Lag0030312 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0030312
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionWD_REPEATS_REGION domain-containing protein
Locationchr8: 46309813 .. 46314900 (+)
RNA-Seq ExpressionLag0030312
SyntenyLag0030312
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAATTCGGGTGATCAATCAATGGCATTACTGGCTGCAAGTGCTGGAGATACTGTCAAGCTTTTTGATGTCTCTGTTCATTCCAACGATCTGGGCGATCCGTGCACTTTGAGCTACACTCCTTCGCCGGGTTACCAGGTCAATTCAGTGAAGTGGAATCATACAAGTGAGTCGTTTTTAGATTCTTCCTCTCCCCCTTCAATTACCCTTTATCGGATTTTTGTCTTTTTGAGTTTCTTGGTGTTGGGCTTTGTCCAATTTCCAGCATTTCTTTTATTTCCTTTAATTTTCTTGAGTTATGCATTTGTGATTTTATAAAAATGGAAGTTTTGTTTTTGGTCTTTCGTATGGATTAAATTTCTGTTATCATGACGCTTAAGCATTTTAGACTTTTCCACCATTTGCTGATTGATAAACTTGATATTTTACTATTGAGATGGTGGTTCTGTTTGCTCTTGATTCAACTGGGATTTCATTTTTTGCAGATTTGGTCGTGGCCAGTGCCGGAGACGATAAGAAGATATCCTTGTGGCGTAAGAATGGTCAGAGCATGGGGACCATTCCAATTGCTGGTAGTGATAGTGGAGATAACATAGAGGTCAGTTAGTTGTTTAATGCAGTAGCATTTTATTTCATTTCGTTTCTTTTTTGTTCGGATATTAGGAGCTCAAGGAAATGTTCAGATGTGTTTTGAATTTTCACAACGGTTGAGTAATTGACTTTGAAAATAACATCCATCAATGCTGTACAAATTTATATTATTTTCCAATTATGTATGGCAAGTGATGATTAGTTGTTTAGCTTATACGCGGATAAACTTTTCTACAGGAATCTATATTTTCTATAAGTTTCAGCAACAAGGTATCCAGATACATGTGTACTGGAGGGAGTGGTCATGTTGTAAGAATATGGGATTTGCAGAGAAAGCGATGTATAAAATGGTTGAGAGGACATACCAATACTATAACAGGAGCTATGTACAATTGCAAAGATGAGCACCTTGCTTCAATCAGTCTTAGTGGGGATCTTATACTTCACAATCTTGCATCTGGTGCAAGGGCTGCTGAACTTAAAGACCCTAATGAACAGGTTGTCGAAGTTTATTGCATCTTAGCTGATTTTTAAGATTTACAGAGTCAGATGATCTGATTTTCAATTAATTGTCTTTTCTGATGGCTGTTAAGACCAGGTATTGAGGGTGCTTGACTATTCTCGTATTAGCAGACACCTTTTGGTGACTGCAGGTGATGATGGAACTGTACATCTATGGGATACAACTGGACGCAACCCTAAGGTTTGGATCAACTATCTTGTTGTAGGAATAACACAACTTGTTACTTATCGTTAAAATTTAACAAATCGATTTGGACAACATTTTTTGCGTCATTTATAATACCCACATCTTTTCATCTTTTAAGACATTTGAACTGCCTTGGTTTCTGTTGCAAGTCTTCGACAATGATGGATATATTCCATTTGGATCAATGGTTTCTATTGCTATTAGTAAGGCATTTTCTTCCGCAGGGGGGGGGGGGTGAGAGGATGTGGTAAGACTATTTGAAAACCACTCAAAATTTTGAAATCTTCCATTCAGTACTTCGAAGTATGAATAACCTTTTATCTTCATTTTTGTACTTAATAGAATATTTTTTTCAATGTTCCGACCATTATCCATGGCATTAATTTAATTTGCCCTGCCTCATTATATTTCACTATTTTCTGGTTTTGACATGTTTTGTGATGAACTTTATATTTCAGTCTGTTCTTTAGGTTTTATGTTTCACAGTGGCCTTTGTTTCGTAAGCTATGTTGCAATAATGGAGTTGCATACTTCTGTTTTGTTTAGGTTTCTTGGCAGAAACAACATTCTGCACCTACTGCTGGTATTGGCTTCTCACCATCTAATGATAAGGTCTCATTCTTATTCACGATTGATCAGTGATGTCTTGCTTTGGTATATTTTAGAATTTTATGATTAGTGGAAGCTAAAAACTTCTTAGGGTGAGAAGTTTTGTCCATACAAGTAAAATCTCTTTTCTTTTTTCTTTTTTTTTTCCTGATAGACTCTTGCTAGTGTTGGGCTGGATAAGAAGTTGTACACTTACGACTCAGGATCAAGGAGGCCATCTTCTTTCATTGCTTATGAGGCACCCTTCTCATCAATGGCTTTCAGAGATGATGGTTTGGTACTTGCTGCTGGAACAAGCAATGGACGTGTGGTTTTCTATGATGTCCGTGGGAAACCAGAGCCTTACATTGTTCTTCGTGCTTATAGTAGTTCAGAGGTATTCCCCCAGTATTTTGTAGAATCCTTGTCTGCTCCCTAAGTTGGTTTGCTTTGCTGGATCTTTCTTTACCATCATGTTATTGTGTGCTTTTAGGCTCATGAATCATAAGTTTTGTTCATCAATTTCATTTTTTTCCCATCATGGGCTCTTTTTTATTTTTTATTTGTGTAATACGTATGGTGCTAAGAGGAATTGGAAATAGCCATTGGAATGGTTTTTGTTTGTTCATGTGCTTATTAAGTTCAAACCACTCTAGCCCAATCTTGTTTCTTTCACTCATTATCACTGTTATCCGTAAGCAGTATTGATTGCATGCTAGGTTTACTAATTATTTGCAGTACAATCCAGGCAGTTACAAGTTTATCATGGCAGAGGTTAAAACCTGTAATTGTAAATGAAGGCAATTGCACTGCTGAGGTTGCTCTTTTGGGAGGTGCCGTTGAAGATTCAATTCTCATGCCTGACCCACTTCCATCTGTGACTACATCCACCGTTGCTCTGTCTACAACAGTATCCAGTTCTCGGAATCCTGGCCGTTCAGGTCCAACTTTCGAAGCTTCACTGACAGAAACTTCCAGTACATTCTCAACTGCAGAGGAAACACCACACCGTAGCCATCTGCGGCCAGGGGGAATGTTAGCTAGATTACATGCTCCTCGCTCCAGTTACAACTTCAAGGATGATATGGAGGTTTTTTCTCCTCTTGTGGATGTTCAGCCAATCACACCTTCATTGGACAAGCTGTGGGATGAACAAAATGGACAGAAGAAGGACCATCCATTTGACAAGAAACCTCTATCTTTGTTGTTTCCTTCATCAAGCAGAAGGTTCTCTTCTATTGAGGATGGGGCAAGTGATCATCCAATATTTAATTGGAAATCTTCATCTTCCAGACAGGTGATGCTAACCTCTTAACTGCAAGTTGTTTTTCATACGTGATAAAAGAAAGATCTTTCTGTGCTAGATGCACTGGTACTCTGCCATAATAAATGCTCATCATACTTGGTCATCTTCAGTATTCTAGATTACCTTTCAGCAATCTCTATTGCCTTGTAGTCTAGAGATATGAAAGCTAGATATAAATAGTGTTGATTGTTGAGGTATAACCTCAGTTTTGCTGTCATTTTTACTCAGGCGAGTGATAGAAATTATATTATTTATATGTCAGAATCACACATTTATCTCAATGGTGGTAGACCAAATATCCTTGACAAAATTATCAGATGACGTACATGGTATAATAGATTCAAACTAATTGTAATAGTTTTCTAAGAAGCACATATATTTTAGTTCATATAACATGTCTATGTTTGACGCATATTGGAAATTCCAACACTTTATAGCACAATAGGTATGTGTTAGATACTAGTTCTACAAAGTGAATATGACCTACCATTGTTATACACTTGTTAGGCACATATTGATAAGTATGCATTTATTTTTCACATTTGCGGTTATGATTTTCTGGCTCTTTTGATCGAACCAATGAATTCTAGTGAGAGCTTTCCTTTGAGGCCGTTGCACAGCATGGGAAATGGTGGTTTTGGTCACTAATACTCTGACTTTTTGTCAGGATGATTCTCGATCTTCTTCTGGACAATTGGGTTCTACTCCTGCTCCAACCCTAAATTCTAATAATGAAGACTCATCGATTACTCCTCCGGAAGCTTGGGGTGGTGAGAAATTATCTGATAAATTTGCCCAACTGAGGCAGCCAGTGTCTTTACCATCACGTTTTGGGATGATGGCTTCAGGTTCAAGTAGTCAGACATCAAGCTCAATGATTTCTGGATTACAAGATCCGTCCTCATCAGTTAGCCAAAGCAGCATCACTTCCTTGACAAATTTGAATTTCAGCTATCCAAATTTGCGCACAAAAGATGTAACCTCACAGGAGGTTTCATTGTCAATTCCTGAACACTTTTCTACTACTGCTGCATCTTTGTCTCTCGGTACCAGAGGAACCATTGGACTGAGCAACCAAGATTCACCTAGGAACACAACTATGACCCTGCCTCGCAGATTTTCTACATACGCGGAGAGGTTAAGCACTACTTCATCTTTCAGTGATGGACTTCCAATGGGGTCACCAAAAACAAAGAAAATGGGTTCTGAGACTAGGGAAGAGGTTTTGAATAACTTGTCAAAGTTCGATACGTTGTCAGTCACGGAATCAGGGATTCTTCCAGCGATAAACGTATGAATACTCTTCCAAGTTAAATGCTTTTATATGTTGTTTGTGACTCGGAATTTTGATTCTAGGTGTTACTAATTTTCTTTTAACTTTGTTGTCTACATTCTAGTGCTTTCTAATCATCATGCACATTTTATGGGGGTTACTTGCATGAAGGGTTGTCTCTTGAAATGGAAAATGTTGGTCTCTGATCTTACTATTGTTGGCAGGTAGGATCATTACAACCACAGAAGGCATTGCAATCAGATGCCCAACAAGGAAATTCATTTACACTTCAGCTTTTTCAACGCACTTTGGAAGAAACTCTCGACTCCTTTCAGAGATCCATTCACGATGACATGAGGAACCTTCATTTAGAGATTCTGAGACAGTTTCATATGCAGGAGGTACTTTATTTTAGATTCAATACGACCAAACTAGAACAGGGTTTCTGTCATTTACATTTCCTTTCCTCATGCTCTGTCTCATTTTTATCTCGAAATCTTTGCACAGATGGAAATGTCAAGTGTGATGAGCACCATTCTGGAAAATCAAGCCGAGCTGATCAAAGAGGTCAAGTCTCTAAGGAAAGAAAACCAGCAGCTTCGAGATTTGCTTGGATCCTAG

mRNA sequence

ATGAATTCGGGTGATCAATCAATGGCATTACTGGCTGCAAGTGCTGGAGATACTGTCAAGCTTTTTGATGTCTCTGTTCATTCCAACGATCTGGGCGATCCGTGCACTTTGAGCTACACTCCTTCGCCGGGTTACCAGGTCAATTCAGTGAAGTGGAATCATACAAATTTGGTCGTGGCCAGTGCCGGAGACGATAAGAAGATATCCTTGTGGCGTAAGAATGGTCAGAGCATGGGGACCATTCCAATTGCTGGTAGTGATAGTGGAGATAACATAGAGGAATCTATATTTTCTATAAGTTTCAGCAACAAGGTATCCAGATACATGTGTACTGGAGGGAGTGGTCATGTTGTAAGAATATGGGATTTGCAGAGAAAGCGATGTATAAAATGGTTGAGAGGACATACCAATACTATAACAGGAGCTATGTACAATTGCAAAGATGAGCACCTTGCTTCAATCAGTCTTAGTGGGGATCTTATACTTCACAATCTTGCATCTGGTGCAAGGGCTGCTGAACTTAAAGACCCTAATGAACAGGTATTGAGGGTGCTTGACTATTCTCGTATTAGCAGACACCTTTTGGTGACTGCAGGTGATGATGGAACTGTACATCTATGGGATACAACTGGACGCAACCCTAAGGTTTCTTGGCAGAAACAACATTCTGCACCTACTGCTGGTATTGGCTTCTCACCATCTAATGATAAGACTCTTGCTAGTGTTGGGCTGGATAAGAAGTTGTACACTTACGACTCAGGATCAAGGAGGCCATCTTCTTTCATTGCTTATGAGGCACCCTTCTCATCAATGGCTTTCAGAGATGATGGTTTGGTACTTGCTGCTGGAACAAGCAATGGACGTGTGGTTTTCTATGATGTCCGTGGGAAACCAGAGCCTTACATTGTTCTTCGTGCTTATAGTAGTTCAGAGGCAGTTACAAGTTTATCATGGCAGAGGTTAAAACCTGTAATTGTAAATGAAGGCAATTGCACTGCTGAGGTTGCTCTTTTGGGAGGTGCCGTTGAAGATTCAATTCTCATGCCTGACCCACTTCCATCTGTGACTACATCCACCGTTGCTCTGTCTACAACAGTATCCAGTTCTCGGAATCCTGGCCGTTCAGGTCCAACTTTCGAAGCTTCACTGACAGAAACTTCCAGTACATTCTCAACTGCAGAGGAAACACCACACCGTAGCCATCTGCGGCCAGGGGGAATGTTAGCTAGATTACATGCTCCTCGCTCCAGTTACAACTTCAAGGATGATATGGAGGTTTTTTCTCCTCTTGTGGATGTTCAGCCAATCACACCTTCATTGGACAAGCTGTGGGATGAACAAAATGGACAGAAGAAGGACCATCCATTTGACAAGAAACCTCTATCTTTGTTGTTTCCTTCATCAAGCAGAAGGTTCTCTTCTATTGAGGATGGGGCAAGTGATCATCCAATATTTAATTGGAAATCTTCATCTTCCAGACAGGATGATTCTCGATCTTCTTCTGGACAATTGGGTTCTACTCCTGCTCCAACCCTAAATTCTAATAATGAAGACTCATCGATTACTCCTCCGGAAGCTTGGGGTGGTGAGAAATTATCTGATAAATTTGCCCAACTGAGGCAGCCAGTGTCTTTACCATCACGTTTTGGGATGATGGCTTCAGGTTCAAGTAGTCAGACATCAAGCTCAATGATTTCTGGATTACAAGATCCGTCCTCATCAGTTAGCCAAAGCAGCATCACTTCCTTGACAAATTTGAATTTCAGCTATCCAAATTTGCGCACAAAAGATGTAACCTCACAGGAGGTTTCATTGTCAATTCCTGAACACTTTTCTACTACTGCTGCATCTTTGTCTCTCGGTACCAGAGGAACCATTGGACTGAGCAACCAAGATTCACCTAGGAACACAACTATGACCCTGCCTCGCAGATTTTCTACATACGCGGAGAGGTTAAGCACTACTTCATCTTTCAGTGATGGACTTCCAATGGGGTCACCAAAAACAAAGAAAATGGGTTCTGAGACTAGGGAAGAGGTTTTGAATAACTTGTCAAAGTTCGATACGTTGTCAGTCACGGAATCAGGGATTCTTCCAGCGATAAACGTAGGATCATTACAACCACAGAAGGCATTGCAATCAGATGCCCAACAAGGAAATTCATTTACACTTCAGCTTTTTCAACGCACTTTGGAAGAAACTCTCGACTCCTTTCAGAGATCCATTCACGATGACATGAGGAACCTTCATTTAGAGATTCTGAGACAGTTTCATATGCAGGAGATGGAAATGTCAAGTGTGATGAGCACCATTCTGGAAAATCAAGCCGAGCTGATCAAAGAGGTCAAGTCTCTAAGGAAAGAAAACCAGCAGCTTCGAGATTTGCTTGGATCCTAG

Coding sequence (CDS)

