Lag0030208 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0030208 vs. NCBI nr
Match: XP_038890905.1 (cytokinin hydroxylase-like [Benincasa hispida])

HSP 1 Score: 977.2 bits (2525), Expect = 5.4e-281
Identity = 486/515 (94.37%), Postives = 496/515 (96.31%), Query Frame = 0

Query: 8   VVLTTLALAAATSFFIFSLLYHTLSSYCLTPRRIKNKMAKQGVRGPKPRPLLGNILDVAA 67
           ++LTTL L   TSFFIFSLLY TLS YC TPRRIK+KMAKQGVRGPKPRPLLGNILDVAA
Sbjct: 7   LLLTTLVL-LTTSFFIFSLLYDTLSCYCFTPRRIKHKMAKQGVRGPKPRPLLGNILDVAA 66

Query: 68  LLSKSTSSDMPSISHDLVPRLLPHFLAWSAQFGKRFIFWNGIEPRMCLTETDLIKELLSK 127
           LLSKSTSSDMPSISHDLVPRLLPHFLAWS QFGKRFIFWNGIEPRMCLTETDLIKELLSK
Sbjct: 67  LLSKSTSSDMPSISHDLVPRLLPHFLAWSTQFGKRFIFWNGIEPRMCLTETDLIKELLSK 126

Query: 128 YSAVSGKSWLQQQGSKHFIGRGLLMANGQNWFHQRHIVAPAFVGDRLKSYAGYMVECTRE 187
           YSAVSGKSWLQQQGSKHFIGRGLLMANGQNWFHQRHIVAPAFVGDRLKSYAGYMVECTRE
Sbjct: 127 YSAVSGKSWLQQQGSKHFIGRGLLMANGQNWFHQRHIVAPAFVGDRLKSYAGYMVECTRE 186

Query: 188 MLQSIENEVKSGRSEFEIGEYMTRLTADIISRTEFESSYEKGKQIFHLLTVLQHLCAQAS 247
           ML+SIENEVKSGRSEFEIGEYMTRLTADIISRTEFESSYEKGKQIFHLLTVLQHLCAQAS
Sbjct: 187 MLESIENEVKSGRSEFEIGEYMTRLTADIISRTEFESSYEKGKQIFHLLTVLQHLCAQAS 246

Query: 248 RHLCLPGSRFFPSKYNREIKALKGKVEGLLMEIIQSRRDCVEIGRSSSYGNDLLGMLLNE 307
           RHLCLPGSRFFPSKYNREIKALKGKVE LLMEIIQSRRDCVEIGRSSSYGNDLLGMLLNE
Sbjct: 247 RHLCLPGSRFFPSKYNREIKALKGKVEELLMEIIQSRRDCVEIGRSSSYGNDLLGMLLNE 306

Query: 308 MQKKKDGNGLSLNLQIIMDECKTFFFAGHETTALLLTWTVMLLATNPSWQQKVRAEVMTV 367
           MQKKKDGNGLSLNLQIIMDECKTFFFAGHETTALLLTWTVMLLATNPSWQ K+RAEV+ +
Sbjct: 307 MQKKKDGNGLSLNLQIIMDECKTFFFAGHETTALLLTWTVMLLATNPSWQHKLRAEVIAI 366

Query: 368 CNGQTPSLDHLSKLSLLNMVINESLRLYPPASILPRMAFEDIKLGDLEIPKGLSIWIPVL 427
           C  +TPS  HLSKLSLL MVINESLRLYPPASILPRMAFEDIKLGDLEIPKGLSIWIPVL
Sbjct: 367 CGSETPSFHHLSKLSLLGMVINESLRLYPPASILPRMAFEDIKLGDLEIPKGLSIWIPVL 426

Query: 428 AIHHSEELWGKDVNEFNPERFSNSKAFTSGRFIPFASGPRNCVGQSFALMETKIILAMLI 487
           AIHHSEE+WGKD NEFNPERF+NSKAFTSG FIPFASGPRNCVGQSFALMETKIILAMLI
Sbjct: 427 AIHHSEEIWGKDANEFNPERFANSKAFTSGGFIPFASGPRNCVGQSFALMETKIILAMLI 486

Query: 488 SKFSFTISDNYRHAPVVVLTIKPKYGVQVCLKPLN 523
           SKFSFTISD YRHAPVVVLTIKPKYGVQVCLKPLN
Sbjct: 487 SKFSFTISDKYRHAPVVVLTIKPKYGVQVCLKPLN 520

BLAST of Lag0030208 vs. NCBI nr
Match: XP_011656036.1 (cytokinin hydroxylase [Cucumis sativus] >KAE8648965.1 hypothetical protein Csa_008948 [Cucumis sativus])

HSP 1 Score: 957.2 bits (2473), Expect = 5.8e-275
Identity = 482/518 (93.05%), Postives = 495/518 (95.56%), Query Frame = 0

Query: 7   LVVLTTLALAAATSFFIFSLLYHTLSSYCLTPRRIKNKMAKQGVRGPKPRPLLGNILDVA 66
           L +LTTLAL   TSFFIFSLLY TLS Y  TPRRIK KMAKQGV GPKPRPLLGNILDVA
Sbjct: 6   LPLLTTLAL-LTTSFFIFSLLYDTLSCYLFTPRRIKQKMAKQGVHGPKPRPLLGNILDVA 65

Query: 67  ALLSKSTSSDMPSISHDLVPRLLPHFLAWSAQFGKRFIFWNGIEPRMCLTETDLIKELLS 126
           +LLSKSTSSDMPSISHDLVPRLLPHFLAWS+QFGKRFIFWNGIEPRMCLTETDLIKELLS
Sbjct: 66  SLLSKSTSSDMPSISHDLVPRLLPHFLAWSSQFGKRFIFWNGIEPRMCLTETDLIKELLS 125

Query: 127 KYSAVSGKSWLQQQGSKHFIGRGLLMANGQNWFHQRHIVAPAFVGDRLKSYAGYMVECTR 186
           KYSAVSGKSWLQQQGSKHFIGRGLLMANGQNWFHQRHIVAPAFVGDRLKSYAGYMVECT+
Sbjct: 126 KYSAVSGKSWLQQQGSKHFIGRGLLMANGQNWFHQRHIVAPAFVGDRLKSYAGYMVECTK 185

Query: 187 EMLQSIENEVKSGRSEFEIGEYMTRLTADIISRTEFESSYEKGKQIFHLLTVLQHLCAQA 246
           EML+S+E EVKSGRSEFEIGEYMTRLTADIISRTEFESS+EKGKQIFHLLTVLQHLCAQA
Sbjct: 186 EMLESLEKEVKSGRSEFEIGEYMTRLTADIISRTEFESSFEKGKQIFHLLTVLQHLCAQA 245

Query: 247 SRHLCLPGSRFFPSKYNREIKALKGKVEGLLMEIIQSRRDCVEIGRSSSYGNDLLGMLLN 306
           SRHLCLPGSRFFPSKYNREIKALKGKVE LLMEIIQSRRDCVEIGRSSSYGNDLLGMLLN
Sbjct: 246 SRHLCLPGSRFFPSKYNREIKALKGKVEELLMEIIQSRRDCVEIGRSSSYGNDLLGMLLN 305

Query: 307 EMQKKK-DG-NGLSLNLQIIMDECKTFFFAGHETTALLLTWTVMLLATNPSWQQKVRAEV 366
           EMQKKK DG NGLSLNLQIIMDECKTFFFAGHETTALLLTWTVMLLATNP+WQ KVRAEV
Sbjct: 306 EMQKKKLDGNNGLSLNLQIIMDECKTFFFAGHETTALLLTWTVMLLATNPTWQNKVRAEV 365

Query: 367 MTVCNGQTPSLDHLSKLSLLNMVINESLRLYPPASILPRMAFEDIKLGDLEIPKGLSIWI 426
           M VC  +TPS  HLSKLSLL+MVINESLRLYPPASILPRMAFEDIKLGDLEIPKGLSIWI
Sbjct: 366 MAVCGSETPSFHHLSKLSLLSMVINESLRLYPPASILPRMAFEDIKLGDLEIPKGLSIWI 425

Query: 427 PVLAIHHSEELWGKDVNEFNPERFSNSKAFTSGRFIPFASGPRNCVGQSFALMETKIILA 486
           PVLAIHHSEELWGKD NEFNPERF+NSK FTSG FIPFASGPRNCVGQSFALMETKIILA
Sbjct: 426 PVLAIHHSEELWGKDANEFNPERFANSKPFTSGGFIPFASGPRNCVGQSFALMETKIILA 485

Query: 487 MLISKFSFTISDNYRHAPVVVLTIKPKYGVQVCLKPLN 523
           MLISKFSFTISD+YRHAPVVVLTIKPKYGVQVCL+PLN
Sbjct: 486 MLISKFSFTISDSYRHAPVVVLTIKPKYGVQVCLEPLN 522

BLAST of Lag0030208 vs. NCBI nr
Match: KAA0039372.1 (cytokinin hydroxylase-like [Cucumis melo var. makuwa])

HSP 1 Score: 956.8 bits (2472), Expect = 7.6e-275
Identity = 480/517 (92.84%), Postives = 494/517 (95.55%), Query Frame = 0

Query: 8   VVLTTLALAAATSFFIFSLLYHTLSSYCLTPRRIKNKMAKQGVRGPKPRPLLGNILDVAA 67
           ++LTTLAL   TSFFIFSLLY T+S Y LTPRRIK KMAKQGV GPKPRPLLGNILDVA+
Sbjct: 7   LLLTTLAL-LTTSFFIFSLLYDTVSCYLLTPRRIKQKMAKQGVHGPKPRPLLGNILDVAS 66

Query: 68  LLSKSTSSDMPSISHDLVPRLLPHFLAWSAQFGKRFIFWNGIEPRMCLTETDLIKELLSK 127
           LLSKSTSSDMPSISHDLVPRLLPHFLAWS+QFGKRFIFWNGIEPRMCLTETDLIKELLSK
Sbjct: 67  LLSKSTSSDMPSISHDLVPRLLPHFLAWSSQFGKRFIFWNGIEPRMCLTETDLIKELLSK 126

Query: 128 YSAVSGKSWLQQQGSKHFIGRGLLMANGQNWFHQRHIVAPAFVGDRLKSYAGYMVECTRE 187
           YS VSGKSWLQQQGSKHFIGRGLLMANGQNWFHQRHIVAPAFVGDRLKSYAGYMVECT+E
Sbjct: 127 YSTVSGKSWLQQQGSKHFIGRGLLMANGQNWFHQRHIVAPAFVGDRLKSYAGYMVECTKE 186

