMSEYIPCLDNAEAIAKLKSTEKGEKFERHCPVAGGGFDCLIPPPKGYQAPIPWPKSRDE 245
           +M EYIPCLDN + I KLKSTE+GE+FERHCP  G G +CL+PPPKGY+ PIPWPKSRDE
Sbjct: 184 SMREYIPCLDNTDVIKKLKSTERGERFERHCPEKGKGLNCLVPPPKGYRQPIPWPKSRDE 243

Query: 246 VWFSNVPHTRLVEDKGGQNWITRDKDKFRFPGGGTQFIHGADEYLDHISKMIPDIAFGRH 305
           VWFSNVPHTRLVEDKGGQNWI+RDK+KF+FPGGGTQFIHGAD+YLD +SKM+ DI FG+H
Sbjct: 244 VWFSNVPHTRLVEDKGGQNWISRDKNKFKFPGGGTQFIHGADQYLDQMSKMVSDITFGKH 303

Query: 306 TRVVLDIGCGVASFGAYLLSRNVVTMSIAPKDVHENQIQFALERGVPAMVAAFATHRLLY 365
            RV +D+GCGVASFGAYLLSR+V+TMS+APKDVHENQIQFALERGVPAM AAFAT RLLY
Sbjct: 304 IRVAMDVGCGVASFGAYLLSRDVMTMSVAPKDVHENQIQFALERGVPAMAAAFATRRLLY 363

Query: 366 PSQAFDLIHCSRCRINWTRDDGILLLEVNRMLRAGGYFAWAAQPVYKHEEVLEEQWEEML 425
           PSQAFDLIHCSRCRINWTRDDGILLLE+NRMLRAGGYFAWAAQPVYKHE  LEEQW EML
Sbjct: 364 PSQAFDLIHCSRCRINWTRDDGILLLEINRMLRAGGYFAWAAQPVYKHEPALEEQWTEML 423

Query: 426 NLTTRLCWGFVKKDGYIAIWQKPLNNSCYLNREAGTKPPLCDQNDDPDRVWNADLKPCIS 485
           NLT  LCW  VKK+GY+AIWQKP NN CYL+REAGTKPPLCD++DDPD VW  +LKPCIS
Sbjct: 424 NLTISLCWKLVKKEGYVAIWQKPFNNDCYLSREAGTKPPLCDESDDPDNVWYTNLKPCIS 483

Query: 486 RIPEDGYGANITVWPERLHTPPGRLQTIQYDAYISRNELFKAESKYWNEIIDSYVRAFHW 545
           RIPE GYG N+ +WP RLHTPP RLQTI++D+YI+R ELFKAESKYWNEII  YVRA  W
Sbjct: 484 RIPEKGYGGNVPLWPARLHTPPDRLQTIKFDSYIARKELFKAESKYWNEIIGGYVRALKW 543

Query: 546 KTFRLRNVMDMKAGFGGFAAALIDLKLDCWVLNVVPVSGANTLPVIYDRGLIGVMHDWCE 605
           K  +LRNV+DM+AGFGGFAAAL D KLDCWVL+VVPVSG NTLPVIYDRGL+GVMHDWCE
Sbjct: 544 KKMKLRNVLDMRAGFGGFAAALNDHKLDCWVLSVVPVSGPNTLPVIYDRGLLGVMHDWCE 603

Query: 606 PFDTYPRTYDLLHAAGLFSVERKRCNMSTIMLEMDRILRPGGRVYIRDSVAVMDELQDIA 665
           PFDTYPRTYD LHA+GLFS+ERKRC MSTI+LEMDRILRPGGR YIRDS+ VMDE+Q+I 
Sbjct: 604 PFDTYPRTYDFLHASGLFSIERKRCEMSTILLEMDRILRPGGRAYIRDSIDVMDEIQEIT 663

Query: 666 KAMGWRANVRDTSEGPHASYKIMMADKLLLNA 677
           KAMGW  ++RDTSEGPHASY+I+  +K LL A
Sbjct: 664 KAMGWHTSLRDTSEGPHASYRILTCEKRLLRA 694

BLAST of Lag0030320 vs. TAIR 10
Match: AT5G06050.1 (Putative methyltransferase family protein )

HSP 1 Score: 994.6 bits (2570), Expect = 3.9e-290
Identity = 461/672 (68.60%), Postives = 546/672 (81.25%), Query Frame = 0

