Lag0030216 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0030216 vs. NCBI nr
Match: XP_038889573.1 (protein NETWORKED 4A-like [Benincasa hispida] >XP_038889574.1 protein NETWORKED 4A-like [Benincasa hispida])

HSP 1 Score: 807.0 bits (2083), Expect = 9.8e-230
Identity = 446/530 (84.15%), Postives = 479/530 (90.38%), Query Frame = 0

Query: 1   MENPELKKLRPFWAEADIQNRSKWLSNNLEEMNRSVEQMLKAIKENSDNFPKSADMDSQI 60
           ME+PELKKL+PFW EADIQNR KWLSN+LE+MNRSVEQMLK IKENSDNFPKSADMDSQ+
Sbjct: 1   MESPELKKLKPFWPEADIQNRFKWLSNSLEDMNRSVEQMLKTIKENSDNFPKSADMDSQV 60

Query: 61  EEFSRLYQSLVENVLSPELQLQVPVYSDCGSPQGTPELSSDQKQGFNMSSNRGLDISFDS 120
           EEFSRLYQSLVENVLSPELQLQVPVYSDCGSPQGTPELSSDQKQGFN++SNRGLDISFDS
Sbjct: 61  EEFSRLYQSLVENVLSPELQLQVPVYSDCGSPQGTPELSSDQKQGFNLTSNRGLDISFDS 120

Query: 121 GGGSSSLSLKDGTESSSSSSDSESESLNSSVDNNYAVSRAQRDGQGLKKKLLELETELPP 180
           GGGSSSLSLKDGTESS SSSDSESES NSSVDNNY VSR++RD  GLKKKLL +ETEL P
Sbjct: 121 GGGSSSLSLKDGTESSPSSSDSESESFNSSVDNNYVVSRSERDSHGLKKKLLGIETEL-P 180

Query: 181 NMKASLWVDEED--KVNYDELHDKITRIEEELKVSNTKLQSSENEVARLKNELEKNETTI 240
           N K   WV EED  KVNYDELHDKI +IEEELKVSN KLQSSENEV+RLK+ELEKNET I
Sbjct: 181 NSKRGFWVGEEDNHKVNYDELHDKIAKIEEELKVSNAKLQSSENEVSRLKSELEKNETAI 240

Query: 241 LLLEGLQAQLESAEKDNQEREADLQVEKRRVVGLQQQIKQLETRALESDSKTERLIEELE 300
            L EGLQAQLE AEKD QE EADLQV+KR       QI+QLETRAL+SDSK ERLI+ELE
Sbjct: 241 WLSEGLQAQLELAEKDKQEMEADLQVKKR-------QIEQLETRALQSDSKIERLIQELE 300

Query: 301 MSREMLKSSDDKITTLTHELEITKSDHHLQIKELETAFQVSQERFHAEKEQMQTDILRQV 360
            S+EMLKSS DK+T LTHELEITKSDHHLQ+KELETAFQVSQERFHAEKEQMQTDILRQV
Sbjct: 301 TSKEMLKSSSDKVTRLTHELEITKSDHHLQVKELETAFQVSQERFHAEKEQMQTDILRQV 360

Query: 361 EAEKTEMRALHNAHETALQGEIIQLKEELLVRSEGLAALNRNHDELKLKHDMLMAGKDEM 420
           EAEKTE +ALHN+H T LQGEI QLKEEL V+S+GLAALN+NHDELKLK+DMLMA KDE 
Sbjct: 361 EAEKTETKALHNSHLTVLQGEISQLKEELYVKSQGLAALNQNHDELKLKYDMLMAEKDEA 420

Query: 421 SAMVDTLLADKESRESQVHELEGHLQNLKVEKESLIAGSESQNKQIDDLKLRLSELQEEV 480
           SAMV++LLADKESRES VHELEG LQ+L+VEK +LI GSE +NKQIDDLKLRL EL+EEV
Sbjct: 421 SAMVNSLLADKESRESHVHELEGDLQHLQVEKMNLIEGSERKNKQIDDLKLRLLELEEEV 480

Query: 481 NMQKAMIEDGAEGKREAIRQLCFSLEHYRSGYQELREAFIGHKPRPVLSA 529
           N QK MIEDGAEGKREAIRQLCFSLEHYRSGYQELREAFIGHKPRPVLSA
Sbjct: 481 NKQKTMIEDGAEGKREAIRQLCFSLEHYRSGYQELREAFIGHKPRPVLSA 522

BLAST of Lag0030216 vs. NCBI nr
Match: XP_022989923.1 (protein NETWORKED 4A-like [Cucurbita maxima] >XP_022989924.1 protein NETWORKED 4A-like [Cucurbita maxima])

HSP 1 Score: 791.2 bits (2042), Expect = 5.5e-225
Identity = 430/528 (81.44%), Postives = 477/528 (90.34%), Query Frame = 0

Query: 1   MENPELKKLRPFWAEADIQNRSKWLSNNLEEMNRSVEQMLKAIKENSDNFPKSADMDSQI 60
           MEN ELKKL+P W E +IQNRSKWLSNNLEEM R+VEQMLKA+KENSDNFPKSADMDSQ+
Sbjct: 1   MENSELKKLKPLWPEDNIQNRSKWLSNNLEEMTRNVEQMLKAMKENSDNFPKSADMDSQV 60

Query: 61  EEFSRLYQSLVENVLSPELQLQVPVYSDCGSPQGTPELSSDQKQGFNMSSNRGLDISFDS 120
           EEFSRLYQSLVENVLSPELQLQVPVYSDCGSP+GTPELS +QKQ FNMSSNRGLD+SFDS
Sbjct: 61  EEFSRLYQSLVENVLSPELQLQVPVYSDCGSPRGTPELSFNQKQDFNMSSNRGLDVSFDS 120

Query: 121 GGGSSSLSLKDGTESSSSSSDSESESLNSSVDNNYAVSRAQRDGQGLKKKLLELETELPP 180
           GGGSSSLSLKDG+ESSSSSSDSE E    SVDN+YAVSR QRDGQG KKKLLEL+TELPP
Sbjct: 121 GGGSSSLSLKDGSESSSSSSDSELE----SVDNHYAVSRDQRDGQGQKKKLLELQTELPP 180

Query: 181 NMKASLWVDEEDKVNYDELHDKITRIEEELKVSNTKLQSSENEVARLKNELEKNETTILL 240
            ++ +LW DEEDKVNYDEL+++ITR+EEELKVSN KLQSSENEVARLK+E+EKNET ILL
Sbjct: 181 KIRGALWGDEEDKVNYDELYERITRLEEELKVSNIKLQSSENEVARLKSEVEKNETAILL 240

Query: 241 LEGLQAQLESAEKDNQEREADLQVEKRRVVGLQQQIKQLETRALESDSKTERLIEELEMS 300
            EGLQAQLESAEKD Q+ E DLQVEK RV+ L++QI+QLETR+LES+SK ERL EE+E  
Sbjct: 241 SEGLQAQLESAEKDKQQMETDLQVEKGRVLELERQIEQLETRSLESNSKIERLSEEIESC 300

Query: 301 REMLKSSDDKITTLTHELEITKSDHHLQIKELETAFQVSQERFHAEKEQMQTDILRQVEA 360
           +E++KSSDDK T LTHELE TKSDHH QIKELETAFQVSQERFHAEKEQMQTDILR++EA
Sbjct: 301 KEIIKSSDDKTTRLTHELETTKSDHHFQIKELETAFQVSQERFHAEKEQMQTDILRRIEA 360

Query: 361 EKTEMRALHNAHETALQGEIIQLKEELLVRSEGLAALNRNHDELKLKHDMLMAGKDEMSA 420
           +KTEMRALH+AHETALQ EI QLKEEL  RSE LAALNR HDELKLK+DMLMA KD+MSA
Sbjct: 361 DKTEMRALHSAHETALQSEISQLKEELFSRSESLAALNRTHDELKLKYDMLMAEKDDMSA 420

Query: 421 MVDTLLADKESRESQVHELEGHLQNLKVEKESLIAGSESQNKQIDDLKLRLSELQEEVNM 480
           MV+TLLA+KESR+SQ+ ELE HLQNL+ EKE LIAG ESQNKQIDDLK +LS LQEEV +
Sbjct: 421 MVNTLLAEKESRKSQIQELEDHLQNLQAEKEGLIAGFESQNKQIDDLKSKLSALQEEVKI 480

Query: 481 QKAMIEDGAEGKREAIRQLCFSLEHYRSGYQELREAFIGHKPRPVLSA 529
           QK M+EDGA+GK+EAIRQLCFSLEHYRSGYQELREA IGHKPRP LSA
Sbjct: 481 QKTMLEDGAKGKKEAIRQLCFSLEHYRSGYQELREALIGHKPRPALSA 524

BLAST of Lag0030216 vs. NCBI nr
Match: KAG7033248.1 (Protein NETWORKED 4A [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 786.6 bits (2030), Expect = 1.4e-223
Identity = 432/529 (81.66%), Postives = 475/529 (89.79%), Query Frame = 0

