Lag0029986 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0029986
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionCASP-like protein
Locationchr8: 43618930 .. 43621477 (-)
RNA-Seq ExpressionLag0029986
SyntenyLag0029986
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGCGCATCAAACTCTGAACCAGAAATGAAAAAGGAAGAACCTTCACAATCACAATCCATGGAAGAGCATCAACAACAGCACCATAACCAATCAGAAGAGAACGAAAACGAAAACGAAAATGAAAACGACGACGAATCGCATCCCCCTCTCTCTGCTCACTCCTCACCTCCCCATTCACTCTCCAGCAGCAAAGATCCCTCGCCGCCGGCCCACTCTCCGTCCAACTCCTCCGATTTCTCCGACCACACCTGTCACTCTCATGGCCACTCCTCCATTGACGACGCTCTTTCCATTACGCCGCTCGAGGACTTACCACCGGCGGCTAATCCTCCGTCTCCAAGACCTGTGGCGGCGAACCGCGCGCAGCCGACGGAACCGATTGTGGTGACGAAGGTCGACGCTGAAATACAGGGAGTACGGAAAGTGGAAGAGGGAAGTGATGGCGATGGCGATGACGATGTAGAGAGTGGCGATGGCGGTGCGGTTGGGAGAGGACGGAAGCTGATGGCGAATTTGTCGATGAAGAAACTGAAGAGGGAGGAGTTGAGGAAGAAGATCTTGTTAGGGTTTAGATTTTGTGGATTTGCTTTCTGTTTGGTTTCCTTCTCGGTTATGGCTTCCGACAAGAATCAGGGCTGGGCTTTGGATTCCTTCTATCGCTACAAGGAATTCAGGTTCGTTGAATGAAATCACTTCTTCCTACTGAAATTTCGATTCGTTCTATTGCTCATAATCTCTCTGTAACTGTTAGTATTCAATCGAATTGGGATTTTCATCGAATTTGCAGGTACTGTATGGCAGTGAACGTTATTGGATTTGTTTACTCTGCACTTCAATCATATGATCTTGTATATTTTCTCGCCTCTGGGAAGCACATGATCCGTAATCACTTCAAGCAATACTTCGATTTCTTCATTGATCAGGCAAGTTTCTGTTTGCCTTCGAACTGGGTTCTTAATGTTCAATCCATTAATTATTAATTCTGTGATAATATCAAATACCTAACACTGAGCACATATTGGACGGCCAAGATTAAGCCAATAGTTTAGTCCTGATGACATTATTGTTCATCGATTATGATAGCTTATGGGGACCACAGAAGATAAAAAGATGATGAATAATGCTCAATGGATAGATGGTTCTCTTACTGTATTCCTTTACTGAAATTTTTGTCATTCATATTGAAGCCTTTTCTAACCTTTTCTTATTAAAGAATCTGCAAATTCTTCTCATTATTAAGAGTTTACTGATACTACTAGCTTAAATCCTCTCAATTCATATATAAATAACTTAGAACTATAAAGAGCAGTTTACAGTAGTGGAAGGAAAAACTTAGATTGGTTGAGTAATTTTCATTTATTCTTTCGCTTTAATTTGGCTTAGCTTTTGGGGCATCCTTTTTGTTAACTTTTCTCTGACTTACCTTTATGATAATGCTACCTTCCCCTCGCATCTTTCTTATTTTTCCTTTTTGTGTGTTCAAGTAAGGAAAGTGGCTTTATTTTGAAGCAAAGAATCTAGTATTGAAAATATCACTTGGTTGTGAAAAAATAACTTCATGGTTTATATAACTAGCATAAGCTACTTAAAAGTGCTTTAATGGCAACCACAAACCTTATGTTCTAAAATTCTCAAAAAAAAAGAAAAGATAAAACTTTATATTCTTAGGATGGAGAGCAGAGAGGTGTAACTTCCAAATTGCAACTGGGGAAGTTCGATTTTCTATATCTAAAAAGCCATTTGAATACGACTGTTTACACTTGGATGGTAATATACTTTACCTTATTGCTCCATATGGAAGTGTTGGGCCACTGCTCCGATCCCTATAAAAGAAGGTTCCTCAAGTACCTGAGCTTCATTTTGCACTTTATCTTTAATTATGGGAAAGCTGCAAAAACTGTTGCATTCACACTTCAAACAATTAATTTGAGGAATTACCCTTAAAGTTACAAAATCACTATCGTTATAATTTTGTTAGTAGTTGAGTTAAATTTCAAAGTTTGAGAGTATAATTGCAAAAAACCCTCATTGATCAAGAACAGTTTAAGGTTCGAGATTTAAGTCAGTTGTAAAGACAGCACATTTGTCAAGTTGCAGCTAATGGATTTAGAATGACCGAGTTTGATTGCAATGGTTTCTCAAGTTCAAGAAAGTTAGAGGATGCGTTTTCTAATAACTCCCATAGTTCGAAGTAGTTTTTCCATTTTTCTCAAATAATTTAACAGGAAAAACCTGAACTTTATGGATGCGAACTGAATCATGACACAAACTTTCAACTGCAATTCATTAGTATTTGAGCTTTGCAACATTTGCTGATGAGCTCGAAGAAATTGATGATAGTCTTCTTCTGTTGCAGATAATAGCATATCTTCTTCTATCAGCTTCCTCATCGGCTGCCACAAGAGTTGATGATTGGCAATCCAATTGGGGCAAAGACAAGTTTCCAGATATGGCTACTACGTCCGTAGGATTGTCCATTGTTGCATTTGTAGCTTTTGCCTCGAGTTGTATCATCTCTGGTTATACCCTCTGTAAATCTGCTTAG

mRNA sequence

ATGGGCGCATCAAACTCTGAACCAGAAATGAAAAAGGAAGAACCTTCACAATCACAATCCATGGAAGAGCATCAACAACAGCACCATAACCAATCAGAAGAGAACGAAAACGAAAACGAAAATGAAAACGACGACGAATCGCATCCCCCTCTCTCTGCTCACTCCTCACCTCCCCATTCACTCTCCAGCAGCAAAGATCCCTCGCCGCCGGCCCACTCTCCGTCCAACTCCTCCGATTTCTCCGACCACACCTGTCACTCTCATGGCCACTCCTCCATTGACGACGCTCTTTCCATTACGCCGCTCGAGGACTTACCACCGGCGGCTAATCCTCCGTCTCCAAGACCTGTGGCGGCGAACCGCGCGCAGCCGACGGAACCGATTGTGGTGACGAAGGTCGACGCTGAAATACAGGGAGTACGGAAAGTGGAAGAGGGAAGTGATGGCGATGGCGATGACGATGTAGAGAGTGGCGATGGCGGTGCGGTTGGGAGAGGACGGAAGCTGATGGCGAATTTGTCGATGAAGAAACTGAAGAGGGAGGAGTTGAGGAAGAAGATCTTGTTAGGGTTTAGATTTTGTGGATTTGCTTTCTGTTTGGTTTCCTTCTCGGTTATGGCTTCCGACAAGAATCAGGGCTGGGCTTTGGATTCCTTCTATCGCTACAAGGAATTCAGGTACTGTATGGCAGTGAACGTTATTGGATTTGTTTACTCTGCACTTCAATCATATGATCTTGTATATTTTCTCGCCTCTGGGAAGCACATGATCCGTAATCACTTCAAGCAATACTTCGATTTCTTCATTGATCAGATAATAGCATATCTTCTTCTATCAGCTTCCTCATCGGCTGCCACAAGAGTTGATGATTGGCAATCCAATTGGGGCAAAGACAAGTTTCCAGATATGGCTACTACGTCCGTAGGATTGTCCATTGTTGCATTTGTAGCTTTTGCCTCGAGTTGTATCATCTCTGGTTATACCCTCTGTAAATCTGCTTAG

Coding sequence (CDS)

