Lag0029889 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0029889
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	RING-type E3 ubiquitin transferase
Location	chr8: 42894949 .. 42895482 (+)
RNA-Seq Expression	Lag0029889
Synteny	Lag0029889

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCCTCACCCAACGACGCCGCCTCTTACAGCCAAAAACTCGACATTTTCCTCATCGCCGTCGGCTCTGCCGGCGCCGTCGTCACCATTTACCACTGCATCATGGCTTGCTGGTGCGACCGCCGCCGCCTCAACGCCGGCGACGAAACGCCGGGTATCGACCTACACTCCCTGCCGCCGGAGGAGACGCCGCGGAGCACCGACAATTCCGTGGTCCACTTGATTCCGGCGCACAAGTACGAGAAGCGAATGGCGGTGGATGGCGGCGGGTGCGGCGGCGGCGACGGCGACGGCGAATGTGATGGCACGTGCGCGATCTGTTTGAGCGAGTTCGAAGAGGGGGAGGAAGTAAGGACGTTGCCGGAGTGTATGCATTCGTATCACGTGCCGTGTATCGACATGTGGCTTTACTCTCACTCGAGCTGTCCGATGTGTCGTACTGATGCCACTCCGACGTCGTTTCATGACAGGCTGCAGTTTGAGACGCAGCCCAGTTCTGGTATTACTGTGCACGTGCGGCCCACGAGGGATTGA

mRNA sequence

ATGTCCTCACCCAACGACGCCGCCTCTTACAGCCAAAAACTCGACATTTTCCTCATCGCCGTCGGCTCTGCCGGCGCCGTCGTCACCATTTACCACTGCATCATGGCTTGCTGGTGCGACCGCCGCCGCCTCAACGCCGGCGACGAAACGCCGGGTATCGACCTACACTCCCTGCCGCCGGAGGAGACGCCGCGGAGCACCGACAATTCCGTGGTCCACTTGATTCCGGCGCACAAGTACGAGAAGCGAATGGCGGTGGATGGCGGCGGGTGCGGCGGCGGCGACGGCGACGGCGAATGTGATGGCACGTGCGCGATCTGTTTGAGCGAGTTCGAAGAGGGGGAGGAAGTAAGGACGTTGCCGGAGTGTATGCATTCGTATCACGTGCCGTGTATCGACATGTGGCTTTACTCTCACTCGAGCTGTCCGATGTGTCGTACTGATGCCACTCCGACGTCGTTTCATGACAGGCTGCAGTTTGAGACGCAGCCCAGTTCTGGTATTACTGTGCACGTGCGGCCCACGAGGGATTGA

Coding sequence (CDS)

ATGTCCTCACCCAACGACGCCGCCTCTTACAGCCAAAAACTCGACATTTTCCTCATCGCCGTCGGCTCTGCCGGCGCCGTCGTCACCATTTACCACTGCATCATGGCTTGCTGGTGCGACCGCCGCCGCCTCAACGCCGGCGACGAAACGCCGGGTATCGACCTACACTCCCTGCCGCCGGAGGAGACGCCGCGGAGCACCGACAATTCCGTGGTCCACTTGATTCCGGCGCACAAGTACGAGAAGCGAATGGCGGTGGATGGCGGCGGGTGCGGCGGCGGCGACGGCGACGGCGAATGTGATGGCACGTGCGCGATCTGTTTGAGCGAGTTCGAAGAGGGGGAGGAAGTAAGGACGTTGCCGGAGTGTATGCATTCGTATCACGTGCCGTGTATCGACATGTGGCTTTACTCTCACTCGAGCTGTCCGATGTGTCGTACTGATGCCACTCCGACGTCGTTTCATGACAGGCTGCAGTTTGAGACGCAGCCCAGTTCTGGTATTACTGTGCACGTGCGGCCCACGAGGGATTGA

Protein sequence

MSSPNDAASYSQKLDIFLIAVGSAGAVVTIYHCIMACWCDRRRLNAGDETPGIDLHSLPPEETPRSTDNSVVHLIPAHKYEKRMAVDGGGCGGGDGDGECDGTCAICLSEFEEGEEVRTLPECMHSYHVPCIDMWLYSHSSCPMCRTDATPTSFHDRLQFETQPSSGITVHVRPTRD

Homology

[Close]

BLAST of Lag0029889 vs. NCBI nr
Match: XP_022939139.1 (RING-H2 finger protein ATL52-like [Cucurbita moschata] >KAG7016242.1 RING-H2 finger protein ATL52, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 313.5 bits (802), Expect = 1.1e-81
Identity = 148/175 (84.57%), Postives = 156/175 (89.14%), Query Frame = 0


