Lag0030081 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0030081 vs. NCBI nr
Match: XP_022993585.1 (kininogen-1a-like [Cucurbita maxima])

HSP 1 Score: 242.7 bits (618), Expect = 5.6e-60
Identity = 176/398 (44.22%), Postives = 217/398 (54.52%), Query Frame = 0

Query: 2   LVDVADYPGTGANVPGGHPLQKPPSPIKERKMLVDVADYPGTGANVPGGHPPQKPPSPIK 61
           ++DV DY  TG N+PGG          K RKM +DV DY  TG N+PGG       +  K
Sbjct: 134 MIDVTDYRRTGPNIPGGPHYSNDE---KSRKM-IDVTDYRRTGPNIPGG---PHYSNDEK 193

Query: 62  ERKMLVDVADYPGTGANVPGGHPPQKPPSPIKERKMLVDVADYPGTGANVPGGHPPHKPP 121
            RKM +DV DY  TG N+PGG       +  K RKM +DV DY  TG N+PGG  PH   
Sbjct: 194 SRKM-IDVTDYRRTGPNIPGG---PHYSNDEKSRKM-IDVTDYRRTGPNIPGG--PHY-S 253

Query: 122 SPIKERKMLVDVADYPGTGANVPGGHPPQKPLSPIKERKMLVDVADYPGTGANVPGGHPP 181
           +  K RKM +DV DY  TG N+PGG       S  ++ + ++DV DY  TG N+PGG   
Sbjct: 254 NDEKSRKM-IDVTDYRRTGPNIPGG----PHYSNDEKSRKMIDVTDYRRTGPNIPGG--- 313

Query: 182 QKPPSTIKERKMLVDVADYPGTGANVPGGHPPQKPPSTIKERKMLVDVADYPGTGANVPG 241
               +  K RKM +DV DY  TG N+PGG       +  K RKM +DV DY  TG N+PG
Sbjct: 314 PHYSNDEKSRKM-IDVTDYRRTGPNIPGG---PHYSNDEKSRKM-IDVTDYRRTGPNIPG 373

Query: 242 GHPPQKPPSTRKMLVDVADYPSTGANVPGGHPPQKPPSPIKERKMLVDVADYPGTGANVP 301
           G        +RKM +DV DY  TG N+PGG       +  K RKM +DV DY  TG N+P
Sbjct: 374 GPHYSNDEKSRKM-IDVTDYRRTGPNIPGG---PHYSNDEKSRKM-IDVTDYRRTGPNIP 433

Query: 302 GGHPPQKPPSPIKERKMLVDVADYPGTGANVPGGHPPQKPPSPIKERKMLVDVADYPGTG 361
           GG       +  K RKM +DV DY  TG N+PGG       +  K RKM +DV DY  TG
Sbjct: 434 GG---PHYSNDEKSRKM-IDVTDYRRTGPNIPGG---PHYSNDEKSRKM-IDVTDYRRTG 486

Query: 362 ANVPGGHPPQKPPSPIKVRKMLVDVADYPGTGANVPGG 400
            N+PGG       +  K RKM +DV DY  TG N+PGG
Sbjct: 494 PNIPGG---PHYSNDEKSRKM-IDVTDYRRTGPNIPGG 486

BLAST of Lag0030081 vs. NCBI nr
Match: XP_023549861.1 (titin-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 240.0 bits (611), Expect = 3.6e-59
Identity = 193/398 (48.49%), Postives = 218/398 (54.77%), Query Frame = 0

Query: 2    LVDVADYPGTGANVPGGHPLQKPPSPIKERKMLVDVADYPGTGANVPGGHPPQKPPSPIK 61
            +VDV DY   GAN+PGG          K RKM VDV DY   GAN+PGG       +  K
Sbjct: 666  MVDVTDYRPPGANIPGGPHFANDE---KSRKM-VDVTDYRPPGANIPGG---PHFANDEK 725

Query: 62   ERKMLVDVADYPGTGANVPGGHPPQKPPSPIKERKMLVDVADYPGTGANVPGGHPPHKPP 121
             RKM VDV DY   GAN+PGG       +  K RKM VDV DY   GAN+PGG  PH   
Sbjct: 726  SRKM-VDVTDYRPPGANIPGG---PHFANDEKSRKM-VDVTDYRPPGANIPGG--PHF-A 785

Query: 122  SPIKERKMLVDVADYPGTGANVPGGHPPQKPLSPIKERKMLVDVADYPGTGANVPGGHPP 181
            +  K RKM VDV DY   GAN+PGG       +  K RKM VDV DY   GAN+PGG   
Sbjct: 786  NDEKSRKM-VDVTDYRPPGANIPGG---PHFANDEKSRKM-VDVTDYRPPGANIPGG--- 845

