Lag0030038 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0030038
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	Procollagen-proline 4-dioxygenase
Location	chr8: 43998116 .. 44001397 (-)
RNA-Seq Expression	Lag0030038
Synteny	Lag0030038

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATTCCCGACGGTTCCTCGCATTGTCTCTTTGCTCTCTGTTCCTGTCTGCTGCCTTCGCTCGCTTGCCGGAAACGCGTACGCACAAGAAATTGTACGATCCTTTCCCTCTTCTTCTTGCCCTTCTTTTTCGTTTCGCCAATCCACTGACTCGTGATTTTGGATTCAATTGTTTTTCGAATTTCAGGAGTGGATCTGTGATACGAATGAAGGGGGATCCAGCTCCGTTGATTTTCGATCCTACAAGAGTCACTCAGCTCTCTTGGCGACCCAGGTCATCTTTCTTTGGGGATCTAAATAAACGAATACGATCTTCCTTTACTCTCTGAGAAATGGCTTGATCATGTGTAAAGAAAATCCTACTTCAAATAGTCAAACCGCCTTTTCAATCAGTTGGTTTTTTAATATTATCCTTAAGTGGCGATTTGTTTGTTTATATTCTTCTGAATAGTGTAAATCTACAGGGCATTTTTGTATAAGGGATTTTTATCTGATAAGGAATGTGATCATCTAATCGATCTGGTGAGTGAATTATGGAACGCTTTGTTTGTTTCAATTTAGATTTCGATATTGTGTTAATTGTATATGTTTGTTTGATTCTATTATTTTCGGGAATAGGCCAAGGATAAATTAGAGAAGTCAATGGTAGCAGATAATGAGTCCGGTAAGAGTGTAAGTAGTGAAGTCCGGACGAGTTCTGGCATGTTCCTTCATATGGCCCAGGTGCGTCAGTTCATTTGAAACTTCTTTTTTGAGTTGGACTTGTTAACTTTATTATTATTCTTTGCAAATCGCAACAACGCCTGGATCATTATAATGACTGACTTTTTGCTTAATGATTTTTATGGTATAGTGTGTAAAATATTATGGTGTCATGGTATGTGCTCTTAGAAGTACGTGAGAAGGATAATGTAATTGTGGATGTCATTGTATGGAACTATGATTTTATGAATGTGTTATCCAATATCCTGGTGGTTGATAAACATTCTAAGAAAAAAAAAACTTAAGAAACTATGTGAACTTATATGAATTATATGAATACGTGAATTTTTCAAAATGTGTGTGGTAATGTACGTAATGTTTGTTTAATACAGAAAGAGATGTTTGAATATAAATGAAAATTTTTCTTTTTCTTTTTTTTTGGAGTCTAGTCATTGCCCTAATGTAGTTGGCATTGTTTTGGAGGAGAATTATCAACATCTCTGTTCATTAACCTGTTGTCAACAATATCCCTTGTCTATATGTCATGAGGAAGGATCCGTCTCCCCTCTCTCCTCATATGAATCTATACTGTTGGTGTTGGATGTTCAACTTAATGATGTTGTTGGCAACATCCTTAACTTCTTTTTCCTTTGAAATGGGTGCTTAATCCTGATATTTGTTTCCCCCCAGTTTTCCTCTTTAAATTAAATATGATTCTGTTAGGGAGAGATCGTTGACAGAACCTGCAGATCTCCATTTAGAAGAGTAGTAGGGTGACAGTTCATTGTTATTGATAATCTCAGGTGTTTGAGGACCTAGTTTCTGTGTGTCTTTAGGGAACATGATTTCCTTCATGAATAAAACTTGAGTAGAAGATGTATCGAGGTTAAGTCTATACATCTAGTGAAATGCCGAAAGAGCAATACTAATTTTTATATTCTTTAAAAATATAGTAGTTCAGTTATGTGTTTAATGCCATTACACAGAAAGTTGGTGAATTATGACCCATTACTAATTGCTCCACCAAGTTTAAAGAAACATGGATGACAAAAATTAAAGAATAAGTTGATTCTATTAGTGATAATAATGATGATATTGCGAATGATATATAGAATATATTTAAGAAAGTGACTGTTAGATGGGAATGTTTTACACATCAATACTTGAATATTTTAATCATGGCTTATGCCTCAAATCACCTTGAAGCTTTGATTATAATATGTATATGATCTGTTTTGTGCTTTGAAGGATGAAATTGTTGCTGCCATTGAGGCCAGGATTGCTGCATGGACATTCCTTCCAGCAGGTATATTTGTTGTATGCTCATTGTCATTGATGTGCTCTTTCGTTATTTAGATTATAAAGCTATTACAATCACACTTACCTTTCTTTCTTTAACCTATTATTAAATTCAGAAAACGGAGAGTCCATTCAAATACTGCACTATGAGAATGGTCAAAAGTATGAACCGCATTTTGATTTTTTTCATGACAAGGTGAATCAGGAGTTAGGTGGCCATCGAATAGCTACAGTCTTGATGTATTTATCCAATGTTGAAAAGGGTGGAGAAACCATCTTTCCAAATTCAGAGGTATGGCAGTGGTTCTGCCCCTTAAGTCTGCCTTTGTTTTTCTAGAAACGAAAGTAAATTTTCTTCTTGTGGCCAACTGTGATTACTTTGTTTTCCTGCAGTTCAAAGAATCTCAAGAAAAGGATGACAGTTTTTCTGCTTGTGCTCAAAAGGGTTATGCAGGTAGGTATTTGTTATTGATGCATTATTATGAGTATAGATCATGAGTCATTAATGTATTAGGTTACATTGTCACATAAGGAAAGGCCACGGATGGTCTATGACTCTATGTATCACTGCTGGTGCTGGACCTTCCCTGAAAGTTTCTTTCTGAACTTGATCTTCTTTTCTGGGCTTTGAGAAAGGGTACTCATCGCTAAGTGGCTGACTAATATACTACCTGATCGAGAGCCAACTTGAGAATCAGTGTCATTTTCAATATGTTTTTCATTCTTCTGATACATTTGACGCTGTTATGAGATTTTCACCTTTCCTCACCTTTCCTCGCCGAGACTGCTGTTTGTGACTCTGCATATTTTAACTTAACATTGTTTTTTGGATGTTATATCTCTAAACAACTACTTAAATAAGACCCATTTCGTAATTTTTTCATTATGAGACTCTATTCTATTTTTTTGTCCATGTTTCTAAGCTTATGGGATTGATATTATACGTTCCTCATGGTTCTACAACTTGCCTGTTTGTTGCAGTTAAAGCGAAGAAGGGTGATGCATTGCTGTTCTTCAGCCTCCATCTCGATGCATCGACAGATACCAAAAGCTTGCACGGTAGTTGCCCTGTGATCGAGGGCGAGAAATGGTCTGCAACCAAGTGGATTCATGTGAGATCCTTCGAGAAGCCGACTCGTGCAAGTAGTGAGCGTTGCGTGGACGAAAATGAAAATTGCCCTGCGTGGGCCAAAAGGGGTGAGTGCAAGAAGAACCCTACTTACATGGTGGGTTCTGAAGGTGCTTTAGGATACTGTAGGAAGAGTTGTAAAGCGTGTTAA

mRNA sequence

ATGGATTCCCGACGGTTCCTCGCATTGTCTCTTTGCTCTCTGTTCCTGTCTGCTGCCTTCGCTCGCTTGCCGGAAACGCGTACGCACAAGAAATTGTACGATCCTTTCCCTCTTCTTCTTGCCCTTCTTTTTCGTTTCGCCAATCCACTGACTCGTGATTTTGGATTCAATTGTTTTTCGAATTTCAGGAGTGGATCTGTGATACGAATGAAGGGGGATCCAGCTCCGTTGATTTTCGATCCTACAAGAGTCACTCAGCTCTCTTGGCGACCCAGGGCATTTTTGTATAAGGGATTTTTATCTGATAAGGAATGTGATCATCTAATCGATCTGGCCAAGGATAAATTAGAGAAGTCAATGGTAGCAGATAATGAGTCCGGTAAGAGTGTAAGTAGTGAAGTCCGGACGAGTTCTGGCATGTTCCTTCATATGGCCCAGGATGAAATTGTTGCTGCCATTGAGGCCAGGATTGCTGCATGGACATTCCTTCCAGCAGAAAACGGAGAGTCCATTCAAATACTGCACTATGAGAATGGTCAAAAGTATGAACCGCATTTTGATTTTTTTCATGACAAGGTGAATCAGGAGTTAGGTGGCCATCGAATAGCTACAGTCTTGATGTATTTATCCAATGTTGAAAAGGGTGGAGAAACCATCTTTCCAAATTCAGAGTTCAAAGAATCTCAAGAAAAGGATGACAGTTTTTCTGCTTGTGCTCAAAAGGGTTATGCAGTTAAAGCGAAGAAGGGTGATGCATTGCTGTTCTTCAGCCTCCATCTCGATGCATCGACAGATACCAAAAGCTTGCACGGTAGTTGCCCTGTGATCGAGGGCGAGAAATGGTCTGCAACCAAGTGGATTCATGTGAGATCCTTCGAGAAGCCGACTCGTGCAAGTAGTGAGCGTTGCGTGGACGAAAATGAAAATTGCCCTGCGTGGGCCAAAAGGGGTGAGTGCAAGAAGAACCCTACTTACATGGTGGGTTCTGAAGGTGCTTTAGGATACTGTAGGAAGAGTTGTAAAGCGTGTTAA