Query: 188 MLQSIENEVKSGRSEFEIGEYMTRLTADIISRTEFESSYEKGKQIFHLLTVLQHLCAQAS 247
           ML+SIE EVKSGRSEFEIGEYMTRLTADIISRTEFESS+EKGKQIFHLLTVLQHLCAQAS
Sbjct: 187 MLESIEKEVKSGRSEFEIGEYMTRLTADIISRTEFESSFEKGKQIFHLLTVLQHLCAQAS 246

Query: 248 RHLCLPGSRFFPSKYNREIKALKGKVEGLLMEIIQSRRDCVEIGRSSSYGNDLLGMLLNE 307
           RHLC PGSRFFPSKYNREIKALKGKVE LLMEIIQSR+DCVEIGRSSSYGNDLLGMLLNE
Sbjct: 247 RHLCFPGSRFFPSKYNREIKALKGKVEELLMEIIQSRKDCVEIGRSSSYGNDLLGMLLNE 306

Query: 308 MQKKK-DG-NGLSLNLQIIMDECKTFFFAGHETTALLLTWTVMLLATNPSWQQKVRAEVM 367
           MQKKK DG NGLSLNLQIIMDECKTFFFAGHETTALLLTWTVMLLATNP+WQ KVRAEVM
Sbjct: 307 MQKKKLDGNNGLSLNLQIIMDECKTFFFAGHETTALLLTWTVMLLATNPTWQDKVRAEVM 366

Query: 368 TVCNGQTPSLDHLSKLSLLNMVINESLRLYPPASILPRMAFEDIKLGDLEIPKGLSIWIP 427
            VC  +TPS  HLSKLSLL+MVINESLRLYPPASILPRMAFEDIKLGDLEIPKGLSIWIP
Sbjct: 367 AVCGSETPSFHHLSKLSLLSMVINESLRLYPPASILPRMAFEDIKLGDLEIPKGLSIWIP 426

Query: 428 VLAIHHSEELWGKDVNEFNPERFSNSKAFTSGRFIPFASGPRNCVGQSFALMETKIILAM 487
           VLAIHHSEELWGKD NEFNPERF+NSK FTSG FIPFASGPRNC+GQSFALMETKIILAM
Sbjct: 427 VLAIHHSEELWGKDANEFNPERFANSKPFTSGGFIPFASGPRNCIGQSFALMETKIILAM 486

Query: 488 LISKFSFTISDNYRHAPVVVLTIKPKYGVQVCLKPLN 523
           LISKFSFTISDNYRHAPVVVLTIKPKYGVQVCLKPLN
Sbjct: 487 LISKFSFTISDNYRHAPVVVLTIKPKYGVQVCLKPLN 522

BLAST of Lag0030208 vs. NCBI nr
Match: XP_008459472.1 (PREDICTED: cytokinin hydroxylase-like [Cucumis melo])

HSP 1 Score: 955.7 bits (2469), Expect = 1.7e-274
Identity = 479/517 (92.65%), Postives = 494/517 (95.55%), Query Frame = 0

Query: 8   VVLTTLALAAATSFFIFSLLYHTLSSYCLTPRRIKNKMAKQGVRGPKPRPLLGNILDVAA 67
           ++LTTLAL   TSFFIFSLLY T+S Y LTPRRIK KMAKQGV GPKPRPLLGNILDVA+
Sbjct: 7   LLLTTLAL-LTTSFFIFSLLYDTVSCYLLTPRRIKQKMAKQGVHGPKPRPLLGNILDVAS 66

Query: 68  LLSKSTSSDMPSISHDLVPRLLPHFLAWSAQFGKRFIFWNGIEPRMCLTETDLIKELLSK 127
           LLSKSTSSDMPSISHDLVPRLLPHFLAWS+QFGKRFIFWNGIEPRMCLTETDLIKELLSK
Sbjct: 67  LLSKSTSSDMPSISHDLVPRLLPHFLAWSSQFGKRFIFWNGIEPRMCLTETDLIKELLSK 126

Query: 128 YSAVSGKSWLQQQGSKHFIGRGLLMANGQNWFHQRHIVAPAFVGDRLKSYAGYMVECTRE 187
           YS VSGKSWLQQQGSKHFIGRGLLMANGQNWFHQRHIVAPAFVGDRLKSYAGYMVECT+E
Sbjct: 127 YSTVSGKSWLQQQGSKHFIGRGLLMANGQNWFHQRHIVAPAFVGDRLKSYAGYMVECTKE 186

Query: 188 MLQSIENEVKSGRSEFEIGEYMTRLTADIISRTEFESSYEKGKQIFHLLTVLQHLCAQAS 247
           ML+SIE EVKSGRSEFEIGEYMTRLTADIISRTEFESS+EKGKQIFHLLTVLQHLCAQAS
Sbjct: 187 MLESIEKEVKSGRSEFEIGEYMTRLTADIISRTEFESSFEKGKQIFHLLTVLQHLCAQAS 246

Query: 248 RHLCLPGSRFFPSKYNREIKALKGKVEGLLMEIIQSRRDCVEIGRSSSYGNDLLGMLLNE 307
           RHLC PGSRFFPSKYNREIKALKGKVE LLMEIIQSR+DCVEIGRSSSYGNDLLGMLLNE
Sbjct: 247 RHLCFPGSRFFPSKYNREIKALKGKVEELLMEIIQSRKDCVEIGRSSSYGNDLLGMLLNE 306

Query: 308 MQKKK-DG-NGLSLNLQIIMDECKTFFFAGHETTALLLTWTVMLLATNPSWQQKVRAEVM 367
           MQKKK DG NGLSLNLQIIMDECKTFFFAGHETTALLLTWTVMLLATNP+WQ KVRAEVM
Sbjct: 307 MQKKKLDGNNGLSLNLQIIMDECKTFFFAGHETTALLLTWTVMLLATNPTWQDKVRAEVM 366

Query: 368 TVCNGQTPSLDHLSKLSLLNMVINESLRLYPPASILPRMAFEDIKLGDLEIPKGLSIWIP 427
            VC  +TPS  HLSKLSLL+MVINESLRLYPPASILPRMAFEDIKLGDLEIPKGLSIWIP
Sbjct: 367 AVCGSETPSFHHLSKLSLLSMVINESLRLYPPASILPRMAFEDIKLGDLEIPKGLSIWIP 426

Query: 428 VLAIHHSEELWGKDVNEFNPERFSNSKAFTSGRFIPFASGPRNCVGQSFALMETKIILAM 487
           +LAIHHSEELWGKD NEFNPERF+NSK FTSG FIPFASGPRNC+GQSFALMETKIILAM
Sbjct: 427 LLAIHHSEELWGKDANEFNPERFANSKPFTSGGFIPFASGPRNCIGQSFALMETKIILAM 486

Query: 488 LISKFSFTISDNYRHAPVVVLTIKPKYGVQVCLKPLN 523
           LISKFSFTISDNYRHAPVVVLTIKPKYGVQVCLKPLN
Sbjct: 487 LISKFSFTISDNYRHAPVVVLTIKPKYGVQVCLKPLN 522

BLAST of Lag0030208 vs. NCBI nr
Match: XP_022939611.1 (cytokinin hydroxylase-like isoform X1 [Cucurbita moschata] >KAG7016062.1 Cytokinin hydroxylase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 950.3 bits (2455), Expect = 7.1e-273
Identity = 476/521 (91.36%), Postives = 494/521 (94.82%), Query Frame = 0

Query: 1   MAVTFALVVLTTLALAAATSFFIFSLLYHTLSSYCLTPRRIKNKMAKQGVRGPKPRPLLG 60
           MA+  AL +LTTLA  AATSF IFSLLYHTLS Y LTPRRI  KMAKQGVRGPKPRPLLG
Sbjct: 1   MAIMLAL-LLTTLAF-AATSFCIFSLLYHTLSCYWLTPRRIMRKMAKQGVRGPKPRPLLG 60

Query: 61  NILDVAALLSKSTSSDMPSISHDLVPRLLPHFLAWSAQFGKRFIFWNGIEPRMCLTETDL 120
           NILDVAALLSKSTSSDMPSISHDLVPRLLPH+LAWSAQFGKRFIFWNGIEPRMCLTET L
Sbjct: 61  NILDVAALLSKSTSSDMPSISHDLVPRLLPHYLAWSAQFGKRFIFWNGIEPRMCLTETHL 120

Query: 121 IKELLSKYSAVSGKSWLQQQGSKHFIGRGLLMANGQNWFHQRHIVAPAFVGDRLKSYAGY 180
           IKELLSKYS+VSGKSWLQQQGSKHFIGRGLLMANG NWFHQRHIVAPAFVGDRLKSYAGY
Sbjct: 121 IKELLSKYSSVSGKSWLQQQGSKHFIGRGLLMANGPNWFHQRHIVAPAFVGDRLKSYAGY 180

Query: 181 MVECTREMLQSIENEVKSGRSEFEIGEYMTRLTADIISRTEFESSYEKGKQIFHLLTVLQ 240
           MVECTREMLQS+ENEVKSGRSEFEIGEYMTRLTADIISRTEFESSYEKGKQIFHLLTVLQ
Sbjct: 181 MVECTREMLQSLENEVKSGRSEFEIGEYMTRLTADIISRTEFESSYEKGKQIFHLLTVLQ 240

Query: 241 HLCAQASRHLCLPGSRFFPSKYNREIKALKGKVEGLLMEIIQSRRDCVEIGRSSSYGNDL 300
           HLCAQASRHLCLPGSRFFPSKYNREIKALKGKVEGLLMEIIQSR+D VEIGRSSSYGNDL
Sbjct: 241 HLCAQASRHLCLPGSRFFPSKYNREIKALKGKVEGLLMEIIQSRKDGVEIGRSSSYGNDL 300

Query: 301 LGMLLNEMQKKKDGNGLSLNLQIIMDECKTFFFAGHETTALLLTWTVMLLATNPSWQQKV 360
           LGMLLNEMQKKK+GNGLSLNLQIIMDECKTFFFAGHETTALLLTWT+MLLATNPSWQ +V
Sbjct: 301 LGMLLNEMQKKKNGNGLSLNLQIIMDECKTFFFAGHETTALLLTWTIMLLATNPSWQDRV 360

Query: 361 RAEVMTVCNGQTPSLDHLSKLSLLNMVINESLRLYPPASILPRMAFEDIKLGDLEIPKGL 420
           RAE+  VCN QTPSL HLSKL LL MVINESLRLYPPASILPRMAFEDIKLGDLEIPKGL
Sbjct: 361 RAELNAVCNSQTPSLHHLSKLPLLGMVINESLRLYPPASILPRMAFEDIKLGDLEIPKGL 420