Query: 182  QKPPSTIKERKMLVDVADYPGTGANVPGGHPPQKPPSTIKERKMLVDVADYPGTGANVPG 241
                +  K RKM VDV DY   GAN+PGG       +  K RKM VDV DY   GAN+PG
Sbjct: 846  PHFANDEKSRKM-VDVTDYRPPGANIPGG---PHFSNDEKSRKM-VDVTDYRPPGANIPG 905

Query: 242  GHPPQKPPSTRKMLVDVADYPSTGANVPGGHPPQKPPSPIKERKMLVDVADYPGTGANVP 301
            G        +RKM VDV DY   GAN+PGG       +  K RKM VDV DY   GAN+P
Sbjct: 906  GPHFANDEKSRKM-VDVTDYRPPGANIPGG---PHFANDEKSRKM-VDVTDYRPPGANIP 965

Query: 302  GGHPPQKPPSPIKERKMLVDVADYPGTGANVPGGHPPQKPPSPIKERKMLVDVADYPGTG 361
            GG       +  K RKM VDV DY   GAN+PGG       +  K RKM VDV DY   G
Sbjct: 966  GG---PHFANDEKSRKM-VDVTDYRPPGANIPGG---PHFANDEKSRKM-VDVTDYRPPG 1018

Query: 362  ANVPGGHPPQKPPSPIKVRKMLVDVADYPGTGANVPGG 400
            AN+PGG       +  K RKM VDVADY   GAN+PGG
Sbjct: 1026 ANIPGG---PHFSNDEKSRKM-VDVADYRPPGANIPGG 1018

BLAST of Lag0030081 vs. NCBI nr
Match: XP_022939129.1 (sporozoite surface protein 2-like [Cucurbita moschata])

HSP 1 Score: 224.9 bits (572), Expect = 1.2e-54
Identity = 183/398 (45.98%), Postives = 211/398 (53.02%), Query Frame = 0

Query: 2   LVDVADYPGTGANVPGGHPLQKPPSPIKERKMLVDVADYPGTGANVPGGHPPQKPPSPIK 61
           +VDV DY  TG N+PGG          K RKM VDV DY  TG N+PGG       +  K
Sbjct: 134 MVDVNDYRPTGPNIPGGPHYSNDE---KSRKM-VDVNDYRPTGPNIPGG---PHYSNDEK 193

Query: 62  ERKMLVDVADYPGTGANVPGGHPPQKPPSPIKERKMLVDVADYPGTGANVPGGHPPHKPP 121
            RKM VDV DY  TG N+P G       +  K RKM VDV DY  TG ++P G       
Sbjct: 194 SRKM-VDVNDYRPTGPNIPEG---PHYSNDEKSRKM-VDVNDYLPTGPSIPEGLHYSNVN 253

Query: 122 SPIKERKMLVDVADYPGTGANVPGGHPPQKPLSPIKERKMLVDVADYPGTGANVPGGHPP 181
              K RKM VDV DY  TG N+PGG       S  ++ + +VDV DY  TG N+ GG   
Sbjct: 254 HDEKSRKM-VDVTDYRRTGPNIPGG----PHYSNDEKSRKMVDVNDYRPTGPNILGG--- 313

Query: 182 QKPPSTIKERKMLVDVADYPGTGANVPGGHPPQKPPSTIKERKMLVDVADYPGTGANVPG 241
               +  K RKM VDV DY  TG N+PGG       +  K RKM VDV DY  TG N+PG
Sbjct: 314 PHYSNDEKSRKM-VDVNDYRPTGPNIPGG---PHYSNDEKSRKM-VDVNDYRPTGPNIPG 373

Query: 242 GHPPQKPPSTRKMLVDVADYPSTGANVPGGHPPQKPPSPIKERKMLVDVADYPGTGANVP 301
           G        +RKM VDV DY  TG N+PGG       +  K RKM VDV DY  TG N+P
Sbjct: 374 GPHYSNDEKSRKM-VDVNDYRPTGPNIPGG---PHYSNDEKSRKM-VDVNDYRPTGPNIP 433

Query: 302 GGHPPQKPPSPIKERKMLVDVADYPGTGANVPGGHPPQKPPSPIKERKMLVDVADYPGTG 361
           GG       +  K RKM VDV DY   GAN+PGG       +  K RKM VDV DY   G
Sbjct: 434 GG---PHYSNDEKSRKM-VDVTDYRPPGANIPGG---PHFSNDEKSRKM-VDVTDYRPPG 489