Coding sequence (CDS)

ATGGATTCCCGACGGTTCCTCGCATTGTCTCTTTGCTCTCTGTTCCTGTCTGCTGCCTTCGCTCGCTTGCCGGAAACGCGTACGCACAAGAAATTGTACGATCCTTTCCCTCTTCTTCTTGCCCTTCTTTTTCGTTTCGCCAATCCACTGACTCGTGATTTTGGATTCAATTGTTTTTCGAATTTCAGGAGTGGATCTGTGATACGAATGAAGGGGGATCCAGCTCCGTTGATTTTCGATCCTACAAGAGTCACTCAGCTCTCTTGGCGACCCAGGGCATTTTTGTATAAGGGATTTTTATCTGATAAGGAATGTGATCATCTAATCGATCTGGCCAAGGATAAATTAGAGAAGTCAATGGTAGCAGATAATGAGTCCGGTAAGAGTGTAAGTAGTGAAGTCCGGACGAGTTCTGGCATGTTCCTTCATATGGCCCAGGATGAAATTGTTGCTGCCATTGAGGCCAGGATTGCTGCATGGACATTCCTTCCAGCAGAAAACGGAGAGTCCATTCAAATACTGCACTATGAGAATGGTCAAAAGTATGAACCGCATTTTGATTTTTTTCATGACAAGGTGAATCAGGAGTTAGGTGGCCATCGAATAGCTACAGTCTTGATGTATTTATCCAATGTTGAAAAGGGTGGAGAAACCATCTTTCCAAATTCAGAGTTCAAAGAATCTCAAGAAAAGGATGACAGTTTTTCTGCTTGTGCTCAAAAGGGTTATGCAGTTAAAGCGAAGAAGGGTGATGCATTGCTGTTCTTCAGCCTCCATCTCGATGCATCGACAGATACCAAAAGCTTGCACGGTAGTTGCCCTGTGATCGAGGGCGAGAAATGGTCTGCAACCAAGTGGATTCATGTGAGATCCTTCGAGAAGCCGACTCGTGCAAGTAGTGAGCGTTGCGTGGACGAAAATGAAAATTGCCCTGCGTGGGCCAAAAGGGGTGAGTGCAAGAAGAACCCTACTTACATGGTGGGTTCTGAAGGTGCTTTAGGATACTGTAGGAAGAGTTGTAAAGCGTGTTAA

Protein sequence

MDSRRFLALSLCSLFLSAAFARLPETRTHKKLYDPFPLLLALLFRFANPLTRDFGFNCFSNFRSGSVIRMKGDPAPLIFDPTRVTQLSWRPRAFLYKGFLSDKECDHLIDLAKDKLEKSMVADNESGKSVSSEVRTSSGMFLHMAQDEIVAAIEARIAAWTFLPAENGESIQILHYENGQKYEPHFDFFHDKVNQELGGHRIATVLMYLSNVEKGGETIFPNSEFKESQEKDDSFSACAQKGYAVKAKKGDALLFFSLHLDASTDTKSLHGSCPVIEGEKWSATKWIHVRSFEKPTRASSERCVDENENCPAWAKRGECKKNPTYMVGSEGALGYCRKSCKAC

Homology

[Close]

BLAST of Lag0030038 vs. NCBI nr
Match: XP_022134044.1 (probable prolyl 4-hydroxylase 7 [Momordica charantia])

HSP 1 Score: 558.9 bits (1439), Expect = 3.0e-155
Identity = 277/344 (80.52%), Postives = 294/344 (85.47%), Query Frame = 0


Query: 1   MDSRRFLALSLCSLFLSAAFARLPETRTHKKLYDPFPLLLALLFRFANPLTRDFGFNCFS 60
           MDS RFL+ SLC LF+  A ARLP+ R HKK+                            
Sbjct: 1   MDSPRFLSFSLCFLFVFTALARLPDMRAHKKI---------------------------- 60

Query: 61  NFRSGSVIRMKGDPAPLIFDPTRVTQLSWRPRAFLYKGFLSDKECDHLIDLAKDKLEKSM 120
              SGSV+R+KG+P+PLIFDPTRVTQLSW+PRAFLYKGFLSDKECDHLIDLAKDKLEKSM
Sbjct: 61  ---SGSVLRLKGEPSPLIFDPTRVTQLSWQPRAFLYKGFLSDKECDHLIDLAKDKLEKSM 120

Query: 121 VADNESGKSVSSEVRTSSGMFLHMAQDEIVAAIEARIAAWTFLPAENGESIQILHYENGQ 180
           VADN SGKSVSSEVRTSSGMFLH AQDEIVAA+EARIAAWTFLPAENGESIQILHYENGQ
Sbjct: 121 VADNNSGKSVSSEVRTSSGMFLHKAQDEIVAAVEARIAAWTFLPAENGESIQILHYENGQ 180

Query: 181 KYEPHFDFFHDKVNQELGGHRIATVLMYLSNVEKGGETIFPNSEFKESQEKDDSFSACAQ 240
           KYEPHFD+FHDKVNQELGGHR+ATVLMYLSNVEKGGETIFPNSEFKESQEKDDS+S CA+
Sbjct: 181 KYEPHFDYFHDKVNQELGGHRVATVLMYLSNVEKGGETIFPNSEFKESQEKDDSWSDCAR 240

Query: 241 KGYAVKAKKGDALLFFSLHLDASTDTKSLHGSCPVIEGEKWSATKWIHVRSFEKPTRASS 300
           KGYAVKAKKGDALLFFSLHLDA+TD KSLHGSCPVIEGEKWSATKWIHVRSFEKPTR S 
Sbjct: 241 KGYAVKAKKGDALLFFSLHLDATTDVKSLHGSCPVIEGEKWSATKWIHVRSFEKPTRPSR 300

Query: 301 E-RCVDENENCPAWAKRGECKKNPTYMVGSEGALGYCRKSCKAC 344
              CVDENENC +WAKRGECKKNPTYMVGSE ALGYCRKSC+AC
Sbjct: 301 RLDCVDENENCASWAKRGECKKNPTYMVGSESALGYCRKSCQAC 313

[Close]

BLAST of Lag0030038 vs. NCBI nr
Match: XP_008458700.1 (PREDICTED: probable prolyl 4-hydroxylase 7 [Cucumis melo])

HSP 1 Score: 548.9 bits (1413), Expect = 3.1e-152
Identity = 274/343 (79.88%), Postives = 290/343 (84.55%), Query Frame = 0


Query: 1   MDSRRFLALSLCSLFLSAAFARLPETRTHKKLYDPFPLLLALLFRFANPLTRDFGFNCFS 60
           MDSR FLA SLC L +  AFARLPETR  K  Y                           
Sbjct: 1   MDSRPFLAFSLCFLSVFTAFARLPETRMLKHSYKQ------------------------- 60

Query: 61  NFRSGSVIRMKGDPAPLIFDPTRVTQLSWRPRAFLYKGFLSDKECDHLIDLAKDKLEKSM 120
              +GSV+R+K D +PLIFDPTRVTQLSW+PRAFLYKGFLSD+ECDHLIDLAKDKLEKSM
Sbjct: 61  --STGSVLRLKTDSSPLIFDPTRVTQLSWQPRAFLYKGFLSDEECDHLIDLAKDKLEKSM 120

Query: 121 VADNESGKSVSSEVRTSSGMFLHMAQDEIVAAIEARIAAWTFLPAENGESIQILHYENGQ 180
           VADNESGKSVSSEVRTSSGMFL  AQD+IVA +EARIAAWT LPAENGESIQILHYENGQ
Sbjct: 121 VADNESGKSVSSEVRTSSGMFLRKAQDKIVAGVEARIAAWTLLPAENGESIQILHYENGQ 180

Query: 181 KYEPHFDFFHDKVNQELGGHRIATVLMYLSNVEKGGETIFPNSEFKESQEKDDSFSACAQ 240
           KYEPHFDFFHDKVNQELGGHRIATVLMYLSNVEKGGETIFPNSEFKESQEKDDS+S C++
Sbjct: 181 KYEPHFDFFHDKVNQELGGHRIATVLMYLSNVEKGGETIFPNSEFKESQEKDDSWSDCSR 240

Query: 241 KGYAVKAKKGDALLFFSLHLDASTDTKSLHGSCPVIEGEKWSATKWIHVRSFEKPTRASS 300
           KGYAVKA+KGDALLFFSLHLDA+TD +SLHGSCPVIEGEKWSATKWIHVRSFEK  R S 
Sbjct: 241 KGYAVKAQKGDALLFFSLHLDATTDERSLHGSCPVIEGEKWSATKWIHVRSFEKLPRVSR 300