ATGGGCGCATCAAACTCTGAACCAGAAATGAAAAAGGAAGAACCTTCACAATCACAATCCATGGAAGAGCATCAACAACAGCACCATAACCAATCAGAAGAGAACGAAAACGAAAACGAAAATGAAAACGACGACGAATCGCATCCCCCTCTCTCTGCTCACTCCTCACCTCCCCATTCACTCTCCAGCAGCAAAGATCCCTCGCCGCCGGCCCACTCTCCGTCCAACTCCTCCGATTTCTCCGACCACACCTGTCACTCTCATGGCCACTCCTCCATTGACGACGCTCTTTCCATTACGCCGCTCGAGGACTTACCACCGGCGGCTAATCCTCCGTCTCCAAGACCTGTGGCGGCGAACCGCGCGCAGCCGACGGAACCGATTGTGGTGACGAAGGTCGACGCTGAAATACAGGGAGTACGGAAAGTGGAAGAGGGAAGTGATGGCGATGGCGATGACGATGTAGAGAGTGGCGATGGCGGTGCGGTTGGGAGAGGACGGAAGCTGATGGCGAATTTGTCGATGAAGAAACTGAAGAGGGAGGAGTTGAGGAAGAAGATCTTGTTAGGGTTTAGATTTTGTGGATTTGCTTTCTGTTTGGTTTCCTTCTCGGTTATGGCTTCCGACAAGAATCAGGGCTGGGCTTTGGATTCCTTCTATCGCTACAAGGAATTCAGGTACTGTATGGCAGTGAACGTTATTGGATTTGTTTACTCTGCACTTCAATCATATGATCTTGTATATTTTCTCGCCTCTGGGAAGCACATGATCCGTAATCACTTCAAGCAATACTTCGATTTCTTCATTGATCAGATAATAGCATATCTTCTTCTATCAGCTTCCTCATCGGCTGCCACAAGAGTTGATGATTGGCAATCCAATTGGGGCAAAGACAAGTTTCCAGATATGGCTACTACGTCCGTAGGATTGTCCATTGTTGCATTTGTAGCTTTTGCCTCGAGTTGTATCATCTCTGGTTATACCCTCTGTAAATCTGCTTAG

Protein sequence

MGASNSEPEMKKEEPSQSQSMEEHQQQHHNQSEENENENENENDDESHPPLSAHSSPPHSLSSSKDPSPPAHSPSNSSDFSDHTCHSHGHSSIDDALSITPLEDLPPAANPPSPRPVAANRAQPTEPIVVTKVDAEIQGVRKVEEGSDGDGDDDVESGDGGAVGRGRKLMANLSMKKLKREELRKKILLGFRFCGFAFCLVSFSVMASDKNQGWALDSFYRYKEFRYCMAVNVIGFVYSALQSYDLVYFLASGKHMIRNHFKQYFDFFIDQIIAYLLLSASSSAATRVDDWQSNWGKDKFPDMATTSVGLSIVAFVAFASSCIISGYTLCKSA
Homology
BLAST of Lag0029986 vs. NCBI nr
Match: XP_038890250.1 (CASP-like protein 4A1 [Benincasa hispida])

HSP 1 Score: 533.1 bits (1372), Expect = 1.7e-147
Identity = 292/341 (85.63%), Postives = 307/341 (90.03%), Query Frame = 0

Query: 1   MGASNSEPEMKKEEPSQSQSMEEHQQQ--------HHNQSEENENENENENDDESHPPLS 60
           M AS+SE EMKKEE +++   EE +QQ        HHNQSE      ENENDDESHPPLS
Sbjct: 1   MAASSSEAEMKKEEQAKTMEEEEEEQQQQQQQQKHHHNQSE------ENENDDESHPPLS 60

Query: 61  AHSSPPHSLSSSKDPSPPAHSPSNSSDFSDHTCHSHGHSSIDDALSITPLEDLPPAANPP 120
           A SSPPHSLSSSKDPSPP HSPS SSDFSDHTCH+HG+SSIDDALSIT LEDLPP+ANPP
Sbjct: 61  ARSSPPHSLSSSKDPSPPLHSPSISSDFSDHTCHTHGNSSIDDALSITRLEDLPPSANPP 120

Query: 121 SPRPVAANRAQPTEPIVVTKVDAEIQGVRKVEEGSDGDGDDDVESGDGGAVGRGRKLMAN 180
           SPRPVAANRAQPTEPIVVTKVDAEIQGVRKVEE SD DG  DVESGDGG VGRGRKLMA+
Sbjct: 121 SPRPVAANRAQPTEPIVVTKVDAEIQGVRKVEEVSDSDG--DVESGDGGKVGRGRKLMAS 180

Query: 181 LSMKKLKREELRKKILLGFRFCGFAFCLVSFSVMASDKNQGWALDSFYRYKEFRYCMAVN 240
           LSMKK+KREE RKKILLGFR CGFAFCLVSFSVMASDKNQGWALDSFYRYKEFRYCMAVN
Sbjct: 181 LSMKKMKREESRKKILLGFRICGFAFCLVSFSVMASDKNQGWALDSFYRYKEFRYCMAVN 240

Query: 241 VIGFVYSALQSYDLVYFLASGKHMIRNHFKQYFDFFIDQIIAYLLLSASSSAATRVDDWQ 300
           +IGFVYSALQSYDLVYFL++GKHMIRNHFKQYFDFFIDQIIAYLLLSASSSAATR+DDWQ
Sbjct: 241 IIGFVYSALQSYDLVYFLSTGKHMIRNHFKQYFDFFIDQIIAYLLLSASSSAATRIDDWQ 300

Query: 301 SNWGKDKFPDMATTSVGLSIVAFVAFASSCIISGYTLCKSA 334
           SNWGKDKFPDMAT S+GLSIVAFVAFA SCIISGYTLCKSA
Sbjct: 301 SNWGKDKFPDMATASLGLSIVAFVAFALSCIISGYTLCKSA 333

BLAST of Lag0029986 vs. NCBI nr
Match: XP_023550770.1 (CASP-like protein 4A1 [Cucurbita pepo subsp. pepo] >XP_023550771.1 CASP-like protein 4A1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 521.5 bits (1342), Expect = 5.2e-144
Identity = 287/333 (86.19%), Postives = 301/333 (90.39%), Query Frame = 0

Query: 1   MGASNSEPEMKKEEPSQSQSMEEHQQQHHNQSEENENENENENDDESHPPLSAHSSPPHS 60
           M ASNSE  MKKEE   +++ME+ Q    NQSE NENENENENDDESHPPLSA SSPPHS
Sbjct: 1   MAASNSEAGMKKEE--LAETMEQQQLHRRNQSEVNENENENENDDESHPPLSAQSSPPHS 60

Query: 61  LSSSKDPSPPAHSPSNSSDFSDHTCHSHGHSSIDDALSITPLEDLPPAANPPSPRPVAAN 120
           LS +KDPSPP HSPSNSSDFSDHTC +HG+SSIDDALSIT LEDLPP+A PPSPRPVAAN
Sbjct: 61  LSDNKDPSPPLHSPSNSSDFSDHTCQTHGNSSIDDALSITRLEDLPPSAYPPSPRPVAAN 120

Query: 121 RAQPTEPIVVTKVDAEIQGVRKVEEGSDGDGDDDVESGDGGAVGRGRKLMANLSMKKLKR 180
           RAQPTEPIVVTKVDAEIQGVRKVEEGS  D +     GDGGAVGRGRKLMANLSMKK+KR
Sbjct: 121 RAQPTEPIVVTKVDAEIQGVRKVEEGSASDVEP--SDGDGGAVGRGRKLMANLSMKKIKR 180

Query: 181 EELRKKILLGFRFCGFAFCLVSFSVMASDKNQGWALDSFYRYKEFRYCMAVNVIGFVYSA 240
           EELRKKILLGFR  GF FCL SFSVMASDKNQGWALDSFYRYKEFRYCMAVNVIGFVYSA
Sbjct: 181 EELRKKILLGFRIVGFVFCLASFSVMASDKNQGWALDSFYRYKEFRYCMAVNVIGFVYSA 240

Query: 241 LQSYDLVYFLASGKHMIRNHFKQYFDFFIDQIIAYLLLSASSSAATRVDDWQSNWGKDKF 300
           LQSYDLVYFL++GKHMIRN+FKQYFDFFIDQI+AYLLLSASSSAATRVDDWQSNWGKDKF
Sbjct: 241 LQSYDLVYFLSTGKHMIRNYFKQYFDFFIDQIVAYLLLSASSSAATRVDDWQSNWGKDKF 300

Query: 301 PDMATTSVGLSIVAFVAFASSCIISGYTLCKSA 334
           PDMA TSVGLSIVAFVAFA SCIISGYTLCKSA
Sbjct: 301 PDMAITSVGLSIVAFVAFALSCIISGYTLCKSA 329