Query: 421 SIWIPVLAIHHSEELWGKDVNEFNPERFSNSKAFTSGRFIPFASGPRNCVGQSFALMETK 480
           S+WIPV+AIHHSEE+WGKD NEFNPERF +SKAFT G FIPFASGPRNCVGQ+FALMETK
Sbjct: 421 SVWIPVVAIHHSEEIWGKDANEFNPERFGSSKAFTCGGFIPFASGPRNCVGQAFALMETK 480

Query: 481 IILAMLISKFSFTISDNYRHAPVVVLTIKPKYGVQVCLKPL 522
           IILAMLISKF FTIS +YRHAPVVVLT+KPKYGVQ+CL PL
Sbjct: 481 IILAMLISKFRFTISPSYRHAPVVVLTVKPKYGVQICLTPL 519

BLAST of Lag0030208 vs. ExPASy Swiss-Prot
Match: Q9FF18 (Cytokinin hydroxylase OS=Arabidopsis thaliana OX=3702 GN=CYP735A1 PE=1 SV=1)

HSP 1 Score: 733.0 bits (1891), Expect = 2.3e-210
Identity = 365/518 (70.46%), Postives = 432/518 (83.40%), Query Frame = 0

Query: 7   LVVLTTLALAAATSFFIFSLLYHTLSSYCLTPRRIKNKMAKQGVRGPKPRPLLGNILDVA 66
           L +L +L +   T+  I  +LY T+S Y LTPRRIK  M +QGV GPKPRPL GNIL+++
Sbjct: 3   LTILKSLLVIFVTT--ILRVLYDTISCYWLTPRRIKKIMEQQGVTGPKPRPLTGNILEIS 62

Query: 67  ALLSKSTSSDMPSISHDLVPRLLPHFLAWSAQFGKRFIFWNGIEPRMCLTETDLIKELLS 126
           A++S+S S D  SI HD+V RLLPH++AWS Q+GKRFI WNG +PR+CLTET+LIKELL 
Sbjct: 63  AMVSQSASKDCDSIHHDIVGRLLPHYVAWSKQYGKRFIVWNGTDPRLCLTETELIKELLM 122

Query: 127 KYSAVSGKSWLQQQGSKHFIGRGLLMANGQNWFHQRHIVAPAFVGDRLKSYAGYMVECTR 186
           K++ VSG+SWLQQQG+K+FIGRGLLMANGQ+W HQRH+ APAF G+RLK YA +MVECT 
Sbjct: 123 KHNGVSGRSWLQQQGTKNFIGRGLLMANGQDWHHQRHLAAPAFTGERLKGYARHMVECTS 182

Query: 187 EMLQSIENEVKSGRSEFEIGEYMTRLTADIISRTEFESSYEKGKQIFHLLTVLQHLCAQA 246
           ++++ +  EV  G +E EIGE M +LTADIISRT+F SS+EKGK++F+ LTVLQ  CAQA
Sbjct: 183 KLVERLRKEVGEGANEVEIGEEMHKLTADIISRTKFGSSFEKGKELFNHLTVLQRRCAQA 242

Query: 247 SRHLCLPGSRFFPSKYNREIKALKGKVEGLLMEIIQSRRDCVEIGRSSSYGNDLLGMLLN 306
           +RHLC PGSRF PSKYNREIK+LK +VE LL+EIIQSRRDC E+GRSS++G+DLLG+LLN
Sbjct: 243 TRHLCFPGSRFLPSKYNREIKSLKKEVERLLIEIIQSRRDCAEMGRSSTHGDDLLGLLLN 302

Query: 307 EMQKKKDGNGLSLNLQIIMDECKTFFFAGHETTALLLTWTVMLLATNPSWQQKVRAEVMT 366
           EM   K+ N  + NLQ+IMDECKTFFFAGHETTALLLTWT MLLA NP+WQ+KVR EV  
Sbjct: 303 EMDIDKNNNNNNNNLQLIMDECKTFFFAGHETTALLLTWTTMLLADNPTWQEKVREEVRE 362

Query: 367 VC--NGQTPSLDHLSKLSLLNMVINESLRLYPPASILPRMAFEDIKLGDLEIPKGLSIWI 426
           V   NG  PS+D LSKL+ L+ VINESLRLYPPA++LPRMAFED+KLGDL IPKGLSIWI
Sbjct: 363 VFGRNG-LPSVDQLSKLTSLSKVINESLRLYPPATLLPRMAFEDLKLGDLTIPKGLSIWI 422

Query: 427 PVLAIHHSEELWGKDVNEFNPERFSNSKAFTSGR-FIPFASGPRNCVGQSFALMETKIIL 486
           PVLAIHHSEELWGKD N+FNPERF   + F SGR FIPFA+GPRNC+GQ FALME KIIL
Sbjct: 423 PVLAIHHSEELWGKDANQFNPERF-GGRPFASGRHFIPFAAGPRNCIGQQFALMEAKIIL 482

Query: 487 AMLISKFSFTISDNYRHAPVVVLTIKPKYGVQVCLKPL 522
           A LISKF+FTIS NYRHAP+VVLTIKPKYGVQV LKPL
Sbjct: 483 ATLISKFNFTISKNYRHAPIVVLTIKPKYGVQVILKPL 516

BLAST of Lag0030208 vs. ExPASy Swiss-Prot
Match: Q9ZW95 (Cytokinin hydroxylase OS=Arabidopsis thaliana OX=3702 GN=CYP735A2 PE=1 SV=1)

HSP 1 Score: 708.4 bits (1827), Expect = 6.1e-203
Identity = 352/523 (67.30%), Postives = 421/523 (80.50%), Query Frame = 0

Query: 1   MAVTFALVVLTTLALAAATSFFIFSLLYHTLSSYCLTPRRIKNKMAKQGVRGPKPRPLLG 60
           + + + LV++ TL         I  +LY ++  Y LTPRRIK  M +QG+ GPKPR L G
Sbjct: 5   LVLKYVLVIVMTL---------ILRVLYDSICCYFLTPRRIKKFMERQGITGPKPRLLTG 64

Query: 61  NILDVAALLSKSTSSDMPSISHDLVPRLLPHFLAWSAQFGKRFIFWNGIEPRMCLTETDL 120
           NI+D++ +LS S S+D  SI H++VPRLLPH+++WS Q+GKRFI WNG EPR+CLTET++
Sbjct: 65  NIIDISKMLSHSASNDCSSIHHNIVPRLLPHYVSWSKQYGKRFIMWNGTEPRLCLTETEM 124

Query: 121 IKELLSKYSAVSGKSWLQQQGSKHFIGRGLLMANGQNWFHQRHIVAPAFVGDRLKSYAGY 180
           IKELL+K++ V+GKSWLQQQG+K FIGRGLLMANG+ W HQRH+ APAF  DRLK YA +
Sbjct: 125 IKELLTKHNPVTGKSWLQQQGTKGFIGRGLLMANGEAWHHQRHMAAPAFTRDRLKGYAKH 184

Query: 181 MVECTREMLQSIENEVKSGRSEFEIGEYMTRLTADIISRTEFESSYEKGKQIFHLLTVLQ 240
           MVECT+ M + +  EV     E EIGE M RLTADIISRTEF SS +KGK++F LLTVLQ
Sbjct: 185 MVECTKMMAERLRKEV---GEEVEIGEEMRRLTADIISRTEFGSSCDKGKELFSLLTVLQ 244

Query: 241 HLCAQASRHLCLPGSRFFPSKYNREIKALKGKVEGLLMEIIQSRRDCVEIGRSSSYGNDL 300
            LCAQA+RHLC PGSRF PSKYNREIK+LK +VE LLMEII SR+D VEIGRSSSYG+DL
Sbjct: 245 RLCAQATRHLCFPGSRFLPSKYNREIKSLKTEVERLLMEIIDSRKDSVEIGRSSSYGDDL 304

Query: 301 LGMLLNEMQKKKDGNGLSLNLQIIMDECKTFFFAGHETTALLLTWTVMLLATNPSWQQKV 360
           LG+LLN+M    D N  +LN+Q+IMDECKTFFF GHETT+LLLTWT+MLLA NP+WQ  V
Sbjct: 305 LGLLLNQM----DSNKNNLNVQMIMDECKTFFFTGHETTSLLLTWTLMLLAHNPTWQDNV 364

Query: 361 RAEVMTVCNGQ-TPSLDHLSKLSLLNMVINESLRLYPPASILPRMAFEDIKLGDLEIPKG 420
           R EV  VC     PS++ LS L+ LN VINESLRLYPPA++LPRMAFEDIKLGDL IPKG
Sbjct: 365 RDEVRQVCGQDGVPSVEQLSSLTSLNKVINESLRLYPPATLLPRMAFEDIKLGDLIIPKG 424

Query: 421 LSIWIPVLAIHHSEELWGKDVNEFNPERFSNSKAFTSGRFIPFASGPRNCVGQSFALMET 480
           LSIWIPVLAIHHS ELWG+D NEFNPERF+     +S  F+PFA+GPRNC+GQ+FA+ME 
Sbjct: 425 LSIWIPVLAIHHSNELWGEDANEFNPERFTTRSFASSRHFMPFAAGPRNCIGQTFAMMEA 484

Query: 481 KIILAMLISKFSFTISDNYRHAPVVVLTIKPKYGVQVCLKPLN 523
           KIILAML+SKFSF IS+NYRHAP+VVLTIKPKYGVQ+ LKPL+
Sbjct: 485 KIILAMLVSKFSFAISENYRHAPIVVLTIKPKYGVQLVLKPLD 511

BLAST of Lag0030208 vs. ExPASy Swiss-Prot
Match: F4IK45 (Cytochrome P450 709B2 OS=Arabidopsis thaliana OX=3702 GN=CYP709B2 PE=2 SV=1)

HSP 1 Score: 366.3 bits (939), Expect = 5.7e-100
Identity = 200/519 (38.54%), Postives = 298/519 (57.42%), Query Frame = 0

Query: 15  LAAATSFFIFSLLYHTLSSYCLTPRRIKNKMAKQGVRGPKPRPLLGNILDVAALLSKSTS 74
           LA A    +   +Y         P  +  +  KQG+ GPK R L GN+ ++  + +++  
Sbjct: 65  LAIALVLLVVPKIYGACRILVWRPWMLSRRFKKQGISGPKYRILYGNLREIRKMKNEAKL 124

Query: 75  SDMPSISHDLVPRLLPHFLAWSAQFGKRFIFWNGIEPRMCLTETDLIKELLSKYSAVSGK 134
             +   S+D+VPR+LPH   W +Q+G+ F++W G +PR+C+++ +L K++LS       K
Sbjct: 125 MVLDPNSNDIVPRVLPHLQQWKSQYGETFLYWQGTDPRLCISDHELAKQILSNKFVFFSK 184