Query: 1   MENPELKKLRPFWAEADIQNRSKWLSNNLEEMNRSVEQMLKAIKENSDNFPKSADMDSQI 60
           MEN ELKKL+P W E DIQNRSKWLSNNLEEM R+VEQMLKA+KENSDNFPKSADMDSQ+
Sbjct: 1   MENSELKKLKPLWPEDDIQNRSKWLSNNLEEMTRNVEQMLKAMKENSDNFPKSADMDSQV 60

Query: 61  EEFSRLYQSLVENVLSPELQLQVPVYSDCGSPQGTPELSSDQKQGFNMSSNRGLDISFDS 120
           EEFSRLYQSLV NVLSPELQLQVPVYSDCGSPQGTPELS +QKQ FNMSSNRGLD+SFDS
Sbjct: 61  EEFSRLYQSLVGNVLSPELQLQVPVYSDCGSPQGTPELSLNQKQDFNMSSNRGLDVSFDS 120

Query: 121 GGGSSSLSLKDGTE-SSSSSSDSESESLNSSVDNNYAVSRAQRDGQGLKKKLLELETELP 180
           GGGSSSLSLKDG+E SSSSSS S S+S   SVDN YAVSR QRDGQG KKKLLEL+TELP
Sbjct: 121 GGGSSSLSLKDGSESSSSSSSSSSSDSELESVDNPYAVSRDQRDGQGQKKKLLELQTELP 180

Query: 181 PNMKASLWVDEEDKVNYDELHDKITRIEEELKVSNTKLQSSENEVARLKNELEKNETTIL 240
             ++ +LW DEEDKVNYDEL+++ITR+EEELKVSN KLQSSENEVARLK+E+EKNET IL
Sbjct: 181 SKIRGALWGDEEDKVNYDELYERITRLEEELKVSNIKLQSSENEVARLKSEVEKNETAIL 240

Query: 241 LLEGLQAQLESAEKDNQEREADLQVEKRRVVGLQQQIKQLETRALESDSKTERLIEELEM 300
           L EGLQAQLESAEKD Q+ E DLQVEKRRV+ L++QI+QLETR+LES+SK ERL EE+E 
Sbjct: 241 LSEGLQAQLESAEKDKQQMETDLQVEKRRVLELERQIEQLETRSLESNSKIERLSEEIET 300

Query: 301 SREMLKSSDDKITTLTHELEITKSDHHLQIKELETAFQVSQERFHAEKEQMQTDILRQVE 360
            +E++KSSDDK T LTHELE TKSDHH QIKELETAFQVSQERFH+EKEQMQ DILRQVE
Sbjct: 301 CKEIIKSSDDKTTRLTHELETTKSDHHFQIKELETAFQVSQERFHSEKEQMQIDILRQVE 360

Query: 361 AEKTEMRALHNAHETALQGEIIQLKEELLVRSEGLAALNRNHDELKLKHDMLMAGKDEMS 420
           A+KTEMRALHNAHETALQ EI QLKEEL  R+E LAALNR HDELKLK+DMLMA KD++S
Sbjct: 361 ADKTEMRALHNAHETALQSEISQLKEELFSRNESLAALNRTHDELKLKYDMLMAEKDDIS 420

Query: 421 AMVDTLLADKESRESQVHELEGHLQNLKVEKESLIAGSESQNKQIDDLKLRLSELQEEVN 480
           AMV+TLLADKESR+SQV ELE HLQ L+ EKE LIA SESQNKQIDDLK +LSELQEEV 
Sbjct: 421 AMVNTLLADKESRKSQVQELEDHLQILQAEKEGLIARSESQNKQIDDLKSKLSELQEEVK 480

Query: 481 MQKAMIEDGAEGKREAIRQLCFSLEHYRSGYQELREAFIGHKPRPVLSA 529
           +QK M+EDGA+GK+EAIRQLCFSLEHYRSGYQELREAFIGHKPRP LSA
Sbjct: 481 IQKTMLEDGAKGKKEAIRQLCFSLEHYRSGYQELREAFIGHKPRPALSA 529

BLAST of Lag0030216 vs. NCBI nr
Match: XP_023553367.1 (protein NETWORKED 4A-like [Cucurbita pepo subsp. pepo] >XP_023553438.1 protein NETWORKED 4A-like [Cucurbita pepo subsp. pepo] >XP_023553521.1 protein NETWORKED 4A-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 785.8 bits (2028), Expect = 2.3e-223
Identity = 431/528 (81.63%), Postives = 475/528 (89.96%), Query Frame = 0

Query: 1   MENPELKKLRPFWAEADIQNRSKWLSNNLEEMNRSVEQMLKAIKENSDNFPKSADMDSQI 60
           MEN ELKKL+P W E DIQNRSKWLSNNLEEM R+VEQMLKA+KENSDNFPKSADMDSQ+
Sbjct: 1   MENSELKKLKPLWPEDDIQNRSKWLSNNLEEMTRNVEQMLKAMKENSDNFPKSADMDSQV 60

Query: 61  EEFSRLYQSLVENVLSPELQLQVPVYSDCGSPQGTPELSSDQKQGFNMSSNRGLDISFDS 120
           EEFSRLYQSLVENVLSPELQLQVPVYSDCGSP+GTPELS +QKQ FNMSSNRGLD+SFDS
Sbjct: 61  EEFSRLYQSLVENVLSPELQLQVPVYSDCGSPRGTPELSLNQKQDFNMSSNRGLDVSFDS 120

Query: 121 GGGSSSLSLKDGTESSSSSSDSESESLNSSVDNNYAVSRAQRDGQGLKKKLLELETELPP 180
           GGGSSSLSLKDG+ESSSSSS S+SE    SVDN+YAVSR QRDGQG KKKLLEL+TELP 
Sbjct: 121 GGGSSSLSLKDGSESSSSSSSSDSEL--ESVDNHYAVSRDQRDGQGQKKKLLELQTELPS 180

Query: 181 NMKASLWVDEEDKVNYDELHDKITRIEEELKVSNTKLQSSENEVARLKNELEKNETTILL 240
            ++ +LW DEEDKVNYDEL+++ITR+EEELKVSN KLQSSENEVARLK+E+EKNET ILL
Sbjct: 181 KIRGALWGDEEDKVNYDELYERITRLEEELKVSNIKLQSSENEVARLKSEVEKNETAILL 240

Query: 241 LEGLQAQLESAEKDNQEREADLQVEKRRVVGLQQQIKQLETRALESDSKTERLIEELEMS 300
            EGLQAQLESAEKD Q+ E DLQVEKRRV+ L++QI+QLET +LES+SK ERL  E+E  
Sbjct: 241 SEGLQAQLESAEKDKQQMETDLQVEKRRVLELERQIEQLETWSLESNSKIERLSGEIETC 300

Query: 301 REMLKSSDDKITTLTHELEITKSDHHLQIKELETAFQVSQERFHAEKEQMQTDILRQVEA 360
           +E++KSSDDK T LTHELE TKSDHH +IKELETAFQVSQERFHAEKEQMQ DILRQVEA
Sbjct: 301 KEIIKSSDDKTTRLTHELETTKSDHHFRIKELETAFQVSQERFHAEKEQMQIDILRQVEA 360

Query: 361 EKTEMRALHNAHETALQGEIIQLKEELLVRSEGLAALNRNHDELKLKHDMLMAGKDEMSA 420
           +KTEMRALHNAHETALQ EI QLKEEL  RSE LAALNR HDELKLK+DMLMA KD++SA
Sbjct: 361 DKTEMRALHNAHETALQNEISQLKEELFSRSESLAALNRTHDELKLKYDMLMAEKDDISA 420

Query: 421 MVDTLLADKESRESQVHELEGHLQNLKVEKESLIAGSESQNKQIDDLKLRLSELQEEVNM 480
           MV+TLLADKESR+SQVHELE HLQ L+ EKE LIA SESQNKQIDDLK +LSELQEEV +
Sbjct: 421 MVNTLLADKESRKSQVHELEDHLQILQAEKEGLIARSESQNKQIDDLKSKLSELQEEVKI 480

Query: 481 QKAMIEDGAEGKREAIRQLCFSLEHYRSGYQELREAFIGHKPRPVLSA 529
           QK M+EDGA+GK+EAIRQLCFSLEHYRSGYQELREAFI HKPRP LSA
Sbjct: 481 QKTMLEDGAKGKKEAIRQLCFSLEHYRSGYQELREAFIEHKPRPALSA 526

BLAST of Lag0030216 vs. NCBI nr
Match: XP_022134362.1 (protein NETWORKED 4B [Momordica charantia])

HSP 1 Score: 783.9 bits (2023), Expect = 8.8e-223
Identity = 437/529 (82.61%), Postives = 470/529 (88.85%), Query Frame = 0

Query: 1   MENPELKKLRPFWAEADIQNRSKWLSNNLEEMNRSVEQMLKAIKENSDNFPKSADMDSQI 60
           MENPEL KL PF AEAD QNRSKWLSNNL+ MNRSVEQMLKAIKENS NFPK+ADMDSQ+
Sbjct: 1   MENPELNKLSPFSAEADFQNRSKWLSNNLDGMNRSVEQMLKAIKENSHNFPKTADMDSQV 60