BLAST of Lag0029986 vs. NCBI nr
Match: XP_022993636.1 (CASP-like protein 4A1 [Cucurbita maxima] >XP_022993637.1 CASP-like protein 4A1 [Cucurbita maxima])

HSP 1 Score: 511.1 bits (1315), Expect = 7.0e-141
Identity = 281/333 (84.38%), Postives = 299/333 (89.79%), Query Frame = 0

Query: 1   MGASNSEPEMKKEEPSQSQSMEEHQQQHHNQSEENENENENENDDESHPPLSAHSSPPHS 60
           M ASNSE E+KKEE   +++ME+ Q   HNQSE NENENENENDDESHPPLSA SSPPHS
Sbjct: 1   MAASNSEAEVKKEE--LAETMEQQQLHRHNQSEVNENENENENDDESHPPLSAQSSPPHS 60

Query: 61  LSSSKDPSPPAHSPSNSSDFSDHTCHSHGHSSIDDALSITPLEDLPPAANPPSPRPVAAN 120
           LS SKDPSPP HSPSN+SDFSDHTC +HG+SSIDDALSIT LEDL P++ PPSPRPVAAN
Sbjct: 61  LSDSKDPSPPLHSPSNASDFSDHTCQTHGNSSIDDALSITRLEDLSPSSYPPSPRPVAAN 120

Query: 121 RAQPTEPIVVTKVDAEIQGVRKVEEGSDGDGDDDVESGDGGAVGRGRKLMANLSMKKLKR 180
           RAQP+EPIVVTKVDAEIQGVRKVEEGS  D +     GDGG VGRGRKLMANLSMKK+KR
Sbjct: 121 RAQPSEPIVVTKVDAEIQGVRKVEEGSASDVEP--SDGDGGPVGRGRKLMANLSMKKIKR 180

Query: 181 EELRKKILLGFRFCGFAFCLVSFSVMASDKNQGWALDSFYRYKEFRYCMAVNVIGFVYSA 240
           EELRKKILLGFR  GF FCL SFSVMASDKNQGWALDSFYRYKEFRYCMAVNVIGFVYSA
Sbjct: 181 EELRKKILLGFRIVGFVFCLASFSVMASDKNQGWALDSFYRYKEFRYCMAVNVIGFVYSA 240

Query: 241 LQSYDLVYFLASGKHMIRNHFKQYFDFFIDQIIAYLLLSASSSAATRVDDWQSNWGKDKF 300
           LQS+DLVYFL++GK MIRN+FKQYFDFFIDQI+AYLLLSASSSAATRVDDWQSNWG DKF
Sbjct: 241 LQSFDLVYFLSTGKQMIRNYFKQYFDFFIDQIVAYLLLSASSSAATRVDDWQSNWGMDKF 300

Query: 301 PDMATTSVGLSIVAFVAFASSCIISGYTLCKSA 334
           PDMA TSVGLSIVAFVAFA SCIISGYTLCKSA
Sbjct: 301 PDMAITSVGLSIVAFVAFALSCIISGYTLCKSA 329

BLAST of Lag0029986 vs. NCBI nr
Match: XP_022938613.1 (CASP-like protein 4A1 [Cucurbita moschata] >XP_022938614.1 CASP-like protein 4A1 [Cucurbita moschata])

HSP 1 Score: 506.1 bits (1302), Expect = 2.2e-139
Identity = 280/333 (84.08%), Postives = 297/333 (89.19%), Query Frame = 0

Query: 1   MGASNSEPEMKKEEPSQSQSMEEHQQQHHNQSEENENENENENDDESHPPLSAHSSPPHS 60
           M ASNSE E+KKEE   +++ME+ Q   H+QSE   NEN NENDDESHPPLSA SSP HS
Sbjct: 1   MAASNSEAEVKKEE--LAETMEQQQLHRHSQSE--VNENGNENDDESHPPLSAQSSPAHS 60

Query: 61  LSSSKDPSPPAHSPSNSSDFSDHTCHSHGHSSIDDALSITPLEDLPPAANPPSPRPVAAN 120
           LS +KDPSPP HSPSNSSDFSDHTC +HG+SSIDDALSIT LEDLPP+A PPSPRPVAAN
Sbjct: 61  LSDNKDPSPPLHSPSNSSDFSDHTCQTHGNSSIDDALSITRLEDLPPSAYPPSPRPVAAN 120

Query: 121 RAQPTEPIVVTKVDAEIQGVRKVEEGSDGDGDDDVESGDGGAVGRGRKLMANLSMKKLKR 180
           RAQPTEPIVVTKVDAEIQGVRKVEEGS  D +     GDGG VGRGRKLMANLSMKK+KR
Sbjct: 121 RAQPTEPIVVTKVDAEIQGVRKVEEGSASDVEP--SDGDGGPVGRGRKLMANLSMKKIKR 180

Query: 181 EELRKKILLGFRFCGFAFCLVSFSVMASDKNQGWALDSFYRYKEFRYCMAVNVIGFVYSA 240
           EELRKKILLGFR  GF FCL SFSVMASDKNQGWALDSFYRYKEFRYCMAVNVIGFVYSA
Sbjct: 181 EELRKKILLGFRIVGFVFCLASFSVMASDKNQGWALDSFYRYKEFRYCMAVNVIGFVYSA 240

Query: 241 LQSYDLVYFLASGKHMIRNHFKQYFDFFIDQIIAYLLLSASSSAATRVDDWQSNWGKDKF 300
           LQSYDL+YFL++GKHMIRN+FKQYFDFFIDQI+AYLLLSASSSAATRVDDWQSNWG DKF
Sbjct: 241 LQSYDLIYFLSTGKHMIRNYFKQYFDFFIDQIVAYLLLSASSSAATRVDDWQSNWGMDKF 300

Query: 301 PDMATTSVGLSIVAFVAFASSCIISGYTLCKSA 334
           PDMA TSVGLSIVAFVAFA SCIISGYTLCKSA
Sbjct: 301 PDMAVTSVGLSIVAFVAFALSCIISGYTLCKSA 327

BLAST of Lag0029986 vs. NCBI nr
Match: KAG6578643.1 (CASP-like protein 4A1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 503.4 bits (1295), Expect = 1.5e-138
Identity = 278/333 (83.48%), Postives = 295/333 (88.59%), Query Frame = 0

Query: 1   MGASNSEPEMKKEEPSQSQSMEEHQQQHHNQSEENENENENENDDESHPPLSAHSSPPHS 60
           M ASNSE E+KKEE   +++ME+ Q   H+QSE     N+NENDDESHPPLSA SSPPHS
Sbjct: 1   MAASNSEAEVKKEE--LAETMEQQQLHRHSQSE----VNDNENDDESHPPLSAQSSPPHS 60

Query: 61  LSSSKDPSPPAHSPSNSSDFSDHTCHSHGHSSIDDALSITPLEDLPPAANPPSPRPVAAN 120
           LS +KDPSPP HSPSNSSDFSDHTC +HG+SSIDDALSIT LEDLPP+A PPSPRPVAAN
Sbjct: 61  LSDNKDPSPPLHSPSNSSDFSDHTCQTHGNSSIDDALSITRLEDLPPSAYPPSPRPVAAN 120

Query: 121 RAQPTEPIVVTKVDAEIQGVRKVEEGSDGDGDDDVESGDGGAVGRGRKLMANLSMKKLKR 180
           RAQPTEPIVVTKVDAEIQGVRKVEEGS  D +     GDGG VGRGRKLMANLSMKK+KR
Sbjct: 121 RAQPTEPIVVTKVDAEIQGVRKVEEGSASDVEP--SDGDGGPVGRGRKLMANLSMKKIKR 180

Query: 181 EELRKKILLGFRFCGFAFCLVSFSVMASDKNQGWALDSFYRYKEFRYCMAVNVIGFVYSA 240
           EELRKKILLGFR  GF FCL SFSVMASDKNQGWALDSFYRYKEFRYCMAVNVIGFVYSA
Sbjct: 181 EELRKKILLGFRIVGFVFCLASFSVMASDKNQGWALDSFYRYKEFRYCMAVNVIGFVYSA 240