Query: 362 ANVPGGHPPQKPPSPIKVRKMLVDVADYPGTGANVPGG 400
           AN+PGG       +  K RKM VDV DY   GAN+PGG
Sbjct: 494 ANIPGG---PHFSNDEKSRKM-VDVTDYRPPGANIPGG 489

BLAST of Lag0030081 vs. NCBI nr
Match: XP_028671218.1 (collagen alpha-1(III) chain-like isoform X1 [Erpetoichthys calabaricus] >XP_028671219.1 collagen alpha-1(III) chain-like isoform X2 [Erpetoichthys calabaricus])

HSP 1 Score: 160.6 bits (405), Expect = 2.8e-35
Identity = 131/375 (34.93%), Postives = 156/375 (41.60%), Query Frame = 0

Query: 8   YPGTGANVPGGHPLQKPPSPIKERKMLVDVADYPGTGANVPGGHPPQKPPSPIKERKMLV 67
           +P    +VPGGHP   PP P            +P    +VPGGHPP KP  P        
Sbjct: 577 HPSGHPSVPGGHP---PPEP-----------GHPSGHPSVPGGHPPPKPGHP-------- 636

Query: 68  DVADYPGTGANVPGGHPPQKPPSPIKERKMLVDVADYPGTGANVPGGHPPHKPPSPIKER 127
                PG+  +VPGGHPP KP  P            +P    +VPGGHPP KP  P    
Sbjct: 637 ---SVPGSHPSVPGGHPPPKPGHPSVPGGHPPPKPGHP----SVPGGHPPPKPGHPSVPG 696

Query: 128 KMLVDVADYPGTGANVPGGHPPQKPLSPIKERKMLVDVADYP---GTGANVPGGHPPQK- 187
                   +P    +VPGGHPP KP  P  +         +P       +VPGGHPP + 
Sbjct: 697 GHPPPKPGHP----SVPGGHPPPKPGHPPPKPGHPSVPGGHPPHKPGHPSVPGGHPPPEP 756

Query: 188 --PPSTIKERKMLVDVADYPGTGANVPGGHPPQKPPSTIKERKMLVDVADYPGTGANVPG 247
             PP       +       PG   +VPGGHPP KP               +P    +VPG
Sbjct: 757 GHPPPKPGHPSVPGGHPSVPGGHPSVPGGHPPPKPGHPSVPGGHPPPKPGHP----SVPG 816

Query: 248 GHPPQKPPSTRKMLVDVADYPSTGANVPGGHPPQKPPSPIKERKMLVDVADYPGTGANVP 307
           GHPP KP            +PS    VPGGHPP KP  P            +P    +VP
Sbjct: 817 GHPPPKP-----------GHPS----VPGGHPPHKPGHPSVPGGHPPHKPGHP----SVP 876

Query: 308 GGHPPQKPPSPIKERKMLVDVADYPGTGANVPGGHPPQKPPSPIKERKMLVDVADYPGTG 367
           GGHPP KP  P            +P    +VPGGHPP KP  P            +P   
Sbjct: 877 GGHPPHKPGHPSVPGGHPPHKPGHP----SVPGGHPPHKPGHPSVPGGHPPHKPGHP--- 887

Query: 368 ANVPGGHPPQKPPSP 377
            +VPGGHPP KP  P
Sbjct: 937 -SVPGGHPPHKPGHP 887

BLAST of Lag0030081 vs. NCBI nr
Match: XP_020219887.1 (DNA-directed RNA polymerase II subunit RPB1-like [Cajanus cajan])

HSP 1 Score: 127.5 bits (319), Expect = 2.6e-25
Identity = 92/341 (26.98%), Postives = 156/341 (45.75%), Query Frame = 0

Query: 2   LVDVADYPGTGANVPGGHPLQKPPSPIKERKMLVDVADYPGTGANVPGGHPPQKPPSPIK 61
           +V++ DYP TG +    HP+        E +M++++ DYP TG        P    +   
Sbjct: 69  MVNIEDYPPTGPS----HPIASK----LEGRMMMNIEDYPPTG--------PSHLVTSKS 128

Query: 62  ERKMLVDVADYPGTGANVPGGHPPQKPPSPIKERKMLVDVADYPGTGANVP--------- 121
           E +M++++ DYP TG        P  P +   E + ++++ DYP TG + P         
Sbjct: 129 EGRMMINIEDYPPTG--------PSHPIASKSEGRTMMNIEDYPPTGPSHPVASKSEGRT 188