ATGAATTCGGGTGATCAATCAATGGCATTACTGGCTGCAAGTGCTGGAGATACTGTCAAGCTTTTTGATGTCTCTGTTCATTCCAACGATCTGGGCGATCCGTGCACTTTGAGCTACACTCCTTCGCCGGGTTACCAGGTCAATTCAGTGAAGTGGAATCATACAAATTTGGTCGTGGCCAGTGCCGGAGACGATAAGAAGATATCCTTGTGGCGTAAGAATGGTCAGAGCATGGGGACCATTCCAATTGCTGGTAGTGATAGTGGAGATAACATAGAGGAATCTATATTTTCTATAAGTTTCAGCAACAAGGTATCCAGATACATGTGTACTGGAGGGAGTGGTCATGTTGTAAGAATATGGGATTTGCAGAGAAAGCGATGTATAAAATGGTTGAGAGGACATACCAATACTATAACAGGAGCTATGTACAATTGCAAAGATGAGCACCTTGCTTCAATCAGTCTTAGTGGGGATCTTATACTTCACAATCTTGCATCTGGTGCAAGGGCTGCTGAACTTAAAGACCCTAATGAACAGGTATTGAGGGTGCTTGACTATTCTCGTATTAGCAGACACCTTTTGGTGACTGCAGGTGATGATGGAACTGTACATCTATGGGATACAACTGGACGCAACCCTAAGGTTTCTTGGCAGAAACAACATTCTGCACCTACTGCTGGTATTGGCTTCTCACCATCTAATGATAAGACTCTTGCTAGTGTTGGGCTGGATAAGAAGTTGTACACTTACGACTCAGGATCAAGGAGGCCATCTTCTTTCATTGCTTATGAGGCACCCTTCTCATCAATGGCTTTCAGAGATGATGGTTTGGTACTTGCTGCTGGAACAAGCAATGGACGTGTGGTTTTCTATGATGTCCGTGGGAAACCAGAGCCTTACATTGTTCTTCGTGCTTATAGTAGTTCAGAGGCAGTTACAAGTTTATCATGGCAGAGGTTAAAACCTGTAATTGTAAATGAAGGCAATTGCACTGCTGAGGTTGCTCTTTTGGGAGGTGCCGTTGAAGATTCAATTCTCATGCCTGACCCACTTCCATCTGTGACTACATCCACCGTTGCTCTGTCTACAACAGTATCCAGTTCTCGGAATCCTGGCCGTTCAGGTCCAACTTTCGAAGCTTCACTGACAGAAACTTCCAGTACATTCTCAACTGCAGAGGAAACACCACACCGTAGCCATCTGCGGCCAGGGGGAATGTTAGCTAGATTACATGCTCCTCGCTCCAGTTACAACTTCAAGGATGATATGGAGGTTTTTTCTCCTCTTGTGGATGTTCAGCCAATCACACCTTCATTGGACAAGCTGTGGGATGAACAAAATGGACAGAAGAAGGACCATCCATTTGACAAGAAACCTCTATCTTTGTTGTTTCCTTCATCAAGCAGAAGGTTCTCTTCTATTGAGGATGGGGCAAGTGATCATCCAATATTTAATTGGAAATCTTCATCTTCCAGACAGGATGATTCTCGATCTTCTTCTGGACAATTGGGTTCTACTCCTGCTCCAACCCTAAATTCTAATAATGAAGACTCATCGATTACTCCTCCGGAAGCTTGGGGTGGTGAGAAATTATCTGATAAATTTGCCCAACTGAGGCAGCCAGTGTCTTTACCATCACGTTTTGGGATGATGGCTTCAGGTTCAAGTAGTCAGACATCAAGCTCAATGATTTCTGGATTACAAGATCCGTCCTCATCAGTTAGCCAAAGCAGCATCACTTCCTTGACAAATTTGAATTTCAGCTATCCAAATTTGCGCACAAAAGATGTAACCTCACAGGAGGTTTCATTGTCAATTCCTGAACACTTTTCTACTACTGCTGCATCTTTGTCTCTCGGTACCAGAGGAACCATTGGACTGAGCAACCAAGATTCACCTAGGAACACAACTATGACCCTGCCTCGCAGATTTTCTACATACGCGGAGAGGTTAAGCACTACTTCATCTTTCAGTGATGGACTTCCAATGGGGTCACCAAAAACAAAGAAAATGGGTTCTGAGACTAGGGAAGAGGTTTTGAATAACTTGTCAAAGTTCGATACGTTGTCAGTCACGGAATCAGGGATTCTTCCAGCGATAAACGTAGGATCATTACAACCACAGAAGGCATTGCAATCAGATGCCCAACAAGGAAATTCATTTACACTTCAGCTTTTTCAACGCACTTTGGAAGAAACTCTCGACTCCTTTCAGAGATCCATTCACGATGACATGAGGAACCTTCATTTAGAGATTCTGAGACAGTTTCATATGCAGGAGATGGAAATGTCAAGTGTGATGAGCACCATTCTGGAAAATCAAGCCGAGCTGATCAAAGAGGTCAAGTCTCTAAGGAAAGAAAACCAGCAGCTTCGAGATTTGCTTGGATCCTAG

Protein sequence

MNSGDQSMALLAASAGDTVKLFDVSVHSNDLGDPCTLSYTPSPGYQVNSVKWNHTNLVVASAGDDKKISLWRKNGQSMGTIPIAGSDSGDNIEESIFSISFSNKVSRYMCTGGSGHVVRIWDLQRKRCIKWLRGHTNTITGAMYNCKDEHLASISLSGDLILHNLASGARAAELKDPNEQVLRVLDYSRISRHLLVTAGDDGTVHLWDTTGRNPKVSWQKQHSAPTAGIGFSPSNDKTLASVGLDKKLYTYDSGSRRPSSFIAYEAPFSSMAFRDDGLVLAAGTSNGRVVFYDVRGKPEPYIVLRAYSSSEAVTSLSWQRLKPVIVNEGNCTAEVALLGGAVEDSILMPDPLPSVTTSTVALSTTVSSSRNPGRSGPTFEASLTETSSTFSTAEETPHRSHLRPGGMLARLHAPRSSYNFKDDMEVFSPLVDVQPITPSLDKLWDEQNGQKKDHPFDKKPLSLLFPSSSRRFSSIEDGASDHPIFNWKSSSSRQDDSRSSSGQLGSTPAPTLNSNNEDSSITPPEAWGGEKLSDKFAQLRQPVSLPSRFGMMASGSSSQTSSSMISGLQDPSSSVSQSSITSLTNLNFSYPNLRTKDVTSQEVSLSIPEHFSTTAASLSLGTRGTIGLSNQDSPRNTTMTLPRRFSTYAERLSTTSSFSDGLPMGSPKTKKMGSETREEVLNNLSKFDTLSVTESGILPAINVGSLQPQKALQSDAQQGNSFTLQLFQRTLEETLDSFQRSIHDDMRNLHLEILRQFHMQEMEMSSVMSTILENQAELIKEVKSLRKENQQLRDLLGS
Homology
BLAST of Lag0030312 vs. NCBI nr
Match: XP_023537038.1 (protein NEDD1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1466.1 bits (3794), Expect = 0.0e+00
Identity = 753/800 (94.12%), Postives = 774/800 (96.75%), Query Frame = 0

Query: 1   MNSGDQSMALLAASAGDTVKLFDVSVHSNDLGDPCTLSYTPSPGYQVNSVKWNHTNLVVA 60
           M SGD SMALLAASAGDTVKLFDVS +SNDLGDPCTLSYTPSPGYQVNSVKWNHTNLVVA
Sbjct: 1   MTSGDPSMALLAASAGDTVKLFDVSAYSNDLGDPCTLSYTPSPGYQVNSVKWNHTNLVVA 60

Query: 61  SAGDDKKISLWRKNGQSMGTIPIAGSDSGDNIEESIFSISFSNKVSRYMCTGGSGHVVRI 120
           SAGDDKKISLWRKNGQSMGTIPIAGSD GDNIEESIFSISFSNKVSRYMCTGGSGHVVRI
Sbjct: 61  SAGDDKKISLWRKNGQSMGTIPIAGSDGGDNIEESIFSISFSNKVSRYMCTGGSGHVVRI 120

Query: 121 WDLQRKRCIKWLRGHTNTITGAMYNCKDEHLASISLSGDLILHNLASGARAAELKDPNEQ 180
           WDLQRKRCIKWLRGHTNTITGAMYNCKDEHLASISLSGDL+LHNLASGARAAELKDPNEQ
Sbjct: 121 WDLQRKRCIKWLRGHTNTITGAMYNCKDEHLASISLSGDLLLHNLASGARAAELKDPNEQ 180

Query: 181 VLRVLDYSRISRHLLVTAGDDGTVHLWDTTGRNPKVSWQKQHSAPTAGIGFSPSNDKTLA 240
           VLRVLDYSRISRHLLVTAGDDGTVHLWDTTGRNPKVSWQKQHSAPTAGI FSPSNDKTLA
Sbjct: 181 VLRVLDYSRISRHLLVTAGDDGTVHLWDTTGRNPKVSWQKQHSAPTAGISFSPSNDKTLA 240

Query: 241 SVGLDKKLYTYDSGSRRPSSFIAYEAPFSSMAFRDDGLVLAAGTSNGRVVFYDVRGKPEP 300
           SVGLDKKLYTYDSGSRRPSSFIAYEAPFSSMAFRDDGL+LAAGTSNGRVVFYDVRGKPEP
Sbjct: 241 SVGLDKKLYTYDSGSRRPSSFIAYEAPFSSMAFRDDGLILAAGTSNGRVVFYDVRGKPEP 300

Query: 301 YIVLRAYSSSEAVTSLSWQRLKPVIVNEGNCTAEVALLGGAVEDSILMPDPLPSVTTSTV 360
           YIVLRAYSSSEAVTS+SWQRLKPVIVNEGNCTA+VALLGGA+EDSILMPDPLPSVTTSTV
Sbjct: 301 YIVLRAYSSSEAVTSISWQRLKPVIVNEGNCTADVALLGGAIEDSILMPDPLPSVTTSTV 360

Query: 361 ALSTTVSSSRNPGRSGPTFEASLTETSSTFSTAEETPHRSHLRPGGMLARLHAPRSSYNF 420
           ALSTT SSSRNPG SG TFEASLTETSST STAEETPHRSHLRPGG L RLHAPRS+YNF
Sbjct: 361 ALSTTTSSSRNPGHSGLTFEASLTETSSTISTAEETPHRSHLRPGGSLTRLHAPRSNYNF 420

Query: 421 KDDMEVFSPLVDVQPITPSLDKLWDEQNGQKKDHPFDKKPLSLLFPSSSRRFSSIEDGAS 480
           KDDMEVFSPLVDVQPITPSLDKLWD+QNG KKDHPFDKKPL LLFPSSSRRFSSIEDG S
Sbjct: 421 KDDMEVFSPLVDVQPITPSLDKLWDDQNGLKKDHPFDKKPLPLLFPSSSRRFSSIEDGPS 480

Query: 481 DHPIFNWKSSSSRQDDSRSSSGQLGSTPAPTLNSNNEDSSITPPEAWGGEKLSDKFAQLR 540
           DHPIFNWKSSSS+QDDSRSSS QLGSTPAPTLNS NEDSSITPPEAWGGEKLSDKFAQLR
Sbjct: 481 DHPIFNWKSSSSKQDDSRSSSAQLGSTPAPTLNSKNEDSSITPPEAWGGEKLSDKFAQLR 540

Query: 541 QPVSLPSRFGMMASGSSSQTSSSMISGLQDPSSSVSQSSITSLTNLNFSYPNLRTKDVTS 600
           QP +LPSRFGM+AS S+SQTSSSMISGLQDPSSS+SQSSITSLTNLNFSYPN+RTKDV S
Sbjct: 541 QPATLPSRFGMLASSSNSQTSSSMISGLQDPSSSISQSSITSLTNLNFSYPNMRTKDVIS 600

Query: 601 QEVSLSIPEHFSTTAASLSLGTRGTIGLSNQDSPRNTTMTLPRRFSTYAERLSTTSSFSD 660
           QEVSLSIPEHFSTTAASLSLGTRGTIGLSNQDSPR TTMTLPRRFSTYAERLSTTSSFSD
Sbjct: 601 QEVSLSIPEHFSTTAASLSLGTRGTIGLSNQDSPRTTTMTLPRRFSTYAERLSTTSSFSD 660

Query: 661 GLP--MGSPKTKKMGSETREEVLNNLSKFDTLSVTESGILPAINVGSLQPQKALQSDAQQ 720
           GLP  +GSPKTKKMGSETREEVLNNLSKFDTLSVTESGILPA+N G LQ QKALQ+DAQQ
Sbjct: 661 GLPVGVGSPKTKKMGSETREEVLNNLSKFDTLSVTESGILPAMNGGPLQQQKALQTDAQQ 720

Query: 721 GNSFTLQLFQRTLEETLDSFQRSIHDDMRNLHLEILRQFHMQEMEMSSVMSTILENQAEL 780
           GNSFTLQLFQRTLEETLDSFQRSIHDDMRNLHLEILRQFHMQEMEMSSV S++L+NQAEL
Sbjct: 721 GNSFTLQLFQRTLEETLDSFQRSIHDDMRNLHLEILRQFHMQEMEMSSVTSSMLKNQAEL 780

Query: 781 IKEVKSLRKENQQLRDLLGS 799
           IKEVKSLRKENQQLR+LLGS
Sbjct: 781 IKEVKSLRKENQQLRNLLGS 800

BLAST of Lag0030312 vs. NCBI nr
Match: XP_022990911.1 (protein NEDD1 [Cucurbita maxima])

HSP 1 Score: 1465.7 bits (3793), Expect = 0.0e+00
Identity = 753/800 (94.12%), Postives = 773/800 (96.62%), Query Frame = 0

Query: 1   MNSGDQSMALLAASAGDTVKLFDVSVHSNDLGDPCTLSYTPSPGYQVNSVKWNHTNLVVA 60
           M SGD SMALLAASAGDTVKLFDVS +SNDLGDPCTLSYTPSPGYQVNSVKWNHTNLVVA
Sbjct: 1   MTSGDPSMALLAASAGDTVKLFDVSAYSNDLGDPCTLSYTPSPGYQVNSVKWNHTNLVVA 60

Query: 61  SAGDDKKISLWRKNGQSMGTIPIAGSDSGDNIEESIFSISFSNKVSRYMCTGGSGHVVRI 120
           SAGDDKKISLWRKNGQSMGTIPIAGSD GDNIEESIFSISFSNKVSRYMCTGGSGHVVRI
Sbjct: 61  SAGDDKKISLWRKNGQSMGTIPIAGSDGGDNIEESIFSISFSNKVSRYMCTGGSGHVVRI 120

Query: 121 WDLQRKRCIKWLRGHTNTITGAMYNCKDEHLASISLSGDLILHNLASGARAAELKDPNEQ 180
           WDLQRKRCIKWLRGHTNTITGAMYNCKDEHLASISLSGDL+LHNLASGARAAELKDPNEQ
Sbjct: 121 WDLQRKRCIKWLRGHTNTITGAMYNCKDEHLASISLSGDLLLHNLASGARAAELKDPNEQ 180

Query: 181 VLRVLDYSRISRHLLVTAGDDGTVHLWDTTGRNPKVSWQKQHSAPTAGIGFSPSNDKTLA 240
           VLRVLDYSRISRHLLVTAGDDGTVHLWDTTGRNPKVSWQKQHSAPTAGI FSPSNDKTLA
Sbjct: 181 VLRVLDYSRISRHLLVTAGDDGTVHLWDTTGRNPKVSWQKQHSAPTAGISFSPSNDKTLA 240

Query: 241 SVGLDKKLYTYDSGSRRPSSFIAYEAPFSSMAFRDDGLVLAAGTSNGRVVFYDVRGKPEP 300
           SVGLDKKLYTYDSGSRRPSSFIAYEAPFSSMAFRDDGL+LAAGTSNGRVVFYDVRGKPEP
Sbjct: 241 SVGLDKKLYTYDSGSRRPSSFIAYEAPFSSMAFRDDGLILAAGTSNGRVVFYDVRGKPEP 300

Query: 301 YIVLRAYSSSEAVTSLSWQRLKPVIVNEGNCTAEVALLGGAVEDSILMPDPLPSVTTSTV 360
           YIVLRAYSSSEAVTS+SWQRLKPV VNEGNCTA+VALLGGA+EDSILMPDPLPSVTTSTV
Sbjct: 301 YIVLRAYSSSEAVTSISWQRLKPVNVNEGNCTADVALLGGAIEDSILMPDPLPSVTTSTV 360

Query: 361 ALSTTVSSSRNPGRSGPTFEASLTETSSTFSTAEETPHRSHLRPGGMLARLHAPRSSYNF 420
           ALSTT SSSRNPG SG TFEASLTET ST STAEETPHRSHLRPGG L RLHAPRS+YNF
Sbjct: 361 ALSTTTSSSRNPGHSGLTFEASLTETCSTISTAEETPHRSHLRPGGSLTRLHAPRSNYNF 420

Query: 421 KDDMEVFSPLVDVQPITPSLDKLWDEQNGQKKDHPFDKKPLSLLFPSSSRRFSSIEDGAS 480
           KDDMEVFSPLVDVQPITPSLDKLWD+QNG KKDHPFDKKPL LLFPSSSRR SSIEDG S
Sbjct: 421 KDDMEVFSPLVDVQPITPSLDKLWDDQNGLKKDHPFDKKPLPLLFPSSSRRISSIEDGPS 480

Query: 481 DHPIFNWKSSSSRQDDSRSSSGQLGSTPAPTLNSNNEDSSITPPEAWGGEKLSDKFAQLR 540
           DHPIFNWKSSSS+QDDSRSSSGQLGSTPAPTLNS NEDSSITPPEAWGGEKLSDKFAQLR
Sbjct: 481 DHPIFNWKSSSSKQDDSRSSSGQLGSTPAPTLNSKNEDSSITPPEAWGGEKLSDKFAQLR 540

Query: 541 QPVSLPSRFGMMASGSSSQTSSSMISGLQDPSSSVSQSSITSLTNLNFSYPNLRTKDVTS 600
           QP +LPSRFGM+AS SSSQTSSSMISGLQDPSSS+SQSSITSLTNLNFSYPN+RTKDV S
Sbjct: 541 QPATLPSRFGMLASSSSSQTSSSMISGLQDPSSSISQSSITSLTNLNFSYPNMRTKDVIS 600