Query: 241 LQSYDLVYFLASGKHMIRNHFKQYFDFFIDQIIAYLLLSASSSAATRVDDWQSNWGKDKF 300
           LQSYDL+YFL++GKHMI N+FKQYFDFFIDQ IAYLLLSASSSAATRVDDWQSNWG DKF
Sbjct: 241 LQSYDLIYFLSTGKHMIHNYFKQYFDFFIDQAIAYLLLSASSSAATRVDDWQSNWGMDKF 300

Query: 301 PDMATTSVGLSIVAFVAFASSCIISGYTLCKSA 334
           PDMA TSVGLSIVAFVAFA SCIISGYTLCKSA
Sbjct: 301 PDMAVTSVGLSIVAFVAFALSCIISGYTLCKSA 325

BLAST of Lag0029986 vs. ExPASy Swiss-Prot
Match: Q84WP5 (CASP-like protein 4A3 OS=Arabidopsis thaliana OX=3702 GN=At2g36330 PE=2 SV=1)

HSP 1 Score: 172.9 bits (437), Expect = 5.9e-42
Identity = 114/295 (38.64%), Postives = 165/295 (55.93%), Query Frame = 0

Query: 49  PPLSAHSSPPHSLSSSKDPSPPAHS------PSNSSDFSDHTC--HSHGHSSIDDALSIT 108
           P  +  S  P S+S+ K P P   S       ++++ FS      HS  HSS  +     
Sbjct: 4   PAKTMPSMSPSSVSTEKSPPPSDTSMAIVAFDNSTTHFSSSPSPPHSLDHSSESEKEDAK 63

Query: 109 PLEDLPPAANP------PSPRPVAANRAQPTEPIVVTKVDAEIQGVRKVEEGSDGDGDDD 168
              +     NP      PSP  V  N  +  + +V T+  A +                 
Sbjct: 64  SKPESRRNKNPGKVEETPSPIVVVHNHNRSVKEVVPTRKSARV----------------- 123

Query: 169 VESGDGGAVGRGRKLMANLSMKKLKREELRKKILLGFRFCGFAFCLVSFSVMASDKNQGW 228
              G G + G+ R    +  +++ +REE+ K   LGFR       L+SFS+MA+DK +GW
Sbjct: 124 ---GSGRSSGQ-RSGAVSAILRRSRREEVVKFSALGFRLSEVVLALISFSIMAADKTKGW 183

Query: 229 ALDSFYRYKEFRYCMAVNVIGFVYSALQSYDLVYFLASGKHMIRNHFKQYFDFFIDQIIA 288
           + DSF RYKE+R+C++VNV+ FVYS+ Q+ DL Y L   KH+I +H +  F+F IDQ++A
Sbjct: 184 SGDSFDRYKEYRFCLSVNVVAFVYSSFQACDLAYHLVKEKHLISHHLRPLFEFIIDQVLA 243

Query: 289 YLLLSASSSAATRVDDWQSNWGKDKFPDMATTSVGLSIVAFVAFASSCIISGYTL 330
           YLL+SAS++A TRVDDW SNWGKD+F +MA+ S+ +S +AF+AFA S +ISGY L
Sbjct: 244 YLLMSASTAAVTRVDDWVSNWGKDEFTEMASASIAMSFLAFLAFAFSSLISGYNL 277

BLAST of Lag0029986 vs. ExPASy Swiss-Prot
Match: D7LIR2 (CASP-like protein 4A3 OS=Arabidopsis lyrata subsp. lyrata OX=81972 GN=ARALYDRAFT_482607 PE=3 SV=1)

HSP 1 Score: 167.2 bits (422), Expect = 3.2e-40
Identity = 109/290 (37.59%), Postives = 159/290 (54.83%), Query Frame = 0

Query: 55  SSPPHSLSSSKDPSPPAHSPSNSSDFSDHTC---------HSHGHSSIDDALSITPLEDL 114
           S  P S+S+ K P PP+ +      F + T          HS  HSS  +        + 
Sbjct: 3   SMSPSSISTEKSP-PPSDTSMAIVAFDNSTTHLSSSPSPPHSLDHSSDSEKEDEKRRPES 62

Query: 115 PPAANP------PSPRPVAANRAQPTEPIVVTKVDAEIQGVRKVEEGSDGDGDDDVESGD 174
               NP      PSP  V  N  +  + +V T+  A +                    G 
Sbjct: 63  RRNKNPVKIEETPSPIVVVHNHNRSVKEVVPTRKTARV--------------------GS 122

Query: 175 GGAVGRGRKLMANLSMKKLKREELRKKILLGFRFCGFAFCLVSFSVMASDKNQGWALDSF 234
           G + G+ R       +++ +REE+ K + LGFR       L+SFS+MA+DK +GW+ DSF
Sbjct: 123 GRSSGQ-RSGAVLAILRRSRREEIVKFVALGFRLSEVVLALISFSIMAADKTKGWSGDSF 182

Query: 235 YRYKEFRYCMAVNVIGFVYSALQSYDLVYFLASGKHMIRNHFKQYFDFFIDQIIAYLLLS 294
            RYKE+R+C++VNV+ F+Y++ Q+ DL Y L   KH+I +H +  F+F IDQ++AYLL+ 
Sbjct: 183 DRYKEYRFCLSVNVVAFIYASFQACDLAYHLVKEKHLISHHLRPLFEFIIDQVLAYLLMC 242

Query: 295 ASSSAATRVDDWQSNWGKDKFPDMATTSVGLSIVAFVAFASSCIISGYTL 330
           AS++A TRVDDW SNWGKD F +MA+ S+ +S + F+AFA S +ISGY L
Sbjct: 243 ASTAAVTRVDDWVSNWGKDDFTEMASASIAMSFLTFLAFAFSSLISGYNL 270

BLAST of Lag0029986 vs. ExPASy Swiss-Prot
Match: Q9FNE8 (CASP-like protein 4A1 OS=Arabidopsis thaliana OX=3702 GN=At5g40300 PE=2 SV=1)

HSP 1 Score: 150.6 bits (379), Expect = 3.1e-35
Identity = 120/310 (38.71%), Postives = 168/310 (54.19%), Query Frame = 0

Query: 21  MEEHQQQHHNQSEENENENENENDDESHPPLSAHSSPPHSLSSSKDPSPPAHSPSNSSDF 80
           MEE ++    Q ++ + + + +     +  +S+ SS  HSL  +   SPP  SP+ SS  
Sbjct: 1   MEELEKTQKFQKKKKQQQEKQDQSSPINFEMSSRSS-LHSLPQTTIESPP-DSPTLSS-- 60

Query: 81  SDHTCHSHGHSSIDDALSITPLEDLPPAANPPSPRPVAANRAQPTEPIVVTKVDAEIQGV 140
                 SHG S               P    P+P     + A+   P  VT         
Sbjct: 61  ---IPDSHGSS---------------PHTIIPTP-----SVAKTETPFRVT--------- 120

Query: 141 RKVEEGSDGDGDDDVESGDGGAVGRGRKLMANLSMKKLKREELRKKILLGFRFCGFAFCL 200
                    +G+++ +  +     R     ++ + ++ K   L +K LLGFR   F  CL
Sbjct: 121 ---------NGEEEKKVSESRRQLRPSFSSSSSTPRESKWASLIRKALLGFRVIAFVSCL 180

Query: 201 VSFSVMASDKNQGWALDSFYRYKEFRYCMAVNVIGFVYSALQSYDLVYFLASGKHMIRNH 260
           VSFSVM SD+++GWA DSFY YKEFR+C+A NVIGFVYS     DLVY L++     R++
Sbjct: 181 VSFSVMVSDRDKGWAHDSFYNYKEFRFCLAANVIGFVYSGFMICDLVYLLSTSIRRSRHN 240

Query: 261 FKQYFDFFIDQIIAYLLLSASSSAATRVDDWQSNWGKDKFPDMATTSVGLSIVAFVAFAS 320
            + + +F +DQ++AYLL SAS+SA+ RVDDWQSNWG DKFPD+A  SV LS V+FVAFA 
Sbjct: 241 LRHFLEFGLDQMLAYLLASASTSASIRVDDWQSNWGADKFPDLARASVALSYVSFVAFAF 265