Query: 61  EEFSRLYQSLVENVLSPELQLQVPVYSDCGSPQGTPELSSDQKQGFNMSSNRGLDISFDS 120
           EEFSRLYQ LVE VLSPELQLQVPVYSDCGSPQGTP+LSSDQKQGFNMSSNRGLDISFDS
Sbjct: 61  EEFSRLYQLLVEKVLSPELQLQVPVYSDCGSPQGTPDLSSDQKQGFNMSSNRGLDISFDS 120

Query: 121 GGGSSSLSLKDGTE-SSSSSSDSESESLNSSVDNNYAVSRAQRDGQGLKKKLLELETELP 180
           GGGSSSLSLKDGTE SSSSSSDSESES NSSVDNNYAVSRA++DGQGLKKKLLELETE  
Sbjct: 121 GGGSSSLSLKDGTEFSSSSSSDSESESFNSSVDNNYAVSRAEKDGQGLKKKLLELETE-R 180

Query: 181 PNMKASLWVDEEDKVNYDELHDKITRIEEELKVSNTKLQSSENEVARLKNELEKNETTIL 240
           PNMK + WV EEDKVNYDEL DK  RIEEEL VSN KLQ SE EVARLK++LEKNET  L
Sbjct: 181 PNMKGAFWVGEEDKVNYDELQDKFARIEEELNVSNIKLQLSEKEVARLKSKLEKNETIFL 240

Query: 241 LLEGLQAQLESAEKDNQEREADLQVEKRRVVGLQQQIKQLETRALESDSKTERLIEELEM 300
           L EGLQAQL SAEKD +EREADLQVEK RV+ LQQQ +QL+TRA ESDS+ ERLIEELEM
Sbjct: 241 LSEGLQAQLASAEKDKEEREADLQVEKMRVLELQQQTEQLKTRASESDSRIERLIEELEM 300

Query: 301 SREMLKSSDDKITTLTHELEITKSDHHLQIKELETAFQVSQERFHAEKEQMQTDILRQVE 360
           SREML++SDD+I  LTHEL+I +SDHHLQIKELET FQVSQERFHAEKEQMQ+DIL+QVE
Sbjct: 301 SREMLRNSDDRIAKLTHELDIVQSDHHLQIKELETVFQVSQERFHAEKEQMQSDILKQVE 360

Query: 361 AEKTEMRALHNAHETALQGEIIQLKEELLVRSEGLAALNRNHDELKLKHDMLMAGKDEMS 420
           AEKT  RALH AHETALQGEI QLK+EL  RSE LAALNR HDELKLKHD LMA KDE++
Sbjct: 361 AEKTGTRALHIAHETALQGEISQLKDELSARSESLAALNRYHDELKLKHDTLMAEKDELT 420

Query: 421 AMVDTLLADKESRESQVHELEGHLQNLKVEKESLIAGSESQNKQIDDLKLRLSELQEEVN 480
           A V  LLAD+ESRES+VHELE HLQ+L+VEKE LI+GSES  KQ DDLKL LSELQEEVN
Sbjct: 421 ATVHKLLADEESRESRVHELEVHLQHLQVEKEGLISGSESLKKQNDDLKLELSELQEEVN 480

Query: 481 MQKAMIEDGAEGKREAIRQLCFSLEHYRSGYQELREAFIGHKPRPVLSA 529
            QK  IEDGAEGKREAIRQLCFSL+HYRSGYQELR+AF+GHK RPVL+A
Sbjct: 481 KQKVTIEDGAEGKREAIRQLCFSLDHYRSGYQELRQAFVGHKQRPVLTA 528

BLAST of Lag0030216 vs. ExPASy Swiss-Prot
Match: Q84VY2 (Protein NETWORKED 4B OS=Arabidopsis thaliana OX=3702 GN=NET4B PE=2 SV=1)

HSP 1 Score: 139.0 bits (349), Expect = 1.5e-31
Identity = 162/538 (30.11%), Postives = 256/538 (47.58%), Query Frame = 0

Query: 12  FWAEADIQNRSKWLSNNLEEMNRSVEQMLKAIKENSDNFPKSADMDSQ--------IEEF 71
           +W   +    SKWL+ NLE+M+  V  MLK I+E++D+F K A M  Q        +EEF
Sbjct: 24  WWDSHNCPKNSKWLAENLEKMDDRVNHMLKLIEEDADSFAKKAQMYFQKRPELIQLVEEF 83

Query: 72  SRLYQSLVENVLSPELQLQVPVYSDCGSPQGTPELSSDQKQGFNMSSNRGLDISFDSGGG 131
            R+Y++L E       +LQ    S+  S Q + E+SS  K+                   
Sbjct: 84  YRMYRALAERYDQASGELQKNHTSEIQS-QSSLEISSPTKEKL----------------- 143

Query: 132 SSSLSLKDGTESSSSSSDSESESLNSSVDNNYAVSRAQRDG-QGLKKKLLELETEL-PPN 191
           S   S     E SSS +DS S+S +SS ++         DG + L +++ ELE EL    
Sbjct: 144 SRRQSSHKEEEDSSSLTDSGSDSDHSSAND--------EDGDEALIRRMAELELELQETK 203

Query: 192 MKASLWVDEEDKVNYDELHDKITRIEEELKVSNTKLQSSENEVARLKNELEKNETTILLL 251
            K  L  +  D  N  +L  KIT  E ELK +N K++  E+E+A LKN+L+ +  +    
Sbjct: 204 QKLLLQQESVDGDNNVDLLHKITTYEGELKEANEKMRMHEDEIANLKNQLQ-SFMSFDTE 263

Query: 252 EGLQAQLESAEKDNQEREADLQVEKRRVVGLQQQIKQLETRALESDSKTERLIEELEMSR 311
           + L A+ +S + D ++ + D    K  V+ L++++   + +    + +T  L  ELE+  
Sbjct: 264 DHLGAEQKSVDLDKEDTKEDAVATK--VLALEEELSIAKEKLQHFEKETYSLKNELEIG- 323

Query: 312 EMLKSSDDKITTLTHELEITKSDHHLQIKELETAFQ---VSQERFHAEKEQMQTDILRQV 371
              K++++K+ +L HELE+ + D    I +L    +     QER    K  +Q D   ++
Sbjct: 324 ---KAAEEKLKSLQHELELAQRDADTYINKLNAEKKEVLKLQERLAMVKTSLQ-DRDNEI 383

Query: 372 EAEKTEMRALHN---AHETALQGEIIQLKEELLVRSEGLAALNRN----HDELKLKHDML 431
            A KT +          +  ++GE+ ++ EE     E L  L  +     +E     + L
Sbjct: 384 RALKTAVSDAEQKIFPEKAQIKGEMSKMLEERSQLGEQLRELESHIRLIKEEKAETEEKL 443

Query: 432 MAGKDEMSAMVD---TLLADKESRESQVHELEGHLQNLKVEKESLIAGSESQNKQIDDLK 491
             G +++S M D    L  +   RE ++ E E H++ L +E+                L+
Sbjct: 444 RGGTEKISGMRDESNVLREEIGKREEKIKETEKHMEELHMEQVR--------------LR 503

Query: 492 LRLSELQEEVNMQKAMIEDGAEGKREAIRQLCFSLEHYRSGYQELREAFIGHKPRPVL 527
            R SEL EEV   +    + AE KREAIRQLC SL+HYR GY  L     GHK + V+
Sbjct: 504 RRSSELTEEVERTRVSASEMAEQKREAIRQLCMSLDHYRDGYDRLWRVVAGHKSKRVV 513

BLAST of Lag0030216 vs. ExPASy Swiss-Prot
Match: F4KEW8 (Protein NETWORKED 4A OS=Arabidopsis thaliana OX=3702 GN=NET4A PE=2 SV=1)

HSP 1 Score: 134.0 bits (336), Expect = 4.8e-30
Identity = 178/573 (31.06%), Postives = 282/573 (49.21%), Query Frame = 0

Query: 5   ELKKLRPFWAEADI-QNRSKWLSNNLEEMNRSVEQMLKAIKENSDNFPKSADMDSQ---- 64
           E  K  P+W ++ I    SKWL NNL+EM+RSV++M+K I+E++D+F K A+M  Q    
Sbjct: 16  ESTKSNPWWWDSHIGLKNSKWLENNLDEMDRSVKRMVKLIEEDADSFAKKAEMYYQSRPE 75

Query: 65  ----IEEFSRLYQSLV---ENVL------SP-ELQLQVPVYSDCGSPQGTPELSSDQ--K 124
               ++EF R+Y++L    EN+       SP ELQ Q    SD  +   +   +S++  +
Sbjct: 76  LIALVDEFHRMYRALAERYENITGELRKGSPLELQSQGSGLSDISASDLSALWTSNEVNR 135

Query: 125 QGFNMSSNR--GLDISFDSGGGSSSLSLKDGTESSSSSSDSESESLNSSVDNNYAVSRAQ 184
            G   S  R  G +    +GG  S L  KDG + S+S +DSE ES +SSV N        
Sbjct: 136 LGRPPSGRRAPGFEYFLGNGGLPSDLYHKDG-DDSASITDSELESDDSSVTNYPGYVSIG 195

Query: 185 RDGQGLKKKLLELETELPPNMK----------ASLWVDEEDKVNYDELHDKITRIEEELK 244
            D Q L K++++LE EL    +           SL    + +  + +   K+   E+ELK
Sbjct: 196 SDFQSLSKRIMDLEIELREAKERLRMQLEGNTESLLPRVKSETKFVDFPAKLAACEQELK 255