Query: 301 ERCVDENENCPAWAKRGECKKNPTYMVGSEGALGYCRKSCKAC 344
           + CVDENENCPAWAKRGECKKNPTYMVGSEGALGYCRKSCKAC
Sbjct: 301 QDCVDENENCPAWAKRGECKKNPTYMVGSEGALGYCRKSCKAC 316

[Close]

BLAST of Lag0030038 vs. NCBI nr
Match: XP_038889686.1 (probable prolyl 4-hydroxylase 7 isoform X1 [Benincasa hispida])

HSP 1 Score: 542.3 bits (1396), Expect = 2.9e-150
Identity = 272/343 (79.30%), Postives = 285/343 (83.09%), Query Frame = 0


Query: 1   MDSRRFLALSLCSLFLSAAFARLPETRTHKKLYDPFPLLLALLFRFANPLTRDFGFNCFS 60
           MDSRRFLA  LC L +   FARLPE R+ KK                             
Sbjct: 1   MDSRRFLAFCLCFLSVFTGFARLPELRSQKK----------------------------- 60

Query: 61  NFRSGSVIRMKGDPAPLIFDPTRVTQLSWRPRAFLYKGFLSDKECDHLIDLAKDKLEKSM 120
              SGSVIR+K D +PL+FDPTRVTQLSW PRAFLYKGFLSDKECDHLIDLAKDKLEKSM
Sbjct: 61  --SSGSVIRLKTDSSPLVFDPTRVTQLSWEPRAFLYKGFLSDKECDHLIDLAKDKLEKSM 120

Query: 121 VADNESGKSVSSEVRTSSGMFLHMAQDEIVAAIEARIAAWTFLPAENGESIQILHYENGQ 180
           VADNESGKSVSSEVRTSSGMFL  AQDEIVAAIEARI+AWT LPAENGESIQILHYENGQ
Sbjct: 121 VADNESGKSVSSEVRTSSGMFLRKAQDEIVAAIEARISAWTLLPAENGESIQILHYENGQ 180

Query: 181 KYEPHFDFFHDKVNQELGGHRIATVLMYLSNVEKGGETIFPNSEFKESQEKDDSFSACAQ 240
           KYEPHFDFFHDKVNQELGGHRIATVLMYLSNVEKGGETIFPNSEFKESQEKD+S+S CA+
Sbjct: 181 KYEPHFDFFHDKVNQELGGHRIATVLMYLSNVEKGGETIFPNSEFKESQEKDESWSDCAR 240

Query: 241 KGYAVKAKKGDALLFFSLHLDASTDTKSLHGSCPVIEGEKWSATKWIHVRSFEKPTRASS 300
           KGYAVKA+KGDALLFFSL  DA+TD KSLHGSCPVIEGEKWSATKWIHVRSFEK TR S 
Sbjct: 241 KGYAVKARKGDALLFFSLRPDATTDVKSLHGSCPVIEGEKWSATKWIHVRSFEKATRVSR 300

Query: 301 ERCVDENENCPAWAKRGECKKNPTYMVGSEGALGYCRKSCKAC 344
           + CVDENENC  WAKRGECKKNPTYMVGSE ALGYCRKSC+AC
Sbjct: 301 QDCVDENENCQIWAKRGECKKNPTYMVGSEDALGYCRKSCRAC 312

[Close]

BLAST of Lag0030038 vs. NCBI nr
Match: XP_038889687.1 (probable prolyl 4-hydroxylase 7 isoform X2 [Benincasa hispida])

HSP 1 Score: 542.0 bits (1395), Expect = 3.8e-150
Identity = 272/343 (79.30%), Postives = 285/343 (83.09%), Query Frame = 0


Query: 1   MDSRRFLALSLCSLFLSAAFARLPETRTHKKLYDPFPLLLALLFRFANPLTRDFGFNCFS 60
           MDSRRFLA  LC L +   FARLPE R+ KK                             
Sbjct: 1   MDSRRFLAFCLCFLSVFTGFARLPELRSQKK----------------------------- 60

Query: 61  NFRSGSVIRMKGDPAPLIFDPTRVTQLSWRPRAFLYKGFLSDKECDHLIDLAKDKLEKSM 120
              SGSVIR+K D +PL+FDPTRVTQLSW PRAFLYKGFLSDKECDHLIDLAKDKLEKSM
Sbjct: 61  ---SGSVIRLKTDSSPLVFDPTRVTQLSWEPRAFLYKGFLSDKECDHLIDLAKDKLEKSM 120

Query: 121 VADNESGKSVSSEVRTSSGMFLHMAQDEIVAAIEARIAAWTFLPAENGESIQILHYENGQ 180
           VADNESGKSVSSEVRTSSGMFL  AQDEIVAAIEARI+AWT LPAENGESIQILHYENGQ
Sbjct: 121 VADNESGKSVSSEVRTSSGMFLRKAQDEIVAAIEARISAWTLLPAENGESIQILHYENGQ 180

Query: 181 KYEPHFDFFHDKVNQELGGHRIATVLMYLSNVEKGGETIFPNSEFKESQEKDDSFSACAQ 240
           KYEPHFDFFHDKVNQELGGHRIATVLMYLSNVEKGGETIFPNSEFKESQEKD+S+S CA+
Sbjct: 181 KYEPHFDFFHDKVNQELGGHRIATVLMYLSNVEKGGETIFPNSEFKESQEKDESWSDCAR 240

Query: 241 KGYAVKAKKGDALLFFSLHLDASTDTKSLHGSCPVIEGEKWSATKWIHVRSFEKPTRASS 300
           KGYAVKA+KGDALLFFSL  DA+TD KSLHGSCPVIEGEKWSATKWIHVRSFEK TR S 
Sbjct: 241 KGYAVKARKGDALLFFSLRPDATTDVKSLHGSCPVIEGEKWSATKWIHVRSFEKATRVSR 300

Query: 301 ERCVDENENCPAWAKRGECKKNPTYMVGSEGALGYCRKSCKAC 344
           + CVDENENC  WAKRGECKKNPTYMVGSE ALGYCRKSC+AC
Sbjct: 301 QDCVDENENCQIWAKRGECKKNPTYMVGSEDALGYCRKSCRAC 311

[Close]

BLAST of Lag0030038 vs. NCBI nr
Match: XP_011655982.1 (probable prolyl 4-hydroxylase 7 isoform X1 [Cucumis sativus])

HSP 1 Score: 535.0 bits (1377), Expect = 4.6e-148
Identity = 269/344 (78.20%), Postives = 288/344 (83.72%), Query Frame = 0


Query: 1   MDSRRFLALSLCSLFLSAAFARLPETRTHKKLYDPFPLLLALLFRFANPLTRDFGFNCFS 60
           MDSR FLA SLC L +  AFARLPETRTHK+                             
Sbjct: 1   MDSRPFLAFSLCFLSVFTAFARLPETRTHKQ----------------------------- 60

Query: 61  NFRSGSVIRMKGDPAPLIFDPTRVTQLSWRPRAFLYKGFLSDKECDHLIDLAKDKLEKSM 120
              SGSV+R+K D +PLIFDPTRVTQLSW+PRAFLYKGFLSD ECDHLIDLAKDKLEKSM
Sbjct: 61  --SSGSVLRLKTDSSPLIFDPTRVTQLSWQPRAFLYKGFLSDAECDHLIDLAKDKLEKSM 120

Query: 121 VADNESGKSVSSEVRTSSGMFLHMAQDEIVAAIEARIAAWTFLPAENGESIQILHYENGQ 180
           VADN+SGKSVSSEVRTSSGMFL  AQDE+VA +EARIAAWT LPAENGESIQILHYENGQ
Sbjct: 121 VADNDSGKSVSSEVRTSSGMFLRKAQDEVVAGVEARIAAWTLLPAENGESIQILHYENGQ 180

Query: 181 KYEPHFDFFHDKVNQELGGHRIATVLMYLSNVEKGGETIFPNSEFKESQEKDDSFSACAQ 240
           KYEPHFDFFHDKVNQELGGHRIATVLMYLSNVEKGGETIFPNSEFKESQ KD+S+S C++
Sbjct: 181 KYEPHFDFFHDKVNQELGGHRIATVLMYLSNVEKGGETIFPNSEFKESQAKDESWSDCSR 240

Query: 241 KGYAVKAKKGDALLFFSLHLDASTDTKSLHGSCPVIEGEKWSATKWIHVRSFEKPT-RAS 300
           KGYAVKA+KGDALLFFSL+LDA+TD +SLHGSCPVI GEKWSATKWIHVRSFEK T R S
Sbjct: 241 KGYAVKAQKGDALLFFSLNLDATTDERSLHGSCPVIAGEKWSATKWIHVRSFEKITSRVS 300