Query: 122 ----GGHPPHKPPSPI---KERKMLVDVADYPGTGANVPGGHPPQKPLSPIKERKMLVDV 181
                 +PP  P  PI    E + ++++ DYP TG        P  P++   E +M++++
Sbjct: 189 MMNIEDYPPTGPSHPIASKSEGRTMMNIEDYPPTG--------PSHPVTSKSEGRMMMNI 248

Query: 182 ADYPGTGANVPGGHPPQKPPSTIKERKMLVDVADYPGTGANVPGGHPPQKPPSTIKERKM 241
            DYP TG        P  P ++  + + ++++ DYP TG        P  P ++  E + 
Sbjct: 249 EDYPPTG--------PSYPIASKSKGRTMMNIEDYPPTG--------PSHPIASKSEGRT 308

Query: 242 LVDVADYPGTGANVPGGHPPQKPPSTRKMLVDVADYPSTGANVPGGHPPQKPPSPIKERK 301
           ++++  YP TG N      P    S  +M++++ DYP TG        P  P S   E +
Sbjct: 309 MINIEYYPPTGPN-----HPITSKSEGRMMMNIEDYPPTG--------PSHPISSKLEGR 340

Query: 302 MLVDVADYPGTGANVPGGHPPQKPPSPIKERKMLVDVADYP 327
           M++++ DYP TG        P  P +   E K ++   DYP
Sbjct: 369 MVMNIEDYPPTG--------PSHPATSKSEGKRMMGFKDYP 340

BLAST of Lag0030081 vs. ExPASy Swiss-Prot
Match: Q9ZQI0 (Proline-rich extensin-like protein EPR1 OS=Arabidopsis thaliana OX=3702 GN=EPR1 PE=2 SV=1)

HSP 1 Score: 96.7 bits (239), Expect = 6.5e-19
Identity = 113/399 (28.32%), Postives = 143/399 (35.84%), Query Frame = 0

Query: 20  PLQKPPSPIKERKMLVDVADYPGTGANVPGGHPP--QKPPSPIKERKMLVDVADYPGTGA 79
           P+QKPP+P     +       P T    P  +PP  QKPP+P     +       P T +
Sbjct: 86  PIQKPPTPTYSPPIYPPPIQKPPTPTYSPPIYPPPIQKPPTPTYSPPIYPPPIQKPPTPS 145

Query: 80  NVPGGHPP--QKPPSPIKERKMLVDVADYPGTGANVPGGHPP-HKPPSPIKERKMLVDVA 139
             P   PP  Q PP+P     +       P T    P   PP HKPP+PI    +     
Sbjct: 146 YSPPVKPPPVQMPPTPTYSPPIKPPPVHKPPTPTYSPPIKPPVHKPPTPIYSPPIKPPPV 205

Query: 140 DYPGTGANVPGGHPP--QKPLSPIKERKMLVDVADYPGTGANVPGGHPP--QKPPSTIKE 199
             P T    P   PP   KP +P     +       P T    P   PP   KPP+ I  
Sbjct: 206 HKPPTPIYSPPIKPPPVHKPPTPTYSPPVKPPPVHKPPTPIYSPPIKPPPVHKPPTPIYS 265

Query: 200 RKMLVDVADYPGTGANVPGGHPP--QKPPSTIKERKMLVDVADYPGTGANVPGGHPPQKP 259
             +       P T    P   PP   KPP+      +       P T    P   PP KP
Sbjct: 266 PPVKPPPVQTPPTPIYSPPVKPPPVHKPPTPTYSPPVKSPPVQKPPT----PTYSPPIKP 325

Query: 260 PSTRKMLVDVADYPSTGANVPGGHPPQKPPSPIKERKMLVDVADYPGTGANVPGGHPP-- 319
           P  +K        P+   + P   PP KPP+PI    +       P T    P   PP  
Sbjct: 326 PPVQKP-------PTPTYSPPIKPPPVKPPTPIYSPPVKPPPVHKPPTPIYSPPVKPPPV 385

Query: 320 QKPPSPIKERKMLVDVADYPGTGANVPGGHPP--QKPPSPIKERKMLVDVADYPGTGANV 379
            KPP+PI    +       P T    P   PP  QKPP+P     + +     P    + 
Sbjct: 386 HKPPTPIYSPPVKPPPIQKPPTPTYSPPIKPPPLQKPPTPTYSPPIKLPPVKPPTPIYSP 445