Query: 245 VSNTKLQSSENEVARLKNELEKNETTILLLEGLQAQLESAEKDNQEREADLQVEKRRVVG 304
             N KLQ+SE+++  LK++L +      L  GL  +        QE + +   E+ R+  
Sbjct: 256 DVNEKLQNSEDQIYILKSQLAR-----YLPSGLDDEQSEGAASTQELDIETLSEELRITS 315

Query: 305 L-------QQQIKQLETRALES-DSKTERLIEELEMSRE-----MLKSSDDK--ITTLTH 364
           L       Q  I + E    +S D+K + L + LE +++       K+S DK  +  L  
Sbjct: 316 LRLREAEKQNGIMRKEVEKSKSDDAKLKSLQDMLESAQKEAAAWKSKASADKREVVKLLD 375

Query: 365 ELEITKS-----DHHLQIKELETAFQVSQERFHAEKEQMQTDILRQVEAEKTEMRALHNA 424
            + + KS     DH  +I++L+TA   ++E+   EK Q++ DI + +E +      +H  
Sbjct: 376 RISMLKSSLAGRDH--EIRDLKTALSDAEEKIFPEKAQVKADIAKLLEEK------IHRD 435

Query: 425 HE-TALQGEIIQLKEE-LLVRSEGLAALNRNHDELKLKHDMLMAGKDEMSAMVDTLLADK 484
            +   L+  +  L++E   V +E +       ++LK + ++L   K E    ++TL    
Sbjct: 436 DQFKELEANVRYLEDERRKVNNEKI----EEEEKLKSEIEVLTLEKVEKGRCIETL---- 495

Query: 485 ESRESQVHELEGHLQNLKVEKESLIAGSESQNKQIDDLKLRLSELQEEVNMQKAMIEDGA 523
                +V ELE  +  L         GSE   K  DD   R  E+++EV  Q+  +E+ A
Sbjct: 496 ---SRKVSELESEISRL---------GSEI--KARDD---RTMEMEKEVEKQRRELEEVA 549

BLAST of Lag0030216 vs. ExPASy Swiss-Prot
Match: Q9LUI2 (Protein NETWORKED 1A OS=Arabidopsis thaliana OX=3702 GN=NET1A PE=1 SV=1)

HSP 1 Score: 50.4 bits (119), Expect = 7.0e-05
Identity = 140/590 (23.73%), Postives = 255/590 (43.22%), Query Frame = 0

Query: 5   ELKKLRPFWAEADIQNRSKWLSNNLEEMNRSVEQMLKAIKENSDNFPKSADMDSQ----- 64
           E ++L  +W ++ I   SKW+  NL +M+  V+ M+K I+E++D+F + A+M  +     
Sbjct: 8   ESRRLYSWWWDSHIPKNSKWIQQNLSDMDSKVKAMIKLIEEDADSFARRAEMYYKKRPEL 67

Query: 65  ---IEEFSRLYQSLVENVLSPELQL-------------QVP-------VYSDCGSPQGTP 124
              +EEF R Y++L E      ++L             QVP         S C  P+ TP
Sbjct: 68  MKLVEEFYRAYRALAERYDHATVELCHAHKTMAEAFPNQVPFDMIEDSASSSCSEPR-TP 127

Query: 125 ELSSDQKQGF----NMSSNRGLDISFDSGGGSSSLSLKDGTESSSSSSDSESESLNSSVD 184
           E      Q F    + +S RGL    +  G S   +  +  + +     +E E+LN    
Sbjct: 128 EKMPPGIQPFYDSDSATSKRGLSQLTEYLGNSE--TEVESLKRTLVELGAEKEALNLQYQ 187

Query: 185 ---NNYA-----VSRAQRDGQGLKKKL--LELETELPPNMKASLWVDEEDK-VNYDELHD 244
              N ++     +  AQ+D  GL ++    E+ET++     A L  + +   + Y+E   
Sbjct: 188 LSLNKFSRLEKDLEVAQKDVSGLDERASKAEIETKILAEALAKLEAERDAALLRYNESMQ 247

Query: 245 KITRIEEELKVSNTKLQSSENEVARLKNELEKNETTILLLEGLQAQLESAEKDNQEREAD 304
           KIT +EE           ++ +V  L N   K ET +  L+   ++L S      E+EA 
Sbjct: 248 KITELEE-------SFSHAQEDVKGLTNRATKAETEVENLKQAHSRLHS------EKEAG 307

Query: 305 LQVEKRRVVGLQQQIKQLETRALESDSKTERLIEELEMSREMLKSSDDKITTLTHEL-EI 364
           L    R +    + I  LE +  +++   +        S +  K ++D+I  L HEL ++
Sbjct: 308 LAEYNRCL----EMISNLEKKVRDAEENAQ------NFSNQSAK-AEDEIKALRHELVKV 367

Query: 365 TKSDHHLQIKE---LETAFQVSQERFHAE--KEQMQTDILRQVEAEKT---EMRALHNAH 424
            +    L+++    LET  ++ +E  HA+   +++ +++L      KT   +   L +++
Sbjct: 368 NEVKDGLRLRYQQCLETISKLEREVSHAQDNAKRLSSEVLAGAAKLKTVEDQCTLLESSN 427

Query: 425 ET-------------ALQGEIIQLKEEL-----LVRSEGLAALNRNHDELKLKHDMLMAG 484
           ET             A   EI Q + EL     L+  E    L      LK    +    
Sbjct: 428 ETLKLEADGLTHKLAAKDQEIFQKQNELEKFQSLIEDEHSRYL-EIEVSLKTLQSLYSQS 487

Query: 485 KDEMSAMVDTL------LADKESRESQVHELEGHLQNLKVEKESLIAGSESQNKQIDDLK 516
           ++E   +   L      L D E+R     +LEG + ++K E ++L   ++S    ++  K
Sbjct: 488 QEEQKVITSELQSRIGMLRDLETRNL---KLEGDISSVKEENQNLSELNDSSMIFLETQK 547

BLAST of Lag0030216 vs. ExPASy TrEMBL
Match: A0A6J1JQP9 (protein NETWORKED 4A-like OS=Cucurbita maxima OX=3661 GN=LOC111486963 PE=4 SV=1)

HSP 1 Score: 791.2 bits (2042), Expect = 2.7e-225
Identity = 430/528 (81.44%), Postives = 477/528 (90.34%), Query Frame = 0

Query: 1   MENPELKKLRPFWAEADIQNRSKWLSNNLEEMNRSVEQMLKAIKENSDNFPKSADMDSQI 60
           MEN ELKKL+P W E +IQNRSKWLSNNLEEM R+VEQMLKA+KENSDNFPKSADMDSQ+
Sbjct: 1   MENSELKKLKPLWPEDNIQNRSKWLSNNLEEMTRNVEQMLKAMKENSDNFPKSADMDSQV 60

Query: 61  EEFSRLYQSLVENVLSPELQLQVPVYSDCGSPQGTPELSSDQKQGFNMSSNRGLDISFDS 120
           EEFSRLYQSLVENVLSPELQLQVPVYSDCGSP+GTPELS +QKQ FNMSSNRGLD+SFDS
Sbjct: 61  EEFSRLYQSLVENVLSPELQLQVPVYSDCGSPRGTPELSFNQKQDFNMSSNRGLDVSFDS 120

Query: 121 GGGSSSLSLKDGTESSSSSSDSESESLNSSVDNNYAVSRAQRDGQGLKKKLLELETELPP 180
           GGGSSSLSLKDG+ESSSSSSDSE E    SVDN+YAVSR QRDGQG KKKLLEL+TELPP
Sbjct: 121 GGGSSSLSLKDGSESSSSSSDSELE----SVDNHYAVSRDQRDGQGQKKKLLELQTELPP 180

Query: 181 NMKASLWVDEEDKVNYDELHDKITRIEEELKVSNTKLQSSENEVARLKNELEKNETTILL 240
            ++ +LW DEEDKVNYDEL+++ITR+EEELKVSN KLQSSENEVARLK+E+EKNET ILL
Sbjct: 181 KIRGALWGDEEDKVNYDELYERITRLEEELKVSNIKLQSSENEVARLKSEVEKNETAILL 240

Query: 241 LEGLQAQLESAEKDNQEREADLQVEKRRVVGLQQQIKQLETRALESDSKTERLIEELEMS 300
            EGLQAQLESAEKD Q+ E DLQVEK RV+ L++QI+QLETR+LES+SK ERL EE+E  
Sbjct: 241 SEGLQAQLESAEKDKQQMETDLQVEKGRVLELERQIEQLETRSLESNSKIERLSEEIESC 300

Query: 301 REMLKSSDDKITTLTHELEITKSDHHLQIKELETAFQVSQERFHAEKEQMQTDILRQVEA 360
           +E++KSSDDK T LTHELE TKSDHH QIKELETAFQVSQERFHAEKEQMQTDILR++EA
Sbjct: 301 KEIIKSSDDKTTRLTHELETTKSDHHFQIKELETAFQVSQERFHAEKEQMQTDILRRIEA 360