Query: 135 SWLQQQGSKHFIGRGLLMANGQNWFHQRHIVAPAFVGDRLKSYAGYMVECTREMLQSIEN 194
           S  + +  K   G GL+  NG +W   R I+ PAF  D+LK     MV+CT  M    + 
Sbjct: 185 SKTKPEILK-LSGNGLIFVNGLDWVRHRRILNPAFSMDKLKLMTQLMVDCTFRMFLEWKK 244

Query: 195 EVKSGRSE--FEIGEYMTRLTADIISRTEFESSYEKGKQIFHLLTVLQHLCAQASRHLCL 254
           +     +E    I     RLTADII+   F SSY +G ++F     LQ  CA A   L  
Sbjct: 245 QRNGVETEQFVLISREFKRLTADIIATAAFGSSYAEGIEVFKSQLELQKCCAAALTDLYF 304

Query: 255 PGSRFFPSKYNREIKALKGKVEGLLMEIIQSRRDCVEIGRSSSYGNDLLGMLL-----NE 314
           PG ++ P+  N +I  L  KV   +  II +R        S  YGNDLLG++L     NE
Sbjct: 305 PGIQYLPTPSNLQIWKLDMKVNSSIKRIIDARL----TSESKDYGNDLLGIMLTAASSNE 364

Query: 315 MQKKKDGNGLSLNLQIIMDECKTFFFAGHETTALLLTWTVMLLATNPSWQQKVRAEVMTV 374
            +KK       +++  I++ECKTFFFAGHETTA LLTW+ MLL+ +  WQ+K+R EV   
Sbjct: 365 SEKK-------MSIDEIIEECKTFFFAGHETTANLLTWSTMLLSLHQDWQEKLREEVFNE 424

Query: 375 C-NGQTPSLDHLSKLSLLNMVINESLRLYPPASILPRMAFEDIKLGDLEIPKGLSIWIPV 434
           C   + P  +  SKL L+N V  ESLRLY P   L R+A ED+KLG+LEIPKG +I +P+
Sbjct: 425 CGKDKIPDAETCSKLKLMNTVFMESLRLYGPVLNLLRLASEDMKLGNLEIPKGTTIILPI 484

Query: 435 LAIHHSEELWGKDVNEFNPERFSNSKAFTSGR---FIPFASGPRNCVGQSFALMETKIIL 494
             +H  + +WG D ++FNP RF+N  +  +      + F+ GPR C+GQ+FA+ME K +L
Sbjct: 485 AKMHRDKAVWGSDADKFNPMRFANGLSRAANHPNALLAFSMGPRACIGQNFAIMEAKTVL 544

Query: 495 AMLISKFSFTISDNYRHAPVVVLTIKPKYGVQVCLKPLN 523
           AM++ +F   +S +Y+HAP   LT++P+Y + V L+P++
Sbjct: 545 AMILQRFRLNLSADYKHAPADHLTLQPQYDLPVILEPID 571

BLAST of Lag0030208 vs. ExPASy Swiss-Prot
Match: Q9ASR3 (Cytochrome P450 709B1 OS=Arabidopsis thaliana OX=3702 GN=CYP709B1 PE=2 SV=1)

HSP 1 Score: 364.8 bits (935), Expect = 1.7e-99
Identity = 205/534 (38.39%), Postives = 317/534 (59.36%), Query Frame = 0

Query: 1   MAVTFALVVL-TTLALAAATSFFIFSLLYHTLSSYCLTPRRIKNKMAKQGVRGPKPRPLL 60
           + +  AL+VL   + L    +F I  L++H    + LT RR+KN    QG+ GP  R   
Sbjct: 3   LVIFLALIVLILIIGLRIFKAFMI--LVWH---PFVLT-RRLKN----QGISGPNYRIFY 62

Query: 61  GNILDVAALLSKSTSSDMPSISHDLVPRLLPHFLAWSAQFGKRFIFWNGIEPRMCLTETD 120
           GN+ ++  +  +S  S +   S+D+ PR+LPH+  W +Q+G+ F++WNG EPR+C+++ +
Sbjct: 63  GNLSEIKKMKRESHLSILDPSSNDIFPRILPHYQKWMSQYGETFLYWNGTEPRICISDPE 122

Query: 121 LIKELLSKYSAVSGKSWLQQQGSKHFIGRGLLMANGQNWFHQRHIVAPAFVGDRLKSYAG 180
           L K +LS       KS  + +  K    +GL+   G +W   R I+ PAF  DRLK    
Sbjct: 123 LAKTMLSNKLGFFVKSKARPEAVKLVGSKGLVFIEGADWVRHRRILNPAFSIDRLKIMTT 182

Query: 181 YMVECTREML-----QSIENEVKSGRSEFEIGEYMTRLTADIISRTEFESSYEKGKQIFH 240
            MV+CT +ML     +S + E +  + + E+ E   RLTADII+ + F SSY +G ++F 
Sbjct: 183 VMVDCTLKMLEEWRKESTKEETEHPKIKKEMNEEFQRLTADIIATSAFGSSYVEGIEVFR 242

Query: 241 LLTVLQHLCAQASRHLCLPGSRFFPSKYNREIKALKGKVEGLLMEIIQSRRDCVEIGRSS 300
               L+     +   + +PG+++ P+  N  +  L+ K++  +  II SR     +   S
Sbjct: 243 SQMELKRCYTTSLNQVSIPGTQYLPTPSNIRVWKLERKMDNSIKRIISSR-----LQSKS 302

Query: 301 SYGNDLLGMLLNEMQKKKDGNGLSLNLQIIMDECKTFFFAGHETTALLLTWTVMLLATNP 360
            YG+DLLG+LL       +G    ++++ I+ EC+TFFF GHETT+ LL WT MLL+ + 
Sbjct: 303 DYGDDLLGILLKAY--NTEGKERKMSIEEIIHECRTFFFGGHETTSNLLAWTTMLLSLHQ 362

Query: 361 SWQQKVRAEVMTVCNGQ-TPSLDHLSKLSLLNMVINESLRLYPPASILPRMAFEDIKLGD 420
            WQ+K+R E+   C  + TP  +  SKL L+NMVI ESLRLY P S L R A  +IKLGD
Sbjct: 363 DWQEKLREEIFKECGKEKTPDSETFSKLKLMNMVIMESLRLYGPVSALAREASVNIKLGD 422

Query: 421 LEIPKGLSIWIPVLAIHHSEELWGKDVNEFNPERFSNSKAFTSGR---FIPFASGPRNCV 480
           LEIPKG ++ IP+L +H  + LWG D ++FNP RF+N  +  +      + F+ GPR C+
Sbjct: 423 LEIPKGTTVVIPLLKMHSDKTLWGSDADKFNPMRFANGVSRAANHPNALLAFSVGPRACI 482

Query: 481 GQSFALMETKIILAMLISKFSF-TISDNYRHAPVVVLTIKPKYGVQVCLKPLNE 524
           GQ+F ++E K +L M++ +F F ++ D Y+H PV  +TI+P+YG+ V L+PL +
Sbjct: 483 GQNFVMIEAKTVLTMILQRFRFISLCDEYKHTPVDNVTIQPQYGLPVMLQPLED 519

BLAST of Lag0030208 vs. ExPASy Swiss-Prot
Match: O48786 (Cytochrome P450 734A1 OS=Arabidopsis thaliana OX=3702 GN=CYP734A1 PE=2 SV=1)

HSP 1 Score: 347.1 bits (889), Expect = 3.6e-94
Identity = 179/493 (36.31%), Postives = 288/493 (58.42%), Query Frame = 0

Query: 38  PRRIKNKMAKQGVRGPKPRPLLGNILDVAALLSKSTSSDMPSISHDLVPRLLPHFLAWSA 97
           PR+I+   +KQG+RGP     +GN+ ++  ++ K++S  MP  SH+++PR+L  +  W  
Sbjct: 35  PRKIEEHFSKQGIRGPPYHFFIGNVKELVGMMLKASSHPMP-FSHNILPRVLSFYHHWRK 94

Query: 98  QFGKRFIFWNGIEPRMCLTETDLIKELLSKYSAVSGKSWLQQQGSKHFIGRGLLMANGQN 157
            +G  F+ W G   R+ + + DLI+E+ SK               K   G GLL   G+ 
Sbjct: 95  IYGATFLVWFGPTFRLTVADPDLIREIFSKSEFYEKNE--AHPLVKQLEGDGLLSLKGEK 154

Query: 158 WFHQRHIVAPAFVGDRLKSYAGYMVECTREMLQSIENEV-KSGRSEFEIGEYMTRLTADI 217
           W H R I++P F  + LK     +++   +M+    +++ ++G  E ++ E+   LT D+
Sbjct: 155 WAHHRKIISPTFHMENLKLLVPVVLKSVTDMVDKWSDKLSENGEVEVDVYEWFQILTEDV 214

Query: 218 ISRTEFESSYEKGKQIFHLLTVLQHLCAQASRHLCLPGSRFFPSKYNREIKALKGKVEGL 277
           ISRT F SSYE G+ +F L      LCA+A + + +PG RFFP++ N +   L  ++   
Sbjct: 215 ISRTAFGSSYEDGRAVFRLQAQQMLLCAEAFQKVFIPGYRFFPTRGNLKSWKLDKEIRKS 274

Query: 278 LMEIIQSRRDCVEIGRSSS----YGNDLLGMLLNEMQKKKDGNGLSLNLQIIMDECKTFF 337
           L+++I+ RR     G           DLLG+++            ++ +Q I++ECK+FF
Sbjct: 275 LLKLIERRRQNAIDGEGEECKEPAAKDLLGLMI---------QAKNVTVQDIVEECKSFF 334

Query: 338 FAGHETTALLLTWTVMLLATNPSWQQKVRAEVMTVCNGQ-TPSLDHLSKLSLLNMVINES 397
           FAG +TT+ LLTWT +LL+ +P WQ K R EV+ VC  +  P+ DH+ KL  L+M++NES
Sbjct: 335 FAGKQTTSNLLTWTTILLSMHPEWQAKARDEVLRVCGSRDVPTKDHVVKLKTLSMILNES 394

Query: 398 LRLYPPASILPRMAFEDIKLGDLEIPKGLSIWIPVLAIHHSEELWGKDVNEFNPERFSNS 457
           LRLYPP     R A  D+KLG  +IP G  + IP++A+HH + +WG DVNEFNP RF++ 
Sbjct: 395 LRLYPPIVATIRRAKSDVKLGGYKIPCGTELLIPIIAVHHDQAIWGNDVNEFNPARFADG 454

Query: 458 KAFTSGR---FIPFASGPRNCVGQSFALMETKIILAMLISKFSFTISDNYRHAPVVVLTI 517
               +     FIPF  G R C+GQ+ A+++ K+ LA++I +F+F ++  Y+HAP V++ +
Sbjct: 455 VPRAAKHPVGFIPFGLGVRTCIGQNLAILQAKLTLAVMIQRFTFHLAPTYQHAPTVLMLL 514