Query: 601 QEVSLSIPEHFSTTAASLSLGTRGTIGLSNQDSPRNTTMTLPRRFSTYAERLSTTSSFSD 660
           QEVSLSIPEHFSTTAASLSLGTRGTIGLSNQDSPR TTMTLPRRFSTYAERLSTTSSFSD
Sbjct: 601 QEVSLSIPEHFSTTAASLSLGTRGTIGLSNQDSPRTTTMTLPRRFSTYAERLSTTSSFSD 660

Query: 661 GLP--MGSPKTKKMGSETREEVLNNLSKFDTLSVTESGILPAINVGSLQPQKALQSDAQQ 720
           GLP  +GSPKTKKMGSETREEVLNNLSKFDTLSVTESGILPA+N G LQPQKALQ+DAQQ
Sbjct: 661 GLPVGVGSPKTKKMGSETREEVLNNLSKFDTLSVTESGILPAMNGGPLQPQKALQTDAQQ 720

Query: 721 GNSFTLQLFQRTLEETLDSFQRSIHDDMRNLHLEILRQFHMQEMEMSSVMSTILENQAEL 780
           GNSFTLQLFQRTLEETLDSFQRSIHDDMRNLHLEILRQFHMQEMEMSSV S++L+NQAEL
Sbjct: 721 GNSFTLQLFQRTLEETLDSFQRSIHDDMRNLHLEILRQFHMQEMEMSSVTSSMLKNQAEL 780

Query: 781 IKEVKSLRKENQQLRDLLGS 799
           IKEVKSLRKENQQLR+LLGS
Sbjct: 781 IKEVKSLRKENQQLRNLLGS 800

BLAST of Lag0030312 vs. NCBI nr
Match: XP_038886298.1 (protein NEDD1 isoform X1 [Benincasa hispida])

HSP 1 Score: 1462.2 bits (3784), Expect = 0.0e+00
Identity = 752/800 (94.00%), Postives = 776/800 (97.00%), Query Frame = 0

Query: 1   MNSGDQSMALLAASAGDTVKLFDVSVHSNDLGDPCTLSYTPSPGYQVNSVKWNHTNLVVA 60
           MN GD SMALLAASAGDTVKLFDVSVHSNDLGDPCTLSYTPSPG+QVNSVKWNHTNLVVA
Sbjct: 1   MNLGDPSMALLAASAGDTVKLFDVSVHSNDLGDPCTLSYTPSPGFQVNSVKWNHTNLVVA 60

Query: 61  SAGDDKKISLWRKNGQSMGTIPIAGSDSGDNIEESIFSISFSNKVSRYMCTGGSGHVVRI 120
           SAGDDKKISLWRKNGQSMGTIPIAG+DSGDNIEESIFSISFSNKVSRYMCTGGSGHVVRI
Sbjct: 61  SAGDDKKISLWRKNGQSMGTIPIAGTDSGDNIEESIFSISFSNKVSRYMCTGGSGHVVRI 120

Query: 121 WDLQRKRCIKWLRGHTNTITGAMYNCKDEHLASISLSGDLILHNLASGARAAELKDPNEQ 180
           WDLQRKRCIKWLRGHTNTITGAMYNCKDEHLASISLSGDLILHNLASGARAAELKDPNEQ
Sbjct: 121 WDLQRKRCIKWLRGHTNTITGAMYNCKDEHLASISLSGDLILHNLASGARAAELKDPNEQ 180

Query: 181 VLRVLDYSRISRHLLVTAGDDGTVHLWDTTGRNPKVSWQKQHSAPTAGIGFSPSNDKTLA 240
           VLRVLDYSR SRHLLVTAGDDGTVHLWDTTGRNPKVSWQKQHSAP AGIGFSPSNDKTLA
Sbjct: 181 VLRVLDYSRTSRHLLVTAGDDGTVHLWDTTGRNPKVSWQKQHSAPAAGIGFSPSNDKTLA 240

Query: 241 SVGLDKKLYTYDSGSRRPSSFIAYEAPFSSMAFRDDGLVLAAGTSNGRVVFYDVRGKPEP 300
           SVGLDKKLYTYDSGSRRPSSF+AYEAPFSSMAFRDDGL+LAAGTSNGRVVFYDVRGKPEP
Sbjct: 241 SVGLDKKLYTYDSGSRRPSSFVAYEAPFSSMAFRDDGLILAAGTSNGRVVFYDVRGKPEP 300

Query: 301 YIVLRAYSSSEAVTSLSWQRLKPVIVNEGNCTAEVALLGGAVEDSILMPDPLPSVTTSTV 360
           YIVLRAY+SSEAVTSLSWQRLKPVIVNEGNCTAEVALLGGA+EDSILMPDPLPSVTTSTV
Sbjct: 301 YIVLRAYTSSEAVTSLSWQRLKPVIVNEGNCTAEVALLGGAIEDSILMPDPLPSVTTSTV 360

Query: 361 ALSTTVSSSRNPGRSGPTFEASLTETSSTFSTAEETPHRSHLRPGG--MLARLHAPRSSY 420
            LSTT+SSSRNPGRSGPTFEASLTETSSTFSTAEETP RSHLRPGG   LARLH PRSSY
Sbjct: 361 PLSTTISSSRNPGRSGPTFEASLTETSSTFSTAEETPLRSHLRPGGSLALARLHPPRSSY 420

Query: 421 NFKDDMEVFSPLVDVQPITPSLDKLWDEQNGQKKDHPFDKKPLSLLFPSSSRRFSSIEDG 480
           NFKDDMEVFSPLVDVQPITPSLDKLWD+ NG KKD+PFDKKPLS+LFPSSSRRFSSIEDG
Sbjct: 421 NFKDDMEVFSPLVDVQPITPSLDKLWDDPNGPKKDNPFDKKPLSMLFPSSSRRFSSIEDG 480

Query: 481 ASDHPIFNWKSSSSRQDDSRSSSGQLGSTPAPTLNSNNEDSSITPPEAWGGEKLSDKFAQ 540
           ASDHPI++WKSSSS+QDDSRSSSGQLGSTPAP LNS NEDSSITPPEAWGGEKLS+KFAQ
Sbjct: 481 ASDHPIYHWKSSSSKQDDSRSSSGQLGSTPAPALNSKNEDSSITPPEAWGGEKLSEKFAQ 540

Query: 541 LRQPVSLPSRFGMMASGSSSQTSSSMISGLQDPSSSVSQSSITSLTNLNFSYPNLRTKDV 600
           LRQP++LPSRFGM+AS SSSQTSSSMISGLQDPSSSVSQSSITSLTNLNFSYPNLR KDV
Sbjct: 541 LRQPMTLPSRFGMLASSSSSQTSSSMISGLQDPSSSVSQSSITSLTNLNFSYPNLRIKDV 600

Query: 601 TSQEVSLSIPEHFSTTAASLSLGTRGTIGLSNQDSPRNTTMTLPRRFSTYAERLSTTSSF 660
           TSQ+VSLSIPEH S+TA+SLSLGTR  IGLSN DSPR T MTLPRRFSTYAERLSTTSSF
Sbjct: 601 TSQDVSLSIPEHLSSTASSLSLGTRVNIGLSNTDSPRATAMTLPRRFSTYAERLSTTSSF 660

Query: 661 SDGLPMGSPKTKKMGSETREEVLNNLSKFDTLSVTESGILPAINVGSLQPQKALQSDAQQ 720
           SDGLPMGSPKTKKMGSETREEVLNNLSKFDTLSVTE+GI P++N GSLQPQKALQSDAQQ
Sbjct: 661 SDGLPMGSPKTKKMGSETREEVLNNLSKFDTLSVTEAGIPPSMNGGSLQPQKALQSDAQQ 720

Query: 721 GNSFTLQLFQRTLEETLDSFQRSIHDDMRNLHLEILRQFHMQEMEMSSVMSTILENQAEL 780
           GNSFTLQLFQRTLEETLDSFQRSIHDDMRNLHLEILRQFHMQEMEMSSVMS+ILENQAEL
Sbjct: 721 GNSFTLQLFQRTLEETLDSFQRSIHDDMRNLHLEILRQFHMQEMEMSSVMSSILENQAEL 780

Query: 781 IKEVKSLRKENQQLRDLLGS 799
           IKEVKSLRKENQQLRDLLGS
Sbjct: 781 IKEVKSLRKENQQLRDLLGS 800

BLAST of Lag0030312 vs. NCBI nr
Match: XP_023550844.1 (protein NEDD1-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023550845.1 protein NEDD1-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1462.2 bits (3784), Expect = 0.0e+00
Identity = 749/800 (93.62%), Postives = 777/800 (97.12%), Query Frame = 0

Query: 1   MNSGDQSMALLAASAGDTVKLFDVSVHSNDLGDPCTLSYTPSPGYQVNSVKWNHTNLVVA 60
           MNSGD SMALLAASAGDTVKLFDVSVHSNDLGDPCTLSYTPSPGYQVNSVKWNHTNLVVA
Sbjct: 1   MNSGDPSMALLAASAGDTVKLFDVSVHSNDLGDPCTLSYTPSPGYQVNSVKWNHTNLVVA 60

Query: 61  SAGDDKKISLWRKNGQSMGTIPIAGSDSGDNIEESIFSISFSNKVSRYMCTGGSGHVVRI 120
           SAGDDKKISLWRKNGQSMGTIPIAG+DSGDNIEESIFSISFS+KVSRYMCTGGSGHVVRI
Sbjct: 61  SAGDDKKISLWRKNGQSMGTIPIAGNDSGDNIEESIFSISFSSKVSRYMCTGGSGHVVRI 120

Query: 121 WDLQRKRCIKWLRGHTNTITGAMYNCKDEHLASISLSGDLILHNLASGARAAELKDPNEQ 180
           WDLQRKRCIKWLRGHTNTITGAMYNCKDEHLASISLSGDLILHNLASGARA ELKDPNEQ
Sbjct: 121 WDLQRKRCIKWLRGHTNTITGAMYNCKDEHLASISLSGDLILHNLASGARATELKDPNEQ 180

Query: 181 VLRVLDYSRISRHLLVTAGDDGTVHLWDTTGRNPKVSWQKQHSAPTAGIGFSPSNDKTLA 240
           VLRVLDYSRISRHLLVTAGDDGTVHLWDTTGRNPKVSWQKQHSAPTAGIGFSPSNDKTLA
Sbjct: 181 VLRVLDYSRISRHLLVTAGDDGTVHLWDTTGRNPKVSWQKQHSAPTAGIGFSPSNDKTLA 240

Query: 241 SVGLDKKLYTYDSGSRRPSSFIAYEAPFSSMAFRDDGLVLAAGTSNGRVVFYDVRGKPEP 300
           SVG+DKKLYTYDSGSRRPSSFIAYEAPFSSMAFRDDGL+LAAGTSNGRVVFYDVRGKPEP
Sbjct: 241 SVGMDKKLYTYDSGSRRPSSFIAYEAPFSSMAFRDDGLILAAGTSNGRVVFYDVRGKPEP 300

Query: 301 YIVLRAYSSSEAVTSLSWQRLKPVIVNEGNCTAEVALLGGAVEDSILMPDPLPSVTTSTV 360
           YIVLRA+SSSEAVTSLSWQRLKPVIVNEGNCTAEVALLGGA+EDSILMPDPLPSVTTST+
Sbjct: 301 YIVLRAFSSSEAVTSLSWQRLKPVIVNEGNCTAEVALLGGAIEDSILMPDPLPSVTTSTI 360

Query: 361 ALSTTVSSSRNPGRSGPTFEASLTETSSTFSTAEETPHRSHLRPGGMLARLHAPRSSYNF 420
            +STT+SSSRNPGRSGPTFEASL ETSSTF+TAEETPHRSHL P G LARLHAPRSSYNF
Sbjct: 361 PMSTTISSSRNPGRSGPTFEASLAETSSTFTTAEETPHRSHLWPLGSLARLHAPRSSYNF 420

Query: 421 KDDMEVFSPLVDVQPITPSLDKLWDEQNGQKKDHPFDKKPLSLLFPSSSRRFSSIEDGAS 480
           KDDMEVFSPLVDVQPITPSLDKLWD+QNG KKDHPFDKKPLS+LFPSS+RRFSSIEDGA 
Sbjct: 421 KDDMEVFSPLVDVQPITPSLDKLWDDQNGLKKDHPFDKKPLSMLFPSSNRRFSSIEDGAG 480

Query: 481 DHPIFNWKSSSSRQDDSRSSSGQLGSTPAPTLNSNNEDSSITPPEAWGGEKLSDKFAQLR 540
           DHPIF+WKSSSS+QDDSRSSSG LGS+P  TLNS +EDSSITPPEAWGGEKLSDKFAQLR
Sbjct: 481 DHPIFHWKSSSSKQDDSRSSSGPLGSSPTSTLNSKSEDSSITPPEAWGGEKLSDKFAQLR 540

Query: 541 QPVSLPSRFGMMA--SGSSSQTSSSMISGLQDPSSSVSQSSITSLTNLNFSYPNLRTKDV 600
           QP++LPSRFGM+A  S SSSQTSSS+ISGLQDPSSSVSQSSITSLTNLNFSYPNLRTK+V
Sbjct: 541 QPMTLPSRFGMLASSSSSSSQTSSSLISGLQDPSSSVSQSSITSLTNLNFSYPNLRTKEV 600

Query: 601 TSQEVSLSIPEHFSTTAASLSLGTRGTIGLSNQDSPRNTTMTLPRRFSTYAERLSTTSSF 660
           TSQEVSLSIPEH STTAASLSLGTRGT+GLSN DSP NTTMTLPRRFSTYAERLSTTSSF
Sbjct: 601 TSQEVSLSIPEHLSTTAASLSLGTRGTVGLSNPDSPWNTTMTLPRRFSTYAERLSTTSSF 660

Query: 661 SDGLPMGSPKTKKMGSETREEVLNNLSKFDTLSVTESGILPAINVGSLQPQKALQSDAQQ 720
           SDGLP+GSPKTKKMGSETREEVLNNLSKFDTLSV ESGILPA+N GSLQPQKALQ DAQQ
Sbjct: 661 SDGLPVGSPKTKKMGSETREEVLNNLSKFDTLSVAESGILPAMNGGSLQPQKALQPDAQQ 720

Query: 721 GNSFTLQLFQRTLEETLDSFQRSIHDDMRNLHLEILRQFHMQEMEMSSVMSTILENQAEL 780
           GNSFTLQLFQRTLEETLDSFQRSIHDDMRNLH+EILRQFHMQE+EMSSV S+ILENQAEL
Sbjct: 721 GNSFTLQLFQRTLEETLDSFQRSIHDDMRNLHIEILRQFHMQEIEMSSVTSSILENQAEL 780

Query: 781 IKEVKSLRKENQQLRDLLGS 799
           IKEVKSLRKENQQLRDLLGS
Sbjct: 781 IKEVKSLRKENQQLRDLLGS 800

BLAST of Lag0030312 vs. NCBI nr
Match: KAG6602643.1 (Protein NEDD1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1460.3 bits (3779), Expect = 0.0e+00
Identity = 750/800 (93.75%), Postives = 773/800 (96.62%), Query Frame = 0

Query: 1   MNSGDQSMALLAASAGDTVKLFDVSVHSNDLGDPCTLSYTPSPGYQVNSVKWNHTNLVVA 60
           M SGD SMALLAASAGDTVKLFDVS +SNDLGDPCTLSYTPSPGYQVNSVKWNHTNLVVA
Sbjct: 1   MTSGDPSMALLAASAGDTVKLFDVSAYSNDLGDPCTLSYTPSPGYQVNSVKWNHTNLVVA 60

Query: 61  SAGDDKKISLWRKNGQSMGTIPIAGSDSGDNIEESIFSISFSNKVSRYMCTGGSGHVVRI 120
           SAGDDK+ISLWRKNGQSMGTIPIAGSD GDNIEESIFSISFSNKVSRYMCTGGSGHVVRI
Sbjct: 61  SAGDDKRISLWRKNGQSMGTIPIAGSDGGDNIEESIFSISFSNKVSRYMCTGGSGHVVRI 120

Query: 121 WDLQRKRCIKWLRGHTNTITGAMYNCKDEHLASISLSGDLILHNLASGARAAELKDPNEQ 180
           WDLQRKRCIKWLRGHTNTITGAMYNCKDEHLASISLSGDL+LHNLASGARAAELKDPNEQ
Sbjct: 121 WDLQRKRCIKWLRGHTNTITGAMYNCKDEHLASISLSGDLLLHNLASGARAAELKDPNEQ 180

Query: 181 VLRVLDYSRISRHLLVTAGDDGTVHLWDTTGRNPKVSWQKQHSAPTAGIGFSPSNDKTLA 240
           VLRVLDYSRISRHLLVTAGD+GTVHLWDTTGRNPKVSWQKQHSAPTAGI FSPSNDKTLA
Sbjct: 181 VLRVLDYSRISRHLLVTAGDNGTVHLWDTTGRNPKVSWQKQHSAPTAGISFSPSNDKTLA 240

Query: 241 SVGLDKKLYTYDSGSRRPSSFIAYEAPFSSMAFRDDGLVLAAGTSNGRVVFYDVRGKPEP 300
           SVGLDKKLYTYDSGSRRPSSFIAYEAPFSSMAFRDDGL+LAAGTSNGRVVFYDVRGKPEP
Sbjct: 241 SVGLDKKLYTYDSGSRRPSSFIAYEAPFSSMAFRDDGLILAAGTSNGRVVFYDVRGKPEP 300