Query: 361 EKTEMRALHNAHETALQGEIIQLKEELLVRSEGLAALNRNHDELKLKHDMLMAGKDEMSA 420
           +KTEMRALH+AHETALQ EI QLKEEL  RSE LAALNR HDELKLK+DMLMA KD+MSA
Sbjct: 361 DKTEMRALHSAHETALQSEISQLKEELFSRSESLAALNRTHDELKLKYDMLMAEKDDMSA 420

Query: 421 MVDTLLADKESRESQVHELEGHLQNLKVEKESLIAGSESQNKQIDDLKLRLSELQEEVNM 480
           MV+TLLA+KESR+SQ+ ELE HLQNL+ EKE LIAG ESQNKQIDDLK +LS LQEEV +
Sbjct: 421 MVNTLLAEKESRKSQIQELEDHLQNLQAEKEGLIAGFESQNKQIDDLKSKLSALQEEVKI 480

Query: 481 QKAMIEDGAEGKREAIRQLCFSLEHYRSGYQELREAFIGHKPRPVLSA 529
           QK M+EDGA+GK+EAIRQLCFSLEHYRSGYQELREA IGHKPRP LSA
Sbjct: 481 QKTMLEDGAKGKKEAIRQLCFSLEHYRSGYQELREALIGHKPRPALSA 524

BLAST of Lag0030216 vs. ExPASy TrEMBL
Match: A0A6J1BZF1 (protein NETWORKED 4B OS=Momordica charantia OX=3673 GN=LOC111006642 PE=4 SV=1)

HSP 1 Score: 783.9 bits (2023), Expect = 4.3e-223
Identity = 437/529 (82.61%), Postives = 470/529 (88.85%), Query Frame = 0

Query: 1   MENPELKKLRPFWAEADIQNRSKWLSNNLEEMNRSVEQMLKAIKENSDNFPKSADMDSQI 60
           MENPEL KL PF AEAD QNRSKWLSNNL+ MNRSVEQMLKAIKENS NFPK+ADMDSQ+
Sbjct: 1   MENPELNKLSPFSAEADFQNRSKWLSNNLDGMNRSVEQMLKAIKENSHNFPKTADMDSQV 60

Query: 61  EEFSRLYQSLVENVLSPELQLQVPVYSDCGSPQGTPELSSDQKQGFNMSSNRGLDISFDS 120
           EEFSRLYQ LVE VLSPELQLQVPVYSDCGSPQGTP+LSSDQKQGFNMSSNRGLDISFDS
Sbjct: 61  EEFSRLYQLLVEKVLSPELQLQVPVYSDCGSPQGTPDLSSDQKQGFNMSSNRGLDISFDS 120

Query: 121 GGGSSSLSLKDGTE-SSSSSSDSESESLNSSVDNNYAVSRAQRDGQGLKKKLLELETELP 180
           GGGSSSLSLKDGTE SSSSSSDSESES NSSVDNNYAVSRA++DGQGLKKKLLELETE  
Sbjct: 121 GGGSSSLSLKDGTEFSSSSSSDSESESFNSSVDNNYAVSRAEKDGQGLKKKLLELETE-R 180

Query: 181 PNMKASLWVDEEDKVNYDELHDKITRIEEELKVSNTKLQSSENEVARLKNELEKNETTIL 240
           PNMK + WV EEDKVNYDEL DK  RIEEEL VSN KLQ SE EVARLK++LEKNET  L
Sbjct: 181 PNMKGAFWVGEEDKVNYDELQDKFARIEEELNVSNIKLQLSEKEVARLKSKLEKNETIFL 240

Query: 241 LLEGLQAQLESAEKDNQEREADLQVEKRRVVGLQQQIKQLETRALESDSKTERLIEELEM 300
           L EGLQAQL SAEKD +EREADLQVEK RV+ LQQQ +QL+TRA ESDS+ ERLIEELEM
Sbjct: 241 LSEGLQAQLASAEKDKEEREADLQVEKMRVLELQQQTEQLKTRASESDSRIERLIEELEM 300

Query: 301 SREMLKSSDDKITTLTHELEITKSDHHLQIKELETAFQVSQERFHAEKEQMQTDILRQVE 360
           SREML++SDD+I  LTHEL+I +SDHHLQIKELET FQVSQERFHAEKEQMQ+DIL+QVE
Sbjct: 301 SREMLRNSDDRIAKLTHELDIVQSDHHLQIKELETVFQVSQERFHAEKEQMQSDILKQVE 360

Query: 361 AEKTEMRALHNAHETALQGEIIQLKEELLVRSEGLAALNRNHDELKLKHDMLMAGKDEMS 420
           AEKT  RALH AHETALQGEI QLK+EL  RSE LAALNR HDELKLKHD LMA KDE++
Sbjct: 361 AEKTGTRALHIAHETALQGEISQLKDELSARSESLAALNRYHDELKLKHDTLMAEKDELT 420

Query: 421 AMVDTLLADKESRESQVHELEGHLQNLKVEKESLIAGSESQNKQIDDLKLRLSELQEEVN 480
           A V  LLAD+ESRES+VHELE HLQ+L+VEKE LI+GSES  KQ DDLKL LSELQEEVN
Sbjct: 421 ATVHKLLADEESRESRVHELEVHLQHLQVEKEGLISGSESLKKQNDDLKLELSELQEEVN 480

Query: 481 MQKAMIEDGAEGKREAIRQLCFSLEHYRSGYQELREAFIGHKPRPVLSA 529
            QK  IEDGAEGKREAIRQLCFSL+HYRSGYQELR+AF+GHK RPVL+A
Sbjct: 481 KQKVTIEDGAEGKREAIRQLCFSLDHYRSGYQELRQAFVGHKQRPVLTA 528

BLAST of Lag0030216 vs. ExPASy TrEMBL
Match: A0A6J1JXC2 (protein NETWORKED 4A OS=Cucurbita maxima OX=3661 GN=LOC111489163 PE=4 SV=1)

HSP 1 Score: 783.5 bits (2022), Expect = 5.6e-223
Identity = 434/528 (82.20%), Postives = 473/528 (89.58%), Query Frame = 0

Query: 1   MENPELKKLRPFWAEADIQNRSKWLSNNLEEMNRSVEQMLKAIKENSDNFPKSADMDSQI 60
           MENPELK L+PFW EADIQNRSKWLS +LEEMNRSVEQMLK IK+NS+NFPKSADMDSQ+
Sbjct: 1   MENPELKNLKPFWPEADIQNRSKWLSISLEEMNRSVEQMLKTIKDNSNNFPKSADMDSQV 60

Query: 61  EEFSRLYQSLVENVLSPELQLQVPVYSDCGSPQGTPELSSDQKQGFNMSSNRGLDISFDS 120
           EEFSRLYQSL+EN+LSPELQ QVP YSDCGSPQ TPELSSDQKQGFN+S NRGLDIS DS
Sbjct: 61  EEFSRLYQSLLENLLSPELQPQVPFYSDCGSPQCTPELSSDQKQGFNLSCNRGLDISLDS 120

Query: 121 GGGSSSLSLKDGTESSSSSSDSESESLNSSVDNNYAVSRAQRDGQGLKKKLLELETELPP 180
           GGGSSSLSLKDGT SSSSSSDSESES +SSVDNNY VSRA+RDGQG KKK L LETEL  
Sbjct: 121 GGGSSSLSLKDGTGSSSSSSDSESESFSSSVDNNYVVSRAERDGQGRKKKFLGLETEL-S 180

Query: 181 NMKASLWVDEEDKVNYDELHDKITRIEEELKVSNTKLQSSENEVARLKNELEKNETTILL 240
           N+K + WV EEDK+NYDELH+KI RIEEELKVSN KLQSSENEVARLK+ELEKNET + L
Sbjct: 181 NIKGAFWVGEEDKLNYDELHEKIARIEEELKVSNAKLQSSENEVARLKSELEKNETAMSL 240

Query: 241 LEGLQAQLESAEKDNQEREADLQVEKRRVVGLQQQIKQLETRALESDSKTERLIEELEMS 300
            EGLQAQLESAEKD QE  ADLQVEKRRVV L+QQI+QLETR L+SDSK E LIEELEMS
Sbjct: 241 SEGLQAQLESAEKDKQEMGADLQVEKRRVVELEQQIEQLETRVLQSDSKIEILIEELEMS 300

Query: 301 REMLKSSDDKITTLTHELEITKSDHHLQIKELETAFQVSQERFHAEKEQMQTDILRQVEA 360
           RE+LKSSDDKI  LTHELEITKSDH LQIKELE+AFQVSQE F+AEKEQMQ D+LRQVEA
Sbjct: 301 REILKSSDDKIARLTHELEITKSDHRLQIKELESAFQVSQEIFNAEKEQMQADVLRQVEA 360

Query: 361 EKTEMRALHNAHETALQGEIIQLKEELLVRSEGLAALNRNHDELKLKHDMLMAGKDEMSA 420
           +KTE+RALHN+H T+LQGEI QLKEEL V+S+ L+ALN NHDELKLK++MLMA KDE +A
Sbjct: 361 DKTEIRALHNSHLTSLQGEISQLKEELFVKSQSLSALNSNHDELKLKYNMLMAEKDEATA 420