Query: 7   VNGDSFRTPPLFKIVTFVLVSVTFFYFGKHWS-DGYQQLIFYPS-TSQAPSVSLSPNYNK 66
           +N +  R    FKI  FVL+SV  F+ GKHWS DG+++LIF+ +  S++P V+LSP++ K
Sbjct: 5   LNSNLLRNSIFFKISAFVLISVACFFLGKHWSEDGFRRLIFFSAEPSRSPIVALSPDFGK 64

Query: 67  QFNISNLIDHNDTQTLPDKTLNLDPTPSPLLGANPPPPPPPSDSVQRFGIVDENGTMSDQ 126
            +NIS LI  +             P   P L    PPPPP S  ++ FGIV+ENGTMSD+
Sbjct: 65  TYNISGLIYES------------HPILPPSLS---PPPPPDSVELKVFGIVNENGTMSDE 124

Query: 127 FEVGDLDPEYVENWGNSTEVDNGE-----GGARNFRIKKFGLCPQNMSEYIPCLDNAEAI 186
           F++GD D E  E  GN TE ++ +            ++KF +C +NM+EYIPCLDN EAI
Sbjct: 125 FQIGDYDVESAETLGNQTEFESSDDDDIKSTTARVSVRKFEICSENMTEYIPCLDNVEAI 184

Query: 187 AKLKSTEKGEKFERHCPVAGGGFDCLIPPPKGYQAPIPWPKSRDEVWFSNVPHTRLVEDK 246
            +L ST +GE+FER+CP  G G +C +P P+GY++PIPWP+SRDEVWF+NVPHT+LVEDK
Sbjct: 185 KRLNSTARGERFERNCPNDGMGLNCTVPIPQGYRSPIPWPRSRDEVWFNNVPHTKLVEDK 244

Query: 247 GGQNWITRDKDKFRFPGGGTQFIHGADEYLDHISKMIPDIAFGRHTRVVLDIGCGVASFG 306
           GGQNWI ++ DKF+FPGGGTQFIHGAD+YLD IS+MIPDI+FG HTRVVLDIGCGVASFG
Sbjct: 245 GGQNWIYKENDKFKFPGGGTQFIHGADQYLDQISQMIPDISFGNHTRVVLDIGCGVASFG 304

Query: 307 AYLLSRNVVTMSIAPKDVHENQIQFALERGVPAMVAAFATHRLLYPSQAFDLIHCSRCRI 366
           AYL+SRNV+TMSIAPKDVHENQIQFALERGVPAMVAAF T RLLYPSQAFDL+HCSRCRI
Sbjct: 305 AYLMSRNVLTMSIAPKDVHENQIQFALERGVPAMVAAFTTRRLLYPSQAFDLVHCSRCRI 364

Query: 367 NWTRDDGILLLEVNRMLRAGGYFAWAAQPVYKHEEVLEEQWEEMLNLTTRLCWGFVKKDG 426
           NWTRDDGILLLEVNRMLRAGGYF WAAQPVYKHE+ LEEQWEEMLNLTTRLCW  VKK+G
Sbjct: 365 NWTRDDGILLLEVNRMLRAGGYFVWAAQPVYKHEKALEEQWEEMLNLTTRLCWVLVKKEG 424

Query: 427 YIAIWQKPLNNSCYLNREAGTKPPLCDQNDDPDRVWNADLKPCISRIPEDGYGANITVWP 486
           YIAIWQKP+NN+CYL+R AG  PPLC+  DDPD VW  DLK CI+RI E+GYGAN+  WP
Sbjct: 425 YIAIWQKPVNNTCYLSRGAGVSPPLCNSEDDPDNVWYVDLKACITRIEENGYGANLAPWP 484

Query: 487 ERLHTPPGRLQTIQYDAYISRNELFKAESKYWNEIIDSYVRAFHWKTFRLRNVMDMKAGF 546
            RL TPP RLQTIQ D+YI+R ELF AESKYW EII +YV A HWK   LRNV+DM+AGF
Sbjct: 485 ARLLTPPDRLQTIQIDSYIARKELFVAESKYWKEIISNYVNALHWKQIGLRNVLDMRAGF 544

Query: 547 GGFAAALIDLKLDCWVLNVVPVSGANTLPVIYDRGLIGVMHDWCEPFDTYPRTYDLLHAA 606
           GGFAAAL +LK+DCWVLNV+PVSG NTLPVIYDRGL+GVMHDWCEPFDTYPRTYDLLHAA
Sbjct: 545 GGFAAALAELKVDCWVLNVIPVSGPNTLPVIYDRGLLGVMHDWCEPFDTYPRTYDLLHAA 604