Query: 3   SPNDAASYSQKLDIFLIAVGSAGAVVTIYHCIMACWCDRRRLNAGDETPGIDLHSLPPEE 62
           S +DAA YSQKLDIFLIAVGSAGAVVTIYHCIMACWCDRRRLNAGD+TP  DLHS+   E
Sbjct: 2   SSSDAAVYSQKLDIFLIAVGSAGAVVTIYHCIMACWCDRRRLNAGDQTPANDLHSMSLAE 61

Query: 63  TPRSTDNSVVHLIPAHKYEKRMAVDGGGCGGGDGDGECDGTCAICLSEFEEGEEVRTLPE 122
           TPRST+NSVVHLIPAHKYEK+ AVD GGC G       DGTCAICLSEFEEGEE+RTLPE
Sbjct: 62  TPRSTENSVVHLIPAHKYEKQKAVDSGGCDG-------DGTCAICLSEFEEGEEIRTLPE 121

Query: 123 CMHSYHVPCIDMWLYSHSSCPMCRTDATPTSFHDRLQFETQPSSGITVHVRPTRD 178
           C+HSYHVPCIDMWLYSHSSCPMCRTDATPTS HDRLQFETQPSSGI VHVRPTR+
Sbjct: 122 CLHSYHVPCIDMWLYSHSSCPMCRTDATPTSVHDRLQFETQPSSGIIVHVRPTRN 169

[Close]

BLAST of Lag0029889 vs. NCBI nr
Match: XP_038889238.1 (probable E3 ubiquitin-protein ligase ATL44 [Benincasa hispida])

HSP 1 Score: 312.0 bits (798), Expect = 3.3e-81
Identity = 144/172 (83.72%), Postives = 155/172 (90.12%), Query Frame = 0


Query: 3   SPNDAASYSQKLDIFLIAVGSAGAVVTIYHCIMACWCDRRRLNAGDETPGIDLHSLPPEE 62
           S  DA  YS+KLDIFL+A+GSAGAVVTIYHCIMACWCDRRR N+G +TP  D HSLP  +
Sbjct: 2   SDTDATIYSRKLDIFLVAIGSAGAVVTIYHCIMACWCDRRRFNSGGQTPANDPHSLPITD 61

Query: 63  TPRSTDNSVVHLIPAHKYEKRMAVDGGGCGGGDGDGECDGTCAICLSEFEEGEEVRTLPE 122
           TPRST+NSVVHLIPAHKYEKRMAVDGGGCGG  G+G  DGTCAICLSEFEEGEE++TLPE
Sbjct: 62  TPRSTENSVVHLIPAHKYEKRMAVDGGGCGGDHGEG--DGTCAICLSEFEEGEEIKTLPE 121

Query: 123 CMHSYHVPCIDMWLYSHSSCPMCRTDATPTSFHDRLQFETQPSSGITVHVRP 175
           CMHSYHVPCIDMWLYSHSSCPMCRT+ATPTSFHDRLQFETQPSSGI VHV P
Sbjct: 122 CMHSYHVPCIDMWLYSHSSCPMCRTNATPTSFHDRLQFETQPSSGIVVHVLP 171

[Close]

BLAST of Lag0029889 vs. NCBI nr
Match: XP_023551520.1 (probable E3 ubiquitin-protein ligase ATL44 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 311.2 bits (796), Expect = 5.6e-81
Identity = 147/175 (84.00%), Postives = 156/175 (89.14%), Query Frame = 0


Query: 3   SPNDAASYSQKLDIFLIAVGSAGAVVTIYHCIMACWCDRRRLNAGDETPGIDLHSLPPEE 62
           S +DAA YSQKLDIFLIAVGSAGAVVTIYHCIMACWCDRRRLNAGD+TP  DLHS+   E
Sbjct: 2   SSSDAAVYSQKLDIFLIAVGSAGAVVTIYHCIMACWCDRRRLNAGDQTPANDLHSMSLAE 61

Query: 63  TPRSTDNSVVHLIPAHKYEKRMAVDGGGCGGGDGDGECDGTCAICLSEFEEGEEVRTLPE 122
           TPRST+NSVVHLIPA+KYEK+ AVD GGC G       DGTCAICLSEFEEGEE+RTLPE
Sbjct: 62  TPRSTENSVVHLIPAYKYEKQKAVDSGGCDG-------DGTCAICLSEFEEGEEIRTLPE 121