Query: 380 PGGHPP-QKPPSPIKVRKMLVDVADYPGTGANVPGGHPP 403
           P   PP  KPP+PI    +       P T    P   PP
Sbjct: 446 PVKPPPVHKPPTPIYSPPVKPPPVHKPPTPTYSPPIKPP 473

BLAST of Lag0030081 vs. ExPASy TrEMBL
Match: A0A6J1JYX7 (kininogen-1a-like OS=Cucurbita maxima OX=3661 GN=LOC111489536 PE=4 SV=1)

HSP 1 Score: 242.7 bits (618), Expect = 2.7e-60
Identity = 176/398 (44.22%), Postives = 217/398 (54.52%), Query Frame = 0

Query: 2   LVDVADYPGTGANVPGGHPLQKPPSPIKERKMLVDVADYPGTGANVPGGHPPQKPPSPIK 61
           ++DV DY  TG N+PGG          K RKM +DV DY  TG N+PGG       +  K
Sbjct: 134 MIDVTDYRRTGPNIPGGPHYSNDE---KSRKM-IDVTDYRRTGPNIPGG---PHYSNDEK 193

Query: 62  ERKMLVDVADYPGTGANVPGGHPPQKPPSPIKERKMLVDVADYPGTGANVPGGHPPHKPP 121
            RKM +DV DY  TG N+PGG       +  K RKM +DV DY  TG N+PGG  PH   
Sbjct: 194 SRKM-IDVTDYRRTGPNIPGG---PHYSNDEKSRKM-IDVTDYRRTGPNIPGG--PHY-S 253

Query: 122 SPIKERKMLVDVADYPGTGANVPGGHPPQKPLSPIKERKMLVDVADYPGTGANVPGGHPP 181
           +  K RKM +DV DY  TG N+PGG       S  ++ + ++DV DY  TG N+PGG   
Sbjct: 254 NDEKSRKM-IDVTDYRRTGPNIPGG----PHYSNDEKSRKMIDVTDYRRTGPNIPGG--- 313

Query: 182 QKPPSTIKERKMLVDVADYPGTGANVPGGHPPQKPPSTIKERKMLVDVADYPGTGANVPG 241
               +  K RKM +DV DY  TG N+PGG       +  K RKM +DV DY  TG N+PG
Sbjct: 314 PHYSNDEKSRKM-IDVTDYRRTGPNIPGG---PHYSNDEKSRKM-IDVTDYRRTGPNIPG 373

Query: 242 GHPPQKPPSTRKMLVDVADYPSTGANVPGGHPPQKPPSPIKERKMLVDVADYPGTGANVP 301
           G        +RKM +DV DY  TG N+PGG       +  K RKM +DV DY  TG N+P
Sbjct: 374 GPHYSNDEKSRKM-IDVTDYRRTGPNIPGG---PHYSNDEKSRKM-IDVTDYRRTGPNIP 433

Query: 302 GGHPPQKPPSPIKERKMLVDVADYPGTGANVPGGHPPQKPPSPIKERKMLVDVADYPGTG 361
           GG       +  K RKM +DV DY  TG N+PGG       +  K RKM +DV DY  TG
Sbjct: 434 GG---PHYSNDEKSRKM-IDVTDYRRTGPNIPGG---PHYSNDEKSRKM-IDVTDYRRTG 486

Query: 362 ANVPGGHPPQKPPSPIKVRKMLVDVADYPGTGANVPGG 400
            N+PGG       +  K RKM +DV DY  TG N+PGG
Sbjct: 494 PNIPGG---PHYSNDEKSRKM-IDVTDYRRTGPNIPGG 486

BLAST of Lag0030081 vs. ExPASy TrEMBL
Match: A0A6J1FG76 (sporozoite surface protein 2-like OS=Cucurbita moschata OX=3662 GN=LOC111445128 PE=4 SV=1)

HSP 1 Score: 224.9 bits (572), Expect = 5.8e-55
Identity = 183/398 (45.98%), Postives = 211/398 (53.02%), Query Frame = 0

Query: 2   LVDVADYPGTGANVPGGHPLQKPPSPIKERKMLVDVADYPGTGANVPGGHPPQKPPSPIK 61
           +VDV DY  TG N+PGG          K RKM VDV DY  TG N+PGG       +  K
Sbjct: 134 MVDVNDYRPTGPNIPGGPHYSNDE---KSRKM-VDVNDYRPTGPNIPGG---PHYSNDEK 193