Query: 518 KPKYGVQVCLKPL 522
            P++G  +  + L
Sbjct: 515 YPQHGAPITFRRL 515

BLAST of Lag0030208 vs. ExPASy TrEMBL
Match: A0A5A7T7B8 (Cytokinin hydroxylase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold64G001740 PE=3 SV=1)

HSP 1 Score: 956.8 bits (2472), Expect = 3.7e-275
Identity = 480/517 (92.84%), Postives = 494/517 (95.55%), Query Frame = 0

Query: 8   VVLTTLALAAATSFFIFSLLYHTLSSYCLTPRRIKNKMAKQGVRGPKPRPLLGNILDVAA 67
           ++LTTLAL   TSFFIFSLLY T+S Y LTPRRIK KMAKQGV GPKPRPLLGNILDVA+
Sbjct: 7   LLLTTLAL-LTTSFFIFSLLYDTVSCYLLTPRRIKQKMAKQGVHGPKPRPLLGNILDVAS 66

Query: 68  LLSKSTSSDMPSISHDLVPRLLPHFLAWSAQFGKRFIFWNGIEPRMCLTETDLIKELLSK 127
           LLSKSTSSDMPSISHDLVPRLLPHFLAWS+QFGKRFIFWNGIEPRMCLTETDLIKELLSK
Sbjct: 67  LLSKSTSSDMPSISHDLVPRLLPHFLAWSSQFGKRFIFWNGIEPRMCLTETDLIKELLSK 126

Query: 128 YSAVSGKSWLQQQGSKHFIGRGLLMANGQNWFHQRHIVAPAFVGDRLKSYAGYMVECTRE 187
           YS VSGKSWLQQQGSKHFIGRGLLMANGQNWFHQRHIVAPAFVGDRLKSYAGYMVECT+E
Sbjct: 127 YSTVSGKSWLQQQGSKHFIGRGLLMANGQNWFHQRHIVAPAFVGDRLKSYAGYMVECTKE 186

Query: 188 MLQSIENEVKSGRSEFEIGEYMTRLTADIISRTEFESSYEKGKQIFHLLTVLQHLCAQAS 247
           ML+SIE EVKSGRSEFEIGEYMTRLTADIISRTEFESS+EKGKQIFHLLTVLQHLCAQAS
Sbjct: 187 MLESIEKEVKSGRSEFEIGEYMTRLTADIISRTEFESSFEKGKQIFHLLTVLQHLCAQAS 246

Query: 248 RHLCLPGSRFFPSKYNREIKALKGKVEGLLMEIIQSRRDCVEIGRSSSYGNDLLGMLLNE 307
           RHLC PGSRFFPSKYNREIKALKGKVE LLMEIIQSR+DCVEIGRSSSYGNDLLGMLLNE
Sbjct: 247 RHLCFPGSRFFPSKYNREIKALKGKVEELLMEIIQSRKDCVEIGRSSSYGNDLLGMLLNE 306

Query: 308 MQKKK-DG-NGLSLNLQIIMDECKTFFFAGHETTALLLTWTVMLLATNPSWQQKVRAEVM 367
           MQKKK DG NGLSLNLQIIMDECKTFFFAGHETTALLLTWTVMLLATNP+WQ KVRAEVM
Sbjct: 307 MQKKKLDGNNGLSLNLQIIMDECKTFFFAGHETTALLLTWTVMLLATNPTWQDKVRAEVM 366

Query: 368 TVCNGQTPSLDHLSKLSLLNMVINESLRLYPPASILPRMAFEDIKLGDLEIPKGLSIWIP 427
            VC  +TPS  HLSKLSLL+MVINESLRLYPPASILPRMAFEDIKLGDLEIPKGLSIWIP
Sbjct: 367 AVCGSETPSFHHLSKLSLLSMVINESLRLYPPASILPRMAFEDIKLGDLEIPKGLSIWIP 426

Query: 428 VLAIHHSEELWGKDVNEFNPERFSNSKAFTSGRFIPFASGPRNCVGQSFALMETKIILAM 487
           VLAIHHSEELWGKD NEFNPERF+NSK FTSG FIPFASGPRNC+GQSFALMETKIILAM
Sbjct: 427 VLAIHHSEELWGKDANEFNPERFANSKPFTSGGFIPFASGPRNCIGQSFALMETKIILAM 486

Query: 488 LISKFSFTISDNYRHAPVVVLTIKPKYGVQVCLKPLN 523
           LISKFSFTISDNYRHAPVVVLTIKPKYGVQVCLKPLN
Sbjct: 487 LISKFSFTISDNYRHAPVVVLTIKPKYGVQVCLKPLN 522

BLAST of Lag0030208 vs. ExPASy TrEMBL
Match: A0A1S3CAS2 (cytokinin hydroxylase-like OS=Cucumis melo OX=3656 GN=LOC103498598 PE=3 SV=1)

HSP 1 Score: 955.7 bits (2469), Expect = 8.1e-275
Identity = 479/517 (92.65%), Postives = 494/517 (95.55%), Query Frame = 0

Query: 8   VVLTTLALAAATSFFIFSLLYHTLSSYCLTPRRIKNKMAKQGVRGPKPRPLLGNILDVAA 67
           ++LTTLAL   TSFFIFSLLY T+S Y LTPRRIK KMAKQGV GPKPRPLLGNILDVA+
Sbjct: 7   LLLTTLAL-LTTSFFIFSLLYDTVSCYLLTPRRIKQKMAKQGVHGPKPRPLLGNILDVAS 66

Query: 68  LLSKSTSSDMPSISHDLVPRLLPHFLAWSAQFGKRFIFWNGIEPRMCLTETDLIKELLSK 127
           LLSKSTSSDMPSISHDLVPRLLPHFLAWS+QFGKRFIFWNGIEPRMCLTETDLIKELLSK
Sbjct: 67  LLSKSTSSDMPSISHDLVPRLLPHFLAWSSQFGKRFIFWNGIEPRMCLTETDLIKELLSK 126

Query: 128 YSAVSGKSWLQQQGSKHFIGRGLLMANGQNWFHQRHIVAPAFVGDRLKSYAGYMVECTRE 187
           YS VSGKSWLQQQGSKHFIGRGLLMANGQNWFHQRHIVAPAFVGDRLKSYAGYMVECT+E
Sbjct: 127 YSTVSGKSWLQQQGSKHFIGRGLLMANGQNWFHQRHIVAPAFVGDRLKSYAGYMVECTKE 186

Query: 188 MLQSIENEVKSGRSEFEIGEYMTRLTADIISRTEFESSYEKGKQIFHLLTVLQHLCAQAS 247
           ML+SIE EVKSGRSEFEIGEYMTRLTADIISRTEFESS+EKGKQIFHLLTVLQHLCAQAS
Sbjct: 187 MLESIEKEVKSGRSEFEIGEYMTRLTADIISRTEFESSFEKGKQIFHLLTVLQHLCAQAS 246

Query: 248 RHLCLPGSRFFPSKYNREIKALKGKVEGLLMEIIQSRRDCVEIGRSSSYGNDLLGMLLNE 307
           RHLC PGSRFFPSKYNREIKALKGKVE LLMEIIQSR+DCVEIGRSSSYGNDLLGMLLNE
Sbjct: 247 RHLCFPGSRFFPSKYNREIKALKGKVEELLMEIIQSRKDCVEIGRSSSYGNDLLGMLLNE 306

Query: 308 MQKKK-DG-NGLSLNLQIIMDECKTFFFAGHETTALLLTWTVMLLATNPSWQQKVRAEVM 367
           MQKKK DG NGLSLNLQIIMDECKTFFFAGHETTALLLTWTVMLLATNP+WQ KVRAEVM
Sbjct: 307 MQKKKLDGNNGLSLNLQIIMDECKTFFFAGHETTALLLTWTVMLLATNPTWQDKVRAEVM 366

Query: 368 TVCNGQTPSLDHLSKLSLLNMVINESLRLYPPASILPRMAFEDIKLGDLEIPKGLSIWIP 427
            VC  +TPS  HLSKLSLL+MVINESLRLYPPASILPRMAFEDIKLGDLEIPKGLSIWIP
Sbjct: 367 AVCGSETPSFHHLSKLSLLSMVINESLRLYPPASILPRMAFEDIKLGDLEIPKGLSIWIP 426

Query: 428 VLAIHHSEELWGKDVNEFNPERFSNSKAFTSGRFIPFASGPRNCVGQSFALMETKIILAM 487
           +LAIHHSEELWGKD NEFNPERF+NSK FTSG FIPFASGPRNC+GQSFALMETKIILAM
Sbjct: 427 LLAIHHSEELWGKDANEFNPERFANSKPFTSGGFIPFASGPRNCIGQSFALMETKIILAM 486

Query: 488 LISKFSFTISDNYRHAPVVVLTIKPKYGVQVCLKPLN 523
           LISKFSFTISDNYRHAPVVVLTIKPKYGVQVCLKPLN
Sbjct: 487 LISKFSFTISDNYRHAPVVVLTIKPKYGVQVCLKPLN 522

BLAST of Lag0030208 vs. ExPASy TrEMBL
Match: A0A6J1FGF0 (cytokinin hydroxylase-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111445453 PE=3 SV=1)

HSP 1 Score: 950.3 bits (2455), Expect = 3.4e-273
Identity = 476/521 (91.36%), Postives = 494/521 (94.82%), Query Frame = 0

Query: 1   MAVTFALVVLTTLALAAATSFFIFSLLYHTLSSYCLTPRRIKNKMAKQGVRGPKPRPLLG 60
           MA+  AL +LTTLA  AATSF IFSLLYHTLS Y LTPRRI  KMAKQGVRGPKPRPLLG
Sbjct: 1   MAIMLAL-LLTTLAF-AATSFCIFSLLYHTLSCYWLTPRRIMRKMAKQGVRGPKPRPLLG 60

Query: 61  NILDVAALLSKSTSSDMPSISHDLVPRLLPHFLAWSAQFGKRFIFWNGIEPRMCLTETDL 120
           NILDVAALLSKSTSSDMPSISHDLVPRLLPH+LAWSAQFGKRFIFWNGIEPRMCLTET L
Sbjct: 61  NILDVAALLSKSTSSDMPSISHDLVPRLLPHYLAWSAQFGKRFIFWNGIEPRMCLTETHL 120

Query: 121 IKELLSKYSAVSGKSWLQQQGSKHFIGRGLLMANGQNWFHQRHIVAPAFVGDRLKSYAGY 180
           IKELLSKYS+VSGKSWLQQQGSKHFIGRGLLMANG NWFHQRHIVAPAFVGDRLKSYAGY
Sbjct: 121 IKELLSKYSSVSGKSWLQQQGSKHFIGRGLLMANGPNWFHQRHIVAPAFVGDRLKSYAGY 180