Query: 301 YIVLRAYSSSEAVTSLSWQRLKPVIVNEGNCTAEVALLGGAVEDSILMPDPLPSVTTSTV 360
           YIVLRAYSS EAVTS+SWQRLKPVIVNEGNCTA+VALLGGA+EDSILMPDPLPSVTTSTV
Sbjct: 301 YIVLRAYSSLEAVTSISWQRLKPVIVNEGNCTADVALLGGAIEDSILMPDPLPSVTTSTV 360

Query: 361 ALSTTVSSSRNPGRSGPTFEASLTETSSTFSTAEETPHRSHLRPGGMLARLHAPRSSYNF 420
           ALSTT SSSRNPG SG TFEASLTETSST STAEETPHRSHLRPGG L RLHAPRS+YNF
Sbjct: 361 ALSTTTSSSRNPGHSGLTFEASLTETSSTISTAEETPHRSHLRPGGSLTRLHAPRSNYNF 420

Query: 421 KDDMEVFSPLVDVQPITPSLDKLWDEQNGQKKDHPFDKKPLSLLFPSSSRRFSSIEDGAS 480
           KDDMEVFSPLVDVQPITPSLDKLWD+QNG KKDHPFDKKPL LLFPSSSRRFSSIEDG S
Sbjct: 421 KDDMEVFSPLVDVQPITPSLDKLWDDQNGLKKDHPFDKKPLPLLFPSSSRRFSSIEDGPS 480

Query: 481 DHPIFNWKSSSSRQDDSRSSSGQLGSTPAPTLNSNNEDSSITPPEAWGGEKLSDKFAQLR 540
           DHPIFNWKSSSS+QDDSRSSSGQLGSTPAPTLNS NEDSSITPPEAWGGEKLSDKFAQLR
Sbjct: 481 DHPIFNWKSSSSKQDDSRSSSGQLGSTPAPTLNSKNEDSSITPPEAWGGEKLSDKFAQLR 540

Query: 541 QPVSLPSRFGMMASGSSSQTSSSMISGLQDPSSSVSQSSITSLTNLNFSYPNLRTKDVTS 600
           QP +LPSRFGM+AS SSSQTSSSMISGLQDPSSS++QSSITSLTNLNFSYPN+RTKDV S
Sbjct: 541 QPATLPSRFGMLASSSSSQTSSSMISGLQDPSSSITQSSITSLTNLNFSYPNMRTKDVIS 600

Query: 601 QEVSLSIPEHFSTTAASLSLGTRGTIGLSNQDSPRNTTMTLPRRFSTYAERLSTTSSFSD 660
           QEVSLSIPEHFSTTAASLSLGTRGTIGLSNQDSPR TTMTLPRRFSTYAERLSTTSSFSD
Sbjct: 601 QEVSLSIPEHFSTTAASLSLGTRGTIGLSNQDSPRTTTMTLPRRFSTYAERLSTTSSFSD 660

Query: 661 GLP--MGSPKTKKMGSETREEVLNNLSKFDTLSVTESGILPAINVGSLQPQKALQSDAQQ 720
           GLP  +GSPKTKKMGSETREEVLNNLSKFDTLSVTESGILPA+N G L  QKALQ+DAQQ
Sbjct: 661 GLPVGVGSPKTKKMGSETREEVLNNLSKFDTLSVTESGILPAMNGGPLLQQKALQTDAQQ 720

Query: 721 GNSFTLQLFQRTLEETLDSFQRSIHDDMRNLHLEILRQFHMQEMEMSSVMSTILENQAEL 780
           GNSFTLQLFQRTLEETLDSFQRSIHDDMRNLHLEILRQFHMQEMEMSSV S++L+NQAEL
Sbjct: 721 GNSFTLQLFQRTLEETLDSFQRSIHDDMRNLHLEILRQFHMQEMEMSSVTSSMLKNQAEL 780

Query: 781 IKEVKSLRKENQQLRDLLGS 799
           IKEVKSLRKENQQLR+LLGS
Sbjct: 781 IKEVKSLRKENQQLRNLLGS 800

BLAST of Lag0030312 vs. ExPASy Swiss-Prot
Match: B3H5K9 (Protein NEDD1 OS=Arabidopsis thaliana OX=3702 GN=NEDD1 PE=2 SV=1)

HSP 1 Score: 869.0 bits (2244), Expect = 4.1e-251
Identity = 483/813 (59.41%), Postives = 593/813 (72.94%), Query Frame = 0

Query: 2   NSGDQSMALLAASAGDTVKLFDVSVHSNDLGDPCTLSYTPSPGYQVNSVKWNHTNLVVAS 61
           N  + S  LLAAS GDTVKLFDVS    D GDPC LSYTPSPG  VNSVKWNHTNLVVAS
Sbjct: 4   NLVEPSWRLLAASGGDTVKLFDVSA---DSGDPCVLSYTPSPGCAVNSVKWNHTNLVVAS 63

Query: 62  AGDDKKISLWRKNGQSMGTIPIAGSDSGDNIEESIFSISFSNKVSRYMCTGGSGHVVRIW 121
            G+DKKISLWRKNGQS+GT+P+ G D GD+ EE + +ISFS K SRY+C+GG+G +V+IW
Sbjct: 64  TGEDKKISLWRKNGQSLGTVPVTGKDGGDSAEECLSAISFSKKGSRYICSGGTGQIVKIW 123

Query: 122 DLQRKRCIKWLRGHTNTITGAMYNCKDEHLASISLSGDLILHNLASGARAAELKDPNEQV 181
           DLQRK CIK L+GHT+TITG MYNCKDEHLAS+S+ GDLI+HNLASGARA ELKDPN QV
Sbjct: 124 DLQRKLCIKKLKGHTSTITGVMYNCKDEHLASVSVGGDLIVHNLASGARATELKDPNGQV 183

Query: 182 LRVLDYSRISRHLLVTAGDDGTVHLWDTTGRNPKVSWQKQHSAPTAGIGFSPSNDKTLAS 241
           LR+LDYSR SRHLLVTAGDDGTVHLWDTTGR+PK+SW KQHSAPTAG+ FSPSN+K +AS
Sbjct: 184 LRLLDYSRSSRHLLVTAGDDGTVHLWDTTGRSPKMSWLKQHSAPTAGVCFSPSNEKIIAS 243

Query: 242 VGLDKKLYTYDSGSRRPSSFIAYEAPFSSMAFRDDGLVLAAGTSNGRVVFYDVRGKPEPY 301
           VG+DKKLYTYDSGSRR SS IAYEAPFSS+AF D+G +L AGTSNGRVVFYD+RGKP+P 
Sbjct: 244 VGMDKKLYTYDSGSRRSSSCIAYEAPFSSLAFGDNGYILVAGTSNGRVVFYDIRGKPQPV 303

Query: 302 IVLRAYSSSEAVTSLSWQRLKPVIVNEGNCTAEVALLGGAVEDSILMPDPLPSVTTSTVA 361
            VL A+S+SE VTSLSWQ  KPVIVNE N T+E+ALLG  VEDS+++PDPLPS T S   
Sbjct: 304 TVLHAFSNSEDVTSLSWQTSKPVIVNEKNYTSEMALLGSTVEDSVVIPDPLPSTTPS--- 363

Query: 362 LSTTVSSSRNPGRSGPTFEASLTETSSTFSTAEETPHRSHLRPGGMLARLHAPRSSYNFK 421
                 S+  PG  G      ++ ++   S+ E+TP+R+HL P G L RLHA R++ ++ 
Sbjct: 364 ---ASQSAMAPGSRG------VSASTVNASSVEQTPNRNHLWPSGPLGRLHALRANDSYN 423

Query: 422 DDMEVFSPLVDVQPITPSLDKLWDEQNGQKKDH-PFDKKPLSLLFPSSSRRFSSIEDGAS 481
           DDM VFSP++DV     S++K  D +    KDH   D KP SLLFPSSS+ +S  ++G+ 
Sbjct: 424 DDMGVFSPIIDVS----SVEKWADSEGYNNKDHLVVDNKPSSLLFPSSSKGYSFGDNGSK 483

Query: 482 DHPIFNWK-SSSSRQDDSRSSSGQLGSTPAPTLNSNNEDSSITPPEAWGGEKLSDKFAQL 541
           +HPIF+WK SS+S+QDD R++    GS   PT +S +EDS++TPPEAWGG+K S+KF QL
Sbjct: 484 EHPIFDWKPSSTSKQDDPRAAFSSFGSI-TPTASSKSEDSALTPPEAWGGDKFSEKFNQL 543

Query: 542 RQP--------VSLPSRFGMMASGSSSQTSSSMISGLQDPSSSVSQSSITSLTNLNFSYP 601
                      +  PSR  + ++G+S  TS SM S  +D   S  Q   T+  N +  +P
Sbjct: 544 ANEKFSDKFSHLHAPSRLAVSSTGAS--TSGSMFSSSRDFPLSHGQ---TNFANASLEFP 603

Query: 602 NLR----TKDVTSQEVSLSIPEHFSTTAASLSLGTRGTIGLSNQDSPRNTTMTLPRRFST 661
            +R    T + +S +   ++P        S  L T+G     N DS R  +    RRFST
Sbjct: 604 RIRDFSSTFETSSTQTDNNLP--------SSPLFTKGITAPGNIDSLR-LSPNFTRRFST 663

Query: 662 YAERLSTTSSFSDGLPM---GSPKTKKMGSETREEVLNN-LSKFDTLSVTESGILPAINV 721
           YAER+STTSSFSDG  +   GSPK KK GSETREEVLN+ L++ +T+  TE+G +P +N 
Sbjct: 664 YAERISTTSSFSDGASLSLGGSPKIKKTGSETREEVLNHLLARPETVVATEAGAMPLMNQ 723

Query: 722 GSLQPQKALQSDAQQGNSFTLQLFQRTLEETLDSFQRSIHDDMRNLHLEILRQFHMQEME 781
           G L+  +  Q      ++FTLQLFQRTLE TLDSFQ SIHDD+RNLH+EILRQFHM EME
Sbjct: 724 GGLKQSQTDQQQVMGSSNFTLQLFQRTLEGTLDSFQNSIHDDVRNLHIEILRQFHMHEME 782

Query: 782 MSSVMSTILENQAELIKEVKSLRKENQQLRDLL 797
           MS V+S+ILENQAE +KE+K LRKENQ+LR  L
Sbjct: 784 MSKVLSSILENQAEQMKELKLLRKENQELRQRL 782

BLAST of Lag0030312 vs. ExPASy Swiss-Prot
Match: Q3B7M6 (Protein NEDD1 OS=Bos taurus OX=9913 GN=NEDD1 PE=2 SV=1)

HSP 1 Score: 176.0 bits (445), Expect = 1.7e-42
Identity = 196/803 (24.41%), Postives = 336/803 (41.84%), Query Frame = 0

Query: 13  ASAGDTVKLFDVSVHSNDLGDPCTLSYTPSPGYQVNSVKWNHTN--LVVASAGDDKKISL 72
           AS+GD VK++D S  S  L D       P   + ++SV W+  N  LV AS+  DK +  
Sbjct: 8   ASSGDDVKIWDAS--SMTLVDKFNPHTAP---HAISSVCWSSNNNFLVTASSSGDKIVV- 67

Query: 73  WRKNGQSMGTIPIAGSDSGDNIEESIFSISFSNKVSRYMCTGGSGHVVRIWDLQRKRCIK 132
                 S    P+   + G+  +++  S+   N  S Y+ +GG  + V IWDL+ KR  +
Sbjct: 68  -----SSCKCKPVPLLELGEGQKQTCVSL---NSTSMYLVSGGLNNTVNIWDLKSKRVHR 127

Query: 133 WLRGHTNTITGAMYNCKDEHLASISLSGDLILHNLASGARAAELKDPNEQVLRVLDYSRI 192
            L+ H + +T   YN  D ++AS SLSG++ILH++ +   +      + Q +R L YS  
Sbjct: 128 SLKDHKDEVTCVTYNWNDCYIASGSLSGEIILHSVTTNLSSTPFGHGSNQSIRHLKYSLF 187

Query: 193 SRHLLVTAGDDGTVHLWDTTGRNPKVSWQKQHSAPTAGIGFSPSNDKTLASVGLDKKLYT 252
            + LL +  D+G V LWD   ++P  ++   H AP +GI FSP N+    +VGLDK++  
Sbjct: 188 KKSLLGSVSDNGIVTLWDVNSQSPYHNFDSTHKAPASGICFSPVNELLFVTVGLDKRIIL 247

Query: 253 YDSGSRRPSSFIAYEAPFSSMAFRDDGLVLAAGTSNGRVVFYDVRGKPEPYIVLRAYSSS 312
           YD+ S++    +  +AP +++ F  DG  LA G+S G++  YD+R    P   + A+ +S
Sbjct: 248 YDTSSKKLVKTLVADAPLTAVDFMPDGATLAIGSSRGKIYQYDLRMLKSPIKTISAHKTS 307

Query: 313 EAVTSLSWQRL------------KPVIVNEGNCTAEVALLGGAVEDSILMPDPLPSVTTS 372
               +  +  +            KP  VN+   TA V+  GG  ++    P  +    T+
Sbjct: 308 VQCIAFQYSTVLSKSGLNKGCSNKPTAVNKR--TANVSAGGGGAQN----PGVVREAATT 367

Query: 373 TVAL--STTVSSSRNPGRSGPTFEASLTETSSTFSTAEETPHRSHLRPGGMLARLHAPRS 432
           ++A       +++   G   P  +A L  + +T + ++E             A     + 
Sbjct: 368 SIATVPPQPTAAAVGKGAVAPQDKAGLPRSINTDTLSKE-------------AESGKNQD 427

Query: 433 SYNFKDD-----MEVFSPLVDVQPITPSLDKLWDEQNGQKKDHPFDKKP-LSLLFPSSSR 492
             NF D       ++FSP+ D   ++    K  DE  G  K    D  P L+ +FP    
Sbjct: 428 FSNFDDSGKSSLGDMFSPVRDDAVVS----KGGDESIG--KGDGLDFLPQLNSVFPPRKN 487

Query: 493 RFSSIEDGASDHPIFNWKSSSSRQDDSRSSSGQLGSTPAPTLNSNNEDSSITPPEAWGGE 552
              S        P+  +  S  +++                 N N++ ++ +     G +
Sbjct: 488 PVVSSTSVLHSSPLNVFMGSPGKEE-----------------NENHDLTAESKKMYLGKQ 547

Query: 553 KLSDKFAQLRQPVSLPSRFGMMASGSSSQTSSSMISGLQDPSSSVSQSSITSLTNLNFSY 612
           +  D F Q  + +S      + AS SS+QT S      + P   +    I        S 
Sbjct: 548 ESKDSFKQFAKLISGAETGNLNASPSSNQTRSP--EKFEKPEKEIEAQLINEPPGNGSST 607

Query: 613 PNLRTKDVTSQEVSLSIPEHFSTTAASLSLGTRGTIGLSNQDSPRNTTMTLPRRFSTYAE 672
           PN +                    A+S++ G  G++                      +E
Sbjct: 608 PNPK-------------------IASSVTAGVAGSL----------------------SE 656

Query: 673 RLSTTSSFSDGLPMGSPKTKKMGSETREEVLNNLSKFDTLSVTESGILPAINVGSLQPQK 732
           ++  T                                               +G+ +P  
Sbjct: 668 KIVDT-----------------------------------------------IGNSRPNA 656

Query: 733 ALQSDAQQGNSFTLQLFQRTLEETLDSFQRSIHDDMRNLHLEILRQFHMQEMEMSSVMST 792
            L       +S  ++  Q  ++ETLD F+ + H D+ NL +E+++QFHMQ  EM S++  
Sbjct: 728 PL-------SSVQIRFIQNMIQETLDDFREACHRDIVNLQVEMIKQFHMQLNEMHSLLER 656

Query: 793 ILENQAELIKEVKSLRKENQQLR 794
              N+  L+ E++ LR+EN++LR
Sbjct: 788 YSVNEG-LVAEIERLREENKRLR 656

BLAST of Lag0030312 vs. ExPASy Swiss-Prot
Match: Q8NHV4 (Protein NEDD1 OS=Homo sapiens OX=9606 GN=NEDD1 PE=1 SV=1)

HSP 1 Score: 173.3 bits (438), Expect = 1.1e-41
Identity = 186/803 (23.16%), Postives = 332/803 (41.34%), Query Frame = 0

Query: 13  ASAGDTVKLFDVSVHSNDLGDPCTLSYTPSPGYQVNSVKWNHTN--LVVASAGDDKKISL 72
           AS+GD +K++D S  S  L D      +P   + ++S+ W+  N  LV AS+  DK +  
Sbjct: 8   ASSGDDIKIWDAS--SMTLVDKFNPHTSP---HGISSICWSSNNNFLVTASSSGDKIVV- 67

Query: 73  WRKNGQSMGTIPIAGSDSGDNIEESIFSISFSNKVSRYMCTGGSGHVVRIWDLQRKRCIK 132
                 S    P+   +  +  +++  ++   N  S Y+ +GG  + V IWDL+ KR  +
Sbjct: 68  -----SSCKCKPVPLLELAEGQKQTCVNL---NSTSMYLVSGGLNNTVNIWDLKSKRVHR 127