Query: 123 CMHSYHVPCIDMWLYSHSSCPMCRTDATPTSFHDRLQFETQPSSGITVHVRPTRD 178
           C+HSYHVPCIDMWLYSHSSCPMCRTDATPTS HDRLQFETQPSSGI VHVRPTR+
Sbjct: 122 CLHSYHVPCIDMWLYSHSSCPMCRTDATPTSVHDRLQFETQPSSGIIVHVRPTRN 169

[Close]

BLAST of Lag0029889 vs. NCBI nr
Match: KAG6578710.1 (putative E3 ubiquitin-protein ligase ATL44, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 303.1 bits (775), Expect = 1.5e-78
Identity = 143/169 (84.62%), Postives = 150/169 (88.76%), Query Frame = 0


Query: 3   SPNDAASYSQKLDIFLIAVGSAGAVVTIYHCIMACWCDRRRLNAGDETPGIDLHSLPPEE 62
           S +DAA YSQKLDIFLIAVGSAGAVVTIYHCIMACWCDRRRLNAGD+TP  DLHS+   E
Sbjct: 2   SSSDAAVYSQKLDIFLIAVGSAGAVVTIYHCIMACWCDRRRLNAGDQTPANDLHSMSLAE 61

Query: 63  TPRSTDNSVVHLIPAHKYEKRMAVDGGGCGGGDGDGECDGTCAICLSEFEEGEEVRTLPE 122
           TPRST+NSVVHLIPAHKYEK+ AVD GGC G       DGTCAICLSEFEEGEE+RTLPE
Sbjct: 62  TPRSTENSVVHLIPAHKYEKQKAVDSGGCDG-------DGTCAICLSEFEEGEEIRTLPE 121

Query: 123 CMHSYHVPCIDMWLYSHSSCPMCRTDATPTSFHDRLQFETQPSSGITVH 172
           C+HSYHVPCIDMWLYSHSSCPMCRTDATPTS HDRLQFETQPSSGI VH
Sbjct: 122 CLHSYHVPCIDMWLYSHSSCPMCRTDATPTSVHDRLQFETQPSSGIIVH 163

[Close]

BLAST of Lag0029889 vs. NCBI nr
Match: XP_008458935.1 (PREDICTED: RING-H2 finger protein ATL52-like [Cucumis melo] >KAA0042487.1 RING-H2 finger protein ATL52-like [Cucumis melo var. makuwa] >TYK22889.1 RING-H2 finger protein ATL52-like [Cucumis melo var. makuwa])

HSP 1 Score: 296.6 bits (758), Expect = 1.4e-76
Identity = 141/176 (80.11%), Postives = 156/176 (88.64%), Query Frame = 0


Query: 2   SSPNDAASYSQKLDIFLIAVGSAGAVVTIYHCIMACWCDRRRLNAGDETPGIDLHSLPPE 61
           SS  DAA YS+KLDIFLIA+GSAGAVVTIYHCIMACWCDRRR  +GD++   D H+LP  
Sbjct: 3   SSTGDAAIYSRKLDIFLIAIGSAGAVVTIYHCIMACWCDRRRFTSGDQSSANDPHTLPIT 62

Query: 62  ETPRSTDNSVVHLIPAHKYEKRMAVDGGGCG-GGDGDGECDGTCAICLSEFEEGEEVRTL 121
           +TPRST+NSVVHLIPAHKYEKRMAVDGGG G G D DG  DGTCAICLSEFEEGEE++TL
Sbjct: 63  DTPRSTENSVVHLIPAHKYEKRMAVDGGGGGVGKDVDG--DGTCAICLSEFEEGEEIKTL 122

Query: 122 PECMHSYHVPCIDMWLYSHSSCPMCRTDATPTSFHDRLQFETQPSSGITVHVRPTR 177
           PEC HSYH+PCIDMWLYSHSSCPMCRT+ATPTSF+DRLQF+TQP+SGI VHV PTR
Sbjct: 123 PECKHSYHMPCIDMWLYSHSSCPMCRTNATPTSFNDRLQFQTQPNSGIVVHVLPTR 176

[Close]

BLAST of Lag0029889 vs. ExPASy Swiss-Prot
Match: Q9LF64 (RING-H2 finger protein ATL52 OS=Arabidopsis thaliana OX=3702 GN=ATL52 PE=2 SV=1)

HSP 1 Score: 92.4 bits (228), Expect = 5.4e-18
Identity = 53/156 (33.97%), Postives = 86/156 (55.13%), Query Frame = 0