Query: 421 MVDTLLADKESRESQVHELEGHLQNLKVEKESLIAGSESQNKQIDDLKLRLSELQEEVNM 480
           MV TL+ADKESRES VHELE HLQ+L+ E+E LIAGSE QNKQIDDLKLRLSE+QEEVN 
Sbjct: 421 MVHTLVADKESRESHVHELESHLQHLQEEEEGLIAGSEIQNKQIDDLKLRLSEMQEEVNR 480

Query: 481 QKAMIEDGAEGKREAIRQLCFSLEHYRSGYQELREAFIGHKPRPVLSA 529
           Q  MIEDGAE KREAIRQLCFSLEHYRSGY+ELREAFIGHKPR VLSA
Sbjct: 481 QMNMIEDGAERKREAIRQLCFSLEHYRSGYKELREAFIGHKPRQVLSA 527

BLAST of Lag0030216 vs. ExPASy TrEMBL
Match: A0A6J1EC39 (protein NETWORKED 4A-like OS=Cucurbita moschata OX=3662 GN=LOC111431199 PE=4 SV=1)

HSP 1 Score: 782.7 bits (2020), Expect = 9.5e-223
Identity = 431/529 (81.47%), Postives = 474/529 (89.60%), Query Frame = 0

Query: 1   MENPELKKLRPFWAEADIQNRSKWLSNNLEEMNRSVEQMLKAIKENSDNFPKSADMDSQI 60
           MEN ELKKL+P W E DIQNRSKWLSNNLEEM R+VEQMLKA+KENSDNFPKSADMDSQ+
Sbjct: 1   MENSELKKLKPLWPEDDIQNRSKWLSNNLEEMTRNVEQMLKAMKENSDNFPKSADMDSQV 60

Query: 61  EEFSRLYQSLVENVLSPELQLQVPVYSDCGSPQGTPELSSDQKQGFNMSSNRGLDISFDS 120
           EEFSRLYQSLV NVLSPELQLQVPVYSDCGSPQGTPELS +QKQ FNMSSNRGLD+SFDS
Sbjct: 61  EEFSRLYQSLVGNVLSPELQLQVPVYSDCGSPQGTPELSLNQKQDFNMSSNRGLDVSFDS 120

Query: 121 GGGSSSLSLKDGTE-SSSSSSDSESESLNSSVDNNYAVSRAQRDGQGLKKKLLELETELP 180
           GGGSSSLSLKDG+E SSSSSS S S+S   SVDN YAVSR QRDGQG KKKLLEL+TELP
Sbjct: 121 GGGSSSLSLKDGSESSSSSSSSSSSDSELESVDNPYAVSRDQRDGQGQKKKLLELQTELP 180

Query: 181 PNMKASLWVDEEDKVNYDELHDKITRIEEELKVSNTKLQSSENEVARLKNELEKNETTIL 240
             ++ +LW DEEDKVNYDEL+++I R+EEELKVSN KLQSSENEVARLK+E+EKNET IL
Sbjct: 181 SKIRGALWGDEEDKVNYDELYERIARLEEELKVSNIKLQSSENEVARLKSEVEKNETAIL 240

Query: 241 LLEGLQAQLESAEKDNQEREADLQVEKRRVVGLQQQIKQLETRALESDSKTERLIEELEM 300
           L EGLQAQLESAEKD Q+ E DL+VEKRRV+ L++QI+QLETR+LES+SK ERL EE+E 
Sbjct: 241 LSEGLQAQLESAEKDKQQMETDLRVEKRRVLELERQIEQLETRSLESNSKIERLSEEIET 300

Query: 301 SREMLKSSDDKITTLTHELEITKSDHHLQIKELETAFQVSQERFHAEKEQMQTDILRQVE 360
            +E++KSSDDK T LTHELE TKS+HH QIKELETAFQVSQERFHAEKEQMQ DILRQVE
Sbjct: 301 CKEIIKSSDDKTTRLTHELETTKSNHHFQIKELETAFQVSQERFHAEKEQMQIDILRQVE 360

Query: 361 AEKTEMRALHNAHETALQGEIIQLKEELLVRSEGLAALNRNHDELKLKHDMLMAGKDEMS 420
           A+KTEMRALHNAHETALQ EI QLKEEL  RSE LAALNR HDELKLK+DMLMA KD++S
Sbjct: 361 ADKTEMRALHNAHETALQREISQLKEELFSRSESLAALNRTHDELKLKYDMLMAEKDDIS 420

Query: 421 AMVDTLLADKESRESQVHELEGHLQNLKVEKESLIAGSESQNKQIDDLKLRLSELQEEVN 480
           AMV+TLLADKESR+SQV ELE HLQ L+ EKE LIA SESQNKQIDDLK +LSELQEEV 
Sbjct: 421 AMVNTLLADKESRKSQVQELEDHLQILQAEKEGLIARSESQNKQIDDLKSKLSELQEEVK 480

Query: 481 MQKAMIEDGAEGKREAIRQLCFSLEHYRSGYQELREAFIGHKPRPVLSA 529
           +QK M+EDGA+GK+EAIRQLCFSLEHYRSGYQELREAFIGHKPRP LSA
Sbjct: 481 IQKTMLEDGAKGKKEAIRQLCFSLEHYRSGYQELREAFIGHKPRPALSA 529

BLAST of Lag0030216 vs. ExPASy TrEMBL
Match: A0A6J1FN40 (protein NETWORKED 4A-like OS=Cucurbita moschata OX=3662 GN=LOC111445431 PE=4 SV=1)

HSP 1 Score: 776.9 bits (2005), Expect = 5.2e-221
Identity = 432/527 (81.97%), Postives = 469/527 (88.99%), Query Frame = 0

Query: 1   MENPELKKLRPFWAEADIQNRSKWLSNNLEEMNRSVEQMLKAIKENSDNFPKSADMDSQI 60
           MENPELK L+PFW EADIQNRSKWLS +LEEMNRSVEQMLK IK+N DNFPKSADMDSQ+
Sbjct: 1   MENPELKNLKPFWPEADIQNRSKWLSVSLEEMNRSVEQMLKTIKDNCDNFPKSADMDSQV 60

Query: 61  EEFSRLYQSLVENVLSPELQLQVPVYSDCGSPQGTPELSSDQKQGFNMSSNRGLDISFDS 120
           EEFSRLYQSL+EN+LSPELQ QVP YSDCGSPQ TPELSSDQKQGFN+S NRGLDIS DS
Sbjct: 61  EEFSRLYQSLLENLLSPELQPQVPFYSDCGSPQCTPELSSDQKQGFNLSCNRGLDISLDS 120

Query: 121 GGGSSSLSLKDGTESSSSSSDSESESLNSSVDNNYAVSRAQRDGQGLKKKLLELETELPP 180
           GGGSSSLSLKDGT SSSSSSDSESES +SSVDNNY VSRA+RDGQG KKK L LETEL  
Sbjct: 121 GGGSSSLSLKDGTGSSSSSSDSESESFSSSVDNNYVVSRAERDGQGPKKKFLGLETEL-S 180

Query: 181 NMKASLWVDEEDKVNYDELHDKITRIEEELKVSNTKLQSSENEVARLKNELEKNETTILL 240
           N++ + WV EEDK+NY ELH+KI RIEEELKVSN KLQSSENEVARLK+ELEKNET + L
Sbjct: 181 NIEGAFWVGEEDKLNYHELHEKIARIEEELKVSNAKLQSSENEVARLKSELEKNETAMSL 240

Query: 241 LEGLQAQLESAEKDNQEREADLQVEKRRVVGLQQQIKQLETRALESDSKTERLIEELEMS 300
            EGLQAQLESAEKD QE  ADLQV KRRVV L+QQI+QLETR L+SDSK E LIEELEMS
Sbjct: 241 SEGLQAQLESAEKDKQEMGADLQVLKRRVVELEQQIEQLETRVLQSDSKIEILIEELEMS 300

Query: 301 REMLKSSDDKITTLTHELEITKSDHHLQIKELETAFQVSQERFHAEKEQMQTDILRQVEA 360
           RE+LKSSDDKI  LTHELEITKSDH LQIKELE+AFQVSQE F+AEKEQ+Q D+LRQVEA
Sbjct: 301 REILKSSDDKIARLTHELEITKSDHRLQIKELESAFQVSQELFNAEKEQVQADVLRQVEA 360

Query: 361 EKTEMRALHNAHETALQGEIIQLKEELLVRSEGLAALNRNHDELKLKHDMLMAGKDEMSA 420
           +KTEMRALHN+H T+LQGEI QLKEELLV+S+ L+ALN NHDELKLK+DMLMA KDE +A
Sbjct: 361 DKTEMRALHNSHLTSLQGEISQLKEELLVKSQSLSALNSNHDELKLKYDMLMAEKDEATA 420

Query: 421 MVDTLLADKESRESQVHELEGHLQNLKVEKESLIAGSESQNKQIDDLKLRLSELQEEVNM 480
           MV TL+ADKE RES VHELE HLQ+L+ EKE LIAGSESQNKQIDDLKL LSE+QEEVN 
Sbjct: 421 MVHTLVADKEYRESHVHELESHLQHLQEEKEGLIAGSESQNKQIDDLKLGLSEMQEEVNR 480