Query: 133 WLRGHTNTITGAMYNCKDEHLASISLSGDLILHNLASGARAAELKDPNEQVLRVLDYSRI 192
            L+ H + +T   YN  D ++AS SLSG++ILH++ +   +      + Q +R L YS  
Sbjct: 128 SLKDHKDQVTCVTYNWNDCYIASGSLSGEIILHSVTTNLSSTPFGHGSNQSVRHLKYSLF 187

Query: 193 SRHLLVTAGDDGTVHLWDTTGRNPKVSWQKQHSAPTAGIGFSPSNDKTLASVGLDKKLYT 252
            + LL +  D+G V LWD   ++P  ++   H AP +GI FSP N+    ++GLDK++  
Sbjct: 188 KKSLLGSVSDNGIVTLWDVNSQSPYHNFDSVHKAPASGICFSPVNELLFVTIGLDKRIIL 247

Query: 253 YDSGSRRPSSFIAYEAPFSSMAFRDDGLVLAAGTSNGRVVFYDVRGKPEPYIVLRAYSSS 312
           YD+ S++    +  + P +++ F  DG  LA G+S G++  YD+R    P   + A+ +S
Sbjct: 248 YDTSSKKLVKTLVADTPLTAVDFMPDGATLAIGSSRGKIYQYDLRMLKSPVKTISAHKTS 307

Query: 313 EAVTSLSWQRL------------KPVIVNEGNCTAEVALLGGAVEDSILMPDPLPSVTTS 372
               +  +  +            KP  VN+ +     A  GG     I+   P  S+ T 
Sbjct: 308 VQCIAFQYSTVLTKSSLNKGCSNKPTTVNKRSVNVNAA-SGGVQNSGIVREAPATSIAT- 367

Query: 373 TVALSTTVSSSRNPGRSGPTFEASLTETSSTFSTAEETPHRSHLRPGGMLARLHAPRSSY 432
              L   ++S+   G      +A L  + +T + ++ET                     +
Sbjct: 368 --VLPQPMTSAMGKGTVAVQEKAGLPRSINTDTLSKETD--------------SGKNQDF 427

Query: 433 NFKDDM------EVFSPLVDVQPITPSLDKLWDEQNGQKKDHPFDKKP-LSLLFPSSSRR 492
           +  DD       ++FSP+ D   +    D+   + +G      FD  P L+ +FP     
Sbjct: 428 SSFDDTGKSSLGDMFSPIRDDAVVNKGSDESIGKGDG------FDFLPQLNSVFPPRKNP 487

Query: 493 FSSIEDGASDHPIFNWKSSSSRQDDSRSSSGQLGSTPAPTLNSNNEDSSITPPEAWGGEK 552
            +S        P+  +  S  +++                 N N + ++ +     G ++
Sbjct: 488 VTSSTSVLHSSPLNVFMGSPGKEE-----------------NENRDLTAESKKIYMGKQE 547

Query: 553 LSDKFAQLRQPVSLPSRFG-MMASGSSSQTSSSMISGLQDPSSSVSQSSITSLTNLNFSY 612
             D F QL + V+  +  G +  S SS+QT +S     + P + +    I          
Sbjct: 548 SKDSFKQLAKLVTSGAESGNLNTSPSSNQTRNS--EKFEKPENEIEAQLICE-------- 607

Query: 613 PNLRTKDVTSQEVSLSIPEHFSTTAASLSLGTRGTIGLSNQDSPRNTTMTLPRRFSTYAE 672
           P +      + +++ S+    +  A+SLS     +IG + Q++P                
Sbjct: 608 PPINGSSTPNPKIASSVT---AGVASSLSEKIADSIGNNRQNAPL--------------- 657

Query: 673 RLSTTSSFSDGLPMGSPKTKKMGSETREEVLNNLSKFDTLSVTESGILPAINVGSLQPQK 732
                                                                       
Sbjct: 668 ------------------------------------------------------------ 657

Query: 733 ALQSDAQQGNSFTLQLFQRTLEETLDSFQRSIHDDMRNLHLEILRQFHMQEMEMSSVMST 792
                     S  ++  Q  ++ETLD F+ + H D+ NL +E+++QFHMQ  EM S++  
Sbjct: 728 ---------TSIQIRFIQNMIQETLDDFREACHRDIVNLQVEMIKQFHMQLNEMHSLLER 657

Query: 793 ILENQAELIKEVKSLRKENQQLR 794
              N+  L+ E++ LR+EN++LR
Sbjct: 788 YSVNEG-LVAEIERLREENKRLR 657

BLAST of Lag0030312 vs. ExPASy Swiss-Prot
Match: P33215 (Protein NEDD1 OS=Mus musculus OX=10090 GN=Nedd1 PE=1 SV=2)

HSP 1 Score: 166.4 bits (420), Expect = 1.3e-39
Identity = 195/796 (24.50%), Postives = 339/796 (42.59%), Query Frame = 0

Query: 13  ASAGDTVKLFDVS-VHSNDLGDPCTLSYTPSPGYQVNSVKWNHTN--LVVASAGDDKKIS 72
           AS+GD VK++D S +   D  +P T     SP + ++S+ W+  N  LV AS+  DK + 
Sbjct: 8   ASSGDDVKIWDASFLTLVDKFNPHT-----SP-HGISSICWSSNNNFLVTASSSGDKIVV 67

Query: 73  LWRKNGQSMGTIPIAGSDSGDNIEESIFSISFSNKVSRYMCTGGSGHVVRIWDLQRKRCI 132
                  S    P+   +  +  +++   +   N  S Y+ +GG  + V IWDL+ KR  
Sbjct: 68  ------SSCKCKPVPLLELAEGQKQTCVDL---NSTSMYLASGGLNNTVNIWDLKSKRLH 127

Query: 133 KWLRGHTNTITGAMYNCKDEHLASISLSGDLILHNLASGARAAELKDPNEQVLRVLDYSR 192
           + L+ H   +T   YN  D ++AS SLSG++ILH++ +   +      ++Q +R + YS 
Sbjct: 128 RSLKDHKCEVTCVAYNWNDCYIASGSLSGEIILHSVTTNTSSTPFGHGSKQPIRHIKYSL 187

Query: 193 ISRHLLVTAGDDGTVHLWDTTGRNPKVSWQKQHSAPTAGIGFSPSNDKTLASVGLDKKLY 252
             + LL +  D+G V LWD   ++   ++   H AP +GI FSP N+    ++GLDK++ 
Sbjct: 188 FRKSLLGSVSDNGVVTLWDVNSQSSYHTFDSTHKAPASGICFSPVNELLFVTIGLDKRII 247

Query: 253 TYDSGSRRPSSFIAYEAPFSSMAFRDDGLVLAAGTSNGRVVFYDVRGKPEPYIVLRAYSS 312
            YD+ S++    +  + P +++ F  DG  LA G+S G++  YD+R    P   + A+ +
Sbjct: 248 LYDTSSKKLVKTLVADTPLTAVDFMPDGATLAIGSSRGKIYQYDLRMLKSPVKTISAHKT 307

Query: 313 S------EAVTSLSWQRLKPVIVNEGNC----TAEVALLGGAVEDS-ILMPDPLPSVTTS 372
           S      +  TSL+   L     N+       +  V+   GA ++S I+   P PS+ T 
Sbjct: 308 SVQCIAFQYSTSLTKASLSKGSSNKATAVNKRSVPVSSSSGAAQNSGIVREAPSPSIAT- 367

Query: 373 TVALSTTVSSSRNPGRSGPTFEASLTETSSTFSTAEETPHRSHLRPGGMLARLHAPRSSY 432
              L   V+++   G      EA L  + ST   ++ET        G          +  
Sbjct: 368 --VLPQPVTTALGKGSGAAQDEAGLARSKSTDIFSKETD------AGKSQDFSSFDDTGK 427

Query: 433 NFKDDMEVFSPLVDVQPITPSLDKLWDEQNGQKKDHPFDKKP-LSLLFPSSSRRFSSIED 492
           N   DM  FSP+ D   ++    K  DE  G  K    D  P L+ +FP      +S   
Sbjct: 428 NSLGDM--FSPIRDDAVVS----KGGDESIG--KGDGLDFLPQLNSVFPLRKNAGASSSL 487

Query: 493 GASDHPIFNWKSSSSRQDDSRSSSGQLGSTPAPTLNSNNEDSSITPPEAWGGEKLSDKFA 552
                P+     SS +++                 N ++E S+ +     G ++  D   
Sbjct: 488 VLHSSPLNVLMGSSGKEE-----------------NESHEPSAESKRAYLGKQEPKDAMK 547

Query: 553 QLRQPVSLPSRFGMMASGSSSQTSSSMISGLQDPSSSVSQSSITSLTNLNFSYPNLRTKD 612
           Q  + +S  +  G++ +  SS  + ++                       F  P    KD
Sbjct: 548 QFTKLISSGAEPGILNTCPSSNQARNL---------------------EKFEKPE---KD 607

Query: 613 VTSQEVSLSIPEHFSTTAASLSLGTRGTIGLSNQDSPRNTTMTLPRRFSTYAERLSTTSS 672
           + +Q                          L ++ S   ++ T+P+  S+          
Sbjct: 608 IEAQ--------------------------LIHEPSVNGSSTTVPKAASSV--------- 657

Query: 673 FSDGLPMGSPKTKKMGSETREEVLNNLSKFDTLSVTESGILPAINVGSLQPQKALQSDAQ 732
                      T  + S   E++++ L                   G+ +P   L     
Sbjct: 668 -----------TAGVASSLSEKIVDTL-------------------GNSRPGAPL----- 657

Query: 733 QGNSFTLQLFQRTLEETLDSFQRSIHDDMRNLHLEILRQFHMQEMEMSSVMSTILENQAE 792
              S  ++  Q  ++ETLD F+ + H D+ NL +E+++QFH+Q  EM S++     N+  
Sbjct: 728 --TSVQIRFIQNMIQETLDDFREACHRDIVNLQVEMIKQFHIQLNEMHSLLERYSVNEG- 657

Query: 793 LIKEVKSLRKENQQLR 794
           L+ E++ LR+EN++LR
Sbjct: 788 LVAEIERLREENKRLR 657

BLAST of Lag0030312 vs. ExPASy Swiss-Prot
Match: Q8YRI1 (Uncharacterized WD repeat-containing protein alr3466 OS=Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576) OX=103690 GN=alr3466 PE=4 SV=1)

HSP 1 Score: 77.8 bits (190), Expect = 6.2e-13
Identity = 81/289 (28.03%), Postives = 137/289 (47.40%), Query Frame = 0

Query: 9    ALLAASAGD-TVKLFDVSVHSNDLGDPCTLSYTPSPGYQVNSVKWNHTNLVVASAGDDKK 68
            A+LA+ + D TV+L+D+S   N L    TL    S    V SV ++    ++AS GDD+ 
Sbjct: 1045 AMLASGSDDQTVRLWDIS-SGNCL---YTLQGHTS---CVRSVVFSPDGAMLASGGDDQI 1104

Query: 69   ISLWR-KNGQSMGTIPIAGSDSGDNIEESIFSISFSNKVSRYMCTGGSGHVVRIWDLQRK 128
            + LW   +G  + T  + G  S        F +   N V+  +  G S  +VR+WD+  K
Sbjct: 1105 VRLWDISSGNCLYT--LQGYTSWVR-----FLVFSPNGVT--LANGSSDQIVRLWDISSK 1164

Query: 129  RCIKWLRGHTNTITGAMYNCKDEHLASISLSGDLILHNLASGARAAELKDPNEQVLRVLD 188
            +C+  L+GHTN +    ++     LAS S    + L +++S      L+     V  V+ 
Sbjct: 1165 KCLYTLQGHTNWVNAVAFSPDGATLASGSGDQTVRLWDISSSKCLYILQGHTSWVNSVV- 1224

Query: 189  YSRISRHLLVTAGDDGTVHLWDTTGRNPKVSWQKQHSAPTAGIGFSPSNDKTLASVGLDK 248
                    L +   D TV LW+        ++Q  H++    + F+P +   LAS   DK
Sbjct: 1225 -FNPDGSTLASGSSDQTVRLWEINSSKCLCTFQ-GHTSWVNSVVFNP-DGSMLASGSSDK 1284

Query: 249  KLYTYD-SGSRRPSSFIAYEAPFSSMAFRDDGLVLAAGTSNGRVVFYDV 295
             +  +D S S+   +F  +    +S+AF  DG +LA+G+ +  V  +++
Sbjct: 1285 TVRLWDISSSKCLHTFQGHTNWVNSVAFNPDGSMLASGSGDQTVRLWEI 1313

BLAST of Lag0030312 vs. ExPASy TrEMBL
Match: A0A6J1JP94 (protein NEDD1 OS=Cucurbita maxima OX=3661 GN=LOC111487660 PE=4 SV=1)

HSP 1 Score: 1465.7 bits (3793), Expect = 0.0e+00
Identity = 753/800 (94.12%), Postives = 773/800 (96.62%), Query Frame = 0

Query: 1   MNSGDQSMALLAASAGDTVKLFDVSVHSNDLGDPCTLSYTPSPGYQVNSVKWNHTNLVVA 60
           M SGD SMALLAASAGDTVKLFDVS +SNDLGDPCTLSYTPSPGYQVNSVKWNHTNLVVA
Sbjct: 1   MTSGDPSMALLAASAGDTVKLFDVSAYSNDLGDPCTLSYTPSPGYQVNSVKWNHTNLVVA 60

Query: 61  SAGDDKKISLWRKNGQSMGTIPIAGSDSGDNIEESIFSISFSNKVSRYMCTGGSGHVVRI 120
           SAGDDKKISLWRKNGQSMGTIPIAGSD GDNIEESIFSISFSNKVSRYMCTGGSGHVVRI
Sbjct: 61  SAGDDKKISLWRKNGQSMGTIPIAGSDGGDNIEESIFSISFSNKVSRYMCTGGSGHVVRI 120

Query: 121 WDLQRKRCIKWLRGHTNTITGAMYNCKDEHLASISLSGDLILHNLASGARAAELKDPNEQ 180
           WDLQRKRCIKWLRGHTNTITGAMYNCKDEHLASISLSGDL+LHNLASGARAAELKDPNEQ
Sbjct: 121 WDLQRKRCIKWLRGHTNTITGAMYNCKDEHLASISLSGDLLLHNLASGARAAELKDPNEQ 180

Query: 181 VLRVLDYSRISRHLLVTAGDDGTVHLWDTTGRNPKVSWQKQHSAPTAGIGFSPSNDKTLA 240
           VLRVLDYSRISRHLLVTAGDDGTVHLWDTTGRNPKVSWQKQHSAPTAGI FSPSNDKTLA
Sbjct: 181 VLRVLDYSRISRHLLVTAGDDGTVHLWDTTGRNPKVSWQKQHSAPTAGISFSPSNDKTLA 240

Query: 241 SVGLDKKLYTYDSGSRRPSSFIAYEAPFSSMAFRDDGLVLAAGTSNGRVVFYDVRGKPEP 300
           SVGLDKKLYTYDSGSRRPSSFIAYEAPFSSMAFRDDGL+LAAGTSNGRVVFYDVRGKPEP
Sbjct: 241 SVGLDKKLYTYDSGSRRPSSFIAYEAPFSSMAFRDDGLILAAGTSNGRVVFYDVRGKPEP 300

Query: 301 YIVLRAYSSSEAVTSLSWQRLKPVIVNEGNCTAEVALLGGAVEDSILMPDPLPSVTTSTV 360
           YIVLRAYSSSEAVTS+SWQRLKPV VNEGNCTA+VALLGGA+EDSILMPDPLPSVTTSTV
Sbjct: 301 YIVLRAYSSSEAVTSISWQRLKPVNVNEGNCTADVALLGGAIEDSILMPDPLPSVTTSTV 360

Query: 361 ALSTTVSSSRNPGRSGPTFEASLTETSSTFSTAEETPHRSHLRPGGMLARLHAPRSSYNF 420
           ALSTT SSSRNPG SG TFEASLTET ST STAEETPHRSHLRPGG L RLHAPRS+YNF
Sbjct: 361 ALSTTTSSSRNPGHSGLTFEASLTETCSTISTAEETPHRSHLRPGGSLTRLHAPRSNYNF 420

Query: 421 KDDMEVFSPLVDVQPITPSLDKLWDEQNGQKKDHPFDKKPLSLLFPSSSRRFSSIEDGAS 480
           KDDMEVFSPLVDVQPITPSLDKLWD+QNG KKDHPFDKKPL LLFPSSSRR SSIEDG S
Sbjct: 421 KDDMEVFSPLVDVQPITPSLDKLWDDQNGLKKDHPFDKKPLPLLFPSSSRRISSIEDGPS 480

Query: 481 DHPIFNWKSSSSRQDDSRSSSGQLGSTPAPTLNSNNEDSSITPPEAWGGEKLSDKFAQLR 540
           DHPIFNWKSSSS+QDDSRSSSGQLGSTPAPTLNS NEDSSITPPEAWGGEKLSDKFAQLR
Sbjct: 481 DHPIFNWKSSSSKQDDSRSSSGQLGSTPAPTLNSKNEDSSITPPEAWGGEKLSDKFAQLR 540