Query: 301 SERCVDENENCPAWAKRGECKKNPTYMVGSEGALGYCRKSCKAC 344
            + CVDENENC AWAK+GECKKNPTYMVGS GALGYCRKSCKAC
Sbjct: 301 RQGCVDENENCLAWAKKGECKKNPTYMVGSGGALGYCRKSCKAC 313

[Close]

BLAST of Lag0030038 vs. ExPASy Swiss-Prot
Match: Q8L970 (Probable prolyl 4-hydroxylase 7 OS=Arabidopsis thaliana OX=3702 GN=P4H7 PE=2 SV=1)

HSP 1 Score: 440.7 bits (1132), Expect = 1.6e-122
Identity = 225/343 (65.60%), Postives = 261/343 (76.09%), Query Frame = 0


Query: 1   MDSRRFLALSLCSLFLSAAFARLPETRTHKKLYDPFPLLLALLFRFANPLTRDFGFNCFS 60
           MDSR FLA SLC LF                     PL+ +   RF   LTR       S
Sbjct: 1   MDSRIFLAFSLCFLF-------------------TLPLISSAPNRF---LTRS------S 60

Query: 61  NFRSGSVIRMKGDPAPLIFDPTRVTQLSWRPRAFLYKGFLSDKECDHLIDLAKDKLEKSM 120
           N R GSVI+MK   +   FDPTRVTQLSW PR FLY+GFLSD+ECDH I LAK KLEKSM
Sbjct: 61  NTRDGSVIKMKTSASSFGFDPTRVTQLSWTPRVFLYEGFLSDEECDHFIKLAKGKLEKSM 120

Query: 121 VADNESGKSVSSEVRTSSGMFLHMAQDEIVAAIEARIAAWTFLPAENGESIQILHYENGQ 180
           VADN+SG+SV SEVRTSSGMFL   QD+IV+ +EA++AAWTFLP ENGES+QILHYENGQ
Sbjct: 121 VADNDSGESVESEVRTSSGMFLSKRQDDIVSNVEAKLAAWTFLPEENGESMQILHYENGQ 180

Query: 181 KYEPHFDFFHDKVNQELGGHRIATVLMYLSNVEKGGETIFPNSEFKESQEKDDSFSACAQ 240
           KYEPHFD+FHD+ N ELGGHRIATVLMYLSNVEKGGET+FP  + K +Q KDDS++ CA+
Sbjct: 181 KYEPHFDYFHDQANLELGGHRIATVLMYLSNVEKGGETVFPMWKGKATQLKDDSWTECAK 240

Query: 241 KGYAVKAKKGDALLFFSLHLDASTDTKSLHGSCPVIEGEKWSATKWIHVRSFEKPTRASS 300
           +GYAVK +KGDALLFF+LH +A+TD+ SLHGSCPV+EGEKWSAT+WIHV+SFE+     S
Sbjct: 241 QGYAVKPRKGDALLFFNLHPNATTDSNSLHGSCPVVEGEKWSATRWIHVKSFERAFNKQS 300

Query: 301 ERCVDENENCPAWAKRGECKKNPTYMVGSEGALGYCRKSCKAC 344
             C+DEN +C  WAK GEC+KNPTYMVGS+   GYCRKSCKAC
Sbjct: 301 -GCMDENVSCEKWAKAGECQKNPTYMVGSDKDHGYCRKSCKAC 314

[Close]

BLAST of Lag0030038 vs. ExPASy Swiss-Prot
Match: F4J0A8 (Probable prolyl 4-hydroxylase 6 OS=Arabidopsis thaliana OX=3702 GN=P4H6 PE=2 SV=1)

HSP 1 Score: 403.3 bits (1035), Expect = 2.8e-111
Identity = 195/265 (73.58%), Postives = 224/265 (84.53%), Query Frame = 0


Query: 80  DPTRVTQLSWRPRAFLYKGFLSDKECDHLIDLAKDKLEKSM-VADNESGKSVSSEVRTSS 139
           DPTR+TQLSW PRAFLYKGFLSD+ECDHLI LAK KLEKSM VAD +SG+S  SEVRTSS
Sbjct: 28  DPTRITQLSWTPRAFLYKGFLSDEECDHLIKLAKGKLEKSMVVADVDSGESEDSEVRTSS 87

Query: 140 GMFLHMAQDEIVAAIEARIAAWTFLPAENGESIQILHYENGQKYEPHFDFFHDKVNQELG 199
           GMFL   QD+IVA +EA++AAWTFLP ENGE++QILHYENGQKY+PHFD+F+DK   ELG
Sbjct: 88  GMFLTKRQDDIVANVEAKLAAWTFLPEENGEALQILHYENGQKYDPHFDYFYDKKALELG 147

Query: 200 GHRIATVLMYLSNVEKGGETIFPNSEFKESQEKDDSFSACAQKGYAVKAKKGDALLFFSL 259
           GHRIATVLMYLSNV KGGET+FPN + K  Q KDDS+S CA++GYAVK +KGDALLFF+L
Sbjct: 148 GHRIATVLMYLSNVTKGGETVFPNWKGKTPQLKDDSWSKCAKQGYAVKPRKGDALLFFNL 207

Query: 260 HLDASTDTKSLHGSCPVIEGEKWSATKWIHVRSFEKPTRASSERCVDENENCPAWAKRGE 319
           HL+ +TD  SLHGSCPVIEGEKWSAT+WIHVRSF K        CVD++E+C  WA  GE
Sbjct: 208 HLNGTTDPNSLHGSCPVIEGEKWSATRWIHVRSFGKKKLV----CVDDHESCQEWADAGE 267

Query: 320 CKKNPTYMVGSEGALGYCRKSCKAC 344
           C+KNP YMVGSE +LG+CRKSCKAC
Sbjct: 268 CEKNPMYMVGSETSLGFCRKSCKAC 288

[Close]

BLAST of Lag0030038 vs. ExPASy Swiss-Prot
Match: Q8LAN3 (Probable prolyl 4-hydroxylase 4 OS=Arabidopsis thaliana OX=3702 GN=P4H4 PE=2 SV=1)

HSP 1 Score: 339.3 bits (869), Expect = 4.9e-92
Identity = 162/267 (60.67%), Postives = 202/267 (75.66%), Query Frame = 0


Query: 80  DPTRVTQLSWRPRAFLYKGFLSDKECDHLIDLAKDKLEKSMVADNESGKSVSSEVRTSSG 139
           +P++V Q+S +PRAF+Y+GFL++ ECDH++ LAK  L++S VADN+SG+S  SEVRTSSG
Sbjct: 33  NPSKVKQVSSKPRAFVYEGFLTELECDHMVSLAKASLKRSAVADNDSGESKFSEVRTSSG 92

Query: 140 MFLHMAQDEIVAAIEARIAAWTFLPAENGESIQILHYENGQKYEPHFDFFHDKVNQELGG 199
            F+   +D IV+ IE +I+ WTFLP ENGE IQ+L YE+GQKY+ HFD+FHDKVN   GG
Sbjct: 93  TFISKGKDPIVSGIEDKISTWTFLPKENGEDIQVLRYEHGQKYDAHFDYFHDKVNIVRGG 152

Query: 200 HRIATVLMYLSNVEKGGETIFPNSEFKESQ---EKDDSFSACAQKGYAVKAKKGDALLFF 259
           HR+AT+LMYLSNV KGGET+FP++E    +   E  +  S CA++G AVK +KGDALLFF
Sbjct: 153 HRMATILMYLSNVTKGGETVFPDAEIPSRRVLSENKEDLSDCAKRGIAVKPRKGDALLFF 212

Query: 260 SLHLDASTDTKSLHGSCPVIEGEKWSATKWIHVRSFEKPTRASSERCVDENENCPAWAKR 319
           +LH DA  D  SLHG CPVIEGEKWSATKWIHV SF++    S   C D NE+C  WA  
Sbjct: 213 NLHPDAIPDPLSLHGGCPVIEGEKWSATKWIHVDSFDRIVTPSG-NCTDMNESCERWAVL 272

Query: 320 GECKKNPTYMVGSEGALGYCRKSCKAC 344
           GEC KNP YMVG+    GYCR+SCKAC
Sbjct: 273 GECTKNPEYMVGTTELPGYCRRSCKAC 298

[Close]

BLAST of Lag0030038 vs. ExPASy Swiss-Prot
Match: F4JAU3 (Prolyl 4-hydroxylase 2 OS=Arabidopsis thaliana OX=3702 GN=P4H2 PE=1 SV=1)

HSP 1 Score: 335.1 bits (858), Expect = 9.3e-91
Identity = 164/269 (60.97%), Postives = 202/269 (75.09%), Query Frame = 0


Query: 78  IFDPTRVTQLSWRPRAFLYKGFLSDKECDHLIDLAKDKLEKSMVADNESGKSVSSEVRTS 137
           I +P++V Q+S +PRAF+Y+GFL+D ECDHLI LAK+ L++S VADN++G+S  S+VRTS
Sbjct: 32  IINPSKVKQVSSKPRAFVYEGFLTDLECDHLISLAKENLQRSAVADNDNGESQVSDVRTS 91