Query: 321 SCIISGYTLC 331
             + SGY LC
Sbjct: 301 CSLASGYALC 265

BLAST of Lag0029986 vs. ExPASy Swiss-Prot
Match: D7MMW4 (CASP-like protein 4A2 OS=Arabidopsis lyrata subsp. lyrata OX=81972 GN=ARALYDRAFT_919556 PE=3 SV=1)

HSP 1 Score: 147.5 bits (371), Expect = 2.7e-34
Identity = 109/295 (36.95%), Postives = 153/295 (51.86%), Query Frame = 0

Query: 35  NENENENENDDESHPPLSAHSSPPHSLSSSKDPSPPAHSPSNSSDFSDHTCHSHGHSSID 94
           + N      ++  H PL  HS     LS + D     +     S F           SI 
Sbjct: 8   SSNSEAYSYNESPHSPLRFHS----PLSDAGDLPESRYVSPEGSPFK-----IENPKSIV 67

Query: 95  DALSITPLEDLPPAANPPSPRPVAANRAQPTEPIVVTKVDAEIQGVRKVEEGSDGDGDDD 154
               +T    LP    PP P+     R +     + + +D     +         DG  +
Sbjct: 68  AGNKLTQFSPLPSPIPPPPPQIPPPRRQRNARVPMNSSLDKSPSSMVVQNSWVREDGQQN 127

Query: 155 VESGDGGAVGRGRKLMANLSMKKLKREELRKKILLGFRFCGFAFCLVSFSVMASDKNQGW 214
             +   GA   G +  A  ++ + +R++L     LGFR      C++SFS+MA+DK QGW
Sbjct: 128 T-TRKAGAPMNGEE-SATTAVNRARRDDLVSVTALGFRITEVILCVISFSIMAADKTQGW 187

Query: 215 ALDSFYRYKEFRYCMAVNVIGFVYSALQSYDLVYFLASGKHMIRNHFKQYFDFFIDQIIA 274
           + DS+ RYKE+RYC+AVNVI FVYSA ++ D   ++A   +M+   F   F F +DQ++A
Sbjct: 188 SGDSYDRYKEYRYCLAVNVIAFVYSAFEACDAACYMAKESYMMNCGFHDLFVFSMDQLLA 247

Query: 275 YLLLSASSSAATRVDDWQSNWGKDKFPDMATTSVGLSIVAFVAFASSCIISGYTL 330
           YLL+SASS AATRVDDW SNWGKD+F  MAT S+ +S +AF AFA S +IS Y L
Sbjct: 248 YLLMSASSCAATRVDDWVSNWGKDEFTQMATASIAVSFLAFGAFAVSALISSYRL 291

BLAST of Lag0029986 vs. ExPASy Swiss-Prot
Match: Q501G6 (CASP-like protein 4A2 OS=Arabidopsis thaliana OX=3702 GN=At5g62820 PE=2 SV=1)

HSP 1 Score: 142.1 bits (357), Expect = 1.1e-32
Identity = 106/295 (35.93%), Postives = 146/295 (49.49%), Query Frame = 0

Query: 35  NENENENENDDESHPPLSAHSSPPHSLSSSKDPSPPAHSPSNSSDFSDHTCHSHGHSSID 94
           + N      ++  H PL  HS     LS + D     +     S F           SI 
Sbjct: 8   SSNSEAQSYNESPHSPLRFHS----PLSDAGDLPESRYVSPEGSPFK-----IENPKSIV 67

Query: 95  DALSITPLEDLPPAANPPSPRPVAANRAQPTEPIVVTKVDAEIQGVRKVEEGSDGDGDDD 154
               +T    LP    PP P+     R +     + +  D     +         DG   
Sbjct: 68  AGNKLTQFSPLPSPIPPPPPQFPPPRRQRNARVPMNSSSDKSPSSMVVHNSWVREDGGQT 127

Query: 155 VESGDGGAVGRGRKLMANLSMKKLKREELRKKILLGFRFCGFAFCLVSFSVMASDKNQGW 214
                G  +          ++ + + ++L     LGFR      C++SFS+MA+DK QGW
Sbjct: 128 TTRKAGAPINGEES--TRTTVNRARGDDLVSLTALGFRITEVILCVISFSIMAADKTQGW 187

Query: 215 ALDSFYRYKEFRYCMAVNVIGFVYSALQSYDLVYFLASGKHMIRNHFKQYFDFFIDQIIA 274
           + DS+ RYKE+RYC+AVNVI FVYSA ++ D   ++A   +MI   F   F F +DQ++A
Sbjct: 188 SGDSYDRYKEYRYCLAVNVIAFVYSAFEACDAACYIAKESYMINCGFHDLFVFSMDQLLA 247

Query: 275 YLLLSASSSAATRVDDWQSNWGKDKFPDMATTSVGLSIVAFVAFASSCIISGYTL 330
           YLL+SASS AATRVDDW SNWGKD+F  MAT S+ +S +AF AFA S +IS Y L
Sbjct: 248 YLLMSASSCAATRVDDWVSNWGKDEFTQMATASIAVSFLAFGAFAVSALISSYRL 291

BLAST of Lag0029986 vs. ExPASy TrEMBL
Match: A0A6J1JTE4 (CASP-like protein OS=Cucurbita maxima OX=3661 GN=LOC111489567 PE=3 SV=1)

HSP 1 Score: 511.1 bits (1315), Expect = 3.4e-141
Identity = 281/333 (84.38%), Postives = 299/333 (89.79%), Query Frame = 0

Query: 1   MGASNSEPEMKKEEPSQSQSMEEHQQQHHNQSEENENENENENDDESHPPLSAHSSPPHS 60
           M ASNSE E+KKEE   +++ME+ Q   HNQSE NENENENENDDESHPPLSA SSPPHS
Sbjct: 1   MAASNSEAEVKKEE--LAETMEQQQLHRHNQSEVNENENENENDDESHPPLSAQSSPPHS 60

Query: 61  LSSSKDPSPPAHSPSNSSDFSDHTCHSHGHSSIDDALSITPLEDLPPAANPPSPRPVAAN 120
           LS SKDPSPP HSPSN+SDFSDHTC +HG+SSIDDALSIT LEDL P++ PPSPRPVAAN
Sbjct: 61  LSDSKDPSPPLHSPSNASDFSDHTCQTHGNSSIDDALSITRLEDLSPSSYPPSPRPVAAN 120

Query: 121 RAQPTEPIVVTKVDAEIQGVRKVEEGSDGDGDDDVESGDGGAVGRGRKLMANLSMKKLKR 180
           RAQP+EPIVVTKVDAEIQGVRKVEEGS  D +     GDGG VGRGRKLMANLSMKK+KR
Sbjct: 121 RAQPSEPIVVTKVDAEIQGVRKVEEGSASDVEP--SDGDGGPVGRGRKLMANLSMKKIKR 180

Query: 181 EELRKKILLGFRFCGFAFCLVSFSVMASDKNQGWALDSFYRYKEFRYCMAVNVIGFVYSA 240
           EELRKKILLGFR  GF FCL SFSVMASDKNQGWALDSFYRYKEFRYCMAVNVIGFVYSA
Sbjct: 181 EELRKKILLGFRIVGFVFCLASFSVMASDKNQGWALDSFYRYKEFRYCMAVNVIGFVYSA 240

Query: 241 LQSYDLVYFLASGKHMIRNHFKQYFDFFIDQIIAYLLLSASSSAATRVDDWQSNWGKDKF 300
           LQS+DLVYFL++GK MIRN+FKQYFDFFIDQI+AYLLLSASSSAATRVDDWQSNWG DKF
Sbjct: 241 LQSFDLVYFLSTGKQMIRNYFKQYFDFFIDQIVAYLLLSASSSAATRVDDWQSNWGMDKF 300

Query: 301 PDMATTSVGLSIVAFVAFASSCIISGYTLCKSA 334
           PDMA TSVGLSIVAFVAFA SCIISGYTLCKSA
Sbjct: 301 PDMAITSVGLSIVAFVAFALSCIISGYTLCKSA 329

BLAST of Lag0029986 vs. ExPASy TrEMBL
Match: A0A6J1FJD7 (CASP-like protein OS=Cucurbita moschata OX=3662 GN=LOC111444795 PE=3 SV=1)

HSP 1 Score: 506.1 bits (1302), Expect = 1.1e-139
Identity = 280/333 (84.08%), Postives = 297/333 (89.19%), Query Frame = 0