Query: 541 QPVSLPSRFGMMASGSSSQTSSSMISGLQDPSSSVSQSSITSLTNLNFSYPNLRTKDVTS 600
           QP +LPSRFGM+AS SSSQTSSSMISGLQDPSSS+SQSSITSLTNLNFSYPN+RTKDV S
Sbjct: 541 QPATLPSRFGMLASSSSSQTSSSMISGLQDPSSSISQSSITSLTNLNFSYPNMRTKDVIS 600

Query: 601 QEVSLSIPEHFSTTAASLSLGTRGTIGLSNQDSPRNTTMTLPRRFSTYAERLSTTSSFSD 660
           QEVSLSIPEHFSTTAASLSLGTRGTIGLSNQDSPR TTMTLPRRFSTYAERLSTTSSFSD
Sbjct: 601 QEVSLSIPEHFSTTAASLSLGTRGTIGLSNQDSPRTTTMTLPRRFSTYAERLSTTSSFSD 660

Query: 661 GLP--MGSPKTKKMGSETREEVLNNLSKFDTLSVTESGILPAINVGSLQPQKALQSDAQQ 720
           GLP  +GSPKTKKMGSETREEVLNNLSKFDTLSVTESGILPA+N G LQPQKALQ+DAQQ
Sbjct: 661 GLPVGVGSPKTKKMGSETREEVLNNLSKFDTLSVTESGILPAMNGGPLQPQKALQTDAQQ 720

Query: 721 GNSFTLQLFQRTLEETLDSFQRSIHDDMRNLHLEILRQFHMQEMEMSSVMSTILENQAEL 780
           GNSFTLQLFQRTLEETLDSFQRSIHDDMRNLHLEILRQFHMQEMEMSSV S++L+NQAEL
Sbjct: 721 GNSFTLQLFQRTLEETLDSFQRSIHDDMRNLHLEILRQFHMQEMEMSSVTSSMLKNQAEL 780

Query: 781 IKEVKSLRKENQQLRDLLGS 799
           IKEVKSLRKENQQLR+LLGS
Sbjct: 781 IKEVKSLRKENQQLRNLLGS 800

BLAST of Lag0030312 vs. ExPASy TrEMBL
Match: A0A6J1FHX7 (protein NEDD1-like OS=Cucurbita moschata OX=3662 GN=LOC111445500 PE=4 SV=1)

HSP 1 Score: 1459.5 bits (3777), Expect = 0.0e+00
Identity = 747/800 (93.38%), Postives = 775/800 (96.88%), Query Frame = 0

Query: 1   MNSGDQSMALLAASAGDTVKLFDVSVHSNDLGDPCTLSYTPSPGYQVNSVKWNHTNLVVA 60
           MNSGD SMALLAASAGDTVKLFDVSVHSNDLGDPCTLSYTPSPGYQVNSVKWNHTNLVVA
Sbjct: 1   MNSGDPSMALLAASAGDTVKLFDVSVHSNDLGDPCTLSYTPSPGYQVNSVKWNHTNLVVA 60

Query: 61  SAGDDKKISLWRKNGQSMGTIPIAGSDSGDNIEESIFSISFSNKVSRYMCTGGSGHVVRI 120
           SAGDDKKISLWRKNGQSMGTIPIAG+DSGDNIEESIFSISFS+KVSRYMCTGGSGH+VRI
Sbjct: 61  SAGDDKKISLWRKNGQSMGTIPIAGNDSGDNIEESIFSISFSSKVSRYMCTGGSGHIVRI 120

Query: 121 WDLQRKRCIKWLRGHTNTITGAMYNCKDEHLASISLSGDLILHNLASGARAAELKDPNEQ 180
           WDLQRKRCIKWLRGHTNTITGAMYNCKDEHLASISLSGDLILHNLASGARA ELKDPNEQ
Sbjct: 121 WDLQRKRCIKWLRGHTNTITGAMYNCKDEHLASISLSGDLILHNLASGARATELKDPNEQ 180

Query: 181 VLRVLDYSRISRHLLVTAGDDGTVHLWDTTGRNPKVSWQKQHSAPTAGIGFSPSNDKTLA 240
           VLRVLDYSRISRHLLVTAGDDGTVHLWDTTGRNPKVSWQKQHSAPTAGIGFSPSNDKTLA
Sbjct: 181 VLRVLDYSRISRHLLVTAGDDGTVHLWDTTGRNPKVSWQKQHSAPTAGIGFSPSNDKTLA 240

Query: 241 SVGLDKKLYTYDSGSRRPSSFIAYEAPFSSMAFRDDGLVLAAGTSNGRVVFYDVRGKPEP 300
           SVG+DKKLYTYDSGSRRPSSFIAYEAPFSSMAFRDDGL+LAAGTSNGRVVFYDVRGKPEP
Sbjct: 241 SVGMDKKLYTYDSGSRRPSSFIAYEAPFSSMAFRDDGLILAAGTSNGRVVFYDVRGKPEP 300

Query: 301 YIVLRAYSSSEAVTSLSWQRLKPVIVNEGNCTAEVALLGGAVEDSILMPDPLPSVTTSTV 360
           YIVLRA+SSSEAVTSLSWQRLKPVIVNEGNCTAEVALLGGA+EDSILMPDPLPSVTTST+
Sbjct: 301 YIVLRAFSSSEAVTSLSWQRLKPVIVNEGNCTAEVALLGGAIEDSILMPDPLPSVTTSTI 360

Query: 361 ALSTTVSSSRNPGRSGPTFEASLTETSSTFSTAEETPHRSHLRPGGMLARLHAPRSSYNF 420
            +STT+SSS NPGRSGPTFEASL ETSSTF+TAEETPHRSHL P G LARLHAPRSSYNF
Sbjct: 361 PMSTTISSSWNPGRSGPTFEASLAETSSTFTTAEETPHRSHLWPLGSLARLHAPRSSYNF 420

Query: 421 KDDMEVFSPLVDVQPITPSLDKLWDEQNGQKKDHPFDKKPLSLLFPSSSRRFSSIEDGAS 480
           KDDMEVFSPLVDVQPITPSLDKLWD+QNG KKDHPFDKKPLS+LFPSS+RRFSSIEDGA 
Sbjct: 421 KDDMEVFSPLVDVQPITPSLDKLWDDQNGLKKDHPFDKKPLSMLFPSSNRRFSSIEDGAG 480

Query: 481 DHPIFNWKSSSSRQDDSRSSSGQLGSTPAPTLNSNNEDSSITPPEAWGGEKLSDKFAQLR 540
           DHPIF+WKSSSS+QDDSRSSSGQLGS+P  TLNS +EDSSITPPEAWGGEKLSDKFAQLR
Sbjct: 481 DHPIFHWKSSSSKQDDSRSSSGQLGSSPTSTLNSKSEDSSITPPEAWGGEKLSDKFAQLR 540

Query: 541 QPVSLPSRFGMMA--SGSSSQTSSSMISGLQDPSSSVSQSSITSLTNLNFSYPNLRTKDV 600
           QP++LPSRFGM+A  S SSSQTSSS+ISGLQDPSSSVSQSSITSLTNLNFSYPNLRTKD 
Sbjct: 541 QPMTLPSRFGMLASSSSSSSQTSSSLISGLQDPSSSVSQSSITSLTNLNFSYPNLRTKDA 600

Query: 601 TSQEVSLSIPEHFSTTAASLSLGTRGTIGLSNQDSPRNTTMTLPRRFSTYAERLSTTSSF 660
           TSQEVSLSIPEH STTAASLSLGTRGT+GLSN DSP NTTMTLPRRFSTYAERLSTTSSF
Sbjct: 601 TSQEVSLSIPEHLSTTAASLSLGTRGTVGLSNPDSPWNTTMTLPRRFSTYAERLSTTSSF 660

Query: 661 SDGLPMGSPKTKKMGSETREEVLNNLSKFDTLSVTESGILPAINVGSLQPQKALQSDAQQ 720
           SDGLP+GSPKTKKMGSETREEVLNNLSKFDTLSV ESGILPA+N GSLQPQKALQ D QQ
Sbjct: 661 SDGLPVGSPKTKKMGSETREEVLNNLSKFDTLSVAESGILPAMNGGSLQPQKALQPDGQQ 720

Query: 721 GNSFTLQLFQRTLEETLDSFQRSIHDDMRNLHLEILRQFHMQEMEMSSVMSTILENQAEL 780
           GNSFTLQLFQRTLEETLDSFQRSIHDDMRNLH+EILRQFHMQE+EMSSV S+ILENQAEL
Sbjct: 721 GNSFTLQLFQRTLEETLDSFQRSIHDDMRNLHIEILRQFHMQEIEMSSVTSSILENQAEL 780

Query: 781 IKEVKSLRKENQQLRDLLGS 799
           IKEVKSLRKENQQLRDLLGS
Sbjct: 781 IKEVKSLRKENQQLRDLLGS 800

BLAST of Lag0030312 vs. ExPASy TrEMBL
Match: A0A6J1H7Q0 (protein NEDD1 OS=Cucurbita moschata OX=3662 GN=LOC111461198 PE=4 SV=1)

HSP 1 Score: 1457.6 bits (3772), Expect = 0.0e+00
Identity = 751/800 (93.88%), Postives = 772/800 (96.50%), Query Frame = 0

Query: 1   MNSGDQSMALLAASAGDTVKLFDVSVHSNDLGDPCTLSYTPSPGYQVNSVKWNHTNLVVA 60
           M SGD SMALLAASAGDTVKLFDVS +SNDLGDPCTLSYTPSPGYQVNSVKWNHTNLVVA
Sbjct: 1   MTSGDPSMALLAASAGDTVKLFDVSAYSNDLGDPCTLSYTPSPGYQVNSVKWNHTNLVVA 60

Query: 61  SAGDDKKISLWRKNGQSMGTIPIAGSDSGDNIEESIFSISFSNKVSRYMCTGGSGHVVRI 120
           SAGDDKKISLWRKNGQSMGTIPIAGSD GDNIEESIFSISFSNKVSRYMCTGGSGHVVRI
Sbjct: 61  SAGDDKKISLWRKNGQSMGTIPIAGSDGGDNIEESIFSISFSNKVSRYMCTGGSGHVVRI 120

Query: 121 WDLQRKRCIKWLRGHTNTITGAMYNCKDEHLASISLSGDLILHNLASGARAAELKDPNEQ 180
           WDLQRKRCIKWLRGHTNTITGAMYNCKDEHLASISLSGDL+LHNLASGARAAELKDPNEQ
Sbjct: 121 WDLQRKRCIKWLRGHTNTITGAMYNCKDEHLASISLSGDLLLHNLASGARAAELKDPNEQ 180

Query: 181 VLRVLDYSRISRHLLVTAGDDGTVHLWDTTGRNPKVSWQKQHSAPTAGIGFSPSNDKTLA 240
           VLRVLDYSRISRHLLVTAGDDGTVHLWDTTGRNPKVSWQKQHSAPTAGI FSPSNDKTLA
Sbjct: 181 VLRVLDYSRISRHLLVTAGDDGTVHLWDTTGRNPKVSWQKQHSAPTAGISFSPSNDKTLA 240

Query: 241 SVGLDKKLYTYDSGSRRPSSFIAYEAPFSSMAFRDDGLVLAAGTSNGRVVFYDVRGKPEP 300
           SVGLDKKLYTYDSGSRRPSSFIAYEAPFSSMAFRDDGL+LAAGTSNGRVVFYDVRGKPEP
Sbjct: 241 SVGLDKKLYTYDSGSRRPSSFIAYEAPFSSMAFRDDGLILAAGTSNGRVVFYDVRGKPEP 300

Query: 301 YIVLRAYSSSEAVTSLSWQRLKPVIVNEGNCTAEVALLGGAVEDSILMPDPLPSVTTSTV 360
           YIVLRAYSSSEAVTS+SWQRLKPVIVNEGNCTA+VALLGGA+EDSILMPDPLPSVTTSTV
Sbjct: 301 YIVLRAYSSSEAVTSISWQRLKPVIVNEGNCTADVALLGGAIEDSILMPDPLPSVTTSTV 360

Query: 361 ALSTTVSSSRNPGRSGPTFEASLTETSSTFSTAEETPHRSHLRPGGMLARLHAPRSSYNF 420
           ALSTT SSSRNPG SG TFEASLTETSST STAEETPHRSHLRPGG L RLHAPRS+YNF
Sbjct: 361 ALSTTTSSSRNPGHSGLTFEASLTETSSTISTAEETPHRSHLRPGGSLTRLHAPRSNYNF 420

Query: 421 KDDMEVFSPLVDVQPITPSLDKLWDEQNGQKKDHPFDKKPLSLLFPSSSRRFSSIEDGAS 480
           KDDMEVFSPLVDVQPITPSLDKLWD+QNG KKDHPFDKKPL LLFPSSSRRFSSIEDG S
Sbjct: 421 KDDMEVFSPLVDVQPITPSLDKLWDDQNGLKKDHPFDKKPLPLLFPSSSRRFSSIEDGPS 480

Query: 481 DHPIFNWKSSSSRQDDSRSSSGQLGSTPAPTLNSNNEDSSITPPEAWGGEKLSDKFAQLR 540
           DHPIFNWKSSSS+QDDSRSSSGQLGSTPAPTLNS NEDSSITPPEAWGGEKLSDKFAQLR
Sbjct: 481 DHPIFNWKSSSSKQDDSRSSSGQLGSTPAPTLNSKNEDSSITPPEAWGGEKLSDKFAQLR 540

Query: 541 QPVSLPSRFGMMASGSSSQTSSSMISGLQDPSSSVSQSSITSLTNLNFSYPNLRTKDVTS 600
           QP +LPSRFGM+AS SSSQTSSSMISGLQDP SS+SQSSITSLTNLNFSYPN+RTKDV S
Sbjct: 541 QPATLPSRFGMLASSSSSQTSSSMISGLQDP-SSISQSSITSLTNLNFSYPNMRTKDVIS 600

Query: 601 QEVSLSIPEHFSTTAASLSLGTRGTIGLSNQDSPRNTTMTLPRRFSTYAERLSTTSSFSD 660
           QEVSLSIPEHFST AASLSLGTRGTIGLSNQDSPR TTMTLPRRFSTYAERLSTTSSFSD
Sbjct: 601 QEVSLSIPEHFSTAAASLSLGTRGTIGLSNQDSPRTTTMTLPRRFSTYAERLSTTSSFSD 660

Query: 661 GLP--MGSPKTKKMGSETREEVLNNLSKFDTLSVTESGILPAINVGSLQPQKALQSDAQQ 720
           GLP  +GSPKTKKMGSETREEVLNNLSKFDTLSVTESGILPA+N G L  QKALQ+DAQQ
Sbjct: 661 GLPVGVGSPKTKKMGSETREEVLNNLSKFDTLSVTESGILPAMNGGPLLQQKALQTDAQQ 720

Query: 721 GNSFTLQLFQRTLEETLDSFQRSIHDDMRNLHLEILRQFHMQEMEMSSVMSTILENQAEL 780
           GNSFTLQLFQRTLEETLDSFQRSIHDDMRNLHLEILRQFH+QEMEMSSV S++L+NQAEL
Sbjct: 721 GNSFTLQLFQRTLEETLDSFQRSIHDDMRNLHLEILRQFHVQEMEMSSVTSSMLKNQAEL 780

Query: 781 IKEVKSLRKENQQLRDLLGS 799
           IKEVKSLRKENQQLR+LLGS
Sbjct: 781 IKEVKSLRKENQQLRNLLGS 799

BLAST of Lag0030312 vs. ExPASy TrEMBL
Match: A0A6J1JX78 (protein NEDD1-like OS=Cucurbita maxima OX=3661 GN=LOC111489662 PE=4 SV=1)

HSP 1 Score: 1453.3 bits (3761), Expect = 0.0e+00
Identity = 746/800 (93.25%), Postives = 773/800 (96.62%), Query Frame = 0

Query: 1   MNSGDQSMALLAASAGDTVKLFDVSVHSNDLGDPCTLSYTPSPGYQVNSVKWNHTNLVVA 60
           MNSGD SMALLAASAGDTVKLFDVSVHSNDLGDPCTLSYTPSPGYQVNSVKWNHTNLVVA
Sbjct: 1   MNSGDPSMALLAASAGDTVKLFDVSVHSNDLGDPCTLSYTPSPGYQVNSVKWNHTNLVVA 60

Query: 61  SAGDDKKISLWRKNGQSMGTIPIAGSDSGDNIEESIFSISFSNKVSRYMCTGGSGHVVRI 120
           SAGDDKKISLWRKNGQSMGTIPI G+DSGDNIEESIFSISFS+KVSRYMCTGGSGHVVRI
Sbjct: 61  SAGDDKKISLWRKNGQSMGTIPITGNDSGDNIEESIFSISFSSKVSRYMCTGGSGHVVRI 120

Query: 121 WDLQRKRCIKWLRGHTNTITGAMYNCKDEHLASISLSGDLILHNLASGARAAELKDPNEQ 180
           WDLQRKRCIKWLRGHTNTITGAMYNCKDEHLASISLSGDLILHNLASGARA ELKDPNEQ
Sbjct: 121 WDLQRKRCIKWLRGHTNTITGAMYNCKDEHLASISLSGDLILHNLASGARATELKDPNEQ 180