Query: 62  ERKMLVDVADYPGTGANVPGGHPPQKPPSPIKERKMLVDVADYPGTGANVPGGHPPHKPP 121
            RKM VDV DY  TG N+P G       +  K RKM VDV DY  TG ++P G       
Sbjct: 194 SRKM-VDVNDYRPTGPNIPEG---PHYSNDEKSRKM-VDVNDYLPTGPSIPEGLHYSNVN 253

Query: 122 SPIKERKMLVDVADYPGTGANVPGGHPPQKPLSPIKERKMLVDVADYPGTGANVPGGHPP 181
              K RKM VDV DY  TG N+PGG       S  ++ + +VDV DY  TG N+ GG   
Sbjct: 254 HDEKSRKM-VDVTDYRRTGPNIPGG----PHYSNDEKSRKMVDVNDYRPTGPNILGG--- 313

Query: 182 QKPPSTIKERKMLVDVADYPGTGANVPGGHPPQKPPSTIKERKMLVDVADYPGTGANVPG 241
               +  K RKM VDV DY  TG N+PGG       +  K RKM VDV DY  TG N+PG
Sbjct: 314 PHYSNDEKSRKM-VDVNDYRPTGPNIPGG---PHYSNDEKSRKM-VDVNDYRPTGPNIPG 373

Query: 242 GHPPQKPPSTRKMLVDVADYPSTGANVPGGHPPQKPPSPIKERKMLVDVADYPGTGANVP 301
           G        +RKM VDV DY  TG N+PGG       +  K RKM VDV DY  TG N+P
Sbjct: 374 GPHYSNDEKSRKM-VDVNDYRPTGPNIPGG---PHYSNDEKSRKM-VDVNDYRPTGPNIP 433

Query: 302 GGHPPQKPPSPIKERKMLVDVADYPGTGANVPGGHPPQKPPSPIKERKMLVDVADYPGTG 361
           GG       +  K RKM VDV DY   GAN+PGG       +  K RKM VDV DY   G
Sbjct: 434 GG---PHYSNDEKSRKM-VDVTDYRPPGANIPGG---PHFSNDEKSRKM-VDVTDYRPPG 489

Query: 362 ANVPGGHPPQKPPSPIKVRKMLVDVADYPGTGANVPGG 400
           AN+PGG       +  K RKM VDV DY   GAN+PGG
Sbjct: 494 ANIPGG---PHFSNDEKSRKM-VDVTDYRPPGANIPGG 489

BLAST of Lag0030081 vs. ExPASy TrEMBL
Match: A0A4D6MDP1 (Uncharacterized protein OS=Vigna unguiculata OX=3917 GN=DEO72_LG6g3283 PE=4 SV=1)

HSP 1 Score: 125.2 bits (313), Expect = 6.3e-25
Identity = 93/229 (40.61%), Postives = 120/229 (52.40%), Query Frame = 0

Query: 36  DVADYPGTGANVPGGHPPQKPPSPIKERKMLVDVADYPGTGANVPGGHPPQKPPSPIKER 95
           +V DYPGTG+N     P  KPP P +      +V DYPGT +N     P  KPP P ++ 
Sbjct: 36  EVDDYPGTGSN-----PKHKPPPP-RNLNNHYEVDDYPGTRSN-----PKHKPPPP-RDL 95

Query: 96  KMLVDVADYPGTGANVPGGHPPHKPPSPIKERKMLVDVADYPGTGANVPGGHPPQKPLSP 155
               ++ DYPGTG+N     P HKPP P ++     +V DYPGTGAN     PP + L+ 
Sbjct: 96  NDHYEIDDYPGTGSN-----PKHKPPPP-RDLNNHYEVDDYPGTGANPKHKPPPSRDLND 155

Query: 156 IKERKMLVDVADYPGTGANVPGGHPPQKPPSTIKERKMLVDVADYPGTGANVPGGHPPQK 215
             E      V DYPGTG+N     P  KPP   ++     +V DY GTG+N P   PP  
Sbjct: 156 HFE------VDDYPGTGSN-----PKHKPPPP-RDLNNHYEVDDYAGTGSN-PKHKPP-- 215

Query: 216 PPSTIKERKMLVDVADYPGTGANVPGGHPPQKPPSTRKMLVDVADYPST 265
           PP  + +     +V DYPGTG+N P   PP  PPS  KM ++  D+  T
Sbjct: 216 PPRDLNDH---YEVDDYPGTGSN-PKHKPP--PPSFGKMQINEDDFEVT 225

BLAST of Lag0030081 vs. ExPASy TrEMBL
Match: A0A0K9RPM4 (Hydrophob_seed domain-containing protein OS=Spinacia oleracea OX=3562 GN=SOVF_048520 PE=4 SV=1)