Query: 607 GLFSVERKRCNMSTIMLEMDRILRPGGRVYIRDSVAVMDELQDIAKAMGWRANVRDTSEG 666
           GLFS+ERKRCNM+T+MLEMDRILRPGGRVYIRD++ V  ELQ+I  AM W  ++R+T+EG
Sbjct: 605 GLFSIERKRCNMTTMMLEMDRILRPGGRVYIRDTINVTSELQEIGNAMRWHTSLRETAEG 661

Query: 667 PHASYKIMMADK 672
           PH+SY++++ +K
Sbjct: 665 PHSSYRVLLCEK 661

BLAST of Lag0030320 vs. TAIR 10
Match: AT1G77260.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 926.0 bits (2392), Expect = 1.7e-269
Identity = 428/670 (63.88%), Postives = 520/670 (77.61%), Query Frame = 0

Query: 5   SVVNGDSFRTPPLFKIVTFVLVSVTFFYFGKHWSDGYQQLIFYPSTSQAPSVSLSPNYNK 64
           S V  D  +TP L K++ F  +S++  +   H+SD +     YPS     S S       
Sbjct: 4   SDVGLDVVKTPRLVKLIAFAFLSISTIFLFNHFSDSFS----YPSLPFPISSS------- 63

Query: 65  QFNISNLIDHNDTQTLPDKTLNLDPTPSPLLGANPPPPPPPSDSVQRFGIVDENGTMSDQ 124
               SN+ +   T       +   P P P L  +PPP PP   +V R GI++ENG MSD 
Sbjct: 64  ----SNVTEAIQTNITSVAAVAPSPPPRPRLKISPPPLPP---TVVRTGIINENGAMSDS 123

Query: 125 FEVGDLDPEYVENWGNST---EVDNGEGGARNFRIKKFGLCPQNMSEYIPCLDNAEAIAK 184
           FE+G  DP+ ++   ++T    V+  E     F+I+K  LC +   +YIPCLDN E I +
Sbjct: 124 FEIGGFDPDSIDELKSATGNSSVEEKESPEVGFQIEKLKLCDKTKIDYIPCLDNEEEIKR 183

Query: 185 LKSTEKGEKFERHCPVAGGGFDCLIPPPKGYQAPIPWPKSRDEVWFSNVPHTRLVEDKGG 244
           L +T++GE +ERHCP      DCLIPPP GY+ PI WP+SRD++WF+NVPHTRLVEDKGG
Sbjct: 184 LNNTDRGENYERHCPKQ--SLDCLIPPPDGYKKPIQWPQSRDKIWFNNVPHTRLVEDKGG 243

Query: 245 QNWITRDKDKFRFPGGGTQFIHGADEYLDHISKMIPDIAFGRHTRVVLDIGCGVASFGAY 304
           QNWI R+KDKF FPGGGTQFIHGAD+YLD IS+MIPDI FG  TRV LDIGCGVASFGA+
Sbjct: 244 QNWIRREKDKFVFPGGGTQFIHGADQYLDQISQMIPDITFGSRTRVALDIGCGVASFGAF 303

Query: 305 LLSRNVVTMSIAPKDVHENQIQFALERGVPAMVAAFATHRLLYPSQAFDLIHCSRCRINW 364
           L+ RN  T+S+APKDVHENQIQFALERGVPAMVA FAT RLLYPSQ+F++IHCSRCRINW
Sbjct: 304 LMQRNTTTLSVAPKDVHENQIQFALERGVPAMVAVFATRRLLYPSQSFEMIHCSRCRINW 363

Query: 365 TRDDGILLLEVNRMLRAGGYFAWAAQPVYKHEEVLEEQWEEMLNLTTRLCWGFVKKDGYI 424
           TRDDGILLLEVNRMLRAGGYF WAAQPVYKHE+ L+EQW+EML+LT R+CW  +KK+GYI
Sbjct: 364 TRDDGILLLEVNRMLRAGGYFVWAAQPVYKHEDNLQEQWKEMLDLTNRICWELIKKEGYI 423

Query: 425 AIWQKPLNNSCYLNREAGTKPPLCDQNDDPDRVWNADLKPCISRIPEDGYGANITVWPER 484
           A+W+KPLNNSCY++REAGTKPPLC  +DDPD VW  D+KPCI+R+P++GYGAN++ WP R
Sbjct: 424 AVWRKPLNNSCYVSREAGTKPPLCRPDDDPDDVWYVDMKPCITRLPDNGYGANVSTWPAR 483