Query: 3   SPNDAASYSQKLDIFLIAVGSAGAVVTIYHCIMACWCDRRRLNAGDETPGIDLHSLP-PE 62
           S + ++S+S  L I LI + ++  ++  Y+ +++ +C R    +  ET  ++ +      
Sbjct: 47  SSSSSSSFSPLL-IALIGILTSALILVSYYTLISKYCHRHHQTSSSETLNLNHNGEGFFS 106

Query: 63  ETPRSTDN------SVVHLIPAHKYEKRMAVDGGGCGGGDGDGECDGT-CAICLSEFEEG 122
            T R + N      S++  I  +KY+              GDG  DG+ C++CLSEFEE 
Sbjct: 107 STQRISTNGDGLNESMIKSITVYKYK-------------SGDGFVDGSDCSVCLSEFEEN 166

Query: 123 EEVRTLPECMHSYHVPCIDMWLYSHSSCPMCRTDAT 151
           E +R LP+C H++H+PCID WL SHS+CP+CR   T
Sbjct: 167 ESLRLLPKCNHAFHLPCIDTWLKSHSNCPLCRAFVT 188

[Close]

BLAST of Lag0029889 vs. ExPASy Swiss-Prot
Match: P93823 (RING-H2 finger protein ATL1 OS=Arabidopsis thaliana OX=3702 GN=ATL1 PE=2 SV=1)

HSP 1 Score: 89.4 bits (220), Expect = 4.6e-17
Identity = 44/137 (32.12%), Postives = 73/137 (53.28%), Query Frame = 0


Query: 21  VGSAGAVVTIYHCIMACWCD-------RRRLNAGDETPGIDLHSLPPEETPRSTDNSVVH 80
           + +A  +V+ Y  ++ C  +       RRR  + D+ P   L    P E  R  D S + 
Sbjct: 52  LATAFLLVSYYIFVIKCCLNWHQIDIFRRRRRSSDQNP---LMIYSPHEVNRGLDESAIR 111

Query: 81  LIPAHKYEKRMAVDGGGCGGGDGDGECDGTCAICLSEFEEGEEVRTLPECMHSYHVPCID 140
            IP  K++KR  V      G +   +    C++CL+EF+E E++R +P C H +H+ CID
Sbjct: 112 AIPVFKFKKRDVV-----AGEEDQSKNSQECSVCLNEFQEDEKLRIIPNCCHVFHIDCID 171

Query: 141 MWLYSHSSCPMCRTDAT 151
           +WL  +++CP+CRT  +
Sbjct: 172 IWLQGNANCPLCRTSVS 180

[Close]

BLAST of Lag0029889 vs. ExPASy Swiss-Prot
Match: Q9LSW9 (RING-H2 finger protein ATL16 OS=Arabidopsis thaliana OX=3702 GN=ATL16 PE=2 SV=1)

HSP 1 Score: 84.3 bits (207), Expect = 1.5e-15
Identity = 48/117 (41.03%), Postives = 70/117 (59.83%), Query Frame = 0


Query: 41  RRRLNAGDETPGIDLHSLPPEETPRSTDNSVVHLIPAHKYEKRM-AVDGGGCGGGDGDGE 100
           RRR N  D+ P   L    PE   R  D SV+  IP  K++KR    DG   G G+ + E
Sbjct: 78  RRRRN--DQDP---LMVYSPELRSRGLDESVIRAIPIFKFKKRYDQNDGVFTGEGEEEEE 137

Query: 101 -CDGTCAICLSEFEEGEEVRTLPECMHSYHVPCIDMWLYSHSSCPMCRTDAT-PTSF 155
                C++CLSEF++ E++R +P C H +H+ CID+WL ++++CP+CRT  +  TSF
Sbjct: 138 KRSQECSVCLSEFQDEEKLRIIPNCSHLFHIDCIDVWLQNNANCPLCRTRVSCDTSF 189

[Close]

BLAST of Lag0029889 vs. ExPASy Swiss-Prot
Match: Q9SRQ8 (RING-H2 finger protein ATL51 OS=Arabidopsis thaliana OX=3702 GN=ATL51 PE=2 SV=2)

HSP 1 Score: 84.3 bits (207), Expect = 1.5e-15
Identity = 51/162 (31.48%), Postives = 82/162 (50.62%), Query Frame = 0


Query: 5   NDAASYSQKLDIFLIAVGSAGAVVTIYHCIMACWCDRRRLNAGDETPGIDLHSLPPEETP 64
           + ++S    L I LI + ++  ++  Y+ +++ +C RRR N+   T    ++ +  + T 
Sbjct: 50  DSSSSDFSPLLIALIGILASAFILVSYYTLISKYCHRRRHNS-SSTSAAAINRISSDYTW 109