HSP 1 Score: 104.0 bits (258), Expect = 1.5e-18
Identity = 90/368 (24.46%), Postives = 142/368 (38.59%), Query Frame = 0

Query: 16  PGGHPLQKPPSPIKERKMLVDVADYPGTGANVPGGHPPQKPPSPIKERKMLVDVADYPGT 75
           P G P  +PP+P+    M       P      P   PP  PP+P+    +     + P  
Sbjct: 378 PMGSPPVRPPTPMSSPPM-----TSPPIRPPTPVSSPPVNPPTPMSSPPV-----NPPTP 437

Query: 76  GANVPGGHPPQKPPSPIKERKMLVDVADYPGTGANVPGGHPPHKPPSPIKERKMLVDVAD 135
            ++ P G PP +PP+P+    M       P   ++ P   PP  P +P+     L     
Sbjct: 438 VSSPPMGSPPVRPPTPMSSPPMTSPPIRPPTPESSPPMVSPPITPSTPMPSPPTLSPPVR 497

Query: 136 YPGTGANVPGGHPPQKPLSPIKERKMLVDVADYPGTGANVPGGHPPQKPPSTIKERKMLV 195
            P   ++ P   PP +P  P+    M       P    + P   PP +PP++     M  
Sbjct: 498 PPTPVSSPPISSPPVRPPMPMSSPPMASPPIRPPTPVPSPPMASPPVRPPTS---PPMAS 557

Query: 196 DVADYPGTGANVPGGHPPQKPPSTIKERKMLVDVADYPGTGANVPGGHPPQKPP------ 255
                P   ++ P   PP +PP+ +   +M+      P    + P   PP +PP      
Sbjct: 558 PPVRPPTPVSSPPMTSPPIRPPTPVSPPQMVSPPVRPPTPLPSPPTSSPPVRPPTPPTPV 617

Query: 256 STRKMLVDVADYPSTGANVPGGHPPQKPPSPIKERKMLVDVADYPGTGANVPGGHPPQKP 315
           S+  +    A  P+  ++ P   PP +PP+P+    M       P      P   PP +P
Sbjct: 618 SSPPIASPPARPPTPVSSPPVASPPIRPPTPVSSPPM-----SSPPVRPPTPVPSPPIRP 677

Query: 316 PSPIKERKMLVDVADYPGTGANVPGGHPPQKPPSPIKERKMLVDVADYPGTGANVPGGHP 375
           P+P+    ++      P   ++ P   PP  PP+PI            P    + P   P
Sbjct: 678 PTPVPSPPIMSPPIKPPTPASSPPMLSPPVTPPTPIPSPPTASPPVTPPTPMPSPPTASP 727

Query: 376 PQKPPSPI 378
           P KPP+P+
Sbjct: 738 PIKPPTPV 727

BLAST of Lag0030081 vs. ExPASy TrEMBL
Match: A0A3P6CLU8 (Uncharacterized protein OS=Brassica campestris OX=3711 GN=BRAA04T17663Z PE=4 SV=1)

HSP 1 Score: 101.7 bits (252), Expect = 7.5e-18
Identity = 114/397 (28.72%), Postives = 146/397 (36.78%), Query Frame = 0

Query: 20  PLQKPPSPIKERKMLVDVADYPGTGA-NVPGGHPPQKPPSPIKERKMLVDVADYPGTGA- 79
           P+QKPP+PI    +       P T   + P   PP KPP+P     +       P T   
Sbjct: 408 PIQKPPTPIYSPPVKPPPVQKPPTPTYSPPVKPPPVKPPTPTYSPPIKPPPVQQPPTPTY 467

Query: 80  NVPGGHPPQKPPSPIKERKMLVDVADYPGTGA-NVPGGHPPHKPPSPIKERKMLVDVADY 139
           + P   PP KPP+PI    +       P T   + P   PP KPP+P     +       
Sbjct: 468 SPPIKPPPVKPPTPIYSPPVKPPPVQKPPTPTYSPPVKPPPVKPPAPTYSPPVKPPPVQK 527

Query: 140 PGTGANVPGGHPP--QKPLSPIKERKMLVDVADYPGTGANVPGGHPP--QKPPSTIKERK 199
           P T    P   PP  QKP +P     +       P T    P   PP  QKPP+      
Sbjct: 528 PPTPTYSPPVKPPPVQKPPTPTYSPPIKPPPVQKPPTPTYSPPIKPPPVQKPPTPTYSPP 587