Query: 1   MGASNSEPEMKKEEPSQSQSMEEHQQQHHNQSEENENENENENDDESHPPLSAHSSPPHS 60
           M ASNSE E+KKEE   +++ME+ Q   H+QSE   NEN NENDDESHPPLSA SSP HS
Sbjct: 1   MAASNSEAEVKKEE--LAETMEQQQLHRHSQSE--VNENGNENDDESHPPLSAQSSPAHS 60

Query: 61  LSSSKDPSPPAHSPSNSSDFSDHTCHSHGHSSIDDALSITPLEDLPPAANPPSPRPVAAN 120
           LS +KDPSPP HSPSNSSDFSDHTC +HG+SSIDDALSIT LEDLPP+A PPSPRPVAAN
Sbjct: 61  LSDNKDPSPPLHSPSNSSDFSDHTCQTHGNSSIDDALSITRLEDLPPSAYPPSPRPVAAN 120

Query: 121 RAQPTEPIVVTKVDAEIQGVRKVEEGSDGDGDDDVESGDGGAVGRGRKLMANLSMKKLKR 180
           RAQPTEPIVVTKVDAEIQGVRKVEEGS  D +     GDGG VGRGRKLMANLSMKK+KR
Sbjct: 121 RAQPTEPIVVTKVDAEIQGVRKVEEGSASDVEP--SDGDGGPVGRGRKLMANLSMKKIKR 180

Query: 181 EELRKKILLGFRFCGFAFCLVSFSVMASDKNQGWALDSFYRYKEFRYCMAVNVIGFVYSA 240
           EELRKKILLGFR  GF FCL SFSVMASDKNQGWALDSFYRYKEFRYCMAVNVIGFVYSA
Sbjct: 181 EELRKKILLGFRIVGFVFCLASFSVMASDKNQGWALDSFYRYKEFRYCMAVNVIGFVYSA 240

Query: 241 LQSYDLVYFLASGKHMIRNHFKQYFDFFIDQIIAYLLLSASSSAATRVDDWQSNWGKDKF 300
           LQSYDL+YFL++GKHMIRN+FKQYFDFFIDQI+AYLLLSASSSAATRVDDWQSNWG DKF
Sbjct: 241 LQSYDLIYFLSTGKHMIRNYFKQYFDFFIDQIVAYLLLSASSSAATRVDDWQSNWGMDKF 300

Query: 301 PDMATTSVGLSIVAFVAFASSCIISGYTLCKSA 334
           PDMA TSVGLSIVAFVAFA SCIISGYTLCKSA
Sbjct: 301 PDMAVTSVGLSIVAFVAFALSCIISGYTLCKSA 327

BLAST of Lag0029986 vs. ExPASy TrEMBL
Match: A0A6J1BX26 (CASP-like protein OS=Momordica charantia OX=3673 GN=LOC111006289 PE=3 SV=1)

HSP 1 Score: 483.0 bits (1242), Expect = 9.9e-133
Identity = 267/333 (80.18%), Postives = 288/333 (86.49%), Query Frame = 0

Query: 3   ASNSEPEMKKEEPSQSQSMEEHQQQHHNQSEENENENENENDDESHPPLSAHSSPPHSLS 62
           A++SE EMKKEE                 +E    +   +NDDESHPPLSA SSPPHSLS
Sbjct: 2   AASSEAEMKKEE-----------------AEGTTEQYPEQNDDESHPPLSARSSPPHSLS 61

Query: 63  --SSKDPSPPAHSPSNSSDFSDHTCHSHGHSSIDDALSITPLEDLPPAANPPSPRPVAAN 122
              S+ PSPPAHS S SSDFSDHTCHSHGHSSIDD+LSITP+E+LPP  NPPSPRPVAAN
Sbjct: 62  GPGSQRPSPPAHSLSLSSDFSDHTCHSHGHSSIDDSLSITPMENLPP-CNPPSPRPVAAN 121

Query: 123 RAQPTEPIVVTKVDAEIQGVRKVEEGSDGDGDDDVESGDGGAVGRGRKLMANLSMKKLKR 182
           RAQPTEPIVVT+VDAEIQGVRKV++ SD     DVESGDGGAVGRGRKLMA+LSMKK+KR
Sbjct: 122 RAQPTEPIVVTEVDAEIQGVRKVQDVSDA----DVESGDGGAVGRGRKLMASLSMKKMKR 181

Query: 183 EELRKKILLGFRFCGFAFCLVSFSVMASDKNQGWALDSFYRYKEFRYCMAVNVIGFVYSA 242
           EE+RKKILLGFR CGFAFCLVSFSVMASDKNQGWALDSFYRYKEFRYCMAVNVIGF+YSA
Sbjct: 182 EEMRKKILLGFRICGFAFCLVSFSVMASDKNQGWALDSFYRYKEFRYCMAVNVIGFLYSA 241

Query: 243 LQSYDLVYFLASGKHMIRNHFKQYFDFFIDQIIAYLLLSASSSAATRVDDWQSNWGKDKF 302
           LQSYDLVYFL++GKHMI NHFKQYFDFFIDQI+AYLLLSASSSAATRVDDWQSNWGKDKF
Sbjct: 242 LQSYDLVYFLSTGKHMILNHFKQYFDFFIDQIVAYLLLSASSSAATRVDDWQSNWGKDKF 301

Query: 303 PDMATTSVGLSIVAFVAFASSCIISGYTLCKSA 334
           PDMA  SVGLSIVAFVAFA SC+ISG+TLCKSA
Sbjct: 302 PDMAAASVGLSIVAFVAFALSCLISGHTLCKSA 312

BLAST of Lag0029986 vs. ExPASy TrEMBL
Match: A0A0A0KS01 (CASP-like protein OS=Cucumis sativus OX=3659 GN=Csa_5G630810 PE=3 SV=1)

HSP 1 Score: 482.3 bits (1240), Expect = 1.7e-132
Identity = 270/337 (80.12%), Postives = 291/337 (86.35%), Query Frame = 0

Query: 3   ASNSEPEMKKEE-PSQSQSMEEHQQQHHNQSEENENENENENDDESHPPLSAHSSPPHSL 62
           ASNSE EMKKEE P   +   E +  HHNQS+E E++N      ESH  LSA SSPPHSL
Sbjct: 7   ASNSEAEMKKEELPKSMEEPPEQEHPHHNQSDEGEDDN------ESHLHLSACSSPPHSL 66

Query: 63  SSSKDPSPPAHSPSNSSDFSDHTCHSHGHSSIDDALSITPLED--LPPAANPPSPRPVAA 122
           S++KDPSPP HSPS SSDFSDHTCHS G+SSID ALSITPL+D  LPP+AN PSPRPVAA
Sbjct: 67  STNKDPSPPLHSPSISSDFSDHTCHSPGNSSIDHALSITPLQDLHLPPSANSPSPRPVAA 126

Query: 123 NRAQPTEPIVVTKVDAEIQGVRKVEEGSDGDGD---DDVESGDGGAVGRGRKLMANLSMK 182
           NRAQP+EPIVVTKVD EIQG+RKVEE  D DGD    D + GDGG VGRGRKLM  LS+K
Sbjct: 127 NRAQPSEPIVVTKVDTEIQGIRKVEEVIDSDGDVESGDGDDGDGGKVGRGRKLMETLSIK 186

Query: 183 KLKREELRKKILLGFRFCGFAFCLVSFSVMASDKNQGWALDSFYRYKEFRYCMAVNVIGF 242
           K+KREELRKKILLGFR CGFAFCLVS SVMASDK+QGWALDSFYRYKEFRYCMAVNVIGF
Sbjct: 187 KIKREELRKKILLGFRICGFAFCLVSVSVMASDKDQGWALDSFYRYKEFRYCMAVNVIGF 246

Query: 243 VYSALQSYDLVYFLASGKHMIRNHFKQYFDFFIDQIIAYLLLSASSSAATRVDDWQSNWG 302
           VYSALQSYDLVYFL++GKH+IRNHFKQYFDFFIDQIIAYLLLSASSSAATR+DDWQSNWG
Sbjct: 247 VYSALQSYDLVYFLSTGKHVIRNHFKQYFDFFIDQIIAYLLLSASSSAATRIDDWQSNWG 306