Query: 485 LHTPPGRLQTIQYDAYISRNELFKAESKYWNEIIDSYVRAFHWKTFRLRNVMDMKAGFGG 544
           LH PP RLQ+IQ DAYISR E+ KAES++W E+++SYVR F WK F+LRNV+DM+AGFGG
Sbjct: 484 LHDPPERLQSIQMDAYISRKEIMKAESRFWLEVVESYVRVFRWKEFKLRNVLDMRAGFGG 543

Query: 545 FAAALIDLKLDCWVLNVVPVSGANTLPVIYDRGLIGVMHDWCEPFDTYPRTYDLLHAAGL 604
           FAAAL DL LDCWV+N+VPVSG NTLPVIYDRGL G MHDWCEPFDTYPRTYDL+HAA L
Sbjct: 544 FAAALNDLGLDCWVMNIVPVSGFNTLPVIYDRGLQGAMHDWCEPFDTYPRTYDLIHAAFL 603

Query: 605 FSVERKRCNMSTIMLEMDRILRPGGRVYIRDSVAVMDELQDIAKAMGWRANVRDTSEGPH 664
           FSVE+KRCN++ IMLEMDR+LRPGG VYIRDS+++MD+LQ +AKA+GW A V DT EGPH
Sbjct: 604 FSVEKKRCNITNIMLEMDRMLRPGGHVYIRDSLSLMDQLQQVAKAIGWTAGVHDTGEGPH 653

Query: 665 ASYKIMMADK 672
           AS +I++ DK
Sbjct: 664 ASVRILICDK 653

BLAST of Lag0030320 vs. TAIR 10
Match: AT4G14360.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 516.5 bits (1329), Expect = 3.1e-146
Identity = 254/534 (47.57%), Postives = 339/534 (63.48%), Query Frame = 0

Query: 128 GDLDPEYVENWGNSTEVDNGEGGARNFRIKKFGLCPQNMSEYIPCLD-NAEAIAKLK-ST 187
           GD D    ++  +S  VD+G      F  + F +C    SE IPCLD N     +LK   
Sbjct: 51  GDDDDTKQDDTSSSFGVDDG------FTPRSFPVCDDRHSELIPCLDRNLIYQMRLKLDL 110

Query: 188 EKGEKFERHCPVAGGGFDCLIPPPKGYQAPIPWPKSRDEVWFSNVPHTRLVEDKGGQNWI 247
              E +ERHCP     F+CLIPPP GY+ PI WPKSRDEVW  N+PHT L  +K  QNW+
Sbjct: 111 SLMEHYERHCPPPERRFNCLIPPPNGYKVPIKWPKSRDEVWKVNIPHTHLAHEKSDQNWM 170

Query: 248 TRDKDKFRFPGGGTQFIHGADEYLDHISKMI--PD--IAFGRHTRVVLDIGCGVASFGAY 307
               DK  FPGGGT F +GAD+Y+  ++ M+  P+  +  G   R V D+GCGVASFG Y
Sbjct: 171 VVKGDKINFPGGGTHFHYGADKYIASMANMLNYPNNVLNNGGRLRTVFDVGCGVASFGGY 230

Query: 308 LLSRNVVTMSIAPKDVHENQIQFALERGVPAMVAAFATHRLLYPSQAFDLIHCSRCRINW 367
           LLS +++TMS+AP DVH+NQIQFALERG+PA +    T RL YPS++F+L HCSRCRI+W
Sbjct: 231 LLSSDILTMSLAPNDVHQNQIQFALERGIPASLGVLGTKRLPYPSRSFELSHCSRCRIDW 290

Query: 368 TRDDGILLLEVNRMLRAGGYFAWAAQPVYKHEEVLEEQWEEMLNLTTRLCWGFVKKDGYI 427
            + DGILLLE++R+LR GGYFA+++   Y  +E     W EM  L  R+CW    K    
Sbjct: 291 LQRDGILLLELDRVLRPGGYFAYSSPEAYAQDEEDLRIWREMSALVERMCWKIAAKRNQT 350

Query: 428 AIWQKPLNNSCYLNREAGTKPPLCDQNDDPDRVWNADLKPCISRIPEDGY---GANITVW 487
            IWQKPL N CYL RE GT+PPLC  ++DPD VW  +++ CI+   +  +   G+ +  W
Sbjct: 351 VIWQKPLTNDCYLEREPGTQPPLCRSDNDPDAVWGVNMEACITSYSDHDHKTKGSGLAPW 410