Query: 181 MVECTREMLQSIENEVKSGRSEFEIGEYMTRLTADIISRTEFESSYEKGKQIFHLLTVLQ 240
           MVECTREMLQS+ENEVKSGRSEFEIGEYMTRLTADIISRTEFESSYEKGKQIFHLLTVLQ
Sbjct: 181 MVECTREMLQSLENEVKSGRSEFEIGEYMTRLTADIISRTEFESSYEKGKQIFHLLTVLQ 240

Query: 241 HLCAQASRHLCLPGSRFFPSKYNREIKALKGKVEGLLMEIIQSRRDCVEIGRSSSYGNDL 300
           HLCAQASRHLCLPGSRFFPSKYNREIKALKGKVEGLLMEIIQSR+D VEIGRSSSYGNDL
Sbjct: 241 HLCAQASRHLCLPGSRFFPSKYNREIKALKGKVEGLLMEIIQSRKDGVEIGRSSSYGNDL 300

Query: 301 LGMLLNEMQKKKDGNGLSLNLQIIMDECKTFFFAGHETTALLLTWTVMLLATNPSWQQKV 360
           LGMLLNEMQKKK+GNGLSLNLQIIMDECKTFFFAGHETTALLLTWT+MLLATNPSWQ +V
Sbjct: 301 LGMLLNEMQKKKNGNGLSLNLQIIMDECKTFFFAGHETTALLLTWTIMLLATNPSWQDRV 360

Query: 361 RAEVMTVCNGQTPSLDHLSKLSLLNMVINESLRLYPPASILPRMAFEDIKLGDLEIPKGL 420
           RAE+  VCN QTPSL HLSKL LL MVINESLRLYPPASILPRMAFEDIKLGDLEIPKGL
Sbjct: 361 RAELNAVCNSQTPSLHHLSKLPLLGMVINESLRLYPPASILPRMAFEDIKLGDLEIPKGL 420

Query: 421 SIWIPVLAIHHSEELWGKDVNEFNPERFSNSKAFTSGRFIPFASGPRNCVGQSFALMETK 480
           S+WIPV+AIHHSEE+WGKD NEFNPERF +SKAFT G FIPFASGPRNCVGQ+FALMETK
Sbjct: 421 SVWIPVVAIHHSEEIWGKDANEFNPERFGSSKAFTCGGFIPFASGPRNCVGQAFALMETK 480

Query: 481 IILAMLISKFSFTISDNYRHAPVVVLTIKPKYGVQVCLKPL 522
           IILAMLISKF FTIS +YRHAPVVVLT+KPKYGVQ+CL PL
Sbjct: 481 IILAMLISKFRFTISPSYRHAPVVVLTVKPKYGVQICLTPL 519

BLAST of Lag0030208 vs. ExPASy TrEMBL
Match: A0A6J1JYD2 (cytokinin hydroxylase-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111488993 PE=3 SV=1)

HSP 1 Score: 948.0 bits (2449), Expect = 1.7e-272
Identity = 472/518 (91.12%), Postives = 490/518 (94.59%), Query Frame = 0

Query: 4   TFALVVLTTLALAAATSFFIFSLLYHTLSSYCLTPRRIKNKMAKQGVRGPKPRPLLGNIL 63
           T   ++LTTLA  A TSF IFSLLYHTLS Y LTPRRI  KMAKQGVRGPKPRPLLGNIL
Sbjct: 3   TMLALLLTTLAF-AVTSFCIFSLLYHTLSCYWLTPRRIMRKMAKQGVRGPKPRPLLGNIL 62

Query: 64  DVAALLSKSTSSDMPSISHDLVPRLLPHFLAWSAQFGKRFIFWNGIEPRMCLTETDLIKE 123
           DVAALLSKSTSSDMPSISHDLVPRLLPH+LAWSAQFGKRFIFWNGIEPRMCLTET LIKE
Sbjct: 63  DVAALLSKSTSSDMPSISHDLVPRLLPHYLAWSAQFGKRFIFWNGIEPRMCLTETHLIKE 122

Query: 124 LLSKYSAVSGKSWLQQQGSKHFIGRGLLMANGQNWFHQRHIVAPAFVGDRLKSYAGYMVE 183
           LLSKYS+VSGKSWLQQQGSKHFIGRGLLMANG NWFHQRHIVAPAFVGDRLKSYAGYMVE
Sbjct: 123 LLSKYSSVSGKSWLQQQGSKHFIGRGLLMANGPNWFHQRHIVAPAFVGDRLKSYAGYMVE 182

Query: 184 CTREMLQSIENEVKSGRSEFEIGEYMTRLTADIISRTEFESSYEKGKQIFHLLTVLQHLC 243
           CTREMLQS+ENEVKSGRSEFEIGEYMTRLTADIISRTEFESSYEKGKQIFHLLTVLQHLC
Sbjct: 183 CTREMLQSLENEVKSGRSEFEIGEYMTRLTADIISRTEFESSYEKGKQIFHLLTVLQHLC 242

Query: 244 AQASRHLCLPGSRFFPSKYNREIKALKGKVEGLLMEIIQSRRDCVEIGRSSSYGNDLLGM 303
           AQASRHLCLPGSRFFPSKYNREIKALKGKVEGLLMEIIQSR+D VEIGRSSSYGNDLLGM
Sbjct: 243 AQASRHLCLPGSRFFPSKYNREIKALKGKVEGLLMEIIQSRKDGVEIGRSSSYGNDLLGM 302

Query: 304 LLNEMQKKKDGNGLSLNLQIIMDECKTFFFAGHETTALLLTWTVMLLATNPSWQQKVRAE 363
           LLNEMQKKK+GNGLSLNLQIIMDECKTFFFAGHETTALLLTWT+MLLATNPSWQ +VRAE
Sbjct: 303 LLNEMQKKKNGNGLSLNLQIIMDECKTFFFAGHETTALLLTWTIMLLATNPSWQDRVRAE 362

Query: 364 VMTVCNGQTPSLDHLSKLSLLNMVINESLRLYPPASILPRMAFEDIKLGDLEIPKGLSIW 423
           +  VCN QTPSL HLSKL LL MVINESLRLYPPASILPRMAFEDIKLGDLEIPKGLS+W
Sbjct: 363 LNAVCNSQTPSLHHLSKLPLLGMVINESLRLYPPASILPRMAFEDIKLGDLEIPKGLSVW 422

Query: 424 IPVLAIHHSEELWGKDVNEFNPERFSNSKAFTSGRFIPFASGPRNCVGQSFALMETKIIL 483
           IPV+AIHHSEE+WGKD NEFNPERF +SKAFT G FIPFASGPRNCVGQ+FALMETKIIL
Sbjct: 423 IPVVAIHHSEEIWGKDANEFNPERFGSSKAFTCGGFIPFASGPRNCVGQAFALMETKIIL 482

Query: 484 AMLISKFSFTISDNYRHAPVVVLTIKPKYGVQVCLKPL 522
           AMLISKF FTIS +YRHAPVVVLT+KPKYGVQ+CL PL
Sbjct: 483 AMLISKFRFTISPSYRHAPVVVLTVKPKYGVQICLTPL 519

BLAST of Lag0030208 vs. ExPASy TrEMBL
Match: A0A6J1FHA5 (cytokinin hydroxylase-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111445453 PE=3 SV=1)

HSP 1 Score: 924.5 bits (2388), Expect = 2.0e-265
Identity = 468/521 (89.83%), Postives = 486/521 (93.28%), Query Frame = 0

Query: 1   MAVTFALVVLTTLALAAATSFFIFSLLYHTLSSYCLTPRRIKNKMAKQGVRGPKPRPLLG 60
           MA+  AL +LTTLA  AATSF IFSLLYHTLS Y LTPRRI  KMAKQGVRGPKPRPLLG
Sbjct: 1   MAIMLAL-LLTTLAF-AATSFCIFSLLYHTLSCYWLTPRRIMRKMAKQGVRGPKPRPLLG 60

Query: 61  NILDVAALLSKSTSSDMPSISHDLVPRLLPHFLAWSAQFGKRFIFWNGIEPRMCLTETDL 120
           NILDVAALLSKSTSSDMPSISHDLVPRLLPH+LAWSAQFGKRFIFWNGIEPRMCLTET L
Sbjct: 61  NILDVAALLSKSTSSDMPSISHDLVPRLLPHYLAWSAQFGKRFIFWNGIEPRMCLTETHL 120

Query: 121 IKELLSKYSAVSGKSWLQQQGSKHFIGRGLLMANGQNWFHQRHIVAPAFVGDRLKSYAGY 180
           IKELLSKYS+VSGKSWLQQQGSKHFIGRGLLMANG NWFHQRHIVAPAFVGDRLKSYAGY
Sbjct: 121 IKELLSKYSSVSGKSWLQQQGSKHFIGRGLLMANGPNWFHQRHIVAPAFVGDRLKSYAGY 180

Query: 181 MVECTREMLQSIENEVKSGRSEFEIGEYMTRLTADIISRTEFESSYEKGKQIFHLLTVLQ 240
           MVECTREMLQS+ENEVKSGRSEFEIGEYMTRLTADIISRTEFESSYEKGKQIFHLLTVLQ
Sbjct: 181 MVECTREMLQSLENEVKSGRSEFEIGEYMTRLTADIISRTEFESSYEKGKQIFHLLTVLQ 240

Query: 241 HLCAQASRHLCLPGSRFFPSKYNREIKALKGKVEGLLMEIIQSRRDCVEIGRSSSYGNDL 300
           HLCAQASRHLCLPGS        REIKALKGKVEGLLMEIIQSR+D VEIGRSSSYGNDL
Sbjct: 241 HLCAQASRHLCLPGS--------REIKALKGKVEGLLMEIIQSRKDGVEIGRSSSYGNDL 300

Query: 301 LGMLLNEMQKKKDGNGLSLNLQIIMDECKTFFFAGHETTALLLTWTVMLLATNPSWQQKV 360
           LGMLLNEMQKKK+GNGLSLNLQIIMDECKTFFFAGHETTALLLTWT+MLLATNPSWQ +V
Sbjct: 301 LGMLLNEMQKKKNGNGLSLNLQIIMDECKTFFFAGHETTALLLTWTIMLLATNPSWQDRV 360

Query: 361 RAEVMTVCNGQTPSLDHLSKLSLLNMVINESLRLYPPASILPRMAFEDIKLGDLEIPKGL 420
           RAE+  VCN QTPSL HLSKL LL MVINESLRLYPPASILPRMAFEDIKLGDLEIPKGL
Sbjct: 361 RAELNAVCNSQTPSLHHLSKLPLLGMVINESLRLYPPASILPRMAFEDIKLGDLEIPKGL 420