Query: 138 SGMFLHMAQDEIVAAIEARIAAWTFLPAENGESIQILHYENGQKYEPHFDFFHDKVNQEL 197
           SG F+   +D IV+ IE +++ WTFLP ENGE +Q+L YE+GQKY+ HFD+FHDKVN   
Sbjct: 92  SGTFISKGKDPIVSGIEDKLSTWTFLPKENGEDLQVLRYEHGQKYDAHFDYFHDKVNIAR 151

Query: 198 GGHRIATVLMYLSNVEKGGETIFPNS-EF--KESQEKDDSFSACAQKGYAVKAKKGDALL 257
           GGHRIATVL+YLSNV KGGET+FP++ EF  +   E  D  S CA+KG AVK KKG+ALL
Sbjct: 152 GGHRIATVLLYLSNVTKGGETVFPDAQEFSRRSLSENKDDLSDCAKKGIAVKPKKGNALL 211

Query: 258 FFSLHLDASTDTKSLHGSCPVIEGEKWSATKWIHVRSFEKPTRASSERCVDENENCPAWA 317
           FF+L  DA  D  SLHG CPVIEGEKWSATKWIHV SF+K        C D NE+C  WA
Sbjct: 212 FFNLQQDAIPDPFSLHGGCPVIEGEKWSATKWIHVDSFDK-ILTHDGNCTDVNESCERWA 271

Query: 318 KRGECKKNPTYMVGSEGALGYCRKSCKAC 344
             GEC KNP YMVG+    G CR+SCKAC
Sbjct: 272 VLGECGKNPEYMVGTPEIPGNCRRSCKAC 299

[Close]

BLAST of Lag0030038 vs. ExPASy Swiss-Prot
Match: Q9LN20 (Probable prolyl 4-hydroxylase 3 OS=Arabidopsis thaliana OX=3702 GN=P4H3 PE=2 SV=1)

HSP 1 Score: 243.0 bits (619), Expect = 4.8e-63
Identity = 116/208 (55.77%), Postives = 153/208 (73.56%), Query Frame = 0


Query: 87  LSWRPRAFLYKGFLSDKECDHLIDLAKDKLEKSMVADNESGKSVSSEVRTSSGMFLHMAQ 146
           LSW PRAF+Y  FLS +EC++LI LAK  + KS V D+E+GKS  S VRTSSG FL   +
Sbjct: 79  LSWEPRAFVYHNFLSKEECEYLISLAKPHMVKSTVVDSETGKSKDSRVRTSSGTFLRRGR 138

Query: 147 DEIVAAIEARIAAWTFLPAENGESIQILHYENGQKYEPHFDFFHDKVNQELGGHRIATVL 206
           D+I+  IE RIA +TF+PA++GE +Q+LHYE GQKYEPH+D+F D+ N + GG R+AT+L
Sbjct: 139 DKIIKTIEKRIADYTFIPADHGEGLQVLHYEAGQKYEPHYDYFVDEFNTKNGGQRMATML 198

Query: 207 MYLSNVEKGGETIFPNSEFK-ESQEKDDSFSACAQKGYAVKAKKGDALLFFSLHLDASTD 266
           MYLS+VE+GGET+FP +     S    +  S C +KG +VK + GDALLF+S+  DA+ D
Sbjct: 199 MYLSDVEEGGETVFPAANMNFSSVPWYNELSECGKKGLSVKPRMGDALLFWSMRPDATLD 258

Query: 267 TKSLHGSCPVIEGEKWSATKWIHVRSFE 294
             SLHG CPVI G KWS+TKW+HV  ++
Sbjct: 259 PTSLHGGCPVIRGNKWSSTKWMHVGEYK 286

[Close]

BLAST of Lag0030038 vs. ExPASy TrEMBL
Match: A0A6J1BXN9 (Procollagen-proline 4-dioxygenase OS=Momordica charantia OX=3673 GN=LOC111006412 PE=3 SV=1)

HSP 1 Score: 558.9 bits (1439), Expect = 1.5e-155
Identity = 277/344 (80.52%), Postives = 294/344 (85.47%), Query Frame = 0


Query: 1   MDSRRFLALSLCSLFLSAAFARLPETRTHKKLYDPFPLLLALLFRFANPLTRDFGFNCFS 60
           MDS RFL+ SLC LF+  A ARLP+ R HKK+                            
Sbjct: 1   MDSPRFLSFSLCFLFVFTALARLPDMRAHKKI---------------------------- 60

Query: 61  NFRSGSVIRMKGDPAPLIFDPTRVTQLSWRPRAFLYKGFLSDKECDHLIDLAKDKLEKSM 120
              SGSV+R+KG+P+PLIFDPTRVTQLSW+PRAFLYKGFLSDKECDHLIDLAKDKLEKSM
Sbjct: 61  ---SGSVLRLKGEPSPLIFDPTRVTQLSWQPRAFLYKGFLSDKECDHLIDLAKDKLEKSM 120

Query: 121 VADNESGKSVSSEVRTSSGMFLHMAQDEIVAAIEARIAAWTFLPAENGESIQILHYENGQ 180
           VADN SGKSVSSEVRTSSGMFLH AQDEIVAA+EARIAAWTFLPAENGESIQILHYENGQ
Sbjct: 121 VADNNSGKSVSSEVRTSSGMFLHKAQDEIVAAVEARIAAWTFLPAENGESIQILHYENGQ 180

Query: 181 KYEPHFDFFHDKVNQELGGHRIATVLMYLSNVEKGGETIFPNSEFKESQEKDDSFSACAQ 240
           KYEPHFD+FHDKVNQELGGHR+ATVLMYLSNVEKGGETIFPNSEFKESQEKDDS+S CA+
Sbjct: 181 KYEPHFDYFHDKVNQELGGHRVATVLMYLSNVEKGGETIFPNSEFKESQEKDDSWSDCAR 240

Query: 241 KGYAVKAKKGDALLFFSLHLDASTDTKSLHGSCPVIEGEKWSATKWIHVRSFEKPTRASS 300
           KGYAVKAKKGDALLFFSLHLDA+TD KSLHGSCPVIEGEKWSATKWIHVRSFEKPTR S 
Sbjct: 241 KGYAVKAKKGDALLFFSLHLDATTDVKSLHGSCPVIEGEKWSATKWIHVRSFEKPTRPSR 300

Query: 301 E-RCVDENENCPAWAKRGECKKNPTYMVGSEGALGYCRKSCKAC 344
              CVDENENC +WAKRGECKKNPTYMVGSE ALGYCRKSC+AC
Sbjct: 301 RLDCVDENENCASWAKRGECKKNPTYMVGSESALGYCRKSCQAC 313

[Close]

BLAST of Lag0030038 vs. ExPASy TrEMBL
Match: A0A1S3C8G4 (Procollagen-proline 4-dioxygenase OS=Cucumis melo OX=3656 GN=LOC103498028 PE=3 SV=1)

HSP 1 Score: 548.9 bits (1413), Expect = 1.5e-152
Identity = 274/343 (79.88%), Postives = 290/343 (84.55%), Query Frame = 0


Query: 1   MDSRRFLALSLCSLFLSAAFARLPETRTHKKLYDPFPLLLALLFRFANPLTRDFGFNCFS 60
           MDSR FLA SLC L +  AFARLPETR  K  Y                           
Sbjct: 1   MDSRPFLAFSLCFLSVFTAFARLPETRMLKHSYKQ------------------------- 60

Query: 61  NFRSGSVIRMKGDPAPLIFDPTRVTQLSWRPRAFLYKGFLSDKECDHLIDLAKDKLEKSM 120
              +GSV+R+K D +PLIFDPTRVTQLSW+PRAFLYKGFLSD+ECDHLIDLAKDKLEKSM
Sbjct: 61  --STGSVLRLKTDSSPLIFDPTRVTQLSWQPRAFLYKGFLSDEECDHLIDLAKDKLEKSM 120

Query: 121 VADNESGKSVSSEVRTSSGMFLHMAQDEIVAAIEARIAAWTFLPAENGESIQILHYENGQ 180
           VADNESGKSVSSEVRTSSGMFL  AQD+IVA +EARIAAWT LPAENGESIQILHYENGQ
Sbjct: 121 VADNESGKSVSSEVRTSSGMFLRKAQDKIVAGVEARIAAWTLLPAENGESIQILHYENGQ 180

Query: 181 KYEPHFDFFHDKVNQELGGHRIATVLMYLSNVEKGGETIFPNSEFKESQEKDDSFSACAQ 240
           KYEPHFDFFHDKVNQELGGHRIATVLMYLSNVEKGGETIFPNSEFKESQEKDDS+S C++
Sbjct: 181 KYEPHFDFFHDKVNQELGGHRIATVLMYLSNVEKGGETIFPNSEFKESQEKDDSWSDCSR 240

Query: 241 KGYAVKAKKGDALLFFSLHLDASTDTKSLHGSCPVIEGEKWSATKWIHVRSFEKPTRASS 300
           KGYAVKA+KGDALLFFSLHLDA+TD +SLHGSCPVIEGEKWSATKWIHVRSFEK  R S 
Sbjct: 241 KGYAVKAQKGDALLFFSLHLDATTDERSLHGSCPVIEGEKWSATKWIHVRSFEKLPRVSR 300