Query: 488 PERLHTPPGRLQTIQYDAYISRNELFKAESKYWNEIIDSY--VRAFHWKTFRLRNVMDMK 547
           P RL +PP RL    Y        +F+ +++ W + +D+Y  + +   ++  +RN+MDMK
Sbjct: 411 PARLTSPPPRLADFGYS-----TGMFEKDTELWRQRVDTYWDLLSPRIESDTVRNIMDMK 470

Query: 548 AGFGGFAAALIDLKLDCWVLNVVPVSGANTLPVIYDRGLIGVMHDWCEPFDTYPRTYDLL 607
           A  G FAAAL   + D WV+NVVP  G NTL +IYDRGL+G +H WCE F TYPRTYDLL
Sbjct: 471 ASMGSFAAAL--KEKDVWVMNVVPEDGPNTLKLIYDRGLMGAVHSWCEAFSTYPRTYDLL 530

Query: 608 HAAGLFS-VERKRCNMSTIMLEMDRILRPGGRVYIRDSVAVMDELQDIAKAMGW 650
           HA  + S +++K C+   ++LEMDRILRP G + IRD   V+D ++   KA+ W
Sbjct: 531 HAWDIISDIKKKGCSEVDLLLEMDRILRPSGFIIIRDKQRVVDFVKKYLKALHW 571

BLAST of Lag0030320 vs. TAIR 10
Match: AT4G14360.2 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 516.5 bits (1329), Expect = 3.1e-146
Identity = 254/534 (47.57%), Postives = 339/534 (63.48%), Query Frame = 0

Query: 128 GDLDPEYVENWGNSTEVDNGEGGARNFRIKKFGLCPQNMSEYIPCLD-NAEAIAKLK-ST 187
           GD D    ++  +S  VD+G      F  + F +C    SE IPCLD N     +LK   
Sbjct: 51  GDDDDTKQDDTSSSFGVDDG------FTPRSFPVCDDRHSELIPCLDRNLIYQMRLKLDL 110

Query: 188 EKGEKFERHCPVAGGGFDCLIPPPKGYQAPIPWPKSRDEVWFSNVPHTRLVEDKGGQNWI 247
              E +ERHCP     F+CLIPPP GY+ PI WPKSRDEVW  N+PHT L  +K  QNW+
Sbjct: 111 SLMEHYERHCPPPERRFNCLIPPPNGYKVPIKWPKSRDEVWKVNIPHTHLAHEKSDQNWM 170

Query: 248 TRDKDKFRFPGGGTQFIHGADEYLDHISKMI--PD--IAFGRHTRVVLDIGCGVASFGAY 307
               DK  FPGGGT F +GAD+Y+  ++ M+  P+  +  G   R V D+GCGVASFG Y
Sbjct: 171 VVKGDKINFPGGGTHFHYGADKYIASMANMLNYPNNVLNNGGRLRTVFDVGCGVASFGGY 230

Query: 308 LLSRNVVTMSIAPKDVHENQIQFALERGVPAMVAAFATHRLLYPSQAFDLIHCSRCRINW 367
           LLS +++TMS+AP DVH+NQIQFALERG+PA +    T RL YPS++F+L HCSRCRI+W
Sbjct: 231 LLSSDILTMSLAPNDVHQNQIQFALERGIPASLGVLGTKRLPYPSRSFELSHCSRCRIDW 290

Query: 368 TRDDGILLLEVNRMLRAGGYFAWAAQPVYKHEEVLEEQWEEMLNLTTRLCWGFVKKDGYI 427
            + DGILLLE++R+LR GGYFA+++   Y  +E     W EM  L  R+CW    K    
Sbjct: 291 LQRDGILLLELDRVLRPGGYFAYSSPEAYAQDEEDLRIWREMSALVERMCWKIAAKRNQT 350

Query: 428 AIWQKPLNNSCYLNREAGTKPPLCDQNDDPDRVWNADLKPCISRIPEDGY---GANITVW 487
            IWQKPL N CYL RE GT+PPLC  ++DPD VW  +++ CI+   +  +   G+ +  W
Sbjct: 351 VIWQKPLTNDCYLEREPGTQPPLCRSDNDPDAVWGVNMEACITSYSDHDHKTKGSGLAPW 410