Query: 181 VLRVLDYSRISRHLLVTAGDDGTVHLWDTTGRNPKVSWQKQHSAPTAGIGFSPSNDKTLA 240
           VLRVLDYSRISRHLLVTAGDDGTV LWDTTGRNPKVSWQKQHSAPTAGIGFSPSNDKTLA
Sbjct: 181 VLRVLDYSRISRHLLVTAGDDGTVLLWDTTGRNPKVSWQKQHSAPTAGIGFSPSNDKTLA 240

Query: 241 SVGLDKKLYTYDSGSRRPSSFIAYEAPFSSMAFRDDGLVLAAGTSNGRVVFYDVRGKPEP 300
           SVG+DKKLYTYDSGSRRPSSFIAYEAPFSSMAFRDDGL+LAAGTSNGRVVFYDVRGKPEP
Sbjct: 241 SVGMDKKLYTYDSGSRRPSSFIAYEAPFSSMAFRDDGLILAAGTSNGRVVFYDVRGKPEP 300

Query: 301 YIVLRAYSSSEAVTSLSWQRLKPVIVNEGNCTAEVALLGGAVEDSILMPDPLPSVTTSTV 360
           YIVLRA+SSSEAVTSLSWQRLKPVIVNEGNCTAEVALLGGA+EDSILMPDPLPSVTTST+
Sbjct: 301 YIVLRAFSSSEAVTSLSWQRLKPVIVNEGNCTAEVALLGGAIEDSILMPDPLPSVTTSTI 360

Query: 361 ALSTTVSSSRNPGRSGPTFEASLTETSSTFSTAEETPHRSHLRPGGMLARLHAPRSSYNF 420
            +STT+SSSRNPGRSGPTFEASL ETSSTF+TAEETPHRSHL P G LARLHAPRSSYNF
Sbjct: 361 PMSTTISSSRNPGRSGPTFEASLAETSSTFTTAEETPHRSHLWPLGSLARLHAPRSSYNF 420

Query: 421 KDDMEVFSPLVDVQPITPSLDKLWDEQNGQKKDHPFDKKPLSLLFPSSSRRFSSIEDGAS 480
           KDDMEVFSPLVDVQPITPSLDKLWD+QNG KKDHPFDKKPLS+LFPSS+RRFSS EDGA 
Sbjct: 421 KDDMEVFSPLVDVQPITPSLDKLWDDQNGLKKDHPFDKKPLSMLFPSSNRRFSSTEDGAG 480

Query: 481 DHPIFNWKSSSSRQDDSRSSSGQLGSTPAPTLNSNNEDSSITPPEAWGGEKLSDKFAQLR 540
           DHPIF+WKSSSS+QDDSRSSSGQLGS+P  TLNS +EDSSITPPEAWGGEKLSDKFAQLR
Sbjct: 481 DHPIFHWKSSSSKQDDSRSSSGQLGSSPTSTLNSKSEDSSITPPEAWGGEKLSDKFAQLR 540

Query: 541 QPVSLPSRFGMMA--SGSSSQTSSSMISGLQDPSSSVSQSSITSLTNLNFSYPNLRTKDV 600
           QP++LPSRFGM+A  S SSSQT SS+ISGLQDPSSSVSQSSITSLTNLNFSYPNLRTKD 
Sbjct: 541 QPMTLPSRFGMLASSSSSSSQTPSSLISGLQDPSSSVSQSSITSLTNLNFSYPNLRTKDA 600

Query: 601 TSQEVSLSIPEHFSTTAASLSLGTRGTIGLSNQDSPRNTTMTLPRRFSTYAERLSTTSSF 660
           TSQEVSLSIPEH STTAASLSLGTRGTIGLSN DSP  TTMTLPRRFSTYAERLSTTSSF
Sbjct: 601 TSQEVSLSIPEHLSTTAASLSLGTRGTIGLSNPDSPWTTTMTLPRRFSTYAERLSTTSSF 660

Query: 661 SDGLPMGSPKTKKMGSETREEVLNNLSKFDTLSVTESGILPAINVGSLQPQKALQSDAQQ 720
           SDGLP+GSPKTKKMGSETREEVLNNLSKFDTLSVTESGILPA+N GSLQPQKALQ DAQQ
Sbjct: 661 SDGLPVGSPKTKKMGSETREEVLNNLSKFDTLSVTESGILPAMNGGSLQPQKALQPDAQQ 720

Query: 721 GNSFTLQLFQRTLEETLDSFQRSIHDDMRNLHLEILRQFHMQEMEMSSVMSTILENQAEL 780
           GNSFTL+LFQRTLEETLDSFQRSIHDDMRNLH+EILRQFHMQE+EMSSV S+ILENQAEL
Sbjct: 721 GNSFTLKLFQRTLEETLDSFQRSIHDDMRNLHIEILRQFHMQEIEMSSVTSSILENQAEL 780

Query: 781 IKEVKSLRKENQQLRDLLGS 799
           IKEVKSLRKENQQLRDLLGS
Sbjct: 781 IKEVKSLRKENQQLRDLLGS 800

BLAST of Lag0030312 vs. ExPASy TrEMBL
Match: A0A0A0KA83 (WD_REPEATS_REGION domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G008800 PE=4 SV=1)

HSP 1 Score: 1446.4 bits (3743), Expect = 0.0e+00
Identity = 748/800 (93.50%), Postives = 768/800 (96.00%), Query Frame = 0

Query: 1   MNSGDQSMALLAASAGDTVKLFDVSVHSNDLGDPCTLSYTPSPGYQVNSVKWNHTNLVVA 60
           MNSGD SMALLAASAGDTVKLFDVSVHSNDLGDPCTLSYTPSPGYQVNSVKWNHTNLVVA
Sbjct: 1   MNSGDPSMALLAASAGDTVKLFDVSVHSNDLGDPCTLSYTPSPGYQVNSVKWNHTNLVVA 60

Query: 61  SAGDDKKISLWRKNGQSMGTIPIAGSDSGDNIEESIFSISFSNKVSRYMCTGGSGHVVRI 120
           SAGDDKKISLWRKNGQSMGTIPIAG+DSGDNIEESIFSISF NKVSRYMCTGGSGHVVRI
Sbjct: 61  SAGDDKKISLWRKNGQSMGTIPIAGTDSGDNIEESIFSISFGNKVSRYMCTGGSGHVVRI 120

Query: 121 WDLQRKRCIKWLRGHTNTITGAMYNCKDEHLASISLSGDLILHNLASGARAAELKDPNEQ 180
           WDLQRKRCIKWLRGHTNTITGAMYNCKDEHLASISLSGDLILHNLASGARAAELKDPNEQ
Sbjct: 121 WDLQRKRCIKWLRGHTNTITGAMYNCKDEHLASISLSGDLILHNLASGARAAELKDPNEQ 180

Query: 181 VLRVLDYSRISRHLLVTAGDDGTVHLWDTTGRNPKVSWQKQHSAPTAGIGFSPSNDKTLA 240
           VLRVLDYSR SRHLLVTAGDDGTVHLWDTTGRNPK+SWQKQHSAPTAGIGFSPSNDK+ A
Sbjct: 181 VLRVLDYSRTSRHLLVTAGDDGTVHLWDTTGRNPKISWQKQHSAPTAGIGFSPSNDKSFA 240

Query: 241 SVGLDKKLYTYDSGSRRPSSFIAYEAPFSSMAFRDDGLVLAAGTSNGRVVFYDVRGKPEP 300
           SVGLDKKLYTYDSGSRRPSSFIAYEAPFSSMAFRDDGL+LAAGTSNGRVVFYDVRGKPEP
Sbjct: 241 SVGLDKKLYTYDSGSRRPSSFIAYEAPFSSMAFRDDGLILAAGTSNGRVVFYDVRGKPEP 300

Query: 301 YIVLRAYSSSEAVTSLSWQRLKPVIVNEGNCTAEVALLGGAVEDSILMPDPLPSVTTSTV 360
           YIVLRAYSSSEAVTSLSWQRLKPVIVNE NCTAEVALLGGA+EDSILMPDPLPSVTTST 
Sbjct: 301 YIVLRAYSSSEAVTSLSWQRLKPVIVNESNCTAEVALLGGAIEDSILMPDPLPSVTTSTG 360

Query: 361 ALSTTVSSSRNPGRSGPTFEASLTETSSTFSTAEETPHRSHLRPGG--MLARLHAPRSSY 420
            LS T S SRNPGRSG TFEASLTETSS+FSTAEETP RSHLR GG   LARLHAPRSSY
Sbjct: 361 PLSAT-SGSRNPGRSGSTFEASLTETSSSFSTAEETPLRSHLRSGGSLALARLHAPRSSY 420

Query: 421 NFKDDMEVFSPLVDVQPITPSLDKLWDEQNGQKKDHPFDKKPLSLLFPSSSRRFSSIEDG 480
           NFKDDMEVFSPLVDVQPITPSLDKLWD+ NG KKD+PFDKKPLS+LFPSSSRRFSSIEDG
Sbjct: 421 NFKDDMEVFSPLVDVQPITPSLDKLWDDHNGSKKDNPFDKKPLSMLFPSSSRRFSSIEDG 480

Query: 481 ASDHPIFNWKSSSSRQDDSRSSSGQLGSTPAPTLNSNNEDSSITPPEAWGGEKLSDKFAQ 540
           ASDHPIFNWKSSSS+QDD RSSSGQLGSTPAPT+NS NEDSSITPPEAWGGEKLS+KFAQ
Sbjct: 481 ASDHPIFNWKSSSSKQDDIRSSSGQLGSTPAPTVNSKNEDSSITPPEAWGGEKLSEKFAQ 540

Query: 541 LRQPVSLPSRFGMMASGSSSQTSSSMISGLQDPSSSVSQSSITSLTNLNFSYPNLRTKDV 600
           LRQP++LPSRFGM+AS SSSQTSSSMISGLQDPSSS+SQSSITSLTNLNFSYPNLRTKD 
Sbjct: 541 LRQPMTLPSRFGMLASSSSSQTSSSMISGLQDPSSSISQSSITSLTNLNFSYPNLRTKDA 600

Query: 601 TSQEVSLSIPEHFSTTAASLSLGTRGTIGLSNQDSPRNTTMTLPRRFSTYAERLSTTSSF 660
           TSQEVSLSIPEHFSTTAASLSLGTR  IGLSN DSPR +TMTLPRRFSTYAERLSTTSSF
Sbjct: 601 TSQEVSLSIPEHFSTTAASLSLGTRVNIGLSNTDSPRPSTMTLPRRFSTYAERLSTTSSF 660

Query: 661 SDGLPMGSPKTKKMGSETREEVLNNLSKFDTLSVTESGILPAINVGSLQPQKALQSDAQQ 720
           SDGLP GSPKTKK+GSETREEVLNNL+KFDTLSVTESGI PA+N G LQPQK LQSDAQQ
Sbjct: 661 SDGLPAGSPKTKKLGSETREEVLNNLAKFDTLSVTESGIPPAMNGGLLQPQKPLQSDAQQ 720

Query: 721 GNSFTLQLFQRTLEETLDSFQRSIHDDMRNLHLEILRQFHMQEMEMSSVMSTILENQAEL 780
           GNSFTLQLFQRTLEETLDSFQRSIHDDMRNLHLEILRQFHMQEMEMSSV STILENQAEL
Sbjct: 721 GNSFTLQLFQRTLEETLDSFQRSIHDDMRNLHLEILRQFHMQEMEMSSVTSTILENQAEL 780

Query: 781 IKEVKSLRKENQQLRDLLGS 799
           IKEVKSLRKENQQLRDLLGS
Sbjct: 781 IKEVKSLRKENQQLRDLLGS 799

BLAST of Lag0030312 vs. TAIR 10
Match: AT5G05970.2 (Transducin/WD40 repeat-like superfamily protein )

HSP 1 Score: 869.0 bits (2244), Expect = 2.9e-252
Identity = 483/813 (59.41%), Postives = 593/813 (72.94%), Query Frame = 0

Query: 2   NSGDQSMALLAASAGDTVKLFDVSVHSNDLGDPCTLSYTPSPGYQVNSVKWNHTNLVVAS 61
           N  + S  LLAAS GDTVKLFDVS    D GDPC LSYTPSPG  VNSVKWNHTNLVVAS
Sbjct: 4   NLVEPSWRLLAASGGDTVKLFDVSA---DSGDPCVLSYTPSPGCAVNSVKWNHTNLVVAS 63

Query: 62  AGDDKKISLWRKNGQSMGTIPIAGSDSGDNIEESIFSISFSNKVSRYMCTGGSGHVVRIW 121
            G+DKKISLWRKNGQS+GT+P+ G D GD+ EE + +ISFS K SRY+C+GG+G +V+IW
Sbjct: 64  TGEDKKISLWRKNGQSLGTVPVTGKDGGDSAEECLSAISFSKKGSRYICSGGTGQIVKIW 123

Query: 122 DLQRKRCIKWLRGHTNTITGAMYNCKDEHLASISLSGDLILHNLASGARAAELKDPNEQV 181
           DLQRK CIK L+GHT+TITG MYNCKDEHLAS+S+ GDLI+HNLASGARA ELKDPN QV
Sbjct: 124 DLQRKLCIKKLKGHTSTITGVMYNCKDEHLASVSVGGDLIVHNLASGARATELKDPNGQV 183

Query: 182 LRVLDYSRISRHLLVTAGDDGTVHLWDTTGRNPKVSWQKQHSAPTAGIGFSPSNDKTLAS 241
           LR+LDYSR SRHLLVTAGDDGTVHLWDTTGR+PK+SW KQHSAPTAG+ FSPSN+K +AS
Sbjct: 184 LRLLDYSRSSRHLLVTAGDDGTVHLWDTTGRSPKMSWLKQHSAPTAGVCFSPSNEKIIAS 243

Query: 242 VGLDKKLYTYDSGSRRPSSFIAYEAPFSSMAFRDDGLVLAAGTSNGRVVFYDVRGKPEPY 301
           VG+DKKLYTYDSGSRR SS IAYEAPFSS+AF D+G +L AGTSNGRVVFYD+RGKP+P 
Sbjct: 244 VGMDKKLYTYDSGSRRSSSCIAYEAPFSSLAFGDNGYILVAGTSNGRVVFYDIRGKPQPV 303

Query: 302 IVLRAYSSSEAVTSLSWQRLKPVIVNEGNCTAEVALLGGAVEDSILMPDPLPSVTTSTVA 361
            VL A+S+SE VTSLSWQ  KPVIVNE N T+E+ALLG  VEDS+++PDPLPS T S   
Sbjct: 304 TVLHAFSNSEDVTSLSWQTSKPVIVNEKNYTSEMALLGSTVEDSVVIPDPLPSTTPS--- 363

Query: 362 LSTTVSSSRNPGRSGPTFEASLTETSSTFSTAEETPHRSHLRPGGMLARLHAPRSSYNFK 421
                 S+  PG  G      ++ ++   S+ E+TP+R+HL P G L RLHA R++ ++ 
Sbjct: 364 ---ASQSAMAPGSRG------VSASTVNASSVEQTPNRNHLWPSGPLGRLHALRANDSYN 423

Query: 422 DDMEVFSPLVDVQPITPSLDKLWDEQNGQKKDH-PFDKKPLSLLFPSSSRRFSSIEDGAS 481
           DDM VFSP++DV     S++K  D +    KDH   D KP SLLFPSSS+ +S  ++G+ 
Sbjct: 424 DDMGVFSPIIDVS----SVEKWADSEGYNNKDHLVVDNKPSSLLFPSSSKGYSFGDNGSK 483

Query: 482 DHPIFNWK-SSSSRQDDSRSSSGQLGSTPAPTLNSNNEDSSITPPEAWGGEKLSDKFAQL 541
           +HPIF+WK SS+S+QDD R++    GS   PT +S +EDS++TPPEAWGG+K S+KF QL
Sbjct: 484 EHPIFDWKPSSTSKQDDPRAAFSSFGSI-TPTASSKSEDSALTPPEAWGGDKFSEKFNQL 543

Query: 542 RQP--------VSLPSRFGMMASGSSSQTSSSMISGLQDPSSSVSQSSITSLTNLNFSYP 601
                      +  PSR  + ++G+S  TS SM S  +D   S  Q   T+  N +  +P
Sbjct: 544 ANEKFSDKFSHLHAPSRLAVSSTGAS--TSGSMFSSSRDFPLSHGQ---TNFANASLEFP 603

Query: 602 NLR----TKDVTSQEVSLSIPEHFSTTAASLSLGTRGTIGLSNQDSPRNTTMTLPRRFST 661
            +R    T + +S +   ++P        S  L T+G     N DS R  +    RRFST
Sbjct: 604 RIRDFSSTFETSSTQTDNNLP--------SSPLFTKGITAPGNIDSLR-LSPNFTRRFST 663

Query: 662 YAERLSTTSSFSDGLPM---GSPKTKKMGSETREEVLNN-LSKFDTLSVTESGILPAINV 721
           YAER+STTSSFSDG  +   GSPK KK GSETREEVLN+ L++ +T+  TE+G +P +N 
Sbjct: 664 YAERISTTSSFSDGASLSLGGSPKIKKTGSETREEVLNHLLARPETVVATEAGAMPLMNQ 723