Query: 301 ERCVDENENCPAWAKRGECKKNPTYMVGSEGALGYCRKSCKAC 344
           + CVDENENCPAWAKRGECKKNPTYMVGSEGALGYCRKSCKAC
Sbjct: 301 QDCVDENENCPAWAKRGECKKNPTYMVGSEGALGYCRKSCKAC 316

[Close]

BLAST of Lag0030038 vs. ExPASy TrEMBL
Match: A0A0A0KS38 (Procollagen-proline 4-dioxygenase OS=Cucumis sativus OX=3659 GN=Csa_5G633280 PE=3 SV=1)

HSP 1 Score: 535.0 bits (1377), Expect = 2.3e-148
Identity = 269/344 (78.20%), Postives = 288/344 (83.72%), Query Frame = 0


Query: 1   MDSRRFLALSLCSLFLSAAFARLPETRTHKKLYDPFPLLLALLFRFANPLTRDFGFNCFS 60
           MDSR FLA SLC L +  AFARLPETRTHK+                             
Sbjct: 1   MDSRPFLAFSLCFLSVFTAFARLPETRTHKQ----------------------------- 60

Query: 61  NFRSGSVIRMKGDPAPLIFDPTRVTQLSWRPRAFLYKGFLSDKECDHLIDLAKDKLEKSM 120
              SGSV+R+K D +PLIFDPTRVTQLSW+PRAFLYKGFLSD ECDHLIDLAKDKLEKSM
Sbjct: 61  --SSGSVLRLKTDSSPLIFDPTRVTQLSWQPRAFLYKGFLSDAECDHLIDLAKDKLEKSM 120

Query: 121 VADNESGKSVSSEVRTSSGMFLHMAQDEIVAAIEARIAAWTFLPAENGESIQILHYENGQ 180
           VADN+SGKSVSSEVRTSSGMFL  AQDE+VA +EARIAAWT LPAENGESIQILHYENGQ
Sbjct: 121 VADNDSGKSVSSEVRTSSGMFLRKAQDEVVAGVEARIAAWTLLPAENGESIQILHYENGQ 180

Query: 181 KYEPHFDFFHDKVNQELGGHRIATVLMYLSNVEKGGETIFPNSEFKESQEKDDSFSACAQ 240
           KYEPHFDFFHDKVNQELGGHRIATVLMYLSNVEKGGETIFPNSEFKESQ KD+S+S C++
Sbjct: 181 KYEPHFDFFHDKVNQELGGHRIATVLMYLSNVEKGGETIFPNSEFKESQAKDESWSDCSR 240

Query: 241 KGYAVKAKKGDALLFFSLHLDASTDTKSLHGSCPVIEGEKWSATKWIHVRSFEKPT-RAS 300
           KGYAVKA+KGDALLFFSL+LDA+TD +SLHGSCPVI GEKWSATKWIHVRSFEK T R S
Sbjct: 241 KGYAVKAQKGDALLFFSLNLDATTDERSLHGSCPVIAGEKWSATKWIHVRSFEKITSRVS 300

Query: 301 SERCVDENENCPAWAKRGECKKNPTYMVGSEGALGYCRKSCKAC 344
            + CVDENENC AWAK+GECKKNPTYMVGS GALGYCRKSCKAC
Sbjct: 301 RQGCVDENENCLAWAKKGECKKNPTYMVGSGGALGYCRKSCKAC 313

[Close]

BLAST of Lag0030038 vs. ExPASy TrEMBL
Match: A0A6J1FJ93 (Procollagen-proline 4-dioxygenase OS=Cucurbita moschata OX=3662 GN=LOC111444767 PE=3 SV=1)

HSP 1 Score: 532.3 bits (1370), Expect = 1.5e-147
Identity = 270/343 (78.72%), Postives = 288/343 (83.97%), Query Frame = 0


Query: 1   MDSRRFLALSLCSLFLSAAFARLPETRTHKKLYDPFPLLLALLFRFANPLTRDFGFNCFS 60
           MDSRRFLA SL  L +S  FARLPE  THKKL                            
Sbjct: 1   MDSRRFLAFSLFFLSVSTGFARLPE--THKKL---------------------------- 60

Query: 61  NFRSGSVIRMKGDPAPLIFDPTRVTQLSWRPRAFLYKGFLSDKECDHLIDLAKDKLEKSM 120
              SGSV+ +K D   LIFDPTRVTQLSW+PRAFLYKGFL+D+ECDHLIDLAKDKLEKSM
Sbjct: 61  ---SGSVLELKRDSPRLIFDPTRVTQLSWQPRAFLYKGFLTDQECDHLIDLAKDKLEKSM 120

Query: 121 VADNESGKSVSSEVRTSSGMFLHMAQDEIVAAIEARIAAWTFLPAENGESIQILHYENGQ 180
           VADNESGKSVSSEVRTSSGMFL  AQDEIVA IEARI+AWTFLP ENGESIQILHYENGQ
Sbjct: 121 VADNESGKSVSSEVRTSSGMFLRKAQDEIVAGIEARISAWTFLPVENGESIQILHYENGQ 180

Query: 181 KYEPHFDFFHDKVNQELGGHRIATVLMYLSNVEKGGETIFPNSEFKESQEKDDSFSACAQ 240
           KYEPHFDFFHDKVNQELGGHRIATVLMYLSNVEKGGETIFPNS F ESQEKDDS+S CA+
Sbjct: 181 KYEPHFDFFHDKVNQELGGHRIATVLMYLSNVEKGGETIFPNSAF-ESQEKDDSWSDCAR 240

Query: 241 KGYAVKAKKGDALLFFSLHLDASTDTKSLHGSCPVIEGEKWSATKWIHVRSFEKPTRASS 300
           KGYAVKA+KGDALLFFSLHLDA+TD +SLHGSCPVIEGEKWSATKWIHVRSF+K TR SS
Sbjct: 241 KGYAVKAQKGDALLFFSLHLDATTDKRSLHGSCPVIEGEKWSATKWIHVRSFDKATRISS 300

Query: 301 ERCVDENENCPAWAKRGECKKNPTYMVGSEGALGYCRKSCKAC 344
           + CVDEN+NCP+WAKRGEC+KNPTYMVGSEGA+GYCRKSCKAC
Sbjct: 301 QDCVDENKNCPSWAKRGECQKNPTYMVGSEGAVGYCRKSCKAC 309

[Close]

BLAST of Lag0030038 vs. ExPASy TrEMBL
Match: A0A6J1JWX0 (Procollagen-proline 4-dioxygenase OS=Cucurbita maxima OX=3661 GN=LOC111489579 PE=3 SV=1)

HSP 1 Score: 531.2 bits (1367), Expect = 3.3e-147
Identity = 269/343 (78.43%), Postives = 287/343 (83.67%), Query Frame = 0


Query: 1   MDSRRFLALSLCSLFLSAAFARLPETRTHKKLYDPFPLLLALLFRFANPLTRDFGFNCFS 60
           MDSRRFL  SL  L +S  FARLPE  THKKL                            
Sbjct: 1   MDSRRFLGFSLFFLSVSTGFARLPE--THKKL---------------------------- 60

Query: 61  NFRSGSVIRMKGDPAPLIFDPTRVTQLSWRPRAFLYKGFLSDKECDHLIDLAKDKLEKSM 120
              SGSV+ +K D   LIFDPTRVTQLSW+PRAFLYKGFL+D+ECDHLIDLAKDKLEKSM
Sbjct: 61  ---SGSVLELKRDSPRLIFDPTRVTQLSWQPRAFLYKGFLTDQECDHLIDLAKDKLEKSM 120

Query: 121 VADNESGKSVSSEVRTSSGMFLHMAQDEIVAAIEARIAAWTFLPAENGESIQILHYENGQ 180
           VADNESGKSVSSEVRTSSGMFL  AQDEIVA IEARI+AWTFLP ENGESIQILHYENGQ
Sbjct: 121 VADNESGKSVSSEVRTSSGMFLRKAQDEIVAGIEARISAWTFLPVENGESIQILHYENGQ 180

Query: 181 KYEPHFDFFHDKVNQELGGHRIATVLMYLSNVEKGGETIFPNSEFKESQEKDDSFSACAQ 240
           KYEPHFDFFHDKVNQELGGHRIATVLMYLSNVEKGGETIFPNS F ESQEKDDS+S CA+
Sbjct: 181 KYEPHFDFFHDKVNQELGGHRIATVLMYLSNVEKGGETIFPNSAF-ESQEKDDSWSDCAR 240

Query: 241 KGYAVKAKKGDALLFFSLHLDASTDTKSLHGSCPVIEGEKWSATKWIHVRSFEKPTRASS 300
           KGYAVKA+KGDALLFFSLHLDA+TD +SLHGSCPVIEGEKWSATKWIHVRSF+K TR SS
Sbjct: 241 KGYAVKAQKGDALLFFSLHLDATTDKRSLHGSCPVIEGEKWSATKWIHVRSFDKATRTSS 300