Query: 303 KDKFPDMATTSVGLSIVAFVAFASSCIISGYTLCKSA 334
           KDKFPDMAT S+G SIVAFVAFA SCIISGYTLCKSA
Sbjct: 307 KDKFPDMATGSLGFSIVAFVAFALSCIISGYTLCKSA 337

BLAST of Lag0029986 vs. ExPASy TrEMBL
Match: A0A5A7T7H8 (CASP-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold808G00890 PE=3 SV=1)

HSP 1 Score: 478.8 bits (1231), Expect = 1.9e-131
Identity = 269/331 (81.27%), Postives = 292/331 (88.22%), Query Frame = 0

Query: 3   ASNSEPEMKKEEPSQSQSMEEHQQQHHNQSEENENENENENDDESHPPLSAHSSPPHSLS 62
           ASNSE EMK+EE    +SMEE Q+Q H Q   N+++ E END+ESH  LSA SSPPHSLS
Sbjct: 5   ASNSEAEMKREE--LPKSMEEPQEQVHEQPHHNQSD-EGENDNESHLHLSACSSPPHSLS 64

Query: 63  SSKDPSPPAHSPSNSSDFSDHTCHSHGHSSIDDALSITPLEDLPPAANPPSPRPVAANRA 122
           +SKDPSPP HSPS SSDFSDHTCHS G+SSIDD      ++DLPP+ANPPSPRPVAANRA
Sbjct: 65  TSKDPSPPLHSPSISSDFSDHTCHSRGNSSIDD------VQDLPPSANPPSPRPVAANRA 124

Query: 123 QPTEPIVVTKVDAEIQGVRKVEEGSDGDGDDDVESGDGGAVGRGRKLMANLSMKKLKREE 182
           QP+EPIVVTKVD EIQGVRKVEE SD DG  DVES DGG VGRGRKLM +LS+KK+K EE
Sbjct: 125 QPSEPIVVTKVDTEIQGVRKVEEVSDSDG--DVESADGGKVGRGRKLMESLSIKKIKEEE 184

Query: 183 LRKKILLGFRFCGFAFCLVSFSVMASDKNQGWALDSFYRYKEFRYCMAVNVIGFVYSALQ 242
           LRKKILLGFR CGF+FCLVS SVMASDK+QGWALDSFYRYKEFRYCMAVNVIGFVYSALQ
Sbjct: 185 LRKKILLGFRICGFSFCLVSVSVMASDKDQGWALDSFYRYKEFRYCMAVNVIGFVYSALQ 244

Query: 243 SYDLVYFLASGKHMIRNHFKQYFDFFIDQIIAYLLLSASSSAATRVDDWQSNWGKDKFPD 302
           SYDLVYFL++GKHMIRNHFKQYFDFFIDQIIAYLLLSASSSAATR+DDWQSNWG+DKFPD
Sbjct: 245 SYDLVYFLSTGKHMIRNHFKQYFDFFIDQIIAYLLLSASSSAATRIDDWQSNWGRDKFPD 304

Query: 303 MATTSVGLSIVAFVAFASSCIISGYTLCKSA 334
           MAT S+ LSIVAFVAFA SCIISGYTLCKSA
Sbjct: 305 MATGSLALSIVAFVAFALSCIISGYTLCKSA 324

BLAST of Lag0029986 vs. TAIR 10
Match: AT2G36330.1 (Uncharacterised protein family (UPF0497) )

HSP 1 Score: 172.9 bits (437), Expect = 4.2e-43
Identity = 114/295 (38.64%), Postives = 165/295 (55.93%), Query Frame = 0

Query: 49  PPLSAHSSPPHSLSSSKDPSPPAHS------PSNSSDFSDHTC--HSHGHSSIDDALSIT 108
           P  +  S  P S+S+ K P P   S       ++++ FS      HS  HSS  +     
Sbjct: 4   PAKTMPSMSPSSVSTEKSPPPSDTSMAIVAFDNSTTHFSSSPSPPHSLDHSSESEKEDAK 63

Query: 109 PLEDLPPAANP------PSPRPVAANRAQPTEPIVVTKVDAEIQGVRKVEEGSDGDGDDD 168
              +     NP      PSP  V  N  +  + +V T+  A +                 
Sbjct: 64  SKPESRRNKNPGKVEETPSPIVVVHNHNRSVKEVVPTRKSARV----------------- 123

Query: 169 VESGDGGAVGRGRKLMANLSMKKLKREELRKKILLGFRFCGFAFCLVSFSVMASDKNQGW 228
              G G + G+ R    +  +++ +REE+ K   LGFR       L+SFS+MA+DK +GW
Sbjct: 124 ---GSGRSSGQ-RSGAVSAILRRSRREEVVKFSALGFRLSEVVLALISFSIMAADKTKGW 183

Query: 229 ALDSFYRYKEFRYCMAVNVIGFVYSALQSYDLVYFLASGKHMIRNHFKQYFDFFIDQIIA 288
           + DSF RYKE+R+C++VNV+ FVYS+ Q+ DL Y L   KH+I +H +  F+F IDQ++A
Sbjct: 184 SGDSFDRYKEYRFCLSVNVVAFVYSSFQACDLAYHLVKEKHLISHHLRPLFEFIIDQVLA 243

Query: 289 YLLLSASSSAATRVDDWQSNWGKDKFPDMATTSVGLSIVAFVAFASSCIISGYTL 330
           YLL+SAS++A TRVDDW SNWGKD+F +MA+ S+ +S +AF+AFA S +ISGY L
Sbjct: 244 YLLMSASTAAVTRVDDWVSNWGKDEFTEMASASIAMSFLAFLAFAFSSLISGYNL 277

BLAST of Lag0029986 vs. TAIR 10
Match: AT5G40300.1 (Uncharacterised protein family (UPF0497) )

HSP 1 Score: 150.6 bits (379), Expect = 2.2e-36
Identity = 120/310 (38.71%), Postives = 168/310 (54.19%), Query Frame = 0

Query: 21  MEEHQQQHHNQSEENENENENENDDESHPPLSAHSSPPHSLSSSKDPSPPAHSPSNSSDF 80
           MEE ++    Q ++ + + + +     +  +S+ SS  HSL  +   SPP  SP+ SS  
Sbjct: 1   MEELEKTQKFQKKKKQQQEKQDQSSPINFEMSSRSS-LHSLPQTTIESPP-DSPTLSS-- 60

Query: 81  SDHTCHSHGHSSIDDALSITPLEDLPPAANPPSPRPVAANRAQPTEPIVVTKVDAEIQGV 140
                 SHG S               P    P+P     + A+   P  VT         
Sbjct: 61  ---IPDSHGSS---------------PHTIIPTP-----SVAKTETPFRVT--------- 120

Query: 141 RKVEEGSDGDGDDDVESGDGGAVGRGRKLMANLSMKKLKREELRKKILLGFRFCGFAFCL 200
                    +G+++ +  +     R     ++ + ++ K   L +K LLGFR   F  CL
Sbjct: 121 ---------NGEEEKKVSESRRQLRPSFSSSSSTPRESKWASLIRKALLGFRVIAFVSCL 180

Query: 201 VSFSVMASDKNQGWALDSFYRYKEFRYCMAVNVIGFVYSALQSYDLVYFLASGKHMIRNH 260
           VSFSVM SD+++GWA DSFY YKEFR+C+A NVIGFVYS     DLVY L++     R++
Sbjct: 181 VSFSVMVSDRDKGWAHDSFYNYKEFRFCLAANVIGFVYSGFMICDLVYLLSTSIRRSRHN 240

Query: 261 FKQYFDFFIDQIIAYLLLSASSSAATRVDDWQSNWGKDKFPDMATTSVGLSIVAFVAFAS 320
            + + +F +DQ++AYLL SAS+SA+ RVDDWQSNWG DKFPD+A  SV LS V+FVAFA 
Sbjct: 241 LRHFLEFGLDQMLAYLLASASTSASIRVDDWQSNWGADKFPDLARASVALSYVSFVAFAF 265

Query: 321 SCIISGYTLC 331
             + SGY LC
Sbjct: 301 CSLASGYALC 265

BLAST of Lag0029986 vs. TAIR 10
Match: AT5G62820.1 (Uncharacterised protein family (UPF0497) )

HSP 1 Score: 142.1 bits (357), Expect = 7.9e-34
Identity = 106/295 (35.93%), Postives = 146/295 (49.49%), Query Frame = 0