Query: 722 GSLQPQKALQSDAQQGNSFTLQLFQRTLEETLDSFQRSIHDDMRNLHLEILRQFHMQEME 781
           G L+  +  Q      ++FTLQLFQRTLE TLDSFQ SIHDD+RNLH+EILRQFHM EME
Sbjct: 724 GGLKQSQTDQQQVMGSSNFTLQLFQRTLEGTLDSFQNSIHDDVRNLHIEILRQFHMHEME 782

Query: 782 MSSVMSTILENQAELIKEVKSLRKENQQLRDLL 797
           MS V+S+ILENQAE +KE+K LRKENQ+LR  L
Sbjct: 784 MSKVLSSILENQAEQMKELKLLRKENQELRQRL 782

BLAST of Lag0030312 vs. TAIR 10
Match: AT5G05970.1 (Transducin/WD40 repeat-like superfamily protein )

HSP 1 Score: 865.1 bits (2234), Expect = 4.3e-251
Identity = 483/813 (59.41%), Postives = 593/813 (72.94%), Query Frame = 0

Query: 2   NSGDQSMALLAASAGDTVKLFDVSVHSNDLGDPCTLSYTPSPGYQVNSVKWNHTNLVVAS 61
           N  + S  LLAAS GDTVKLFDVS    D GDPC LSYTPSPG  VNSVKWNHTNLVVAS
Sbjct: 4   NLVEPSWRLLAASGGDTVKLFDVSA---DSGDPCVLSYTPSPGCAVNSVKWNHTNLVVAS 63

Query: 62  AGDDKKISLWRKNGQSMGTIPIAGSDSGDNIEESIFSISFSNKVSRYMCTGGSGHVVRIW 121
            G+DKKISLWRKNGQS+GT+P+ G D GD+ EE + +ISFS K SRY+C+GG+G +V+IW
Sbjct: 64  TGEDKKISLWRKNGQSLGTVPVTGKDGGDSAEECLSAISFSKKGSRYICSGGTGQIVKIW 123

Query: 122 DLQRKRCIKWLRGHTNTITGAMYNCKDEHLASISLSGDLILHNLASGARAAELKDPNEQV 181
           DLQRK CIK L+GHT+TITG MYNCKDEHLAS+S+ GDLI+HNLASGARA ELKDPN QV
Sbjct: 124 DLQRKLCIKKLKGHTSTITGVMYNCKDEHLASVSVGGDLIVHNLASGARATELKDPNGQV 183

Query: 182 LRVLDYSRISRHLLVTAGDDGTVHLWDTTGRNPKVSWQKQHSAPTAGIGFSPSNDKTLAS 241
           LR+LDYSR SRHLLVTAGDDGTVHLWDTTGR+PK+SW KQHSAPTAG+ FSPSN+K +AS
Sbjct: 184 LRLLDYSRSSRHLLVTAGDDGTVHLWDTTGRSPKMSWLKQHSAPTAGVCFSPSNEKIIAS 243

Query: 242 VGLDKKLYTYDSGSRRPSSFIAYEAPFSSMAFRDDGLVLAAGTSNGRVVFYDVRGKPEPY 301
           VG+DKKLYTYDSGSRR SS IAYEAPFSS+AF D+G +L AGTSNGRVVFYD+RGKP+P 
Sbjct: 244 VGMDKKLYTYDSGSRRSSSCIAYEAPFSSLAFGDNGYILVAGTSNGRVVFYDIRGKPQPV 303

Query: 302 IVLRAYSSSEAVTSLSWQRLKPVIVNEGNCTAEVALLGGAVEDSILMPDPLPSVTTSTVA 361
            VL A+S+SE VTSLSWQ  KPVIVNE N T+E+ALLG  VEDS+++PDPLPS T S   
Sbjct: 304 TVLHAFSNSEDVTSLSWQTSKPVIVNEKNYTSEMALLGSTVEDSVVIPDPLPSTTPS--- 363

Query: 362 LSTTVSSSRNPGRSGPTFEASLTETSSTFSTAEETPHRSHLRPGGMLARLHAPRSSYNFK 421
                 S+  PG  G      ++ ++   S+ E+TP+R+HL P G L RLHA R++ ++ 
Sbjct: 364 ---ASQSAMAPGSRG------VSASTVNASSVEQTPNRNHLWPSGPLGRLHALRANDSYN 423

Query: 422 DDMEVFSPLVDVQPITPSLDKLWDEQNGQKKDH-PFDKKPLSLLFPSSSRRFSSIEDGAS 481
           DDM VFSP++DV     S++K  D +    KDH   D KP SLLFPSSS+ +S  ++G+ 
Sbjct: 424 DDMGVFSPIIDVS----SVEKWADSEGYNNKDHLVVDNKPSSLLFPSSSKGYSFGDNGSK 483

Query: 482 DHPIFNWK-SSSSRQDDSRSSSGQLGSTPAPTLNSNNEDSSITPPEAWGGEKLSDKFAQL 541
           +HPIF+WK SS+S+QDD R++    GS   PT +S +EDS++TPPEAWGG+K S+KF QL
Sbjct: 484 EHPIFDWKPSSTSKQDDPRAAFSSFGSI-TPTASSKSEDSALTPPEAWGGDKFSEKFNQL 543

Query: 542 RQP--------VSLPSRFGMMASGSSSQTSSSMISGLQDPSSSVSQSSITSLTNLNFSYP 601
                      +  PSR  + ++G+S  TS SM S  +D   S  Q   T+  N +  +P
Sbjct: 544 ANEKFSDKFSHLHAPSRLAVSSTGAS--TSGSMFSSSRDFPLSHGQ---TNFANASLEFP 603

Query: 602 NLR----TKDVTSQEVSLSIPEHFSTTAASLSLGTRGTIGLSNQDSPRNTTMTLPRRFST 661
            +R    T + +S +   ++P        S  L T+G     N DS R  +    RRFST
Sbjct: 604 RIRDFSSTFETSSTQTDNNLP--------SSPLFTKGITAPGNIDSLR-LSPNFTRRFST 663

Query: 662 YAERLSTTSSFSDGLPM---GSPKTKKMGSETREEVLNN-LSKFDTLSVTESGILPAINV 721
           YAER+STTSSFSDG  +   GSPK KK GSETREEVLN+ L++ +T+  TE+G +P +N 
Sbjct: 664 YAERISTTSSFSDGASLSLGGSPKIKKTGSETREEVLNHLLARPETVVATEAGAMPLMN- 723

Query: 722 GSLQPQKALQSDAQQGNSFTLQLFQRTLEETLDSFQRSIHDDMRNLHLEILRQFHMQEME 781
           G L+  +  Q      ++FTLQLFQRTLE TLDSFQ SIHDD+RNLH+EILRQFHM EME
Sbjct: 724 GGLKQSQTDQQQVMGSSNFTLQLFQRTLEGTLDSFQNSIHDDVRNLHIEILRQFHMHEME 781

Query: 782 MSSVMSTILENQAELIKEVKSLRKENQQLRDLL 797
           MS V+S+ILENQAE +KE+K LRKENQ+LR  L
Sbjct: 784 MSKVLSSILENQAEQMKELKLLRKENQELRQRL 781

BLAST of Lag0030312 vs. TAIR 10
Match: AT3G49660.1 (Transducin/WD40 repeat-like superfamily protein )

HSP 1 Score: 65.9 bits (159), Expect = 1.7e-10
Identity = 56/251 (22.31%), Postives = 109/251 (43.43%), Query Frame = 0

Query: 10  LLAASAGDTVKLFDVSVHSNDLGDPCTLSYTPSPGYQ--VNSVKWNHTNLVVASAGDDKK 69
           L +ASA  T++ + +    N + DP         G++  ++ V ++     + SA DDK 
Sbjct: 39  LASASADKTIRTYTI----NTINDPIAEPVQEFTGHENGISDVAFSSDARFIVSASDDKT 98

Query: 70  ISLWRKNGQSMGTIPIAGSDSGDNIEESIFSISFSNKVSRYMCTGGSGHVVRIWDLQRKR 129
           + LW     S+    I  ++         F ++F N  S  + +G     VRIWD+   +
Sbjct: 99  LKLWDVETGSLIKTLIGHTNYA-------FCVNF-NPQSNMIVSGSFDETVRIWDVTTGK 158

Query: 130 CIKWLRGHTNTITGAMYNCKDEHLASISLSGDLILHNLASGARAAELKDPNEQVLRVLDY 189
           C+K L  H++ +T   +N     + S S  G   + +  +G     L D     +  + +
Sbjct: 159 CLKVLPAHSDPVTAVDFNRDGSLIVSSSYDGLCRIWDSGTGHCVKTLIDDENPPVSFVRF 218

Query: 190 SRISRHLLVTAGDDGTVHLWD-TTGRNPKVSWQKQHSAPTAGIGFSPSNDKTLASVGLDK 249
           S   + +LV   D+ T+ LW+ ++ +  K      ++       FS +N K + S   D 
Sbjct: 219 SPNGKFILVGTLDN-TLRLWNISSAKFLKTYTGHVNAQYCISSAFSVTNGKRIVSGSEDN 276

Query: 250 KLYTYDSGSRR 258
            ++ ++  S++
Sbjct: 279 CVHMWELNSKK 276

BLAST of Lag0030312 vs. TAIR 10
Match: AT1G11160.1 (Transducin/WD40 repeat-like superfamily protein )

HSP 1 Score: 65.1 bits (157), Expect = 3.0e-10
Identity = 65/281 (23.13%), Postives = 117/281 (41.64%), Query Frame = 0

Query: 55  TNLVVASAGDDKKISLWRKNGQSMGTIPIAGSDSGDNIEESIFSISFSNKVSRYMCTGGS 114
           T+ ++ + GDD K++LW   G++   + + G  S       + S++F N     +  G S
Sbjct: 27  TSRLLLTGGDDYKVNLW-SIGKTTSPMSLCGHTS------PVDSVAF-NSEEVLVLAGAS 86

Query: 115 GHVVRIWDLQRKRCIKWLRGHTNTITGAMYNCKDEHLASISLSGDLILHNLASGARAAEL 174
             V+++WDL+  + ++   GH +  +   ++   E LAS S   +L + +          
Sbjct: 87  SGVIKLWDLEESKMVRAFTGHRSNCSAVEFHPFGEFLASGSSDTNLRVWDTRKKGCIQTY 146

Query: 175 KDPNEQVLRVLDYSRISRHLLVTAGDDGTVHLWDTTGRNPKVSWQKQHSAPTAGIGFSPS 234
           K  + + +  +++S   R  +V+ G D  V +WD T     +   K H  P   + F P 
Sbjct: 147 KG-HTRGISTIEFSPDGR-WVVSGGLDNVVKVWDLTA-GKLLHEFKCHEGPIRSLDFHPL 206

Query: 235 NDKTLASVGLDKKLYTYD------SGSRRPSSFIAYEAPFSSMAFRDDGLVLAAGTSNGR 294
            +  LA+   D+ +  +D       G+ RP +         ++AF  DG  L  G  +G 
Sbjct: 207 -EFLLATGSADRTVKFWDLETFELIGTTRPEA-----TGVRAIAFHPDGQTLFCGLDDGL 266

Query: 295 VVFYDVRGKPEPYIVLRAYSSSEAVTSLSWQRLKPVIVNEG 330
            V+       EP I             + W  L    +NEG
Sbjct: 267 KVY-----SWEPVICRDG-------VDMGWSTLGDFCINEG 278

BLAST of Lag0030312 vs. TAIR 10
Match: AT5G16750.1 (Transducin family protein / WD-40 repeat family protein )

HSP 1 Score: 61.6 bits (148), Expect = 3.3e-09
Identity = 33/149 (22.15%), Postives = 70/149 (46.98%), Query Frame = 0

Query: 107 RYMCTGGSGHVVRIWDLQRKRCIKWLRGHTNTITGAMYNCKDEHLASISLSGDLILHNLA 166
           + + + G    +R+WDL+  +CI+  +GH   + G   +     LA+      +++ ++ 
Sbjct: 73  KLLFSAGHSRQIRVWDLETLKCIRSWKGHEGPVMGMACHASGGLLATAGADRKVLVWDVD 132

Query: 167 SGARAAELKDPNEQVLRVLDYSRISRHLLVTAGDDGTVHLWDTTGRNPK---VSWQKQHS 226
            G      +     V  +L +   ++++L++  DD TV +WD   +N +   ++  ++H 
Sbjct: 133 GGFCTHYFRGHKGVVSSILFHPDSNKNILISGSDDATVRVWDLNAKNTEKKCLAIMEKHF 192

Query: 227 APTAGIGFSPSNDKTLASVGLDKKLYTYD 253
           +    I  S  +  TL S G DK +  +D
Sbjct: 193 SAVTSIALS-EDGLTLFSAGRDKVVNLWD 220

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_023537038.10.0e+0094.13protein NEDD1-like [Cucurbita pepo subsp. pepo][more]
XP_022990911.10.0e+0094.13protein NEDD1 [Cucurbita maxima][more]
XP_038886298.10.0e+0094.00protein NEDD1 isoform X1 [Benincasa hispida][more]
XP_023550844.10.0e+0093.63protein NEDD1-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023550845.1 prote... [more]
KAG6602643.10.0e+0093.75Protein NEDD1, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
B3H5K94.1e-25159.41Protein NEDD1 OS=Arabidopsis thaliana OX=3702 GN=NEDD1 PE=2 SV=1[more]
Q3B7M61.7e-4224.41Protein NEDD1 OS=Bos taurus OX=9913 GN=NEDD1 PE=2 SV=1[more]
Q8NHV41.1e-4123.16Protein NEDD1 OS=Homo sapiens OX=9606 GN=NEDD1 PE=1 SV=1[more]
P332151.3e-3924.50Protein NEDD1 OS=Mus musculus OX=10090 GN=Nedd1 PE=1 SV=2[more]
Q8YRI16.2e-1328.03Uncharacterized WD repeat-containing protein alr3466 OS=Nostoc sp. (strain PCC 7... [more]
Match NameE-valueIdentityDescription
A0A6J1JP940.0e+0094.13protein NEDD1 OS=Cucurbita maxima OX=3661 GN=LOC111487660 PE=4 SV=1[more]
A0A6J1FHX70.0e+0093.38protein NEDD1-like OS=Cucurbita moschata OX=3662 GN=LOC111445500 PE=4 SV=1[more]
A0A6J1H7Q00.0e+0093.88protein NEDD1 OS=Cucurbita moschata OX=3662 GN=LOC111461198 PE=4 SV=1[more]
A0A6J1JX780.0e+0093.25protein NEDD1-like OS=Cucurbita maxima OX=3661 GN=LOC111489662 PE=4 SV=1[more]
A0A0A0KA830.0e+0093.50WD_REPEATS_REGION domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G... [more]
Match NameE-valueIdentityDescription
AT5G05970.22.9e-25259.41Transducin/WD40 repeat-like superfamily protein [more]
AT5G05970.14.3e-25159.41Transducin/WD40 repeat-like superfamily protein [more]
AT3G49660.11.7e-1022.31Transducin/WD40 repeat-like superfamily protein [more]
AT1G11160.13.0e-1023.13Transducin/WD40 repeat-like superfamily protein [more]
AT5G16750.13.3e-0922.15Transducin family protein / WD-40 repeat family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 768..795
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 484..522
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 483..529
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 365..407
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 365..395
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 552..572
NoneNo IPR availablePANTHERPTHR45096:SF1PROTEIN NEDD1coord: 1..795
IPR001680WD40 repeatSMARTSM00320WD40_4coord: 32..72
e-value: 0.013
score: 24.7
coord: 74..122
e-value: 0.83
score: 17.5
coord: 125..164
e-value: 0.1
score: 21.7
coord: 167..208
e-value: 0.25
score: 20.4
coord: 254..293
e-value: 1.0
score: 16.9
coord: 212..252
e-value: 1.1
score: 16.9
IPR001680WD40 repeatPFAMPF00400WD40coord: 193..208
e-value: 0.16
score: 12.9
coord: 43..71
e-value: 0.058
score: 14.3
IPR001680WD40 repeatPROSITEPS50082WD_REPEATS_2coord: 194..208
score: 8.904377
IPR015943WD40/YVTN repeat-like-containing domain superfamilyGENE3D2.130.10.10coord: 177..327
e-value: 4.3E-23
score: 83.9
IPR015943WD40/YVTN repeat-like-containing domain superfamilyGENE3D2.130.10.10coord: 2..176
e-value: 7.9E-20
score: 73.4
IPR044621Protein NEDD1PANTHERPTHR45096PROTEIN NEDD1coord: 1..795
IPR036322WD40-repeat-containing domain superfamilySUPERFAMILY50978WD40 repeat-likecoord: 14..319

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0030312.1Lag0030312.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0000919 cell plate assembly
biological_process GO:0009553 embryo sac development
biological_process GO:0009555 pollen development
biological_process GO:0032467 positive regulation of cytokinesis
biological_process GO:0010968 regulation of microtubule nucleation
biological_process GO:0060236 regulation of mitotic spindle organization
biological_process GO:2000694 regulation of phragmoplast microtubule organization
cellular_component GO:0005828 kinetochore microtubule
molecular_function GO:0140496 gamma-tubulin complex binding
molecular_function GO:0005515 protein binding