Query: 301 ERCVDENENCPAWAKRGECKKNPTYMVGSEGALGYCRKSCKAC 344
           + CVDEN+NCP+WAKRGEC+KNPTYMVGSEGA+GYCRKSCKAC
Sbjct: 301 QDCVDENKNCPSWAKRGECQKNPTYMVGSEGAVGYCRKSCKAC 309

[Close]

BLAST of Lag0030038 vs. TAIR 10
Match: AT3G28480.1 (Oxoglutarate/iron-dependent oxygenase )

HSP 1 Score: 440.7 bits (1132), Expect = 1.1e-123
Identity = 225/343 (65.60%), Postives = 261/343 (76.09%), Query Frame = 0


Query: 1   MDSRRFLALSLCSLFLSAAFARLPETRTHKKLYDPFPLLLALLFRFANPLTRDFGFNCFS 60
           MDSR FLA SLC LF                     PL+ +   RF   LTR       S
Sbjct: 1   MDSRIFLAFSLCFLF-------------------TLPLISSAPNRF---LTRS------S 60

Query: 61  NFRSGSVIRMKGDPAPLIFDPTRVTQLSWRPRAFLYKGFLSDKECDHLIDLAKDKLEKSM 120
           N R GSVI+MK   +   FDPTRVTQLSW PR FLY+GFLSD+ECDH I LAK KLEKSM
Sbjct: 61  NTRDGSVIKMKTSASSFGFDPTRVTQLSWTPRVFLYEGFLSDEECDHFIKLAKGKLEKSM 120

Query: 121 VADNESGKSVSSEVRTSSGMFLHMAQDEIVAAIEARIAAWTFLPAENGESIQILHYENGQ 180
           VADN+SG+SV SEVRTSSGMFL   QD+IV+ +EA++AAWTFLP ENGES+QILHYENGQ
Sbjct: 121 VADNDSGESVESEVRTSSGMFLSKRQDDIVSNVEAKLAAWTFLPEENGESMQILHYENGQ 180

Query: 181 KYEPHFDFFHDKVNQELGGHRIATVLMYLSNVEKGGETIFPNSEFKESQEKDDSFSACAQ 240
           KYEPHFD+FHD+ N ELGGHRIATVLMYLSNVEKGGET+FP  + K +Q KDDS++ CA+
Sbjct: 181 KYEPHFDYFHDQANLELGGHRIATVLMYLSNVEKGGETVFPMWKGKATQLKDDSWTECAK 240

Query: 241 KGYAVKAKKGDALLFFSLHLDASTDTKSLHGSCPVIEGEKWSATKWIHVRSFEKPTRASS 300
           +GYAVK +KGDALLFF+LH +A+TD+ SLHGSCPV+EGEKWSAT+WIHV+SFE+     S
Sbjct: 241 QGYAVKPRKGDALLFFNLHPNATTDSNSLHGSCPVVEGEKWSATRWIHVKSFERAFNKQS 300

Query: 301 ERCVDENENCPAWAKRGECKKNPTYMVGSEGALGYCRKSCKAC 344
             C+DEN +C  WAK GEC+KNPTYMVGS+   GYCRKSCKAC
Sbjct: 301 -GCMDENVSCEKWAKAGECQKNPTYMVGSDKDHGYCRKSCKAC 314

[Close]

BLAST of Lag0030038 vs. TAIR 10
Match: AT3G28480.2 (Oxoglutarate/iron-dependent oxygenase )

HSP 1 Score: 419.1 bits (1076), Expect = 3.5e-117
Identity = 220/351 (62.68%), Postives = 257/351 (73.22%), Query Frame = 0


Query: 1   MDSRRFLALSLCSLFLSAAFARLPETRTHKKLYDPFPLLLALLFRFANPLTRDFGFNCFS 60
           MDSR FLA SLC LF                     PL+ +   RF   LTR       S
Sbjct: 1   MDSRIFLAFSLCFLF-------------------TLPLISSAPNRF---LTRS------S 60

Query: 61  NFRSGSVIRMKGDPAPLIFDPTRVTQLSWRPRAFLYKGFLSDKECDHLIDLAKDKLEKSM 120
           N R GSVI+MK   +   FDPTRVTQLSW PR FLY+GFLSD+ECDH I LAK KLEKSM
Sbjct: 61  NTRDGSVIKMKTSASSFGFDPTRVTQLSWTPRVFLYEGFLSDEECDHFIKLAKGKLEKSM 120

Query: 121 VADNESGKSVSSE-----VRTSSGMFLHMAQ---DEIVAAIEARIAAWTFLPAENGESIQ 180
           VADN+SG+SV SE     VR SS    +M     D+IV+ +EA++AAWTFLP ENGES+Q
Sbjct: 121 VADNDSGESVESEDSVSVVRQSSSFIANMDSLEIDDIVSNVEAKLAAWTFLPEENGESMQ 180

Query: 181 ILHYENGQKYEPHFDFFHDKVNQELGGHRIATVLMYLSNVEKGGETIFPNSEFKESQEKD 240
           ILHYENGQKYEPHFD+FHD+ N ELGGHRIATVLMYLSNVEKGGET+FP  + K +Q KD
Sbjct: 181 ILHYENGQKYEPHFDYFHDQANLELGGHRIATVLMYLSNVEKGGETVFPMWKGKATQLKD 240

Query: 241 DSFSACAQKGYAVKAKKGDALLFFSLHLDASTDTKSLHGSCPVIEGEKWSATKWIHVRSF 300
           DS++ CA++GYAVK +KGDALLFF+LH +A+TD+ SLHGSCPV+EGEKWSAT+WIHV+SF
Sbjct: 241 DSWTECAKQGYAVKPRKGDALLFFNLHPNATTDSNSLHGSCPVVEGEKWSATRWIHVKSF 300

Query: 301 EKPTRASSERCVDENENCPAWAKRGECKKNPTYMVGSEGALGYCRKSCKAC 344
           E+     S  C+DEN +C  WAK GEC+KNPTYMVGS+   GYCRKSCKAC
Sbjct: 301 ERAFNKQS-GCMDENVSCEKWAKAGECQKNPTYMVGSDKDHGYCRKSCKAC 322

[Close]

BLAST of Lag0030038 vs. TAIR 10
Match: AT3G28490.1 (Oxoglutarate/iron-dependent oxygenase )

HSP 1 Score: 403.3 bits (1035), Expect = 2.0e-112
Identity = 195/265 (73.58%), Postives = 224/265 (84.53%), Query Frame = 0


Query: 80  DPTRVTQLSWRPRAFLYKGFLSDKECDHLIDLAKDKLEKSM-VADNESGKSVSSEVRTSS 139
           DPTR+TQLSW PRAFLYKGFLSD+ECDHLI LAK KLEKSM VAD +SG+S  SEVRTSS
Sbjct: 28  DPTRITQLSWTPRAFLYKGFLSDEECDHLIKLAKGKLEKSMVVADVDSGESEDSEVRTSS 87

Query: 140 GMFLHMAQDEIVAAIEARIAAWTFLPAENGESIQILHYENGQKYEPHFDFFHDKVNQELG 199
           GMFL   QD+IVA +EA++AAWTFLP ENGE++QILHYENGQKY+PHFD+F+DK   ELG
Sbjct: 88  GMFLTKRQDDIVANVEAKLAAWTFLPEENGEALQILHYENGQKYDPHFDYFYDKKALELG 147

Query: 200 GHRIATVLMYLSNVEKGGETIFPNSEFKESQEKDDSFSACAQKGYAVKAKKGDALLFFSL 259
           GHRIATVLMYLSNV KGGET+FPN + K  Q KDDS+S CA++GYAVK +KGDALLFF+L
Sbjct: 148 GHRIATVLMYLSNVTKGGETVFPNWKGKTPQLKDDSWSKCAKQGYAVKPRKGDALLFFNL 207

Query: 260 HLDASTDTKSLHGSCPVIEGEKWSATKWIHVRSFEKPTRASSERCVDENENCPAWAKRGE 319
           HL+ +TD  SLHGSCPVIEGEKWSAT+WIHVRSF K        CVD++E+C  WA  GE
Sbjct: 208 HLNGTTDPNSLHGSCPVIEGEKWSATRWIHVRSFGKKKLV----CVDDHESCQEWADAGE 267

Query: 320 CKKNPTYMVGSEGALGYCRKSCKAC 344
           C+KNP YMVGSE +LG+CRKSCKAC
Sbjct: 268 CEKNPMYMVGSETSLGFCRKSCKAC 288

[Close]

BLAST of Lag0030038 vs. TAIR 10
Match: AT5G18900.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 339.3 bits (869), Expect = 3.5e-93
Identity = 162/267 (60.67%), Postives = 202/267 (75.66%), Query Frame = 0