Query: 65  RSTDNSVVHLIPAHKYEKRMAVDGGGCGGGDGDG---------------ECDG-----TC 124
           + T+N+                     GGG GDG               + DG      C
Sbjct: 110 QGTNNN---------NNNGATNPNQTIGGGGGDGLDESLIKSITVYKYRKMDGFVESSDC 169

Query: 125 AICLSEFEEGEEVRTLPECMHSYHVPCIDMWLYSHSSCPMCR 147
           ++CLSEF+E E +R LP+C H++HVPCID WL SHS+CP+CR
Sbjct: 170 SVCLSEFQENESLRLLPKCNHAFHVPCIDTWLKSHSNCPLCR 201

[Close]

BLAST of Lag0029889 vs. ExPASy Swiss-Prot
Match: Q67YI6 (RING-H2 finger protein ATL65 OS=Arabidopsis thaliana OX=3702 GN=ATL65 PE=2 SV=2)

HSP 1 Score: 83.2 bits (204), Expect = 3.3e-15
Identity = 49/123 (39.84%), Postives = 61/123 (49.59%), Query Frame = 0


Query: 39  CDRRRL-----NAGDETPGIDLHSLPP---------EETPRSTDNSVVHLIPAHKYEKRM 98
           C RRRL      +   T   DL S  P           +P   D+SV+  +P   Y    
Sbjct: 79  CRRRRLLHLSSASSASTSSSDLRSFSPFPFDSFHYSSYSPYGLDDSVIKTLPLFLY-SAA 138

Query: 99  AVDGGGCGGGDGDGECDGTCAICLSEFEEGEEVRTLPECMHSYHVPCIDMWLYSHSSCPM 148
           A  G    G      C   CA+CL EFEEG+ VRTLP C H++H+ CID WL SH +CP+
Sbjct: 139 ACTGKPAVGKTSAANC-RDCAVCLLEFEEGDYVRTLPLCFHAFHLECIDEWLRSHPNCPL 198

The following BLAST results are available for this feature:

BLAST of Lag0029889 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022939139.1	1.1e-81	84.57	RING-H2 finger protein ATL52-like [Cucurbita moschata] >KAG7016242.1 RING-H2 fin...	[more]
XP_038889238.1	3.3e-81	83.72	probable E3 ubiquitin-protein ligase ATL44 [Benincasa hispida]	[more]
XP_023551520.1	5.6e-81	84.00	probable E3 ubiquitin-protein ligase ATL44 [Cucurbita pepo subsp. pepo]	[more]
KAG6578710.1	1.5e-78	84.62	putative E3 ubiquitin-protein ligase ATL44, partial [Cucurbita argyrosperma subs...	[more]
XP_008458935.1	1.4e-76	80.11	PREDICTED: RING-H2 finger protein ATL52-like [Cucumis melo] >KAA0042487.1 RING-H...	[more]

BLAST of Lag0029889 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LF64	5.4e-18	33.97	RING-H2 finger protein ATL52 OS=Arabidopsis thaliana OX=3702 GN=ATL52 PE=2 SV=1	[more]
P93823	4.6e-17	32.12	RING-H2 finger protein ATL1 OS=Arabidopsis thaliana OX=3702 GN=ATL1 PE=2 SV=1	[more]
Q9LSW9	1.5e-15	41.03	RING-H2 finger protein ATL16 OS=Arabidopsis thaliana OX=3702 GN=ATL16 PE=2 SV=1	[more]
Q9SRQ8	1.5e-15	31.48	RING-H2 finger protein ATL51 OS=Arabidopsis thaliana OX=3702 GN=ATL51 PE=2 SV=2	[more]
Q67YI6	3.3e-15	39.84	RING-H2 finger protein ATL65 OS=Arabidopsis thaliana OX=3702 GN=ATL65 PE=2 SV=2	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 104..145 e-value: 2.6E-7 score: 40.3
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 103..146 e-value: 2.7E-13 score: 50.0
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 104..146 score: 12.267982
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 57..152 e-value: 7.6E-21 score: 75.6
None	No IPR available	PANTHER	PTHR45676:SF43	RING-H2 FINGER PROTEIN ATL52-LIKE	coord: 9..158
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 99..151
IPR044675	E3 ubiquitin-protein ligase RING1-like	PANTHER	PTHR45676	RING-H2 FINGER PROTEIN ATL51-RELATED	coord: 9..158

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0029889.1	Lag0029889.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016567	protein ubiquitination
cellular_component	GO:0016021	integral component of membrane