Query: 421 SIWIPVLAIHHSEELWGKDVNEFNPERFSNSKAFTSGRFIPFASGPRNCVGQSFALMETK 480
           S+WIPV+AIHHSEE+WGKD NEFNPERF +SKAFT G FIPFASGPRNCVGQ+FALMETK
Sbjct: 421 SVWIPVVAIHHSEEIWGKDANEFNPERFGSSKAFTCGGFIPFASGPRNCVGQAFALMETK 480

Query: 481 IILAMLISKFSFTISDNYRHAPVVVLTIKPKYGVQVCLKPL 522
           IILAMLISKF FTIS +YRHAPVVVLT+KPKYGVQ+CL PL
Sbjct: 481 IILAMLISKFRFTISPSYRHAPVVVLTVKPKYGVQICLTPL 511

BLAST of Lag0030208 vs. TAIR 10
Match: AT5G38450.1 (cytochrome P450, family 735, subfamily A, polypeptide 1 )

HSP 1 Score: 733.0 bits (1891), Expect = 1.7e-211
Identity = 365/518 (70.46%), Postives = 432/518 (83.40%), Query Frame = 0

Query: 7   LVVLTTLALAAATSFFIFSLLYHTLSSYCLTPRRIKNKMAKQGVRGPKPRPLLGNILDVA 66
           L +L +L +   T+  I  +LY T+S Y LTPRRIK  M +QGV GPKPRPL GNIL+++
Sbjct: 3   LTILKSLLVIFVTT--ILRVLYDTISCYWLTPRRIKKIMEQQGVTGPKPRPLTGNILEIS 62

Query: 67  ALLSKSTSSDMPSISHDLVPRLLPHFLAWSAQFGKRFIFWNGIEPRMCLTETDLIKELLS 126
           A++S+S S D  SI HD+V RLLPH++AWS Q+GKRFI WNG +PR+CLTET+LIKELL 
Sbjct: 63  AMVSQSASKDCDSIHHDIVGRLLPHYVAWSKQYGKRFIVWNGTDPRLCLTETELIKELLM 122

Query: 127 KYSAVSGKSWLQQQGSKHFIGRGLLMANGQNWFHQRHIVAPAFVGDRLKSYAGYMVECTR 186
           K++ VSG+SWLQQQG+K+FIGRGLLMANGQ+W HQRH+ APAF G+RLK YA +MVECT 
Sbjct: 123 KHNGVSGRSWLQQQGTKNFIGRGLLMANGQDWHHQRHLAAPAFTGERLKGYARHMVECTS 182

Query: 187 EMLQSIENEVKSGRSEFEIGEYMTRLTADIISRTEFESSYEKGKQIFHLLTVLQHLCAQA 246
           ++++ +  EV  G +E EIGE M +LTADIISRT+F SS+EKGK++F+ LTVLQ  CAQA
Sbjct: 183 KLVERLRKEVGEGANEVEIGEEMHKLTADIISRTKFGSSFEKGKELFNHLTVLQRRCAQA 242

Query: 247 SRHLCLPGSRFFPSKYNREIKALKGKVEGLLMEIIQSRRDCVEIGRSSSYGNDLLGMLLN 306
           +RHLC PGSRF PSKYNREIK+LK +VE LL+EIIQSRRDC E+GRSS++G+DLLG+LLN
Sbjct: 243 TRHLCFPGSRFLPSKYNREIKSLKKEVERLLIEIIQSRRDCAEMGRSSTHGDDLLGLLLN 302

Query: 307 EMQKKKDGNGLSLNLQIIMDECKTFFFAGHETTALLLTWTVMLLATNPSWQQKVRAEVMT 366
           EM   K+ N  + NLQ+IMDECKTFFFAGHETTALLLTWT MLLA NP+WQ+KVR EV  
Sbjct: 303 EMDIDKNNNNNNNNLQLIMDECKTFFFAGHETTALLLTWTTMLLADNPTWQEKVREEVRE 362

Query: 367 VC--NGQTPSLDHLSKLSLLNMVINESLRLYPPASILPRMAFEDIKLGDLEIPKGLSIWI 426
           V   NG  PS+D LSKL+ L+ VINESLRLYPPA++LPRMAFED+KLGDL IPKGLSIWI
Sbjct: 363 VFGRNG-LPSVDQLSKLTSLSKVINESLRLYPPATLLPRMAFEDLKLGDLTIPKGLSIWI 422

Query: 427 PVLAIHHSEELWGKDVNEFNPERFSNSKAFTSGR-FIPFASGPRNCVGQSFALMETKIIL 486
           PVLAIHHSEELWGKD N+FNPERF   + F SGR FIPFA+GPRNC+GQ FALME KIIL
Sbjct: 423 PVLAIHHSEELWGKDANQFNPERF-GGRPFASGRHFIPFAAGPRNCIGQQFALMEAKIIL 482

Query: 487 AMLISKFSFTISDNYRHAPVVVLTIKPKYGVQVCLKPL 522
           A LISKF+FTIS NYRHAP+VVLTIKPKYGVQV LKPL
Sbjct: 483 ATLISKFNFTISKNYRHAPIVVLTIKPKYGVQVILKPL 516

BLAST of Lag0030208 vs. TAIR 10
Match: AT1G67110.1 (cytochrome P450, family 735, subfamily A, polypeptide 2 )

HSP 1 Score: 708.4 bits (1827), Expect = 4.4e-204
Identity = 352/523 (67.30%), Postives = 421/523 (80.50%), Query Frame = 0

Query: 1   MAVTFALVVLTTLALAAATSFFIFSLLYHTLSSYCLTPRRIKNKMAKQGVRGPKPRPLLG 60
           + + + LV++ TL         I  +LY ++  Y LTPRRIK  M +QG+ GPKPR L G
Sbjct: 5   LVLKYVLVIVMTL---------ILRVLYDSICCYFLTPRRIKKFMERQGITGPKPRLLTG 64

Query: 61  NILDVAALLSKSTSSDMPSISHDLVPRLLPHFLAWSAQFGKRFIFWNGIEPRMCLTETDL 120
           NI+D++ +LS S S+D  SI H++VPRLLPH+++WS Q+GKRFI WNG EPR+CLTET++
Sbjct: 65  NIIDISKMLSHSASNDCSSIHHNIVPRLLPHYVSWSKQYGKRFIMWNGTEPRLCLTETEM 124

Query: 121 IKELLSKYSAVSGKSWLQQQGSKHFIGRGLLMANGQNWFHQRHIVAPAFVGDRLKSYAGY 180
           IKELL+K++ V+GKSWLQQQG+K FIGRGLLMANG+ W HQRH+ APAF  DRLK YA +
Sbjct: 125 IKELLTKHNPVTGKSWLQQQGTKGFIGRGLLMANGEAWHHQRHMAAPAFTRDRLKGYAKH 184

Query: 181 MVECTREMLQSIENEVKSGRSEFEIGEYMTRLTADIISRTEFESSYEKGKQIFHLLTVLQ 240
           MVECT+ M + +  EV     E EIGE M RLTADIISRTEF SS +KGK++F LLTVLQ
Sbjct: 185 MVECTKMMAERLRKEV---GEEVEIGEEMRRLTADIISRTEFGSSCDKGKELFSLLTVLQ 244

Query: 241 HLCAQASRHLCLPGSRFFPSKYNREIKALKGKVEGLLMEIIQSRRDCVEIGRSSSYGNDL 300
            LCAQA+RHLC PGSRF PSKYNREIK+LK +VE LLMEII SR+D VEIGRSSSYG+DL
Sbjct: 245 RLCAQATRHLCFPGSRFLPSKYNREIKSLKTEVERLLMEIIDSRKDSVEIGRSSSYGDDL 304

Query: 301 LGMLLNEMQKKKDGNGLSLNLQIIMDECKTFFFAGHETTALLLTWTVMLLATNPSWQQKV 360
           LG+LLN+M    D N  +LN+Q+IMDECKTFFF GHETT+LLLTWT+MLLA NP+WQ  V
Sbjct: 305 LGLLLNQM----DSNKNNLNVQMIMDECKTFFFTGHETTSLLLTWTLMLLAHNPTWQDNV 364

Query: 361 RAEVMTVCNGQ-TPSLDHLSKLSLLNMVINESLRLYPPASILPRMAFEDIKLGDLEIPKG 420
           R EV  VC     PS++ LS L+ LN VINESLRLYPPA++LPRMAFEDIKLGDL IPKG
Sbjct: 365 RDEVRQVCGQDGVPSVEQLSSLTSLNKVINESLRLYPPATLLPRMAFEDIKLGDLIIPKG 424

Query: 421 LSIWIPVLAIHHSEELWGKDVNEFNPERFSNSKAFTSGRFIPFASGPRNCVGQSFALMET 480
           LSIWIPVLAIHHS ELWG+D NEFNPERF+     +S  F+PFA+GPRNC+GQ+FA+ME 
Sbjct: 425 LSIWIPVLAIHHSNELWGEDANEFNPERFTTRSFASSRHFMPFAAGPRNCIGQTFAMMEA 484

Query: 481 KIILAMLISKFSFTISDNYRHAPVVVLTIKPKYGVQVCLKPLN 523
           KIILAML+SKFSF IS+NYRHAP+VVLTIKPKYGVQ+ LKPL+
Sbjct: 485 KIILAMLVSKFSFAISENYRHAPIVVLTIKPKYGVQLVLKPLD 511

BLAST of Lag0030208 vs. TAIR 10
Match: AT2G46950.1 (cytochrome P450, family 709, subfamily B, polypeptide 2 )

HSP 1 Score: 366.3 bits (939), Expect = 4.1e-101
Identity = 200/519 (38.54%), Postives = 298/519 (57.42%), Query Frame = 0

Query: 15  LAAATSFFIFSLLYHTLSSYCLTPRRIKNKMAKQGVRGPKPRPLLGNILDVAALLSKSTS 74
           LA A    +   +Y         P  +  +  KQG+ GPK R L GN+ ++  + +++  
Sbjct: 65  LAIALVLLVVPKIYGACRILVWRPWMLSRRFKKQGISGPKYRILYGNLREIRKMKNEAKL 124

Query: 75  SDMPSISHDLVPRLLPHFLAWSAQFGKRFIFWNGIEPRMCLTETDLIKELLSKYSAVSGK 134
             +   S+D+VPR+LPH   W +Q+G+ F++W G +PR+C+++ +L K++LS       K
Sbjct: 125 MVLDPNSNDIVPRVLPHLQQWKSQYGETFLYWQGTDPRLCISDHELAKQILSNKFVFFSK 184