Query: 481 QKAMIEDGAEGKREAIRQLCFSLEHYRSGYQELREAFIGHKPRPVLS 528
           Q  MIEDGAE KREAIRQLCFSLEHYRSGY+ELREAFIGHKPR VLS
Sbjct: 481 QMNMIEDGAERKREAIRQLCFSLEHYRSGYKELREAFIGHKPRQVLS 526

BLAST of Lag0030216 vs. TAIR 10
Match: AT5G05180.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT3G10880.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink). )

HSP 1 Score: 154.5 bits (389), Expect = 2.4e-37
Identity = 139/429 (32.40%), Postives = 222/429 (51.75%), Query Frame = 0

Query: 109 SSNRGLDISFDSGGGSSSLSLKDGTESSSSSSDSESESLNSSVDNNYAVSRAQRDGQGLK 168
           +SN  +DIS D  G S  ++ K  +++ +S+ D  S  ++ + D+N + S    D +   
Sbjct: 15  NSNSFVDISTDHNGSSPPVTPK--SDTVASNFDQGSSDVSGNHDSNSSYSDLDSDTEAFY 74

Query: 169 KKLLELETELPPNMKASLWVDEEDKVNYDELHDKITRIEEELKVSNTKLQSSENEVARLK 228
                    + P    S  +  E +++Y+EL  K  + EEEL+ ++ KLQ  E E+ +LK
Sbjct: 75  SSF--NHHLVSPGSMDSHDLSPEKQMSYEELMKKYVQCEEELRTTSLKLQEFEQEIEKLK 134

Query: 229 NELEKNETTILLLEGLQAQLESAEKDNQEREADLQVEKRRVVGLQQQIKQLETRALESDS 288
            E EK E+ +L  E L+ + E A+ +   R+  ++ E++RV+ +Q+Q+  LET   +   
Sbjct: 135 -ETEKKESVVLFGEYLRGEREIAQGEIAIRDIAIETERKRVLEVQRQVVDLETELSDLSF 194

Query: 289 KTERLIEELEMSREMLKSSDDKITTLTHELEITKSDHHLQIKELETAFQVSQERFHAEKE 348
           K E L+ E E+SR+ L  S  +I+ L   L                     Q+ F  EK 
Sbjct: 195 KFEHLVNEHEVSRDCLDVSFSEISKLREML------------------CDCQQNFSIEKT 254

Query: 349 QMQTDILRQVEAEKTEMRALHNAHETALQGEIIQLKEELLVRSEGLAALNRNHDELKLKH 408
           ++  D ++  EAEK EM+      E  LQ EI  LK +L  R E + ALN++ D+ KL++
Sbjct: 255 KL-VDQIKHSEAEKMEMQ----RKEVELQAEISALKTDLATRGEHIEALNKDFDKHKLRY 314

Query: 409 DMLMAGKDEMSAMVDTLLADKESRESQVHELEGHLQNLKVEKESLIAGSESQNKQIDDLK 468
           DMLMA KD + A VD L A+  SR+ Q+ ++E  L  L  ++  L++ S +    +++LK
Sbjct: 315 DMLMAEKDGVCAEVDNLKAEMRSRDIQIQQMEEQLNQLVYKQTELVSESGNAKNTVEELK 374

Query: 469 LRLSELQEEV------------------------NMQKAMIEDGAEGKREAIRQLCFSLE 514
             + EL+ EV                         +Q+  I  G E KREAIRQLCFSL+
Sbjct: 375 AVVKELEIEVELQSKAKKTVEELRATVWEMEKHAELQRNAISQGEEEKREAIRQLCFSLD 415

BLAST of Lag0030216 vs. TAIR 10
Match: AT5G05180.2 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT3G10880.1); Has 50151 Blast hits to 28081 proteins in 1838 species: Archae - 860; Bacteria - 6013; Metazoa - 25867; Fungi - 3836; Plants - 2236; Viruses - 90; Other Eukaryotes - 11249 (source: NCBI BLink). )

HSP 1 Score: 139.8 bits (351), Expect = 6.2e-33
Identity = 132/429 (30.77%), Postives = 211/429 (49.18%), Query Frame = 0

Query: 109 SSNRGLDISFDSGGGSSSLSLKDGTESSSSSSDSESESLNSSVDNNYAVSRAQRDGQGLK 168
           +SN  +DIS D  G S  ++ K  +++ +S+ D  S  ++ + D+N + S    D +   
Sbjct: 15  NSNSFVDISTDHNGSSPPVTPK--SDTVASNFDQGSSDVSGNHDSNSSYSDLDSDTEAFY 74

Query: 169 KKLLELETELPPNMKASLWVDEEDKVNYDELHDKITRIEEELKVSNTKLQSSENEVARLK 228
                    + P    S  +  E +++Y+EL  K  + EEEL+ ++ KLQ  E E+ +LK
Sbjct: 75  SSF--NHHLVSPGSMDSHDLSPEKQMSYEELMKKYVQCEEELRTTSLKLQEFEQEIEKLK 134

Query: 229 NELEKNETTILLLEGLQAQLESAEKDNQEREADLQVEKRRVVGLQQQIKQLETRALESDS 288
            E EK E+ +L  E L+ + E A+ +   R+  ++ E++RV+ +Q+Q+  LET   +   
Sbjct: 135 -ETEKKESVVLFGEYLRGEREIAQGEIAIRDIAIETERKRVLEVQRQVVDLETELSDLSF 194

Query: 289 KTERLIEELEMSREMLKSSDDKITTLTHELEITKSDHHLQIKELETAFQVSQERFHAEKE 348
           K E L+ E                   HE                       + F  EK 
Sbjct: 195 KFEHLVNE-------------------HE-----------------------QNFSIEKT 254

Query: 349 QMQTDILRQVEAEKTEMRALHNAHETALQGEIIQLKEELLVRSEGLAALNRNHDELKLKH 408
           ++  D ++  EAEK EM+      E  LQ EI  LK +L  R E + ALN++ D+ KL++
Sbjct: 255 KL-VDQIKHSEAEKMEMQ----RKEVELQAEISALKTDLATRGEHIEALNKDFDKHKLRY 314

Query: 409 DMLMAGKDEMSAMVDTLLADKESRESQVHELEGHLQNLKVEKESLIAGSESQNKQIDDLK 468
           DMLMA KD + A VD L A+  SR+ Q+ ++E  L  L  ++  L++ S +    +++LK
Sbjct: 315 DMLMAEKDGVCAEVDNLKAEMRSRDIQIQQMEEQLNQLVYKQTELVSESGNAKNTVEELK 374

Query: 469 LRLSELQEEV------------------------NMQKAMIEDGAEGKREAIRQLCFSLE 514
             + EL+ EV                         +Q+  I  G E KREAIRQLCFSL+
Sbjct: 375 AVVKELEIEVELQSKAKKTVEELRATVWEMEKHAELQRNAISQGEEEKREAIRQLCFSLD 391

BLAST of Lag0030216 vs. TAIR 10
Match: AT2G30500.1 (Kinase interacting (KIP1-like) family protein )

HSP 1 Score: 139.0 bits (349), Expect = 1.1e-32
Identity = 162/538 (30.11%), Postives = 256/538 (47.58%), Query Frame = 0

Query: 12  FWAEADIQNRSKWLSNNLEEMNRSVEQMLKAIKENSDNFPKSADMDSQ--------IEEF 71
           +W   +    SKWL+ NLE+M+  V  MLK I+E++D+F K A M  Q        +EEF
Sbjct: 24  WWDSHNCPKNSKWLAENLEKMDDRVNHMLKLIEEDADSFAKKAQMYFQKRPELIQLVEEF 83

Query: 72  SRLYQSLVENVLSPELQLQVPVYSDCGSPQGTPELSSDQKQGFNMSSNRGLDISFDSGGG 131
            R+Y++L E       +LQ    S+  S Q + E+SS  K+                   
Sbjct: 84  YRMYRALAERYDQASGELQKNHTSEIQS-QSSLEISSPTKEKL----------------- 143

Query: 132 SSSLSLKDGTESSSSSSDSESESLNSSVDNNYAVSRAQRDG-QGLKKKLLELETEL-PPN 191
           S   S     E SSS +DS S+S +SS ++         DG + L +++ ELE EL    
Sbjct: 144 SRRQSSHKEEEDSSSLTDSGSDSDHSSAND--------EDGDEALIRRMAELELELQETK 203

Query: 192 MKASLWVDEEDKVNYDELHDKITRIEEELKVSNTKLQSSENEVARLKNELEKNETTILLL 251
            K  L  +  D  N  +L  KIT  E ELK +N K++  E+E+A LKN+L+ +  +    
Sbjct: 204 QKLLLQQESVDGDNNVDLLHKITTYEGELKEANEKMRMHEDEIANLKNQLQ-SFMSFDTE 263

Query: 252 EGLQAQLESAEKDNQEREADLQVEKRRVVGLQQQIKQLETRALESDSKTERLIEELEMSR 311
           + L A+ +S + D ++ + D    K  V+ L++++   + +    + +T  L  ELE+  
Sbjct: 264 DHLGAEQKSVDLDKEDTKEDAVATK--VLALEEELSIAKEKLQHFEKETYSLKNELEIG- 323