Query: 35  NENENENENDDESHPPLSAHSSPPHSLSSSKDPSPPAHSPSNSSDFSDHTCHSHGHSSID 94
           + N      ++  H PL  HS     LS + D     +     S F           SI 
Sbjct: 8   SSNSEAQSYNESPHSPLRFHS----PLSDAGDLPESRYVSPEGSPFK-----IENPKSIV 67

Query: 95  DALSITPLEDLPPAANPPSPRPVAANRAQPTEPIVVTKVDAEIQGVRKVEEGSDGDGDDD 154
               +T    LP    PP P+     R +     + +  D     +         DG   
Sbjct: 68  AGNKLTQFSPLPSPIPPPPPQFPPPRRQRNARVPMNSSSDKSPSSMVVHNSWVREDGGQT 127

Query: 155 VESGDGGAVGRGRKLMANLSMKKLKREELRKKILLGFRFCGFAFCLVSFSVMASDKNQGW 214
                G  +          ++ + + ++L     LGFR      C++SFS+MA+DK QGW
Sbjct: 128 TTRKAGAPINGEES--TRTTVNRARGDDLVSLTALGFRITEVILCVISFSIMAADKTQGW 187

Query: 215 ALDSFYRYKEFRYCMAVNVIGFVYSALQSYDLVYFLASGKHMIRNHFKQYFDFFIDQIIA 274
           + DS+ RYKE+RYC+AVNVI FVYSA ++ D   ++A   +MI   F   F F +DQ++A
Sbjct: 188 SGDSYDRYKEYRYCLAVNVIAFVYSAFEACDAACYIAKESYMINCGFHDLFVFSMDQLLA 247

Query: 275 YLLLSASSSAATRVDDWQSNWGKDKFPDMATTSVGLSIVAFVAFASSCIISGYTL 330
           YLL+SASS AATRVDDW SNWGKD+F  MAT S+ +S +AF AFA S +IS Y L
Sbjct: 248 YLLMSASSCAATRVDDWVSNWGKDEFTQMATASIAVSFLAFGAFAVSALISSYRL 291

BLAST of Lag0029986 vs. TAIR 10
Match: AT2G38480.1 (Uncharacterised protein family (UPF0497) )

HSP 1 Score: 74.7 bits (182), Expect = 1.6e-13
Identity = 55/155 (35.48%), Postives = 92/155 (59.35%), Query Frame = 0

Query: 176 KKLKREELRKKILLGFRFCGFAFCLVSFSVMASDKNQGWALDSFYRYKEFRYCMAVNVIG 235
           ++ KRE+L KK     R     F L++F +M S+K+ G+   +F  Y+E+RY +A+++I 
Sbjct: 36  QRWKREDLIKKASPITRGICLLFSLIAFLIMVSNKH-GYG-RNFNDYEEYRYVLAISIIS 95

Query: 236 FVYSALQSYDLVYFLASGKHMIRNHFKQYFDFFIDQIIAYLLLSASSSAATRVDDWQSNW 295
            +Y+A Q++   +F  S + +         DF  DQI+AYLL+SA+SSA    + ++   
Sbjct: 96  TLYTAWQTF--AHF--SKREIFDRRTSILVDFSGDQIVAYLLISAASSAIPLTNIFRE-- 155

Query: 296 GKDK-FPDMATTSVGLSIVAFVAFASSCIISGYTL 330
           G+D  F D A +++ ++I AF+A A S + SGY L
Sbjct: 156 GQDNIFTDSAASAISMAIFAFIALALSALFSGYKL 182

BLAST of Lag0029986 vs. TAIR 10
Match: AT4G11655.1 (Uncharacterised protein family (UPF0497) )

HSP 1 Score: 70.5 bits (171), Expect = 2.9e-12
Identity = 51/152 (33.55%), Postives = 71/152 (46.71%), Query Frame = 0

Query: 189 LGFRFCGFAFCLVSFSVMA------SDKNQGWALDSFYRYKEFRYCMAVNVIGFVYSALQ 248
           L  RF     C VS   +A      S ++      SF  Y E  YC  V VIGFVY++LQ
Sbjct: 57  LVLRFITMVLCFVSALSLAVNVQRPSKRHLTQNSSSFASYPELLYCFGVAVIGFVYTSLQ 116

Query: 249 SYDLVYFLASGKHMIRNHFKQYFDFFIDQIIAYLLLSASSSAATRVDDWQSNWGKDKFPD 308
           ++  V  +     +I      Y  F  DQ+I YLL+S+SS A      W  +  +D    
Sbjct: 117 TFKGVCDITHRGVLISEPLSDYISFIFDQVICYLLVSSSSVAIA----WIQHINEDAIKT 176

Query: 309 M---ATTSVGLSIVAFVAFASSCIISGYTLCK 332
           +   +  SV +S  AF+    S ++SGY LCK
Sbjct: 177 LRNNSIVSVSMSFSAFLVLTLSGLLSGYKLCK 204

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038890250.11.7e-14785.63CASP-like protein 4A1 [Benincasa hispida][more]
XP_023550770.15.2e-14486.19CASP-like protein 4A1 [Cucurbita pepo subsp. pepo] >XP_023550771.1 CASP-like pro... [more]
XP_022993636.17.0e-14184.38CASP-like protein 4A1 [Cucurbita maxima] >XP_022993637.1 CASP-like protein 4A1 [... [more]
XP_022938613.12.2e-13984.08CASP-like protein 4A1 [Cucurbita moschata] >XP_022938614.1 CASP-like protein 4A1... [more]
KAG6578643.11.5e-13883.48CASP-like protein 4A1, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
Q84WP55.9e-4238.64CASP-like protein 4A3 OS=Arabidopsis thaliana OX=3702 GN=At2g36330 PE=2 SV=1[more]
D7LIR23.2e-4037.59CASP-like protein 4A3 OS=Arabidopsis lyrata subsp. lyrata OX=81972 GN=ARALYDRAFT... [more]
Q9FNE83.1e-3538.71CASP-like protein 4A1 OS=Arabidopsis thaliana OX=3702 GN=At5g40300 PE=2 SV=1[more]
D7MMW42.7e-3436.95CASP-like protein 4A2 OS=Arabidopsis lyrata subsp. lyrata OX=81972 GN=ARALYDRAFT... [more]
Q501G61.1e-3235.93CASP-like protein 4A2 OS=Arabidopsis thaliana OX=3702 GN=At5g62820 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1JTE43.4e-14184.38CASP-like protein OS=Cucurbita maxima OX=3661 GN=LOC111489567 PE=3 SV=1[more]
A0A6J1FJD71.1e-13984.08CASP-like protein OS=Cucurbita moschata OX=3662 GN=LOC111444795 PE=3 SV=1[more]
A0A6J1BX269.9e-13380.18CASP-like protein OS=Momordica charantia OX=3673 GN=LOC111006289 PE=3 SV=1[more]
A0A0A0KS011.7e-13280.12CASP-like protein OS=Cucumis sativus OX=3659 GN=Csa_5G630810 PE=3 SV=1[more]
A0A5A7T7H81.9e-13181.27CASP-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold808G00... [more]
Match NameE-valueIdentityDescription
AT2G36330.14.2e-4338.64Uncharacterised protein family (UPF0497) [more]
AT5G40300.12.2e-3638.71Uncharacterised protein family (UPF0497) [more]
AT5G62820.17.9e-3435.93Uncharacterised protein family (UPF0497) [more]
AT2G38480.11.6e-1335.48Uncharacterised protein family (UPF0497) [more]
AT4G11655.12.9e-1233.55Uncharacterised protein family (UPF0497) [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 21..41
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 53..82
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..163
NoneNo IPR availablePANTHERPTHR11615:SF279CASP-LIKE PROTEIN 4A1coord: 94..332
NoneNo IPR availablePANTHERPTHR11615NITRATE, FORMATE, IRON DEHYDROGENASEcoord: 94..332
IPR006702Casparian strip membrane protein domainPFAMPF04535DUF588coord: 185..317
e-value: 1.4E-31
score: 109.3

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0029986.1Lag0029986.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006886 intracellular protein transport
biological_process GO:0016192 vesicle-mediated transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0030117 membrane coat
cellular_component GO:0005886 plasma membrane
molecular_function GO:0051539 4 iron, 4 sulfur cluster binding
molecular_function GO:0005198 structural molecule activity