Query: 488 PERLHTPPGRLQTIQYDAYISRNELFKAESKYWNEIIDSY--VRAFHWKTFRLRNVMDMK 547
           P RL +PP RL    Y        +F+ +++ W + +D+Y  + +   ++  +RN+MDMK
Sbjct: 411 PARLTSPPPRLADFGYS-----TGMFEKDTELWRQRVDTYWDLLSPRIESDTVRNIMDMK 470

Query: 548 AGFGGFAAALIDLKLDCWVLNVVPVSGANTLPVIYDRGLIGVMHDWCEPFDTYPRTYDLL 607
           A  G FAAAL   + D WV+NVVP  G NTL +IYDRGL+G +H WCE F TYPRTYDLL
Sbjct: 471 ASMGSFAAAL--KEKDVWVMNVVPEDGPNTLKLIYDRGLMGAVHSWCEAFSTYPRTYDLL 530

Query: 608 HAAGLFS-VERKRCNMSTIMLEMDRILRPGGRVYIRDSVAVMDELQDIAKAMGW 650
           HA  + S +++K C+   ++LEMDRILRP G + IRD   V+D ++   KA+ W
Sbjct: 531 HAWDIISDIKKKGCSEVDLLLEMDRILRPSGFIIIRDKQRVVDFVKKYLKALHW 571

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038885726.10.0e+0092.76probable methyltransferase PMT11 [Benincasa hispida][more]
XP_023551343.10.0e+0092.01probable methyltransferase PMT11 [Cucurbita pepo subsp. pepo][more]
XP_023552424.10.0e+0092.01probable methyltransferase PMT11 [Cucurbita pepo subsp. pepo][more]
KAG6578419.10.0e+0091.72putative methyltransferase PMT11, partial [Cucurbita argyrosperma subsp. sororia... [more]
XP_022939686.10.0e+0091.72probable methyltransferase PMT11 [Cucurbita moschata] >KAG7015983.1 putative met... [more]
Match NameE-valueIdentityDescription
O222852.0e-31272.11Probable methyltransferase PMT11 OS=Arabidopsis thaliana OX=3702 GN=At2g39750 PE... [more]
Q9FG395.5e-28968.60Probable methyltransferase PMT12 OS=Arabidopsis thaliana OX=3702 GN=At5g06050 PE... [more]
Q94KE12.4e-26863.88Probable methyltransferase PMT10 OS=Arabidopsis thaliana OX=3702 GN=At1g77260 PE... [more]
Q93YV74.4e-14547.57Probable methyltransferase PMT3 OS=Arabidopsis thaliana OX=3702 GN=At4g14360 PE=... [more]
Q8H1186.4e-14446.97Probable methyltransferase PMT1 OS=Arabidopsis thaliana OX=3702 GN=At3g23300 PE=... [more]
Match NameE-valueIdentityDescription
A0A6J1FGM30.0e+0091.72Methyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111445502 PE=3 SV=1[more]
A0A6J1JW870.0e+0091.72Methyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111488919 PE=3 SV=1[more]
A0A6J1HCT90.0e+0092.01Methyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111462808 PE=3 SV=1[more]
A0A6J1JQA50.0e+0091.57Methyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111487363 PE=3 SV=1[more]
A0A5D3DL400.0e+0090.75Methyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold205G00... [more]
Match NameE-valueIdentityDescription
AT2G39750.11.5e-31372.11S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT5G06050.13.9e-29068.60Putative methyltransferase family protein [more]
AT1G77260.11.7e-26963.88S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT4G14360.13.1e-14647.57S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT4G14360.23.1e-14647.57S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.40.50.150Vaccinia Virus protein VP39coord: 265..424
e-value: 4.7E-12
score: 47.8
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 93..108
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 77..109
NoneNo IPR availablePANTHERPTHR10108:SF1091METHYLTRANSFERASE PMT12-RELATEDcoord: 16..672
NoneNo IPR availableCDDcd02440AdoMet_MTasescoord: 288..382
e-value: 0.00897327
score: 34.7131
IPR004159Putative S-adenosyl-L-methionine-dependent methyltransferasePFAMPF03141Methyltransf_29coord: 168..671
e-value: 1.2E-230
score: 766.4
IPR004159Putative S-adenosyl-L-methionine-dependent methyltransferasePANTHERPTHR10108SAM-DEPENDENT METHYLTRANSFERASEcoord: 16..672
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILY53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 505..648
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILY53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 204..411

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0030320.1Lag0030320.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0032259 methylation
cellular_component GO:0005737 cytoplasm
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0043231 intracellular membrane-bounded organelle
molecular_function GO:0008168 methyltransferase activity