Query: 312 EMLKSSDDKITTLTHELEITKSDHHLQIKELETAFQ---VSQERFHAEKEQMQTDILRQV 371
              K++++K+ +L HELE+ + D    I +L    +     QER    K  +Q D   ++
Sbjct: 324 ---KAAEEKLKSLQHELELAQRDADTYINKLNAEKKEVLKLQERLAMVKTSLQ-DRDNEI 383

Query: 372 EAEKTEMRALHN---AHETALQGEIIQLKEELLVRSEGLAALNRN----HDELKLKHDML 431
            A KT +          +  ++GE+ ++ EE     E L  L  +     +E     + L
Sbjct: 384 RALKTAVSDAEQKIFPEKAQIKGEMSKMLEERSQLGEQLRELESHIRLIKEEKAETEEKL 443

Query: 432 MAGKDEMSAMVD---TLLADKESRESQVHELEGHLQNLKVEKESLIAGSESQNKQIDDLK 491
             G +++S M D    L  +   RE ++ E E H++ L +E+                L+
Sbjct: 444 RGGTEKISGMRDESNVLREEIGKREEKIKETEKHMEELHMEQVR--------------LR 503

Query: 492 LRLSELQEEVNMQKAMIEDGAEGKREAIRQLCFSLEHYRSGYQELREAFIGHKPRPVL 527
            R SEL EEV   +    + AE KREAIRQLC SL+HYR GY  L     GHK + V+
Sbjct: 504 RRSSELTEEVERTRVSASEMAEQKREAIRQLCMSLDHYRDGYDRLWRVVAGHKSKRVV 513

BLAST of Lag0030216 vs. TAIR 10
Match: AT2G30500.2 (Kinase interacting (KIP1-like) family protein )

HSP 1 Score: 139.0 bits (349), Expect = 1.1e-32
Identity = 162/538 (30.11%), Postives = 256/538 (47.58%), Query Frame = 0

Query: 12  FWAEADIQNRSKWLSNNLEEMNRSVEQMLKAIKENSDNFPKSADMDSQ--------IEEF 71
           +W   +    SKWL+ NLE+M+  V  MLK I+E++D+F K A M  Q        +EEF
Sbjct: 24  WWDSHNCPKNSKWLAENLEKMDDRVNHMLKLIEEDADSFAKKAQMYFQKRPELIQLVEEF 83

Query: 72  SRLYQSLVENVLSPELQLQVPVYSDCGSPQGTPELSSDQKQGFNMSSNRGLDISFDSGGG 131
            R+Y++L E       +LQ    S+  S Q + E+SS  K+                   
Sbjct: 84  YRMYRALAERYDQASGELQKNHTSEIQS-QSSLEISSPTKEKL----------------- 143

Query: 132 SSSLSLKDGTESSSSSSDSESESLNSSVDNNYAVSRAQRDG-QGLKKKLLELETEL-PPN 191
           S   S     E SSS +DS S+S +SS ++         DG + L +++ ELE EL    
Sbjct: 144 SRRQSSHKEEEDSSSLTDSGSDSDHSSAND--------EDGDEALIRRMAELELELQETK 203

Query: 192 MKASLWVDEEDKVNYDELHDKITRIEEELKVSNTKLQSSENEVARLKNELEKNETTILLL 251
            K  L  +  D  N  +L  KIT  E ELK +N K++  E+E+A LKN+L+ +  +    
Sbjct: 204 QKLLLQQESVDGDNNVDLLHKITTYEGELKEANEKMRMHEDEIANLKNQLQ-SFMSFDTE 263

Query: 252 EGLQAQLESAEKDNQEREADLQVEKRRVVGLQQQIKQLETRALESDSKTERLIEELEMSR 311
           + L A+ +S + D ++ + D    K  V+ L++++   + +    + +T  L  ELE+  
Sbjct: 264 DHLGAEQKSVDLDKEDTKEDAVATK--VLALEEELSIAKEKLQHFEKETYSLKNELEIG- 323

Query: 312 EMLKSSDDKITTLTHELEITKSDHHLQIKELETAFQ---VSQERFHAEKEQMQTDILRQV 371
              K++++K+ +L HELE+ + D    I +L    +     QER    K  +Q D   ++
Sbjct: 324 ---KAAEEKLKSLQHELELAQRDADTYINKLNAEKKEVLKLQERLAMVKTSLQ-DRDNEI 383

Query: 372 EAEKTEMRALHN---AHETALQGEIIQLKEELLVRSEGLAALNRN----HDELKLKHDML 431
            A KT +          +  ++GE+ ++ EE     E L  L  +     +E     + L
Sbjct: 384 RALKTAVSDAEQKIFPEKAQIKGEMSKMLEERSQLGEQLRELESHIRLIKEEKAETEEKL 443

Query: 432 MAGKDEMSAMVD---TLLADKESRESQVHELEGHLQNLKVEKESLIAGSESQNKQIDDLK 491
             G +++S M D    L  +   RE ++ E E H++ L +E+                L+
Sbjct: 444 RGGTEKISGMRDESNVLREEIGKREEKIKETEKHMEELHMEQVR--------------LR 503

Query: 492 LRLSELQEEVNMQKAMIEDGAEGKREAIRQLCFSLEHYRSGYQELREAFIGHKPRPVL 527
            R SEL EEV   +    + AE KREAIRQLC SL+HYR GY  L     GHK + V+
Sbjct: 504 RRSSELTEEVERTRVSASEMAEQKREAIRQLCMSLDHYRDGYDRLWRVVAGHKSKRVV 513

BLAST of Lag0030216 vs. TAIR 10
Match: AT5G58320.2 (Kinase interacting (KIP1-like) family protein )

HSP 1 Score: 134.0 bits (336), Expect = 3.4e-31
Identity = 178/573 (31.06%), Postives = 282/573 (49.21%), Query Frame = 0

Query: 5   ELKKLRPFWAEADI-QNRSKWLSNNLEEMNRSVEQMLKAIKENSDNFPKSADMDSQ---- 64
           E  K  P+W ++ I    SKWL NNL+EM+RSV++M+K I+E++D+F K A+M  Q    
Sbjct: 16  ESTKSNPWWWDSHIGLKNSKWLENNLDEMDRSVKRMVKLIEEDADSFAKKAEMYYQSRPE 75

Query: 65  ----IEEFSRLYQSLV---ENVL------SP-ELQLQVPVYSDCGSPQGTPELSSDQ--K 124
               ++EF R+Y++L    EN+       SP ELQ Q    SD  +   +   +S++  +
Sbjct: 76  LIALVDEFHRMYRALAERYENITGELRKGSPLELQSQGSGLSDISASDLSALWTSNEVNR 135

Query: 125 QGFNMSSNR--GLDISFDSGGGSSSLSLKDGTESSSSSSDSESESLNSSVDNNYAVSRAQ 184
            G   S  R  G +    +GG  S L  KDG + S+S +DSE ES +SSV N        
Sbjct: 136 LGRPPSGRRAPGFEYFLGNGGLPSDLYHKDG-DDSASITDSELESDDSSVTNYPGYVSIG 195

Query: 185 RDGQGLKKKLLELETELPPNMK----------ASLWVDEEDKVNYDELHDKITRIEEELK 244
            D Q L K++++LE EL    +           SL    + +  + +   K+   E+ELK
Sbjct: 196 SDFQSLSKRIMDLEIELREAKERLRMQLEGNTESLLPRVKSETKFVDFPAKLAACEQELK 255

Query: 245 VSNTKLQSSENEVARLKNELEKNETTILLLEGLQAQLESAEKDNQEREADLQVEKRRVVG 304
             N KLQ+SE+++  LK++L +      L  GL  +        QE + +   E+ R+  
Sbjct: 256 DVNEKLQNSEDQIYILKSQLAR-----YLPSGLDDEQSEGAASTQELDIETLSEELRITS 315

Query: 305 L-------QQQIKQLETRALES-DSKTERLIEELEMSRE-----MLKSSDDK--ITTLTH 364
           L       Q  I + E    +S D+K + L + LE +++       K+S DK  +  L  
Sbjct: 316 LRLREAEKQNGIMRKEVEKSKSDDAKLKSLQDMLESAQKEAAAWKSKASADKREVVKLLD 375

Query: 365 ELEITKS-----DHHLQIKELETAFQVSQERFHAEKEQMQTDILRQVEAEKTEMRALHNA 424
            + + KS     DH  +I++L+TA   ++E+   EK Q++ DI + +E +      +H  
Sbjct: 376 RISMLKSSLAGRDH--EIRDLKTALSDAEEKIFPEKAQVKADIAKLLEEK------IHRD 435

Query: 425 HE-TALQGEIIQLKEE-LLVRSEGLAALNRNHDELKLKHDMLMAGKDEMSAMVDTLLADK 484
            +   L+  +  L++E   V +E +       ++LK + ++L   K E    ++TL    
Sbjct: 436 DQFKELEANVRYLEDERRKVNNEKI----EEEEKLKSEIEVLTLEKVEKGRCIETL---- 495

Query: 485 ESRESQVHELEGHLQNLKVEKESLIAGSESQNKQIDDLKLRLSELQEEVNMQKAMIEDGA 523
                +V ELE  +  L         GSE   K  DD   R  E+++EV  Q+  +E+ A
Sbjct: 496 ---SRKVSELESEISRL---------GSEI--KARDD---RTMEMEKEVEKQRRELEEVA 549