Query: 200 MLVDVADYPGTGA-NVPGGHPPQKPPSTIKERKMLVDVADYPGTGANVPGGHPPQKPPST 259
           +       P T   + P   PP KPP+ I    +       P T    P   PP KPP  
Sbjct: 588 IKPPPVQKPPTPTYSPPVKPPPVKPPTPIYSPPVKPPPVQQPPT----PSYSPPVKPPPV 647

Query: 260 RKMLVDVADYPSTGANVPGGHPP--QKPPSPIKERKMLVDVADYPGTGANVPGGHPP--Q 319
           +K        P T    P   PP  QKPP+P     +       P T    P   PP  Q
Sbjct: 648 QK--------PPTPTYSPPVKPPPVQKPPTPTYSPPIKPPPVQKPPTPTYSPPIKPPPVQ 707

Query: 320 KPPSPIKERKMLVDVADYPGTGA-NVPGGHPPQKPPSPIKERKMLVDVADYPGTGA-NVP 379
           +PP+P     +       P T   + P   PP KPP+P     +       P T   + P
Sbjct: 708 QPPTPTYSPPVKPPPVQKPPTPTYSPPIKPPPVKPPTPTYSPPIKPPPVQQPPTPTYSPP 767

Query: 380 GGHPPQKPPSPIKVRKMLVDVADYPGTGANVPGGHPP 403
              PP KPP+PI    ++      P T    P   PP
Sbjct: 768 VKPPPVKPPTPIYSPPVIPPPIQKPPTPTYSPPVLPP 792

BLAST of Lag0030081 vs. TAIR 10
Match: AT2G27380.1 (extensin proline-rich 1 )

HSP 1 Score: 96.7 bits (239), Expect = 4.6e-20
Identity = 113/399 (28.32%), Postives = 143/399 (35.84%), Query Frame = 0

Query: 20  PLQKPPSPIKERKMLVDVADYPGTGANVPGGHPP--QKPPSPIKERKMLVDVADYPGTGA 79
           P+QKPP+P     +       P T    P  +PP  QKPP+P     +       P T +
Sbjct: 86  PIQKPPTPTYSPPIYPPPIQKPPTPTYSPPIYPPPIQKPPTPTYSPPIYPPPIQKPPTPS 145

Query: 80  NVPGGHPP--QKPPSPIKERKMLVDVADYPGTGANVPGGHPP-HKPPSPIKERKMLVDVA 139
             P   PP  Q PP+P     +       P T    P   PP HKPP+PI    +     
Sbjct: 146 YSPPVKPPPVQMPPTPTYSPPIKPPPVHKPPTPTYSPPIKPPVHKPPTPIYSPPIKPPPV 205

Query: 140 DYPGTGANVPGGHPP--QKPLSPIKERKMLVDVADYPGTGANVPGGHPP--QKPPSTIKE 199
             P T    P   PP   KP +P     +       P T    P   PP   KPP+ I  
Sbjct: 206 HKPPTPIYSPPIKPPPVHKPPTPTYSPPVKPPPVHKPPTPIYSPPIKPPPVHKPPTPIYS 265

Query: 200 RKMLVDVADYPGTGANVPGGHPP--QKPPSTIKERKMLVDVADYPGTGANVPGGHPPQKP 259
             +       P T    P   PP   KPP+      +       P T    P   PP KP
Sbjct: 266 PPVKPPPVQTPPTPIYSPPVKPPPVHKPPTPTYSPPVKSPPVQKPPT----PTYSPPIKP 325

Query: 260 PSTRKMLVDVADYPSTGANVPGGHPPQKPPSPIKERKMLVDVADYPGTGANVPGGHPP-- 319
           P  +K        P+   + P   PP KPP+PI    +       P T    P   PP  
Sbjct: 326 PPVQKP-------PTPTYSPPIKPPPVKPPTPIYSPPVKPPPVHKPPTPIYSPPVKPPPV 385

Query: 320 QKPPSPIKERKMLVDVADYPGTGANVPGGHPP--QKPPSPIKERKMLVDVADYPGTGANV 379
            KPP+PI    +       P T    P   PP  QKPP+P     + +     P    + 
Sbjct: 386 HKPPTPIYSPPVKPPPIQKPPTPTYSPPIKPPPLQKPPTPTYSPPIKLPPVKPPTPIYSP 445

Query: 380 PGGHPP-QKPPSPIKVRKMLVDVADYPGTGANVPGGHPP 403
           P   PP  KPP+PI    +       P T    P   PP
Sbjct: 446 PVKPPPVHKPPTPIYSPPVKPPPVHKPPTPTYSPPIKPP 473