Query: 135 SWLQQQGSKHFIGRGLLMANGQNWFHQRHIVAPAFVGDRLKSYAGYMVECTREMLQSIEN 194
           S  + +  K   G GL+  NG +W   R I+ PAF  D+LK     MV+CT  M    + 
Sbjct: 185 SKTKPEILK-LSGNGLIFVNGLDWVRHRRILNPAFSMDKLKLMTQLMVDCTFRMFLEWKK 244

Query: 195 EVKSGRSE--FEIGEYMTRLTADIISRTEFESSYEKGKQIFHLLTVLQHLCAQASRHLCL 254
           +     +E    I     RLTADII+   F SSY +G ++F     LQ  CA A   L  
Sbjct: 245 QRNGVETEQFVLISREFKRLTADIIATAAFGSSYAEGIEVFKSQLELQKCCAAALTDLYF 304

Query: 255 PGSRFFPSKYNREIKALKGKVEGLLMEIIQSRRDCVEIGRSSSYGNDLLGMLL-----NE 314
           PG ++ P+  N +I  L  KV   +  II +R        S  YGNDLLG++L     NE
Sbjct: 305 PGIQYLPTPSNLQIWKLDMKVNSSIKRIIDARL----TSESKDYGNDLLGIMLTAASSNE 364

Query: 315 MQKKKDGNGLSLNLQIIMDECKTFFFAGHETTALLLTWTVMLLATNPSWQQKVRAEVMTV 374
            +KK       +++  I++ECKTFFFAGHETTA LLTW+ MLL+ +  WQ+K+R EV   
Sbjct: 365 SEKK-------MSIDEIIEECKTFFFAGHETTANLLTWSTMLLSLHQDWQEKLREEVFNE 424

Query: 375 C-NGQTPSLDHLSKLSLLNMVINESLRLYPPASILPRMAFEDIKLGDLEIPKGLSIWIPV 434
           C   + P  +  SKL L+N V  ESLRLY P   L R+A ED+KLG+LEIPKG +I +P+
Sbjct: 425 CGKDKIPDAETCSKLKLMNTVFMESLRLYGPVLNLLRLASEDMKLGNLEIPKGTTIILPI 484

Query: 435 LAIHHSEELWGKDVNEFNPERFSNSKAFTSGR---FIPFASGPRNCVGQSFALMETKIIL 494
             +H  + +WG D ++FNP RF+N  +  +      + F+ GPR C+GQ+FA+ME K +L
Sbjct: 485 AKMHRDKAVWGSDADKFNPMRFANGLSRAANHPNALLAFSMGPRACIGQNFAIMEAKTVL 544

Query: 495 AMLISKFSFTISDNYRHAPVVVLTIKPKYGVQVCLKPLN 523
           AM++ +F   +S +Y+HAP   LT++P+Y + V L+P++
Sbjct: 545 AMILQRFRLNLSADYKHAPADHLTLQPQYDLPVILEPID 571

BLAST of Lag0030208 vs. TAIR 10
Match: AT2G46960.2 (cytochrome P450, family 709, subfamily B, polypeptide 1 )

HSP 1 Score: 364.8 bits (935), Expect = 1.2e-100
Identity = 205/534 (38.39%), Postives = 317/534 (59.36%), Query Frame = 0

Query: 1   MAVTFALVVL-TTLALAAATSFFIFSLLYHTLSSYCLTPRRIKNKMAKQGVRGPKPRPLL 60
           + +  AL+VL   + L    +F I  L++H    + LT RR+KN    QG+ GP  R   
Sbjct: 3   LVIFLALIVLILIIGLRIFKAFMI--LVWH---PFVLT-RRLKN----QGISGPNYRIFY 62

Query: 61  GNILDVAALLSKSTSSDMPSISHDLVPRLLPHFLAWSAQFGKRFIFWNGIEPRMCLTETD 120
           GN+ ++  +  +S  S +   S+D+ PR+LPH+  W +Q+G+ F++WNG EPR+C+++ +
Sbjct: 63  GNLSEIKKMKRESHLSILDPSSNDIFPRILPHYQKWMSQYGETFLYWNGTEPRICISDPE 122

Query: 121 LIKELLSKYSAVSGKSWLQQQGSKHFIGRGLLMANGQNWFHQRHIVAPAFVGDRLKSYAG 180
           L K +LS       KS  + +  K    +GL+   G +W   R I+ PAF  DRLK    
Sbjct: 123 LAKTMLSNKLGFFVKSKARPEAVKLVGSKGLVFIEGADWVRHRRILNPAFSIDRLKIMTT 182

Query: 181 YMVECTREML-----QSIENEVKSGRSEFEIGEYMTRLTADIISRTEFESSYEKGKQIFH 240
            MV+CT +ML     +S + E +  + + E+ E   RLTADII+ + F SSY +G ++F 
Sbjct: 183 VMVDCTLKMLEEWRKESTKEETEHPKIKKEMNEEFQRLTADIIATSAFGSSYVEGIEVFR 242

Query: 241 LLTVLQHLCAQASRHLCLPGSRFFPSKYNREIKALKGKVEGLLMEIIQSRRDCVEIGRSS 300
               L+     +   + +PG+++ P+  N  +  L+ K++  +  II SR     +   S
Sbjct: 243 SQMELKRCYTTSLNQVSIPGTQYLPTPSNIRVWKLERKMDNSIKRIISSR-----LQSKS 302

Query: 301 SYGNDLLGMLLNEMQKKKDGNGLSLNLQIIMDECKTFFFAGHETTALLLTWTVMLLATNP 360
            YG+DLLG+LL       +G    ++++ I+ EC+TFFF GHETT+ LL WT MLL+ + 
Sbjct: 303 DYGDDLLGILLKAY--NTEGKERKMSIEEIIHECRTFFFGGHETTSNLLAWTTMLLSLHQ 362

Query: 361 SWQQKVRAEVMTVCNGQ-TPSLDHLSKLSLLNMVINESLRLYPPASILPRMAFEDIKLGD 420
            WQ+K+R E+   C  + TP  +  SKL L+NMVI ESLRLY P S L R A  +IKLGD
Sbjct: 363 DWQEKLREEIFKECGKEKTPDSETFSKLKLMNMVIMESLRLYGPVSALAREASVNIKLGD 422

Query: 421 LEIPKGLSIWIPVLAIHHSEELWGKDVNEFNPERFSNSKAFTSGR---FIPFASGPRNCV 480
           LEIPKG ++ IP+L +H  + LWG D ++FNP RF+N  +  +      + F+ GPR C+
Sbjct: 423 LEIPKGTTVVIPLLKMHSDKTLWGSDADKFNPMRFANGVSRAANHPNALLAFSVGPRACI 482

Query: 481 GQSFALMETKIILAMLISKFSF-TISDNYRHAPVVVLTIKPKYGVQVCLKPLNE 524
           GQ+F ++E K +L M++ +F F ++ D Y+H PV  +TI+P+YG+ V L+PL +
Sbjct: 483 GQNFVMIEAKTVLTMILQRFRFISLCDEYKHTPVDNVTIQPQYGLPVMLQPLED 519

BLAST of Lag0030208 vs. TAIR 10
Match: AT2G26710.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 347.1 bits (889), Expect = 2.6e-95
Identity = 179/493 (36.31%), Postives = 288/493 (58.42%), Query Frame = 0

Query: 38  PRRIKNKMAKQGVRGPKPRPLLGNILDVAALLSKSTSSDMPSISHDLVPRLLPHFLAWSA 97
           PR+I+   +KQG+RGP     +GN+ ++  ++ K++S  MP  SH+++PR+L  +  W  
Sbjct: 35  PRKIEEHFSKQGIRGPPYHFFIGNVKELVGMMLKASSHPMP-FSHNILPRVLSFYHHWRK 94

Query: 98  QFGKRFIFWNGIEPRMCLTETDLIKELLSKYSAVSGKSWLQQQGSKHFIGRGLLMANGQN 157
            +G  F+ W G   R+ + + DLI+E+ SK               K   G GLL   G+ 
Sbjct: 95  IYGATFLVWFGPTFRLTVADPDLIREIFSKSEFYEKNE--AHPLVKQLEGDGLLSLKGEK 154

Query: 158 WFHQRHIVAPAFVGDRLKSYAGYMVECTREMLQSIENEV-KSGRSEFEIGEYMTRLTADI 217
           W H R I++P F  + LK     +++   +M+    +++ ++G  E ++ E+   LT D+
Sbjct: 155 WAHHRKIISPTFHMENLKLLVPVVLKSVTDMVDKWSDKLSENGEVEVDVYEWFQILTEDV 214

Query: 218 ISRTEFESSYEKGKQIFHLLTVLQHLCAQASRHLCLPGSRFFPSKYNREIKALKGKVEGL 277
           ISRT F SSYE G+ +F L      LCA+A + + +PG RFFP++ N +   L  ++   
Sbjct: 215 ISRTAFGSSYEDGRAVFRLQAQQMLLCAEAFQKVFIPGYRFFPTRGNLKSWKLDKEIRKS 274

Query: 278 LMEIIQSRRDCVEIGRSSS----YGNDLLGMLLNEMQKKKDGNGLSLNLQIIMDECKTFF 337
           L+++I+ RR     G           DLLG+++            ++ +Q I++ECK+FF
Sbjct: 275 LLKLIERRRQNAIDGEGEECKEPAAKDLLGLMI---------QAKNVTVQDIVEECKSFF 334

Query: 338 FAGHETTALLLTWTVMLLATNPSWQQKVRAEVMTVCNGQ-TPSLDHLSKLSLLNMVINES 397
           FAG +TT+ LLTWT +LL+ +P WQ K R EV+ VC  +  P+ DH+ KL  L+M++NES
Sbjct: 335 FAGKQTTSNLLTWTTILLSMHPEWQAKARDEVLRVCGSRDVPTKDHVVKLKTLSMILNES 394

Query: 398 LRLYPPASILPRMAFEDIKLGDLEIPKGLSIWIPVLAIHHSEELWGKDVNEFNPERFSNS 457
           LRLYPP     R A  D+KLG  +IP G  + IP++A+HH + +WG DVNEFNP RF++ 
Sbjct: 395 LRLYPPIVATIRRAKSDVKLGGYKIPCGTELLIPIIAVHHDQAIWGNDVNEFNPARFADG 454

Query: 458 KAFTSGR---FIPFASGPRNCVGQSFALMETKIILAMLISKFSFTISDNYRHAPVVVLTI 517
               +     FIPF  G R C+GQ+ A+++ K+ LA++I +F+F ++  Y+HAP V++ +
Sbjct: 455 VPRAAKHPVGFIPFGLGVRTCIGQNLAILQAKLTLAVMIQRFTFHLAPTYQHAPTVLMLL 514

Query: 518 KPKYGVQVCLKPL 522
            P++G  +  + L
Sbjct: 515 YPQHGAPITFRRL 515