Query: 80  DPTRVTQLSWRPRAFLYKGFLSDKECDHLIDLAKDKLEKSMVADNESGKSVSSEVRTSSG 139
           +P++V Q+S +PRAF+Y+GFL++ ECDH++ LAK  L++S VADN+SG+S  SEVRTSSG
Sbjct: 33  NPSKVKQVSSKPRAFVYEGFLTELECDHMVSLAKASLKRSAVADNDSGESKFSEVRTSSG 92

Query: 140 MFLHMAQDEIVAAIEARIAAWTFLPAENGESIQILHYENGQKYEPHFDFFHDKVNQELGG 199
            F+   +D IV+ IE +I+ WTFLP ENGE IQ+L YE+GQKY+ HFD+FHDKVN   GG
Sbjct: 93  TFISKGKDPIVSGIEDKISTWTFLPKENGEDIQVLRYEHGQKYDAHFDYFHDKVNIVRGG 152

Query: 200 HRIATVLMYLSNVEKGGETIFPNSEFKESQ---EKDDSFSACAQKGYAVKAKKGDALLFF 259
           HR+AT+LMYLSNV KGGET+FP++E    +   E  +  S CA++G AVK +KGDALLFF
Sbjct: 153 HRMATILMYLSNVTKGGETVFPDAEIPSRRVLSENKEDLSDCAKRGIAVKPRKGDALLFF 212

Query: 260 SLHLDASTDTKSLHGSCPVIEGEKWSATKWIHVRSFEKPTRASSERCVDENENCPAWAKR 319
           +LH DA  D  SLHG CPVIEGEKWSATKWIHV SF++    S   C D NE+C  WA  
Sbjct: 213 NLHPDAIPDPLSLHGGCPVIEGEKWSATKWIHVDSFDRIVTPSG-NCTDMNESCERWAVL 272

Query: 320 GECKKNPTYMVGSEGALGYCRKSCKAC 344
           GEC KNP YMVG+    GYCR+SCKAC
Sbjct: 273 GECTKNPEYMVGTTELPGYCRRSCKAC 298

[Close]

BLAST of Lag0030038 vs. TAIR 10
Match: AT3G06300.1 (P4H isoform 2 )

HSP 1 Score: 335.1 bits (858), Expect = 6.6e-92
Identity = 164/269 (60.97%), Postives = 202/269 (75.09%), Query Frame = 0


Query: 78  IFDPTRVTQLSWRPRAFLYKGFLSDKECDHLIDLAKDKLEKSMVADNESGKSVSSEVRTS 137
           I +P++V Q+S +PRAF+Y+GFL+D ECDHLI LAK+ L++S VADN++G+S  S+VRTS
Sbjct: 32  IINPSKVKQVSSKPRAFVYEGFLTDLECDHLISLAKENLQRSAVADNDNGESQVSDVRTS 91

Query: 138 SGMFLHMAQDEIVAAIEARIAAWTFLPAENGESIQILHYENGQKYEPHFDFFHDKVNQEL 197
           SG F+   +D IV+ IE +++ WTFLP ENGE +Q+L YE+GQKY+ HFD+FHDKVN   
Sbjct: 92  SGTFISKGKDPIVSGIEDKLSTWTFLPKENGEDLQVLRYEHGQKYDAHFDYFHDKVNIAR 151

Query: 198 GGHRIATVLMYLSNVEKGGETIFPNS-EF--KESQEKDDSFSACAQKGYAVKAKKGDALL 257
           GGHRIATVL+YLSNV KGGET+FP++ EF  +   E  D  S CA+KG AVK KKG+ALL
Sbjct: 152 GGHRIATVLLYLSNVTKGGETVFPDAQEFSRRSLSENKDDLSDCAKKGIAVKPKKGNALL 211

Query: 258 FFSLHLDASTDTKSLHGSCPVIEGEKWSATKWIHVRSFEKPTRASSERCVDENENCPAWA 317
           FF+L  DA  D  SLHG CPVIEGEKWSATKWIHV SF+K        C D NE+C  WA
Sbjct: 212 FFNLQQDAIPDPFSLHGGCPVIEGEKWSATKWIHVDSFDK-ILTHDGNCTDVNESCERWA 271

Query: 318 KRGECKKNPTYMVGSEGALGYCRKSCKAC 344
             GEC KNP YMVG+    G CR+SCKAC
Sbjct: 272 VLGECGKNPEYMVGTPEIPGNCRRSCKAC 299

The following BLAST results are available for this feature:

BLAST of Lag0030038 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022134044.1	3.0e-155	80.52	probable prolyl 4-hydroxylase 7 [Momordica charantia]	[more]
XP_008458700.1	3.1e-152	79.88	PREDICTED: probable prolyl 4-hydroxylase 7 [Cucumis melo]	[more]
XP_038889686.1	2.9e-150	79.30	probable prolyl 4-hydroxylase 7 isoform X1 [Benincasa hispida]	[more]
XP_038889687.1	3.8e-150	79.30	probable prolyl 4-hydroxylase 7 isoform X2 [Benincasa hispida]	[more]
XP_011655982.1	4.6e-148	78.20	probable prolyl 4-hydroxylase 7 isoform X1 [Cucumis sativus]	[more]

BLAST of Lag0030038 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8L970	1.6e-122	65.60	Probable prolyl 4-hydroxylase 7 OS=Arabidopsis thaliana OX=3702 GN=P4H7 PE=2 SV=...	[more]
F4J0A8	2.8e-111	73.58	Probable prolyl 4-hydroxylase 6 OS=Arabidopsis thaliana OX=3702 GN=P4H6 PE=2 SV=...	[more]
Q8LAN3	4.9e-92	60.67	Probable prolyl 4-hydroxylase 4 OS=Arabidopsis thaliana OX=3702 GN=P4H4 PE=2 SV=...	[more]
F4JAU3	9.3e-91	60.97	Prolyl 4-hydroxylase 2 OS=Arabidopsis thaliana OX=3702 GN=P4H2 PE=1 SV=1	[more]
Q9LN20	4.8e-63	55.77	Probable prolyl 4-hydroxylase 3 OS=Arabidopsis thaliana OX=3702 GN=P4H3 PE=2 SV=...	[more]

BLAST of Lag0030038 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1BXN9	1.5e-155	80.52	Procollagen-proline 4-dioxygenase OS=Momordica charantia OX=3673 GN=LOC111006412...	[more]
A0A1S3C8G4	1.5e-152	79.88	Procollagen-proline 4-dioxygenase OS=Cucumis melo OX=3656 GN=LOC103498028 PE=3 S...	[more]
A0A0A0KS38	2.3e-148	78.20	Procollagen-proline 4-dioxygenase OS=Cucumis sativus OX=3659 GN=Csa_5G633280 PE=...	[more]
A0A6J1FJ93	1.5e-147	78.72	Procollagen-proline 4-dioxygenase OS=Cucurbita moschata OX=3662 GN=LOC111444767 ...	[more]
A0A6J1JWX0	3.3e-147	78.43	Procollagen-proline 4-dioxygenase OS=Cucurbita maxima OX=3661 GN=LOC111489579 PE...	[more]

BLAST of Lag0030038 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G28480.1	1.1e-123	65.60	Oxoglutarate/iron-dependent oxygenase	[more]
AT3G28480.2	3.5e-117	62.68	Oxoglutarate/iron-dependent oxygenase	[more]
AT3G28490.1	2.0e-112	73.58	Oxoglutarate/iron-dependent oxygenase	[more]
AT5G18900.1	3.5e-93	60.67	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT3G06300.1	6.6e-92	60.97	P4H isoform 2	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003582	ShKT domain	SMART	SM00254	ShkT_1	coord: 302..343 e-value: 1.0E-4 score: 31.7
IPR003582	ShKT domain	PFAM	PF01549	ShK	coord: 303..343 e-value: 0.0011 score: 19.5
IPR003582	ShKT domain	PROSITE	PS51670	SHKT	coord: 303..343 score: 9.605646
IPR006620	Prolyl 4-hydroxylase, alpha subunit	SMART	SM00702	p4hc	coord: 91..288 e-value: 3.5E-54 score: 196.0
IPR044862	Prolyl 4-hydroxylase alpha subunit, Fe(2+) 2OG dioxygenase domain	PFAM	PF13640	2OG-FeII_Oxy_3	coord: 172..288 e-value: 4.0E-20 score: 72.5
None	No IPR available	GENE3D	2.60.120.620	q2cbj1_9rhob like domain	coord: 83..289 e-value: 4.6E-76 score: 257.1
None	No IPR available	PANTHER	PTHR10869:SF140	OS03G0803500 PROTEIN	coord: 75..343
IPR045054	Prolyl 4-hydroxylase	PANTHER	PTHR10869	PROLYL 4-HYDROXYLASE ALPHA SUBUNIT	coord: 75..343
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROSITE	PS51471	FE2OG_OXY	coord: 167..289 score: 12.703576

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0030038.1	Lag0030038.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0018401	peptidyl-proline hydroxylation to 4-hydroxy-L-proline
cellular_component	GO:0005789	endoplasmic reticulum membrane
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0031418	L-ascorbic acid binding
molecular_function	GO:0004656	procollagen-proline 4-dioxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen